BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00001351
         (1910 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002275712.2| PREDICTED: RING finger protein 10-like [Viti...   608   0.0  
ref|XP_002320428.1| predicted protein [Populus trichocarpa] gi|2...   577   e-162
ref|XP_002512841.1| conserved hypothetical protein [Ricinus comm...   575   e-161
ref|XP_003624500.1| RING finger protein [Medicago truncatula] gi...   571   e-160
ref|XP_002875336.1| hypothetical protein ARALYDRAFT_484446 [Arab...   546   e-156

>ref|XP_002275712.2| PREDICTED: RING finger protein 10-like [Vitis vinifera]
          Length = 765

 Score =  608 bits (1567), Expect(2) = 0.0
 Identities = 330/585 (56%), Positives = 391/585 (66%), Gaps = 22/585 (3%)
 Frame = -1

Query: 1910 SLHISHHISGPVAPTAENSGGSSGKMNEARKPYGRKGYTHHKAGTSLQRQNAGQTGVASA 1731
            S+  S  +SG +AP A +SGGSS K+ E      +K   H   GT  Q ++ G+ G  S 
Sbjct: 63   SVASSEDVSGSLAPAAADSGGSSKKVTELGVQNSKKSLPHQNTGTRSQSRSVGR-GSEST 121

Query: 1730 QHRGNXXXXXXXXXXXXXXXXXXXG--------KKGQMVNGNHLLNFQYDPIXXXXXXXX 1575
            Q RG                             +K QM NGNHLLNF YDPI        
Sbjct: 122  QSRGKLSGPTISPRSQQASGSINFHGSTTHSAGRKTQM-NGNHLLNFHYDPISRPQPRAP 180

Query: 1574 XXXXXXXXXXXSYNKDLFLQANYKFVLLDSGNYAPDTMDPDKALHWEDIICVKYSTPLPV 1395
                        YNKDLFLQANY+FV+LD+GN  P+ MDPDK L W+DIICV+YSTPLPV
Sbjct: 181  PPRRQQKIKP--YNKDLFLQANYRFVVLDTGNQVPELMDPDKMLQWDDIICVRYSTPLPV 238

Query: 1394 QCPVCLEDPLCPQITSCGHIFCFPCILRYFSVGEDDFKVDCCKKCPLCFMMISIKDLYTI 1215
            QCP+CLE PLCPQITSCGHIFCFPCILRY  +G++D K DC KKCPLCF+MIS KDLYT+
Sbjct: 239  QCPICLECPLCPQITSCGHIFCFPCILRYLLMGKEDHKGDCWKKCPLCFVMISPKDLYTL 298

Query: 1214 YIENVKQYSLGDVIEFMLLTRQKDSFTLALKNKQG---MCSVEEVHDSFSKFTFTSDVDL 1044
            YIENVKQY +GD  EFMLLTRQKDSFTL+ KNKQ    M    EVHDSFSKF FTSDVDL
Sbjct: 299  YIENVKQYCVGDTAEFMLLTRQKDSFTLSEKNKQETDVMPCNSEVHDSFSKFIFTSDVDL 358

Query: 1043 SVREAISELDNWLAKADSDLVDDLSMLPYVCAAREQLEQRKTSWNEH----------QIG 894
            SVREAIS+LD+WL +ADS LVDDL  LPYVCAA EQLEQRK  WNEH             
Sbjct: 359  SVREAISDLDSWLVRADSGLVDDLEKLPYVCAAVEQLEQRKKYWNEHGTCKSTISVKYTD 418

Query: 893  CGNGDDSAGNISGHTRSPL-AKNLLSGILSASVNDKPTCVEGSSHNPKEGESCMAQVDDV 717
            C +G     + +    S   A     G  S+ V+DK   +   + +  +G  C+ Q  D+
Sbjct: 419  CQSGSHVIPSTTNVACSDYEACGFKYGSPSSDVDDKSKWLGNLTPDKLDGGECLVQTADM 478

Query: 716  IRSPEVCGGALSSSYNDDKSVQMHANGFRDRKDNDSYNFYQAVDGQHLILHPINLKCLLH 537
              + E    ALSSSY+++KS+Q H++G RD KD+DSYNFYQAVDGQHLILHP+N+KCLLH
Sbjct: 479  GETLEGQCRALSSSYDENKSLQRHSSGSRDLKDDDSYNFYQAVDGQHLILHPLNMKCLLH 538

Query: 536  HYGSYDRLPHRIHGKILQLESVTQSEAMRRRYRYLSHFSLTTTFQLCEIDLSDSLPFDAL 357
            HYG Y  LP+RI G+ILQLE+VTQSEAMRRRYRYLSHFSLTTTFQLCEIDL + LP  A 
Sbjct: 539  HYGGYAFLPNRISGRILQLETVTQSEAMRRRYRYLSHFSLTTTFQLCEIDLREVLPPSAF 598

Query: 356  SPFIDEIKNREKQRKRTARKEQEEKIKAEVAAATEYLTMPYNFSQ 222
             PF+DEIK RE+QRK+ ARK+ +EK+KAE A  T+ L +P  F +
Sbjct: 599  FPFMDEIKKRERQRKKLARKDTKEKLKAE-ATTTDSLPIPSRFGE 642



 Score = 62.8 bits (151), Expect(2) = 0.0
 Identities = 35/60 (58%), Positives = 40/60 (66%), Gaps = 6/60 (10%)
 Frame = -2

Query: 166 SNVTRLGYAAAHDSPALKFEEPRGVPKTDFASNSS------PSGTQSFANVISKAKPVES 5
           SNVT+LG+AA HDSP LK EE   V  T  AS+SS       +GT SFANVIS+ K VES
Sbjct: 677 SNVTKLGFAAGHDSPGLKIEEVHSVHSTSMASDSSGVTGSRNAGTPSFANVISRGKSVES 736


>ref|XP_002320428.1| predicted protein [Populus trichocarpa] gi|222861201|gb|EEE98743.1|
            predicted protein [Populus trichocarpa]
          Length = 622

 Score =  577 bits (1488), Expect = e-162
 Identities = 295/476 (61%), Positives = 355/476 (74%), Gaps = 10/476 (2%)
 Frame = -1

Query: 1655 KKGQMVNGNHLLNFQYDPIXXXXXXXXXXXXXXXXXXXSYNKDLFLQANYKFVLLDSGNY 1476
            ++ QM N NHLLNF YDPI                    YNKDLFLQANYKFV+LD+G+Y
Sbjct: 24   RRAQMTNANHLLNFHYDPISRPQPRAPPPRRPQKKRA--YNKDLFLQANYKFVVLDTGSY 81

Query: 1475 APDTMDPDKALHWEDIICVKYSTPLPVQCPVCLEDPLCPQITSCGHIFCFPCILRYFSVG 1296
            AP++MDPDK L WEDIICV+YST  PVQCP+CLE PLCPQITSCGHIFCFPCILRY  +G
Sbjct: 82   APESMDPDKMLKWEDIICVRYSTTFPVQCPICLEYPLCPQITSCGHIFCFPCILRYLLMG 141

Query: 1295 EDDFKVDCCKKCPLCFMMISIKDLYTIYIENVKQYSLGDVIEFMLLTRQKDSFTLALKNK 1116
            E+D K DC K+CPLCF+MIS KDLYTIYIE VKQY +G+ IEFMLLTRQK SF  ++K +
Sbjct: 142  EEDHKGDCFKRCPLCFVMISPKDLYTIYIEKVKQYCIGETIEFMLLTRQKHSFVPSMKIE 201

Query: 1115 ----QGMCSVEEVHDSFSKFTFTSDVDLSVREAISELDNWLAKADSDLVDDLSMLPYVCA 948
                  +CS + ++D FSKFTFTSDV+LSVR+AIS+LD+WL +ADS LVDDL  LPYVCA
Sbjct: 202  PEADTELCSNDNIYDPFSKFTFTSDVELSVRKAISDLDSWLVRADSGLVDDLEKLPYVCA 261

Query: 947  AREQLEQRKTSWNEHQIGCGNGDDSAGNISGHTRSPLAKNLLSGILSASVNDKPTCVEGS 768
            A EQLE+RK  WNE Q  C    D   N+S   +   ++ LLS + +A    K  C   S
Sbjct: 262  AMEQLEKRKKYWNE-QKAC---HDDRFNVSNSQKG--SQGLLSSLTTAKGEHK-ACSSRS 314

Query: 767  S------HNPKEGESCMAQVDDVIRSPEVCGGALSSSYNDDKSVQMHANGFRDRKDNDSY 606
            +      +N  +G   +  + DV+ SPE    ALSSSY ++K+ Q HANG+RD KD +SY
Sbjct: 315  ATPSIDINNKNKGSDNV--MGDVVESPEDEDSALSSSYEENKNFQTHANGYRDVKDKESY 372

Query: 605  NFYQAVDGQHLILHPINLKCLLHHYGSYDRLPHRIHGKILQLESVTQSEAMRRRYRYLSH 426
            NFYQA+DGQHLILHP+N+KCLLHHYGSYD LPHR+ G ILQLE++TQSEAMRRRYRYLSH
Sbjct: 373  NFYQAIDGQHLILHPLNMKCLLHHYGSYDLLPHRVSGTILQLETITQSEAMRRRYRYLSH 432

Query: 425  FSLTTTFQLCEIDLSDSLPFDALSPFIDEIKNREKQRKRTARKEQEEKIKAEVAAA 258
            FSLTTTFQLCEIDL+ +LP +AL PF+DEIK REK RK+ A KE++EKIKAE +A+
Sbjct: 433  FSLTTTFQLCEIDLNAALPPNALLPFMDEIKKREKLRKQLANKERKEKIKAEASAS 488


>ref|XP_002512841.1| conserved hypothetical protein [Ricinus communis]
            gi|223547852|gb|EEF49344.1| conserved hypothetical
            protein [Ricinus communis]
          Length = 746

 Score =  575 bits (1481), Expect = e-161
 Identities = 310/550 (56%), Positives = 364/550 (66%), Gaps = 8/550 (1%)
 Frame = -1

Query: 1886 SGPVAPTAENSGGSSGKMNEARKPYGRKGYTHHKAGTSLQRQNAGQ--TGVASAQHRGNX 1713
            SG  AP AE SGGSS ++N +R    +K           Q +  GQ   G  S     + 
Sbjct: 63   SGQFAPAAEESGGSSKQVNGSRTLNEKKSLPQQNGEKHSQSRLDGQGSKGKTSGTMSSSQ 122

Query: 1712 XXXXXXXXXXXXXXXXXXGKKGQMVNGNHLLNFQYDPIXXXXXXXXXXXXXXXXXXXSYN 1533
                              G++ QM+N NHLLNF YDPI                    YN
Sbjct: 123  GQQSNGPINGQGSSAYSSGRRAQMMNANHLLNFHYDPICRPKPRAPPPRRQQKIKP--YN 180

Query: 1532 KDLFLQANYKFVLLDSGNYAPDTMDPDKALHWEDIICVKYSTPLPVQCPVCLEDPLCPQI 1353
            KDLFLQANYKFVLLD GNY P +MDPDK L W+DIICV+Y+TP+PVQCP+CLE PLCPQI
Sbjct: 181  KDLFLQANYKFVLLDKGNYTPASMDPDKMLIWDDIICVRYATPVPVQCPICLEYPLCPQI 240

Query: 1352 TSCGHIFCFPCILRYFSVGEDDFKVDCCKKCPLCFMMISIKDLYTIYIENVKQYSLGDVI 1173
            TSCGHIFCFPCILRY  +G++D K DC K+CPLCF+M+S KDLYTIYIENVKQYS+G+ +
Sbjct: 241  TSCGHIFCFPCILRYLLLGKEDHKGDCFKRCPLCFVMVSQKDLYTIYIENVKQYSVGNTV 300

Query: 1172 EFMLLTRQKDSFTLALKNK---QGMCSVEEVHDSFSKFTFTSDVDLSVREAISELDNWLA 1002
            +FMLLTRQKDS T +LK K       S  ++++ FSKFTFTSD++LSVR+AI++LD WLA
Sbjct: 301  DFMLLTRQKDSITPSLKTKTERDTFMSCSDIYNPFSKFTFTSDLELSVRKAIADLDGWLA 360

Query: 1001 KADSDLVDDLSMLPYVCAAREQLEQRKTSWNEHQIGCGNGDDSAGNISGHTRSPLAKNLL 822
            KADS LVDDL  LPYVCAA E+LEQRK  WNE         D++ N   H  S      L
Sbjct: 361  KADSGLVDDLEKLPYVCAAMEKLEQRKKYWNEQW-----DSDNSRNYK-HANSQKQSQKL 414

Query: 821  SGILSASVNDKPTCVEGSSHNP---KEGESCMAQVDDVIRSPEVCGGALSSSYNDDKSVQ 651
               +  +  D      GS   P   K+         D + + E  G   SSS  + KS+Q
Sbjct: 415  LSTVDVNCADDEGGNTGSLIRPIDLKDENKWSEFKSDAVMTVEGQGTTFSSSCTESKSLQ 474

Query: 650  MHANGFRDRKDNDSYNFYQAVDGQHLILHPINLKCLLHHYGSYDRLPHRIHGKILQLESV 471
              +N  RD KD+DSY FYQAVDGQHLILHP+N+KCLL HYGSYD LPHRI GKILQLE V
Sbjct: 475  KISNESRDMKDSDSYYFYQAVDGQHLILHPLNMKCLLQHYGSYDMLPHRISGKILQLEVV 534

Query: 470  TQSEAMRRRYRYLSHFSLTTTFQLCEIDLSDSLPFDALSPFIDEIKNREKQRKRTARKEQ 291
            TQSEAMRRRYRYLSHFSLTTTFQLCEIDLS+ LP DAL PF+DEIK REKQRK  A+KE+
Sbjct: 535  TQSEAMRRRYRYLSHFSLTTTFQLCEIDLSEVLPPDALFPFMDEIKKREKQRKELAKKER 594

Query: 290  EEKIKAEVAA 261
            +EKIKAE AA
Sbjct: 595  KEKIKAEAAA 604


>ref|XP_003624500.1| RING finger protein [Medicago truncatula] gi|355499515|gb|AES80718.1|
            RING finger protein [Medicago truncatula]
          Length = 744

 Score =  571 bits (1472), Expect = e-160
 Identities = 309/572 (54%), Positives = 375/572 (65%), Gaps = 30/572 (5%)
 Frame = -1

Query: 1895 HHISGPVAPTAENSGGSSGKMNEARKPYGRKGYTHHKAGTSLQRQNAGQTGVASAQHRG- 1719
            H + GP  P AENSG  S K+ E     G K  T  +   +  R ++G+  V S+Q+ G 
Sbjct: 47   HDLPGPSNPAAENSGVPSEKVTELESSSGMK--TPQRNSRTQSRSHSGRRTVGSSQYEGK 104

Query: 1718 ------NXXXXXXXXXXXXXXXXXXXGKKGQMVNGNHLLNFQYDPIXXXXXXXXXXXXXX 1557
                  +                   G+K QM N NHLLNFQYDPI              
Sbjct: 105  TTGTVSSHRNQQTTGSVYSQGSTPLAGRKSQMANANHLLNFQYDPISRSQQRGPPPPPPA 164

Query: 1556 XXXXXS--YNKDLFLQANYKFVLLDSGNYAPDTMDPDKALHWEDIICVKYSTPLPVQCPV 1383
                 +  YNKDLFLQAN+KF++LDSGNY+PD+MDPDK L WEDIICV YSTP PVQCP+
Sbjct: 165  RRQRKTRPYNKDLFLQANFKFMVLDSGNYSPDSMDPDKMLQWEDIICVTYSTPFPVQCPI 224

Query: 1382 CLEDPLCPQITSCGHIFCFPCILRYFSVGEDDFKVDCCKKCPLCFMMISIKDLYTIYIEN 1203
            CLE PLCPQITSCGHIFCFPCIL+Y  +GE+D K D  K+CPLCF+ IS+KDLYT++I N
Sbjct: 225  CLEHPLCPQITSCGHIFCFPCILQYLMLGEEDHKGDRWKRCPLCFVTISVKDLYTLHITN 284

Query: 1202 VKQYSLGDVIEFMLLTRQKDSFTLALKNKQ----GMCSVEEVHDSFSKFTFTSDVDLSVR 1035
            VKQY +GD IEF  LTR+KDSFTL+ KNKQ      C   +V D FSKFT TSDVDLSVR
Sbjct: 285  VKQYQVGDNIEFTFLTRKKDSFTLSHKNKQEGDNSSCGRGDVCDPFSKFTLTSDVDLSVR 344

Query: 1034 EAISELDNWLAKADSDLVDDLSMLPYVCAAREQLEQRKTSWNEHQIGCGNGDDSAGNISG 855
             AIS+LD WLA+ADS LVDD+  LPYV AA +QL+QRK  WNEH+  C   + S+  I  
Sbjct: 345  HAISDLDGWLARADSGLVDDMEKLPYVSAAMQQLKQRKKYWNEHK-AC-YSEKSSNLIDY 402

Query: 854  HTRSPLAKNLLSGILSASVNDKPTCVEGSSHNPKE--------------GESCM---AQV 726
              ++P      S   +A  +D  +C  GS  +  +                +C      V
Sbjct: 403  APQTP------SISTNAWDSDDESCSNGSRTSSTDFPDQSKVVILDKSTAGTCQDDTLDV 456

Query: 725  DDVIRSPEVCGGALSSSYNDDKSVQMHANGFRDRKDNDSYNFYQAVDGQHLILHPINLKC 546
            D V+   E+    LSSSY +   +Q H+NG  D K+NDSYNFYQAVDGQHLILHP+N KC
Sbjct: 457  DKVLAEKEI---TLSSSYEEKNCIQGHSNGIGDAKENDSYNFYQAVDGQHLILHPLNTKC 513

Query: 545  LLHHYGSYDRLPHRIHGKILQLESVTQSEAMRRRYRYLSHFSLTTTFQLCEIDLSDSLPF 366
            LLHHYGSYD+LPHRI G+ILQLE+VTQSEAMRRRYR+LSHF LTT FQLCE+DLS+ LP 
Sbjct: 514  LLHHYGSYDKLPHRISGRILQLETVTQSEAMRRRYRFLSHFPLTTIFQLCEVDLSEMLPP 573

Query: 365  DALSPFIDEIKNREKQRKRTARKEQEEKIKAE 270
            +AL+PF+DEIK R  QRK+ A+KEQ+EK+KAE
Sbjct: 574  EALAPFMDEIKKRANQRKQIAKKEQKEKLKAE 605


>ref|XP_002875336.1| hypothetical protein ARALYDRAFT_484446 [Arabidopsis lyrata subsp.
            lyrata] gi|297321174|gb|EFH51595.1| hypothetical protein
            ARALYDRAFT_484446 [Arabidopsis lyrata subsp. lyrata]
          Length = 760

 Score =  546 bits (1407), Expect(2) = e-156
 Identities = 273/491 (55%), Positives = 348/491 (70%), Gaps = 13/491 (2%)
 Frame = -1

Query: 1655 KKGQMVNGNHLLNFQYDPIXXXXXXXXXXXXXXXXXXXSYNKDLFLQANYKFVLLDSGNY 1476
            ++  M++GNHLLNFQYDPI                    +NKDLFL+ANYKFV+LD+G++
Sbjct: 149  RRANMISGNHLLNFQYDPISPPQSRGPPPQRRQQYKGRPFNKDLFLKANYKFVVLDTGDH 208

Query: 1475 APDTMDPDKALHWEDIICVKYSTPLPVQCPVCLEDPLCPQITSCGHIFCFPCILRYFSVG 1296
            +PD+MDPDK L W+DIICV+YSTP PVQCP+CLE PLCPQIT+CGHIFCFPCIL+Y  +G
Sbjct: 209  SPDSMDPDKMLQWDDIICVRYSTPSPVQCPICLEYPLCPQITACGHIFCFPCILQYLLIG 268

Query: 1295 EDDFKVDCCKKCPLCFMMISIKDLYTIYIENVKQYSLGDVIEFMLLTRQKDSFTLALKNK 1116
             D+ KVDC K+CPLCF+MIS ++LYT+YIENVKQ S+GD IEF+LLTR+KDSF    KN+
Sbjct: 269  VDNHKVDCVKRCPLCFVMISPRELYTVYIENVKQCSVGDPIEFVLLTRKKDSFAPTRKNE 328

Query: 1115 QGMC---SVEEVHDSFSKFTFTSDVDLSVREAISELDNWLAKADSDLVDDLSMLPYVCAA 945
                      E++D FSKFTFT DVDLSVR+A+ ELD+W+A+AD +LV+DL    +V AA
Sbjct: 329  HDSAVPNGENEIYDPFSKFTFTQDVDLSVRQAVLELDSWIARADPELVEDLEKHLFVNAA 388

Query: 944  REQLEQRKTSWNEHQIGCGNGDDSAGNISGHTRSP--------LAKNLLSGILSASVNDK 789
             E+LEQRK  WNEH++   +   +       + SP         A +   G   +  ND+
Sbjct: 389  LERLEQRKMYWNEHKLSYYSKLSTTARNQTQSFSPPDVSRVGYQAPSWAHGATDSGSNDQ 448

Query: 788  PTCVEGSSHNPKEGE--SCMAQVDDVIRSPEVCGGALSSSYNDDKSVQMHANGFRDRKDN 615
                E SS +  +GE  S + +  D     +      SSS N+ K    H N  +D KDN
Sbjct: 449  DKSAEDSSVDKSDGESQSSLEKSCDNGHPLDDVDAPSSSSCNESKGFLSHQNDTKDLKDN 508

Query: 614  DSYNFYQAVDGQHLILHPINLKCLLHHYGSYDRLPHRIHGKILQLESVTQSEAMRRRYRY 435
            D+YNFYQ+VDGQH+ILHP+NLKCL+HHYGSYD LP R+ GKIL++E+VTQSEA+RRRYR+
Sbjct: 509  DAYNFYQSVDGQHIILHPLNLKCLMHHYGSYDFLPPRVSGKILEMETVTQSEAIRRRYRF 568

Query: 434  LSHFSLTTTFQLCEIDLSDSLPFDALSPFIDEIKNREKQRKRTARKEQEEKIKAEVAAAT 255
            LSHFSLTTTFQ+CEID+ +SLP DA +PFIDEIK REKQRK  ARK+++ KIKAEVAAA 
Sbjct: 569  LSHFSLTTTFQICEIDMRESLPPDAFAPFIDEIKKREKQRKDRARKDRKNKIKAEVAAAA 628

Query: 254  EYLTMPYNFSQ 222
            E + +  +F Q
Sbjct: 629  ELVPLMSSFGQ 639



 Score = 33.9 bits (76), Expect(2) = e-156
 Identities = 19/52 (36%), Positives = 28/52 (53%), Gaps = 4/52 (7%)
 Frame = -2

Query: 166 SNVTRLGYAAAHDSPALKFEEPRGVPKTDFASNSSP----SGTQSFANVISK 23
           S+VTRLG+AA HDSP    E       +   +N++     +   SFA+V S+
Sbjct: 676 SHVTRLGFAAGHDSPNFSKEPTNAQSSSSVVTNATTGARNTNITSFASVTSR 727