BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00001317
         (708 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAO15531.1|AF457875_1 ribosome inactivating protein Euserrati...   200   3e-49
gb|AAO15530.1|AF457874_1 ribosome inactivating protein Euserrati...   184   1e-44
gb|AFK73428.1| type 1 ribosomal inactivating protein [Jatropha c...   121   1e-25
ref|XP_002532190.1| ricin-agglutinin family protein [Ricinus com...   109   6e-22
gb|AFK73427.1| type 1 ribosomal inactivating protein [Jatropha c...   108   8e-22

>gb|AAO15531.1|AF457875_1 ribosome inactivating protein Euserratin 2 precursor [Euphorbia
           serrata]
          Length = 299

 Score =  200 bits (508), Expect = 3e-49
 Identities = 115/206 (55%), Positives = 146/206 (70%), Gaps = 7/206 (3%)
 Frame = +3

Query: 9   KISITLALYALNSYVIGYKSETRSFFFKEAPSAAQRLLFDGTTKKAVQ-VYTTYDKLADR 185
           ++SITLA+  +N YVIGYKS   SFF K+APS A+ LLF GT K  +  V + Y+ L DR
Sbjct: 89  QLSITLAVTVVNVYVIGYKSAGNSFFLKDAPSDAKTLLFQGTNKITLSSVDSNYNNLGDR 148

Query: 186 SKIPLSTGALKNAIGILHGFDGVNVSTAFKESLVVVLQMVAEATRFKFIQQYIEFNLING 365
           SK+ L  G L  +I  L+ F+GV+V+  FKESL+VV+QMVAEA RFKFIQ  IE NL++ 
Sbjct: 149 SKVGLGIGPLSRSIDTLNKFNGVSVNNVFKESLLVVIQMVAEAARFKFIQLKIENNLLDE 208

Query: 366 YTPKGDTISYENAWDPLSEQIQLSDSKGKFKTPVRVQNADYTFKDITSVDQVKSDIAILL 545
           Y PK DTISYEN W+ LSEQIQLS + GKFK PV +  A+ T K +++V QVK DI+ILL
Sbjct: 209 YKPKNDTISYENNWEKLSEQIQLSGTDGKFKKPVTLLYANGTDKIVSTVAQVKPDISILL 268

Query: 546 YKG-SGKTIGDD--GDI---SVVEEW 605
           YKG SG T  ++  G+I    V+EEW
Sbjct: 269 YKGSSGITAAEEKVGEIFAKDVMEEW 294


>gb|AAO15530.1|AF457874_1 ribosome inactivating protein Euserratin 1 precursor [Euphorbia
           serrata]
          Length = 297

 Score =  184 bits (468), Expect = 1e-44
 Identities = 99/191 (51%), Positives = 132/191 (69%), Gaps = 1/191 (0%)
 Frame = +3

Query: 9   KISITLALYALNSYVIGYKSETRSFFFKEAPSAAQRLLFDGTTKKAVQVYTTYDKLADRS 188
           K ++TLAL  LN YVIGYKS T+SFF K+APS A+ LLF  TT K ++V T Y+ L DRS
Sbjct: 88  KRTVTLALTVLNVYVIGYKSGTKSFFLKDAPSDAKTLLFTDTTPKTLEVDTNYNNLGDRS 147

Query: 189 KIPLSTGALKNAIGILHGFDGVNVSTAFKESLVVVLQMVAEATRFKFIQQYIEFNLINGY 368
           K+ L   ALKNAI IL+ FDGV+    FK SL++V+QMV+EA RFKFIQ  IE  L+  Y
Sbjct: 148 KVGLGIPALKNAINILNQFDGVSTDQDFKHSLLIVIQMVSEAARFKFIQLKIEGGLLTQY 207

Query: 369 TPKGDTISYENAWDPLSEQIQLSDSKGKFKTPVRVQNADYTFKDITSVDQVKSDIAILLY 548
            PK DTISY+N W  LS+ IQL+D+ G+    V ++  D   + +  V+QV+ DI++LLY
Sbjct: 208 LPKPDTISYQNNWSALSKSIQLADANGRLSESVTLKYEDGKDRVVFMVEQVQRDISLLLY 267

Query: 549 K-GSGKTIGDD 578
             GS + +G++
Sbjct: 268 HVGSTERLGEE 278


>gb|AFK73428.1| type 1 ribosomal inactivating protein [Jatropha curcas]
          Length = 314

 Score =  121 bits (304), Expect = 1e-25
 Identities = 75/186 (40%), Positives = 107/186 (57%), Gaps = 9/186 (4%)
 Frame = +3

Query: 18  ITLALYALNSYVIGYKSETRSFFFKEAPSA--AQRLLFDGTTKKAVQVYTTYDKL----A 179
           ITL L  +N+YV+ YK    S+FF +      AQ  LF  T +  +++  +YD L     
Sbjct: 104 ITLGLNVINAYVLAYKVGDNSYFFNDPTELKDAQTYLFKDTKQNTIKMTGSYDSLKAQGG 163

Query: 180 DRSKIPLSTGALKNAIGILHGFDGVNVSTAFKE---SLVVVLQMVAEATRFKFIQQYIEF 350
           DR  + +  G L + I  LH       STA K+    LV ++QMV+EA RFK+I++ I  
Sbjct: 164 DRETMDIGIGQLDSHIYTLHK------STALKDIAKPLVCIIQMVSEAARFKYIEKKIID 217

Query: 351 NLINGYTPKGDTISYENAWDPLSEQIQLSDSKGKFKTPVRVQNADYTFKDITSVDQVKSD 530
            +  G+TPK D IS EN W  LSE I+ +D  GKFKT VR+QN D + K I+ VD++  +
Sbjct: 218 EVEGGFTPKLDVISRENNWGGLSEGIENADKNGKFKTTVRLQNEDSSAKVISKVDEIIVE 277

Query: 531 IAILLY 548
           + +LLY
Sbjct: 278 MGVLLY 283


>ref|XP_002532190.1| ricin-agglutinin family protein [Ricinus communis]
           gi|223528122|gb|EEF30193.1| ricin-agglutinin family
           protein [Ricinus communis]
          Length = 303

 Score =  109 bits (272), Expect = 6e-22
 Identities = 68/191 (35%), Positives = 106/191 (55%), Gaps = 5/191 (2%)
 Frame = +3

Query: 21  TLALYALNSYVIGYKSETRSFFFKEAPSAAQ-RLLFDGTTKKAVQVYTTYDKL----ADR 185
           T+AL  +N+YV+G+K   +S+FF +  +      LF  TTK  +    +Y  L    A R
Sbjct: 97  TIALDVINAYVLGFKVGKKSYFFSDVSNEIYGSKLFSDTTKARLPYGGSYQALYNAGASR 156

Query: 186 SKIPLSTGALKNAIGILHGFDGVNVSTAFKESLVVVLQMVAEATRFKFIQQYIEFNLING 365
             +PL      NAI  L  + G   +T    +LVVV+QM++EA RFK+I++ +  N    
Sbjct: 157 ENVPLGISQFNNAIFQLVKYAGAPATTDIASNLVVVIQMISEAARFKYIEEKLVENFYED 216

Query: 366 YTPKGDTISYENAWDPLSEQIQLSDSKGKFKTPVRVQNADYTFKDITSVDQVKSDIAILL 545
             PKGD +S EN W  LSE IQ S + G F  P+R+++ DY    +++V  V++ + +LL
Sbjct: 217 LYPKGDLLSLENNWGTLSEAIQTS-ANGNF-NPIRLEDEDYKPYYVSTVKAVEAKMGLLL 274

Query: 546 YKGSGKTIGDD 578
            K S  +I ++
Sbjct: 275 AKESMLSIDEE 285


>gb|AFK73427.1| type 1 ribosomal inactivating protein [Jatropha curcas]
          Length = 316

 Score =  108 bits (271), Expect = 8e-22
 Identities = 68/185 (36%), Positives = 103/185 (55%), Gaps = 7/185 (3%)
 Frame = +3

Query: 18  ITLALYALNSYVIGYKSETRSFFFKEAPSA--AQRLLFDGTTKKAVQVYT-TYDKL---- 176
           ITL L  +++Y++ YK    S+FFK+      AQ+ LF  T +  +  ++  YD      
Sbjct: 106 ITLGLNVISAYILSYKVGYNSYFFKDKAELKDAQKYLFTDTRQTTLDKFSGNYDSFKAEG 165

Query: 177 ADRSKIPLSTGALKNAIGILHGFDGVNVSTAFKESLVVVLQMVAEATRFKFIQQYIEFNL 356
            DR    L  G L + I  LH      +     + LV ++QMV+EATR+K+I+  I   +
Sbjct: 166 GDRETTDLGIGQLDSHIYTLHKS---TLPKDIAKPLVCIIQMVSEATRYKYIENKIIDKI 222

Query: 357 INGYTPKGDTISYENAWDPLSEQIQLSDSKGKFKTPVRVQNADYTFKDITSVDQVKSDIA 536
              + PK DTI+ EN W+ LS+ I+ +D+KG FKT VR+Q  D     I+SV+Q+  D+ 
Sbjct: 223 SGSFRPKLDTITRENKWEDLSDGIENADAKGNFKTEVRLQKEDGKEDIISSVNQIIGDMG 282

Query: 537 ILLYK 551
           ILLY+
Sbjct: 283 ILLYQ 287