BLASTX nr result
ID: Bupleurum21_contig00001303
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00001303
(1759 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AFG28405.1| glyceraldehyde-3-phosphate dehydrogenase B [Pyrus... 445 e-122
ref|NP_001237135.1| glyceraldehyde-3-phosphate dehydrogenase B s... 440 e-121
emb|CAN69459.1| hypothetical protein VITISV_021576 [Vitis vinifera] 439 e-121
ref|NP_001240080.1| uncharacterized protein LOC100806482 [Glycin... 439 e-120
ref|XP_002307806.1| predicted protein [Populus trichocarpa] gi|2... 438 e-120
>gb|AFG28405.1| glyceraldehyde-3-phosphate dehydrogenase B [Pyrus x bretschneideri]
Length = 452
Score = 445 bits (1145), Expect = e-122
Identities = 244/382 (63%), Positives = 263/382 (68%)
Frame = +1
Query: 151 MACKAALVSSSLPTNTRLPSKTSHSFPSQCFSRRLEVADFSGLRSRVSLSYSKNASEGSF 330
MA AAL SS +P NTRLPSK SHSFP+QCFS+RLEV +FSGLRS ++Y+ N E SF
Sbjct: 1 MASHAALASSRVPANTRLPSKPSHSFPTQCFSKRLEVGEFSGLRSSSCVTYASNGREQSF 60
Query: 331 FDVVASQLSPKTSGLSPVRGETVAKLKVAINGFGRIGRNFLRCWHGRKDSPLEVIVVNDS 510
FD VA+QL+PKT+G +PVRGETVAKLKVAINGFGRIGRNFLRCWHGRKDSPLEVIVVNDS
Sbjct: 61 FDTVAAQLTPKTAGPTPVRGETVAKLKVAINGFGRIGRNFLRCWHGRKDSPLEVIVVNDS 120
Query: 511 GGVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSVDGKNIKVVSNRDPLQLPWAEMG 690
GGV SVDGK +KVVS+RDPL+LPWAEMG
Sbjct: 121 GGV-----KNASHLLKYDSMLGTFKADVKIVDNETISVDGKPVKVVSSRDPLKLPWAEMG 175
Query: 691 IDIVIEGTGVFVDGPGAGKHIQAGAKKVIITAPAKGAXXXXXXXXXXXXXXXXXXXXXXX 870
IDIVIEGTGVFVDGPGAGKHIQAGAKKVIITAPAKGA
Sbjct: 176 IDIVIEGTGVFVDGPGAGKHIQAGAKKVIITAPAKGA----------------DIPTHVV 219
Query: 871 XXXXXXXXXXXANIISNASCTTNCLAPFVKVIDEEFGIVKGTMTTTHSYTGDQXXXXXXX 1050
ANI+SNASCTTNCLAPFVK++DEEFGIVKGTMTTTHSYTGDQ
Sbjct: 220 GVNEKDYGHDVANIVSNASCTTNCLAPFVKILDEEFGIVKGTMTTTHSYTGDQRLLDASH 279
Query: 1051 XXXXXXXXXXXNIVPTSTGAAKAVSLVLPQLKGKLNGIALRXXXXXXXXXXXXXXXEKKG 1230
NIVPTSTGAAKAVSLVLPQLKGKLNGIALR KKG
Sbjct: 280 RDLRRARAAALNIVPTSTGAAKAVSLVLPQLKGKLNGIALRVPTPNVSVVDLVINVAKKG 339
Query: 1231 LTAEDVNAAFRKAADGPLNGIL 1296
++AEDVNAAFRKAADGPL GIL
Sbjct: 340 ISAEDVNAAFRKAADGPLKGIL 361
Score = 117 bits (292), Expect = 1e-23
Identities = 50/60 (83%), Positives = 55/60 (91%)
Frame = +2
Query: 1424 DMVKVIAWYDNEWGYSQRVVDLAHLVASKWPGVVTTGSGDSLEDYCETSPADKECKVYEA 1603
DMVKV+AWYDNEWGYSQRVVDLAHLVASKWPG GSGD LED+C+T+PAD+ECKVYEA
Sbjct: 393 DMVKVVAWYDNEWGYSQRVVDLAHLVASKWPGAAVVGSGDPLEDFCQTNPADEECKVYEA 452
>ref|NP_001237135.1| glyceraldehyde-3-phosphate dehydrogenase B subunit [Glycine max]
gi|77540212|gb|ABA86964.1| glyceraldehyde-3-phosphate
dehydrogenase B subunit [Glycine max]
Length = 451
Score = 440 bits (1132), Expect = e-121
Identities = 240/382 (62%), Positives = 264/382 (69%)
Frame = +1
Query: 151 MACKAALVSSSLPTNTRLPSKTSHSFPSQCFSRRLEVADFSGLRSRVSLSYSKNASEGSF 330
MA AAL S+ +P+NTR PSK SHSFP+QC +RLEV +FSGLRS ++Y+ +A E SF
Sbjct: 1 MATHAALASTRIPSNTRFPSKASHSFPTQCALKRLEVTEFSGLRSTSCVTYANSARESSF 60
Query: 331 FDVVASQLSPKTSGLSPVRGETVAKLKVAINGFGRIGRNFLRCWHGRKDSPLEVIVVNDS 510
FD+VASQL+PKT+G +PVRGETVAKLKVAINGFGRIGRNFLRCWHGRKDSPLEV+VVNDS
Sbjct: 61 FDLVASQLTPKTNGSTPVRGETVAKLKVAINGFGRIGRNFLRCWHGRKDSPLEVVVVNDS 120
Query: 511 GGVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSVDGKNIKVVSNRDPLQLPWAEMG 690
GGV +VDGK IKVVS+RDPL+LPWAE+G
Sbjct: 121 GGV-----KNASHLLKYDSMLGTFKADVKILDNETITVDGKPIKVVSSRDPLKLPWAELG 175
Query: 691 IDIVIEGTGVFVDGPGAGKHIQAGAKKVIITAPAKGAXXXXXXXXXXXXXXXXXXXXXXX 870
IDIVIEGTGVFVDGPGAGKHIQAGAKKVIITAPAKGA
Sbjct: 176 IDIVIEGTGVFVDGPGAGKHIQAGAKKVIITAPAKGA----------------DIPTYVV 219
Query: 871 XXXXXXXXXXXANIISNASCTTNCLAPFVKVIDEEFGIVKGTMTTTHSYTGDQXXXXXXX 1050
+NIISNASCTTNCLAPFVK++DEEFGIVKGTMTTTHSYTGDQ
Sbjct: 220 GVNEGDYTHQISNIISNASCTTNCLAPFVKILDEEFGIVKGTMTTTHSYTGDQRLLDASH 279
Query: 1051 XXXXXXXXXXXNIVPTSTGAAKAVSLVLPQLKGKLNGIALRXXXXXXXXXXXXXXXEKKG 1230
NIVPTSTGAAKAVSLVLPQLKGKLNGIALR EKKG
Sbjct: 280 RDLRRARAAALNIVPTSTGAAKAVSLVLPQLKGKLNGIALRVPTPNVSVVDLVVNVEKKG 339
Query: 1231 LTAEDVNAAFRKAADGPLNGIL 1296
LTAEDVNAAFRKAA+GPL G+L
Sbjct: 340 LTAEDVNAAFRKAAEGPLKGVL 361
Score = 114 bits (286), Expect = 6e-23
Identities = 49/59 (83%), Positives = 54/59 (91%)
Frame = +2
Query: 1424 DMVKVIAWYDNEWGYSQRVVDLAHLVASKWPGVVTTGSGDSLEDYCETSPADKECKVYE 1600
DMVKV+AWYDNEWGYSQRVVDLAHLVASKWPG GSGD LE++CET+PAD+ECKVYE
Sbjct: 393 DMVKVVAWYDNEWGYSQRVVDLAHLVASKWPGAAKAGSGDPLEEFCETNPADEECKVYE 451
>emb|CAN69459.1| hypothetical protein VITISV_021576 [Vitis vinifera]
Length = 453
Score = 439 bits (1130), Expect = e-121
Identities = 245/383 (63%), Positives = 265/383 (69%), Gaps = 1/383 (0%)
Frame = +1
Query: 151 MACKAALVSSSLPT-NTRLPSKTSHSFPSQCFSRRLEVADFSGLRSRVSLSYSKNASEGS 327
MA AAL SS +PT NTRLPSK SHSFP+Q F++RLEVA+FSGLRS ++++KNA E S
Sbjct: 1 MASHAALASSRIPTTNTRLPSKASHSFPTQFFAKRLEVAEFSGLRSGGCVTFAKNAREPS 60
Query: 328 FFDVVASQLSPKTSGLSPVRGETVAKLKVAINGFGRIGRNFLRCWHGRKDSPLEVIVVND 507
FFDVVASQL+PK G +PVRGETVAKLKVAINGFGRIGRNFLRCWHGRKDSPL+VIVVND
Sbjct: 61 FFDVVASQLTPKAGGPAPVRGETVAKLKVAINGFGRIGRNFLRCWHGRKDSPLDVIVVND 120
Query: 508 SGGVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSVDGKNIKVVSNRDPLQLPWAEM 687
SGGV SVDGK IKVVS+RDPL+LPWAE+
Sbjct: 121 SGGV-----KNASHLLKYDSMLGTFKAEVKIVDNETISVDGKPIKVVSSRDPLKLPWAEL 175
Query: 688 GIDIVIEGTGVFVDGPGAGKHIQAGAKKVIITAPAKGAXXXXXXXXXXXXXXXXXXXXXX 867
GIDIVIEGTGVFVDGPGAGKHIQAGAKKVIITAPAKGA
Sbjct: 176 GIDIVIEGTGVFVDGPGAGKHIQAGAKKVIITAPAKGA----------------DIPTYV 219
Query: 868 XXXXXXXXXXXXANIISNASCTTNCLAPFVKVIDEEFGIVKGTMTTTHSYTGDQXXXXXX 1047
+NI+SNASCTTNCLAPFVK++DEE GIVKGTMTTTHSYTGDQ
Sbjct: 220 VGVNEGDYSHEVSNIVSNASCTTNCLAPFVKILDEELGIVKGTMTTTHSYTGDQRLLDAS 279
Query: 1048 XXXXXXXXXXXXNIVPTSTGAAKAVSLVLPQLKGKLNGIALRXXXXXXXXXXXXXXXEKK 1227
NIVPTSTGAAKAVSLVLPQLKGKLNGIALR EKK
Sbjct: 280 HRDLRRARAAALNIVPTSTGAAKAVSLVLPQLKGKLNGIALRVPTPNVSVVDLVVNVEKK 339
Query: 1228 GLTAEDVNAAFRKAADGPLNGIL 1296
G+TAEDVNAAFRKAADGPL GIL
Sbjct: 340 GITAEDVNAAFRKAADGPLKGIL 362
Score = 114 bits (286), Expect = 6e-23
Identities = 49/60 (81%), Positives = 55/60 (91%)
Frame = +2
Query: 1424 DMVKVIAWYDNEWGYSQRVVDLAHLVASKWPGVVTTGSGDSLEDYCETSPADKECKVYEA 1603
DMVKV+AWYDNEWGYSQRVVDLAHLVA+KWPG GSGD LED+C+T+PAD+ECKVYEA
Sbjct: 394 DMVKVVAWYDNEWGYSQRVVDLAHLVAAKWPGFAAEGSGDPLEDFCKTNPADEECKVYEA 453
>ref|NP_001240080.1| uncharacterized protein LOC100806482 [Glycine max]
gi|255636463|gb|ACU18570.1| unknown [Glycine max]
Length = 451
Score = 439 bits (1129), Expect = e-120
Identities = 239/382 (62%), Positives = 264/382 (69%)
Frame = +1
Query: 151 MACKAALVSSSLPTNTRLPSKTSHSFPSQCFSRRLEVADFSGLRSRVSLSYSKNASEGSF 330
MA AAL S+ +P+NTR PSK SHSFP+QC +RLEV +FSGLRS ++Y+ +A E SF
Sbjct: 1 MATHAALASTRIPSNTRFPSKASHSFPTQCALKRLEVTEFSGLRSTSCVTYANSARESSF 60
Query: 331 FDVVASQLSPKTSGLSPVRGETVAKLKVAINGFGRIGRNFLRCWHGRKDSPLEVIVVNDS 510
FD+VASQL+PKT+G +PVRGETVAKLKVAINGFGRIGRNFLRCWHGRKDSPLEV+VVNDS
Sbjct: 61 FDLVASQLTPKTNGSTPVRGETVAKLKVAINGFGRIGRNFLRCWHGRKDSPLEVVVVNDS 120
Query: 511 GGVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSVDGKNIKVVSNRDPLQLPWAEMG 690
GGV +VDGK+IKVVS+RDPL+LPWAE+G
Sbjct: 121 GGV-----KNASHLLKYDSMLGTFKADVKILDNETITVDGKSIKVVSSRDPLKLPWAELG 175
Query: 691 IDIVIEGTGVFVDGPGAGKHIQAGAKKVIITAPAKGAXXXXXXXXXXXXXXXXXXXXXXX 870
IDIVIEGTGVFVDGPGAGKHIQAGAKKVIITAPAKGA
Sbjct: 176 IDIVIEGTGVFVDGPGAGKHIQAGAKKVIITAPAKGA----------------DIPTYVV 219
Query: 871 XXXXXXXXXXXANIISNASCTTNCLAPFVKVIDEEFGIVKGTMTTTHSYTGDQXXXXXXX 1050
+NIISNASCTTNCLAPFVK++D EFGIVKGTMTTTHSYTGDQ
Sbjct: 220 GVNEGDYTHEISNIISNASCTTNCLAPFVKILDAEFGIVKGTMTTTHSYTGDQRLLDASH 279
Query: 1051 XXXXXXXXXXXNIVPTSTGAAKAVSLVLPQLKGKLNGIALRXXXXXXXXXXXXXXXEKKG 1230
NIVPTSTGAAKAVSLVLPQLKGKLNGIALR EKKG
Sbjct: 280 RDLRRARAAALNIVPTSTGAAKAVSLVLPQLKGKLNGIALRVPTPNVSVVDLVVNVEKKG 339
Query: 1231 LTAEDVNAAFRKAADGPLNGIL 1296
LTAEDVNAAFRKAA+GPL G+L
Sbjct: 340 LTAEDVNAAFRKAAEGPLKGVL 361
Score = 115 bits (288), Expect = 4e-23
Identities = 50/59 (84%), Positives = 54/59 (91%)
Frame = +2
Query: 1424 DMVKVIAWYDNEWGYSQRVVDLAHLVASKWPGVVTTGSGDSLEDYCETSPADKECKVYE 1600
DMVKV+AWYDNEWGYSQRVVDLAHLVASKWPG GSGD LED+CET+PAD+ECKVYE
Sbjct: 393 DMVKVVAWYDNEWGYSQRVVDLAHLVASKWPGAPKAGSGDPLEDFCETNPADEECKVYE 451
>ref|XP_002307806.1| predicted protein [Populus trichocarpa] gi|222857255|gb|EEE94802.1|
predicted protein [Populus trichocarpa]
Length = 452
Score = 438 bits (1126), Expect = e-120
Identities = 243/382 (63%), Positives = 262/382 (68%)
Frame = +1
Query: 151 MACKAALVSSSLPTNTRLPSKTSHSFPSQCFSRRLEVADFSGLRSRVSLSYSKNASEGSF 330
MA AAL SS +P NTRLPSK +HSFP+Q +RLEVA+FSGLR+ ++Y+KNASEGSF
Sbjct: 1 MATHAALASSRIPANTRLPSKINHSFPTQSSLKRLEVAEFSGLRASSCVTYAKNASEGSF 60
Query: 331 FDVVASQLSPKTSGLSPVRGETVAKLKVAINGFGRIGRNFLRCWHGRKDSPLEVIVVNDS 510
FDVVASQL+PK + +PVR ETVAKLKVAINGFGRIGRNFLRCWHGRKDSPL+VIVVNDS
Sbjct: 61 FDVVASQLAPKVAVSTPVRAETVAKLKVAINGFGRIGRNFLRCWHGRKDSPLDVIVVNDS 120
Query: 511 GGVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSVDGKNIKVVSNRDPLQLPWAEMG 690
GGV SVDGK IKVVSNRDPL+LPWAE+G
Sbjct: 121 GGV-----KNASHLLKYDSMLGTFKAEVKIVDNETISVDGKPIKVVSNRDPLKLPWAELG 175
Query: 691 IDIVIEGTGVFVDGPGAGKHIQAGAKKVIITAPAKGAXXXXXXXXXXXXXXXXXXXXXXX 870
IDIVIEGTGVFVDGPGAGKHIQAGA KVIITAPAKGA
Sbjct: 176 IDIVIEGTGVFVDGPGAGKHIQAGATKVIITAPAKGA----------------DIPTYVV 219
Query: 871 XXXXXXXXXXXANIISNASCTTNCLAPFVKVIDEEFGIVKGTMTTTHSYTGDQXXXXXXX 1050
ANIISNASCTTNCLAPFVKV+DEEFGIVKGTMTTTHSYTGDQ
Sbjct: 220 GVNEKDYDHEVANIISNASCTTNCLAPFVKVMDEEFGIVKGTMTTTHSYTGDQRLLDASH 279
Query: 1051 XXXXXXXXXXXNIVPTSTGAAKAVSLVLPQLKGKLNGIALRXXXXXXXXXXXXXXXEKKG 1230
NIVPTSTGAAKAVSLVLPQLKGKLNGIALR EKKG
Sbjct: 280 RDLRRARAAALNIVPTSTGAAKAVSLVLPQLKGKLNGIALRVPTPNVSVVDLVVNVEKKG 339
Query: 1231 LTAEDVNAAFRKAADGPLNGIL 1296
+TAEDVN AFRKAA+GPL G+L
Sbjct: 340 ITAEDVNGAFRKAAEGPLKGVL 361
Score = 116 bits (291), Expect = 2e-23
Identities = 49/60 (81%), Positives = 56/60 (93%)
Frame = +2
Query: 1424 DMVKVIAWYDNEWGYSQRVVDLAHLVASKWPGVVTTGSGDSLEDYCETSPADKECKVYEA 1603
DM+KV+AWYDNEWGYSQRVVDLAHLVA+KWPGV GSGD LED+C+T+PAD+ECKVYEA
Sbjct: 393 DMIKVVAWYDNEWGYSQRVVDLAHLVANKWPGVAAAGSGDPLEDFCKTNPADEECKVYEA 452