BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00001262
         (1817 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAS90127.1| NADPH cytochrome P450 reductase [Ammi majus]           758   0.0  
gb|AAB97736.1| NADPH cytochrome P450 reductase [Petroselinum cri...   754   0.0  
gb|AAB97737.1| NADPH cytochrome P450 reductase [Petroselinum cri...   674   0.0  
gb|ACN54324.1| NADPH:cytochrome P450 reductase [Gossypium hirsutum]   672   0.0  
gb|ABI98819.1| cytochrome P450 reductase [Artemisia annua]            666   0.0  

>gb|AAS90127.1| NADPH cytochrome P450 reductase [Ammi majus]
          Length = 681

 Score =  758 bits (1956), Expect = 0.0
 Identities = 376/479 (78%), Positives = 389/479 (81%)
 Frame = +1

Query: 379  RYEKAKFRVVXXXXXXXXXXXXXTKFKKESYVFFFLATYGDGEPTDNAARFYKWFSEGEE 558
            RYEKAKFRV+              KFKKES+ FFFLATYGDGEPTDNAARFYKWFSEGEE
Sbjct: 99   RYEKAKFRVIDLDDYAAEDEEYEEKFKKESFAFFFLATYGDGEPTDNAARFYKWFSEGEE 158

Query: 559  KGEWLNNLQYGVFGLGNRQYEHFNKIAKVVDDGLAEQGAKRIVQVGMGDDDQCIEDDFTA 738
            KGEWLN LQYGVFGLGNRQYEHFNKIAKVVDDGLA+QGAKRIV+VGMGDDDQCIEDDFTA
Sbjct: 159  KGEWLNKLQYGVFGLGNRQYEHFNKIAKVVDDGLADQGAKRIVEVGMGDDDQCIEDDFTA 218

Query: 739  WRELVWPXXXXXXXXXXXXSAATPYTAAVLEYRVVVHDQLDAPTLDRSLSTQNGHAVFDA 918
            WRE VWP            SAATPYTAAVLEYRVVV+DQ D  TLD+SLSTQNGH V DA
Sbjct: 219  WRESVWPELDKLLLDEDDTSAATPYTAAVLEYRVVVYDQPDTATLDQSLSTQNGHTVHDA 278

Query: 919  QHPCRSNVAAKKELHKPASDRSCIHLEFDISHTGLAYETGDHVGVYCXXXXXXXXXXXXX 1098
            QHPCRSNVA KKELHKPASDRSCIHLEFDISHTGLAYETGDHVGVYC             
Sbjct: 279  QHPCRSNVAVKKELHKPASDRSCIHLEFDISHTGLAYETGDHVGVYCENLVEIVEEAEKL 338

Query: 1099 XGIPPTTYFSVHTDNEDXXXXXXXXXXXXXXXXXXRVALAKYADLMXXXXXXXXXXXXXX 1278
             G+PP TYFSVHTDNED                  R ALAKYADL+              
Sbjct: 339  LGMPPNTYFSVHTDNEDGTPLTGGSLPPPFPPCTVRSALAKYADLLSSPKKSALLALAAH 398

Query: 1279 XXXXXEAERLRFLASPAGKDEYAQWVVASHRSLLEVLAEFPSAKPPLGVFFASVAPRLQP 1458
                 EA+RLRFLASPAGKDEYAQW+VASHRSLLEVLAEFPSAKPPLGVFFASVAPRLQP
Sbjct: 399  ASDPTEADRLRFLASPAGKDEYAQWIVASHRSLLEVLAEFPSAKPPLGVFFASVAPRLQP 458

Query: 1459 RYYSISSSPRMVPSRIHVTCALVYEKTPTGRIHKGVCSTWMKNAVSLEESHDCSWAPIFV 1638
            RYYS+SSSPRMVPSRIHVTCALVYEKTPTGRIHKGVCSTWMKNAVSLEESHDCSWAPIFV
Sbjct: 459  RYYSVSSSPRMVPSRIHVTCALVYEKTPTGRIHKGVCSTWMKNAVSLEESHDCSWAPIFV 518

Query: 1639 RQSNFKLPSDSKVPIIMIGPGTGLAPFRGFLQERLALKEAGTELGTAVLYFGCRNRNLD 1815
            RQSNFKLPSD+KVPIIMIGPGTGLAPFRGFLQER ALK+AG ELGTAVLYFGCRNRNLD
Sbjct: 519  RQSNFKLPSDTKVPIIMIGPGTGLAPFRGFLQERQALKDAGAELGTAVLYFGCRNRNLD 577



 Score = 85.1 bits (209), Expect = 6e-14
 Identities = 49/76 (64%), Positives = 54/76 (71%)
 Frame = +2

Query: 5   MAGESSDTSLPASAMIXXXXXXXXXXXTTSIAVLIGCAVVLFWRRSSNQQSAKSPEPPKL 184
           MAGES  TSLPA+ +            TTSIAVLIGCAVVL WRRSS  +S KS EPPKL
Sbjct: 1   MAGESLATSLPATLL---ENRDLLMLLTTSIAVLIGCAVVLVWRRSS-LRSVKSVEPPKL 56

Query: 185 VVPKFDAEDEVDDGKK 232
           +VPK + EDEVDDGKK
Sbjct: 57  IVPKVEIEDEVDDGKK 72


>gb|AAB97736.1| NADPH cytochrome P450 reductase [Petroselinum crispum]
          Length = 681

 Score =  754 bits (1948), Expect = 0.0
 Identities = 377/479 (78%), Positives = 389/479 (81%)
 Frame = +1

Query: 379  RYEKAKFRVVXXXXXXXXXXXXXTKFKKESYVFFFLATYGDGEPTDNAARFYKWFSEGEE 558
            RYEKAKFRVV              KFKKES+ FFFLATYGDGEPTDNAARFYKWFSEGEE
Sbjct: 99   RYEKAKFRVVDLDDYAAEDEEYEAKFKKESFAFFFLATYGDGEPTDNAARFYKWFSEGEE 158

Query: 559  KGEWLNNLQYGVFGLGNRQYEHFNKIAKVVDDGLAEQGAKRIVQVGMGDDDQCIEDDFTA 738
            KG+WLN LQYGVFGLGNRQYEHFNKIAKVVDDGLA+QGAKRIV+VGMGDDDQCIEDDFTA
Sbjct: 159  KGDWLNKLQYGVFGLGNRQYEHFNKIAKVVDDGLADQGAKRIVEVGMGDDDQCIEDDFTA 218

Query: 739  WRELVWPXXXXXXXXXXXXSAATPYTAAVLEYRVVVHDQLDAPTLDRSLSTQNGHAVFDA 918
            WRELVWP            SAATPYTAAVLEYRVVV+DQLD  TLDRSLSTQNGH V DA
Sbjct: 219  WRELVWPELDKLLLDEDDTSAATPYTAAVLEYRVVVYDQLDTATLDRSLSTQNGHTVHDA 278

Query: 919  QHPCRSNVAAKKELHKPASDRSCIHLEFDISHTGLAYETGDHVGVYCXXXXXXXXXXXXX 1098
            QHPCRS+VAAKKELHKPASDRSCIHLEFDISHTGLAYETGDHVGVYC             
Sbjct: 279  QHPCRSSVAAKKELHKPASDRSCIHLEFDISHTGLAYETGDHVGVYCENLVEIVEEAEKL 338

Query: 1099 XGIPPTTYFSVHTDNEDXXXXXXXXXXXXXXXXXXRVALAKYADLMXXXXXXXXXXXXXX 1278
             G+ P TYFSVH D+ED                  R ALAKYADL+              
Sbjct: 339  LGMQPNTYFSVHIDDEDGTPLTGGSLPPPFPPCTVRSALAKYADLLSSPKKSALLALAAH 398

Query: 1279 XXXXXEAERLRFLASPAGKDEYAQWVVASHRSLLEVLAEFPSAKPPLGVFFASVAPRLQP 1458
                 EA+RLR LASPAGKDEYAQWVVASHRSLLEVLAEFPSAKPPLGVFFASVAPRLQP
Sbjct: 399  ASDPTEADRLRLLASPAGKDEYAQWVVASHRSLLEVLAEFPSAKPPLGVFFASVAPRLQP 458

Query: 1459 RYYSISSSPRMVPSRIHVTCALVYEKTPTGRIHKGVCSTWMKNAVSLEESHDCSWAPIFV 1638
            RYYSISSSPRMVPSRIHVTCALVYEKTPTGRIHKGVCSTWMKNAVSLEESHDCSWAPIFV
Sbjct: 459  RYYSISSSPRMVPSRIHVTCALVYEKTPTGRIHKGVCSTWMKNAVSLEESHDCSWAPIFV 518

Query: 1639 RQSNFKLPSDSKVPIIMIGPGTGLAPFRGFLQERLALKEAGTELGTAVLYFGCRNRNLD 1815
            RQSNFKLPSD+KVPIIMIGPGTGLAPFRGFLQER ALK+AG ELGTAVLYFGCRNRNLD
Sbjct: 519  RQSNFKLPSDTKVPIIMIGPGTGLAPFRGFLQERQALKDAGAELGTAVLYFGCRNRNLD 577



 Score = 83.6 bits (205), Expect = 2e-13
 Identities = 48/76 (63%), Positives = 53/76 (69%)
 Frame = +2

Query: 5   MAGESSDTSLPASAMIXXXXXXXXXXXTTSIAVLIGCAVVLFWRRSSNQQSAKSPEPPKL 184
           M GES  TSLPA+ +            TTSIAVLIGCAVVL WRRSS  +S KS EPPKL
Sbjct: 1   MGGESLATSLPATLL---ENRDLLMLLTTSIAVLIGCAVVLVWRRSS-LRSVKSVEPPKL 56

Query: 185 VVPKFDAEDEVDDGKK 232
           +VPK + EDEVDDGKK
Sbjct: 57  IVPKVEIEDEVDDGKK 72


>gb|AAB97737.1| NADPH cytochrome P450 reductase [Petroselinum crispum]
          Length = 699

 Score =  674 bits (1739), Expect = 0.0
 Identities = 337/480 (70%), Positives = 366/480 (76%), Gaps = 1/480 (0%)
 Frame = +1

Query: 379  RYEKAKFRVVXXXXXXXXXXXXXTKFKKESYVFFFLATYGDGEPTDNAARFYKWFSEGEE 558
            RY+ A F+ +             TK KKES VFFFLATYGDGEPTDNAARFYKWF EG+E
Sbjct: 116  RYQDAIFKTIDLDDYAGDDDEYETKLKKESMVFFFLATYGDGEPTDNAARFYKWFCEGKE 175

Query: 559  KGEWLNNLQYGVFGLGNRQYEHFNKIAKVVDDGLAEQGAKRIVQVGMGDDDQCIEDDFTA 738
            +GEWLNNLQYGVFGLGNRQYEHFNKIA VVDDGL EQGAKR+V VGMGDDDQCIEDDFTA
Sbjct: 176  RGEWLNNLQYGVFGLGNRQYEHFNKIAVVVDDGLVEQGAKRLVPVGMGDDDQCIEDDFTA 235

Query: 739  WRELVWPXXXXXXXXXXXXSAATPYTAAVLEYRVVVHDQLDAPT-LDRSLSTQNGHAVFD 915
            WRELVWP            +AATPYTAAVLEYRV  ++Q D  + L RS+S  NGHAV+D
Sbjct: 236  WRELVWPELDQLLLDEESKAAATPYTAAVLEYRVQFYNQTDTSSPLVRSMSKLNGHAVYD 295

Query: 916  AQHPCRSNVAAKKELHKPASDRSCIHLEFDISHTGLAYETGDHVGVYCXXXXXXXXXXXX 1095
            AQHPCR+NVA ++ELH PASDRSC HLEFDIS TGLAYETGDHVGVY             
Sbjct: 296  AQHPCRANVAVRRELHTPASDRSCTHLEFDISSTGLAYETGDHVGVYTENLIEIVEEAER 355

Query: 1096 XXGIPPTTYFSVHTDNEDXXXXXXXXXXXXXXXXXXRVALAKYADLMXXXXXXXXXXXXX 1275
               I P TYFS+HT+NED                  R AL +YADL+             
Sbjct: 356  LIDISPDTYFSIHTENEDGTPLSGGSLPPPFPPCSFRTALTRYADLLSTPKKSALVALAA 415

Query: 1276 XXXXXXEAERLRFLASPAGKDEYAQWVVASHRSLLEVLAEFPSAKPPLGVFFASVAPRLQ 1455
                  EAERLRFLASP GKDEYAQW+VAS RSLLEVLA FPSAKPPLGVFFASVAPRLQ
Sbjct: 416  HASDPSEAERLRFLASPVGKDEYAQWLVASQRSLLEVLAAFPSAKPPLGVFFASVAPRLQ 475

Query: 1456 PRYYSISSSPRMVPSRIHVTCALVYEKTPTGRIHKGVCSTWMKNAVSLEESHDCSWAPIF 1635
            PRYYSISSSPRM PSRIHVTCALV+E TP GRIHKG+CSTWMKNAVSLE++H  SWAPIF
Sbjct: 476  PRYYSISSSPRMAPSRIHVTCALVHETTPAGRIHKGLCSTWMKNAVSLEDAHVSSWAPIF 535

Query: 1636 VRQSNFKLPSDSKVPIIMIGPGTGLAPFRGFLQERLALKEAGTELGTAVLYFGCRNRNLD 1815
            VRQSNF+LP+DSKVPIIMIGPGTGLAPFRGF+QERLALKE+G ELG+AVLYFGCRNR LD
Sbjct: 536  VRQSNFRLPTDSKVPIIMIGPGTGLAPFRGFMQERLALKESGAELGSAVLYFGCRNRKLD 595



 Score = 64.3 bits (155), Expect = 1e-07
 Identities = 31/50 (62%), Positives = 39/50 (78%), Gaps = 1/50 (2%)
 Frame = +2

Query: 86  TTSIAVLIGCAVVLFWRRSSNQ-QSAKSPEPPKLVVPKFDAEDEVDDGKK 232
           TTS AVLIG  +V+ WRRS+   +SAK  EP K+V+PKF+ E+EVDDGKK
Sbjct: 40  TTSFAVLIGLGLVMMWRRSTTMTKSAKKLEPAKIVIPKFEMEEEVDDGKK 89


>gb|ACN54324.1| NADPH:cytochrome P450 reductase [Gossypium hirsutum]
          Length = 710

 Score =  672 bits (1734), Expect = 0.0
 Identities = 327/479 (68%), Positives = 364/479 (75%)
 Frame = +1

Query: 379  RYEKAKFRVVXXXXXXXXXXXXXTKFKKESYVFFFLATYGDGEPTDNAARFYKWFSEGEE 558
            RYEKA F +V              K KK++  FFFLATYGDGEPTDNAARFYKWF+EG+E
Sbjct: 128  RYEKATFNIVDLDDYAADDEEYEEKMKKDNLAFFFLATYGDGEPTDNAARFYKWFTEGKE 187

Query: 559  KGEWLNNLQYGVFGLGNRQYEHFNKIAKVVDDGLAEQGAKRIVQVGMGDDDQCIEDDFTA 738
            +GEWL N++YG+FGLGN+QYEHFNK+AKVVD+ L EQGAKRIV +G+GDDDQCIEDDFTA
Sbjct: 188  RGEWLQNMKYGIFGLGNKQYEHFNKVAKVVDELLTEQGAKRIVPLGLGDDDQCIEDDFTA 247

Query: 739  WRELVWPXXXXXXXXXXXXSAATPYTAAVLEYRVVVHDQLDAPTLDRSLSTQNGHAVFDA 918
            WRELVWP            + +TPYTAAVLEYRVV +D  DAP  D++ S  NGHA +DA
Sbjct: 248  WRELVWPELDQLLRDEDDATVSTPYTAAVLEYRVVFYDPADAPLEDKNWSNANGHATYDA 307

Query: 919  QHPCRSNVAAKKELHKPASDRSCIHLEFDISHTGLAYETGDHVGVYCXXXXXXXXXXXXX 1098
            QHPCRSNVA +KELH P SDRSC HLEFDI+ TGL+YETGDHVGVYC             
Sbjct: 308  QHPCRSNVAVRKELHAPESDRSCTHLEFDIAGTGLSYETGDHVGVYCENLDEVVDEALSL 367

Query: 1099 XGIPPTTYFSVHTDNEDXXXXXXXXXXXXXXXXXXRVALAKYADLMXXXXXXXXXXXXXX 1278
             G+ P TYFSVHTD ED                  R ALA+YADL+              
Sbjct: 368  LGLSPDTYFSVHTDKEDGTPLGGSSLPSSFPPCTLRTALARYADLLSSPKKAALLALAAH 427

Query: 1279 XXXXXEAERLRFLASPAGKDEYAQWVVASHRSLLEVLAEFPSAKPPLGVFFASVAPRLQP 1458
                 EA+RLR LASPAGKDEYAQW+VA+ RSLLEV+AEFPSAKPPLGVFFA+VAPRLQP
Sbjct: 428  ASDPTEADRLRHLASPAGKDEYAQWIVANQRSLLEVMAEFPSAKPPLGVFFAAVAPRLQP 487

Query: 1459 RYYSISSSPRMVPSRIHVTCALVYEKTPTGRIHKGVCSTWMKNAVSLEESHDCSWAPIFV 1638
            RYYSISSSPR+ PSRIHVTCALVYEKTPTGRIHKGVCSTWMKNAVS  +S DC WAPIFV
Sbjct: 488  RYYSISSSPRLAPSRIHVTCALVYEKTPTGRIHKGVCSTWMKNAVSSGKSDDCGWAPIFV 547

Query: 1639 RQSNFKLPSDSKVPIIMIGPGTGLAPFRGFLQERLALKEAGTELGTAVLYFGCRNRNLD 1815
            RQSNFKLPSD+KVPIIMIGPGTGLAPFRGFLQERLALKEAG ELG +VL+FGCRNR +D
Sbjct: 548  RQSNFKLPSDTKVPIIMIGPGTGLAPFRGFLQERLALKEAGAELGPSVLFFGCRNRKMD 606


>gb|ABI98819.1| cytochrome P450 reductase [Artemisia annua]
          Length = 704

 Score =  666 bits (1718), Expect = 0.0
 Identities = 331/479 (69%), Positives = 361/479 (75%)
 Frame = +1

Query: 379  RYEKAKFRVVXXXXXXXXXXXXXTKFKKESYVFFFLATYGDGEPTDNAARFYKWFSEGEE 558
            RYEKA F+V+              K KKES  FFFLATYGDGEPTDNAARFYKWF+EGEE
Sbjct: 125  RYEKAVFKVIDLDDYAAEDDEYEEKLKKESLAFFFLATYGDGEPTDNAARFYKWFTEGEE 184

Query: 559  KGEWLNNLQYGVFGLGNRQYEHFNKIAKVVDDGLAEQGAKRIVQVGMGDDDQCIEDDFTA 738
            KGEWL+ LQY VFGLGNRQYEHFNKIAKVVD+ L EQGAKR+V VGMGDDDQCIEDDFTA
Sbjct: 185  KGEWLDKLQYAVFGLGNRQYEHFNKIAKVVDEKLVEQGAKRLVPVGMGDDDQCIEDDFTA 244

Query: 739  WRELVWPXXXXXXXXXXXXSAATPYTAAVLEYRVVVHDQLDAPTLDRSLSTQNGHAVFDA 918
            W+ELVWP            S ATPYTAAV EYRVV HD+ +  T D+   T NGHAV DA
Sbjct: 245  WKELVWPELDQLLRDEDDTSVATPYTAAVAEYRVVFHDKPE--TYDQDQLT-NGHAVHDA 301

Query: 919  QHPCRSNVAAKKELHKPASDRSCIHLEFDISHTGLAYETGDHVGVYCXXXXXXXXXXXXX 1098
            QHPCRSNVA KKELH P SDRSC HLEFDIS+TGL+YETGDHVGVY              
Sbjct: 302  QHPCRSNVAVKKELHSPLSDRSCTHLEFDISNTGLSYETGDHVGVYVENLSEVVDEAEKL 361

Query: 1099 XGIPPTTYFSVHTDNEDXXXXXXXXXXXXXXXXXXRVALAKYADLMXXXXXXXXXXXXXX 1278
             G+PP TYFSVH DNED                  R ALA YAD++              
Sbjct: 362  IGLPPHTYFSVHADNEDGTPLGGASLPPPFPPCTLRKALASYADVLSSPKKSALLALAAH 421

Query: 1279 XXXXXEAERLRFLASPAGKDEYAQWVVASHRSLLEVLAEFPSAKPPLGVFFASVAPRLQP 1458
                 EA+RL+FLASPAGKDEYAQW+VASHRSLLEV+  FPSAKPPLGVFFASVAPRLQP
Sbjct: 422  ATDSTEADRLKFLASPAGKDEYAQWIVASHRSLLEVMEAFPSAKPPLGVFFASVAPRLQP 481

Query: 1459 RYYSISSSPRMVPSRIHVTCALVYEKTPTGRIHKGVCSTWMKNAVSLEESHDCSWAPIFV 1638
            RYYSISSSPR  P+RIHVTCALVYE+TP+GR+HKGVCSTWMKNAV + ES DCSWAPI+V
Sbjct: 482  RYYSISSSPRFAPNRIHVTCALVYEQTPSGRVHKGVCSTWMKNAVPMTESQDCSWAPIYV 541

Query: 1639 RQSNFKLPSDSKVPIIMIGPGTGLAPFRGFLQERLALKEAGTELGTAVLYFGCRNRNLD 1815
            R SNF+LPSD KVP+IMIGPGTGLAPFRGFLQERLA KEAGTELGTA+L+FGCRNR +D
Sbjct: 542  RTSNFRLPSDPKVPVIMIGPGTGLAPFRGFLQERLAQKEAGTELGTAILFFGCRNRKVD 600



 Score = 64.7 bits (156), Expect = 8e-08
 Identities = 36/72 (50%), Positives = 47/72 (65%)
 Frame = +2

Query: 17  SSDTSLPASAMIXXXXXXXXXXXTTSIAVLIGCAVVLFWRRSSNQQSAKSPEPPKLVVPK 196
           +SDT++P +  +           TTS+AVLIGC VVL WRRSS+  + K+ E P +VVPK
Sbjct: 30  TSDTNIPLAVFMENRELLMIL--TTSVAVLIGCVVVLVWRRSSS-AAKKAAESPVIVVPK 86

Query: 197 FDAEDEVDDGKK 232
              EDEVDDG+K
Sbjct: 87  KVTEDEVDDGRK 98