BLASTX nr result
ID: Bupleurum21_contig00001011
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00001011
(2043 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_003631732.1| PREDICTED: cysteinyl-tRNA synthetase-like [V... 670 0.0
ref|XP_002262997.2| PREDICTED: cysteinyl-tRNA synthetase-like [V... 670 0.0
ref|XP_002315932.1| predicted protein [Populus trichocarpa] gi|2... 641 0.0
ref|XP_004150192.1| PREDICTED: cysteine--tRNA ligase-like [Cucum... 628 0.0
ref|XP_002533372.1| cysteinyl-tRNA synthetase, putative [Ricinus... 622 0.0
>ref|XP_003631732.1| PREDICTED: cysteinyl-tRNA synthetase-like [Vitis vinifera]
gi|296087508|emb|CBI34097.3| unnamed protein product
[Vitis vinifera]
Length = 513
Score = 670 bits (1729), Expect(2) = 0.0
Identities = 333/487 (68%), Positives = 389/487 (79%), Gaps = 1/487 (0%)
Frame = -3
Query: 1933 ELTIYNSLTKQKEVFKPIVPGKVGMYVCGVTAYDYSHIGHARAYVSFDILYRYLRYLGYE 1754
EL +YN++T+QKE+FKP+V GKV MYVCGVTAYD SH+GHARA V FD+LYRYL++LGYE
Sbjct: 6 ELRVYNTMTQQKEIFKPVVDGKVSMYVCGVTAYDLSHLGHARAAVCFDVLYRYLQHLGYE 65
Query: 1753 VMYVRNFTDVDDKIIKRANERGVDPISLSAEFSQEFLKDMADLQCLPPTHQPRVSDHMEQ 1574
V YVRNFTDVDDKII+RANE G +P++LS+ F QE+L DM DLQCL PT QPRVSDHM+Q
Sbjct: 66 VTYVRNFTDVDDKIIRRANELGENPLTLSSRFCQEYLDDMTDLQCLTPTQQPRVSDHMDQ 125
Query: 1573 IINMISQIENNGCAYRVEGDVYFSVDNSPKYGQLSGRRLEDNRSGERVAVDSRKRNPADF 1394
I +MI+QI NN CAY ++GDVYF+VD SP YG+LSGR+LEDNR+GERVAVDSRKRNPADF
Sbjct: 126 IKDMITQIINNDCAYIIDGDVYFAVDKSPNYGRLSGRKLEDNRAGERVAVDSRKRNPADF 185
Query: 1393 ALWKAAKDGEPSWESPWGPGRPGWHIECSAMSAEYLTPTFDIHGGGMDLIFPHHENEIAQ 1214
ALWKAAK EPSW+SPWGPGRPGWHIECSAMSA YLT FDIHGGG+DLIFPHHENEIAQ
Sbjct: 186 ALWKAAKPDEPSWDSPWGPGRPGWHIECSAMSAHYLTFKFDIHGGGIDLIFPHHENEIAQ 245
Query: 1213 SSAACPESNVSYWMHNGFVNTNDEKMSKSLGNFFTIRKVTEMYHPLALRHFLLGTHYRSP 1034
S AAC ESNVSYWMHNG V N+EKMSKSLGNFFTIR++T++YHPLALR+FLLGTHYRSP
Sbjct: 246 SCAACQESNVSYWMHNGHVTNNNEKMSKSLGNFFTIRQITDLYHPLALRYFLLGTHYRSP 305
Query: 1033 VNYSIAQIEIASEAVYRIYQTLQDCQDALSSLGEGSVNMVAEQKGSRVRITDAAQECIRN 854
VNYSIAQ+EIASEAV+ IYQTL+DC+D LS E G V IT AA++CI
Sbjct: 306 VNYSIAQLEIASEAVFYIYQTLRDCEDTLSPF--------QEVNGQNVCITPAAKDCISK 357
Query: 853 LQSDVK-KMSDDLHTFSLLNSALPEAXXXXXXXXXXXXXXXXXXXXXXXXXSLVELEKEV 677
LQ+D + KMSDDL T +LN++L EA SL ELEKEV
Sbjct: 358 LQTDFQTKMSDDLSTPHILNASLQEALRFINSGLNMLKKKQQKQQQLSAVQSLTELEKEV 417
Query: 676 RNALDILGLLSELNYIEVLSQLKSKALKRSGFEDEYIENQIKERALARKNKEFSRGDKIR 497
+ L +LGLLS L Y EVL QLK KAL+R+G ++ + + I+ERALARKNK FSRGD+IR
Sbjct: 418 KGVLSVLGLLSTLAYSEVLQQLKGKALRRAGMIEDDVLHLIEERALARKNKNFSRGDQIR 477
Query: 496 SDLAAKG 476
+DLA+KG
Sbjct: 478 ADLASKG 484
Score = 43.5 bits (101), Expect(2) = 0.0
Identities = 19/23 (82%), Positives = 19/23 (82%)
Frame = -1
Query: 393 ALMDVGNETIWRPCVPVPQEQPA 325
ALMDVG ETIWRPCVPV QE A
Sbjct: 486 ALMDVGKETIWRPCVPVEQEPQA 508
>ref|XP_002262997.2| PREDICTED: cysteinyl-tRNA synthetase-like [Vitis vinifera]
gi|297735988|emb|CBI23962.3| unnamed protein product
[Vitis vinifera]
Length = 513
Score = 670 bits (1728), Expect(2) = 0.0
Identities = 331/487 (67%), Positives = 390/487 (80%), Gaps = 1/487 (0%)
Frame = -3
Query: 1933 ELTIYNSLTKQKEVFKPIVPGKVGMYVCGVTAYDYSHIGHARAYVSFDILYRYLRYLGYE 1754
EL +YN++T+QKE+ KP+V GKV MYVCGVTAYD SH+GH RA VSFD+LYRYL++LGYE
Sbjct: 6 ELRVYNTMTQQKEILKPVVDGKVSMYVCGVTAYDLSHLGHGRAAVSFDVLYRYLQHLGYE 65
Query: 1753 VMYVRNFTDVDDKIIKRANERGVDPISLSAEFSQEFLKDMADLQCLPPTHQPRVSDHMEQ 1574
V YVRNFTDVDDKII+RANE G +P++LS+ F QE+L DM DL CL PTHQPRVSDHM+Q
Sbjct: 66 VTYVRNFTDVDDKIIRRANELGENPLTLSSHFCQEYLDDMTDLHCLSPTHQPRVSDHMDQ 125
Query: 1573 IINMISQIENNGCAYRVEGDVYFSVDNSPKYGQLSGRRLEDNRSGERVAVDSRKRNPADF 1394
I +MI+QI NN CAY ++GDVYF+VD SP YG+LSGR+LE+NR+GERVAVDSRKRNPADF
Sbjct: 126 IKDMITQIINNDCAYTIDGDVYFAVDKSPNYGRLSGRKLENNRAGERVAVDSRKRNPADF 185
Query: 1393 ALWKAAKDGEPSWESPWGPGRPGWHIECSAMSAEYLTPTFDIHGGGMDLIFPHHENEIAQ 1214
ALWKAAK GEPSW+SPWGPGRPGWHIECSAMSA YLT FDIHGGG+DLIFPHHENEIAQ
Sbjct: 186 ALWKAAKPGEPSWDSPWGPGRPGWHIECSAMSAHYLTFKFDIHGGGIDLIFPHHENEIAQ 245
Query: 1213 SSAACPESNVSYWMHNGFVNTNDEKMSKSLGNFFTIRKVTEMYHPLALRHFLLGTHYRSP 1034
S AAC ESNVSYWMHNG V N+EKMSKSLGNFFTIR++T++YHPLALR+FLLGTHYRSP
Sbjct: 246 SCAACQESNVSYWMHNGHVTNNNEKMSKSLGNFFTIRQITDLYHPLALRYFLLGTHYRSP 305
Query: 1033 VNYSIAQIEIASEAVYRIYQTLQDCQDALSSLGEGSVNMVAEQKGSRVRITDAAQECIRN 854
VNYSIAQ+EIASEAV+ IYQTL+DC+D LS + E G V I AAQ+CIR
Sbjct: 306 VNYSIAQLEIASEAVFYIYQTLRDCEDTLS--------LFQEVNGRNVCIAPAAQDCIRT 357
Query: 853 LQSDVK-KMSDDLHTFSLLNSALPEAXXXXXXXXXXXXXXXXXXXXXXXXXSLVELEKEV 677
LQ+D + KMSDDL T +LN++L EA SL +L KEV
Sbjct: 358 LQTDFQTKMSDDLSTPHILNASLQEALRFINSCLNMLKKKQQKQQQLSAVESLTKLGKEV 417
Query: 676 RNALDILGLLSELNYIEVLSQLKSKALKRSGFEDEYIENQIKERALARKNKEFSRGDKIR 497
+ L +LGLLS L Y EVL QLK KALKR+G ++ + + I+ERALARKNK+FS+GD+IR
Sbjct: 418 KGVLSVLGLLSTLAYSEVLQQLKGKALKRAGMIEDDVLHLIEERALARKNKDFSKGDQIR 477
Query: 496 SDLAAKG 476
+DLA+KG
Sbjct: 478 ADLASKG 484
Score = 43.5 bits (101), Expect(2) = 0.0
Identities = 19/23 (82%), Positives = 19/23 (82%)
Frame = -1
Query: 393 ALMDVGNETIWRPCVPVPQEQPA 325
ALMDVG ETIWRPCVPV QE A
Sbjct: 486 ALMDVGKETIWRPCVPVQQEPQA 508
>ref|XP_002315932.1| predicted protein [Populus trichocarpa] gi|222864972|gb|EEF02103.1|
predicted protein [Populus trichocarpa]
Length = 532
Score = 641 bits (1654), Expect(2) = 0.0
Identities = 315/487 (64%), Positives = 381/487 (78%), Gaps = 1/487 (0%)
Frame = -3
Query: 1933 ELTIYNSLTKQKEVFKPIVPGKVGMYVCGVTAYDYSHIGHARAYVSFDILYRYLRYLGYE 1754
EL +YNS+T+QKEVFK +PGKV MYVCGVT+YD+SH+GHARA V+FDIL+RYL++LGYE
Sbjct: 6 ELKLYNSMTQQKEVFKSRIPGKVSMYVCGVTSYDFSHLGHARAAVAFDILFRYLQHLGYE 65
Query: 1753 VMYVRNFTDVDDKIIKRANERGVDPISLSAEFSQEFLKDMADLQCLPPTHQPRVSDHMEQ 1574
V YVRNFTD+DDKII+RANE G DP+SLS+ F +E+L DM DLQCL PTHQPRV+DH+EQ
Sbjct: 66 VTYVRNFTDIDDKIIRRANEIGEDPLSLSSRFCEEYLVDMTDLQCLIPTHQPRVTDHVEQ 125
Query: 1573 IINMISQIENNGCAYRVEGDVYFSVDNSPKYGQLSGRRLEDNRSGERVAVDSRKRNPADF 1394
I +MI+QI CAY VEGDV+F+V+ SP YGQLSG+RLE+NR+GERVAVDSRKRNPADF
Sbjct: 126 IKDMITQIIEKDCAYAVEGDVFFAVNKSPNYGQLSGQRLENNRAGERVAVDSRKRNPADF 185
Query: 1393 ALWKAAKDGEPSWESPWGPGRPGWHIECSAMSAEYLTPTFDIHGGGMDLIFPHHENEIAQ 1214
ALWKAAK GEPSWESPWGPGRPGWHIECSAMSA+YLT FDIHGGG+DLIFPHHENEIAQ
Sbjct: 186 ALWKAAKPGEPSWESPWGPGRPGWHIECSAMSAQYLTFKFDIHGGGIDLIFPHHENEIAQ 245
Query: 1213 SSAACPESNVSYWMHNGFVNTNDEKMSKSLGNFFTIRKVTEMYHPLALRHFLLGTHYRSP 1034
S AAC ES+VSYWMHNG V N+EKMSKSLGNFFTIR++TE YHPLALRHFL+ HYRSP
Sbjct: 246 SCAACEESSVSYWMHNGHVTNNNEKMSKSLGNFFTIRQITERYHPLALRHFLISAHYRSP 305
Query: 1033 VNYSIAQIEIASEAVYRIYQTLQDCQDALSSLGEGSVNMVAEQKGSRVRITDAAQECIRN 854
+NYS++Q+E +S+AV+ IYQTLQDC+DAL EGS+ A Q + V IT AQ+CI
Sbjct: 306 LNYSVSQLESSSDAVFYIYQTLQDCEDALLPFQEGSLKEGAGQNANLVAITADAQKCISR 365
Query: 853 LQSDVK-KMSDDLHTFSLLNSALPEAXXXXXXXXXXXXXXXXXXXXXXXXXSLVELEKEV 677
L D + KMSDDL+T LL A EA S+ E++KEV
Sbjct: 366 LHEDFETKMSDDLNTSPLLTGAFQEALKVVNGSLGMLKKKQQKKQQLSLIRSVTEVKKEV 425
Query: 676 RNALDILGLLSELNYIEVLSQLKSKALKRSGFEDEYIENQIKERALARKNKEFSRGDKIR 497
L ILGL EVL QLK KALKR+G ++ + + I++RA+ARK+++F + D+IR
Sbjct: 426 TEVLRILGLFPPCTCAEVLQQLKGKALKRAGLTEDDVMSLIEDRAVARKSQDFKKSDQIR 485
Query: 496 SDLAAKG 476
+DL+A+G
Sbjct: 486 TDLSARG 492
Score = 42.4 bits (98), Expect(2) = 0.0
Identities = 17/23 (73%), Positives = 19/23 (82%)
Frame = -1
Query: 393 ALMDVGNETIWRPCVPVPQEQPA 325
ALMDVG ET+WRPCVPV E+ A
Sbjct: 494 ALMDVGKETVWRPCVPVENEEKA 516
>ref|XP_004150192.1| PREDICTED: cysteine--tRNA ligase-like [Cucumis sativus]
gi|449508099|ref|XP_004163218.1| PREDICTED:
cysteine--tRNA ligase-like [Cucumis sativus]
Length = 562
Score = 628 bits (1620), Expect(2) = 0.0
Identities = 307/489 (62%), Positives = 375/489 (76%), Gaps = 1/489 (0%)
Frame = -3
Query: 1939 KGELTIYNSLTKQKEVFKPIVPGKVGMYVCGVTAYDYSHIGHARAYVSFDILYRYLRYLG 1760
K E +YNS+T+QKEVF PGKVGMYVCG+TAYD+SHIGHARA V+FD+LYRYL++LG
Sbjct: 3 KEEFRVYNSMTQQKEVFTTKEPGKVGMYVCGITAYDFSHIGHARAAVNFDVLYRYLKHLG 62
Query: 1759 YEVMYVRNFTDVDDKIIKRANERGVDPISLSAEFSQEFLKDMADLQCLPPTHQPRVSDHM 1580
YEV YVRNFTDVDDKII+RANE G +P +LS F QE+L DMADLQCL PTHQPRVSDH+
Sbjct: 63 YEVTYVRNFTDVDDKIIRRANESGENPFALSDRFCQEYLSDMADLQCLSPTHQPRVSDHL 122
Query: 1579 EQIINMISQIENNGCAYRVEGDVYFSVDNSPKYGQLSGRRLEDNRSGERVAVDSRKRNPA 1400
EQI +MI+QI NG Y V+GDV+FSVD P YGQLSG++LE++R+GERVAVDSRK NPA
Sbjct: 123 EQIKDMITQIIKNGYGYAVDGDVFFSVDKFPNYGQLSGQKLENHRAGERVAVDSRKNNPA 182
Query: 1399 DFALWKAAKDGEPSWESPWGPGRPGWHIECSAMSAEYLTPTFDIHGGGMDLIFPHHENEI 1220
DFALWKAAK GEPSWESPWGPGRPGWHIECSAMSA YLT FDIHGGG+DLIFPHHENE+
Sbjct: 183 DFALWKAAKPGEPSWESPWGPGRPGWHIECSAMSAHYLTFKFDIHGGGIDLIFPHHENEV 242
Query: 1219 AQSSAACPESNVSYWMHNGFVNTNDEKMSKSLGNFFTIRKVTEMYHPLALRHFLLGTHYR 1040
AQS AAC ES +SYWMHNG V N+EKMSKSLGNFFTIR++TE YHPL LRHFL+ HYR
Sbjct: 243 AQSCAACQESKISYWMHNGHVTNNNEKMSKSLGNFFTIRQITERYHPLVLRHFLISAHYR 302
Query: 1039 SPVNYSIAQIEIASEAVYRIYQTLQDCQDALSSLGEGSVNMVAEQKGSRVRITDAAQECI 860
SP+NY+++Q++ AS+ VY IYQT+QDC+DALS +++ + G + ++ AA+ECI
Sbjct: 303 SPLNYTVSQLDSASDTVYYIYQTMQDCEDALSQHQGENLSDGLGKTGKKDPVSSAAEECI 362
Query: 859 RNLQSDVK-KMSDDLHTFSLLNSALPEAXXXXXXXXXXXXXXXXXXXXXXXXXSLVELEK 683
NL+S+ + +M+DDL+T +L A EA SLV ++K
Sbjct: 363 INLRSEFQTRMADDLNTAHILTGAFQEALKFINSTLTSLKKKQPKKQQLSMIQSLVNVKK 422
Query: 682 EVRNALDILGLLSELNYIEVLSQLKSKALKRSGFEDEYIENQIKERALARKNKEFSRGDK 503
E+R LD+LGLLS Y EVL QLK KALKR+G ++ I + I+ER ARK+K F + D
Sbjct: 423 ELREVLDVLGLLSTSTYSEVLQQLKDKALKRAGMVEDDILHLIEERTQARKDKNFGKSDN 482
Query: 502 IRSDLAAKG 476
IR+ L++ G
Sbjct: 483 IRAKLSSLG 491
Score = 48.5 bits (114), Expect(2) = 0.0
Identities = 26/51 (50%), Positives = 34/51 (66%)
Frame = -1
Query: 393 ALMDVGNETIWRPCVPVPQEQPAVLKPCADASKQEQSQLPPVKQEEPVMPS 241
ALMDVG ETIWRPCVPV +PAVL P + K Q++ P++Q+ P+
Sbjct: 493 ALMDVGRETIWRPCVPV---EPAVLVPPGE-QKATQAEQKPMEQQVAQPPA 539
>ref|XP_002533372.1| cysteinyl-tRNA synthetase, putative [Ricinus communis]
gi|223526794|gb|EEF29017.1| cysteinyl-tRNA synthetase,
putative [Ricinus communis]
Length = 530
Score = 622 bits (1605), Expect(2) = 0.0
Identities = 308/489 (62%), Positives = 373/489 (76%), Gaps = 1/489 (0%)
Frame = -3
Query: 1939 KGELTIYNSLTKQKEVFKPIVPGKVGMYVCGVTAYDYSHIGHARAYVSFDILYRYLRYLG 1760
K E +YN++ +Q E FKP VPGKVGMYVCGVTAY +SHIGHARA V FD+L+RYL++LG
Sbjct: 4 KVEFKLYNTMNRQLEAFKPHVPGKVGMYVCGVTAYAFSHIGHARAAVIFDVLFRYLQHLG 63
Query: 1759 YEVMYVRNFTDVDDKIIKRANERGVDPISLSAEFSQEFLKDMADLQCLPPTHQPRVSDHM 1580
YEV Y RNFTD+DDKIIKRANE G DP SLS+ F +E+L DM LQCL PTHQPRV+DH+
Sbjct: 64 YEVTYARNFTDIDDKIIKRANESGEDPFSLSSRFCEEYLVDMDSLQCLRPTHQPRVTDHI 123
Query: 1579 EQIINMISQIENNGCAYRVEGDVYFSVDNSPKYGQLSGRRLEDNRSGERVAVDSRKRNPA 1400
EQI +MI+QI NNG AY EGDV+F+VD P YGQLSG++LE++R+GER D+RKRNPA
Sbjct: 124 EQIKDMITQIINNGFAYPSEGDVFFAVDKFPNYGQLSGQKLENHRAGERNP-DTRKRNPA 182
Query: 1399 DFALWKAAKDGEPSWESPWGPGRPGWHIECSAMSAEYLTPTFDIHGGGMDLIFPHHENEI 1220
DFALWKAAK GEPSWESPWGPGRPGWHIECSAMSA+YLT FDIHGGG DLIFPHHENEI
Sbjct: 183 DFALWKAAKPGEPSWESPWGPGRPGWHIECSAMSAQYLTFKFDIHGGGSDLIFPHHENEI 242
Query: 1219 AQSSAACPESNVSYWMHNGFVNTNDEKMSKSLGNFFTIRKVTEMYHPLALRHFLLGTHYR 1040
QS+AAC ESNVSYW+HNG V N+EKMSKSLGN+FTIR+VTE YHPLA+RHFL+ HYR
Sbjct: 243 TQSAAACQESNVSYWIHNGHVTNNNEKMSKSLGNYFTIREVTERYHPLAVRHFLISAHYR 302
Query: 1039 SPVNYSIAQIEIASEAVYRIYQTLQDCQDALSSLGEGSVNMVAEQKGSRVRITDAAQECI 860
SP+NY++ Q+E AS+A++ IYQTLQDC+DALS +GS+ A Q V IT A++CI
Sbjct: 303 SPLNYTVFQLESASDAIFYIYQTLQDCEDALSMFQDGSLKEGAAQNSKVVGITADAKKCI 362
Query: 859 RNLQSDVK-KMSDDLHTFSLLNSALPEAXXXXXXXXXXXXXXXXXXXXXXXXXSLVELEK 683
L+ + + KMSDDL T +L A +A SL E+E+
Sbjct: 363 NKLRDEFETKMSDDLSTSHILTGAFQDALKFINSSISMLKKKLQKPQQLSLIQSLTEIER 422
Query: 682 EVRNALDILGLLSELNYIEVLSQLKSKALKRSGFEDEYIENQIKERALARKNKEFSRGDK 503
E++ L+ILGLL Y E+L QLK KALKR+G ++ + I+ERA ARKNKEFSR D+
Sbjct: 423 EIKLVLNILGLLPPGTYAEILQQLKDKALKRAGVTEDDVLRSIEERAQARKNKEFSRSDQ 482
Query: 502 IRSDLAAKG 476
+R++LAAKG
Sbjct: 483 VRANLAAKG 491
Score = 47.0 bits (110), Expect(2) = 0.0
Identities = 20/24 (83%), Positives = 21/24 (87%)
Frame = -1
Query: 393 ALMDVGNETIWRPCVPVPQEQPAV 322
ALMDVG ET+WRPCVPV QEQ AV
Sbjct: 493 ALMDVGKETVWRPCVPVEQEQEAV 516