BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00000478
         (1894 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002279139.1| PREDICTED: acyl-CoA synthetase family member...   798   0.0  
ref|XP_002298229.1| predicted protein [Populus trichocarpa] gi|2...   786   0.0  
gb|AFD33354.1| acyl-activating enzyme 10 [Cannabis sativa]            780   0.0  
ref|XP_003530154.1| PREDICTED: acyl-CoA synthetase family member...   758   0.0  
ref|XP_003531671.1| PREDICTED: acyl-CoA synthetase family member...   758   0.0  

>ref|XP_002279139.1| PREDICTED: acyl-CoA synthetase family member 3, mitochondrial-like
            [Vitis vinifera]
          Length = 552

 Score =  798 bits (2062), Expect = 0.0
 Identities = 408/556 (73%), Positives = 469/556 (84%), Gaps = 14/556 (2%)
 Frame = -3

Query: 1850 MDVFKAVGREGHTSGDRVAIRADNKSYSYFQLVSSACRISHLLFT-----SYG--KTVDA 1692
            M+V KAV R+G  + + VAIRA+ KSYSY QL+SSA +IS LL       +YG  K   +
Sbjct: 1    MEVIKAVARQGSATAESVAIRANQKSYSYNQLISSARKISSLLCNGDIKPTYGVSKHKHS 60

Query: 1691 TAEHLGGARIGIVAKPSAEFVAGVLATWFSGGVAVPLALSYPEAELLHVMNDSDISMVLS 1512
               HLGGARIGIVAKPS EFVAG+L TWFSGGVAVPLALSYPEAELLHVMNDSD+SM+LS
Sbjct: 61   GNGHLGGARIGIVAKPSVEFVAGILGTWFSGGVAVPLALSYPEAELLHVMNDSDVSMILS 120

Query: 1511 TEDHQELMKNIAAKKAAHFSLIPPVPS--SQTNG-DVSQVGKLDANASLERIENSNKIEG 1341
            TED++ELM+N+AAK +A FSLIPPVPS  S T+  D  Q G++ A+ SL+      +I+ 
Sbjct: 121  TEDYRELMENVAAKSSAQFSLIPPVPSIPSPTSARDHPQTGEIVADKSLQ-----GEID- 174

Query: 1340 EDPALILYTSGTTGKPKGVVHTHKSILAQVQILSKAWEYTYEDQFLNCLPLHHVHGLFNA 1161
            EDPALI+YTSGTTGKPKGVVHTHKSI +QVQIL++AW YT  DQFL+CLPLHHVHGLFNA
Sbjct: 175  EDPALIIYTSGTTGKPKGVVHTHKSINSQVQILTEAWGYTSADQFLHCLPLHHVHGLFNA 234

Query: 1160 LFAPLYAGSVVEFMPKFSVRGVWQRWRESYPKDGTKVNEAITVFTGVPTIYSRLIQGYEA 981
            L APLYAGS VEFMPKFSVRG+WQRWRES+PKDGTKV++AITVFTGVPT+Y+RLIQGYEA
Sbjct: 235  LLAPLYAGSTVEFMPKFSVRGIWQRWRESHPKDGTKVDDAITVFTGVPTMYTRLIQGYEA 294

Query: 980  MDPEGKVTSASAARQLRLMMSGSSALPLPVMQQWEAITGHRLLERYGMTEFVMGISNPLK 801
            MDPE +  SASAA +LRLMM GSSALP PVMQQWE ITGHRLLERYGMTEFVM ISNPLK
Sbjct: 295  MDPELQAASASAASKLRLMMCGSSALPYPVMQQWETITGHRLLERYGMTEFVMAISNPLK 354

Query: 800  GLRKGGTVGFPLPGVQTKIVAEDD----SAVGELCIKSPSLFKEYWNLPEVTRSSFTDDM 633
            G+RKGGTVG P PGVQ KI+A++     + VGELCIKSPSLFKEYW LPEVT+ SF D  
Sbjct: 355  GVRKGGTVGKPFPGVQVKILADESETETTGVGELCIKSPSLFKEYWKLPEVTKESFIDGG 414

Query: 632  FFKTGDAATVDEDGYFVILGRNNADIMKVGGYKLSALEIEATLLDHPSVSECCVLGLMDK 453
            FFKTGDA  VD+DGY++ILGR +ADIMKVGGYKLSALEIEA LL+HP+VSECCVLGL DK
Sbjct: 415  FFKTGDAVKVDDDGYYIILGRTSADIMKVGGYKLSALEIEAALLEHPAVSECCVLGLPDK 474

Query: 452  TYGEIVCAIVVPEAEVKRKREEKMMPALSLEELSTWAKEKLAPYKIPTRLILWDSIPRNA 273
             YGE VCAI+VPEA+ K+K+EE++ PA+SLEEL TWAKEKLAPYK+PT+L+LWD +PRNA
Sbjct: 475  DYGEAVCAIIVPEADEKKKQEEELKPAISLEELCTWAKEKLAPYKLPTQLLLWDLLPRNA 534

Query: 272  MGKVNKKELKNILAAQ 225
            MGKVNKKELK  LAA+
Sbjct: 535  MGKVNKKELKRKLAAE 550


>ref|XP_002298229.1| predicted protein [Populus trichocarpa] gi|222845487|gb|EEE83034.1|
            predicted protein [Populus trichocarpa]
          Length = 539

 Score =  786 bits (2030), Expect = 0.0
 Identities = 398/551 (72%), Positives = 457/551 (82%), Gaps = 9/551 (1%)
 Frame = -3

Query: 1850 MDVFKAVGREGHTSGDRVAIRADNKSYSYFQLVSSACRISHLLFTSYGKTVDATA--EHL 1677
            M+V K V R+G  + D +AIRA++KSYSY QL+SSA  IS LL  S  +TVD     +HL
Sbjct: 1    MEVVKKVYRQGTAAADSIAIRAEHKSYSYKQLISSAWEISDLLCNSGLETVDGVGGCKHL 60

Query: 1676 GGARIGIVAKPSAEFVAGVLATWFSGGVAVPLALSYPEAELLHVMNDSDISMVLSTEDHQ 1497
            GGARIGIVAKPSAEFVAG+LATWFSGGVAVPLALSYPE ELLHVMND++ISMVLSTEDHQ
Sbjct: 61   GGARIGIVAKPSAEFVAGILATWFSGGVAVPLALSYPETELLHVMNDAEISMVLSTEDHQ 120

Query: 1496 ELMKNIAAKKAAHFSLIPPV---PSSQTNGDVSQVGKLDANASLERIENSNKIEGEDPAL 1326
            ELM+N+A K AA FSLIP V    S ++  D SQV ++    +LE+        G+DPAL
Sbjct: 121  ELMQNVADKCAAQFSLIPSVHRISSHESVHDHSQVEEI----ALEK--------GDDPAL 168

Query: 1325 ILYTSGTTGKPKGVVHTHKSILAQVQILSKAWEYTYEDQFLNCLPLHHVHGLFNALFAPL 1146
            I+YTSGTTGKPKG VHTHKSI AQVQ L++AWEY+  DQFLNCLPLHHVHG FN+LFAP+
Sbjct: 169  IIYTSGTTGKPKGAVHTHKSITAQVQTLAEAWEYSSTDQFLNCLPLHHVHGFFNSLFAPI 228

Query: 1145 YAGSVVEFMPKFSVRGVWQRWRESYPKDGTKVNEAITVFTGVPTIYSRLIQGYEAMDPEG 966
            YAGS VEFMPKFSVRG+WQRWRESYP +G+K ++AITVFTGVPTIYSRLIQGY AMD + 
Sbjct: 229  YAGSTVEFMPKFSVRGIWQRWRESYPPNGSKADDAITVFTGVPTIYSRLIQGYGAMDSDQ 288

Query: 965  KVTSASAARQLRLMMSGSSALPLPVMQQWEAITGHRLLERYGMTEFVMGISNPLKGLRKG 786
            +  SASAARQLRLMMSGSSALPLPVMQ+WEAITGHRLLERYGMTEFVM ISNPL+G+RK 
Sbjct: 289  QAASASAARQLRLMMSGSSALPLPVMQEWEAITGHRLLERYGMTEFVMAISNPLRGVRKA 348

Query: 785  GTVGFPLPGVQTKIVAE----DDSAVGELCIKSPSLFKEYWNLPEVTRSSFTDDMFFKTG 618
            GTVG P PGVQ KI  +    D + VGELCI+SPS+FKEYW LP+VT  SFTDD FF+TG
Sbjct: 349  GTVGKPFPGVQVKIAEDQNENDTTGVGELCIRSPSMFKEYWKLPQVTEDSFTDDGFFRTG 408

Query: 617  DAATVDEDGYFVILGRNNADIMKVGGYKLSALEIEATLLDHPSVSECCVLGLMDKTYGEI 438
            DA  +DEDGY+VILGR +ADIMKVGGYKLSALEIE+ LL+HP V+ECCVLGL DKTYG+ 
Sbjct: 409  DAGKIDEDGYYVILGRTSADIMKVGGYKLSALEIESALLEHPVVAECCVLGLPDKTYGDA 468

Query: 437  VCAIVVPEAEVKRKREEKMMPALSLEELSTWAKEKLAPYKIPTRLILWDSIPRNAMGKVN 258
            VCAI+VPE  VKRK+EE   PA+SLEEL  WAK+KLAPYK+PTRL LW+S+P NAMGKVN
Sbjct: 469  VCAIIVPEDAVKRKQEESSKPAISLEELREWAKDKLAPYKLPTRLFLWESLPHNAMGKVN 528

Query: 257  KKELKNILAAQ 225
            KKELK +LAA+
Sbjct: 529  KKELKKVLAAE 539


>gb|AFD33354.1| acyl-activating enzyme 10 [Cannabis sativa]
          Length = 564

 Score =  780 bits (2015), Expect = 0.0
 Identities = 398/563 (70%), Positives = 454/563 (80%), Gaps = 20/563 (3%)
 Frame = -3

Query: 1850 MDVFKAVGREGHTSGDRVAIRADNKSYSYFQLVSSACRISHLLFTSYGKTVDATAE---- 1683
            M+V K V +EG  + + VAIRAD KSYSY QL+SSA  I  LL  +  K +    +    
Sbjct: 1    MEVLKEVAKEGSAAREGVAIRADQKSYSYKQLISSAQSICSLLCGTELKAIHEAGKQARP 60

Query: 1682 -----------HLGGARIGIVAKPSAEFVAGVLATWFSGGVAVPLALSYPEAELLHVMND 1536
                       HLGGARIGIVAKPSAEFVAGVL TW SGGVAVPLALSYPEAELLHVMND
Sbjct: 61   SASINGAGGHGHLGGARIGIVAKPSAEFVAGVLGTWLSGGVAVPLALSYPEAELLHVMND 120

Query: 1535 SDISMVLSTEDHQELMKNIAAKKAAHFSLIPPVPSSQTNGDVSQVGKLDANASLERIENS 1356
            SDISM+LSTEDHQELM+NIAAK +A FSLIP VPSS +  +V+   +   + S + I  +
Sbjct: 121  SDISMILSTEDHQELMQNIAAKTSAQFSLIPSVPSSCSQ-EVAVDHRQTGDISTDSILLN 179

Query: 1355 NKIEGEDPALILYTSGTTGKPKGVVHTHKSILAQVQILSKAWEYTYEDQFLNCLPLHHVH 1176
             +I  E+PALI+YTSGTTGKPKGVVHTH+SI AQVQ L+KAWEYT  DQFL+CLPLHHVH
Sbjct: 180  REISNENPALIVYTSGTTGKPKGVVHTHQSISAQVQTLAKAWEYTPADQFLHCLPLHHVH 239

Query: 1175 GLFNALFAPLYAGSVVEFMPKFSVRGVWQRWRESYPKDGTKVNEAITVFTGVPTIYSRLI 996
            GLFNALFAPLYA S VEF+PKFSVRG+WQRWRESYP   TK N+ ITVFTGVPT+Y+RLI
Sbjct: 240  GLFNALFAPLYARSTVEFLPKFSVRGIWQRWRESYPTSETKANDCITVFTGVPTMYTRLI 299

Query: 995  QGYEAMDPEGKVTSASAARQLRLMMSGSSALPLPVMQQWEAITGHRLLERYGMTEFVMGI 816
            QGYEAMDPE K  SASAA+QLRLMM GSSALP+PVMQQW+ ITGHRLLERYGMTEFVM I
Sbjct: 300  QGYEAMDPELKEASASAAKQLRLMMCGSSALPVPVMQQWQTITGHRLLERYGMTEFVMAI 359

Query: 815  SNPLKGLRKGGTVGFPLPGVQTKIVAEDD-----SAVGELCIKSPSLFKEYWNLPEVTRS 651
            SNPLKG RK GTVG P PGV+ +I+AED+     + VGELC++SPSLFKEYW LPEVT++
Sbjct: 360  SNPLKGERKSGTVGKPFPGVEVRILAEDENGDDATGVGELCVRSPSLFKEYWRLPEVTKA 419

Query: 650  SFTDDMFFKTGDAATVDEDGYFVILGRNNADIMKVGGYKLSALEIEATLLDHPSVSECCV 471
            SFTDD FFKTGDA  VDEDGY+VILGR +ADIMKVGGYKLSALEIE+ LL+HP+V ECCV
Sbjct: 420  SFTDDGFFKTGDAGKVDEDGYYVILGRTSADIMKVGGYKLSALEIESVLLEHPTVEECCV 479

Query: 470  LGLMDKTYGEIVCAIVVPEAEVKRKREEKMMPALSLEELSTWAKEKLAPYKIPTRLILWD 291
            LGL DK YGE V AI+VP AE K+KREE+  PA+SLEEL +WA+ KLAPYK+PTRL LWD
Sbjct: 480  LGLPDKDYGEAVSAIIVPAAEAKKKREEESRPAISLEELFSWAQHKLAPYKLPTRLFLWD 539

Query: 290  SIPRNAMGKVNKKELKNILAAQE 222
            S+PRNAMGKVNKKELK  L  ++
Sbjct: 540  SLPRNAMGKVNKKELKKKLTVEQ 562


>ref|XP_003530154.1| PREDICTED: acyl-CoA synthetase family member 3, mitochondrial-like
            [Glycine max]
          Length = 606

 Score =  758 bits (1958), Expect = 0.0
 Identities = 382/560 (68%), Positives = 454/560 (81%), Gaps = 9/560 (1%)
 Frame = -3

Query: 1874 SVSDYSKLMDVFKAVGREGHTSGDRVAIRADNKSYSYFQLVSSACRISHLLFTSYGKTVD 1695
            S S  + LM+V KA+ +    + + VAIRAD KSYSY QL++SA +IS+LL  S     D
Sbjct: 56   SSSAPATLMEVVKAIAKHEPAAPESVAIRADQKSYSYKQLITSAQKISNLLCGS-----D 110

Query: 1694 ATAEHLGGARIGIVAKPSAEFVAGVLATWFSGGVAVPLALSYPEAELLHVMNDSDISMVL 1515
            A   +LGGARIGIVAKPSAEFVAG+L  W SGGVAVPLA SYPE ELL+V+N+SD+S +L
Sbjct: 111  AQTGNLGGARIGIVAKPSAEFVAGILGIWLSGGVAVPLATSYPEVELLYVINNSDVSAIL 170

Query: 1514 STEDHQELMKNIAAKKAAHFSLIPPV---PSSQTNGDVSQVGKLDANASL-ERIENSNKI 1347
            STEDH E+M+++A K ++ F  +PPV    S ++    SQ G +  +  L ++   S+  
Sbjct: 171  STEDHTEIMQSVANKSSSQFFHLPPVLNKSSEKSRDKHSQNGGIHTDKILLDKFGRSS-- 228

Query: 1346 EGEDPALILYTSGTTGKPKGVVHTHKSILAQVQILSKAWEYTYEDQFLNCLPLHHVHGLF 1167
              EDPALILYTSGTTGKPKGVVHTH+SI++QVQ L+KAWEY+  DQFL+CLPLHHVHGLF
Sbjct: 229  --EDPALILYTSGTTGKPKGVVHTHRSIISQVQTLTKAWEYSSADQFLHCLPLHHVHGLF 286

Query: 1166 NALFAPLYAGSVVEFMPKFSVRGVWQRWRESYPKDGTKVNEAITVFTGVPTIYSRLIQGY 987
            N L APLYAGS VEF+PKFSVRGVWQRWRESYP DG+K  EAITVFTGVPTIY+RLIQGY
Sbjct: 287  NGLMAPLYAGSTVEFLPKFSVRGVWQRWRESYPTDGSKAEEAITVFTGVPTIYARLIQGY 346

Query: 986  EAMDPEGKVTSASAARQLRLMMSGSSALPLPVMQQWEAITGHRLLERYGMTEFVMGISNP 807
             AMDPE +  S SAA+ LRLMM GSSALPLPVMQ+WEAITGHRLLERYGMTEFVM +SNP
Sbjct: 347  HAMDPELQAASVSAAKNLRLMMCGSSALPLPVMQEWEAITGHRLLERYGMTEFVMALSNP 406

Query: 806  LKGLRKGGTVGFPLPGVQTKIVAEDDS-----AVGELCIKSPSLFKEYWNLPEVTRSSFT 642
            LKG RK GTVG P PG+Q KI+A+++S      +GELCIKSPSLFKEYW LPEVT+ SFT
Sbjct: 407  LKGERKPGTVGKPFPGIQVKIIADEESVNGNTGMGELCIKSPSLFKEYWKLPEVTKESFT 466

Query: 641  DDMFFKTGDAATVDEDGYFVILGRNNADIMKVGGYKLSALEIEATLLDHPSVSECCVLGL 462
            DD FFKTGDA T DEDGYF+ILGR NADI+K GGYKLSALEIE+ +++HP+VSECCVLGL
Sbjct: 467  DDGFFKTGDAVTTDEDGYFIILGRTNADIIKAGGYKLSALEIESVIIEHPAVSECCVLGL 526

Query: 461  MDKTYGEIVCAIVVPEAEVKRKREEKMMPALSLEELSTWAKEKLAPYKIPTRLILWDSIP 282
             DK YGEIV AIVVPEA+VKRK++++  P LSLEELS WAK+K+APYKIPT+LI+WD +P
Sbjct: 527  PDKDYGEIVSAIVVPEADVKRKQDQESKPVLSLEELSNWAKDKIAPYKIPTQLIVWDKLP 586

Query: 281  RNAMGKVNKKELKNILAAQE 222
            RNAMGKVNKKELK +L +++
Sbjct: 587  RNAMGKVNKKELKKLLVSEQ 606


>ref|XP_003531671.1| PREDICTED: acyl-CoA synthetase family member 3, mitochondrial-like
            [Glycine max]
          Length = 601

 Score =  758 bits (1956), Expect = 0.0
 Identities = 385/561 (68%), Positives = 451/561 (80%), Gaps = 8/561 (1%)
 Frame = -3

Query: 1892 SRFFSDSVSDYSKLMDVFKAVGREGHTSGDRVAIRADNKSYSYFQLVSSACRISHLLFTS 1713
            S+F   S S  + LM+V KA+ +   T  + VAIRAD KSYSY QLVSSA +IS+LL  S
Sbjct: 47   SQFRPLSSSAPATLMEVVKAIAKHEPTVPESVAIRADQKSYSYKQLVSSAQKISNLLCGS 106

Query: 1712 YGKTVDATAEHLGGARIGIVAKPSAEFVAGVLATWFSGGVAVPLALSYPEAELLHVMNDS 1533
                 DA   +LGGAR+GIVAKPSAEFVAG+L  W SGGVAVPLA SYPE ELL+V N+S
Sbjct: 107  -----DAQTGNLGGARVGIVAKPSAEFVAGILGIWLSGGVAVPLATSYPEVELLYVTNNS 161

Query: 1532 DISMVLSTEDHQELMKNIAAKKAAHFSLIPPV---PSSQTNGDVSQVGKLDANASLERIE 1362
            D+S +LSTEDH E+M++IA K ++ F  +P V    S ++  D SQ G +  +  L  ++
Sbjct: 162  DVSAILSTEDHSEIMQSIANKSSSQFFHLPLVLNKSSEKSRDDHSQNGGIHTDKIL--LD 219

Query: 1361 NSNKIEGEDPALILYTSGTTGKPKGVVHTHKSILAQVQILSKAWEYTYEDQFLNCLPLHH 1182
            N  ++  EDPALILYTSGTTGKPKGVVHTHKSI++QVQ L+KAWEYT  DQFL+CLPLHH
Sbjct: 220  NFGRLS-EDPALILYTSGTTGKPKGVVHTHKSIISQVQTLTKAWEYTSADQFLHCLPLHH 278

Query: 1181 VHGLFNALFAPLYAGSVVEFMPKFSVRGVWQRWRESYPKDGTKVNEAITVFTGVPTIYSR 1002
            VHG FN L APLYAGS VEF+PKFSVRGVWQRWRESYP DG+K  +AITVFTGVPTIY+R
Sbjct: 279  VHGFFNGLMAPLYAGSTVEFLPKFSVRGVWQRWRESYPTDGSKAEDAITVFTGVPTIYAR 338

Query: 1001 LIQGYEAMDPEGKVTSASAARQLRLMMSGSSALPLPVMQQWEAITGHRLLERYGMTEFVM 822
            LIQGY AMDPE +  S SAA+ LRLMM GSSALPLPVMQ+WEAITGHRLLERYGMTEFVM
Sbjct: 339  LIQGYHAMDPELQAASVSAAKNLRLMMCGSSALPLPVMQEWEAITGHRLLERYGMTEFVM 398

Query: 821  GISNPLKGLRKGGTVGFPLPGVQTKIVAEDDS-----AVGELCIKSPSLFKEYWNLPEVT 657
             +SNPLKG RK GTVG P PG+Q KI+ +++S      +GELC KSPSLFKEYW LPE T
Sbjct: 399  ALSNPLKGERKPGTVGKPFPGIQVKIITDEESVNENTGMGELCFKSPSLFKEYWKLPEAT 458

Query: 656  RSSFTDDMFFKTGDAATVDEDGYFVILGRNNADIMKVGGYKLSALEIEATLLDHPSVSEC 477
            + SFTDD FFKTGDA T DEDGYF+ILGRNNADI+K GGYKLSALEIE+ +++HP+VSEC
Sbjct: 459  KESFTDDGFFKTGDAVTTDEDGYFIILGRNNADIIKAGGYKLSALEIESVIIEHPAVSEC 518

Query: 476  CVLGLMDKTYGEIVCAIVVPEAEVKRKREEKMMPALSLEELSTWAKEKLAPYKIPTRLIL 297
            CVLGL DK YGEIV AIVVP+A+VK KR+++  P LSLEELSTWAK+K+APYKIPT+LI+
Sbjct: 519  CVLGLPDKDYGEIVGAIVVPQADVKLKRDQESKPVLSLEELSTWAKDKIAPYKIPTQLIV 578

Query: 296  WDSIPRNAMGKVNKKELKNIL 234
            WD +PRNAMGKVNKKELK +L
Sbjct: 579  WDKLPRNAMGKVNKKELKKLL 599