BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00000473
         (1851 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CAB66003.1| NADP-dependent malate dehydrogenase (decarboxyla...   719   0.0  
sp|P34105.3|MAOX_POPTR RecName: Full=NADP-dependent malic enzyme...   675   0.0  
gb|AAK83073.1|AF288920_1 putative cytosolic NADP-malic enzyme [F...   675   0.0  
prf||1803524A malic enzyme                                            675   0.0  
ref|XP_002324450.1| predicted protein [Populus trichocarpa] gi|2...   675   0.0  

>emb|CAB66003.1| NADP-dependent malate dehydrogenase (decarboxylating) [Apium
            graveolens]
          Length = 570

 Score =  719 bits (1856), Expect = 0.0
 Identities = 361/414 (87%), Positives = 374/414 (90%)
 Frame = +2

Query: 608  MATVAGGVQDVYGEDCATEDQLVTPWTISVASGYTLLRNPHHNKGLAFTEKERDAHYLRG 787
            MAT  GG QDVYGEDCA EDQLVTPWTISVASGYTLLR+PHHNKGLAFTEKERDAHYLRG
Sbjct: 1    MAT--GGDQDVYGEDCAAEDQLVTPWTISVASGYTLLRDPHHNKGLAFTEKERDAHYLRG 58

Query: 788  LLPPAVMSQQLQEKKLMQSLRSYDVPLHRYTALMELQERNERLFYKLLIDNVEELLPVVY 967
            LLPPA++SQQ QEKKLMQ+LRSY+VPLHRY A+MELQERNERLFYKLLIDNVEELLPVVY
Sbjct: 59   LLPPAIISQQQQEKKLMQNLRSYEVPLHRYMAMMELQERNERLFYKLLIDNVEELLPVVY 118

Query: 968  TPTVGEACQKYGSIFKRPQGLYISXXXXXXXXXXXXNWPEKDIQVIVVTDGERILGLGDL 1147
            TPTVGEACQKYGSIF+RPQGLYIS            NWPEK+IQVIVVTDGERILGLGDL
Sbjct: 119  TPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPEKNIQVIVVTDGERILGLGDL 178

Query: 1148 GCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNQSLLDNEFYIGLKQRRATGKEYA 1327
            GCQGMGIPVGKL+LYTALGGVRPSACLPITIDVGTNN+SLL+NEFYIGLKQRRATGKEYA
Sbjct: 179  GCQGMGIPVGKLALYTALGGVRPSACLPITIDVGTNNESLLNNEFYIGLKQRRATGKEYA 238

Query: 1328 DLLHEFMAAVKQNYGEKVLVQFEDFANHNAFELLSKYSTTHLVFNDDIQGTAXXXXXXXX 1507
            DLL EFM AVKQNYGEKVLVQFEDFANHNAFELLSKYSTTHLVFNDDIQGTA        
Sbjct: 239  DLLQEFMTAVKQNYGEKVLVQFEDFANHNAFELLSKYSTTHLVFNDDIQGTASVVLSGIV 298

Query: 1508 XXXXXXXXTLADHTFLFLGAGEAGTGIAELIALEMSKQTNISLEETRKKIWLVDSKGLIV 1687
                     LADHTFLFLGAGEAGTGIAELIALEMSKQT I LEETRKKIWLVDSKGLIV
Sbjct: 299  SSLKLIGGMLADHTFLFLGAGEAGTGIAELIALEMSKQTKIHLEETRKKIWLVDSKGLIV 358

Query: 1688 SHRKESLQHFKKPWAHEHEPCTTLIEAVKAIKPTALIGTSGQGKTFTKEVIEAM 1849
            SHRKESLQHFKKPWAHEHEPCTTLI+AVKAIKPT LIGTSGQGKTFTKEV+EAM
Sbjct: 359  SHRKESLQHFKKPWAHEHEPCTTLIDAVKAIKPTVLIGTSGQGKTFTKEVVEAM 412


>sp|P34105.3|MAOX_POPTR RecName: Full=NADP-dependent malic enzyme; Short=NADP-ME
            gi|20469|emb|CAA39690.1| malic enzyme [Populus
            trichocarpa]
          Length = 591

 Score =  675 bits (1742), Expect = 0.0
 Identities = 339/431 (78%), Positives = 369/431 (85%), Gaps = 7/431 (1%)
 Frame = +2

Query: 578  SILKER----SLIEM---ATVAGGVQDVYGEDCATEDQLVTPWTISVASGYTLLRNPHHN 736
            S LKE     S+++M   +TV GGV+DVYGED ATEDQLVTPWTISVASGYTLLR+PHHN
Sbjct: 3    STLKEMRDGASVLDMDPKSTVGGGVEDVYGEDRATEDQLVTPWTISVASGYTLLRDPHHN 62

Query: 737  KGLAFTEKERDAHYLRGLLPPAVMSQQLQEKKLMQSLRSYDVPLHRYTALMELQERNERL 916
            KGLAFTEKERDAHYLRGLLPP  +SQQLQEKKLM ++R Y +PL +YTA+MEL+ERNERL
Sbjct: 63   KGLAFTEKERDAHYLRGLLPPTTISQQLQEKKLMNTIRQYQLPLQKYTAMMELEERNERL 122

Query: 917  FYKLLIDNVEELLPVVYTPTVGEACQKYGSIFKRPQGLYISXXXXXXXXXXXXNWPEKDI 1096
            FYKLLIDNVEELLPVVYTPTVGEACQKYGSIFKRPQGLYIS            NWP+K I
Sbjct: 123  FYKLLIDNVEELLPVVYTPTVGEACQKYGSIFKRPQGLYISLKEKGKVLDVLKNWPQKSI 182

Query: 1097 QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNQSLLDN 1276
            QVIVVTDGERILGLGDLGCQG+GIPVGKLSLYTALGGVRPSACLP+TIDVGTNN+ LL +
Sbjct: 183  QVIVVTDGERILGLGDLGCQGIGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEQLLKD 242

Query: 1277 EFYIGLKQRRATGKEYADLLHEFMAAVKQNYGEKVLVQFEDFANHNAFELLSKYSTTHLV 1456
            EFYIGL+QRRATG+EY++LLHEFM AVKQNYGEKVL+QFEDFANHNAF+LL+KY TTHLV
Sbjct: 243  EFYIGLRQRRATGQEYSELLHEFMTAVKQNYGEKVLIQFEDFANHNAFDLLAKYGTTHLV 302

Query: 1457 FNDDIQGTAXXXXXXXXXXXXXXXXTLADHTFLFLGAGEAGTGIAELIALEMSKQTNISL 1636
            FNDDIQGTA                +LADHTFLFLGAGEAGTGIAELIALEMS+++   L
Sbjct: 303  FNDDIQGTAAVVLAGLISALKLLGGSLADHTFLFLGAGEAGTGIAELIALEMSRRSKTPL 362

Query: 1637 EETRKKIWLVDSKGLIVSHRKESLQHFKKPWAHEHEPCTTLIEAVKAIKPTALIGTSGQG 1816
            EETRKKIWL DSKGLIVS RKESLQHFKKPWAHEHEP   L+E VKAIKP  LIGTSG G
Sbjct: 363  EETRKKIWLTDSKGLIVSSRKESLQHFKKPWAHEHEPVKGLLEVVKAIKPIVLIGTSGVG 422

Query: 1817 KTFTKEVIEAM 1849
            KTFTKEVIEAM
Sbjct: 423  KTFTKEVIEAM 433


>gb|AAK83073.1|AF288920_1 putative cytosolic NADP-malic enzyme [Flaveria pringlei]
          Length = 589

 Score =  675 bits (1742), Expect = 0.0
 Identities = 335/429 (78%), Positives = 376/429 (87%), Gaps = 5/429 (1%)
 Frame = +2

Query: 578  SILKE----RSLIEMAT-VAGGVQDVYGEDCATEDQLVTPWTISVASGYTLLRNPHHNKG 742
            SILK+     S+++ ++ V GGV DVYGED A+EDQL+TPWT+SVASGYTLLR+PHHNKG
Sbjct: 3    SILKQMKEGESVVDPSSAVGGGVDDVYGEDRASEDQLITPWTVSVASGYTLLRDPHHNKG 62

Query: 743  LAFTEKERDAHYLRGLLPPAVMSQQLQEKKLMQSLRSYDVPLHRYTALMELQERNERLFY 922
            LAFTE+ERD+HYLRGLLPPAV +Q+LQEKKLMQ++RSY+VPLHRY A+MEL+ERNERLFY
Sbjct: 63   LAFTERERDSHYLRGLLPPAVATQELQEKKLMQNIRSYEVPLHRYVAMMELEERNERLFY 122

Query: 923  KLLIDNVEELLPVVYTPTVGEACQKYGSIFKRPQGLYISXXXXXXXXXXXXNWPEKDIQV 1102
            KLLIDNVEELLPVVYTPTVGEACQKYGSIFKRPQGLYIS            NWPE++IQV
Sbjct: 123  KLLIDNVEELLPVVYTPTVGEACQKYGSIFKRPQGLYISLKEKGKILEVLRNWPERNIQV 182

Query: 1103 IVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNQSLLDNEF 1282
            IVVTDGERILGLGDLGCQGMGIPVGKL+LYTALGGVRPSACLPITIDVGTNNQ LLD+EF
Sbjct: 183  IVVTDGERILGLGDLGCQGMGIPVGKLALYTALGGVRPSACLPITIDVGTNNQKLLDDEF 242

Query: 1283 YIGLKQRRATGKEYADLLHEFMAAVKQNYGEKVLVQFEDFANHNAFELLSKYSTTHLVFN 1462
            YIGL+Q+RATGKEY DLL EFM+AVKQNYGEKVLVQFEDFANHNAFELL+KY T+HLVFN
Sbjct: 243  YIGLRQKRATGKEYYDLLEEFMSAVKQNYGEKVLVQFEDFANHNAFELLAKYRTSHLVFN 302

Query: 1463 DDIQGTAXXXXXXXXXXXXXXXXTLADHTFLFLGAGEAGTGIAELIALEMSKQTNISLEE 1642
            DDIQGTA                +LADHTFLFLGAGEAGTGIAELIALE+S + NI ++E
Sbjct: 303  DDIQGTASVVLAGLVASLKLLGGSLADHTFLFLGAGEAGTGIAELIALEISTKANIPIDE 362

Query: 1643 TRKKIWLVDSKGLIVSHRKESLQHFKKPWAHEHEPCTTLIEAVKAIKPTALIGTSGQGKT 1822
             RKKIWLVDSKGLIVS RKE+LQHFKKPWAHEHEP +TL++AVKAIKP+ LIGTSG G+T
Sbjct: 363  ARKKIWLVDSKGLIVSSRKETLQHFKKPWAHEHEPLSTLLDAVKAIKPSVLIGTSGVGQT 422

Query: 1823 FTKEVIEAM 1849
            FT+ V+EAM
Sbjct: 423  FTQNVVEAM 431


>prf||1803524A malic enzyme
          Length = 589

 Score =  675 bits (1742), Expect = 0.0
 Identities = 339/431 (78%), Positives = 369/431 (85%), Gaps = 7/431 (1%)
 Frame = +2

Query: 578  SILKER----SLIEM---ATVAGGVQDVYGEDCATEDQLVTPWTISVASGYTLLRNPHHN 736
            S LKE     S+++M   +TV GGV+DVYGED ATEDQLVTPWTISVASGYTLLR+PHHN
Sbjct: 3    STLKEMRDGASVLDMDPKSTVGGGVEDVYGEDRATEDQLVTPWTISVASGYTLLRDPHHN 62

Query: 737  KGLAFTEKERDAHYLRGLLPPAVMSQQLQEKKLMQSLRSYDVPLHRYTALMELQERNERL 916
            KGLAFTEKERDAHYLRGLLPP  +SQQLQEKKLM ++R Y +PL +YTA+MEL+ERNERL
Sbjct: 63   KGLAFTEKERDAHYLRGLLPPTTISQQLQEKKLMNTIRQYQLPLQKYTAMMELEERNERL 122

Query: 917  FYKLLIDNVEELLPVVYTPTVGEACQKYGSIFKRPQGLYISXXXXXXXXXXXXNWPEKDI 1096
            FYKLLIDNVEELLPVVYTPTVGEACQKYGSIFKRPQGLYIS            NWP+K I
Sbjct: 123  FYKLLIDNVEELLPVVYTPTVGEACQKYGSIFKRPQGLYISLKEKGKVLDVLKNWPQKSI 182

Query: 1097 QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNQSLLDN 1276
            QVIVVTDGERILGLGDLGCQG+GIPVGKLSLYTALGGVRPSACLP+TIDVGTNN+ LL +
Sbjct: 183  QVIVVTDGERILGLGDLGCQGIGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEQLLKD 242

Query: 1277 EFYIGLKQRRATGKEYADLLHEFMAAVKQNYGEKVLVQFEDFANHNAFELLSKYSTTHLV 1456
            EFYIGL+QRRATG+EY++LLHEFM AVKQNYGEKVL+QFEDFANHNAF+LL+KY TTHLV
Sbjct: 243  EFYIGLRQRRATGQEYSELLHEFMTAVKQNYGEKVLIQFEDFANHNAFDLLAKYGTTHLV 302

Query: 1457 FNDDIQGTAXXXXXXXXXXXXXXXXTLADHTFLFLGAGEAGTGIAELIALEMSKQTNISL 1636
            FNDDIQGTA                +LADHTFLFLGAGEAGTGIAELIALEMS+++   L
Sbjct: 303  FNDDIQGTAAVVLAGLISALKLIGGSLADHTFLFLGAGEAGTGIAELIALEMSRRSKTPL 362

Query: 1637 EETRKKIWLVDSKGLIVSHRKESLQHFKKPWAHEHEPCTTLIEAVKAIKPTALIGTSGQG 1816
            EETRKKIWL DSKGLIVS RKESLQHFKKPWAHEHEP   L+E VKAIKP  LIGTSG G
Sbjct: 363  EETRKKIWLTDSKGLIVSSRKESLQHFKKPWAHEHEPVKGLLEVVKAIKPLVLIGTSGVG 422

Query: 1817 KTFTKEVIEAM 1849
            KTFTKEVIEAM
Sbjct: 423  KTFTKEVIEAM 433


>ref|XP_002324450.1| predicted protein [Populus trichocarpa] gi|222865884|gb|EEF03015.1|
            predicted protein [Populus trichocarpa]
          Length = 591

 Score =  675 bits (1741), Expect = 0.0
 Identities = 339/431 (78%), Positives = 370/431 (85%), Gaps = 7/431 (1%)
 Frame = +2

Query: 578  SILKER----SLIEM---ATVAGGVQDVYGEDCATEDQLVTPWTISVASGYTLLRNPHHN 736
            S LKE     S+++M   +TV GGV+DVYGED ATEDQLVTPWTISVASG+TLLR+P HN
Sbjct: 3    STLKEMRDGASVLDMDPKSTVGGGVEDVYGEDRATEDQLVTPWTISVASGFTLLRDPQHN 62

Query: 737  KGLAFTEKERDAHYLRGLLPPAVMSQQLQEKKLMQSLRSYDVPLHRYTALMELQERNERL 916
            KGLAFTEKERDAHYLRGLLPPA +SQQLQEKKLM ++R Y +PL +YTA+MEL+ERNERL
Sbjct: 63   KGLAFTEKERDAHYLRGLLPPATISQQLQEKKLMNTIRQYQLPLQKYTAMMELEERNERL 122

Query: 917  FYKLLIDNVEELLPVVYTPTVGEACQKYGSIFKRPQGLYISXXXXXXXXXXXXNWPEKDI 1096
            FYKLLIDNVEELLPVVYTPTVGEACQKYGSIFKRPQGLYIS            NWP+K I
Sbjct: 123  FYKLLIDNVEELLPVVYTPTVGEACQKYGSIFKRPQGLYISLKEKGKVLDVLKNWPQKSI 182

Query: 1097 QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNQSLLDN 1276
            QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLP+TIDVGTNN+ LL +
Sbjct: 183  QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEQLLKD 242

Query: 1277 EFYIGLKQRRATGKEYADLLHEFMAAVKQNYGEKVLVQFEDFANHNAFELLSKYSTTHLV 1456
            EFYIGL+Q+RATG+EY++LLHEFM AVKQNYGEKVL+QFEDFANHNAF+LL+KY TTHLV
Sbjct: 243  EFYIGLRQKRATGQEYSELLHEFMTAVKQNYGEKVLIQFEDFANHNAFDLLAKYGTTHLV 302

Query: 1457 FNDDIQGTAXXXXXXXXXXXXXXXXTLADHTFLFLGAGEAGTGIAELIALEMSKQTNISL 1636
            FNDDIQGTA                +LADHTFLFLGAGEAGTGIAELIALEMS+++   L
Sbjct: 303  FNDDIQGTAAVVLAGLISALKLLGGSLADHTFLFLGAGEAGTGIAELIALEMSRRSKTPL 362

Query: 1637 EETRKKIWLVDSKGLIVSHRKESLQHFKKPWAHEHEPCTTLIEAVKAIKPTALIGTSGQG 1816
            EETRKKIWL DSKGLIVS RKESLQHFKKPWAHEHEP   L+E VKAIKPT LIGTSG G
Sbjct: 363  EETRKKIWLTDSKGLIVSSRKESLQHFKKPWAHEHEPVKGLLEVVKAIKPTVLIGTSGVG 422

Query: 1817 KTFTKEVIEAM 1849
            KTFTKEVIEAM
Sbjct: 423  KTFTKEVIEAM 433