BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00000446
         (1549 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAS83468.1| beta-armyrin synthase [Bupleurum kaoi]                1032   0.0  
gb|ADK12003.1| beta-amyrin synthase [Aralia elata]                    931   0.0  
emb|CBI18407.3| unnamed protein product [Vitis vinifera]              913   0.0  
ref|XP_002270934.1| PREDICTED: beta-amyrin synthase [Vitis vinif...   913   0.0  
sp|O82140.1|BAMS1_PANGI RecName: Full=Beta-Amyrin Synthase 1 gi|...   912   0.0  

>gb|AAS83468.1| beta-armyrin synthase [Bupleurum kaoi]
          Length = 762

 Score = 1032 bits (2668), Expect = 0.0
 Identities = 488/498 (97%), Positives = 495/498 (99%)
 Frame = +3

Query: 3    YMPMSYLYGKRFVAPMTPLILQLREELYDQPYDHINWPKVRHCCAPEDIYYPHPLVQDLI 182
            YMPMSYLYGKRFVAPMTPLILQLREELYDQPYDHINWPKVRHCCAPEDIYYPHPLVQDLI
Sbjct: 265  YMPMSYLYGKRFVAPMTPLILQLREELYDQPYDHINWPKVRHCCAPEDIYYPHPLVQDLI 324

Query: 183  WDSLYICTEPLLTRWPFNKIREKALATTMKHIHYEDENSRYITIGCVEKVLCMLACWAED 362
            W SLYICTEPLLTRWPFNKIREKALATTM+HIHYEDENSRYITIGCVEKVLCM ACWAED
Sbjct: 325  WGSLYICTEPLLTRWPFNKIREKALATTMEHIHYEDENSRYITIGCVEKVLCMPACWAED 384

Query: 363  PDGDYFKKHLARIPDYLWVAEDGMKMQSFGSQEWDTGFGLQALLATDLTDELGPTLKRGH 542
            PDGDYFKKHLARIPDYLWVAEDGMKMQSFGSQEWDTGFGLQALLATDLTDELGPTLKRG 
Sbjct: 385  PDGDYFKKHLARIPDYLWVAEDGMKMQSFGSQEWDTGFGLQALLATDLTDELGPTLKRGR 444

Query: 543  EFVKASQVKDNPSGDFKSMYRHISKGSWTFSDQDHGWQVSDCTAEGLKCCLLFSMMSPEI 722
            EFVKASQVKDNPSGDFKSMYRHISKGSWTFSDQDHGWQVSDCTAEGLKCCLLFSMMSPEI
Sbjct: 445  EFVKASQVKDNPSGDFKSMYRHISKGSWTFSDQDHGWQVSDCTAEGLKCCLLFSMMSPEI 504

Query: 723  VGKKFEPERLYDSVNVLLSLQSKNGGLAAWEPAGAQEWLELLNPTEFFQDIVIEHEYVEC 902
            VGKKFEPERLYDSVN+LLSLQSKNGGLAAWEPAGAQEWLELLNPTEFFQDIVIEHEYVEC
Sbjct: 505  VGKKFEPERLYDSVNLLLSLQSKNGGLAAWEPAGAQEWLELLNPTEFFQDIVIEHEYVEC 564

Query: 903  TASAIQALVLFSKLYPAHRKKEINAFIVNAAKYLEDIQMPDGSWYGNWGVCFTYGSWFAL 1082
            TASAIQALVLF+KLYPAHRKKEINAFIVNAAKYLEDIQMPDGSWYGNWGVCFTYGSWFAL
Sbjct: 565  TASAIQALVLFTKLYPAHRKKEINAFIVNAAKYLEDIQMPDGSWYGNWGVCFTYGSWFAL 624

Query: 1083 GGLAAAGKTYNNCAAVRKGVDFLLKSQLDDGGWGESYLSCPLKKYTQIEGSRSNLVQTAW 1262
            GGLAAAGKTYNNCAAVRKGVDFLLKSQLDDGGWGESYLSCPL+KYTQIEGSRSNLVQTAW
Sbjct: 625  GGLAAAGKTYNNCAAVRKGVDFLLKSQLDDGGWGESYLSCPLEKYTQIEGSRSNLVQTAW 684

Query: 1263 ATMGLIHSGQVERDPTPLHRAAKLIINSQEEKGGFPQQEITGVFMKNCMLHYAAYRNLYP 1442
            AT+GLIHSGQVERDPTPLHRAAKLIINSQEEKGGFPQQEITGVFMKNCMLHYAAYR+LYP
Sbjct: 685  ATVGLIHSGQVERDPTPLHRAAKLIINSQEEKGGFPQQEITGVFMKNCMLHYAAYRDLYP 744

Query: 1443 LWALAEYRKRVALPSSSI 1496
            LWALAEYRKRVALPSS+I
Sbjct: 745  LWALAEYRKRVALPSSTI 762


>gb|ADK12003.1| beta-amyrin synthase [Aralia elata]
          Length = 763

 Score =  931 bits (2406), Expect = 0.0
 Identities = 432/495 (87%), Positives = 457/495 (92%)
 Frame = +3

Query: 3    YMPMSYLYGKRFVAPMTPLILQLREELYDQPYDHINWPKVRHCCAPEDIYYPHPLVQDLI 182
            YMPMSYLYGKRFV P+TPLILQLREELY QPY+ INW K RH CA EDIYYPHPL+QDL+
Sbjct: 266  YMPMSYLYGKRFVGPITPLILQLREELYAQPYNEINWRKTRHVCAKEDIYYPHPLIQDLL 325

Query: 183  WDSLYICTEPLLTRWPFNKIREKALATTMKHIHYEDENSRYITIGCVEKVLCMLACWAED 362
            WDSLY+ TEPLLTRWPFNK+REKAL TTMKHIHYEDENSRYITIGCVEKVLCMLACW ED
Sbjct: 326  WDSLYVLTEPLLTRWPFNKLREKALQTTMKHIHYEDENSRYITIGCVEKVLCMLACWVED 385

Query: 363  PDGDYFKKHLARIPDYLWVAEDGMKMQSFGSQEWDTGFGLQALLATDLTDELGPTLKRGH 542
            P+GDYFKKHLARIPDY+WVAEDGMKMQSFGSQEWDTGFG+QALLA+DLT E+GPTL +GH
Sbjct: 386  PNGDYFKKHLARIPDYIWVAEDGMKMQSFGSQEWDTGFGIQALLASDLTHEIGPTLMKGH 445

Query: 543  EFVKASQVKDNPSGDFKSMYRHISKGSWTFSDQDHGWQVSDCTAEGLKCCLLFSMMSPEI 722
            +F+K SQVKDNPSGDFK MYRHISKGSWTFSDQDHGWQVSDCTAEGLKCCL+FS M  EI
Sbjct: 446  DFIKKSQVKDNPSGDFKGMYRHISKGSWTFSDQDHGWQVSDCTAEGLKCCLIFSTMPEEI 505

Query: 723  VGKKFEPERLYDSVNVLLSLQSKNGGLAAWEPAGAQEWLELLNPTEFFQDIVIEHEYVEC 902
            VGKK EPERLYDSVNVLLSLQSKNGGLAAWEPAGAQEWLELLNPTEFF DIVIEHEYVEC
Sbjct: 506  VGKKMEPERLYDSVNVLLSLQSKNGGLAAWEPAGAQEWLELLNPTEFFADIVIEHEYVEC 565

Query: 903  TASAIQALVLFSKLYPAHRKKEINAFIVNAAKYLEDIQMPDGSWYGNWGVCFTYGSWFAL 1082
            T+SAIQALVLF KLYP HRKKEI+ FI+NA +YLEDIQMPDGSWYGNWGVCFTYGSWFAL
Sbjct: 566  TSSAIQALVLFKKLYPGHRKKEIDNFIMNAVRYLEDIQMPDGSWYGNWGVCFTYGSWFAL 625

Query: 1083 GGLAAAGKTYNNCAAVRKGVDFLLKSQLDDGGWGESYLSCPLKKYTQIEGSRSNLVQTAW 1262
            GGLAAAGKTY NCAAVRK VDFLLKSQ+DDGGWGESYLSCP K Y  +EG+RSNLV T W
Sbjct: 626  GGLAAAGKTYYNCAAVRKAVDFLLKSQMDDGGWGESYLSCPKKVYVPLEGNRSNLVHTGW 685

Query: 1263 ATMGLIHSGQVERDPTPLHRAAKLIINSQEEKGGFPQQEITGVFMKNCMLHYAAYRNLYP 1442
            A MGLIHS Q ERDPTPLHRAAKL+INSQ E G FPQQEITGVFMKNCMLHYAAYRN+YP
Sbjct: 686  ALMGLIHSEQAERDPTPLHRAAKLLINSQMEDGDFPQQEITGVFMKNCMLHYAAYRNIYP 745

Query: 1443 LWALAEYRKRVALPS 1487
            LWALAEYR+RV LPS
Sbjct: 746  LWALAEYRRRVPLPS 760


>emb|CBI18407.3| unnamed protein product [Vitis vinifera]
          Length = 846

 Score =  913 bits (2359), Expect = 0.0
 Identities = 420/494 (85%), Positives = 454/494 (91%)
 Frame = +3

Query: 3    YMPMSYLYGKRFVAPMTPLILQLREELYDQPYDHINWPKVRHCCAPEDIYYPHPLVQDLI 182
            YMPMSYLYGKRFV P+TPL+L+LREEL+ QPY+ INW KVRH CA ED+YYPHPL+QDL+
Sbjct: 353  YMPMSYLYGKRFVGPITPLVLELREELFLQPYNEINWRKVRHLCAKEDLYYPHPLIQDLM 412

Query: 183  WDSLYICTEPLLTRWPFNKIREKALATTMKHIHYEDENSRYITIGCVEKVLCMLACWAED 362
            WD LYICTEPLLTRWPFNK+R+KAL  TMKHIHYEDENSRYITIGCVEKVLCML+CW ED
Sbjct: 413  WDGLYICTEPLLTRWPFNKLRQKALEVTMKHIHYEDENSRYITIGCVEKVLCMLSCWVED 472

Query: 363  PDGDYFKKHLARIPDYLWVAEDGMKMQSFGSQEWDTGFGLQALLATDLTDELGPTLKRGH 542
            P+G+YFKKHLARIPDY+WV EDG+KMQSFGSQEWDTGF LQA+LA ++TDE+GPTLK+GH
Sbjct: 473  PNGNYFKKHLARIPDYIWVGEDGIKMQSFGSQEWDTGFALQAVLACNMTDEIGPTLKKGH 532

Query: 543  EFVKASQVKDNPSGDFKSMYRHISKGSWTFSDQDHGWQVSDCTAEGLKCCLLFSMMSPEI 722
            EFVK SQVKDNPSGDFKSMYRHISKGSWTFSDQDHGWQVSDCTAEGLKCCLLFSMM PEI
Sbjct: 533  EFVKESQVKDNPSGDFKSMYRHISKGSWTFSDQDHGWQVSDCTAEGLKCCLLFSMMPPEI 592

Query: 723  VGKKFEPERLYDSVNVLLSLQSKNGGLAAWEPAGAQEWLELLNPTEFFQDIVIEHEYVEC 902
            VG K EPERL+DSVN+LLSLQSKNGGLAAWEPAGA EWLELLNPTEFF DIVIEHEYVEC
Sbjct: 593  VGVKMEPERLFDSVNILLSLQSKNGGLAAWEPAGASEWLELLNPTEFFADIVIEHEYVEC 652

Query: 903  TASAIQALVLFSKLYPAHRKKEINAFIVNAAKYLEDIQMPDGSWYGNWGVCFTYGSWFAL 1082
            TASAIQALVLF KLYP HRKKEI+ FI  AAKY+EDIQMPDGSWYGNWGVCFTYGSWFAL
Sbjct: 653  TASAIQALVLFKKLYPGHRKKEIDNFITYAAKYIEDIQMPDGSWYGNWGVCFTYGSWFAL 712

Query: 1083 GGLAAAGKTYNNCAAVRKGVDFLLKSQLDDGGWGESYLSCPLKKYTQIEGSRSNLVQTAW 1262
            GGLAAAGKTY+NC A+R+ V+FLL SQ DDGGWGESY+SCP KKYT +EG+RSNLV T W
Sbjct: 713  GGLAAAGKTYHNCHAIRRAVEFLLNSQRDDGGWGESYVSCPDKKYTPLEGNRSNLVHTGW 772

Query: 1263 ATMGLIHSGQVERDPTPLHRAAKLIINSQEEKGGFPQQEITGVFMKNCMLHYAAYRNLYP 1442
            A MGLI SGQ ERDPTPLHRAAKL+IN Q E G FPQQEITGVFMKNCMLHYAAYRN+YP
Sbjct: 773  ALMGLISSGQAERDPTPLHRAAKLLINFQMEDGDFPQQEITGVFMKNCMLHYAAYRNIYP 832

Query: 1443 LWALAEYRKRVALP 1484
            LWALAEYR+RV LP
Sbjct: 833  LWALAEYRRRVPLP 846


>ref|XP_002270934.1| PREDICTED: beta-amyrin synthase [Vitis vinifera]
          Length = 757

 Score =  913 bits (2359), Expect = 0.0
 Identities = 420/494 (85%), Positives = 454/494 (91%)
 Frame = +3

Query: 3    YMPMSYLYGKRFVAPMTPLILQLREELYDQPYDHINWPKVRHCCAPEDIYYPHPLVQDLI 182
            YMPMSYLYGKRFV P+TPL+L+LREEL+ QPY+ INW KVRH CA ED+YYPHPL+QDL+
Sbjct: 264  YMPMSYLYGKRFVGPITPLVLELREELFLQPYNEINWRKVRHLCAKEDLYYPHPLIQDLM 323

Query: 183  WDSLYICTEPLLTRWPFNKIREKALATTMKHIHYEDENSRYITIGCVEKVLCMLACWAED 362
            WD LYICTEPLLTRWPFNK+R+KAL  TMKHIHYEDENSRYITIGCVEKVLCML+CW ED
Sbjct: 324  WDGLYICTEPLLTRWPFNKLRQKALEVTMKHIHYEDENSRYITIGCVEKVLCMLSCWVED 383

Query: 363  PDGDYFKKHLARIPDYLWVAEDGMKMQSFGSQEWDTGFGLQALLATDLTDELGPTLKRGH 542
            P+G+YFKKHLARIPDY+WV EDG+KMQSFGSQEWDTGF LQA+LA ++TDE+GPTLK+GH
Sbjct: 384  PNGNYFKKHLARIPDYIWVGEDGIKMQSFGSQEWDTGFALQAVLACNMTDEIGPTLKKGH 443

Query: 543  EFVKASQVKDNPSGDFKSMYRHISKGSWTFSDQDHGWQVSDCTAEGLKCCLLFSMMSPEI 722
            EFVK SQVKDNPSGDFKSMYRHISKGSWTFSDQDHGWQVSDCTAEGLKCCLLFSMM PEI
Sbjct: 444  EFVKESQVKDNPSGDFKSMYRHISKGSWTFSDQDHGWQVSDCTAEGLKCCLLFSMMPPEI 503

Query: 723  VGKKFEPERLYDSVNVLLSLQSKNGGLAAWEPAGAQEWLELLNPTEFFQDIVIEHEYVEC 902
            VG K EPERL+DSVN+LLSLQSKNGGLAAWEPAGA EWLELLNPTEFF DIVIEHEYVEC
Sbjct: 504  VGVKMEPERLFDSVNILLSLQSKNGGLAAWEPAGASEWLELLNPTEFFADIVIEHEYVEC 563

Query: 903  TASAIQALVLFSKLYPAHRKKEINAFIVNAAKYLEDIQMPDGSWYGNWGVCFTYGSWFAL 1082
            TASAIQALVLF KLYP HRKKEI+ FI  AAKY+EDIQMPDGSWYGNWGVCFTYGSWFAL
Sbjct: 564  TASAIQALVLFKKLYPGHRKKEIDNFITYAAKYIEDIQMPDGSWYGNWGVCFTYGSWFAL 623

Query: 1083 GGLAAAGKTYNNCAAVRKGVDFLLKSQLDDGGWGESYLSCPLKKYTQIEGSRSNLVQTAW 1262
            GGLAAAGKTY+NC A+R+ V+FLL SQ DDGGWGESY+SCP KKYT +EG+RSNLV T W
Sbjct: 624  GGLAAAGKTYHNCHAIRRAVEFLLNSQRDDGGWGESYVSCPDKKYTPLEGNRSNLVHTGW 683

Query: 1263 ATMGLIHSGQVERDPTPLHRAAKLIINSQEEKGGFPQQEITGVFMKNCMLHYAAYRNLYP 1442
            A MGLI SGQ ERDPTPLHRAAKL+IN Q E G FPQQEITGVFMKNCMLHYAAYRN+YP
Sbjct: 684  ALMGLISSGQAERDPTPLHRAAKLLINFQMEDGDFPQQEITGVFMKNCMLHYAAYRNIYP 743

Query: 1443 LWALAEYRKRVALP 1484
            LWALAEYR+RV LP
Sbjct: 744  LWALAEYRRRVPLP 757


>sp|O82140.1|BAMS1_PANGI RecName: Full=Beta-Amyrin Synthase 1 gi|3688600|dbj|BAA33461.1|
            beta-Amyrin Synthase [Panax ginseng]
          Length = 763

 Score =  912 bits (2358), Expect = 0.0
 Identities = 422/495 (85%), Positives = 451/495 (91%)
 Frame = +3

Query: 3    YMPMSYLYGKRFVAPMTPLILQLREELYDQPYDHINWPKVRHCCAPEDIYYPHPLVQDLI 182
            YMPMSYLYGKRFV P+TPLILQLREELY QPY+ INW K R  CA EDIYYPHPL+QDL+
Sbjct: 266  YMPMSYLYGKRFVGPITPLILQLREELYGQPYNEINWRKTRRVCAKEDIYYPHPLIQDLL 325

Query: 183  WDSLYICTEPLLTRWPFNKIREKALATTMKHIHYEDENSRYITIGCVEKVLCMLACWAED 362
            WDSLY+ TEPLLTRWPFNK+REKAL TTMKHIHYEDENSRYITIGCVEKVLCML CW ED
Sbjct: 326  WDSLYVLTEPLLTRWPFNKLREKALQTTMKHIHYEDENSRYITIGCVEKVLCMLVCWVED 385

Query: 363  PDGDYFKKHLARIPDYLWVAEDGMKMQSFGSQEWDTGFGLQALLATDLTDELGPTLKRGH 542
            P+GDYF+KHLARIPDY+WVAEDGMKMQSFGSQEWDTGF +QALL +DLT E+GPTL +GH
Sbjct: 386  PNGDYFRKHLARIPDYIWVAEDGMKMQSFGSQEWDTGFSIQALLDSDLTHEIGPTLMKGH 445

Query: 543  EFVKASQVKDNPSGDFKSMYRHISKGSWTFSDQDHGWQVSDCTAEGLKCCLLFSMMSPEI 722
            +F+K SQVKDNPSGDFKSMYRHISKGSWTFSDQDHGWQVSDCTAEGLKCCL+FS M  EI
Sbjct: 446  DFIKKSQVKDNPSGDFKSMYRHISKGSWTFSDQDHGWQVSDCTAEGLKCCLIFSTMPEEI 505

Query: 723  VGKKFEPERLYDSVNVLLSLQSKNGGLAAWEPAGAQEWLELLNPTEFFQDIVIEHEYVEC 902
            VGKK +PERLYDSVNVLLSLQ KNGGL+AWEPAGAQEWLELLNPTEFF DIVIEHEYVEC
Sbjct: 506  VGKKIKPERLYDSVNVLLSLQRKNGGLSAWEPAGAQEWLELLNPTEFFADIVIEHEYVEC 565

Query: 903  TASAIQALVLFSKLYPAHRKKEINAFIVNAAKYLEDIQMPDGSWYGNWGVCFTYGSWFAL 1082
            T+SAIQALVLF KLYP HRKKEI+ FI NA +YLED QMPDGSWYGNWGVCFTYGSWFAL
Sbjct: 566  TSSAIQALVLFKKLYPGHRKKEIDNFITNAVRYLEDTQMPDGSWYGNWGVCFTYGSWFAL 625

Query: 1083 GGLAAAGKTYNNCAAVRKGVDFLLKSQLDDGGWGESYLSCPLKKYTQIEGSRSNLVQTAW 1262
            GGLAAAGKTY NCAAVRK V+FLLKSQ+DDGGWGESYLSCP K Y  +EG+RSNLV T W
Sbjct: 626  GGLAAAGKTYYNCAAVRKAVEFLLKSQMDDGGWGESYLSCPKKVYVPLEGNRSNLVHTGW 685

Query: 1263 ATMGLIHSGQVERDPTPLHRAAKLIINSQEEKGGFPQQEITGVFMKNCMLHYAAYRNLYP 1442
            A MGLIHS Q ERDPTPLHRAAKL+INSQ E G FPQQEI+GVFMKNCMLHYAAYRN+YP
Sbjct: 686  ALMGLIHSEQAERDPTPLHRAAKLLINSQMEDGDFPQQEISGVFMKNCMLHYAAYRNIYP 745

Query: 1443 LWALAEYRKRVALPS 1487
            LWALAEYR+RV LPS
Sbjct: 746  LWALAEYRRRVPLPS 760