BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00000423
         (1633 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002331405.1| predicted protein [Populus trichocarpa] gi|2...   350   8e-94
ref|XP_003532450.1| PREDICTED: tRNA pseudouridine synthase A-lik...   343   6e-92
ref|XP_002275354.2| PREDICTED: tRNA pseudouridine synthase A-lik...   343   1e-91
emb|CBI33811.3| unnamed protein product [Vitis vinifera]              336   1e-89
ref|XP_003524428.1| PREDICTED: tRNA pseudouridine synthase A-lik...   334   4e-89

>ref|XP_002331405.1| predicted protein [Populus trichocarpa] gi|222873619|gb|EEF10750.1|
            predicted protein [Populus trichocarpa]
          Length = 428

 Score =  350 bits (897), Expect = 8e-94
 Identities = 193/353 (54%), Positives = 232/353 (65%), Gaps = 12/353 (3%)
 Frame = -3

Query: 1196 KSGRWARVTFKIVLSYSGGSFDGWQRQPGLNTVQGCVERSXXXXXXXXXXXXXXXXXLPI 1017
            KSGRWARVTFKIVLSY GGSFDGWQ+QPGLNTVQG VE+S                  P+
Sbjct: 98   KSGRWARVTFKIVLSYHGGSFDGWQKQPGLNTVQGLVEKSLGRFVDEKKAQQLKEQCKPL 157

Query: 1016 EGCALVAGRTDKGVTAYQQVCSFYTWRNDVSTKDIGEAINNTAPGKLRVISVSKVSRAFH 837
            EGCA VAGRTDKGV+A QQVCSFYTWR DV   +I +AIN+ APGK+RV S+S+VSRAFH
Sbjct: 158  EGCASVAGRTDKGVSALQQVCSFYTWRKDVKPHEIEDAINDVAPGKVRVESISEVSRAFH 217

Query: 836  PNFSAKWRHYMYIFPL-DGEDEASIQCEESDGGLKSGFDHVH----------MAAEDS-D 693
            PNFSAKWR Y+YIFPL DGE+   I   E +GG+++   H +          +A+E++ +
Sbjct: 218  PNFSAKWRRYLYIFPLNDGENREEI---EGEGGIENFSSHENCEKQRNECGELASEENVE 274

Query: 692  LQADSDNFEIRSCQKSNRFSVKKVDLLLRQLEGKLLSYKMFARDTKASRNIGPPTECFVF 513
                SD  E++  +K   FSV +V+ LL+QLEGKLLSYKMFARDTKASRN+GPPTECF++
Sbjct: 275  NSIISDEDELQGAKKPRSFSVCRVNQLLQQLEGKLLSYKMFARDTKASRNVGPPTECFLY 334

Query: 512  HARATEIKLPSDAKDEEGAXXXXXXXXXXXXXXXXXXXXAMCIELVANRFLRKMVRVLVX 333
            HARATE +LPS   ++                        MC+EL+ANRFLRKMVRVLV 
Sbjct: 335  HARATETRLPSPDHEK--------------------GIRVMCVELIANRFLRKMVRVLVA 374

Query: 332  XXXXXXXXXXXXXALVKLMDXXXXXXXXXXXXPDGLCLVDVGYTEFNPKVCLI 174
                         AL+KLMD            PDGLCLVDVGYTEF+P+ CLI
Sbjct: 375  TSVREAAAGAQEDALLKLMDATCRRATAPPAPPDGLCLVDVGYTEFDPRNCLI 427



 Score = 79.3 bits (194), Expect = 3e-12
 Identities = 32/49 (65%), Positives = 41/49 (83%)
 Frame = -1

Query: 1528 YIQYNHTDACKFSRWTSRESYQFMYARSWQKVNDFYSNLVRGNISFSDI 1382
            Y+QYNHTD+CKFSRWT+RES+QFM+AR WQ+V DFYS  V G +S  ++
Sbjct: 9    YVQYNHTDSCKFSRWTARESFQFMHARPWQEVVDFYSKSVNGQLSLLEL 57


>ref|XP_003532450.1| PREDICTED: tRNA pseudouridine synthase A-like [Glycine max]
          Length = 404

 Score =  343 bits (881), Expect = 6e-92
 Identities = 189/351 (53%), Positives = 220/351 (62%)
 Frame = -3

Query: 1223 ELVSSPTCKKSGRWARVTFKIVLSYSGGSFDGWQRQPGLNTVQGCVERSXXXXXXXXXXX 1044
            EL S  +  +SGRWAR TFKI+LSY GGSFDGWQ+QPGLNTVQ  VE S           
Sbjct: 81   ELESVASEDRSGRWARTTFKIILSYHGGSFDGWQKQPGLNTVQSIVEGSLGKFVDEKKTQ 140

Query: 1043 XXXXXXLPIEGCALVAGRTDKGVTAYQQVCSFYTWRNDVSTKDIGEAINNTAPGKLRVIS 864
                  LPIEGCA+VAGRTDKGVT  QQVCSFYTW+ DV  +DI +AIN+ APGKLRVIS
Sbjct: 141  LLKDKGLPIEGCAIVAGRTDKGVTGLQQVCSFYTWKKDVKPRDIEDAINHAAPGKLRVIS 200

Query: 863  VSKVSRAFHPNFSAKWRHYMYIFPLDGEDEASIQCEESDGGLKSGFDHVHMAAEDSDLQA 684
            VS+VSR FHPNFSAKWR Y+YIFPL          +  +      ++ +H +A   +L+ 
Sbjct: 201  VSEVSRVFHPNFSAKWRRYLYIFPLTNGGYTDQSSDNGEFYDSFKYNEIHDSASKDELEN 260

Query: 683  DSDNFEIRSCQKSNRFSVKKVDLLLRQLEGKLLSYKMFARDTKASRNIGPPTECFVFHAR 504
            +         +KS  FS+ KV+ LLR+LEGKLLSYKMFARDTKASRN GPPTECFV+HAR
Sbjct: 261  E---------KKSYAFSISKVNRLLRKLEGKLLSYKMFARDTKASRNDGPPTECFVYHAR 311

Query: 503  ATEIKLPSDAKDEEGAXXXXXXXXXXXXXXXXXXXXAMCIELVANRFLRKMVRVLVXXXX 324
            A E+KLP+    EE                       MC+ELVANRFLRKMVRVLV    
Sbjct: 312  AMEVKLPATDHGEE--------------------TKVMCVELVANRFLRKMVRVLVATSI 351

Query: 323  XXXXXXXXXXALVKLMDXXXXXXXXXXXXPDGLCLVDVGYTEFNPKVCLIV 171
                      AL+KLMD            PDGLCLVDVGYTEF P+ C I+
Sbjct: 352  REAAAGAEDDALIKLMDATCRRATAPPAPPDGLCLVDVGYTEFEPQKCFIL 402



 Score = 73.2 bits (178), Expect = 2e-10
 Identities = 28/45 (62%), Positives = 38/45 (84%)
 Frame = -1

Query: 1528 YIQYNHTDACKFSRWTSRESYQFMYARSWQKVNDFYSNLVRGNIS 1394
            ++ YNHT   K++RWT+RES++FMYAR WQ+VNDFY N+VR N+S
Sbjct: 10   HVHYNHTHPSKYARWTARESFEFMYARPWQRVNDFYLNIVRANLS 54


>ref|XP_002275354.2| PREDICTED: tRNA pseudouridine synthase A-like [Vitis vinifera]
          Length = 433

 Score =  343 bits (879), Expect = 1e-91
 Identities = 195/355 (54%), Positives = 222/355 (62%), Gaps = 11/355 (3%)
 Frame = -3

Query: 1205 TCKKSGRWARVTFKIVLSYSGGSFDGWQRQPGLNTVQGCVERSXXXXXXXXXXXXXXXXX 1026
            T  + GRWAR+TFKIVLSY G SFDGWQ+QPGLNTVQG VE S                 
Sbjct: 98   TKDRGGRWARLTFKIVLSYHGASFDGWQKQPGLNTVQGLVEASLGRFVDERKAQQLKEKN 157

Query: 1025 LPIEGCALVAGRTDKGVTAYQQVCSFYTWRNDVSTKDIGEAINNTAPGKLRVISVSKVSR 846
            LP+EG   VAGRTDKGVTA QQVCSFYTW+ DV  +DI +AIN  APGKLRVISVS+VSR
Sbjct: 158  LPVEGMVTVAGRTDKGVTALQQVCSFYTWKKDVKVQDIEDAINGAAPGKLRVISVSEVSR 217

Query: 845  AFHPNFSAKWRHYMYIFPL-DGEDEASIQCEESD----------GGLKSGFDHVHMAAED 699
             FHPNFSAKWRHY YIFPL DG+D   I   E D          G   S     +   E+
Sbjct: 218  IFHPNFSAKWRHYFYIFPLNDGDDGEQISENEMDIQKISFNEKYGEQGSECPAQYNGGEN 277

Query: 698  SDLQADSDNFEIRSCQKSNRFSVKKVDLLLRQLEGKLLSYKMFARDTKASRNIGPPTECF 519
            ++     D+ E  S +K + FS+ +VD LLRQLEGKLLSYK+FARDTKASRNIGPPTECF
Sbjct: 278  TENLIFDDDGEFESRKKPSTFSISRVDQLLRQLEGKLLSYKIFARDTKASRNIGPPTECF 337

Query: 518  VFHARATEIKLPSDAKDEEGAXXXXXXXXXXXXXXXXXXXXAMCIELVANRFLRKMVRVL 339
            VFHARATE +L   + D EG                      MCIELVANRFLRKMVRVL
Sbjct: 338  VFHARATEARL-QFSDDGEGT-------------------KVMCIELVANRFLRKMVRVL 377

Query: 338  VXXXXXXXXXXXXXXALVKLMDXXXXXXXXXXXXPDGLCLVDVGYTEFNPKVCLI 174
            V              AL+KLMD            PDGLCL+DVG+TEF+P++C I
Sbjct: 378  VATSIREAAAGAEQDALLKLMDATCRRATAPPAPPDGLCLIDVGFTEFDPQICFI 432



 Score = 68.6 bits (166), Expect = 5e-09
 Identities = 29/45 (64%), Positives = 34/45 (75%)
 Frame = -1

Query: 1528 YIQYNHTDACKFSRWTSRESYQFMYARSWQKVNDFYSNLVRGNIS 1394
            Y+ Y HT   KFSRWT+RES+QFMY R WQ+V DFYSN+V G  S
Sbjct: 23   YVHYTHTHPTKFSRWTARESHQFMYKRPWQQVLDFYSNVVTGRCS 67


>emb|CBI33811.3| unnamed protein product [Vitis vinifera]
          Length = 402

 Score =  336 bits (861), Expect = 1e-89
 Identities = 191/345 (55%), Positives = 217/345 (62%), Gaps = 1/345 (0%)
 Frame = -3

Query: 1205 TCKKSGRWARVTFKIVLSYSGGSFDGWQRQPGLNTVQGCVERSXXXXXXXXXXXXXXXXX 1026
            T  + GRWAR+TFKIVLSY G SFDGWQ+QPGLNTVQG VE S                 
Sbjct: 98   TKDRGGRWARLTFKIVLSYHGASFDGWQKQPGLNTVQGLVEASLGRFVDERKAQQLKEKN 157

Query: 1025 LPIEGCALVAGRTDKGVTAYQQVCSFYTWRNDVSTKDIGEAINNTAPGKLRVISVSKVSR 846
            LP+EG   VAGRTDKGVTA QQVCSFYTW+ DV  +DI +AIN  APGKLRVISVS+VSR
Sbjct: 158  LPVEGMVTVAGRTDKGVTALQQVCSFYTWKKDVKVQDIEDAINGAAPGKLRVISVSEVSR 217

Query: 845  AFHPNFSAKWRHYMYIFPL-DGEDEASIQCEESDGGLKSGFDHVHMAAEDSDLQADSDNF 669
             FHPNFSAKWRHY YIFPL DG+D   I                  +  + D+Q  S N 
Sbjct: 218  IFHPNFSAKWRHYFYIFPLNDGDDGEQI------------------SENEMDIQKISFN- 258

Query: 668  EIRSCQKSNRFSVKKVDLLLRQLEGKLLSYKMFARDTKASRNIGPPTECFVFHARATEIK 489
               + +K + FS+ +VD LLRQLEGKLLSYK+FARDTKASRNIGPPTECFVFHARATE +
Sbjct: 259  --ENRKKPSTFSISRVDQLLRQLEGKLLSYKIFARDTKASRNIGPPTECFVFHARATEAR 316

Query: 488  LPSDAKDEEGAXXXXXXXXXXXXXXXXXXXXAMCIELVANRFLRKMVRVLVXXXXXXXXX 309
            L   + D EG                      MCIELVANRFLRKMVRVLV         
Sbjct: 317  L-QFSDDGEGT-------------------KVMCIELVANRFLRKMVRVLVATSIREAAA 356

Query: 308  XXXXXALVKLMDXXXXXXXXXXXXPDGLCLVDVGYTEFNPKVCLI 174
                 AL+KLMD            PDGLCL+DVG+TEF+P++C I
Sbjct: 357  GAEQDALLKLMDATCRRATAPPAPPDGLCLIDVGFTEFDPQICFI 401



 Score = 68.6 bits (166), Expect = 5e-09
 Identities = 29/45 (64%), Positives = 34/45 (75%)
 Frame = -1

Query: 1528 YIQYNHTDACKFSRWTSRESYQFMYARSWQKVNDFYSNLVRGNIS 1394
            Y+ Y HT   KFSRWT+RES+QFMY R WQ+V DFYSN+V G  S
Sbjct: 23   YVHYTHTHPTKFSRWTARESHQFMYKRPWQQVLDFYSNVVTGRCS 67


>ref|XP_003524428.1| PREDICTED: tRNA pseudouridine synthase A-like [Glycine max]
          Length = 387

 Score =  334 bits (857), Expect = 4e-89
 Identities = 187/351 (53%), Positives = 219/351 (62%)
 Frame = -3

Query: 1223 ELVSSPTCKKSGRWARVTFKIVLSYSGGSFDGWQRQPGLNTVQGCVERSXXXXXXXXXXX 1044
            EL S  T  +SGR AR TFKI+LSY GGSFDGWQ+QPGLNTVQ  VE S           
Sbjct: 64   ELESVATEDRSGRRARTTFKIILSYHGGSFDGWQKQPGLNTVQSIVEGSLGKFVDEKKTQ 123

Query: 1043 XXXXXXLPIEGCALVAGRTDKGVTAYQQVCSFYTWRNDVSTKDIGEAINNTAPGKLRVIS 864
                  LPIEGCA+VAGRTDKGVT  QQVCSFYTW+ DV  +DI +AIN+  PGK RVIS
Sbjct: 124  LLKDNGLPIEGCAVVAGRTDKGVTGLQQVCSFYTWKKDVKPRDIEDAINHATPGKFRVIS 183

Query: 863  VSKVSRAFHPNFSAKWRHYMYIFPLDGEDEASIQCEESDGGLKSGFDHVHMAAEDSDLQA 684
            VS+VSR FHPNFSAKWR Y+YIFPL          + +D G      H + + + +++  
Sbjct: 184  VSEVSRVFHPNFSAKWRRYLYIFPL---TNGRYTDQSNDNG------HFYNSCKYNEIHD 234

Query: 683  DSDNFEIRSCQKSNRFSVKKVDLLLRQLEGKLLSYKMFARDTKASRNIGPPTECFVFHAR 504
             +   E+ + +KS  FS  KV+ LLR+LEGKLLSYKMFARDTKASRN GPPTECFV+HAR
Sbjct: 235  SASKDELENGKKSYMFSTSKVNRLLRKLEGKLLSYKMFARDTKASRNDGPPTECFVYHAR 294

Query: 503  ATEIKLPSDAKDEEGAXXXXXXXXXXXXXXXXXXXXAMCIELVANRFLRKMVRVLVXXXX 324
            A E+K P+    EE                       MC+ELVA+RFLRKMVRVLV    
Sbjct: 295  AMEVKPPTTDHGEE--------------------TKVMCVELVADRFLRKMVRVLVATSI 334

Query: 323  XXXXXXXXXXALVKLMDXXXXXXXXXXXXPDGLCLVDVGYTEFNPKVCLIV 171
                      AL+KLMD            PDGLCLVDVGYTEF+PK C I+
Sbjct: 335  REAAAGAEDDALIKLMDATCRRATAPPAPPDGLCLVDVGYTEFDPKKCFIL 385