BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00000408
         (1775 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ACM48123.1| calcium antiporter 1 [Malus x domestica] gi|22358...   293   e-138
ref|XP_004148618.1| PREDICTED: vacuolar cation/proton exchanger ...   286   e-136
ref|XP_002274791.2| PREDICTED: vacuolar cation/proton exchanger ...   280   e-135
gb|AET87472.1| Ca2+/H+ exchanging protein [Morus alba var. multi...   280   e-135
ref|XP_002528100.1| Vacuolar cation/proton exchanger 1a, putativ...   275   e-135

>gb|ACM48123.1| calcium antiporter 1 [Malus x domestica] gi|223587975|gb|ACM48122.1|
            calcium antiporter 1 [Malus x domestica]
          Length = 450

 Score =  293 bits (750), Expect(2) = e-138
 Identities = 144/193 (74%), Positives = 166/193 (86%)
 Frame = +1

Query: 820  TLQLSRVSSIIMLVAYIAYLVFQLWTHREFFEAQXXXXXXLVSEEAPALGFWSAFIWLVG 999
            TL LSR SSI+ML+AY AY+VFQLWTHRE FEAQ       V EE P +GFWS  +WLVG
Sbjct: 228  TLHLSRASSIVMLLAYAAYIVFQLWTHRELFEAQEESDDDDVVEETPDIGFWSGIVWLVG 287

Query: 1000 MTVVIAFLSEYVVGTIEQAAATWGISVSFISIILLPIVGNAAEHAGAVIFAFKNKLDITL 1179
            MT VIA LSEYVVGTIE+AA +WG+SVSFISIILLPIVGNAAEHAGA+IFAFKNKLDITL
Sbjct: 288  MTAVIALLSEYVVGTIEEAADSWGLSVSFISIILLPIVGNAAEHAGAIIFAFKNKLDITL 347

Query: 1180 GVALGSATQIALFVVPVSVTFAWILDINMNLDFNLLETGSLVLSVLVVAFTLQDGTSHYM 1359
            GVALGSATQIA+FVVP+ V  AWI+ INM+L+F+++ETGSL LS+L VA TLQDG+SHY+
Sbjct: 348  GVALGSATQIAMFVVPLCVIIAWIMGINMDLNFSVIETGSLALSILAVALTLQDGSSHYL 407

Query: 1360 KGFVLLLCYVVIA 1398
            KG VLLLCY++IA
Sbjct: 408  KGLVLLLCYIIIA 420



 Score =  226 bits (575), Expect(2) = e-138
 Identities = 117/195 (60%), Positives = 138/195 (70%), Gaps = 2/195 (1%)
 Frame = +3

Query: 141 STTREPWLMENGNVKVLTRE--RHGRTAQNXXXXXXXXXXXXXXVSKVPISCLRKVLSNI 314
           S   +PWL+ENG +K LT+E  R GRTA N              VSKVP + LR+ L+N+
Sbjct: 3   SNPDQPWLLENGTLKGLTKETGRTGRTAHNMSSSSLRKKSDRTLVSKVPCALLRQFLANL 62

Query: 315 QEVILGTKLSVLFPAIPLAIIAHYCGFADPWIFGLSLLGLTPLAERISFLTEELTFYTGP 494
           QEV+LGTKL VLFPAIPLAI A   GFA PW+F LSL+GL PLAER+SFLTE++ +YTGP
Sbjct: 63  QEVLLGTKLFVLFPAIPLAIAAQCFGFARPWVFALSLIGLMPLAERVSFLTEQIAYYTGP 122

Query: 495 TVGGLLNATCGNATELIIALFALWQHKVDVVKYXXXXXXXXXXXXXXXXXXFCGGLANLH 674
           TVGGLLNATCGNATELIIA+FAL QHKV VVKY                  FCGG+AN+ 
Sbjct: 123 TVGGLLNATCGNATELIIAIFALAQHKVAVVKYSLLGSILSNLLLVLGTSLFCGGIANMK 182

Query: 675 SEQKYDRKQADVNTV 719
            EQKYDR+QADVN++
Sbjct: 183 KEQKYDRRQADVNSL 197


>ref|XP_004148618.1| PREDICTED: vacuolar cation/proton exchanger 1-like [Cucumis sativus]
            gi|449518469|ref|XP_004166264.1| PREDICTED: vacuolar
            cation/proton exchanger 1-like [Cucumis sativus]
            gi|402797827|gb|AFQ99295.1| cation exchanger CAX3
            [Cucumis sativus]
          Length = 447

 Score =  286 bits (732), Expect(2) = e-136
 Identities = 140/201 (69%), Positives = 171/201 (85%)
 Frame = +1

Query: 796  ASAYSKSDTLQLSRVSSIIMLVAYIAYLVFQLWTHREFFEAQXXXXXXLVSEEAPALGFW 975
            +++ + + TL LSR SSI+ML AYIAYLVFQLWTHR+ FEA+      +  EEA  +GFW
Sbjct: 218  SASTAAASTLHLSRASSIVMLTAYIAYLVFQLWTHRQLFEAEEGEDDDVPGEEA-VIGFW 276

Query: 976  SAFIWLVGMTVVIAFLSEYVVGTIEQAAATWGISVSFISIILLPIVGNAAEHAGAVIFAF 1155
            SAF WLVGMT+VIA LSEYVV TIE+A+ TWG+SVSF+SIILLPIVGNAAEHAGA+IFAF
Sbjct: 277  SAFAWLVGMTLVIALLSEYVVNTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAF 336

Query: 1156 KNKLDITLGVALGSATQIALFVVPVSVTFAWILDINMNLDFNLLETGSLVLSVLVVAFTL 1335
            KNKLDI+LGVALGSATQIALFV+P+ V  AW + ++M+L+FNL+ETGSL L+++  AFTL
Sbjct: 337  KNKLDISLGVALGSATQIALFVIPMCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTL 396

Query: 1336 QDGTSHYMKGFVLLLCYVVIA 1398
            QDGTSHY+KG VLLLCY++IA
Sbjct: 397  QDGTSHYIKGLVLLLCYIIIA 417



 Score =  228 bits (582), Expect(2) = e-136
 Identities = 116/192 (60%), Positives = 134/192 (69%), Gaps = 3/192 (1%)
 Frame = +3

Query: 153 EPWLMENGNVKVLTRER---HGRTAQNXXXXXXXXXXXXXXVSKVPISCLRKVLSNIQEV 323
           EPWLMENG +K LT+E    HGRTA N              VSK+   CLRK   N+QEV
Sbjct: 6   EPWLMENGTLKRLTKEHRHGHGRTAHNMSSSSLRKKSDLTLVSKIQFGCLRKFCINLQEV 65

Query: 324 ILGTKLSVLFPAIPLAIIAHYCGFADPWIFGLSLLGLTPLAERISFLTEELTFYTGPTVG 503
           ILGTKLS+LFPAIPLAI+A   GF  PWIF LSLLGLTPLAER+SFLTE++ ++TGPTVG
Sbjct: 66  ILGTKLSILFPAIPLAIVAQNFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFTGPTVG 125

Query: 504 GLLNATCGNATELIIALFALWQHKVDVVKYXXXXXXXXXXXXXXXXXXFCGGLANLHSEQ 683
           GLLNATCGNATELIIA+ AL Q K+DVVKY                  FCGG+AN+  EQ
Sbjct: 126 GLLNATCGNATELIIAILALTQRKIDVVKYSLLGSVLSNLLLVLGTSLFCGGIANIRREQ 185

Query: 684 KYDRKQADVNTV 719
            YDR+QADVN++
Sbjct: 186 NYDRRQADVNSL 197


>ref|XP_002274791.2| PREDICTED: vacuolar cation/proton exchanger 3-like [Vitis vinifera]
          Length = 448

 Score =  280 bits (716), Expect(2) = e-135
 Identities = 142/200 (71%), Positives = 165/200 (82%), Gaps = 1/200 (0%)
 Frame = +1

Query: 799  SAYSKSDTLQLSRVSSIIMLVAYIAYLVFQLWTHREFFEAQXXXXXXLVS-EEAPALGFW 975
            +A     TL LSR  SI+MLVAY+AYLVFQLWTHR+ FEA        VS +E P +GFW
Sbjct: 218  AALIADSTLSLSRAGSIVMLVAYLAYLVFQLWTHRKLFEAPEDGDDDTVSSDEEPVIGFW 277

Query: 976  SAFIWLVGMTVVIAFLSEYVVGTIEQAAATWGISVSFISIILLPIVGNAAEHAGAVIFAF 1155
            S+ +WL+ MT +IA LSE+VVGTIE A+ +WGISVSFISIILLPIVGNAAEHAGAVIFAF
Sbjct: 278  SSVVWLILMTAIIALLSEFVVGTIEVASESWGISVSFISIILLPIVGNAAEHAGAVIFAF 337

Query: 1156 KNKLDITLGVALGSATQIALFVVPVSVTFAWILDINMNLDFNLLETGSLVLSVLVVAFTL 1335
            KNKLDI+LGVALGSATQIA+FVVP+ V  AWI+ I M+LDF+LLET SL L+++V AFTL
Sbjct: 338  KNKLDISLGVALGSATQIAMFVVPLCVLVAWIMGIRMDLDFSLLETSSLALAIIVTAFTL 397

Query: 1336 QDGTSHYMKGFVLLLCYVVI 1395
            QDG+SHYMKG VLLLCYVVI
Sbjct: 398  QDGSSHYMKGLVLLLCYVVI 417



 Score =  231 bits (589), Expect(2) = e-135
 Identities = 116/192 (60%), Positives = 142/192 (73%), Gaps = 1/192 (0%)
 Frame = +3

Query: 147 TREPWLMENGNVKVLTRE-RHGRTAQNXXXXXXXXXXXXXXVSKVPISCLRKVLSNIQEV 323
           ++EPW MENGN+K  ++E +HGRTA N              V+KVP   +R++L+N+QEV
Sbjct: 3   SQEPWQMENGNLKGSSKEIKHGRTAHNMSSSSLRKKSDLSLVNKVPCGLVRQLLANLQEV 62

Query: 324 ILGTKLSVLFPAIPLAIIAHYCGFADPWIFGLSLLGLTPLAERISFLTEELTFYTGPTVG 503
           ILGTKLSVLFPAIPLAI+A Y GFA PWIFGLSLLGLTPLAER+ FLTE++ +YTGPTVG
Sbjct: 63  ILGTKLSVLFPAIPLAIVAQYYGFAGPWIFGLSLLGLTPLAERLGFLTEQIAYYTGPTVG 122

Query: 504 GLLNATCGNATELIIALFALWQHKVDVVKYXXXXXXXXXXXXXXXXXXFCGGLANLHSEQ 683
           GLLNATCGNATELIIA+FAL ++K+ VVKY                  FCGG+ NL  EQ
Sbjct: 123 GLLNATCGNATELIIAIFALSKNKILVVKYSLLGSVLSNLLLVLGTSLFCGGIYNLREEQ 182

Query: 684 KYDRKQADVNTV 719
           KYDRKQ+D+N++
Sbjct: 183 KYDRKQSDINSL 194


>gb|AET87472.1| Ca2+/H+ exchanging protein [Morus alba var. multicaulis]
            gi|373254664|gb|AEY68239.1| calcium exchanger protein 1
            [Morus alba]
          Length = 447

 Score =  280 bits (715), Expect(2) = e-135
 Identities = 138/200 (69%), Positives = 163/200 (81%)
 Frame = +1

Query: 796  ASAYSKSDTLQLSRVSSIIMLVAYIAYLVFQLWTHREFFEAQXXXXXXLVSEEAPALGFW 975
            A   +  +TLQLSR SSI+ML+AY AYLVFQLWTHR+ FEA+          E   +GFW
Sbjct: 215  AGVSTVDETLQLSRASSIVMLLAYAAYLVFQLWTHRQLFEAEEESGEGGDGSEEAVIGFW 274

Query: 976  SAFIWLVGMTVVIAFLSEYVVGTIEQAAATWGISVSFISIILLPIVGNAAEHAGAVIFAF 1155
            S F WLVGMT+VIA LSEYVV TIE A+ +WG+S+SF+SIILLPIVGNAAEHAGA+IFAF
Sbjct: 275  SGFAWLVGMTLVIALLSEYVVETIEDASDSWGLSISFLSIILLPIVGNAAEHAGAIIFAF 334

Query: 1156 KNKLDITLGVALGSATQIALFVVPVSVTFAWILDINMNLDFNLLETGSLVLSVLVVAFTL 1335
            KNKLDITLGVALGSA+QIA+FVVP+ V  +WI+  NM+L+FNLLET SL L+++  AFTL
Sbjct: 335  KNKLDITLGVALGSASQIAMFVVPLCVVVSWIMGNNMDLNFNLLETSSLALAIIATAFTL 394

Query: 1336 QDGTSHYMKGFVLLLCYVVI 1395
            QDGTSHYMKG VLLLCY+VI
Sbjct: 395  QDGTSHYMKGLVLLLCYIVI 414



 Score =  231 bits (589), Expect(2) = e-135
 Identities = 118/194 (60%), Positives = 139/194 (71%), Gaps = 1/194 (0%)
 Frame = +3

Query: 141 STTREPWLMENGNVKVLTRE-RHGRTAQNXXXXXXXXXXXXXXVSKVPISCLRKVLSNIQ 317
           S   +PWL+ENGN K L++E RHGRTA N              VSKVP   LRK+L+N+Q
Sbjct: 3   SNLDQPWLLENGNPKGLSKEMRHGRTAHNMSSSSLRKKSDLKLVSKVPCGLLRKILANLQ 62

Query: 318 EVILGTKLSVLFPAIPLAIIAHYCGFADPWIFGLSLLGLTPLAERISFLTEELTFYTGPT 497
           EVILGTKLS+LFPAIPLAI A   GF  PW+F LS+LGLTPLAER+SFLTE++ +YTGPT
Sbjct: 63  EVILGTKLSILFPAIPLAIAAECYGFGRPWVFALSMLGLTPLAERVSFLTEQIAYYTGPT 122

Query: 498 VGGLLNATCGNATELIIALFALWQHKVDVVKYXXXXXXXXXXXXXXXXXXFCGGLANLHS 677
           VGGLLNATCGNATELIIA+FAL + K+DVVKY                   CGG+ANL  
Sbjct: 123 VGGLLNATCGNATELIIAIFALSEGKIDVVKYSLLGSILSNLLLVLGTSLLCGGIANLGR 182

Query: 678 EQKYDRKQADVNTV 719
           EQKYDR+QADVN++
Sbjct: 183 EQKYDRRQADVNSL 196


>ref|XP_002528100.1| Vacuolar cation/proton exchanger 1a, putative [Ricinus communis]
            gi|223532489|gb|EEF34279.1| Vacuolar cation/proton
            exchanger 1a, putative [Ricinus communis]
          Length = 449

 Score =  275 bits (702), Expect(2) = e-135
 Identities = 136/192 (70%), Positives = 159/192 (82%)
 Frame = +1

Query: 820  TLQLSRVSSIIMLVAYIAYLVFQLWTHREFFEAQXXXXXXLVSEEAPALGFWSAFIWLVG 999
            TL+LSR SS++MLVAYIAY+ FQL THR+ FEA          EE P +GFWS   WLVG
Sbjct: 228  TLELSRASSLVMLVAYIAYIFFQLVTHRQLFEAPEESQDGDEVEETPVIGFWSGIAWLVG 287

Query: 1000 MTVVIAFLSEYVVGTIEQAAATWGISVSFISIILLPIVGNAAEHAGAVIFAFKNKLDITL 1179
            MT VIA LSEYVVGTIE A+ +WG+SVSFISIILLPIVGNAAEHAGA+IFAFKNKLDI+L
Sbjct: 288  MTAVIALLSEYVVGTIEDASDSWGLSVSFISIILLPIVGNAAEHAGAIIFAFKNKLDISL 347

Query: 1180 GVALGSATQIALFVVPVSVTFAWILDINMNLDFNLLETGSLVLSVLVVAFTLQDGTSHYM 1359
            GVALGSATQI++FVVP+ V  +WI+ I M+L+FNLLETG+L LS++  AFTLQDGTSHY+
Sbjct: 348  GVALGSATQISMFVVPLCVIVSWIIGIKMDLNFNLLETGTLALSIIATAFTLQDGTSHYL 407

Query: 1360 KGFVLLLCYVVI 1395
            KG  LLLCY+VI
Sbjct: 408  KGLSLLLCYIVI 419



 Score =  234 bits (597), Expect(2) = e-135
 Identities = 123/199 (61%), Positives = 146/199 (73%), Gaps = 2/199 (1%)
 Frame = +3

Query: 129 MATDS-TTREPWLMENGNVKVLTRE-RHGRTAQNXXXXXXXXXXXXXXVSKVPISCLRKV 302
           MA++S + +EPWL+E+GN+K L++E RHGRTA N              VSK+    LR +
Sbjct: 1   MASNSHSLQEPWLLEHGNLKGLSKEMRHGRTAHNMSSSSLRKKSDLTLVSKLRCGLLRNL 60

Query: 303 LSNIQEVILGTKLSVLFPAIPLAIIAHYCGFADPWIFGLSLLGLTPLAERISFLTEELTF 482
           L+N+QEVILGTKLSVLFPAIPLAI+A   GF  PWIF LSLLGLTPLAER+SFLTE++ +
Sbjct: 61  LANLQEVILGTKLSVLFPAIPLAIVAQCYGFGRPWIFALSLLGLTPLAERVSFLTEQIAY 120

Query: 483 YTGPTVGGLLNATCGNATELIIALFALWQHKVDVVKYXXXXXXXXXXXXXXXXXXFCGGL 662
           +TGPTVGGLLNATCGNATELIIA+FAL QHK+ VVKY                  FCGG+
Sbjct: 121 FTGPTVGGLLNATCGNATELIIAIFALSQHKIAVVKYSLLGSILSNLLLVLGTSLFCGGI 180

Query: 663 ANLHSEQKYDRKQADVNTV 719
           ANL  EQKYDRKQADVNT+
Sbjct: 181 ANLGQEQKYDRKQADVNTM 199