BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00000343
         (837 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002509816.1| short-chain dehydrogenase, putative [Ricinus...   370   e-100
ref|XP_002270104.1| PREDICTED: short-chain dehydrogenase TIC 32,...   369   e-100
ref|NP_194073.2| Rossmann-fold NAD(P)-binding domain-containing ...   368   e-100
ref|XP_002272242.2| PREDICTED: short-chain dehydrogenase TIC 32,...   368   1e-99
ref|XP_002869780.1| short-chain dehydrogenase/reductase family p...   367   2e-99

>ref|XP_002509816.1| short-chain dehydrogenase, putative [Ricinus communis]
           gi|223549715|gb|EEF51203.1| short-chain dehydrogenase,
           putative [Ricinus communis]
          Length = 315

 Score =  370 bits (951), Expect = e-100
 Identities = 184/267 (68%), Positives = 222/267 (83%), Gaps = 1/267 (0%)
 Frame = +1

Query: 40  MWLLGFNGPSGFSARSTAEQVTKGIDGTGLTAIVTGASNGIGIETARVLALRGVHVIMGV 219
           MW+ G+ GPSGFSA STAEQVT+GIDGTGLTAIVTGAS+GIG+ETARVLALR VHV+M V
Sbjct: 1   MWIFGWKGPSGFSANSTAEQVTQGIDGTGLTAIVTGASSGIGVETARVLALRDVHVVMAV 60

Query: 220 RNVKAGNKVKDDILKKIPNAKIDVMEIDLNSQASIRKFASEYISSGLPLNILVNNAGIMA 399
           RNV AG  VK+ ILK+IP AKIDVM+ DL+S AS+RKFASEYISSGLPLN+L+NNAG+MA
Sbjct: 61  RNVDAGRNVKESILKEIPTAKIDVMQFDLSSMASVRKFASEYISSGLPLNLLINNAGVMA 120

Query: 400 PPFTLSKDNIEQQFAVNHLGPFLLTNLLLETMKDTARKTQKEGRIVNVSSVLHHNGYKEG 579
            PF LS+DNIE+QFA NH+G FLLT+LLLETMK+TAR++ +EGRIVNVSS  H   Y+EG
Sbjct: 121 TPFMLSQDNIERQFATNHVGHFLLTDLLLETMKNTARESSREGRIVNVSSAGHRFTYREG 180

Query: 580 IRFDKINDAASYNGNAAYGQSKLCNLLHTNELAKRLKREGVNITANSVHPGIIATNITNN 759
           IRFDK+ND A YN   AYGQSKL N+LH  ELA+RLK +GV+I+ NS+HPG I TN+   
Sbjct: 181 IRFDKLNDEAGYNSILAYGQSKLANILHAGELARRLKEDGVDISVNSLHPGAIDTNLLRY 240

Query: 760 LGLLGWALN-IGKYLLKNVAQGAATTC 837
             ++   ++ + KY++KNV QGAATTC
Sbjct: 241 HSVINGIVSLVAKYVIKNVQQGAATTC 267


>ref|XP_002270104.1| PREDICTED: short-chain dehydrogenase TIC 32, chloroplastic [Vitis
           vinifera] gi|302143828|emb|CBI22689.3| unnamed protein
           product [Vitis vinifera]
          Length = 313

 Score =  369 bits (947), Expect = e-100
 Identities = 186/267 (69%), Positives = 219/267 (82%), Gaps = 1/267 (0%)
 Frame = +1

Query: 40  MWLLGFNGPSGFSARSTAEQVTKGIDGTGLTAIVTGASNGIGIETARVLALRGVHVIMGV 219
           MW     GPSGFSA STAE+VT+GIDGTGLTAIVTGAS+GIG ET RVLALRGVHV+MGV
Sbjct: 1   MWWFSRKGPSGFSASSTAEEVTQGIDGTGLTAIVTGASSGIGTETTRVLALRGVHVVMGV 60

Query: 220 RNVKAGNKVKDDILKKIPNAKIDVMEIDLNSQASIRKFASEYISSGLPLNILVNNAGIMA 399
           RN+ AG +VK+ I+K+IP AK+D ME+DL+S AS+RKFASEY SSGLPLNIL+NNAGIMA
Sbjct: 61  RNMSAGKEVKEAIVKEIPTAKVDAMELDLSSMASVRKFASEYNSSGLPLNILINNAGIMA 120

Query: 400 PPFTLSKDNIEQQFAVNHLGPFLLTNLLLETMKDTARKTQKEGRIVNVSSVLHHNGYKEG 579
            P+ LSKDNIE QFA NHLG FLLTNLLL+TMK T RK++KEGRIVNVSS+ H   Y+EG
Sbjct: 121 VPYMLSKDNIEMQFATNHLGHFLLTNLLLDTMKKTTRKSRKEGRIVNVSSMAHRYPYREG 180

Query: 580 IRFDKINDAASYNGNAAYGQSKLCNLLHTNELAKRLKREGVNITANSVHPGIIATNITNN 759
           IRFDKIND + Y+   AYGQSKL N+LH NELA+R K +GV+ITANS+HPG I TN+   
Sbjct: 181 IRFDKINDKSGYSSLFAYGQSKLANVLHANELARRFKEDGVDITANSLHPGAIVTNLFRC 240

Query: 760 LGLLGWALN-IGKYLLKNVAQGAATTC 837
             ++   +N +GK +LKNV QGAATTC
Sbjct: 241 SSIVSGLVNTVGKLVLKNVQQGAATTC 267


>ref|NP_194073.2| Rossmann-fold NAD(P)-binding domain-containing protein [Arabidopsis
           thaliana] gi|42572999|ref|NP_974596.1| Rossmann-fold
           NAD(P)-binding domain-containing protein [Arabidopsis
           thaliana] gi|23297050|gb|AAN13078.1| unknown protein
           [Arabidopsis thaliana] gi|51970714|dbj|BAD44049.1|
           unknown protein [Arabidopsis thaliana]
           gi|332659350|gb|AEE84750.1| Rossmann-fold NAD(P)-binding
           domain-containing protein [Arabidopsis thaliana]
           gi|332659351|gb|AEE84751.1| Rossmann-fold NAD(P)-binding
           domain-containing protein [Arabidopsis thaliana]
          Length = 316

 Score =  368 bits (945), Expect = e-100
 Identities = 184/268 (68%), Positives = 221/268 (82%), Gaps = 2/268 (0%)
 Frame = +1

Query: 40  MWLLGFNGPSGFSARSTAEQVTKGIDGTGLTAIVTGASNGIGIETARVLALRGVHVIMGV 219
           MW  G  G SGFS+RSTAE+VT G+DGTGLTAIVTGAS+GIG+ETARVLALRGVHV+M V
Sbjct: 1   MWFFGSKGASGFSSRSTAEEVTHGVDGTGLTAIVTGASSGIGVETARVLALRGVHVVMAV 60

Query: 220 RNVKAGNKVKDDILKKIPNAKIDVMEIDLNSQASIRKFASEYISSGLPLNILVNNAGIMA 399
           RN  AG KVK+DI+K++P AK+DVME++L+S  S+RKFASEY S+GLPLN+L+NNAGIMA
Sbjct: 61  RNTGAGAKVKEDIVKQVPGAKVDVMELELSSMESVRKFASEYKSAGLPLNLLINNAGIMA 120

Query: 400 PPFTLSKDNIEQQFAVNHLGPFLLTNLLLETMKDTARKTQKEGRIVNVSSVLHHNGYKEG 579
            PF LSKDNIE QFA NHLG FLLT LLL+TMK+T+R++++EGRIVNVSS  H   Y EG
Sbjct: 121 CPFMLSKDNIELQFATNHLGHFLLTKLLLDTMKNTSRESKREGRIVNVSSEAHRYSYPEG 180

Query: 580 IRFDKINDAASYNGNAAYGQSKLCNLLHTNELAKRLKREGVNITANSVHPGIIATNITN- 756
           +RFDKIND +SY+   AYGQSKLCN+LH NELAK+LK +GVNITANS+HPG I TN+   
Sbjct: 181 VRFDKINDESSYSSIRAYGQSKLCNVLHANELAKQLKEDGVNITANSLHPGAIMTNLWGY 240

Query: 757 -NLGLLGWALNIGKYLLKNVAQGAATTC 837
            N  L G    + KY++K+V QGAATTC
Sbjct: 241 FNSYLAGAVGAVAKYMVKSVPQGAATTC 268


>ref|XP_002272242.2| PREDICTED: short-chain dehydrogenase TIC 32, chloroplastic-like
           [Vitis vinifera] gi|147792549|emb|CAN65620.1|
           hypothetical protein VITISV_040852 [Vitis vinifera]
           gi|302143827|emb|CBI22688.3| unnamed protein product
           [Vitis vinifera]
          Length = 313

 Score =  368 bits (944), Expect = 1e-99
 Identities = 188/267 (70%), Positives = 220/267 (82%), Gaps = 1/267 (0%)
 Frame = +1

Query: 40  MWLLGFNGPSGFSARSTAEQVTKGIDGTGLTAIVTGASNGIGIETARVLALRGVHVIMGV 219
           MW  G NG SGFSA STA +VT GIDGTGLTAIVTGAS+GIG ETARVLALRGVHV+M V
Sbjct: 1   MWWFGRNGASGFSASSTAVEVTHGIDGTGLTAIVTGASSGIGTETARVLALRGVHVVMAV 60

Query: 220 RNVKAGNKVKDDILKKIPNAKIDVMEIDLNSQASIRKFASEYISSGLPLNILVNNAGIMA 399
           RN+ AG +VK+ I K+IP AKID ME+DL+S AS+RKFASE+ SSGLPLN+L+NNAG+MA
Sbjct: 61  RNMAAGREVKEAIAKEIPTAKIDAMELDLSSMASVRKFASEFSSSGLPLNLLINNAGLMA 120

Query: 400 PPFTLSKDNIEQQFAVNHLGPFLLTNLLLETMKDTARKTQKEGRIVNVSSVLHHNGYKEG 579
            PF LSKDNIE QFA NH+G FLLTNLLLETMK TAR++ KEGRIVNVSS  H   Y EG
Sbjct: 121 TPFMLSKDNIELQFATNHIGHFLLTNLLLETMKKTARESNKEGRIVNVSSRRHRFSYHEG 180

Query: 580 IRFDKINDAASYNGNAAYGQSKLCNLLHTNELAKRLKREGVNITANSVHPGIIATNITNN 759
           IRFD IND + YN  +AYGQSKL N+LH NEL++RLK +G NITANS+HPG IATN+  +
Sbjct: 181 IRFDMINDQSGYNRLSAYGQSKLANVLHANELSRRLKDDGANITANSLHPGAIATNLFRH 240

Query: 760 LGLLGWALNI-GKYLLKNVAQGAATTC 837
           + L+G  ++I GKY++KNV QGAATTC
Sbjct: 241 VPLVGGFIDIFGKYVVKNVQQGAATTC 267


>ref|XP_002869780.1| short-chain dehydrogenase/reductase family protein [Arabidopsis
           lyrata subsp. lyrata] gi|297315616|gb|EFH46039.1|
           short-chain dehydrogenase/reductase family protein
           [Arabidopsis lyrata subsp. lyrata]
          Length = 322

 Score =  367 bits (941), Expect = 2e-99
 Identities = 183/268 (68%), Positives = 221/268 (82%), Gaps = 2/268 (0%)
 Frame = +1

Query: 40  MWLLGFNGPSGFSARSTAEQVTKGIDGTGLTAIVTGASNGIGIETARVLALRGVHVIMGV 219
           MW  G  G SGFS+RSTAE+VT G+DGTGLTAI+TGAS+GIG+ETARVL+LRGVHV+M V
Sbjct: 1   MWFFGSKGASGFSSRSTAEEVTHGVDGTGLTAIITGASSGIGVETARVLSLRGVHVVMAV 60

Query: 220 RNVKAGNKVKDDILKKIPNAKIDVMEIDLNSQASIRKFASEYISSGLPLNILVNNAGIMA 399
           RN  +G KVK+DI+K++P AK+DVME+DL+S  S+RKFASEY S+GLPLN+L+NNAGIMA
Sbjct: 61  RNTGSGAKVKEDIVKQVPGAKLDVMELDLSSMESVRKFASEYKSAGLPLNLLINNAGIMA 120

Query: 400 PPFTLSKDNIEQQFAVNHLGPFLLTNLLLETMKDTARKTQKEGRIVNVSSVLHHNGYKEG 579
            PF LSKDNIE QFA NHLG FLLT LLL+TMK+T+R++++EGRIVN+SS  H   Y EG
Sbjct: 121 CPFMLSKDNIELQFATNHLGHFLLTKLLLDTMKNTSRESKREGRIVNLSSEAHWFSYPEG 180

Query: 580 IRFDKINDAASYNGNAAYGQSKLCNLLHTNELAKRLKREGVNITANSVHPGIIATNITN- 756
           +RFDKIND +SY+   AYGQSKLCN+LH NELAK+LK +GVNITANSVHPG I TN+   
Sbjct: 181 VRFDKINDKSSYSSMRAYGQSKLCNVLHANELAKQLKEDGVNITANSVHPGAIMTNLGRY 240

Query: 757 -NLGLLGWALNIGKYLLKNVAQGAATTC 837
            N  L G    + KY+LK+V QGAATTC
Sbjct: 241 FNPYLAGAVGAVAKYILKSVPQGAATTC 268