BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00000341
         (2977 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002283993.1| PREDICTED: zinc-metallopeptidase, peroxisoma...  1386   0.0  
ref|XP_002532073.1| Insulin-degrading enzyme, putative [Ricinus ...  1371   0.0  
ref|NP_001233926.1| insulin degrading enzyme [Solanum lycopersic...  1365   0.0  
ref|XP_002308028.1| predicted protein [Populus trichocarpa] gi|2...  1330   0.0  
ref|XP_002283970.1| PREDICTED: zinc-metallopeptidase, peroxisoma...  1322   0.0  

>ref|XP_002283993.1| PREDICTED: zinc-metallopeptidase, peroxisomal [Vitis vinifera]
            gi|297739661|emb|CBI29843.3| unnamed protein product
            [Vitis vinifera]
          Length = 965

 Score = 1386 bits (3588), Expect = 0.0
 Identities = 659/842 (78%), Positives = 741/842 (88%)
 Frame = +1

Query: 4    REYRNIILHNSLQVLLISDPETDKCAASMNVCVGSFSDPKGLEGLAHFLEHMLFYASEKY 183
            REYR I+L NSL+VLLISDP+TDK AASM+V VGSF DP+G  GLAHFLEHMLFYASEKY
Sbjct: 16   REYRRIVLRNSLEVLLISDPDTDKAAASMSVSVGSFCDPEGFPGLAHFLEHMLFYASEKY 75

Query: 184  PLEDSYSKYITEHGGSTNAFTSSEQTNFYFDVNADCFEEALDRFAQFFIKPLMSSDATTR 363
            PLEDSYSKYI EHGGSTNAFTSSE TN+YFDVN+DCFEEALDRFAQFF+KPLMS+DATTR
Sbjct: 76   PLEDSYSKYIIEHGGSTNAFTSSEHTNYYFDVNSDCFEEALDRFAQFFVKPLMSADATTR 135

Query: 364  EIKAVDSENQKNLLSDAWRMNQLQKHLSAEDHPYHKFSTGNWDTLEVRPKSKGVDTRQEL 543
            EIKAVDSENQKNLLSDAWRM QLQKH+SAE HPYHKFSTGNWDTLEV+PK KG+DTR EL
Sbjct: 136  EIKAVDSENQKNLLSDAWRMCQLQKHISAEGHPYHKFSTGNWDTLEVKPKEKGLDTRHEL 195

Query: 544  LKFYEQNYSSNLMCLVVYGKESLDKIGSQVVDKFQDISNRDRSSLHFPGQPCMPEHLQIL 723
            +KFYE++YS+NLM LVVY KESLDKI S V  KFQ+I N+DRS+   PGQPC  EHLQIL
Sbjct: 196  IKFYEEHYSANLMHLVVYTKESLDKIQSLVEHKFQEIQNKDRSNFQIPGQPCTSEHLQIL 255

Query: 724  VKTVPIKQGHKLRFTWPVTPGILHYKEGPSRYLGHLIGHEGEGSLFYALKKLGWATSLSA 903
            VKTVPIKQGHKLR  WP+TP I +YKEGP RYLGHLIGHEGEGSLFY LK LGWATSLSA
Sbjct: 256  VKTVPIKQGHKLRVIWPITPSIHNYKEGPCRYLGHLIGHEGEGSLFYILKTLGWATSLSA 315

Query: 904  GESDETREFSFFKVNIDLTDAGHEHFEDIVALLFKYIRVLQQSGVCKWIFDELSAICETA 1083
            GE D T EFSFFKV IDLT+AGHEH +DIV LLFKYI +LQQ+GVCKWIFDELSAICET 
Sbjct: 316  GEGDWTCEFSFFKVVIDLTEAGHEHMQDIVGLLFKYISLLQQTGVCKWIFDELSAICETV 375

Query: 1084 FHYQDKIRPMDYVVNVASNMQLYPTRDWLVGSSLPSTFNPDIIQSILNELSPDSVRIFWS 1263
            FHYQDKI P+DYVVNV+SNM+LYP +DWLVGSSLPS F+PD+IQ +L+EL+P++VRIFW 
Sbjct: 376  FHYQDKIPPIDYVVNVSSNMELYPPKDWLVGSSLPSKFSPDVIQKVLDELAPNNVRIFWE 435

Query: 1264 SMKFEGQTDKTEPWYGTAYSVEKMTRSLIEKWIERAPEEHLHLPTQNVFIPTDLTLKTAP 1443
            S  FEG TD  EPWYGTAYS+EK+T S+I++W+  AP EHLHLP+ NVFIPTDL+LK   
Sbjct: 436  SKNFEGHTDMVEPWYGTAYSIEKITSSMIQQWMLAAPNEHLHLPSPNVFIPTDLSLKDVQ 495

Query: 1444 EKANLPLLLRKSPYSRLWYKSDSTFFTPKAYVKIDFNCPFAGNSPEAEVLTDIFTRLVTD 1623
            EKA  P+LLRKS YS LWYK D+ F TPKAYVKIDFNCPFA +SPEA+VLTDIFTRL+ D
Sbjct: 496  EKAKFPVLLRKSSYSTLWYKPDTMFSTPKAYVKIDFNCPFASSSPEADVLTDIFTRLLMD 555

Query: 1624 YLNEYAYYAQVAGLYYAVNHTDNGFQVTIVGYNHKLKILLDTVIEKIANFEVKPDRFSVI 1803
            YLNEYAYYAQVAGLYY +NHTD+GFQV + GYNHKL+ILL+TV+EKIANF+VKPDRF VI
Sbjct: 556  YLNEYAYYAQVAGLYYGINHTDSGFQVAVTGYNHKLRILLETVVEKIANFKVKPDRFLVI 615

Query: 1804 KELITKDYKNFKFQQPYQQAMYYCSLILQDQTWPWSDQLEALPHLTVDHLAKFYPLMISK 1983
            KE++TK+Y+NFKFQQPYQQAMYYCSLILQD TWPW D LE +PHL  D LAKF P+++S+
Sbjct: 616  KEMVTKEYQNFKFQQPYQQAMYYCSLILQDNTWPWMDGLEVIPHLEADDLAKFVPMLLSR 675

Query: 1984 TFIECYVAGNIEPKEAELIIQHVETVFYKGQDPKSKALFPSQHLTNRVVKLDRGTNYFYT 2163
             F++CY+AGNIEPKEAE +I H+E +FY G  P S+ LFPSQ+LTNRV+KLDRG +YFY 
Sbjct: 676  AFLDCYIAGNIEPKEAESMIHHIEDIFYSGPHPISQPLFPSQYLTNRVIKLDRGMSYFYP 735

Query: 2164 AEGLNSSDENSAIVHYIQVHQDDYMLNVKLQLFALVAKQPAFHQLRSVEQLGYITVLMQR 2343
            AEGLN SDENSA+VHYIQVH+DD++ NVKLQLFAL+AKQ AFHQLRSVEQLGYITVLMQR
Sbjct: 736  AEGLNPSDENSALVHYIQVHRDDFLPNVKLQLFALIAKQRAFHQLRSVEQLGYITVLMQR 795

Query: 2344 NDSGVRGVQFIIQSTAKGPRHIDLRVESFLKMFESKLQEMSNEEFKSNVNALIDMKLEKH 2523
            NDSG+RGVQFIIQST KGP HID RV  FLKMFESKL  MS +EFKSNVNALIDMKLEKH
Sbjct: 796  NDSGIRGVQFIIQSTVKGPGHIDSRVVEFLKMFESKLYAMSEDEFKSNVNALIDMKLEKH 855

Query: 2524 KN 2529
            KN
Sbjct: 856  KN 857



 Score =  135 bits (339), Expect = 8e-29
 Identities = 63/89 (70%), Positives = 78/89 (87%)
 Frame = +2

Query: 2621 DRREREVAALKQVTHKDLIDFFNEHIKVGAPQKKSLSVRVYGSSHSSEYEEDKKESLEPN 2800
            DRRE EVAALK++T K+LIDFFNEHIKVGAPQKK+LSVRVYG  H+SEY ++KKE+ +P 
Sbjct: 877  DRREAEVAALKKLTQKELIDFFNEHIKVGAPQKKTLSVRVYGGLHTSEYADEKKEANQPK 936

Query: 2801 YVQIEDIFSFRRSRPLYSSFKGGVGHMKL 2887
             V+I+DIF FR+S+PLY SFKGG+G +KL
Sbjct: 937  QVKIDDIFKFRKSQPLYGSFKGGLGQVKL 965


>ref|XP_002532073.1| Insulin-degrading enzyme, putative [Ricinus communis]
            gi|223528255|gb|EEF30307.1| Insulin-degrading enzyme,
            putative [Ricinus communis]
          Length = 967

 Score = 1371 bits (3548), Expect = 0.0
 Identities = 653/843 (77%), Positives = 740/843 (87%)
 Frame = +1

Query: 1    KREYRNIILHNSLQVLLISDPETDKCAASMNVCVGSFSDPKGLEGLAHFLEHMLFYASEK 180
            KREYR I+L NSL+VLLISDPETDKCAASM+V VG FSDP GLEGLAHFLEHMLFYASEK
Sbjct: 17   KREYRRIVLRNSLEVLLISDPETDKCAASMDVSVGFFSDPAGLEGLAHFLEHMLFYASEK 76

Query: 181  YPLEDSYSKYITEHGGSTNAFTSSEQTNFYFDVNADCFEEALDRFAQFFIKPLMSSDATT 360
            YPLEDSYSKYITEHGGSTNAFTSSE TN+YFDVN DCFE+ALDRFAQFFIKPLMS+DAT 
Sbjct: 77   YPLEDSYSKYITEHGGSTNAFTSSEFTNYYFDVNTDCFEDALDRFAQFFIKPLMSADATM 136

Query: 361  REIKAVDSENQKNLLSDAWRMNQLQKHLSAEDHPYHKFSTGNWDTLEVRPKSKGVDTRQE 540
            REIKAVDSENQKNLLSDAWRM QLQKHLS E HPYHKF TGNWDTLEVRPK+KG+DTR E
Sbjct: 137  REIKAVDSENQKNLLSDAWRMGQLQKHLSDEGHPYHKFGTGNWDTLEVRPKAKGLDTRNE 196

Query: 541  LLKFYEQNYSSNLMCLVVYGKESLDKIGSQVVDKFQDISNRDRSSLHFPGQPCMPEHLQI 720
            L+KFYE+NYS+N M LV+Y KESLDK+   + DKFQ I N+DRS L FPGQPC  EHLQI
Sbjct: 197  LIKFYEENYSANRMHLVIYAKESLDKLQILIEDKFQHIRNKDRSCLSFPGQPCSSEHLQI 256

Query: 721  LVKTVPIKQGHKLRFTWPVTPGILHYKEGPSRYLGHLIGHEGEGSLFYALKKLGWATSLS 900
            LVK VPIKQGH+L+  WP+TP ILHYKEGP RYLGHLIGHEGEGSLFY LK LGWATSLS
Sbjct: 257  LVKAVPIKQGHRLKIIWPITPEILHYKEGPCRYLGHLIGHEGEGSLFYVLKTLGWATSLS 316

Query: 901  AGESDETREFSFFKVNIDLTDAGHEHFEDIVALLFKYIRVLQQSGVCKWIFDELSAICET 1080
            AGE D T EFSFFKV IDLTDAGHEH +DI+ LLFKYI +LQQSGV +WIF+EL+A+CET
Sbjct: 317  AGEGDWTMEFSFFKVGIDLTDAGHEHMQDIIGLLFKYIHLLQQSGVSEWIFNELAAVCET 376

Query: 1081 AFHYQDKIRPMDYVVNVASNMQLYPTRDWLVGSSLPSTFNPDIIQSILNELSPDSVRIFW 1260
            +FHYQDKI P+DYVV +A NM +YP +DWLVGSSLPS F+PDIIQ +L++LSP+SVRIFW
Sbjct: 377  SFHYQDKIPPIDYVVTIACNMNIYPPKDWLVGSSLPSNFSPDIIQMVLHQLSPNSVRIFW 436

Query: 1261 SSMKFEGQTDKTEPWYGTAYSVEKMTRSLIEKWIERAPEEHLHLPTQNVFIPTDLTLKTA 1440
             S  FEGQT+K EPWYGTAYSVEK+   +I++W+  AP+E+LHLP  NVFIPTDL+LK+A
Sbjct: 437  ESKNFEGQTEKVEPWYGTAYSVEKIDSLVIQEWMLSAPDENLHLPAPNVFIPTDLSLKSA 496

Query: 1441 PEKANLPLLLRKSPYSRLWYKSDSTFFTPKAYVKIDFNCPFAGNSPEAEVLTDIFTRLVT 1620
             EK  LP+LLRKS YS LWYK D+ F TPKAYVKIDF+CP AG+SPEA+VLTDIF RL+ 
Sbjct: 497  QEKVILPVLLRKSSYSSLWYKPDTMFNTPKAYVKIDFSCPHAGSSPEADVLTDIFARLLM 556

Query: 1621 DYLNEYAYYAQVAGLYYAVNHTDNGFQVTIVGYNHKLKILLDTVIEKIANFEVKPDRFSV 1800
            DYLNEYAYYAQVAGLYY +  TD+GFQVT+VGYNHKLKILL+TVIEKIA F+V PDRFSV
Sbjct: 557  DYLNEYAYYAQVAGLYYGITKTDSGFQVTLVGYNHKLKILLETVIEKIAKFKVNPDRFSV 616

Query: 1801 IKELITKDYKNFKFQQPYQQAMYYCSLILQDQTWPWSDQLEALPHLTVDHLAKFYPLMIS 1980
            IKE++ K YKNFKFQQPYQQA+YY SLILQ+Q WPW ++LE LPHL  + LAKF P+M+S
Sbjct: 617  IKEMVIKKYKNFKFQQPYQQAIYYSSLILQNQAWPWMEELEVLPHLVAEDLAKFVPIMLS 676

Query: 1981 KTFIECYVAGNIEPKEAELIIQHVETVFYKGQDPKSKALFPSQHLTNRVVKLDRGTNYFY 2160
            ++F+ECY+AGNIE  EAE II+H+E VF+KGQ+P  + LFPSQHLTNRV+KL RG +YFY
Sbjct: 677  RSFLECYIAGNIESIEAESIIEHIENVFFKGQNPICQPLFPSQHLTNRVMKLGRGKSYFY 736

Query: 2161 TAEGLNSSDENSAIVHYIQVHQDDYMLNVKLQLFALVAKQPAFHQLRSVEQLGYITVLMQ 2340
              EGLN SDENSA+VHYIQVHQDD++LNVKLQLFAL+AKQPAFHQLRSVEQLGYITVLM 
Sbjct: 737  AIEGLNPSDENSALVHYIQVHQDDFLLNVKLQLFALIAKQPAFHQLRSVEQLGYITVLMP 796

Query: 2341 RNDSGVRGVQFIIQSTAKGPRHIDLRVESFLKMFESKLQEMSNEEFKSNVNALIDMKLEK 2520
            RNDSG+RGV FIIQST KGP HIDLRVE+FLK FE+KL EM+N+EFK+NVN+LIDMKLEK
Sbjct: 797  RNDSGIRGVHFIIQSTVKGPVHIDLRVEAFLKSFETKLYEMTNDEFKNNVNSLIDMKLEK 856

Query: 2521 HKN 2529
            HKN
Sbjct: 857  HKN 859



 Score =  127 bits (319), Expect = 2e-26
 Identities = 58/89 (65%), Positives = 76/89 (85%)
 Frame = +2

Query: 2621 DRREREVAALKQVTHKDLIDFFNEHIKVGAPQKKSLSVRVYGSSHSSEYEEDKKESLEPN 2800
            DRR+ EVAAL+Q+T ++ +DFFNE+IKVGAP +++LS+RVYG+SHS+EY  DK ESL PN
Sbjct: 879  DRRDSEVAALRQLTQQEFVDFFNENIKVGAPGRRTLSIRVYGASHSAEYTSDKSESLLPN 938

Query: 2801 YVQIEDIFSFRRSRPLYSSFKGGVGHMKL 2887
             +QI+DIFSFRR++ LY S +GG GHMKL
Sbjct: 939  SIQIDDIFSFRRTQSLYGSCRGGFGHMKL 967


>ref|NP_001233926.1| insulin degrading enzyme [Solanum lycopersicum]
            gi|15485612|emb|CAC67408.1| insulin degrading enzyme
            [Solanum lycopersicum]
          Length = 971

 Score = 1365 bits (3532), Expect = 0.0
 Identities = 649/843 (76%), Positives = 740/843 (87%)
 Frame = +1

Query: 1    KREYRNIILHNSLQVLLISDPETDKCAASMNVCVGSFSDPKGLEGLAHFLEHMLFYASEK 180
            KR+YR I+L N+L++LLISDPETDKCAASMNVCVG+FSDP+GLEGLAHFLEHMLFYASEK
Sbjct: 21   KRDYRRIVLQNNLEILLISDPETDKCAASMNVCVGAFSDPEGLEGLAHFLEHMLFYASEK 80

Query: 181  YPLEDSYSKYITEHGGSTNAFTSSEQTNFYFDVNADCFEEALDRFAQFFIKPLMSSDATT 360
            YP+EDSYSKYITE+GGSTNAFTSSE TN+YF+VNAD FEEALDRFAQFFIKPLMS+DATT
Sbjct: 81   YPVEDSYSKYITENGGSTNAFTSSEDTNYYFEVNADGFEEALDRFAQFFIKPLMSADATT 140

Query: 361  REIKAVDSENQKNLLSDAWRMNQLQKHLSAEDHPYHKFSTGNWDTLEVRPKSKGVDTRQE 540
            REIKAVDSE+QKNLLSD WRMNQLQKHLSAE+HPYHKFSTG+WDTLEVRPK +G+DTRQE
Sbjct: 141  REIKAVDSEHQKNLLSDPWRMNQLQKHLSAENHPYHKFSTGSWDTLEVRPKERGIDTRQE 200

Query: 541  LLKFYEQNYSSNLMCLVVYGKESLDKIGSQVVDKFQDISNRDRSSLHFPGQPCMPEHLQI 720
            LLKFY +NYS+NLM LVVY K+SLDK+   V  KFQDI N DR+ +HF GQPC+ EHLQI
Sbjct: 201  LLKFYSENYSANLMHLVVYSKDSLDKVEQLVRGKFQDIRNIDRNQIHFTGQPCIMEHLQI 260

Query: 721  LVKTVPIKQGHKLRFTWPVTPGILHYKEGPSRYLGHLIGHEGEGSLFYALKKLGWATSLS 900
            LV+ VPIKQGHKL+  WP+TPGI HYKEGP RYLGHLIGHEGEGSLFY LKKLGWATSLS
Sbjct: 261  LVRAVPIKQGHKLKIIWPITPGIHHYKEGPCRYLGHLIGHEGEGSLFYVLKKLGWATSLS 320

Query: 901  AGESDETREFSFFKVNIDLTDAGHEHFEDIVALLFKYIRVLQQSGVCKWIFDELSAICET 1080
            AGESD T EFSFFKV IDLTDAG +HFEDI+ LLFKYI +LQQ+G  KWIF+ELSAICET
Sbjct: 321  AGESDWTNEFSFFKVAIDLTDAGQDHFEDIMGLLFKYIHLLQQAGASKWIFEELSAICET 380

Query: 1081 AFHYQDKIRPMDYVVNVASNMQLYPTRDWLVGSSLPSTFNPDIIQSILNELSPDSVRIFW 1260
            AFHYQDKIRP DYVVNVA NMQ YP  DWLV SSLPS FNP IIQS LNEL+PD+VRIFW
Sbjct: 381  AFHYQDKIRPSDYVVNVAMNMQHYPPEDWLVASSLPSKFNPSIIQSFLNELNPDNVRIFW 440

Query: 1261 SSMKFEGQTDKTEPWYGTAYSVEKMTRSLIEKWIERAPEEHLHLPTQNVFIPTDLTLKTA 1440
             S KFEG T  TEPWYGTAYS+EK+    I++W+E AP E LHLP  NVFIPTDL+LK  
Sbjct: 441  ESTKFEGNTSMTEPWYGTAYSIEKVGGDSIKQWMEHAPSEELHLPAPNVFIPTDLSLKPV 500

Query: 1441 PEKANLPLLLRKSPYSRLWYKSDSTFFTPKAYVKIDFNCPFAGNSPEAEVLTDIFTRLVT 1620
             EK  +P+LLRKSPYSRLWYK D+ F +PKAYV IDF+CP+ G+SPEAEVLT+IFTRL+ 
Sbjct: 501  FEKTKVPILLRKSPYSRLWYKPDTAFSSPKAYVMIDFSCPYCGHSPEAEVLTEIFTRLLM 560

Query: 1621 DYLNEYAYYAQVAGLYYAVNHTDNGFQVTIVGYNHKLKILLDTVIEKIANFEVKPDRFSV 1800
            DYLNEYAY AQVAGLYY ++ T++GFQ+T+ GYN KL++LL+ VIEK+A FEVKPDRFSV
Sbjct: 561  DYLNEYAYNAQVAGLYYDISKTNSGFQLTLFGYNDKLRVLLEAVIEKVAKFEVKPDRFSV 620

Query: 1801 IKELITKDYKNFKFQQPYQQAMYYCSLILQDQTWPWSDQLEALPHLTVDHLAKFYPLMIS 1980
            +KEL+TK Y+NFKFQQPYQQ MYYCSL+L+D  WPW+++L+ LPHL VD L KFYPL+++
Sbjct: 621  VKELVTKQYQNFKFQQPYQQVMYYCSLLLKDNIWPWNEELDVLPHLKVDDLVKFYPLLMA 680

Query: 1981 KTFIECYVAGNIEPKEAELIIQHVETVFYKGQDPKSKALFPSQHLTNRVVKLDRGTNYFY 2160
            ++F+ECYVAGN+E  EAE +IQ +E VF+KG    SK LF SQHLTNRVV L+RG NY Y
Sbjct: 681  RSFMECYVAGNVEQAEAESMIQLIEDVFFKGPQSISKPLFASQHLTNRVVNLERGVNYVY 740

Query: 2161 TAEGLNSSDENSAIVHYIQVHQDDYMLNVKLQLFALVAKQPAFHQLRSVEQLGYITVLMQ 2340
             AEGLN SDENSA+VHYIQVHQDD+MLNVKLQLFAL+AKQPAFHQLRSVEQLGYITVLMQ
Sbjct: 741  AAEGLNPSDENSALVHYIQVHQDDFMLNVKLQLFALIAKQPAFHQLRSVEQLGYITVLMQ 800

Query: 2341 RNDSGVRGVQFIIQSTAKGPRHIDLRVESFLKMFESKLQEMSNEEFKSNVNALIDMKLEK 2520
            R+DSGV GVQFI+QSTAK P++ID RVE F+KMFESKL EM+++EFK+NVNALIDMKLEK
Sbjct: 801  RSDSGVHGVQFIVQSTAKDPKYIDTRVELFMKMFESKLYEMTSDEFKNNVNALIDMKLEK 860

Query: 2521 HKN 2529
            HKN
Sbjct: 861  HKN 863



 Score =  138 bits (348), Expect = 8e-30
 Identities = 64/89 (71%), Positives = 79/89 (88%)
 Frame = +2

Query: 2621 DRREREVAALKQVTHKDLIDFFNEHIKVGAPQKKSLSVRVYGSSHSSEYEEDKKESLEPN 2800
            DRR+RE+ ALKQ+T K+L DFF+E+IKVG P+KK+LSVRVYGSSHSS+++  K E +EPN
Sbjct: 883  DRRDREIVALKQLTQKELTDFFDEYIKVGVPRKKALSVRVYGSSHSSQFQAHKNEQMEPN 942

Query: 2801 YVQIEDIFSFRRSRPLYSSFKGGVGHMKL 2887
             VQIE+IFSFRRSRPLYSSFKGG GH++L
Sbjct: 943  AVQIEEIFSFRRSRPLYSSFKGGFGHVRL 971


>ref|XP_002308028.1| predicted protein [Populus trichocarpa] gi|222854004|gb|EEE91551.1|
            predicted protein [Populus trichocarpa]
          Length = 949

 Score = 1330 bits (3443), Expect = 0.0
 Identities = 637/843 (75%), Positives = 722/843 (85%)
 Frame = +1

Query: 1    KREYRNIILHNSLQVLLISDPETDKCAASMNVCVGSFSDPKGLEGLAHFLEHMLFYASEK 180
            KREY+ I+L N+LQ           CAASMNV VG FSDP GLEGLAHFLEHMLFYASEK
Sbjct: 10   KREYKRIVLPNALQ-----------CAASMNVSVGCFSDPDGLEGLAHFLEHMLFYASEK 58

Query: 181  YPLEDSYSKYITEHGGSTNAFTSSEQTNFYFDVNADCFEEALDRFAQFFIKPLMSSDATT 360
            YPLEDSYSKYI EHGGSTNA+T+S+ TN++FDVN+DCFE+ALDRFAQFFIKPLMS+DAT 
Sbjct: 59   YPLEDSYSKYIIEHGGSTNAYTTSDHTNYHFDVNSDCFEDALDRFAQFFIKPLMSADATV 118

Query: 361  REIKAVDSENQKNLLSDAWRMNQLQKHLSAEDHPYHKFSTGNWDTLEVRPKSKGVDTRQE 540
            REIKAVDSENQKNLLSD WR+NQLQKHLS E HPYHKFSTGNWDTLEV+PK KG+DTR E
Sbjct: 119  REIKAVDSENQKNLLSDGWRINQLQKHLSEEGHPYHKFSTGNWDTLEVQPKEKGLDTRLE 178

Query: 541  LLKFYEQNYSSNLMCLVVYGKESLDKIGSQVVDKFQDISNRDRSSLHFPGQPCMPEHLQI 720
            L+K YE+NYS+NLM LV+Y KESLDKI S V +KFQ+I N DRS   FPGQPC  EHLQI
Sbjct: 179  LIKLYEENYSANLMNLVIYAKESLDKIQSLVEEKFQEIRNNDRSCFSFPGQPCSSEHLQI 238

Query: 721  LVKTVPIKQGHKLRFTWPVTPGILHYKEGPSRYLGHLIGHEGEGSLFYALKKLGWATSLS 900
            LV+TVPIKQGHKLR  WP+TPGILHYKEGP RYLGHLIGHEGEGSLFY LK LGWAT LS
Sbjct: 239  LVRTVPIKQGHKLRIVWPITPGILHYKEGPCRYLGHLIGHEGEGSLFYVLKTLGWATDLS 298

Query: 901  AGESDETREFSFFKVNIDLTDAGHEHFEDIVALLFKYIRVLQQSGVCKWIFDELSAICET 1080
            AGE D T EF+FF   I+LTDAGHEH +D+V LLFKYI +LQQSGVCKWIFDEL+AICET
Sbjct: 299  AGEVDGTTEFAFFTAVINLTDAGHEHMQDVVGLLFKYIHLLQQSGVCKWIFDELAAICET 358

Query: 1081 AFHYQDKIRPMDYVVNVASNMQLYPTRDWLVGSSLPSTFNPDIIQSILNELSPDSVRIFW 1260
            +FHYQDK  P+ YVV +ASNMQLYP +DWLVGSSLPS F+P IIQ++LN+LSPD+VRIFW
Sbjct: 359  SFHYQDKTPPISYVVRIASNMQLYPQKDWLVGSSLPSNFSPSIIQTVLNQLSPDNVRIFW 418

Query: 1261 SSMKFEGQTDKTEPWYGTAYSVEKMTRSLIEKWIERAPEEHLHLPTQNVFIPTDLTLKTA 1440
             S KFEGQT  TEPWY TAYSVEK+T S+I++W+  AP E LHLP  NVFIPTDL+LK A
Sbjct: 419  ESKKFEGQTAMTEPWYKTAYSVEKITGSMIQEWMLFAPNEDLHLPAPNVFIPTDLSLKDA 478

Query: 1441 PEKANLPLLLRKSPYSRLWYKSDSTFFTPKAYVKIDFNCPFAGNSPEAEVLTDIFTRLVT 1620
             EK   P+LLRKS  S LWYK D+ F TPKAYVKIDFNCPFA +SPE EVLTDIF RL+ 
Sbjct: 479  QEKVKFPVLLRKSSSSSLWYKPDTMFSTPKAYVKIDFNCPFASSSPETEVLTDIFARLLM 538

Query: 1621 DYLNEYAYYAQVAGLYYAVNHTDNGFQVTIVGYNHKLKILLDTVIEKIANFEVKPDRFSV 1800
            D LN+YAYYAQVAGLYY +++TD+GFQVT+VGYNHKL+ILL+TVIEKI+NF+VKPDRFSV
Sbjct: 539  DDLNDYAYYAQVAGLYYGISNTDSGFQVTVVGYNHKLRILLETVIEKISNFKVKPDRFSV 598

Query: 1801 IKELITKDYKNFKFQQPYQQAMYYCSLILQDQTWPWSDQLEALPHLTVDHLAKFYPLMIS 1980
            IKE++TK+Y N KFQQPYQQAMYYCSL+LQDQTWPW +QLE LPHL  + LAKF PLM+S
Sbjct: 599  IKEMVTKEYGNLKFQQPYQQAMYYCSLLLQDQTWPWMEQLEILPHLQAEDLAKFIPLMLS 658

Query: 1981 KTFIECYVAGNIEPKEAELIIQHVETVFYKGQDPKSKALFPSQHLTNRVVKLDRGTNYFY 2160
            + F+ECY+AGNIE  EAE +I H+E VF +G DP  + LFPSQHLT+RV+KL+RG NY Y
Sbjct: 659  RAFLECYIAGNIERSEAESMILHIEDVFNEGPDPICQPLFPSQHLTSRVIKLERGINYLY 718

Query: 2161 TAEGLNSSDENSAIVHYIQVHQDDYMLNVKLQLFALVAKQPAFHQLRSVEQLGYITVLMQ 2340
              EGLN  DENSA+VHYIQ+H+DD+  NVKLQL AL+AKQPAFHQLRSVEQLGYITVLMQ
Sbjct: 719  PIEGLNPDDENSALVHYIQIHRDDFTWNVKLQLLALIAKQPAFHQLRSVEQLGYITVLMQ 778

Query: 2341 RNDSGVRGVQFIIQSTAKGPRHIDLRVESFLKMFESKLQEMSNEEFKSNVNALIDMKLEK 2520
            RNDSG+RG+QFIIQST KGP  IDLRVE+FLKMFE+KL  M+N+EFKSNVNALIDMKLEK
Sbjct: 779  RNDSGIRGLQFIIQSTVKGPGQIDLRVEAFLKMFETKLYGMTNDEFKSNVNALIDMKLEK 838

Query: 2521 HKN 2529
            HKN
Sbjct: 839  HKN 841



 Score =  140 bits (352), Expect = 3e-30
 Identities = 67/89 (75%), Positives = 76/89 (85%)
 Frame = +2

Query: 2621 DRREREVAALKQVTHKDLIDFFNEHIKVGAPQKKSLSVRVYGSSHSSEYEEDKKESLEPN 2800
            DRRE EVAALKQ+T +DLIDFF+EH+KVGAP+K++LSVRVYG  HS EY  DK + L PN
Sbjct: 861  DRRECEVAALKQLTQQDLIDFFDEHVKVGAPRKRTLSVRVYGKLHSCEYPSDKSQQLPPN 920

Query: 2801 YVQIEDIFSFRRSRPLYSSFKGGVGHMKL 2887
             VQIEDIFSFRRS+PLY SFKGG GHMKL
Sbjct: 921  AVQIEDIFSFRRSQPLYGSFKGGFGHMKL 949


>ref|XP_002283970.1| PREDICTED: zinc-metallopeptidase, peroxisomal [Vitis vinifera]
            gi|297739662|emb|CBI29844.3| unnamed protein product
            [Vitis vinifera]
          Length = 965

 Score = 1322 bits (3421), Expect = 0.0
 Identities = 633/842 (75%), Positives = 724/842 (85%)
 Frame = +1

Query: 4    REYRNIILHNSLQVLLISDPETDKCAASMNVCVGSFSDPKGLEGLAHFLEHMLFYASEKY 183
            REYR I+L NSL+ LLISDP+TDK AASM+V VGSFSDP+G  GLAHFLEHMLFYASEKY
Sbjct: 16   REYRRIVLRNSLEALLISDPDTDKAAASMSVSVGSFSDPEGFPGLAHFLEHMLFYASEKY 75

Query: 184  PLEDSYSKYITEHGGSTNAFTSSEQTNFYFDVNADCFEEALDRFAQFFIKPLMSSDATTR 363
            PLEDSYSKYITEHGGSTNAFT+SE TN++FDVN DCFEEALDRFAQFF+KPLMS+DATTR
Sbjct: 76   PLEDSYSKYITEHGGSTNAFTASEHTNYFFDVNTDCFEEALDRFAQFFVKPLMSADATTR 135

Query: 364  EIKAVDSENQKNLLSDAWRMNQLQKHLSAEDHPYHKFSTGNWDTLEVRPKSKGVDTRQEL 543
            EIKAVDSEN+KNLLSDAWRM+QLQKH+SAE HPYHKFSTGN DTLEV+PK KG+DTR EL
Sbjct: 136  EIKAVDSENKKNLLSDAWRMDQLQKHVSAEGHPYHKFSTGNRDTLEVKPKEKGLDTRHEL 195

Query: 544  LKFYEQNYSSNLMCLVVYGKESLDKIGSQVVDKFQDISNRDRSSLHFPGQPCMPEHLQIL 723
            +KFYE++YS+NLM LVVY KESLDKI S V  KFQ+I N+DR + H  GQPC  EHLQIL
Sbjct: 196  IKFYEEHYSANLMHLVVYTKESLDKIQSLVEHKFQEIQNKDRINFHICGQPCTSEHLQIL 255

Query: 724  VKTVPIKQGHKLRFTWPVTPGILHYKEGPSRYLGHLIGHEGEGSLFYALKKLGWATSLSA 903
            VKTVPIKQGHKL   WP+TP I +YKEGP RYLGHLIGHEG+GSLFY LK LGWATSLSA
Sbjct: 256  VKTVPIKQGHKLIVVWPITPSIHNYKEGPCRYLGHLIGHEGKGSLFYILKTLGWATSLSA 315

Query: 904  GESDETREFSFFKVNIDLTDAGHEHFEDIVALLFKYIRVLQQSGVCKWIFDELSAICETA 1083
             E D T EFSFF+V IDLTDAGHEH +DIV LLFKYI +LQQ+GVCKWIFDELSAICET 
Sbjct: 316  WEEDWTCEFSFFEVVIDLTDAGHEHMQDIVGLLFKYISLLQQTGVCKWIFDELSAICETM 375

Query: 1084 FHYQDKIRPMDYVVNVASNMQLYPTRDWLVGSSLPSTFNPDIIQSILNELSPDSVRIFWS 1263
            FHYQDKI  +DYVVNV+SNM+LYP +DWLVGSSLPS F+PD+IQ +L+EL+P++VRIFW 
Sbjct: 376  FHYQDKIPSIDYVVNVSSNMELYPPKDWLVGSSLPSKFSPDVIQKVLDELAPNNVRIFWE 435

Query: 1264 SMKFEGQTDKTEPWYGTAYSVEKMTRSLIEKWIERAPEEHLHLPTQNVFIPTDLTLKTAP 1443
            S  FEG TD  EPWYGTA+S+EK+T S+I++W+  AP EHLHLP  N FIPTDL+LK   
Sbjct: 436  SKNFEGHTDMVEPWYGTAFSIEKITVSMIQQWMLAAPTEHLHLPDPNDFIPTDLSLKNVQ 495

Query: 1444 EKANLPLLLRKSPYSRLWYKSDSTFFTPKAYVKIDFNCPFAGNSPEAEVLTDIFTRLVTD 1623
            EKA  P+LLRKS YS LWYK D+ F TPKAYVKIDFNCPFA +SPEA+VLTDIFTRL+ D
Sbjct: 496  EKAKFPVLLRKSSYSTLWYKPDTMFSTPKAYVKIDFNCPFASSSPEADVLTDIFTRLLMD 555

Query: 1624 YLNEYAYYAQVAGLYYAVNHTDNGFQVTIVGYNHKLKILLDTVIEKIANFEVKPDRFSVI 1803
            YLNE AYYA+VAGLYY +++TD+GFQV + GYNHKL+ILL+TV++KIANF+VKPDRF VI
Sbjct: 556  YLNEDAYYAEVAGLYYCLSNTDSGFQVAMAGYNHKLRILLETVVKKIANFKVKPDRFLVI 615

Query: 1804 KELITKDYKNFKFQQPYQQAMYYCSLILQDQTWPWSDQLEALPHLTVDHLAKFYPLMISK 1983
            KEL+TK Y+N KFQQPYQQAM Y SLIL D TWPW D LE +PHL  D LAKF P+++S+
Sbjct: 616  KELVTKGYQNVKFQQPYQQAMCYRSLILHDNTWPWMDGLEVIPHLEADDLAKFVPMLLSR 675

Query: 1984 TFIECYVAGNIEPKEAELIIQHVETVFYKGQDPKSKALFPSQHLTNRVVKLDRGTNYFYT 2163
             F+ECY+AGNIEPKEAE +I H+E +FY G  P  + LFPSQ+LTNRV+KLDRG +YFY 
Sbjct: 676  AFLECYIAGNIEPKEAEAMIHHIEDIFYSGPRPICQPLFPSQYLTNRVIKLDRGMSYFYP 735

Query: 2164 AEGLNSSDENSAIVHYIQVHQDDYMLNVKLQLFALVAKQPAFHQLRSVEQLGYITVLMQR 2343
            AEGLN SDENSA+VHYIQVH+DD++ NVKLQLFAL+AKQ AFHQLRSVEQLGYIT LM R
Sbjct: 736  AEGLNPSDENSALVHYIQVHRDDFLPNVKLQLFALIAKQQAFHQLRSVEQLGYITELMLR 795

Query: 2344 NDSGVRGVQFIIQSTAKGPRHIDLRVESFLKMFESKLQEMSNEEFKSNVNALIDMKLEKH 2523
            NDSG+ GVQF+IQST KGP HID R+E FLKMFE KL  MS +EFKSNVN L+DMKLEK+
Sbjct: 796  NDSGIHGVQFMIQSTVKGPGHIDSRIEEFLKMFEFKLYAMSEDEFKSNVNTLVDMKLEKY 855

Query: 2524 KN 2529
            KN
Sbjct: 856  KN 857



 Score =  134 bits (336), Expect = 2e-28
 Identities = 63/89 (70%), Positives = 78/89 (87%)
 Frame = +2

Query: 2621 DRREREVAALKQVTHKDLIDFFNEHIKVGAPQKKSLSVRVYGSSHSSEYEEDKKESLEPN 2800
            DR E EVAALK++T K+LIDFFNEHIKVGAPQKK+LSVRVYG  H+SEY E++KE+ +P 
Sbjct: 877  DRTEAEVAALKKLTQKELIDFFNEHIKVGAPQKKTLSVRVYGCLHTSEYAEEQKEANQPI 936

Query: 2801 YVQIEDIFSFRRSRPLYSSFKGGVGHMKL 2887
             V+I+DIF FR+S+PLY SFKGG+GH+KL
Sbjct: 937  QVKIDDIFKFRKSQPLYGSFKGGLGHVKL 965