BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00000336
         (1756 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CAA73139.1| isocitrate dehydrogenase (NADP+) [Apium graveolens]   457   e-126
dbj|BAA34112.1| NADP specific isocitrate dehydrogenase [Daucus c...   454   e-125
ref|XP_002305928.1| predicted protein [Populus trichocarpa] gi|2...   441   e-121
dbj|BAC77063.1| NADP-specific isocitrate dehydrogenase [Lupinus ...   441   e-121
gb|ADU20197.1| NADP-dependent isocitric acid dehydrogenase [Pyru...   440   e-121

>emb|CAA73139.1| isocitrate dehydrogenase (NADP+) [Apium graveolens]
          Length = 412

 Score =  457 bits (1175), Expect = e-126
 Identities = 223/229 (97%), Positives = 228/229 (99%)
 Frame = +3

Query: 735  MYNTDESIRAFAEASMTTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEANWKSKYEA 914
            MYNTDESIR+FAEASMTTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEA+WKSKYEA
Sbjct: 184  MYNTDESIRSFAEASMTTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEASWKSKYEA 243

Query: 915  AGIWYEHRLIDDMVAYALKSDGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGK 1094
            AGIWYEHRLIDDMVAYALKSDGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGK
Sbjct: 244  AGIWYEHRLIDDMVAYALKSDGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGK 303

Query: 1095 TIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWSRGLAHRAKLDDNAALLNFAEKLEA 1274
            TIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWSRGLAHRAKLDDNAALL+FAEKLEA
Sbjct: 304  TIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWSRGLAHRAKLDDNAALLSFAEKLEA 363

Query: 1275 ACVNTVESGKMTKDLALILHGSKLSREHYLNTEEFIDAVADDLKARLAN 1421
            ACV TVE+G+MTKDLALILHGSKLSREHYLNTEEFIDAVADDLKARLAN
Sbjct: 364  ACVGTVEAGRMTKDLALILHGSKLSREHYLNTEEFIDAVADDLKARLAN 412



 Score =  313 bits (801), Expect = 1e-82
 Identities = 149/154 (96%), Positives = 153/154 (99%)
 Frame = +1

Query: 127 MAFEKIKVANPIVEMDGDEMTRIFWKSIKDKLIFPFVELDIKYFDLGLPHRDETDDKVTV 306
           MAF+KIKVANPIVEMDGDEMTR+FWKSIKDKLIFPFVELDIKYFDLGLPHRD TDDKVTV
Sbjct: 1   MAFQKIKVANPIVEMDGDEMTRVFWKSIKDKLIFPFVELDIKYFDLGLPHRDATDDKVTV 60

Query: 307 ESAEATLKYNVAIKCATITPDEARVKEFGLKQMWKSPNGTIRNILNGTVFREPIICKNIP 486
           ESAEATLKYNVAIKCATITPDEARV+EFGLKQMWKSPNGTIRNILNGTVFREPIICKNIP
Sbjct: 61  ESAEATLKYNVAIKCATITPDEARVEEFGLKQMWKSPNGTIRNILNGTVFREPIICKNIP 120

Query: 487 KLIPGWTKPICIGRHAFGDQYRATDAVIQGPGKL 588
           KLIPGWTKPICIGRHAFGDQYRATDAVI+GPGKL
Sbjct: 121 KLIPGWTKPICIGRHAFGDQYRATDAVIKGPGKL 154


>dbj|BAA34112.1| NADP specific isocitrate dehydrogenase [Daucus carota]
          Length = 412

 Score =  454 bits (1167), Expect = e-125
 Identities = 222/229 (96%), Positives = 226/229 (98%)
 Frame = +3

Query: 735  MYNTDESIRAFAEASMTTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEANWKSKYEA 914
            MYNTDESIR+FAEASM TAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEA+WKSKY+A
Sbjct: 184  MYNTDESIRSFAEASMATAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEASWKSKYDA 243

Query: 915  AGIWYEHRLIDDMVAYALKSDGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGK 1094
            AGIWYEHRLIDDMVAYALKSDGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGK
Sbjct: 244  AGIWYEHRLIDDMVAYALKSDGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGK 303

Query: 1095 TIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWSRGLAHRAKLDDNAALLNFAEKLEA 1274
            TIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWSRGLAHRAKLDDNAALL+FAEKLEA
Sbjct: 304  TIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWSRGLAHRAKLDDNAALLSFAEKLEA 363

Query: 1275 ACVNTVESGKMTKDLALILHGSKLSREHYLNTEEFIDAVADDLKARLAN 1421
            ACV TVESGKMTKDLALILHGSKLSREHYLNTEEFIDAVA DLKARLAN
Sbjct: 364  ACVGTVESGKMTKDLALILHGSKLSREHYLNTEEFIDAVASDLKARLAN 412



 Score =  316 bits (809), Expect = 1e-83
 Identities = 151/154 (98%), Positives = 153/154 (99%)
 Frame = +1

Query: 127 MAFEKIKVANPIVEMDGDEMTRIFWKSIKDKLIFPFVELDIKYFDLGLPHRDETDDKVTV 306
           MAF+KIKVANPIVEMDGDEMTR+FWKSIKDKLIFPFVELDIKYFDLGLPHRD TDDKVTV
Sbjct: 1   MAFQKIKVANPIVEMDGDEMTRVFWKSIKDKLIFPFVELDIKYFDLGLPHRDATDDKVTV 60

Query: 307 ESAEATLKYNVAIKCATITPDEARVKEFGLKQMWKSPNGTIRNILNGTVFREPIICKNIP 486
           ESAEATLKYNVAIKCATITPDEARVKEFGLKQMWKSPNGTIRNILNGTVFREPIICKNIP
Sbjct: 61  ESAEATLKYNVAIKCATITPDEARVKEFGLKQMWKSPNGTIRNILNGTVFREPIICKNIP 120

Query: 487 KLIPGWTKPICIGRHAFGDQYRATDAVIQGPGKL 588
           KLIPGWTKPICIGRHAFGDQYRATDAVIQGPGKL
Sbjct: 121 KLIPGWTKPICIGRHAFGDQYRATDAVIQGPGKL 154


>ref|XP_002305928.1| predicted protein [Populus trichocarpa] gi|222848892|gb|EEE86439.1|
            predicted protein [Populus trichocarpa]
          Length = 414

 Score =  441 bits (1135), Expect = e-121
 Identities = 212/228 (92%), Positives = 223/228 (97%)
 Frame = +3

Query: 735  MYNTDESIRAFAEASMTTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEANWKSKYEA 914
            MYNTDESIRAFAEASM TAY+KKWPLYLSTKNTILKKYDGRFKDIFQEVYEANWKSKYEA
Sbjct: 184  MYNTDESIRAFAEASMNTAYQKKWPLYLSTKNTILKKYDGRFKDIFQEVYEANWKSKYEA 243

Query: 915  AGIWYEHRLIDDMVAYALKSDGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGK 1094
            AGIWYEHRLIDDMVAYALKS+GGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGK
Sbjct: 244  AGIWYEHRLIDDMVAYALKSEGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGK 303

Query: 1095 TIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWSRGLAHRAKLDDNAALLNFAEKLEA 1274
            TIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWSRGLAHRAKLDDNA LL+F EKLEA
Sbjct: 304  TIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWSRGLAHRAKLDDNARLLDFTEKLEA 363

Query: 1275 ACVNTVESGKMTKDLALILHGSKLSREHYLNTEEFIDAVADDLKARLA 1418
            AC+  VESGKMTKDLAL++HGSK+SR+HYLNTEEFIDAVA++LKARL+
Sbjct: 364  ACIGAVESGKMTKDLALLIHGSKVSRDHYLNTEEFIDAVAEELKARLS 411



 Score =  304 bits (779), Expect = 4e-80
 Identities = 145/154 (94%), Positives = 150/154 (97%)
 Frame = +1

Query: 127 MAFEKIKVANPIVEMDGDEMTRIFWKSIKDKLIFPFVELDIKYFDLGLPHRDETDDKVTV 306
           MA+EKIKVANPIVEMDGDEMTRIFW+SIKDKLIFPFVELDIKYFDLGLPHRD TDDKVTV
Sbjct: 1   MAYEKIKVANPIVEMDGDEMTRIFWQSIKDKLIFPFVELDIKYFDLGLPHRDATDDKVTV 60

Query: 307 ESAEATLKYNVAIKCATITPDEARVKEFGLKQMWKSPNGTIRNILNGTVFREPIICKNIP 486
           ESAEA LKYNVAIKCATITPDEARVKEF LKQMWKSPNGTIRNILNGTVFREPIICKNIP
Sbjct: 61  ESAEAALKYNVAIKCATITPDEARVKEFNLKQMWKSPNGTIRNILNGTVFREPIICKNIP 120

Query: 487 KLIPGWTKPICIGRHAFGDQYRATDAVIQGPGKL 588
           +L+PGWTKPICIGRHAFGDQYRATDAVI+G GKL
Sbjct: 121 RLVPGWTKPICIGRHAFGDQYRATDAVIKGAGKL 154


>dbj|BAC77063.1| NADP-specific isocitrate dehydrogenase [Lupinus albus]
          Length = 412

 Score =  441 bits (1134), Expect = e-121
 Identities = 213/228 (93%), Positives = 224/228 (98%)
 Frame = +3

Query: 735  MYNTDESIRAFAEASMTTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEANWKSKYEA 914
            MYNTDESIRAFAEASMTTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEA+WKSK+EA
Sbjct: 184  MYNTDESIRAFAEASMTTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEASWKSKFEA 243

Query: 915  AGIWYEHRLIDDMVAYALKSDGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGK 1094
            AGIWYEHRLIDDMVAYA+KSDGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGK
Sbjct: 244  AGIWYEHRLIDDMVAYAVKSDGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGK 303

Query: 1095 TIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWSRGLAHRAKLDDNAALLNFAEKLEA 1274
            TIE+EAAHGTVTRH+RVHQKGGETSTNSIASIFAWSRGLAHRAKLDDNA LL+F +KLEA
Sbjct: 304  TIESEAAHGTVTRHFRVHQKGGETSTNSIASIFAWSRGLAHRAKLDDNAKLLDFTQKLEA 363

Query: 1275 ACVNTVESGKMTKDLALILHGSKLSREHYLNTEEFIDAVADDLKARLA 1418
            AC+  VESGKMTKDLALILHGSKLSREHYLNTEEFIDAVA++LKARL+
Sbjct: 364  ACIGAVESGKMTKDLALILHGSKLSREHYLNTEEFIDAVANELKARLS 411



 Score =  296 bits (758), Expect = 1e-77
 Identities = 138/154 (89%), Positives = 149/154 (96%)
 Frame = +1

Query: 127 MAFEKIKVANPIVEMDGDEMTRIFWKSIKDKLIFPFVELDIKYFDLGLPHRDETDDKVTV 306
           MAF+KI VANPIVEMDGDEMTR+ WKSIKDKLIFPF+ELDIKY+DLGLP+RDETDDKVTV
Sbjct: 1   MAFQKINVANPIVEMDGDEMTRVIWKSIKDKLIFPFLELDIKYYDLGLPYRDETDDKVTV 60

Query: 307 ESAEATLKYNVAIKCATITPDEARVKEFGLKQMWKSPNGTIRNILNGTVFREPIICKNIP 486
           ESAEATLKYNVAIKCATITPDE RVKEFGLKQMW+SPNGTIRNILNGTVFREPI+CKNIP
Sbjct: 61  ESAEATLKYNVAIKCATITPDEGRVKEFGLKQMWRSPNGTIRNILNGTVFREPILCKNIP 120

Query: 487 KLIPGWTKPICIGRHAFGDQYRATDAVIQGPGKL 588
           +L+PGW KPICIGRHAFGDQYRATD+VI+G GKL
Sbjct: 121 RLLPGWNKPICIGRHAFGDQYRATDSVIKGAGKL 154


>gb|ADU20197.1| NADP-dependent isocitric acid dehydrogenase [Pyrus pyrifolia]
          Length = 412

 Score =  440 bits (1131), Expect = e-121
 Identities = 213/228 (93%), Positives = 221/228 (96%)
 Frame = +3

Query: 735  MYNTDESIRAFAEASMTTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEANWKSKYEA 914
            MYNTDESIRAFAEASMTTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEANWKSK+EA
Sbjct: 184  MYNTDESIRAFAEASMTTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEANWKSKFEA 243

Query: 915  AGIWYEHRLIDDMVAYALKSDGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGK 1094
            AGIWYEHRLIDDMVAYALKSDGGYVWACKNYDGDVQSD LAQGFGSLGLMTSVLVCPDGK
Sbjct: 244  AGIWYEHRLIDDMVAYALKSDGGYVWACKNYDGDVQSDLLAQGFGSLGLMTSVLVCPDGK 303

Query: 1095 TIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWSRGLAHRAKLDDNAALLNFAEKLEA 1274
            TIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAW+RGLAHRAKLDDNA LL F +KLE 
Sbjct: 304  TIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWTRGLAHRAKLDDNARLLEFTQKLEE 363

Query: 1275 ACVNTVESGKMTKDLALILHGSKLSREHYLNTEEFIDAVADDLKARLA 1418
            AC+ TVESGKMTKDLALILHGSKL+R HYLNTEEFIDAVA++LKA+LA
Sbjct: 364  ACIGTVESGKMTKDLALILHGSKLARNHYLNTEEFIDAVANELKAKLA 411



 Score =  302 bits (774), Expect = 2e-79
 Identities = 142/154 (92%), Positives = 150/154 (97%)
 Frame = +1

Query: 127 MAFEKIKVANPIVEMDGDEMTRIFWKSIKDKLIFPFVELDIKYFDLGLPHRDETDDKVTV 306
           MAF+KIKVANPIVEMDGDEMTR+FWKSIKDKLI PFVELDIKYFDLGLPHRD TDDKVTV
Sbjct: 1   MAFQKIKVANPIVEMDGDEMTRVFWKSIKDKLILPFVELDIKYFDLGLPHRDATDDKVTV 60

Query: 307 ESAEATLKYNVAIKCATITPDEARVKEFGLKQMWKSPNGTIRNILNGTVFREPIICKNIP 486
           ESAEATLKYNVAIKCATITPDEAR+KEF LK MW+SPNGTIRNILNGTVFREPI+CKNIP
Sbjct: 61  ESAEATLKYNVAIKCATITPDEARMKEFSLKSMWRSPNGTIRNILNGTVFREPILCKNIP 120

Query: 487 KLIPGWTKPICIGRHAFGDQYRATDAVIQGPGKL 588
           +LIPGWT+PICIGRHAFGDQYRATDAVI+GPGKL
Sbjct: 121 RLIPGWTRPICIGRHAFGDQYRATDAVIKGPGKL 154