BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00000276
         (2615 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002282994.1| PREDICTED: calcium-dependent protein kinase ...   959   0.0  
ref|XP_002520611.1| calcium-dependent protein kinase, putative [...   955   0.0  
emb|CAN78387.1| hypothetical protein VITISV_017368 [Vitis vinifera]   954   0.0  
gb|AAL68971.1| phloem calmodulin-like-domain protein kinase PCPK...   951   0.0  
ref|XP_004144896.1| PREDICTED: calcium-dependent protein kinase ...   949   0.0  

>ref|XP_002282994.1| PREDICTED: calcium-dependent protein kinase 4 [Vitis vinifera]
          Length = 561

 Score =  959 bits (2478), Expect = 0.0
 Identities = 482/569 (84%), Positives = 515/569 (90%), Gaps = 1/569 (0%)
 Frame = -2

Query: 1912 MGNSCSGSFGVKEYQGCTQPEDRSKCXXXXXXXXXXXXXXSEYSPTTLTAQQLVAQEFVK 1733
            MGN+C GSF  K +QG  QPE++S                 +YSP++L +QQLV+QEF K
Sbjct: 1    MGNTCRGSFRGKYFQGYGQPEEQSTSKRNSDRSNS------DYSPSSLNSQQLVSQEFAK 54

Query: 1732 DDQTIPKKDHTLPIVSP-KKDNTMSRSGNNYSCYVLGHATPNIRDVYTIGRKLGQGQFGI 1556
            ++   PKK+  + I+SP  KD TM R  +N S YVLGH T NIRD+Y++GRKLGQGQFG 
Sbjct: 55   EN---PKKETRVSILSPTNKDGTMRRGVDNQSYYVLGHKTANIRDLYSLGRKLGQGQFGT 111

Query: 1555 TYLCTEIATGIEYACKSISKRKLISREDVEDVRREIQIMHHLAGHKNIVTIKGAYEDHLY 1376
            TYLCT++ATGIEYACKSISKRKLISREDVEDVRREIQIMHHLAGHKNIVTIKGAYED LY
Sbjct: 112  TYLCTDMATGIEYACKSISKRKLISREDVEDVRREIQIMHHLAGHKNIVTIKGAYEDPLY 171

Query: 1375 VHIVMELCNGGELFDRIIERGHYSERKAAELIKIIVGVVESCHSLGVMHRDLKPENFLLV 1196
            VHIVMELC GGELFDRII+RGHYSERKAAEL KIIVGVVE+CHSLGVMHRDLKPENFLLV
Sbjct: 172  VHIVMELCAGGELFDRIIQRGHYSERKAAELTKIIVGVVEACHSLGVMHRDLKPENFLLV 231

Query: 1195 NKDDDFSLKAIDFGLSVFFKPGQVFTDVVGSPYYVAPEVLLKHYGQEADVWTAGVILYIL 1016
            NKDDDFSLKAIDFGLSVFFKPGQVFTDVVGSPYYVAPEVL KHYG EADVWTAGVILYIL
Sbjct: 232  NKDDDFSLKAIDFGLSVFFKPGQVFTDVVGSPYYVAPEVLCKHYGPEADVWTAGVILYIL 291

Query: 1015 LSGVPPFWAETQQGIFDAVLKGHIDFESDPWPVISDSAKDLIRKMLCSRPSDRLTAHEVL 836
            LSGVPPFWAETQQGIFDAVLKG IDFES+PWP+ISDSAKDLIRKMLCSRP+DRLTAHEVL
Sbjct: 292  LSGVPPFWAETQQGIFDAVLKGFIDFESEPWPLISDSAKDLIRKMLCSRPADRLTAHEVL 351

Query: 835  CHPWICENGVAPDRSLDPAVLSRLKQFSAMNKLKKMALRVIAESLSEEEIAGLKEMFSAM 656
            CHPWICENGVAPDRSLDPAVLSRLKQFSAMNKLKKMALRVIAESLSEEEIAGL+EMF AM
Sbjct: 352  CHPWICENGVAPDRSLDPAVLSRLKQFSAMNKLKKMALRVIAESLSEEEIAGLREMFKAM 411

Query: 655  DTDNSGAITFDELKAGLRKYGSTLKDTEIRDLMDAADVDNSGTIDYGEFVTATLHLNKLE 476
            DTD+SGAITFDELKAGLR+YGSTLK++EIRDLMDAADVDNSGTIDYGEF+ AT+HLNKLE
Sbjct: 412  DTDSSGAITFDELKAGLRRYGSTLKESEIRDLMDAADVDNSGTIDYGEFIAATVHLNKLE 471

Query: 475  REEHLVAAFQYFDKDSSGYITVDELQQACAEHNMTDVFLEDIIKEVDQDNDGRIDYGEFV 296
            REEHLVAAFQYFDKD SGYITVDELQQACAEHNMTDVFLEDIIKEVDQDNDGRIDY EFV
Sbjct: 472  REEHLVAAFQYFDKDGSGYITVDELQQACAEHNMTDVFLEDIIKEVDQDNDGRIDYSEFV 531

Query: 295  AMMQKGNAGIGRRTMRNSVNMSFRDAHGA 209
            AMMQKGNAGIGRRTMRNS+NMS RDA GA
Sbjct: 532  AMMQKGNAGIGRRTMRNSLNMSMRDAPGA 560


>ref|XP_002520611.1| calcium-dependent protein kinase, putative [Ricinus communis]
            gi|223540210|gb|EEF41784.1| calcium-dependent protein
            kinase, putative [Ricinus communis]
          Length = 561

 Score =  955 bits (2468), Expect = 0.0
 Identities = 479/569 (84%), Positives = 509/569 (89%), Gaps = 1/569 (0%)
 Frame = -2

Query: 1912 MGNSCSGSFGVKEYQGCTQPEDRSKCXXXXXXXXXXXXXXSEYSPTTLTAQQLVAQEFVK 1733
            MGN+C GSF  K YQG +QPED+S                 E+SPT++TAQ     EF K
Sbjct: 1    MGNTCRGSFKGKLYQGYSQPEDQSTASHTNRNPSSNNSNT-EHSPTSVTAQ-----EFAK 54

Query: 1732 DDQTIPKKDHTLPIVSP-KKDNTMSRSGNNYSCYVLGHATPNIRDVYTIGRKLGQGQFGI 1556
            D+   PKKD+  P++SP +KD  M R  +N S YVLGH T NIRD+YT+GRKLGQGQFG 
Sbjct: 55   DN---PKKDNNFPLISPNRKDLIMRRGADNQSYYVLGHKTANIRDLYTLGRKLGQGQFGT 111

Query: 1555 TYLCTEIATGIEYACKSISKRKLISREDVEDVRREIQIMHHLAGHKNIVTIKGAYEDHLY 1376
            TYLC E +TG EYACKSISKRKLIS+EDVEDVRREIQIMHHLAGHKNIVTIKGAYED LY
Sbjct: 112  TYLCVENSTGTEYACKSISKRKLISKEDVEDVRREIQIMHHLAGHKNIVTIKGAYEDQLY 171

Query: 1375 VHIVMELCNGGELFDRIIERGHYSERKAAELIKIIVGVVESCHSLGVMHRDLKPENFLLV 1196
            VHIVMELC+GGELFDRII+RGHYSERKAAEL KIIVGVVE+CHSLGVMHRDLKPENFLLV
Sbjct: 172  VHIVMELCSGGELFDRIIQRGHYSERKAAELTKIIVGVVEACHSLGVMHRDLKPENFLLV 231

Query: 1195 NKDDDFSLKAIDFGLSVFFKPGQVFTDVVGSPYYVAPEVLLKHYGQEADVWTAGVILYIL 1016
            NKDDDFSLKAIDFGLSVFFKPGQ+FTDVVGSPYYVAPEVLLKHYG EADVWTAGVILYIL
Sbjct: 232  NKDDDFSLKAIDFGLSVFFKPGQIFTDVVGSPYYVAPEVLLKHYGPEADVWTAGVILYIL 291

Query: 1015 LSGVPPFWAETQQGIFDAVLKGHIDFESDPWPVISDSAKDLIRKMLCSRPSDRLTAHEVL 836
            LSGVPPFWAETQQGIFDAVLKG+IDFESDPWP+ISDSAKDLI KMLC RPSDRLTAHEVL
Sbjct: 292  LSGVPPFWAETQQGIFDAVLKGYIDFESDPWPLISDSAKDLIHKMLCGRPSDRLTAHEVL 351

Query: 835  CHPWICENGVAPDRSLDPAVLSRLKQFSAMNKLKKMALRVIAESLSEEEIAGLKEMFSAM 656
            CHPWICENGVAPDR+LDPAVLSRLKQFSAMNKLKKMALRVIAESLSEEEIAGL+EMF  M
Sbjct: 352  CHPWICENGVAPDRALDPAVLSRLKQFSAMNKLKKMALRVIAESLSEEEIAGLREMFQTM 411

Query: 655  DTDNSGAITFDELKAGLRKYGSTLKDTEIRDLMDAADVDNSGTIDYGEFVTATLHLNKLE 476
            DTDNSGAITFDELKAGLR+YGST+KDTEIRDLMDAADVDNSGTIDYGEF+ AT+HLNKLE
Sbjct: 412  DTDNSGAITFDELKAGLRRYGSTMKDTEIRDLMDAADVDNSGTIDYGEFIAATVHLNKLE 471

Query: 475  REEHLVAAFQYFDKDSSGYITVDELQQACAEHNMTDVFLEDIIKEVDQDNDGRIDYGEFV 296
            REEHLVAAFQYFDKD SGYITVDELQQACAEHNMTDV LEDII+EVDQDNDGRIDY EFV
Sbjct: 472  REEHLVAAFQYFDKDGSGYITVDELQQACAEHNMTDVLLEDIIREVDQDNDGRIDYSEFV 531

Query: 295  AMMQKGNAGIGRRTMRNSVNMSFRDAHGA 209
            AMMQKGNAGIGRRTMRNS+NMS RDA GA
Sbjct: 532  AMMQKGNAGIGRRTMRNSLNMSMRDAPGA 560


>emb|CAN78387.1| hypothetical protein VITISV_017368 [Vitis vinifera]
          Length = 561

 Score =  954 bits (2467), Expect = 0.0
 Identities = 480/569 (84%), Positives = 514/569 (90%), Gaps = 1/569 (0%)
 Frame = -2

Query: 1912 MGNSCSGSFGVKEYQGCTQPEDRSKCXXXXXXXXXXXXXXSEYSPTTLTAQQLVAQEFVK 1733
            MGN+C GSF  K +QG  QPE++S                 +YSP++L +QQLV+QEF K
Sbjct: 1    MGNTCRGSFRGKYFQGYGQPEEQSTSKRNSDRSNS------DYSPSSLNSQQLVSQEFAK 54

Query: 1732 DDQTIPKKDHTLPIVSP-KKDNTMSRSGNNYSCYVLGHATPNIRDVYTIGRKLGQGQFGI 1556
            ++   PKK+  + I+SP  KD TM R  +N S YVLGH T NIRD+Y++GRKLGQGQFG 
Sbjct: 55   EN---PKKETRVSILSPTNKDGTMRRGVDNQSYYVLGHKTANIRDLYSLGRKLGQGQFGT 111

Query: 1555 TYLCTEIATGIEYACKSISKRKLISREDVEDVRREIQIMHHLAGHKNIVTIKGAYEDHLY 1376
            T LCT++ATGIEYACKSISKRKLISREDVEDVRREIQIMHHLAGHKNIVTIKGAYED LY
Sbjct: 112  TXLCTDMATGIEYACKSISKRKLISREDVEDVRREIQIMHHLAGHKNIVTIKGAYEDPLY 171

Query: 1375 VHIVMELCNGGELFDRIIERGHYSERKAAELIKIIVGVVESCHSLGVMHRDLKPENFLLV 1196
            VHIVMELC GGELFDRII+RGHYSER+AAEL KIIVGVVE+CHSLGVMHRDLKPENFLLV
Sbjct: 172  VHIVMELCAGGELFDRIIQRGHYSERRAAELTKIIVGVVEACHSLGVMHRDLKPENFLLV 231

Query: 1195 NKDDDFSLKAIDFGLSVFFKPGQVFTDVVGSPYYVAPEVLLKHYGQEADVWTAGVILYIL 1016
            NKDDDFSLKAIDFGLSVFFKPGQVFTDVVGSPYYVAPEVL KHYG EADVWTAGVILYIL
Sbjct: 232  NKDDDFSLKAIDFGLSVFFKPGQVFTDVVGSPYYVAPEVLCKHYGPEADVWTAGVILYIL 291

Query: 1015 LSGVPPFWAETQQGIFDAVLKGHIDFESDPWPVISDSAKDLIRKMLCSRPSDRLTAHEVL 836
            LSGVPPFWAETQQGIFDAVLKG IDFES+PWP+ISDSAKDLIRKMLCSRP+DRLTAHEVL
Sbjct: 292  LSGVPPFWAETQQGIFDAVLKGFIDFESEPWPLISDSAKDLIRKMLCSRPADRLTAHEVL 351

Query: 835  CHPWICENGVAPDRSLDPAVLSRLKQFSAMNKLKKMALRVIAESLSEEEIAGLKEMFSAM 656
            CHPWICENGVAPDRSLDPAVLSRLKQFSAMNKLKKMALRVIAESLSEEEIAGL+EMF AM
Sbjct: 352  CHPWICENGVAPDRSLDPAVLSRLKQFSAMNKLKKMALRVIAESLSEEEIAGLREMFKAM 411

Query: 655  DTDNSGAITFDELKAGLRKYGSTLKDTEIRDLMDAADVDNSGTIDYGEFVTATLHLNKLE 476
            DTD+SGAITFDELKAGLR+YGSTLK++EIRDLMDAADVDNSGTIDYGEF+ AT+HLNKLE
Sbjct: 412  DTDSSGAITFDELKAGLRRYGSTLKESEIRDLMDAADVDNSGTIDYGEFIAATVHLNKLE 471

Query: 475  REEHLVAAFQYFDKDSSGYITVDELQQACAEHNMTDVFLEDIIKEVDQDNDGRIDYGEFV 296
            REEHLVAAFQYFDKD SGYITVDELQQACAEHNMTDVFLEDIIKEVDQDNDGRIDY EFV
Sbjct: 472  REEHLVAAFQYFDKDGSGYITVDELQQACAEHNMTDVFLEDIIKEVDQDNDGRIDYSEFV 531

Query: 295  AMMQKGNAGIGRRTMRNSVNMSFRDAHGA 209
            AMMQKGNAGIGRRTMRNS+NMS RDA GA
Sbjct: 532  AMMQKGNAGIGRRTMRNSLNMSMRDAPGA 560


>gb|AAL68971.1| phloem calmodulin-like-domain protein kinase PCPK1 [Cucurbita maxima]
          Length = 571

 Score =  951 bits (2458), Expect = 0.0
 Identities = 470/570 (82%), Positives = 509/570 (89%), Gaps = 2/570 (0%)
 Frame = -2

Query: 1912 MGNSCSGSFGVKEYQGCTQPEDRSKCXXXXXXXXXXXXXXSEYSPTTLTAQQLVAQEFVK 1733
            MGN+C GSF    +QG +QPED S                S+ SPT++    L+AQEF K
Sbjct: 1    MGNTCRGSFKGNIFQGYSQPEDSSTLYSKRNNSSSSARSNSDISPTSINTHNLIAQEFSK 60

Query: 1732 DDQTIPK-KDHTLPIVSP-KKDNTMSRSGNNYSCYVLGHATPNIRDVYTIGRKLGQGQFG 1559
            +++T  K  D+    +SP KKD TM +S  N + YVLGH T NIRD+YT+GRKLGQGQFG
Sbjct: 61   ENKTKSKDNDYNQAFLSPTKKDFTMRKSAENQAYYVLGHKTANIRDLYTLGRKLGQGQFG 120

Query: 1558 ITYLCTEIATGIEYACKSISKRKLISREDVEDVRREIQIMHHLAGHKNIVTIKGAYEDHL 1379
             TYLCTEI TGIEYACKSISKRKLI++EDVEDVRREIQIMHHLAGHKNIVTIKGAYED L
Sbjct: 121  TTYLCTEITTGIEYACKSISKRKLIAKEDVEDVRREIQIMHHLAGHKNIVTIKGAYEDSL 180

Query: 1378 YVHIVMELCNGGELFDRIIERGHYSERKAAELIKIIVGVVESCHSLGVMHRDLKPENFLL 1199
            YVHIVMELC+GGELFDRII RGHYSERKAAEL +IIVGVVE+CHSLGVMHRDLKPENFLL
Sbjct: 181  YVHIVMELCSGGELFDRIIHRGHYSERKAAELTRIIVGVVETCHSLGVMHRDLKPENFLL 240

Query: 1198 VNKDDDFSLKAIDFGLSVFFKPGQVFTDVVGSPYYVAPEVLLKHYGQEADVWTAGVILYI 1019
            VNKDDDFSLKAIDFGLSVFFKPGQ+FTDVVG PYYVAPEVLLKHYG  ADVWTAGVILYI
Sbjct: 241  VNKDDDFSLKAIDFGLSVFFKPGQIFTDVVGRPYYVAPEVLLKHYGPAADVWTAGVILYI 300

Query: 1018 LLSGVPPFWAETQQGIFDAVLKGHIDFESDPWPVISDSAKDLIRKMLCSRPSDRLTAHEV 839
            LLSGVPPFWAETQQGIFDAVLKGHIDF+SDPWP+ISDSAKD+IRKMLCSRPSDRLTAHEV
Sbjct: 301  LLSGVPPFWAETQQGIFDAVLKGHIDFDSDPWPLISDSAKDIIRKMLCSRPSDRLTAHEV 360

Query: 838  LCHPWICENGVAPDRSLDPAVLSRLKQFSAMNKLKKMALRVIAESLSEEEIAGLKEMFSA 659
            LCHPWICENGVAPDR+LDPAVLSRLKQFSAMNKLKKMALRVIAESLSEEEIAGL+EMF+A
Sbjct: 361  LCHPWICENGVAPDRALDPAVLSRLKQFSAMNKLKKMALRVIAESLSEEEIAGLREMFTA 420

Query: 658  MDTDNSGAITFDELKAGLRKYGSTLKDTEIRDLMDAADVDNSGTIDYGEFVTATLHLNKL 479
            MDTDNSGAITFDELKAGLR+YGSTLKD EIRDLMDAAD+DNSGTIDYGEF+ AT+HLNKL
Sbjct: 421  MDTDNSGAITFDELKAGLRRYGSTLKDIEIRDLMDAADIDNSGTIDYGEFIAATIHLNKL 480

Query: 478  EREEHLVAAFQYFDKDSSGYITVDELQQACAEHNMTDVFLEDIIKEVDQDNDGRIDYGEF 299
            EREEHLVAAF+YFDKD SGYITVDELQQACAEHNMTD +LED+I+EVDQDNDGRIDYGEF
Sbjct: 481  EREEHLVAAFRYFDKDGSGYITVDELQQACAEHNMTDAYLEDVIREVDQDNDGRIDYGEF 540

Query: 298  VAMMQKGNAGIGRRTMRNSVNMSFRDAHGA 209
            VAMMQKGNAG+GRRTMRNS+N+S RD  GA
Sbjct: 541  VAMMQKGNAGVGRRTMRNSLNLSMRDGPGA 570


>ref|XP_004144896.1| PREDICTED: calcium-dependent protein kinase 4-like [Cucumis sativus]
            gi|449471982|ref|XP_004153460.1| PREDICTED:
            calcium-dependent protein kinase 4-like [Cucumis sativus]
            gi|449530458|ref|XP_004172212.1| PREDICTED:
            calcium-dependent protein kinase 4-like [Cucumis sativus]
          Length = 566

 Score =  949 bits (2453), Expect = 0.0
 Identities = 471/570 (82%), Positives = 513/570 (90%), Gaps = 2/570 (0%)
 Frame = -2

Query: 1912 MGNSCSGSFGVKEYQGCTQPEDRSKCXXXXXXXXXXXXXXSEYSPTTLTAQQLVAQEFVK 1733
            MGN+C GSF    +QG +QPED S                +++SP++++   L++QEF K
Sbjct: 1    MGNTCRGSFKGNIFQGYSQPEDSST-----PNSKRNTNTSADHSPSSISTNNLISQEFAK 55

Query: 1732 DDQTIPKKDHTLP-IVSP-KKDNTMSRSGNNYSCYVLGHATPNIRDVYTIGRKLGQGQFG 1559
            +++   K +   P  +SP KKD TM +S  N + YVLGH T NIRD+YT+GRKLGQGQFG
Sbjct: 56   ENKIKSKDNDCNPTFLSPTKKDYTMRKSAENQAYYVLGHKTANIRDLYTLGRKLGQGQFG 115

Query: 1558 ITYLCTEIATGIEYACKSISKRKLISREDVEDVRREIQIMHHLAGHKNIVTIKGAYEDHL 1379
             TYLCTEI TGIEYACKSISKRKLI++EDVEDVRREIQIMHHLAGHKNIVTIKGAYED L
Sbjct: 116  TTYLCTEITTGIEYACKSISKRKLIAKEDVEDVRREIQIMHHLAGHKNIVTIKGAYEDSL 175

Query: 1378 YVHIVMELCNGGELFDRIIERGHYSERKAAELIKIIVGVVESCHSLGVMHRDLKPENFLL 1199
            YVHIVMELC+GGELFDRII+RGHYSERKAAEL +IIVGVVE+CHSLGVMHRDLKPENFLL
Sbjct: 176  YVHIVMELCSGGELFDRIIQRGHYSERKAAELTRIIVGVVETCHSLGVMHRDLKPENFLL 235

Query: 1198 VNKDDDFSLKAIDFGLSVFFKPGQVFTDVVGSPYYVAPEVLLKHYGQEADVWTAGVILYI 1019
            VNKDDDFSLKAIDFGLSVFFKPGQ+FTDVVGSPYYVAPEVLLKHYG  ADVWTAGVILYI
Sbjct: 236  VNKDDDFSLKAIDFGLSVFFKPGQIFTDVVGSPYYVAPEVLLKHYGPAADVWTAGVILYI 295

Query: 1018 LLSGVPPFWAETQQGIFDAVLKGHIDFESDPWPVISDSAKDLIRKMLCSRPSDRLTAHEV 839
            LLSGVPPFWAETQQGIFDAVLKGHIDF+SDPWP+ISDSAKDLIRKMLCSRPSDRLTAHEV
Sbjct: 296  LLSGVPPFWAETQQGIFDAVLKGHIDFDSDPWPLISDSAKDLIRKMLCSRPSDRLTAHEV 355

Query: 838  LCHPWICENGVAPDRSLDPAVLSRLKQFSAMNKLKKMALRVIAESLSEEEIAGLKEMFSA 659
            LCHPWICENGVAPDR+LDPAVLSRLKQFSAMNKLKKMALRVIAESLSEEEIAGL+EMF+A
Sbjct: 356  LCHPWICENGVAPDRALDPAVLSRLKQFSAMNKLKKMALRVIAESLSEEEIAGLREMFTA 415

Query: 658  MDTDNSGAITFDELKAGLRKYGSTLKDTEIRDLMDAADVDNSGTIDYGEFVTATLHLNKL 479
            MDTDNSGAITFDELKAGLR+YGSTLKD EIRDLMDAAD+DNSGTIDYGEF+ AT+HLNKL
Sbjct: 416  MDTDNSGAITFDELKAGLRRYGSTLKDIEIRDLMDAADIDNSGTIDYGEFIAATIHLNKL 475

Query: 478  EREEHLVAAFQYFDKDSSGYITVDELQQACAEHNMTDVFLEDIIKEVDQDNDGRIDYGEF 299
            EREEHLVAAF+YFDKD SGYITVDELQQACAEHNMTDV+LEDII+EVDQDNDGRIDYGEF
Sbjct: 476  EREEHLVAAFRYFDKDGSGYITVDELQQACAEHNMTDVYLEDIIREVDQDNDGRIDYGEF 535

Query: 298  VAMMQKGNAGIGRRTMRNSVNMSFRDAHGA 209
            VAMMQKGNAGIGRRTMRNS+N+S RD  GA
Sbjct: 536  VAMMQKGNAGIGRRTMRNSLNLSMRDGPGA 565