BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00000233
         (1502 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI33881.3| unnamed protein product [Vitis vinifera]              308   3e-81
ref|XP_004165982.1| PREDICTED: ubiquitin carboxyl-terminal hydro...   248   4e-63
ref|XP_004148760.1| PREDICTED: ubiquitin carboxyl-terminal hydro...   246   1e-62
ref|XP_002329560.1| predicted protein [Populus trichocarpa] gi|2...   237   7e-60
ref|XP_002326526.1| predicted protein [Populus trichocarpa] gi|2...   228   2e-57

>emb|CBI33881.3| unnamed protein product [Vitis vinifera]
          Length = 547

 Score =  308 bits (788), Expect = 3e-81
 Identities = 177/367 (48%), Positives = 228/367 (62%), Gaps = 11/367 (2%)
 Frame = +1

Query: 4    KFTCENCKEQVSIEKQLILEETPSVAAFHLKRFKNDGSYVEKIEKFVEFPLELDLQPYTN 183
            KFTC+NCKE+VS+EKQL+L++ P VAAFHLKRFK DG+YVEKI+K VEFPLELDL PYT 
Sbjct: 145  KFTCDNCKEEVSVEKQLLLDQVPLVAAFHLKRFKTDGTYVEKIDKHVEFPLELDLLPYTK 204

Query: 184  ICQNSDVELKYELYAIVVHIGFSATSGHYYSFIRSAPDTWYKFDDSQVVRVSEGFVMSQD 363
              ++ +VELKYELYAIV H GFS+TSGHY+S+IRS+PDTW++ DDS+V  V E +V+ Q+
Sbjct: 205  EREDDNVELKYELYAIVEHTGFSSTSGHYFSYIRSSPDTWHRLDDSKVTSVPEDYVLHQE 264

Query: 364  AYILFYAKDGTPWFSTYLENIKPFLDPHSSNTSPKSVLDNVD-IPTSSPNNVTEPSHTAH 540
            AYILFYA+ GTPWFS+ ++  KP LDP++S  SPKSVLDNVD I TS P      SH A 
Sbjct: 265  AYILFYARQGTPWFSSLMQTQKPCLDPNTSGNSPKSVLDNVDSISTSLPTLANVYSHEAS 324

Query: 541  ESIFESPSVS-------RHDQEDNIECKDDTQRMST-FPLGATSPVDNAVDGSQDNDVTP 696
            E++  +  +S       R D     E  D   R+ T  PLG TS  D  +D +Q      
Sbjct: 325  ETVDAADEISTCLSGGGRGDVVQVHEDGDVPHRVDTPMPLGVTSCFDGTLDHTQ------ 378

Query: 697  NVATASPPGTTILLKDNCNGMKVFSPSVLQEHSLNQ--DDVKISSDNPQTPPRSSSPDIY 870
            N++T SP                      ++H  NQ  ++   S  +PQTPPRS SPDIY
Sbjct: 379  NISTTSP---------------------CRDHDGNQHINENCKSKIHPQTPPRSPSPDIY 417

Query: 871  EDESPDAESNINVKNHKLLNQGFGKSQLKIEAESFETKQAQRLFRSLPARRSKPLSAVMS 1050
             +E P+   NI   + +  NQ   K  L    E    ++A +L +++PA RS  L A M 
Sbjct: 418  AEEPPEVSYNIPRGHLRQKNQVACKKPLNKALEDSRKREACKLCKNMPASRSMRLMAAMK 477

Query: 1051 GSLSEDS 1071
            GS SE S
Sbjct: 478  GSQSEGS 484


>ref|XP_004165982.1| PREDICTED: ubiquitin carboxyl-terminal hydrolase 20-like [Cucumis
            sativus]
          Length = 621

 Score =  248 bits (632), Expect = 4e-63
 Identities = 158/385 (41%), Positives = 213/385 (55%), Gaps = 5/385 (1%)
 Frame = +1

Query: 1    TKFTCENCKEQVSIEKQLILEETPSVAAFHLKRFKNDGSYVEKIEKFVEFPLELDLQPYT 180
            TKF CENCKE+V +EKQL LE+ P V  FHLKRFK D S+VEKIEK +EFPLELDL+PYT
Sbjct: 266  TKFKCENCKEEVWVEKQLKLEKIPLVTTFHLKRFKADSSFVEKIEKHLEFPLELDLKPYT 325

Query: 181  NICQNSDVELKYELYAIVVHIGFSATSGHYYSFIRSAPDTWYKFDDSQVVRVSEGFVMSQ 360
               ++++VELKYELYAIV H GFS+TSGHY+SF+RS+PDTW++ DDS V  V+E +V+S+
Sbjct: 326  LGGKDAEVELKYELYAIVEHTGFSSTSGHYFSFVRSSPDTWHRMDDSLVTSVTEEYVLSR 385

Query: 361  DAYILFYAKDGTPWFSTYLENIKPFLDPHSSNTSPKSVLDNVDIPTSSPNNVTEPSHTAH 540
            +AYIL Y + GT WFS+ ++   P L+  SSN SPKSVL N+    SS       S   H
Sbjct: 386  EAYILLYKRKGTSWFSSLIQRSDPCLNSDSSNPSPKSVLQNIGTGCSS---FAAESKVNH 442

Query: 541  ESIFESPSVSRHDQEDNIECKDDTQRMSTFPLGATSPVDNAVDGSQDNDVTPNVATASPP 720
            ES   +P+ +         C+ + Q          S ++NA   S + ++  +  T S P
Sbjct: 443  ESA-NAPADTLQPSPARFSCEMENQDT------GVSDLENAT--SANLNIVESATTKSSP 493

Query: 721  GTTILLKDNCNGMKVFSPSVLQEHSLNQDDVKISSDNPQTPPRSSSPDIYEDESPDAESN 900
               I+ KD C               LN +D  I   +P +P RS SPD     +P+ +  
Sbjct: 494  ---IVTKDLC---------------LNDEDESIDGFHPLSPVRSPSPD-NVFRTPEPQYR 534

Query: 901  INVKNHKLLNQGFGKSQLKIEAESFETKQAQR-LFRSLPARRSKPLSAVMSGSLSEDS-- 1071
            I V + K        S L    E  E K A R + +++P  R   + A + GS SE S  
Sbjct: 535  IPVDHLKTEKCSTSGSPLNKGVEDSERKAAMRYVKKNMPGSRGSKMIAAILGSQSEGSSL 594

Query: 1072 --QXXXXXXXXXXXSPSTTQRKASL 1140
              +           SP  T+RK S+
Sbjct: 595  NKKKRLRSLPCKRSSPPNTRRKTSI 619


>ref|XP_004148760.1| PREDICTED: ubiquitin carboxyl-terminal hydrolase 20-like [Cucumis
            sativus]
          Length = 676

 Score =  246 bits (627), Expect = 1e-62
 Identities = 156/385 (40%), Positives = 212/385 (55%), Gaps = 5/385 (1%)
 Frame = +1

Query: 1    TKFTCENCKEQVSIEKQLILEETPSVAAFHLKRFKNDGSYVEKIEKFVEFPLELDLQPYT 180
            TKF CENCKE+V +EKQL LE+ P V  FHLKRFK D S+VEKIEK +EFPLELDL+PYT
Sbjct: 321  TKFKCENCKEEVWVEKQLKLEKIPLVTTFHLKRFKADSSFVEKIEKHLEFPLELDLKPYT 380

Query: 181  NICQNSDVELKYELYAIVVHIGFSATSGHYYSFIRSAPDTWYKFDDSQVVRVSEGFVMSQ 360
               ++++VELKYELYAIV H GFS+TSGHY+SF+RS+PDTW++ DDS V  V+E +V+S+
Sbjct: 381  LGGKDAEVELKYELYAIVEHTGFSSTSGHYFSFVRSSPDTWHRMDDSLVTSVTEEYVLSR 440

Query: 361  DAYILFYAKDGTPWFSTYLENIKPFLDPHSSNTSPKSVLDNVDIPTSSPNNVTEPSHTAH 540
            +AYIL Y + GT WFS+ ++   P L+  SSN SPKSVL N+    SS       S   H
Sbjct: 441  EAYILLYKRKGTSWFSSLIQRSDPCLNSDSSNPSPKSVLQNIGTGCSS---FAAESKVNH 497

Query: 541  ESIFESPSVSRHDQEDNIECKDDTQRMSTFPLGATSPVDNAVDGSQDNDVTPNVATASPP 720
            ES   +P+ +         C+ + Q          S ++NA   + +      V +A+  
Sbjct: 498  ESA-NAPADTLQPSPARFSCEMENQDT------GVSDLENATSANLN-----IVESATMK 545

Query: 721  GTTILLKDNCNGMKVFSPSVLQEHSLNQDDVKISSDNPQTPPRSSSPDIYEDESPDAESN 900
             + I+ KD C               LN +D  I   +P +P RS SPD     +P+ +  
Sbjct: 546  SSPIVTKDLC---------------LNDEDESIDGFHPLSPVRSPSPD-NVFRTPEPQYR 589

Query: 901  INVKNHKLLNQGFGKSQLKIEAESFETKQAQR-LFRSLPARRSKPLSAVMSGSLSEDS-- 1071
            I V + K        S L    E  E K A R + +++P  R   + A + GS SE S  
Sbjct: 590  IPVDHLKTEKCSTSGSPLNKGVEDSERKAAMRYVKKNMPGSRGSKMIAAILGSQSEGSSL 649

Query: 1072 --QXXXXXXXXXXXSPSTTQRKASL 1140
              +           SP  T+RK S+
Sbjct: 650  NKKKRLRSLPCKRSSPPNTRRKTSI 674


>ref|XP_002329560.1| predicted protein [Populus trichocarpa] gi|222870269|gb|EEF07400.1|
            predicted protein [Populus trichocarpa]
          Length = 809

 Score =  237 bits (604), Expect = 7e-60
 Identities = 149/354 (42%), Positives = 204/354 (57%), Gaps = 5/354 (1%)
 Frame = +1

Query: 1    TKFTCENCKEQVSIEKQLILEETPSVAAFHLKRFKNDGSYVEKIEKFVEFPLELDLQPYT 180
            TKFTCENCKE+VS EKQL+L++ PS+AA HLKRFK DG+ VEKI K VEFPLELDL+PY 
Sbjct: 441  TKFTCENCKEEVSREKQLMLDKAPSIAALHLKRFKIDGTSVEKIGKHVEFPLELDLKPYI 500

Query: 181  NICQNSDVELKYELYAIVVHIGFSATSGHYYSFIRSAPDTWYKFDDSQVVRVSEGFVMSQ 360
            N   ++DV  KY+LYA+VVH G+S TSGHY  +IRS+PDTW+K DD +V +  E FV+SQ
Sbjct: 501  NDNDSNDVGFKYQLYAVVVHKGYSLTSGHYVCYIRSSPDTWHKLDDPEVSKEREEFVLSQ 560

Query: 361  DAYILFYAKDGTPWFSTYLENIKPFLDPHSSNTSPKSVLDNVDIPTSSPNNVTEPSHTAH 540
             AYILFYA++GTPW S+ ++  +  LD  +SNTSPKSVLDN +    S  +      +  
Sbjct: 561  AAYILFYAREGTPWCSSLIKPQEFCLDRSNSNTSPKSVLDNANSECISVADKDSLETSVI 620

Query: 541  ESIFESPS--VSRHDQEDNIECKDDTQRMSTFPLGATSPVDNAVDGSQDNDVTPNVATAS 714
            +   E+ S  +    + + I  + +T+ +S F +       N VD S  + V+       
Sbjct: 621  KDAVEATSTHIPCEREFEEIVSRGETEGIS-FQISCA----NMVDVSLPHGVS------- 668

Query: 715  PPGTTILLKDNCNGMKVFSPSVLQEHSLNQDDVKISSD---NPQTPPRSSSPDIYEDESP 885
              G   +L D    M  F P  ++E   NQ   KI       P  PPRS + D+Y  E P
Sbjct: 669  -DGHDGVLHDE---MLCFPP--VEEDYSNQCAEKIGRKGDLRPSAPPRSPTSDVYSGELP 722

Query: 886  DAESNINVKNHKLLNQGFGKSQLKIEAESFETKQAQRLFRSLPARRSKPLSAVM 1047
            +A   I  ++ K+ N    K   K   +  +T +A R  + +P  R   L A +
Sbjct: 723  EARRCIPREHLKVENHVNCKRSSKKGTKDSQTAEALRCIKRMPTARGMKLMAAL 776


>ref|XP_002326526.1| predicted protein [Populus trichocarpa] gi|222833848|gb|EEE72325.1|
            predicted protein [Populus trichocarpa]
          Length = 835

 Score =  228 bits (582), Expect = 2e-57
 Identities = 154/366 (42%), Positives = 214/366 (58%), Gaps = 17/366 (4%)
 Frame = +1

Query: 1    TKFTCENCKEQVSIEKQLILEETPSVAAFHLKRFKNDGSYVEKIEKFVEFPLELDLQPYT 180
            TKFTCE+CKE+VS EKQL+L++ PSVAA HLK FK DG+ VEKI K V+FPLELDL+PYT
Sbjct: 453  TKFTCESCKEEVSREKQLMLDQAPSVAALHLKIFKIDGTSVEKIGKHVQFPLELDLKPYT 512

Query: 181  NICQNSD-VELKYELYAIVVHIGFSATSGHYYSFIRSAPDTWYKFDDSQVVRVSEGFVMS 357
            N  ++SD V  KY+LYA+VVH G S  SGHY+ +IRS+PDTW+K DD +V +  E FV+S
Sbjct: 513  NDNEDSDEVGFKYQLYAVVVHKGDSLMSGHYFCYIRSSPDTWHKLDDPEVSKEQEEFVLS 572

Query: 358  QDAYILFYAKDGTPWFSTYLENIKPFLDPHSSNTSPKSVLDNVDIP-TSSPNNVTEPSHT 534
            Q AYILFYA++GTPW S+ ++  +   DP +SNTSPKSVLDNV+   T   NN +  ++ 
Sbjct: 573  QAAYILFYAREGTPWCSSLIKPQELCSDPSNSNTSPKSVLDNVNRECTGVGNNKSSETNV 632

Query: 535  AHESIFESPSVSRH--------DQEDNIECKDDTQRMSTFPLGATSPVDNAVDGSQDNDV 690
              ++I    + S H        + E  +E K +  ++S     A  P  + +    +   
Sbjct: 633  IKDAI---EATSTHIPFERKFEESESRVETKGNFVQISP----ANRPNFHRIVSIGE--- 682

Query: 691  TPNVATASPPGTTI----LLKDNCNGMKVFSPSVLQEHSLNQ--DDVKISSD-NPQTPPR 849
            TP V  + P G +     +L D    M  F PSV +E + NQ  + ++I+ D +  TP R
Sbjct: 683  TPMVDVSVPLGVSDYHDGVLHDE---MLCFPPSV-EEDNCNQGAEKIEINGDLHSPTPHR 738

Query: 850  SSSPDIYEDESPDAESNINVKNHKLLNQGFGKSQLKIEAESFETKQAQRLFRSLPARRSK 1029
            S +PD      P+A   I   + K  N+   K   K   +  +T +A R  + +P  R  
Sbjct: 739  SPTPD---KGLPEARHRILRDHQKGENRVNCKRSSKKVTKDSQTAEALRCIKRMPTARGM 795

Query: 1030 PLSAVM 1047
             L A M
Sbjct: 796  KLMAAM 801