BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00000180
         (1723 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002265086.1| PREDICTED: vacuolar proton translocating ATP...   719   0.0  
ref|XP_002512965.1| vacuolar proton atpase, putative [Ricinus co...   709   0.0  
emb|CAB38828.1| putative proton pump [Arabidopsis thaliana] gi|7...   698   0.0  
ref|NP_568051.1| V-type H+-transporting ATPase subunit I [Arabid...   698   0.0  
ref|XP_002866830.1| VHA-A3 [Arabidopsis lyrata subsp. lyrata] gi...   695   0.0  

>ref|XP_002265086.1| PREDICTED: vacuolar proton translocating ATPase 100 kDa subunit
            [Vitis vinifera] gi|297744757|emb|CBI38019.3| unnamed
            protein product [Vitis vinifera]
          Length = 822

 Score =  719 bits (1856), Expect(2) = 0.0
 Identities = 349/460 (75%), Positives = 404/460 (87%)
 Frame = +2

Query: 98   MAESGGDSGGCCPPMDLFRSEPMHLVQLILPIESAHLSVSYLGDLGLVQFKDLNSDKSPF 277
            M + GG  GGCCPPMDLFRSEPM LVQLI+PIESAH ++SYLGDLGL+QFKDLN +KSPF
Sbjct: 1    MGDGGGGRGGCCPPMDLFRSEPMQLVQLIIPIESAHHTISYLGDLGLIQFKDLNVEKSPF 60

Query: 278  QRTYATQIKRCGEMARKLRFFRDQMLKAGVTPPSRSASEDDLDLDTLEVKLGQLEAELIE 457
            QRTYA QIK+C EMARKLRFF++QM KAG++P ++     D+D+D LEVKLG+LEAEL+E
Sbjct: 61   QRTYAAQIKKCAEMARKLRFFKEQMSKAGLSPSAKIMMRGDIDMDDLEVKLGELEAELVE 120

Query: 458  LNANSNKLQRGYNELEEYKLVLQKAGEFFHSALSNAEARQREAGSAQSGEESLETPLLEE 637
            +NAN  KLQR Y+EL EYKLVL KAGEFF+S  S+A A+QRE  +    EES++TPLL E
Sbjct: 121  INANGEKLQRAYSELAEYKLVLHKAGEFFYSIRSSATAQQREIEAHSISEESVDTPLLLE 180

Query: 638  QEMTTDQSKQVKLGFISGLVPKVKSLSFERILFRATRGNVYLKQAVVEHPVTDPVSGEKV 817
            QEM+TD SKQVKLGF++GLVP+VKS++FERILFRATRGNV+L+Q+ VE PVTDPVSGEK+
Sbjct: 181  QEMSTDLSKQVKLGFLAGLVPRVKSMAFERILFRATRGNVFLRQSAVEDPVTDPVSGEKI 240

Query: 818  EKNVFAVFFSGEKAKNKILKICEAFGANRYPFSEDISKQAHMITEVSGRISELKTTIDIG 997
            EKNVF VF+SGEK KNKILKICEAFGANRY F ED+ KQA MITEVSGR+SELKTTID+G
Sbjct: 241  EKNVFVVFYSGEKVKNKILKICEAFGANRYSFPEDLGKQAQMITEVSGRLSELKTTIDVG 300

Query: 998  SSQCENLLKTIGEDYERWNILVRREKSIYHTLNMLSIDVTKKCLVAEGWSPISATKKIQD 1177
                 NLL+TIG+ +E+WN+LVR+EKSIYHTLNMLSIDVTKKCLVAEGWSP  ATK+IQD
Sbjct: 301  LLHRGNLLQTIGDQFEQWNLLVRKEKSIYHTLNMLSIDVTKKCLVAEGWSPTFATKQIQD 360

Query: 1178 ALQRATIDSNSQIGGIFRVLHTKESPPTYFKTNKFTSSFQEIVDAYGIAKYQEANPGVYT 1357
            ALQRAT DSNSQ+G IF+VLHT ESPPTYF+TNKFTS+FQEIVDAYG+AKYQEANPGV+T
Sbjct: 361  ALQRATFDSNSQVGAIFQVLHTIESPPTYFRTNKFTSAFQEIVDAYGVAKYQEANPGVFT 420

Query: 1358 IVTFPFLFAVMFGDWGHGICLLLGTLYFIAMEKKFSSQKL 1477
            IVTFPFLFAVMFGDWGHG+CLLL TL+FI  EKK S+QKL
Sbjct: 421  IVTFPFLFAVMFGDWGHGLCLLLATLFFIIREKKLSNQKL 460



 Score =  104 bits (259), Expect(2) = 0.0
 Identities = 48/58 (82%), Positives = 53/58 (91%)
 Frame = +1

Query: 1546 MTFGGRYVILMMSLFSIYTGLIYNEFFSIPFELFSPSAYGCRDPTCGDSTTIGLIKVR 1719
            MTFGGRYVILMM+LFSIYTGLIYNEFFS+PFELF PSAY CRD +C D++T GLIKVR
Sbjct: 466  MTFGGRYVILMMALFSIYTGLIYNEFFSVPFELFGPSAYACRDLSCRDASTAGLIKVR 523


>ref|XP_002512965.1| vacuolar proton atpase, putative [Ricinus communis]
            gi|223547976|gb|EEF49468.1| vacuolar proton atpase,
            putative [Ricinus communis]
          Length = 814

 Score =  709 bits (1829), Expect(2) = 0.0
 Identities = 344/451 (76%), Positives = 400/451 (88%)
 Frame = +2

Query: 125  GCCPPMDLFRSEPMHLVQLILPIESAHLSVSYLGDLGLVQFKDLNSDKSPFQRTYATQIK 304
            GCCPPMDLFRSE M LVQLI+PIESAHL+VSYLGDLGL+QFKDLNS+KSPFQRTYA Q+K
Sbjct: 2    GCCPPMDLFRSEAMQLVQLIIPIESAHLTVSYLGDLGLLQFKDLNSEKSPFQRTYAAQLK 61

Query: 305  RCGEMARKLRFFRDQMLKAGVTPPSRSASEDDLDLDTLEVKLGQLEAELIELNANSNKLQ 484
            +CGEMARKLRFF+DQM KAGV P S+S + +D+++D L++KLG+LEAEL+E+NAN++KLQ
Sbjct: 62   KCGEMARKLRFFKDQMEKAGVFPSSKSTTRNDINMDGLDIKLGELEAELVEMNANNDKLQ 121

Query: 485  RGYNELEEYKLVLQKAGEFFHSALSNAEARQREAGSAQSGEESLETPLLEEQEMTTDQSK 664
            R YNEL EYKLVL KAGEFF SALS+A ++QRE  S Q GEESLETPLL +QE++TD SK
Sbjct: 122  RTYNELIEYKLVLHKAGEFFSSALSSATSQQRELESGQVGEESLETPLLGDQEISTDSSK 181

Query: 665  QVKLGFISGLVPKVKSLSFERILFRATRGNVYLKQAVVEHPVTDPVSGEKVEKNVFAVFF 844
            QVKLGF++GLVPK KS++FERI+FRATRGNV+L+QA VE PV DPVSGEK+EKNVF VFF
Sbjct: 182  QVKLGFLTGLVPKDKSIAFERIIFRATRGNVFLRQAAVEEPVIDPVSGEKIEKNVFVVFF 241

Query: 845  SGEKAKNKILKICEAFGANRYPFSEDISKQAHMITEVSGRISELKTTIDIGSSQCENLLK 1024
            SGEKAK KILKICEAFGANRYPF+ED+ KQ  MITEVSGR+SELKTTID G     NLL+
Sbjct: 242  SGEKAKTKILKICEAFGANRYPFTEDLGKQNQMITEVSGRLSELKTTIDAGLLHRSNLLR 301

Query: 1025 TIGEDYERWNILVRREKSIYHTLNMLSIDVTKKCLVAEGWSPISATKKIQDALQRATIDS 1204
            TI + + +WN +VR+EKS+YHTLNMLS+DVTKKCLVAE WSP+ A+K+IQ+AL RA  DS
Sbjct: 302  TIADQFVQWNSMVRKEKSVYHTLNMLSLDVTKKCLVAEAWSPVFASKQIQEALHRAAFDS 361

Query: 1205 NSQIGGIFRVLHTKESPPTYFKTNKFTSSFQEIVDAYGIAKYQEANPGVYTIVTFPFLFA 1384
            NSQ+G IF+VLH KESPPTYF+TNKFTS+FQEIVD+YG+AKYQEANPGV+TIVTFPFLFA
Sbjct: 362  NSQVGAIFQVLHAKESPPTYFRTNKFTSAFQEIVDSYGVAKYQEANPGVFTIVTFPFLFA 421

Query: 1385 VMFGDWGHGICLLLGTLYFIAMEKKFSSQKL 1477
            VMFGDWGHGICLLL TL FI  EKK SSQKL
Sbjct: 422  VMFGDWGHGICLLLATLVFIIREKKLSSQKL 452



 Score = 98.6 bits (244), Expect(2) = 0.0
 Identities = 46/57 (80%), Positives = 51/57 (89%)
 Frame = +1

Query: 1546 MTFGGRYVILMMSLFSIYTGLIYNEFFSIPFELFSPSAYGCRDPTCGDSTTIGLIKV 1716
            MTFGGRYVIL+M+LFSIYTGLIYNEFFS+PFELF  SAY CRD +C D+TT GLIKV
Sbjct: 458  MTFGGRYVILLMALFSIYTGLIYNEFFSVPFELFGRSAYACRDLSCRDATTDGLIKV 514


>emb|CAB38828.1| putative proton pump [Arabidopsis thaliana]
            gi|7270891|emb|CAB80571.1| putative proton pump
            [Arabidopsis thaliana]
          Length = 843

 Score =  698 bits (1802), Expect(2) = 0.0
 Identities = 343/460 (74%), Positives = 400/460 (86%)
 Frame = +2

Query: 98   MAESGGDSGGCCPPMDLFRSEPMHLVQLILPIESAHLSVSYLGDLGLVQFKDLNSDKSPF 277
            MAESGG  GGCCPPMDL RSE M LVQLI+P+ESAHL+VSYLGDLGLVQFKDLNS+KSPF
Sbjct: 1    MAESGG-GGGCCPPMDLMRSETMQLVQLIVPMESAHLTVSYLGDLGLVQFKDLNSEKSPF 59

Query: 278  QRTYATQIKRCGEMARKLRFFRDQMLKAGVTPPSRSASEDDLDLDTLEVKLGQLEAELIE 457
            QRTYA QIKRCGEMARK+RFFRDQM KAGV        E+D+DLD +EVKLG+LEAEL+E
Sbjct: 60   QRTYAAQIKRCGEMARKIRFFRDQMSKAGVPAKEMQGKENDIDLDDVEVKLGELEAELVE 119

Query: 458  LNANSNKLQRGYNELEEYKLVLQKAGEFFHSALSNAEARQREAGSAQSGEESLETPLLEE 637
            +NAN++KLQR YNEL EYKLVLQKAGEFF SA  +A  +QRE  S Q+GE+ LE+PLL+E
Sbjct: 120  INANNDKLQRSYNELMEYKLVLQKAGEFFSSAHRSAADQQRETESQQAGEDLLESPLLQE 179

Query: 638  QEMTTDQSKQVKLGFISGLVPKVKSLSFERILFRATRGNVYLKQAVVEHPVTDPVSGEKV 817
             E + D +KQVKLGF++GLVP+ KS+ FERILFRATRGN++++Q V+E PV DP SGEK 
Sbjct: 180  -EKSIDSTKQVKLGFLTGLVPREKSMVFERILFRATRGNIFIRQTVIEEPVIDPNSGEKA 238

Query: 818  EKNVFAVFFSGEKAKNKILKICEAFGANRYPFSEDISKQAHMITEVSGRISELKTTIDIG 997
            EKNVF VF+SGE+AK+KILKICEAFGANRYPFSED+ +QA MITEVSGR+SELKTTID G
Sbjct: 239  EKNVFVVFYSGERAKSKILKICEAFGANRYPFSEDLGRQAQMITEVSGRLSELKTTIDAG 298

Query: 998  SSQCENLLKTIGEDYERWNILVRREKSIYHTLNMLSIDVTKKCLVAEGWSPISATKKIQD 1177
              Q   LL+TIG+ +E WN+ VR+EK+IYHTLNMLS+DVTKKCLVAEGWSP+ A+++IQD
Sbjct: 299  LGQRNILLQTIGDKFELWNLKVRKEKAIYHTLNMLSLDVTKKCLVAEGWSPVFASREIQD 358

Query: 1178 ALQRATIDSNSQIGGIFRVLHTKESPPTYFKTNKFTSSFQEIVDAYGIAKYQEANPGVYT 1357
            ALQRA +DSNSQ+G IF+VL TKESPPTYF+TNKFTS+ QEIVDAYG+AKYQEANPGV+T
Sbjct: 359  ALQRAAVDSNSQVGSIFQVLRTKESPPTYFRTNKFTSAIQEIVDAYGVAKYQEANPGVFT 418

Query: 1358 IVTFPFLFAVMFGDWGHGICLLLGTLYFIAMEKKFSSQKL 1477
            IVTFPFLFAVMFGDWGHGIC+LL T+Y I  EKK +SQKL
Sbjct: 419  IVTFPFLFAVMFGDWGHGICILLATMYLILKEKKLASQKL 458



 Score =  107 bits (266), Expect(2) = 0.0
 Identities = 50/59 (84%), Positives = 54/59 (91%)
 Frame = +1

Query: 1546 MTFGGRYVILMMSLFSIYTGLIYNEFFSIPFELFSPSAYGCRDPTCGDSTTIGLIKVRD 1722
            M FGGRYVILMMSLFSIYTGLIYNEFFSIPF LF+PSAY CRD +C ++TTIGLIKVRD
Sbjct: 464  MAFGGRYVILMMSLFSIYTGLIYNEFFSIPFPLFAPSAYDCRDVSCSEATTIGLIKVRD 522


>ref|NP_568051.1| V-type H+-transporting ATPase subunit I [Arabidopsis thaliana]
            gi|75162529|sp|Q8W4S4.1|VHAA3_ARATH RecName:
            Full=Vacuolar proton ATPase a3; AltName: Full=V-type
            proton ATPase 95 kDa subunit a isoform 3; Short=V-ATPase
            95 kDa isoform a3; AltName: Full=Vacuolar proton pump
            subunit a3; AltName: Full=Vacuolar proton translocating
            ATPase 95 kDa subunit a isoform 3
            gi|16974583|gb|AAL31187.1| AT4g39080/F19H22_180
            [Arabidopsis thaliana] gi|27363224|gb|AAO11531.1|
            At4g39080/F19H22_180 [Arabidopsis thaliana]
            gi|110742875|dbj|BAE99335.1| hypothetical protein
            [Arabidopsis thaliana] gi|332661615|gb|AEE87015.1| V-type
            H+-transporting ATPase subunit I [Arabidopsis thaliana]
          Length = 821

 Score =  698 bits (1802), Expect(2) = 0.0
 Identities = 343/460 (74%), Positives = 400/460 (86%)
 Frame = +2

Query: 98   MAESGGDSGGCCPPMDLFRSEPMHLVQLILPIESAHLSVSYLGDLGLVQFKDLNSDKSPF 277
            MAESGG  GGCCPPMDL RSE M LVQLI+P+ESAHL+VSYLGDLGLVQFKDLNS+KSPF
Sbjct: 1    MAESGG-GGGCCPPMDLMRSETMQLVQLIVPMESAHLTVSYLGDLGLVQFKDLNSEKSPF 59

Query: 278  QRTYATQIKRCGEMARKLRFFRDQMLKAGVTPPSRSASEDDLDLDTLEVKLGQLEAELIE 457
            QRTYA QIKRCGEMARK+RFFRDQM KAGV        E+D+DLD +EVKLG+LEAEL+E
Sbjct: 60   QRTYAAQIKRCGEMARKIRFFRDQMSKAGVPAKEMQGKENDIDLDDVEVKLGELEAELVE 119

Query: 458  LNANSNKLQRGYNELEEYKLVLQKAGEFFHSALSNAEARQREAGSAQSGEESLETPLLEE 637
            +NAN++KLQR YNEL EYKLVLQKAGEFF SA  +A  +QRE  S Q+GE+ LE+PLL+E
Sbjct: 120  INANNDKLQRSYNELMEYKLVLQKAGEFFSSAHRSAADQQRETESQQAGEDLLESPLLQE 179

Query: 638  QEMTTDQSKQVKLGFISGLVPKVKSLSFERILFRATRGNVYLKQAVVEHPVTDPVSGEKV 817
             E + D +KQVKLGF++GLVP+ KS+ FERILFRATRGN++++Q V+E PV DP SGEK 
Sbjct: 180  -EKSIDSTKQVKLGFLTGLVPREKSMVFERILFRATRGNIFIRQTVIEEPVIDPNSGEKA 238

Query: 818  EKNVFAVFFSGEKAKNKILKICEAFGANRYPFSEDISKQAHMITEVSGRISELKTTIDIG 997
            EKNVF VF+SGE+AK+KILKICEAFGANRYPFSED+ +QA MITEVSGR+SELKTTID G
Sbjct: 239  EKNVFVVFYSGERAKSKILKICEAFGANRYPFSEDLGRQAQMITEVSGRLSELKTTIDAG 298

Query: 998  SSQCENLLKTIGEDYERWNILVRREKSIYHTLNMLSIDVTKKCLVAEGWSPISATKKIQD 1177
              Q   LL+TIG+ +E WN+ VR+EK+IYHTLNMLS+DVTKKCLVAEGWSP+ A+++IQD
Sbjct: 299  LGQRNILLQTIGDKFELWNLKVRKEKAIYHTLNMLSLDVTKKCLVAEGWSPVFASREIQD 358

Query: 1178 ALQRATIDSNSQIGGIFRVLHTKESPPTYFKTNKFTSSFQEIVDAYGIAKYQEANPGVYT 1357
            ALQRA +DSNSQ+G IF+VL TKESPPTYF+TNKFTS+ QEIVDAYG+AKYQEANPGV+T
Sbjct: 359  ALQRAAVDSNSQVGSIFQVLRTKESPPTYFRTNKFTSAIQEIVDAYGVAKYQEANPGVFT 418

Query: 1358 IVTFPFLFAVMFGDWGHGICLLLGTLYFIAMEKKFSSQKL 1477
            IVTFPFLFAVMFGDWGHGIC+LL T+Y I  EKK +SQKL
Sbjct: 419  IVTFPFLFAVMFGDWGHGICILLATMYLILKEKKLASQKL 458



 Score =  107 bits (266), Expect(2) = 0.0
 Identities = 50/59 (84%), Positives = 54/59 (91%)
 Frame = +1

Query: 1546 MTFGGRYVILMMSLFSIYTGLIYNEFFSIPFELFSPSAYGCRDPTCGDSTTIGLIKVRD 1722
            M FGGRYVILMMSLFSIYTGLIYNEFFSIPF LF+PSAY CRD +C ++TTIGLIKVRD
Sbjct: 464  MAFGGRYVILMMSLFSIYTGLIYNEFFSIPFPLFAPSAYDCRDVSCSEATTIGLIKVRD 522


>ref|XP_002866830.1| VHA-A3 [Arabidopsis lyrata subsp. lyrata] gi|297312666|gb|EFH43089.1|
            VHA-A3 [Arabidopsis lyrata subsp. lyrata]
          Length = 820

 Score =  695 bits (1793), Expect(2) = 0.0
 Identities = 342/460 (74%), Positives = 399/460 (86%)
 Frame = +2

Query: 98   MAESGGDSGGCCPPMDLFRSEPMHLVQLILPIESAHLSVSYLGDLGLVQFKDLNSDKSPF 277
            MAESGG  GGCCPPMDL RSE M LVQLI+P+ESAHL+VSYLGDLGLVQFKDLNS+KSPF
Sbjct: 1    MAESGG-GGGCCPPMDLMRSETMQLVQLIVPMESAHLTVSYLGDLGLVQFKDLNSEKSPF 59

Query: 278  QRTYATQIKRCGEMARKLRFFRDQMLKAGVTPPSRSASEDDLDLDTLEVKLGQLEAELIE 457
            QRTYA QIKRCGEMARK+RFF+DQM KAGV        E+D+DLD +EVKLG+LEAEL+E
Sbjct: 60   QRTYAAQIKRCGEMARKIRFFKDQMSKAGVPAKEMLVKENDIDLDDVEVKLGELEAELVE 119

Query: 458  LNANSNKLQRGYNELEEYKLVLQKAGEFFHSALSNAEARQREAGSAQSGEESLETPLLEE 637
            +NAN++KLQR YNEL EYKLVLQKAGEFF SA  +A  +Q E  S Q+GE+ LE+PLL+E
Sbjct: 120  INANNDKLQRSYNELMEYKLVLQKAGEFFSSAHRSATDQQSEIESQQAGEDLLESPLLQE 179

Query: 638  QEMTTDQSKQVKLGFISGLVPKVKSLSFERILFRATRGNVYLKQAVVEHPVTDPVSGEKV 817
             E + D +KQVKLGF++GLVP+ KS+ FERILFRATRGN++++Q V+E PV DP +GEK 
Sbjct: 180  -EKSIDSTKQVKLGFLTGLVPREKSMVFERILFRATRGNIFIRQTVIEEPVIDPNTGEKA 238

Query: 818  EKNVFAVFFSGEKAKNKILKICEAFGANRYPFSEDISKQAHMITEVSGRISELKTTIDIG 997
            EKNVF VF+SGE+AK+KILKICEAFGANRYPFSED+ +QA MITEVSGR+SELKTTID G
Sbjct: 239  EKNVFVVFYSGERAKSKILKICEAFGANRYPFSEDLGRQAQMITEVSGRLSELKTTIDAG 298

Query: 998  SSQCENLLKTIGEDYERWNILVRREKSIYHTLNMLSIDVTKKCLVAEGWSPISATKKIQD 1177
              Q   LL+TIG+ +E WN+ VR+EK+IYHTLNMLS+DVTKKCLVAEGWSP+ A+K+IQD
Sbjct: 299  LGQRNILLQTIGDKFELWNLKVRKEKAIYHTLNMLSLDVTKKCLVAEGWSPVFASKEIQD 358

Query: 1178 ALQRATIDSNSQIGGIFRVLHTKESPPTYFKTNKFTSSFQEIVDAYGIAKYQEANPGVYT 1357
            ALQRA +DSNSQ+G IF+VL TKESPPTYF+TNKFTS+ QEIVDAYG+AKYQEANPGV+T
Sbjct: 359  ALQRAAVDSNSQVGSIFQVLRTKESPPTYFRTNKFTSAIQEIVDAYGVAKYQEANPGVFT 418

Query: 1358 IVTFPFLFAVMFGDWGHGICLLLGTLYFIAMEKKFSSQKL 1477
            IVTFPFLFAVMFGDWGHGIC+LL T+Y I  EKK SSQKL
Sbjct: 419  IVTFPFLFAVMFGDWGHGICILLATMYLILREKKLSSQKL 458



 Score =  106 bits (264), Expect(2) = 0.0
 Identities = 49/59 (83%), Positives = 54/59 (91%)
 Frame = +1

Query: 1546 MTFGGRYVILMMSLFSIYTGLIYNEFFSIPFELFSPSAYGCRDPTCGDSTTIGLIKVRD 1722
            M FGGRYVILMMSLFSIYTGLIYNEFFSIP+ LF+PSAY CRD +C ++TTIGLIKVRD
Sbjct: 464  MAFGGRYVILMMSLFSIYTGLIYNEFFSIPYPLFAPSAYDCRDTSCSEATTIGLIKVRD 522