BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00000140
         (993 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002325535.1| predicted protein [Populus trichocarpa] gi|2...   206   9e-86
emb|CAN78196.1| hypothetical protein VITISV_008800 [Vitis vinifera]   194   8e-85
ref|XP_002264796.2| PREDICTED: carboxymethylenebutenolidase homo...   193   1e-84
ref|XP_002521787.1| hydrolase, putative [Ricinus communis] gi|22...   187   4e-83
ref|XP_003527587.1| PREDICTED: carboxymethylenebutenolidase homo...   176   1e-76

>ref|XP_002325535.1| predicted protein [Populus trichocarpa] gi|222862410|gb|EEE99916.1|
           predicted protein [Populus trichocarpa]
          Length = 294

 Score =  206 bits (523), Expect(2) = 9e-86
 Identities = 95/139 (68%), Positives = 115/139 (82%)
 Frame = +2

Query: 449 KDIDTSIKWMVDEFVAAGSSKKLGVIGFCFGGGRVLDTLARDQXXXXXXXXSFYGTRMDP 628
           KDIDTS KWMVDEF+AAG SKKLG+IGFCFGGGRV+D L+RDQ        SFYGTRM+P
Sbjct: 154 KDIDTSAKWMVDEFLAAGISKKLGIIGFCFGGGRVIDALSRDQGALFGVGVSFYGTRMNP 213

Query: 629 SVAAAVKVPVLFIAGDNDALCPVSGLQEIEKIVAEGSRVIIYEGRGHGFVHRPQSSEEDA 808
           S+A+++KVPVLFI+GDND LC VS L++ EK + +GS+V+I+EGRGHGF HRP S EED 
Sbjct: 214 SLASSIKVPVLFISGDNDPLCTVSVLKDFEKSIGQGSKVVIFEGRGHGFAHRPNSPEEDK 273

Query: 809 DADQAFLEMRNWLHNGLVL 865
           DAD+AF  +R WLH+GLVL
Sbjct: 274 DADEAFTIIRKWLHDGLVL 292



 Score =  138 bits (348), Expect(2) = 9e-86
 Identities = 67/100 (67%), Positives = 82/100 (82%), Gaps = 4/100 (4%)
 Frame = +1

Query: 112 QRLSLSIVKESGSKVLC----STVKIEDTLDDETCELVNGVEISLGEGVDSINAYLFTAV 279
           ++ S+++++       C    S +KIED +DDE CELV+G+EIS+GEG DSI+AYL  A+
Sbjct: 25  RKCSVNLLRSCRGNTACRASSSLLKIEDDIDDEACELVSGLEISIGEGDDSIDAYLLKAM 84

Query: 280 KNNNGTGILLLSDIFGFEDSSTRDFAYRVACNGYNVLLPD 399
           KNNNGTGILLLSDIFGFEDSSTRDFAYRVACNGYNVL+PD
Sbjct: 85  KNNNGTGILLLSDIFGFEDSSTRDFAYRVACNGYNVLIPD 124


>emb|CAN78196.1| hypothetical protein VITISV_008800 [Vitis vinifera]
          Length = 342

 Score =  194 bits (493), Expect(2) = 8e-85
 Identities = 89/146 (60%), Positives = 115/146 (78%)
 Frame = +2

Query: 449 KDIDTSIKWMVDEFVAAGSSKKLGVIGFCFGGGRVLDTLARDQXXXXXXXXSFYGTRMDP 628
           +DID S+KW+VDEF+AAG ++KLGVIGFCFGGGRV+D LA+DQ        SFYGTR++P
Sbjct: 183 RDIDMSVKWLVDEFIAAGVTEKLGVIGFCFGGGRVIDVLAQDQGAHFGTGVSFYGTRINP 242

Query: 629 SVAAAVKVPVLFIAGDNDALCPVSGLQEIEKIVAEGSRVIIYEGRGHGFVHRPQSSEEDA 808
           S AA VKVPVLFI+GD D LCPV+ L++IEK +  GSR++I+EGRGHGF HRP+S E+D 
Sbjct: 243 SAAANVKVPVLFISGDEDPLCPVNLLKDIEKSIGRGSRLVIFEGRGHGFAHRPESPEDDE 302

Query: 809 DADQAFLEMRNWLHNGLVLVQ*MLVY 886
           DA+QAF  +R+WL  GL +    L++
Sbjct: 303 DAEQAFAVLRSWLQEGLAVTSETLLH 328



 Score =  147 bits (370), Expect(2) = 8e-85
 Identities = 69/91 (75%), Positives = 79/91 (86%)
 Frame = +1

Query: 127 SIVKESGSKVLCSTVKIEDTLDDETCELVNGVEISLGEGVDSINAYLFTAVKNNNGTGIL 306
           S+ K +  +V    VK+ED++DDE CELVNGVE+S+GEG DSINAYL  AVKNNNGTGIL
Sbjct: 63  SLTKNTACRVSSGQVKVEDSVDDEACELVNGVELSIGEGDDSINAYLLKAVKNNNGTGIL 122

Query: 307 LLSDIFGFEDSSTRDFAYRVACNGYNVLLPD 399
           LLSD+FGFEDSSTRDFAYRVACNGYNVL+PD
Sbjct: 123 LLSDVFGFEDSSTRDFAYRVACNGYNVLVPD 153


>ref|XP_002264796.2| PREDICTED: carboxymethylenebutenolidase homolog [Vitis vinifera]
           gi|296089525|emb|CBI39344.3| unnamed protein product
           [Vitis vinifera]
          Length = 312

 Score =  193 bits (491), Expect(2) = 1e-84
 Identities = 88/139 (63%), Positives = 112/139 (80%)
 Frame = +2

Query: 449 KDIDTSIKWMVDEFVAAGSSKKLGVIGFCFGGGRVLDTLARDQXXXXXXXXSFYGTRMDP 628
           +DID S+KW+VDEF+AAG ++KLGVIGFCFGGGRV+D LA+DQ        SFYGTR++P
Sbjct: 172 RDIDMSVKWLVDEFIAAGVTEKLGVIGFCFGGGRVIDVLAQDQGAHFGTGVSFYGTRINP 231

Query: 629 SVAAAVKVPVLFIAGDNDALCPVSGLQEIEKIVAEGSRVIIYEGRGHGFVHRPQSSEEDA 808
           S AA VKVPVLFI+GD D LCPV+ L++IEK +  GSR++I+EGRGHGF HRP+S E+D 
Sbjct: 232 SAAANVKVPVLFISGDEDPLCPVNLLKDIEKSIGRGSRLVIFEGRGHGFAHRPESPEDDE 291

Query: 809 DADQAFLEMRNWLHNGLVL 865
           DA+QAF  +R+WL  GL +
Sbjct: 292 DAEQAFAVLRSWLQEGLAV 310



 Score =  147 bits (370), Expect(2) = 1e-84
 Identities = 69/91 (75%), Positives = 79/91 (86%)
 Frame = +1

Query: 127 SIVKESGSKVLCSTVKIEDTLDDETCELVNGVEISLGEGVDSINAYLFTAVKNNNGTGIL 306
           S+ K +  +V    VK+ED++DDE CELVNGVE+S+GEG DSINAYL  AVKNNNGTGIL
Sbjct: 52  SLTKNTACRVSSGQVKVEDSVDDEACELVNGVELSIGEGDDSINAYLLKAVKNNNGTGIL 111

Query: 307 LLSDIFGFEDSSTRDFAYRVACNGYNVLLPD 399
           LLSD+FGFEDSSTRDFAYRVACNGYNVL+PD
Sbjct: 112 LLSDVFGFEDSSTRDFAYRVACNGYNVLVPD 142


>ref|XP_002521787.1| hydrolase, putative [Ricinus communis] gi|223539000|gb|EEF40597.1|
           hydrolase, putative [Ricinus communis]
          Length = 351

 Score =  187 bits (476), Expect(2) = 4e-83
 Identities = 86/135 (63%), Positives = 110/135 (81%)
 Frame = +2

Query: 449 KDIDTSIKWMVDEFVAAGSSKKLGVIGFCFGGGRVLDTLARDQXXXXXXXXSFYGTRMDP 628
           KDI T+ KWM DEF+AAG SKKLG+IGFCFGGGRV++ L+RDQ        SFYGTRMDP
Sbjct: 179 KDIATAAKWMADEFLAAGISKKLGIIGFCFGGGRVIEVLSRDQGACFGIGVSFYGTRMDP 238

Query: 629 SVAAAVKVPVLFIAGDNDALCPVSGLQEIEKIVAEGSRVIIYEGRGHGFVHRPQSSEEDA 808
           S+A+ +KVPVLFI+G++D LC V+ L++IEK + + SRV+I++GRGHGF HRP S EED 
Sbjct: 239 SLASEIKVPVLFISGNSDPLCSVNVLKDIEKDIGQRSRVVIFQGRGHGFAHRPNSPEEDK 298

Query: 809 DADQAFLEMRNWLHN 853
           DA+QAF+ MRNWL++
Sbjct: 299 DAEQAFVIMRNWLYD 313



 Score =  147 bits (372), Expect(2) = 4e-83
 Identities = 67/83 (80%), Positives = 79/83 (95%)
 Frame = +1

Query: 151 KVLCSTVKIEDTLDDETCELVNGVEISLGEGVDSINAYLFTAVKNNNGTGILLLSDIFGF 330
           +V C+ +K+ED ++DE CELVNGVE+SLGEGVD+I+AYLFTAVKNNNGTG+LLLSDIFGF
Sbjct: 67  QVFCNLLKVEDDINDEACELVNGVELSLGEGVDTISAYLFTAVKNNNGTGVLLLSDIFGF 126

Query: 331 EDSSTRDFAYRVACNGYNVLLPD 399
           EDSSTRDFAYR+ACNGYNVL+PD
Sbjct: 127 EDSSTRDFAYRLACNGYNVLVPD 149


>ref|XP_003527587.1| PREDICTED: carboxymethylenebutenolidase homolog [Glycine max]
          Length = 310

 Score =  176 bits (447), Expect(2) = 1e-76
 Identities = 81/139 (58%), Positives = 105/139 (75%)
 Frame = +2

Query: 449 KDIDTSIKWMVDEFVAAGSSKKLGVIGFCFGGGRVLDTLARDQXXXXXXXXSFYGTRMDP 628
           +DI    KW+VDEF+A G SKKLG+IGFCFGGG+VL  LA+DQ        SFYGTR+DP
Sbjct: 172 EDITRWTKWLVDEFMAVGISKKLGIIGFCFGGGQVLKVLAKDQGACFGTGVSFYGTRIDP 231

Query: 629 SVAAAVKVPVLFIAGDNDALCPVSGLQEIEKIVAEGSRVIIYEGRGHGFVHRPQSSEEDA 808
             A+ +K PVLFI GDND LC VS ++ +EK +A GS+V+++ GRGHGF HRP S EED 
Sbjct: 232 LAASDIKAPVLFILGDNDPLCQVSEIENVEKKIASGSKVVLFPGRGHGFAHRPGSPEEDV 291

Query: 809 DADQAFLEMRNWLHNGLVL 865
           DA+QA++ MR+WL++ LV+
Sbjct: 292 DAEQAYVIMRDWLYDRLVV 310



 Score =  137 bits (345), Expect(2) = 1e-76
 Identities = 63/84 (75%), Positives = 74/84 (88%)
 Frame = +1

Query: 148 SKVLCSTVKIEDTLDDETCELVNGVEISLGEGVDSINAYLFTAVKNNNGTGILLLSDIFG 327
           SK+ CS V +ED +DDE CELV+GVE+SL EG ++I AYLF AVKNNNGTG+LLLSD+FG
Sbjct: 58  SKISCSLVNVEDGIDDEACELVSGVELSLEEGDENIRAYLFKAVKNNNGTGLLLLSDVFG 117

Query: 328 FEDSSTRDFAYRVACNGYNVLLPD 399
           FEDS TRDFAYRVACNGYN+L+PD
Sbjct: 118 FEDSFTRDFAYRVACNGYNILVPD 141