BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00000015
         (1833 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ABB52081.1| putative carotenoid cleavage dioxygenase [Daucus ...   838   0.0  
gb|AFJ94677.1| carotenoid cleavage dioxygenase 1 [Vitis vinifera]     794   0.0  
ref|XP_002278750.1| PREDICTED: carotenoid 9,10(9',10')-cleavage ...   796   0.0  
gb|AAX48772.1| 9,10[9',10']carotenoid cleavage dioxygenase [Viti...   794   0.0  
gb|AFJ94680.1| carotenoid cleavage dioxygenase 1 [Vitis vinifera]     792   0.0  

>gb|ABB52081.1| putative carotenoid cleavage dioxygenase [Daucus carota subsp.
            sativus]
          Length = 547

 Score =  838 bits (2166), Expect(2) = 0.0
 Identities = 409/448 (91%), Positives = 421/448 (93%)
 Frame = +2

Query: 347  YHWFDGDGMIHGLRIKDGKATYVSRYVRTSRIKQEETFGGAKFMKIGDLKGLFGLLMVNM 526
            YHWFDGDGMIHGLRIKDGKATYVSRYVRTSR+KQEETFGGAKFMKIGDLKGLFGLLMVNM
Sbjct: 100  YHWFDGDGMIHGLRIKDGKATYVSRYVRTSRLKQEETFGGAKFMKIGDLKGLFGLLMVNM 159

Query: 527  QMLRGKLKVLDMSYGNGTANTAMIYHHGKLLALSEADKPYAIKVLEDGDLQTLGLLDYDK 706
            QMLRGKL VLDMSYGNGTANTAMIYHHG+LLALSEADKPYAIKVLEDGDLQTLGLLDYDK
Sbjct: 160  QMLRGKLNVLDMSYGNGTANTAMIYHHGRLLALSEADKPYAIKVLEDGDLQTLGLLDYDK 219

Query: 707  RLTHSFTAHPKVDPYTGEMFTFGYSHTPPYITYRVISKDGFMNDPVPITLAEPIMMHDFA 886
            RLTHSFTAHPKVDP+TGEMFTFGYSHTPPYITYRVISKDG MNDPVPITLA+PIMMHDFA
Sbjct: 220  RLTHSFTAHPKVDPFTGEMFTFGYSHTPPYITYRVISKDGVMNDPVPITLADPIMMHDFA 279

Query: 887  ITENYAIIMDLPLYFRPKEMVKEKKLIFTFDATKKARFGVLPRYAKNELLIKWFELPNCF 1066
            ITENYAIIMDLPLYF+PKEMVKEKKLIFTFDATKKARFGVLPRYAKNELLIKWFELPNCF
Sbjct: 280  ITENYAIIMDLPLYFKPKEMVKEKKLIFTFDATKKARFGVLPRYAKNELLIKWFELPNCF 339

Query: 1067 IFHNANAWEEGDDIVLITCRLENPDLDMVNGSXXXXXXXXXXXXXXXXXXXKSGLATQKK 1246
            IFHNANAWEEGD++VLITCRLENPDLDMVNGS                   KSGLA+QKK
Sbjct: 340  IFHNANAWEEGDEVVLITCRLENPDLDMVNGSMKEKLENFNNELYEMRFNMKSGLASQKK 399

Query: 1247 LSASAVDFPRINESYTGRKQRYIYGTILDSIAKVTGIVKFDLHKEPETGKSKLEVGGNVQ 1426
            LSASAVDFPRINESYTGRKQRY+Y T LDSIAKVTGIVKFDLH EPE GK KLEVGGN+Q
Sbjct: 400  LSASAVDFPRINESYTGRKQRYVYATSLDSIAKVTGIVKFDLHMEPEIGKKKLEVGGNIQ 459

Query: 1427 GIFDLGVGRFGSEAIFVPRQPGTSSEEDDGYLIHFLHDEKTGKSAANVIDAKTMSPDPVA 1606
            GIFDLGVGRFGSEAIFVPRQPGT+SEEDDGYLIHFLHDE TGKSAAN+IDAKTMSPDPVA
Sbjct: 460  GIFDLGVGRFGSEAIFVPRQPGTTSEEDDGYLIHFLHDESTGKSAANIIDAKTMSPDPVA 519

Query: 1607 VVELPHRVPYGFHAFFVTEEQLQEQAKL 1690
            VVELPHRVPYGFHAFFVTEEQLQEQAKL
Sbjct: 520  VVELPHRVPYGFHAFFVTEEQLQEQAKL 547



 Score =  104 bits (259), Expect(2) = 0.0
 Identities = 54/74 (72%), Positives = 62/74 (83%), Gaps = 1/74 (1%)
 Frame = +1

Query: 10  MGIAKEDKSE-VERIDGGVVVVNPKPNKGLTSKVIDLIESLIVKFMYDSSVPHHWLSGNF 186
           MG+ + +KSE VERI   VV+VNPKP+KGLTSK ID IESLIVK MYD+SVPHHWL+GNF
Sbjct: 1   MGVTEHEKSEQVERIKD-VVIVNPKPSKGLTSKAIDWIESLIVKLMYDASVPHHWLAGNF 59

Query: 187 APVIDETPPCTNLP 228
           APV DETPP  +LP
Sbjct: 60  APV-DETPPARDLP 72


>gb|AFJ94677.1| carotenoid cleavage dioxygenase 1 [Vitis vinifera]
          Length = 546

 Score =  794 bits (2050), Expect(2) = 0.0
 Identities = 377/448 (84%), Positives = 413/448 (92%)
 Frame = +2

Query: 347  YHWFDGDGMIHGLRIKDGKATYVSRYVRTSRIKQEETFGGAKFMKIGDLKGLFGLLMVNM 526
            YHWFDGDGMIHGL IKDGKATYVSRYVRTSR+KQEE FGGAKFM+IGDLKGLFGLLMVNM
Sbjct: 99   YHWFDGDGMIHGLHIKDGKATYVSRYVRTSRLKQEEYFGGAKFMRIGDLKGLFGLLMVNM 158

Query: 527  QMLRGKLKVLDMSYGNGTANTAMIYHHGKLLALSEADKPYAIKVLEDGDLQTLGLLDYDK 706
            QMLR KLK+LD+SYG GT NTA+++HHGKLLALSEADKPY +KVLEDGDLQTLG+LDYDK
Sbjct: 159  QMLRAKLKILDVSYGTGTGNTALVFHHGKLLALSEADKPYVLKVLEDGDLQTLGMLDYDK 218

Query: 707  RLTHSFTAHPKVDPYTGEMFTFGYSHTPPYITYRVISKDGFMNDPVPITLAEPIMMHDFA 886
            RLTHSFTAHPKVDP+TGEMF+FGYSHTPPYITYRVISKDGFM++PVPIT+++PIMMHDFA
Sbjct: 219  RLTHSFTAHPKVDPFTGEMFSFGYSHTPPYITYRVISKDGFMHEPVPITISDPIMMHDFA 278

Query: 887  ITENYAIIMDLPLYFRPKEMVKEKKLIFTFDATKKARFGVLPRYAKNELLIKWFELPNCF 1066
            ITENYAI MDLPLYFRPKEMVKEKKLIFTFDATKKARFGVLPRYAKNEL IKWFELPNCF
Sbjct: 279  ITENYAIFMDLPLYFRPKEMVKEKKLIFTFDATKKARFGVLPRYAKNELHIKWFELPNCF 338

Query: 1067 IFHNANAWEEGDDIVLITCRLENPDLDMVNGSXXXXXXXXXXXXXXXXXXXKSGLATQKK 1246
            IFHNANAWEE D++VLITCRLENPDLD+V G                    K+G+A+Q+K
Sbjct: 339  IFHNANAWEEEDEVVLITCRLENPDLDLVGGDVKEKLENFGNELYEMRFNMKTGIASQRK 398

Query: 1247 LSASAVDFPRINESYTGRKQRYIYGTILDSIAKVTGIVKFDLHKEPETGKSKLEVGGNVQ 1426
            LSAS+VDFPR+NESYTGRKQRY+YGTILDSIAKVTGI+KFDLH EP+TGKSKLEVGGNVQ
Sbjct: 399  LSASSVDFPRVNESYTGRKQRYVYGTILDSIAKVTGIIKFDLHAEPDTGKSKLEVGGNVQ 458

Query: 1427 GIFDLGVGRFGSEAIFVPRQPGTSSEEDDGYLIHFLHDEKTGKSAANVIDAKTMSPDPVA 1606
            GIFDLGVGRFGSEA+FVPR+PG +SEEDDGYLI F+HDEKTGKS  NVIDAKTMSPDP+A
Sbjct: 459  GIFDLGVGRFGSEAVFVPREPGITSEEDDGYLIFFVHDEKTGKSYVNVIDAKTMSPDPIA 518

Query: 1607 VVELPHRVPYGFHAFFVTEEQLQEQAKL 1690
            +VELP+RVPYGFHAFFVTEEQL+EQAKL
Sbjct: 519  IVELPNRVPYGFHAFFVTEEQLKEQAKL 546



 Score = 92.4 bits (228), Expect(2) = 0.0
 Identities = 44/69 (63%), Positives = 52/69 (75%)
 Frame = +1

Query: 22  KEDKSEVERIDGGVVVVNPKPNKGLTSKVIDLIESLIVKFMYDSSVPHHWLSGNFAPVID 201
           K+   + E+   GV VV+PKP+KG TSK +D +E LIVK MYDSS P H+LSGNFAPV D
Sbjct: 3   KKMAEKEEQGGAGVAVVDPKPSKGFTSKAVDWLEKLIVKLMYDSSQPLHYLSGNFAPVRD 62

Query: 202 ETPPCTNLP 228
           ETPPC NLP
Sbjct: 63  ETPPCKNLP 71


>ref|XP_002278750.1| PREDICTED: carotenoid 9,10(9',10')-cleavage dioxygenase 1 [Vitis
            vinifera] gi|297736256|emb|CBI24894.3| unnamed protein
            product [Vitis vinifera] gi|387763762|gb|AFJ94679.1|
            carotenoid cleavage dioxygenase 1 [Vitis vinifera]
            gi|387763766|gb|AFJ94681.1| carotenoid cleavage
            dioxygenase 1 [Vitis vinifera]
          Length = 546

 Score =  796 bits (2055), Expect(2) = 0.0
 Identities = 378/448 (84%), Positives = 413/448 (92%)
 Frame = +2

Query: 347  YHWFDGDGMIHGLRIKDGKATYVSRYVRTSRIKQEETFGGAKFMKIGDLKGLFGLLMVNM 526
            YHWFDGDGMIHGL IKDGKATYVSRYVRTSR+KQEE FGGAKFM+IGDLKGLFGLLMVNM
Sbjct: 99   YHWFDGDGMIHGLHIKDGKATYVSRYVRTSRLKQEEYFGGAKFMRIGDLKGLFGLLMVNM 158

Query: 527  QMLRGKLKVLDMSYGNGTANTAMIYHHGKLLALSEADKPYAIKVLEDGDLQTLGLLDYDK 706
            QMLR KLK+LD+SYG GT NTA++YHHGKLLALSEADKPY +KVLEDGDLQTLG+LDYDK
Sbjct: 159  QMLRAKLKILDVSYGTGTGNTALVYHHGKLLALSEADKPYVLKVLEDGDLQTLGMLDYDK 218

Query: 707  RLTHSFTAHPKVDPYTGEMFTFGYSHTPPYITYRVISKDGFMNDPVPITLAEPIMMHDFA 886
            RLTHSFTAHPKVDP+TGEMF+FGYSHTPPYITYRVISKDGFM++PVPIT+++PIMMHDFA
Sbjct: 219  RLTHSFTAHPKVDPFTGEMFSFGYSHTPPYITYRVISKDGFMHEPVPITISDPIMMHDFA 278

Query: 887  ITENYAIIMDLPLYFRPKEMVKEKKLIFTFDATKKARFGVLPRYAKNELLIKWFELPNCF 1066
            ITENYAI MDLPLYFRPKEMVKEKKLIFTFDATKKARFGVLPRYAKNEL IKWFELPNCF
Sbjct: 279  ITENYAIFMDLPLYFRPKEMVKEKKLIFTFDATKKARFGVLPRYAKNELHIKWFELPNCF 338

Query: 1067 IFHNANAWEEGDDIVLITCRLENPDLDMVNGSXXXXXXXXXXXXXXXXXXXKSGLATQKK 1246
            IFHNANAWEE D++VLITCRLENPDLD+V G                    K+G+A+Q+K
Sbjct: 339  IFHNANAWEEEDEVVLITCRLENPDLDLVGGDVKEKLENFGNELYEMRFNMKTGIASQRK 398

Query: 1247 LSASAVDFPRINESYTGRKQRYIYGTILDSIAKVTGIVKFDLHKEPETGKSKLEVGGNVQ 1426
            LSAS+VDFPR+NESYTGRKQRY+YGTILDSIAKVTGI+KFDLH EP+TGKSKLEVGGNVQ
Sbjct: 399  LSASSVDFPRVNESYTGRKQRYVYGTILDSIAKVTGIIKFDLHAEPDTGKSKLEVGGNVQ 458

Query: 1427 GIFDLGVGRFGSEAIFVPRQPGTSSEEDDGYLIHFLHDEKTGKSAANVIDAKTMSPDPVA 1606
            GIFDLGVGRFGSEA+FVPR+PG +SEEDDGYLI F+HDEKTGKS  NVIDAKTMSPDP+A
Sbjct: 459  GIFDLGVGRFGSEAVFVPREPGITSEEDDGYLIFFIHDEKTGKSYVNVIDAKTMSPDPIA 518

Query: 1607 VVELPHRVPYGFHAFFVTEEQLQEQAKL 1690
            +VELP+RVPYGFHAFFVTEEQL+EQAKL
Sbjct: 519  IVELPNRVPYGFHAFFVTEEQLKEQAKL 546



 Score = 89.7 bits (221), Expect(2) = 0.0
 Identities = 43/69 (62%), Positives = 52/69 (75%)
 Frame = +1

Query: 22  KEDKSEVERIDGGVVVVNPKPNKGLTSKVIDLIESLIVKFMYDSSVPHHWLSGNFAPVID 201
           K+   + E+   GVVVV+P P+KG TSK +D +E LIVK M+DSS P H+LSGNFAPV D
Sbjct: 3   KKMAEKEEQGGAGVVVVDPTPSKGFTSKAVDWLEKLIVKLMHDSSQPLHYLSGNFAPVRD 62

Query: 202 ETPPCTNLP 228
           ETPPC NLP
Sbjct: 63  ETPPCKNLP 71


>gb|AAX48772.1| 9,10[9',10']carotenoid cleavage dioxygenase [Vitis vinifera]
          Length = 542

 Score =  794 bits (2050), Expect(2) = 0.0
 Identities = 377/448 (84%), Positives = 413/448 (92%)
 Frame = +2

Query: 347  YHWFDGDGMIHGLRIKDGKATYVSRYVRTSRIKQEETFGGAKFMKIGDLKGLFGLLMVNM 526
            YHWFDGDGMIHGL IKDGKATYVSRYVRTSR+KQEE FGGAKFM+IGDLKGLFGLLMVNM
Sbjct: 95   YHWFDGDGMIHGLHIKDGKATYVSRYVRTSRLKQEEYFGGAKFMRIGDLKGLFGLLMVNM 154

Query: 527  QMLRGKLKVLDMSYGNGTANTAMIYHHGKLLALSEADKPYAIKVLEDGDLQTLGLLDYDK 706
            QMLR KLK+LD+SYG GT NTA+++HHGKLLALSEADKPY +KVLEDGDLQTLG+LDYDK
Sbjct: 155  QMLRAKLKILDVSYGTGTGNTALVFHHGKLLALSEADKPYVLKVLEDGDLQTLGMLDYDK 214

Query: 707  RLTHSFTAHPKVDPYTGEMFTFGYSHTPPYITYRVISKDGFMNDPVPITLAEPIMMHDFA 886
            RLTHSFTAHPKVDP+TGEMF+FGYSHTPPYITYRVISKDGFM++PVPIT+++PIMMHDFA
Sbjct: 215  RLTHSFTAHPKVDPFTGEMFSFGYSHTPPYITYRVISKDGFMHEPVPITISDPIMMHDFA 274

Query: 887  ITENYAIIMDLPLYFRPKEMVKEKKLIFTFDATKKARFGVLPRYAKNELLIKWFELPNCF 1066
            ITENYAI MDLPLYFRPKEMVKEKKLIFTFDATKKARFGVLPRYAKNEL IKWFELPNCF
Sbjct: 275  ITENYAIFMDLPLYFRPKEMVKEKKLIFTFDATKKARFGVLPRYAKNELHIKWFELPNCF 334

Query: 1067 IFHNANAWEEGDDIVLITCRLENPDLDMVNGSXXXXXXXXXXXXXXXXXXXKSGLATQKK 1246
            IFHNANAWEE D++VLITCRLENPDLD+V G                    K+G+A+Q+K
Sbjct: 335  IFHNANAWEEEDEVVLITCRLENPDLDLVGGDVKEKLENFGNELYEMRFNMKTGIASQRK 394

Query: 1247 LSASAVDFPRINESYTGRKQRYIYGTILDSIAKVTGIVKFDLHKEPETGKSKLEVGGNVQ 1426
            LSAS+VDFPR+NESYTGRKQRY+YGTILDSIAKVTGI+KFDLH EP+TGKSKLEVGGNVQ
Sbjct: 395  LSASSVDFPRVNESYTGRKQRYVYGTILDSIAKVTGIIKFDLHAEPDTGKSKLEVGGNVQ 454

Query: 1427 GIFDLGVGRFGSEAIFVPRQPGTSSEEDDGYLIHFLHDEKTGKSAANVIDAKTMSPDPVA 1606
            GIFDLGVGRFGSEA+FVPR+PG +SEEDDGYLI F+HDEKTGKS  NVIDAKTMSPDP+A
Sbjct: 455  GIFDLGVGRFGSEAVFVPREPGITSEEDDGYLIFFVHDEKTGKSYVNVIDAKTMSPDPIA 514

Query: 1607 VVELPHRVPYGFHAFFVTEEQLQEQAKL 1690
            +VELP+RVPYGFHAFFVTEEQL+EQAKL
Sbjct: 515  IVELPNRVPYGFHAFFVTEEQLKEQAKL 542



 Score = 91.7 bits (226), Expect(2) = 0.0
 Identities = 43/62 (69%), Positives = 50/62 (80%)
 Frame = +1

Query: 43  ERIDGGVVVVNPKPNKGLTSKVIDLIESLIVKFMYDSSVPHHWLSGNFAPVIDETPPCTN 222
           E+   GVVVV+PKP+KG TSK +D +E LIVK M+DSS P H+LSGNFAPV DETPPC N
Sbjct: 6   EQGGAGVVVVDPKPSKGFTSKAVDWLEKLIVKLMHDSSQPLHYLSGNFAPVRDETPPCKN 65

Query: 223 LP 228
           LP
Sbjct: 66  LP 67


>gb|AFJ94680.1| carotenoid cleavage dioxygenase 1 [Vitis vinifera]
          Length = 546

 Score =  792 bits (2046), Expect(2) = 0.0
 Identities = 377/448 (84%), Positives = 412/448 (91%)
 Frame = +2

Query: 347  YHWFDGDGMIHGLRIKDGKATYVSRYVRTSRIKQEETFGGAKFMKIGDLKGLFGLLMVNM 526
            YHWFDGDGMIHGL IKDGKATYVSRYVRTSR+KQEE FGGAKFM+IGDLKGLFGLLMVNM
Sbjct: 99   YHWFDGDGMIHGLHIKDGKATYVSRYVRTSRLKQEEYFGGAKFMRIGDLKGLFGLLMVNM 158

Query: 527  QMLRGKLKVLDMSYGNGTANTAMIYHHGKLLALSEADKPYAIKVLEDGDLQTLGLLDYDK 706
            QMLR KLK+LD+SYG GT NTA++YHHGKLLALSEADKPY +KVLEDGDLQTLG+LDYDK
Sbjct: 159  QMLRAKLKILDVSYGTGTGNTALVYHHGKLLALSEADKPYVLKVLEDGDLQTLGMLDYDK 218

Query: 707  RLTHSFTAHPKVDPYTGEMFTFGYSHTPPYITYRVISKDGFMNDPVPITLAEPIMMHDFA 886
            RLTHSFTAHPKVDP+TGEMF+FGYSHTPPYITYRVISKDGFM++PVPIT+++PIMMHDFA
Sbjct: 219  RLTHSFTAHPKVDPFTGEMFSFGYSHTPPYITYRVISKDGFMHEPVPITISDPIMMHDFA 278

Query: 887  ITENYAIIMDLPLYFRPKEMVKEKKLIFTFDATKKARFGVLPRYAKNELLIKWFELPNCF 1066
            ITENYAI MDLPLYFRPKEMVKEKKLIFTFDATKKARFGVLPRYAKNEL IKWFELPNCF
Sbjct: 279  ITENYAIFMDLPLYFRPKEMVKEKKLIFTFDATKKARFGVLPRYAKNELHIKWFELPNCF 338

Query: 1067 IFHNANAWEEGDDIVLITCRLENPDLDMVNGSXXXXXXXXXXXXXXXXXXXKSGLATQKK 1246
            IFHNANAWEE D++VLITCRLENPDLD+V G                    K+ +A+Q+K
Sbjct: 339  IFHNANAWEEEDEVVLITCRLENPDLDLVGGDVKEKLENFGNELYEMRFNMKTCIASQRK 398

Query: 1247 LSASAVDFPRINESYTGRKQRYIYGTILDSIAKVTGIVKFDLHKEPETGKSKLEVGGNVQ 1426
            LSAS+VDFPR+NESYTGRKQRY+YGTILDSIAKVTGI+KFDLH EP+TGKSKLEVGGNVQ
Sbjct: 399  LSASSVDFPRVNESYTGRKQRYVYGTILDSIAKVTGIIKFDLHAEPDTGKSKLEVGGNVQ 458

Query: 1427 GIFDLGVGRFGSEAIFVPRQPGTSSEEDDGYLIHFLHDEKTGKSAANVIDAKTMSPDPVA 1606
            GIFDLGVGRFGSEA+FVPR+PG +SEEDDGYLI F+HDEKTGKS  NVIDAKTMSPDP+A
Sbjct: 459  GIFDLGVGRFGSEAVFVPREPGITSEEDDGYLIFFIHDEKTGKSYVNVIDAKTMSPDPIA 518

Query: 1607 VVELPHRVPYGFHAFFVTEEQLQEQAKL 1690
            +VELP+RVPYGFHAFFVTEEQL+EQAKL
Sbjct: 519  IVELPNRVPYGFHAFFVTEEQLKEQAKL 546



 Score = 89.7 bits (221), Expect(2) = 0.0
 Identities = 43/69 (62%), Positives = 52/69 (75%)
 Frame = +1

Query: 22  KEDKSEVERIDGGVVVVNPKPNKGLTSKVIDLIESLIVKFMYDSSVPHHWLSGNFAPVID 201
           K+   + E+   GVVVV+P P+KG TSK +D +E LIVK M+DSS P H+LSGNFAPV D
Sbjct: 3   KKMAEKEEQGGAGVVVVDPTPSKGFTSKAVDWLEKLIVKLMHDSSQPLHYLSGNFAPVRD 62

Query: 202 ETPPCTNLP 228
           ETPPC NLP
Sbjct: 63  ETPPCKNLP 71