BLASTX nr result
ID: Atropa21_contig00006418
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Atropa21_contig00006418
(815 letters)
Database: ./nr
37,332,560 sequences; 13,225,080,153 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_004230019.1| PREDICTED: CDPK-related kinase 3-like [Solan... 484 e-134
ref|XP_006339759.1| PREDICTED: CDPK-related kinase 3-like isofor... 481 e-133
ref|XP_002320890.1| calcium-dependent protein kinase [Populus tr... 449 e-124
ref|XP_002529963.1| calcium-dependent protein kinase, putative [... 448 e-124
ref|XP_006444766.1| hypothetical protein CICLE_v10019386mg [Citr... 448 e-123
ref|XP_003626588.1| Calcium dependent protein kinase-like protei... 447 e-123
ref|XP_002302662.1| calcium-dependent protein kinase [Populus tr... 447 e-123
gb|AFK35666.1| unknown [Medicago truncatula] 443 e-122
ref|XP_002266654.1| PREDICTED: CDPK-related protein kinase [Viti... 443 e-122
ref|XP_004494853.1| PREDICTED: CDPK-related kinase 3-like [Cicer... 442 e-122
gb|EXB83843.1| CDPK-related protein kinase [Morus notabilis] 439 e-121
gb|EMJ20065.1| hypothetical protein PRUPE_ppa003103mg [Prunus pe... 437 e-120
gb|ESW07292.1| hypothetical protein PHAVU_010G117600g [Phaseolus... 436 e-120
ref|XP_006846568.1| hypothetical protein AMTR_s00018p00240170 [A... 434 e-119
ref|XP_004308284.1| PREDICTED: CDPK-related kinase 3-like [Fraga... 433 e-119
ref|XP_003528772.1| PREDICTED: CDPK-related kinase 3-like [Glyci... 432 e-119
ref|XP_004166903.1| PREDICTED: LOW QUALITY PROTEIN: CDPK-related... 432 e-119
ref|XP_004140506.1| PREDICTED: CDPK-related protein kinase-like ... 432 e-119
gb|EOX95616.1| CDPK-related kinase 3 isoform 1 [Theobroma cacao] 431 e-118
gb|ADN34224.1| calcium-dependent protein kinase [Cucumis melo su... 428 e-117
>ref|XP_004230019.1| PREDICTED: CDPK-related kinase 3-like [Solanum lycopersicum]
Length = 589
Score = 484 bits (1246), Expect = e-134
Identities = 235/271 (86%), Positives = 252/271 (92%)
Frame = +1
Query: 1 AYYVAPEVLHRSYSLEADIWSIGVITYILLCGSRPFWARTESGIFRAVLRADPNFEDLPW 180
AYYVAPEVLHRSYSLEADIWSIGVITYILLCGSRPFWARTESGIFR+VLR+DPNFEDLPW
Sbjct: 307 AYYVAPEVLHRSYSLEADIWSIGVITYILLCGSRPFWARTESGIFRSVLRSDPNFEDLPW 366
Query: 181 PSVSPEAKDFVKRLLNKDYRKRMTAAQALTHPWLRSESHPIPLDILVYKLVKSYLHATPX 360
PSVSPEAKDFVKRLLNKDYRKRMTAAQALTHPWLRSESHPIPLDI VYKLVKSYLHATP
Sbjct: 367 PSVSPEAKDFVKRLLNKDYRKRMTAAQALTHPWLRSESHPIPLDIFVYKLVKSYLHATPL 426
Query: 361 XXXXXXXXXXXXTEDELVYLRAQFMLLEPSKDGHISLENFRMALLGNATDAMKESRVPDI 540
TEDELVYLRAQFMLLEPS+DG +S+ENFR+ALLGNAT+AM+ESRV DI
Sbjct: 427 KRAALKALSKALTEDELVYLRAQFMLLEPSQDGRVSIENFRLALLGNATEAMRESRVHDI 486
Query: 541 LNAMATLSYRKLDFEEFCAAAISTYQLEALEGWEQIASVAFEHFEREGNRHISVEELARE 720
LNAM LSY+KLDFEEFCAAAISTYQLEALE WEQIA+VAF+HFE+EGNRH+SVEELARE
Sbjct: 487 LNAMTALSYKKLDFEEFCAAAISTYQLEALEEWEQIAAVAFQHFEQEGNRHVSVEELARE 546
Query: 721 LNVGPTAHNILRDWIRSDGKLSLLGYTKFLH 813
LNVGPTAH+ILRDWIR+DGKL++LGYTKFLH
Sbjct: 547 LNVGPTAHSILRDWIRNDGKLNMLGYTKFLH 577
>ref|XP_006339759.1| PREDICTED: CDPK-related kinase 3-like isoform X1 [Solanum tuberosum]
gi|565345351|ref|XP_006339760.1| PREDICTED: CDPK-related
kinase 3-like isoform X2 [Solanum tuberosum]
Length = 589
Score = 481 bits (1239), Expect = e-133
Identities = 234/271 (86%), Positives = 251/271 (92%)
Frame = +1
Query: 1 AYYVAPEVLHRSYSLEADIWSIGVITYILLCGSRPFWARTESGIFRAVLRADPNFEDLPW 180
AYYVAPEVLHRSYSLEADIWSIGVITYILLCGSRPFWARTESGIFR+VLR+DPNFEDLPW
Sbjct: 307 AYYVAPEVLHRSYSLEADIWSIGVITYILLCGSRPFWARTESGIFRSVLRSDPNFEDLPW 366
Query: 181 PSVSPEAKDFVKRLLNKDYRKRMTAAQALTHPWLRSESHPIPLDILVYKLVKSYLHATPX 360
PSVSPEAKDFVKRLLNKDYRKRMTAAQALTHPWLRSESHPIPLDI VYKLVKSYLHATP
Sbjct: 367 PSVSPEAKDFVKRLLNKDYRKRMTAAQALTHPWLRSESHPIPLDIFVYKLVKSYLHATPL 426
Query: 361 XXXXXXXXXXXXTEDELVYLRAQFMLLEPSKDGHISLENFRMALLGNATDAMKESRVPDI 540
TEDELVYLRAQFMLLEPSKDG +S+ENFR+ALLGNAT+AM+ESRV DI
Sbjct: 427 KRAALKALSKALTEDELVYLRAQFMLLEPSKDGRVSIENFRLALLGNATEAMRESRVHDI 486
Query: 541 LNAMATLSYRKLDFEEFCAAAISTYQLEALEGWEQIASVAFEHFEREGNRHISVEELARE 720
LNAM LSY+KLDFEEFCAAAISTYQLEALE WEQIA+VAF+HFE+EGNRH+SVEELARE
Sbjct: 487 LNAMTALSYKKLDFEEFCAAAISTYQLEALEEWEQIAAVAFQHFEQEGNRHVSVEELARE 546
Query: 721 LNVGPTAHNILRDWIRSDGKLSLLGYTKFLH 813
LNVG TAH+ILRDW+R+DGKL++LGYTKFLH
Sbjct: 547 LNVGQTAHSILRDWMRNDGKLNMLGYTKFLH 577
>ref|XP_002320890.1| calcium-dependent protein kinase [Populus trichocarpa]
gi|222861663|gb|EEE99205.1| calcium-dependent protein
kinase [Populus trichocarpa]
Length = 608
Score = 449 bits (1156), Expect = e-124
Identities = 221/272 (81%), Positives = 242/272 (88%), Gaps = 1/272 (0%)
Frame = +1
Query: 1 AYYVAPEVLHRSYSLEADIWSIGVITYILLCGSRPFWARTESGIFRAVLRADPNFEDLPW 180
AYYVAPEVLHRSYSLEADIWSIGVITYIL+CGSRPFWARTESGIFRAVLR+DPNFEDLPW
Sbjct: 325 AYYVAPEVLHRSYSLEADIWSIGVITYILICGSRPFWARTESGIFRAVLRSDPNFEDLPW 384
Query: 181 PSVSPEAKDFVKRLLNKDYRKRMTAAQALTHPWLRSESHPIPLDILVYKLVKSYLHATPX 360
PSV+PEAKDFVKRLLNKDYRKRMTA QALTHPWLR +S PI LDIL+YKLVK+YLHATP
Sbjct: 385 PSVTPEAKDFVKRLLNKDYRKRMTAVQALTHPWLRDDSRPINLDILIYKLVKAYLHATPF 444
Query: 361 XXXXXXXXXXXXTEDELVYLRAQFMLLEPSKDGHISLENFRMALLGNATDAMKESRVPDI 540
TEDELVYLRAQF LLEP+ DG +SL+NFRMAL+ NATDAM+ESR P+I
Sbjct: 445 KRAALKALSKALTEDELVYLRAQFSLLEPNGDGSVSLDNFRMALVRNATDAMRESRAPEI 504
Query: 541 LNAMATLSYRKLDFEEFCAAAISTYQLEALEGWEQIASVAFEHFEREGNRHISVEELARE 720
LNAM +L+YRK+ FEEFCAAAISTYQLEALEGWEQIA AFEHFE+EGNR ISVEELARE
Sbjct: 505 LNAMESLAYRKMYFEEFCAAAISTYQLEALEGWEQIAFTAFEHFEQEGNRVISVEELARE 564
Query: 721 LNVGPTAHNILRDWIR-SDGKLSLLGYTKFLH 813
LNVGP+A+ ++DWIR SDGKLSL+GYTKFLH
Sbjct: 565 LNVGPSAYTFIKDWIRNSDGKLSLIGYTKFLH 596
>ref|XP_002529963.1| calcium-dependent protein kinase, putative [Ricinus communis]
gi|223530525|gb|EEF32406.1| calcium-dependent protein
kinase, putative [Ricinus communis]
Length = 602
Score = 448 bits (1153), Expect = e-124
Identities = 219/272 (80%), Positives = 243/272 (89%), Gaps = 1/272 (0%)
Frame = +1
Query: 1 AYYVAPEVLHRSYSLEADIWSIGVITYILLCGSRPFWARTESGIFRAVLRADPNFEDLPW 180
AYYVAPEVLHRSYSLEADIWSIGVI+YILLCGSRPFWARTESGIFR+VLR+DPNF+DLPW
Sbjct: 319 AYYVAPEVLHRSYSLEADIWSIGVISYILLCGSRPFWARTESGIFRSVLRSDPNFDDLPW 378
Query: 181 PSVSPEAKDFVKRLLNKDYRKRMTAAQALTHPWLRSESHPIPLDILVYKLVKSYLHATPX 360
PSV+PE KDFVKRLLNKDYRKRMTA QALTHPWLR +S IPLD+L+YKLVK YLHATP
Sbjct: 379 PSVTPEGKDFVKRLLNKDYRKRMTAVQALTHPWLRDDSRSIPLDVLIYKLVKIYLHATPF 438
Query: 361 XXXXXXXXXXXXTEDELVYLRAQFMLLEPSKDGHISLENFRMALLGNATDAMKESRVPDI 540
TEDELVYLRAQF LLEP++DG +SL+NF+MAL+ +ATDAM+ESRVP+I
Sbjct: 439 KRAALKALSKALTEDELVYLRAQFRLLEPNRDGSVSLDNFKMALVRHATDAMRESRVPEI 498
Query: 541 LNAMATLSYRKLDFEEFCAAAISTYQLEALEGWEQIASVAFEHFEREGNRHISVEELARE 720
LNAM L+YRK+ FEEFCAAAISTYQLEALEGWEQIAS AFEHFEREGNR ISVEELARE
Sbjct: 499 LNAMEALAYRKMYFEEFCAAAISTYQLEALEGWEQIASTAFEHFEREGNRVISVEELARE 558
Query: 721 LNVGPTAHNILRDWIR-SDGKLSLLGYTKFLH 813
LNVGP+A++ ++DWIR SDGKLSLLGYTKFLH
Sbjct: 559 LNVGPSAYSFIKDWIRNSDGKLSLLGYTKFLH 590
>ref|XP_006444766.1| hypothetical protein CICLE_v10019386mg [Citrus clementina]
gi|568876557|ref|XP_006491344.1| PREDICTED: CDPK-related
kinase 3-like [Citrus sinensis]
gi|557547028|gb|ESR58006.1| hypothetical protein
CICLE_v10019386mg [Citrus clementina]
Length = 601
Score = 448 bits (1152), Expect = e-123
Identities = 220/272 (80%), Positives = 243/272 (89%), Gaps = 1/272 (0%)
Frame = +1
Query: 1 AYYVAPEVLHRSYSLEADIWSIGVITYILLCGSRPFWARTESGIFRAVLRADPNFEDLPW 180
AYYVAPEVLHRSYSLEADIWSIGVI+YILLCGSRPFWARTESGIFRAVLR+DPNF+DLPW
Sbjct: 318 AYYVAPEVLHRSYSLEADIWSIGVISYILLCGSRPFWARTESGIFRAVLRSDPNFDDLPW 377
Query: 181 PSVSPEAKDFVKRLLNKDYRKRMTAAQALTHPWLRSESHPIPLDILVYKLVKSYLHATPX 360
PSVSPEAKDFVKRLLNKDYRKRMTA QALTHPWLR +S P+PLDIL++KLVKSYLHATP
Sbjct: 378 PSVSPEAKDFVKRLLNKDYRKRMTAVQALTHPWLRDDSRPVPLDILIFKLVKSYLHATPF 437
Query: 361 XXXXXXXXXXXXTEDELVYLRAQFMLLEPSKDGHISLENFRMALLGNATDAMKESRVPDI 540
TEDELVYLRAQF LLEP+KDG +SLENFRM L NATDAM+ESRVPD+
Sbjct: 438 KRAALKALSKALTEDELVYLRAQFRLLEPNKDGSVSLENFRMVLGRNATDAMRESRVPDV 497
Query: 541 LNAMATLSYRKLDFEEFCAAAISTYQLEALEGWEQIASVAFEHFEREGNRHISVEELARE 720
L++M +L+YRK+ FEEFCAAAIST+QLEALEGWEQIAS AFE+FE EGNR IS+EELARE
Sbjct: 498 LSSMESLAYRKMYFEEFCAAAISTHQLEALEGWEQIASTAFEYFEEEGNRVISIEELARE 557
Query: 721 LNVGPTAHNILRDWIR-SDGKLSLLGYTKFLH 813
LNVGP+A++ L+DWIR SDGKLSL GYTKFLH
Sbjct: 558 LNVGPSAYSFLKDWIRNSDGKLSLHGYTKFLH 589
>ref|XP_003626588.1| Calcium dependent protein kinase-like protein [Medicago truncatula]
gi|355501603|gb|AES82806.1| Calcium dependent protein
kinase-like protein [Medicago truncatula]
Length = 596
Score = 447 bits (1150), Expect = e-123
Identities = 217/272 (79%), Positives = 241/272 (88%), Gaps = 1/272 (0%)
Frame = +1
Query: 1 AYYVAPEVLHRSYSLEADIWSIGVITYILLCGSRPFWARTESGIFRAVLRADPNFEDLPW 180
AYYVAPEVLHRSYS+EADIWSIGVITYILLCGSRPFWARTESGIFR VLRADPNF+DLPW
Sbjct: 313 AYYVAPEVLHRSYSVEADIWSIGVITYILLCGSRPFWARTESGIFRTVLRADPNFDDLPW 372
Query: 181 PSVSPEAKDFVKRLLNKDYRKRMTAAQALTHPWLRSESHPIPLDILVYKLVKSYLHATPX 360
PSVSPE KDFVKRLLNKDYRKRMTAAQALTHPWLR ES PIPLDIL+YKLVKSYLHATP
Sbjct: 373 PSVSPEGKDFVKRLLNKDYRKRMTAAQALTHPWLRDESRPIPLDILIYKLVKSYLHATPF 432
Query: 361 XXXXXXXXXXXXTEDELVYLRAQFMLLEPSKDGHISLENFRMALLGNATDAMKESRVPDI 540
T+D+LVYLRAQF LLEP++DGH+SL+NF+MAL +ATDAM+ESRV DI
Sbjct: 433 KRAAVKALSKALTDDQLVYLRAQFRLLEPNRDGHVSLDNFKMALARHATDAMRESRVLDI 492
Query: 541 LNAMATLSYRKLDFEEFCAAAISTYQLEALEGWEQIASVAFEHFEREGNRHISVEELARE 720
++ M L+YRK+DFEEFCAAA STYQLEAL+GWE IA AFEHFE EGNR IS+EELARE
Sbjct: 493 IHTMEPLAYRKMDFEEFCAAATSTYQLEALDGWEDIACAAFEHFESEGNRVISIEELARE 552
Query: 721 LNVGPTAHNILRDWIR-SDGKLSLLGYTKFLH 813
LN+GP+A+++LRDWIR +DGKLSLLGYTKFLH
Sbjct: 553 LNLGPSAYSVLRDWIRNTDGKLSLLGYTKFLH 584
>ref|XP_002302662.1| calcium-dependent protein kinase [Populus trichocarpa]
gi|222844388|gb|EEE81935.1| calcium-dependent protein
kinase [Populus trichocarpa]
Length = 604
Score = 447 bits (1149), Expect = e-123
Identities = 221/272 (81%), Positives = 241/272 (88%), Gaps = 1/272 (0%)
Frame = +1
Query: 1 AYYVAPEVLHRSYSLEADIWSIGVITYILLCGSRPFWARTESGIFRAVLRADPNFEDLPW 180
AYYVAPEVLHRSYSLEADIWSIG+ITYILLCGSRPFWARTESGIFRAVLR+DPNFEDLPW
Sbjct: 321 AYYVAPEVLHRSYSLEADIWSIGIITYILLCGSRPFWARTESGIFRAVLRSDPNFEDLPW 380
Query: 181 PSVSPEAKDFVKRLLNKDYRKRMTAAQALTHPWLRSESHPIPLDILVYKLVKSYLHATPX 360
PSV+PEAKDFVKRLLNKDYRKRMTA QALTHPWLR +S PI +DIL+YKLVK YLHATP
Sbjct: 381 PSVTPEAKDFVKRLLNKDYRKRMTAVQALTHPWLRDDSRPIHVDILIYKLVKVYLHATPF 440
Query: 361 XXXXXXXXXXXXTEDELVYLRAQFMLLEPSKDGHISLENFRMALLGNATDAMKESRVPDI 540
TEDEL YLRAQF LL P+ DG +SL+NFRMAL+ NATDAM+ESRVP+I
Sbjct: 441 KRAALKALSKALTEDELFYLRAQFNLLGPNGDGSVSLDNFRMALVHNATDAMRESRVPEI 500
Query: 541 LNAMATLSYRKLDFEEFCAAAISTYQLEALEGWEQIASVAFEHFEREGNRHISVEELARE 720
LNAM +LSYRK+ FEEFCAAAISTYQLEALEGWEQIAS AFEHFE+EGNR ISVEELARE
Sbjct: 501 LNAMESLSYRKMYFEEFCAAAISTYQLEALEGWEQIASTAFEHFEQEGNRVISVEELARE 560
Query: 721 LNVGPTAHNILRDWIR-SDGKLSLLGYTKFLH 813
LNVGP+A+ I++DWIR SDGKLS+LGY KFLH
Sbjct: 561 LNVGPSAYTIIKDWIRSSDGKLSVLGYAKFLH 592
>gb|AFK35666.1| unknown [Medicago truncatula]
Length = 414
Score = 443 bits (1140), Expect = e-122
Identities = 216/272 (79%), Positives = 240/272 (88%), Gaps = 1/272 (0%)
Frame = +1
Query: 1 AYYVAPEVLHRSYSLEADIWSIGVITYILLCGSRPFWARTESGIFRAVLRADPNFEDLPW 180
AYYVAPEVLHRSYS+EADIWSIGVITYILLCGSRPFWARTESGIFR VLRAD NF+DLPW
Sbjct: 131 AYYVAPEVLHRSYSVEADIWSIGVITYILLCGSRPFWARTESGIFRTVLRADLNFDDLPW 190
Query: 181 PSVSPEAKDFVKRLLNKDYRKRMTAAQALTHPWLRSESHPIPLDILVYKLVKSYLHATPX 360
PSVSPE KDFVKRLLNKDYRKRMTAAQALTHPWLR ES PIPLDIL+YKLVKSYLHATP
Sbjct: 191 PSVSPEGKDFVKRLLNKDYRKRMTAAQALTHPWLRDESRPIPLDILIYKLVKSYLHATPF 250
Query: 361 XXXXXXXXXXXXTEDELVYLRAQFMLLEPSKDGHISLENFRMALLGNATDAMKESRVPDI 540
T+D+LVYLRAQF LLEP++DGH+SL+NF+MAL +ATDAM+ESRV DI
Sbjct: 251 KRAAVKALSKALTDDQLVYLRAQFRLLEPNRDGHVSLDNFKMALARHATDAMRESRVLDI 310
Query: 541 LNAMATLSYRKLDFEEFCAAAISTYQLEALEGWEQIASVAFEHFEREGNRHISVEELARE 720
++ M L+YRK+DFEEFCAAA STYQLEAL+GWE IA AFEHFE EGNR IS+EELARE
Sbjct: 311 IHTMEPLAYRKMDFEEFCAAATSTYQLEALDGWEDIACAAFEHFESEGNRVISIEELARE 370
Query: 721 LNVGPTAHNILRDWIR-SDGKLSLLGYTKFLH 813
LN+GP+A+++LRDWIR +DGKLSLLGYTKFLH
Sbjct: 371 LNLGPSAYSVLRDWIRNTDGKLSLLGYTKFLH 402
>ref|XP_002266654.1| PREDICTED: CDPK-related protein kinase [Vitis vinifera]
gi|296088985|emb|CBI38688.3| unnamed protein product
[Vitis vinifera]
Length = 591
Score = 443 bits (1139), Expect = e-122
Identities = 219/272 (80%), Positives = 241/272 (88%), Gaps = 1/272 (0%)
Frame = +1
Query: 1 AYYVAPEVLHRSYSLEADIWSIGVITYILLCGSRPFWARTESGIFRAVLRADPNFEDLPW 180
AYYVAPEVLHRSY LEADIWS+GVITYILLCGSRPFWARTESGIFRAVLRADPNF+DLPW
Sbjct: 308 AYYVAPEVLHRSYYLEADIWSVGVITYILLCGSRPFWARTESGIFRAVLRADPNFDDLPW 367
Query: 181 PSVSPEAKDFVKRLLNKDYRKRMTAAQALTHPWLRSESHPIPLDILVYKLVKSYLHATPX 360
P+VS EAKDFVKRLLNKDYRKRMTA QALTHPWLR SHPIPLDIL+YKLVKSYL ATP
Sbjct: 368 PTVSAEAKDFVKRLLNKDYRKRMTAVQALTHPWLRDGSHPIPLDILIYKLVKSYLLATPF 427
Query: 361 XXXXXXXXXXXXTEDELVYLRAQFMLLEPSKDGHISLENFRMALLGNATDAMKESRVPDI 540
TEDELVYLR+QF LLEP +DG +SL++F+MAL NATDAMKESRVPDI
Sbjct: 428 KRAALKALSKALTEDELVYLRSQFKLLEPGRDGSVSLDSFKMALARNATDAMKESRVPDI 487
Query: 541 LNAMATLSYRKLDFEEFCAAAISTYQLEALEGWEQIASVAFEHFEREGNRHISVEELARE 720
LNA+ L YR++DFEEFCAAAIST+QLEALEGWEQIAS AFE FE+EGNR ISVEELARE
Sbjct: 488 LNALEPLCYRRMDFEEFCAAAISTHQLEALEGWEQIASTAFEIFEQEGNRVISVEELARE 547
Query: 721 LNVGPTAHNILRDWIR-SDGKLSLLGYTKFLH 813
LN+GP+A+++L++WIR SDGKLSLLGYTKFLH
Sbjct: 548 LNLGPSAYSVLKEWIRNSDGKLSLLGYTKFLH 579
>ref|XP_004494853.1| PREDICTED: CDPK-related kinase 3-like [Cicer arietinum]
Length = 593
Score = 442 bits (1136), Expect = e-122
Identities = 216/272 (79%), Positives = 242/272 (88%), Gaps = 1/272 (0%)
Frame = +1
Query: 1 AYYVAPEVLHRSYSLEADIWSIGVITYILLCGSRPFWARTESGIFRAVLRADPNFEDLPW 180
AYYVAPEVLHRSYS+EADIWSIGVITYILLCGSRPFWARTESGIFR VLRADPNF+DLPW
Sbjct: 310 AYYVAPEVLHRSYSVEADIWSIGVITYILLCGSRPFWARTESGIFRTVLRADPNFDDLPW 369
Query: 181 PSVSPEAKDFVKRLLNKDYRKRMTAAQALTHPWLRSESHPIPLDILVYKLVKSYLHATPX 360
PSVSPEAKDFVKRLLNKDYRKRMTAAQALTHPWLR ES PIPLDIL+YKLVKSYLHATP
Sbjct: 370 PSVSPEAKDFVKRLLNKDYRKRMTAAQALTHPWLRDESRPIPLDILIYKLVKSYLHATPF 429
Query: 361 XXXXXXXXXXXXTEDELVYLRAQFMLLEPSKDGHISLENFRMALLGNATDAMKESRVPDI 540
TED+LVYLRAQF LLEP+++GHISL+NF+MAL +ATDAM+ESRV DI
Sbjct: 430 KRAAVKALSKALTEDQLVYLRAQFRLLEPNREGHISLDNFKMALARHATDAMRESRVLDI 489
Query: 541 LNAMATLSYRKLDFEEFCAAAISTYQLEALEGWEQIASVAFEHFEREGNRHISVEELARE 720
++ M L+YRK+DFEEFCAAA STYQLEAL+ WE IA+ AF++FE EGNR IS+EELARE
Sbjct: 490 IHTMEPLAYRKMDFEEFCAAATSTYQLEALDRWEDIATTAFDYFEIEGNRVISIEELARE 549
Query: 721 LNVGPTAHNILRDWIR-SDGKLSLLGYTKFLH 813
LN+GP+A++I+RDWIR +DGKLSLLGYTKFLH
Sbjct: 550 LNLGPSAYSIIRDWIRNTDGKLSLLGYTKFLH 581
>gb|EXB83843.1| CDPK-related protein kinase [Morus notabilis]
Length = 663
Score = 439 bits (1128), Expect = e-121
Identities = 217/272 (79%), Positives = 237/272 (87%), Gaps = 1/272 (0%)
Frame = +1
Query: 1 AYYVAPEVLHRSYSLEADIWSIGVITYILLCGSRPFWARTESGIFRAVLRADPNFEDLPW 180
AYYVAPEVLHRSYSLE DIWSIGVITYILLCGSRPFWARTESGIFRAVLRADPNF+DLPW
Sbjct: 380 AYYVAPEVLHRSYSLEGDIWSIGVITYILLCGSRPFWARTESGIFRAVLRADPNFDDLPW 439
Query: 181 PSVSPEAKDFVKRLLNKDYRKRMTAAQALTHPWLRSESHPIPLDILVYKLVKSYLHATPX 360
PSVS EAKDFVKRLLNKDYRKRMTA QALTHPWLR+++ P+PLDIL+YKLVK+YLHATP
Sbjct: 440 PSVSQEAKDFVKRLLNKDYRKRMTAVQALTHPWLRNDNRPVPLDILIYKLVKTYLHATPF 499
Query: 361 XXXXXXXXXXXXTEDELVYLRAQFMLLEPSKDGHISLENFRMALLGNATDAMKESRVPDI 540
TEDEL+YLRAQF LLEP DG ISL NF MAL NAT+AM+ESRV D+
Sbjct: 500 KRAALKALSKALTEDELIYLRAQFTLLEPDADGRISLHNFTMALEQNATEAMRESRVLDV 559
Query: 541 LNAMATLSYRKLDFEEFCAAAISTYQLEALEGWEQIASVAFEHFEREGNRHISVEELARE 720
LNAM L+YRK+DFEEFCAAAIST+QLEA+E WEQIAS AF HFE EGNR ISVEELARE
Sbjct: 560 LNAMEPLAYRKMDFEEFCAAAISTHQLEAVERWEQIASTAFHHFELEGNRVISVEELARE 619
Query: 721 LNVGPTAHNILRDWIR-SDGKLSLLGYTKFLH 813
LN+GP+ ++ILRDWIR SDGKLSLLGYTK+LH
Sbjct: 620 LNLGPSTYSILRDWIRNSDGKLSLLGYTKYLH 651
>gb|EMJ20065.1| hypothetical protein PRUPE_ppa003103mg [Prunus persica]
Length = 603
Score = 437 bits (1124), Expect = e-120
Identities = 214/272 (78%), Positives = 238/272 (87%), Gaps = 1/272 (0%)
Frame = +1
Query: 1 AYYVAPEVLHRSYSLEADIWSIGVITYILLCGSRPFWARTESGIFRAVLRADPNFEDLPW 180
AYYVAPEVLHRSYSLE DIWSIGVITYILLCGSRPFWARTESGIFRAVLRADP+F+DLPW
Sbjct: 320 AYYVAPEVLHRSYSLEGDIWSIGVITYILLCGSRPFWARTESGIFRAVLRADPSFDDLPW 379
Query: 181 PSVSPEAKDFVKRLLNKDYRKRMTAAQALTHPWLRSESHPIPLDILVYKLVKSYLHATPX 360
PS+S EAKDFV+RLLNKDYRKRMTA QAL HPWLR++S P+PLD+L+YKLVKSYLHATP
Sbjct: 380 PSMSLEAKDFVRRLLNKDYRKRMTAVQALAHPWLRNDSRPLPLDVLIYKLVKSYLHATPF 439
Query: 361 XXXXXXXXXXXXTEDELVYLRAQFMLLEPSKDGHISLENFRMALLGNATDAMKESRVPDI 540
TEDEL+YLRAQFMLLEP D +SL NF+ AL N+T+AM+ESRV DI
Sbjct: 440 KRATLKSLSKALTEDELIYLRAQFMLLEPDNDERVSLLNFKTALQRNSTEAMRESRVVDI 499
Query: 541 LNAMATLSYRKLDFEEFCAAAISTYQLEALEGWEQIASVAFEHFEREGNRHISVEELARE 720
LNAM L+YR +DFEEFCAAAIST+QLEALEGWEQIAS AFEHFEREGNR IS+EELARE
Sbjct: 500 LNAMEPLAYRAMDFEEFCAAAISTHQLEALEGWEQIASTAFEHFEREGNRVISIEELARE 559
Query: 721 LNVGPTAHNILRDWIRS-DGKLSLLGYTKFLH 813
LN+GP+A+++LRDWIRS DGKLS LGYTKFLH
Sbjct: 560 LNLGPSAYSVLRDWIRSADGKLSFLGYTKFLH 591
>gb|ESW07292.1| hypothetical protein PHAVU_010G117600g [Phaseolus vulgaris]
Length = 588
Score = 436 bits (1122), Expect = e-120
Identities = 219/272 (80%), Positives = 237/272 (87%), Gaps = 1/272 (0%)
Frame = +1
Query: 1 AYYVAPEVLHRSYSLEADIWSIGVITYILLCGSRPFWARTESGIFRAVLRADPNFEDLPW 180
AYYVAPEVLHRSYSLEADIWSIGVITYILLCGSRPFWARTESGIFRAVLRADPNF+DLPW
Sbjct: 305 AYYVAPEVLHRSYSLEADIWSIGVITYILLCGSRPFWARTESGIFRAVLRADPNFDDLPW 364
Query: 181 PSVSPEAKDFVKRLLNKDYRKRMTAAQALTHPWLRSESHPIPLDILVYKLVKSYLHATPX 360
PS S EAKDFVKRLLNKDYRKRMTA QAL+HPWLR ES PIPLDILVYKLVK+YL ATP
Sbjct: 365 PSASAEAKDFVKRLLNKDYRKRMTAVQALSHPWLRDESRPIPLDILVYKLVKAYLLATPL 424
Query: 361 XXXXXXXXXXXXTEDELVYLRAQFMLLEPSKDGHISLENFRMALLGNATDAMKESRVPDI 540
EDEL YLRAQF LL P++DG ISL+NF+MAL+ NATDAM+ESRV DI
Sbjct: 425 KRAAVKALSKALPEDELPYLRAQFRLLGPNRDGLISLDNFKMALVHNATDAMRESRVVDI 484
Query: 541 LNAMATLSYRKLDFEEFCAAAISTYQLEALEGWEQIASVAFEHFEREGNRHISVEELARE 720
+NAM L+YRK+DFEEFCAAAISTYQLEAL+ WE IAS AFEHFE EGNR ISVEEL RE
Sbjct: 485 INAMEPLAYRKMDFEEFCAAAISTYQLEALDRWEDIASTAFEHFETEGNRLISVEELGRE 544
Query: 721 LNVGPTAHNILRDWIR-SDGKLSLLGYTKFLH 813
LN+GP+A++IL+DWIR SDGKLSLLGYTKFLH
Sbjct: 545 LNLGPSAYSILKDWIRNSDGKLSLLGYTKFLH 576
>ref|XP_006846568.1| hypothetical protein AMTR_s00018p00240170 [Amborella trichopoda]
gi|548849378|gb|ERN08243.1| hypothetical protein
AMTR_s00018p00240170 [Amborella trichopoda]
Length = 587
Score = 434 bits (1116), Expect = e-119
Identities = 217/272 (79%), Positives = 238/272 (87%), Gaps = 1/272 (0%)
Frame = +1
Query: 1 AYYVAPEVLHRSYSLEADIWSIGVITYILLCGSRPFWARTESGIFRAVLRADPNFEDLPW 180
AYYVAPEVLHRSYS+EADIWSIGVITYILLCGSRPFWARTESGIFRAVLRADPNF+D PW
Sbjct: 304 AYYVAPEVLHRSYSMEADIWSIGVITYILLCGSRPFWARTESGIFRAVLRADPNFDDAPW 363
Query: 181 PSVSPEAKDFVKRLLNKDYRKRMTAAQALTHPWLRSESHPIPLDILVYKLVKSYLHATPX 360
P+VSPEAKDFVKRLL KD+RKRMTAAQALTHPWLR E H IPLDIL+YKLVKSYL ATP
Sbjct: 364 PAVSPEAKDFVKRLLIKDHRKRMTAAQALTHPWLRDEQHHIPLDILIYKLVKSYLRATPF 423
Query: 361 XXXXXXXXXXXXTEDELVYLRAQFMLLEPSKDGHISLENFRMALLGNATDAMKESRVPDI 540
+EDEL+YL +QF LLEP++DG +SLENF+MALL NATDAMKESRV DI
Sbjct: 424 KRAALKALSKSLSEDELLYLSSQFKLLEPNRDGRVSLENFKMALLRNATDAMKESRVLDI 483
Query: 541 LNAMATLSYRKLDFEEFCAAAISTYQLEALEGWEQIASVAFEHFEREGNRHISVEELARE 720
LN+M +LS RK++FEEFCAAAISTYQLEALEGWEQIAS AFE FE EGNR ISVEELA E
Sbjct: 484 LNSMESLSSRKMNFEEFCAAAISTYQLEALEGWEQIASKAFECFEVEGNRVISVEELAGE 543
Query: 721 LNVGPTAHNILRDWIR-SDGKLSLLGYTKFLH 813
+N+GP+AH++L DWIR SDGKLS LGYTKFLH
Sbjct: 544 MNLGPSAHSVLHDWIRHSDGKLSFLGYTKFLH 575
>ref|XP_004308284.1| PREDICTED: CDPK-related kinase 3-like [Fragaria vesca subsp. vesca]
Length = 608
Score = 433 bits (1113), Expect = e-119
Identities = 215/272 (79%), Positives = 237/272 (87%), Gaps = 1/272 (0%)
Frame = +1
Query: 1 AYYVAPEVLHRSYSLEADIWSIGVITYILLCGSRPFWARTESGIFRAVLRADPNFEDLPW 180
AYYVAPEVLHRSYSLE DIWSIGVITYILLCGSRPFWARTESGIFRAVLRADP+F+D PW
Sbjct: 325 AYYVAPEVLHRSYSLEGDIWSIGVITYILLCGSRPFWARTESGIFRAVLRADPSFDDPPW 384
Query: 181 PSVSPEAKDFVKRLLNKDYRKRMTAAQALTHPWLRSESHPIPLDILVYKLVKSYLHATPX 360
PS+S EAKDFVKRLLNKDYRKRMTA QAL+HPWLR +S P+PLDIL+YKLVKSYLHATP
Sbjct: 385 PSMSHEAKDFVKRLLNKDYRKRMTAVQALSHPWLRDDSQPLPLDILIYKLVKSYLHATPF 444
Query: 361 XXXXXXXXXXXXTEDELVYLRAQFMLLEPSKDGHISLENFRMALLGNATDAMKESRVPDI 540
TEDEL+YLRAQFMLL P KD ISL NF+ AL N+T+AM ES+V DI
Sbjct: 445 KRAALKSLSKALTEDELIYLRAQFMLLGPDKDQRISLINFKAALQRNSTEAMMESKVVDI 504
Query: 541 LNAMATLSYRKLDFEEFCAAAISTYQLEALEGWEQIASVAFEHFEREGNRHISVEELARE 720
LNAM L+YRK+DFEEFCAAAIST+QLEALEGWEQIAS AFEHFEREGNR IS+EELARE
Sbjct: 505 LNAMEPLAYRKMDFEEFCAAAISTHQLEALEGWEQIASTAFEHFEREGNRVISIEELARE 564
Query: 721 LNVGPTAHNILRDWIR-SDGKLSLLGYTKFLH 813
LN+G +A++ILRDWIR +DGKLSL+GYTKFLH
Sbjct: 565 LNLGTSAYSILRDWIRNTDGKLSLMGYTKFLH 596
>ref|XP_003528772.1| PREDICTED: CDPK-related kinase 3-like [Glycine max]
Length = 592
Score = 432 bits (1112), Expect = e-119
Identities = 213/272 (78%), Positives = 239/272 (87%), Gaps = 1/272 (0%)
Frame = +1
Query: 1 AYYVAPEVLHRSYSLEADIWSIGVITYILLCGSRPFWARTESGIFRAVLRADPNFEDLPW 180
AYYVAPEVLHRSYSLEADIWSIGVITYILLCGSRPF+ARTESGIFRAVLRADPNF+DLPW
Sbjct: 308 AYYVAPEVLHRSYSLEADIWSIGVITYILLCGSRPFYARTESGIFRAVLRADPNFDDLPW 367
Query: 181 PSVSPEAKDFVKRLLNKDYRKRMTAAQALTHPWLRSESHPIPLDILVYKLVKSYLHATPX 360
P+ S EAKDFVKRLLNKDYRKRMTA QALTHPWLR +S PIPLDILV+KLVK+YLHATP
Sbjct: 368 PTASAEAKDFVKRLLNKDYRKRMTAVQALTHPWLRDDSRPIPLDILVFKLVKAYLHATPF 427
Query: 361 XXXXXXXXXXXXTEDELVYLRAQFMLLEPSKDGHISLENFRMALLGNATDAMKESRVPDI 540
ED+L YL AQF LLEP++DGHISL+NF+MAL+ NATDAM+ESRV +I
Sbjct: 428 KRAAVKALSKALPEDQLPYLSAQFRLLEPNRDGHISLDNFKMALVRNATDAMRESRVLEI 487
Query: 541 LNAMATLSYRKLDFEEFCAAAISTYQLEALEGWEQIASVAFEHFEREGNRHISVEELARE 720
+NAM L+YRK+DFEEFCAA IST+QLEA + WE IAS AFEHFEREGNR ISVEELARE
Sbjct: 488 INAMEPLAYRKMDFEEFCAATISTHQLEAHDRWEDIASTAFEHFEREGNRLISVEELARE 547
Query: 721 LNVGPTAHNILRDWIR-SDGKLSLLGYTKFLH 813
LN+GP+A+++L+DWIR +DGKLSLLGYTKFLH
Sbjct: 548 LNLGPSAYSVLKDWIRNTDGKLSLLGYTKFLH 579
>ref|XP_004166903.1| PREDICTED: LOW QUALITY PROTEIN: CDPK-related protein kinase-like
[Cucumis sativus]
Length = 609
Score = 432 bits (1110), Expect = e-119
Identities = 209/271 (77%), Positives = 237/271 (87%)
Frame = +1
Query: 1 AYYVAPEVLHRSYSLEADIWSIGVITYILLCGSRPFWARTESGIFRAVLRADPNFEDLPW 180
AYYVAPEVLHRSY+LEADIWSIGVITYILLCGSRPFWARTESGIFRAVLRADPNF+DLPW
Sbjct: 327 AYYVAPEVLHRSYTLEADIWSIGVITYILLCGSRPFWARTESGIFRAVLRADPNFDDLPW 386
Query: 181 PSVSPEAKDFVKRLLNKDYRKRMTAAQALTHPWLRSESHPIPLDILVYKLVKSYLHATPX 360
PSVSPEAKDFVKRLLNKDYRKRMTA QALTHPWL+ +S IPLDIL+YKLVKSYL ATP
Sbjct: 387 PSVSPEAKDFVKRLLNKDYRKRMTAVQALTHPWLQDDSRRIPLDILIYKLVKSYLQATPF 446
Query: 361 XXXXXXXXXXXXTEDELVYLRAQFMLLEPSKDGHISLENFRMALLGNATDAMKESRVPDI 540
+E EL YLRAQF LLEP+ DG + L+NF+MAL+ NATDAM+ESRV +I
Sbjct: 447 KRAAMKALSKALSETELFYLRAQFALLEPNHDGRVGLDNFKMALMRNATDAMRESRVHEI 506
Query: 541 LNAMATLSYRKLDFEEFCAAAISTYQLEALEGWEQIASVAFEHFEREGNRHISVEELARE 720
+N++ L+YR++DFEEFCAA+IST+QLEAL+ WEQIA VAFEHFEREGNR ISVEELA+E
Sbjct: 507 VNSLEPLAYRRMDFEEFCAASISTHQLEALDRWEQIACVAFEHFEREGNRVISVEELAKE 566
Query: 721 LNVGPTAHNILRDWIRSDGKLSLLGYTKFLH 813
LN+G +AH+IL++WIR DGKLS LGYTKFLH
Sbjct: 567 LNLGSSAHSILKEWIRGDGKLSFLGYTKFLH 597
>ref|XP_004140506.1| PREDICTED: CDPK-related protein kinase-like [Cucumis sativus]
Length = 609
Score = 432 bits (1110), Expect = e-119
Identities = 209/271 (77%), Positives = 237/271 (87%)
Frame = +1
Query: 1 AYYVAPEVLHRSYSLEADIWSIGVITYILLCGSRPFWARTESGIFRAVLRADPNFEDLPW 180
AYYVAPEVLHRSY+LEADIWSIGVITYILLCGSRPFWARTESGIFRAVLRADPNF+DLPW
Sbjct: 327 AYYVAPEVLHRSYTLEADIWSIGVITYILLCGSRPFWARTESGIFRAVLRADPNFDDLPW 386
Query: 181 PSVSPEAKDFVKRLLNKDYRKRMTAAQALTHPWLRSESHPIPLDILVYKLVKSYLHATPX 360
PSVSPEAKDFVKRLLNKDYRKRMTA QALTHPWL+ +S IPLDIL+YKLVKSYL ATP
Sbjct: 387 PSVSPEAKDFVKRLLNKDYRKRMTAVQALTHPWLQDDSRRIPLDILIYKLVKSYLQATPF 446
Query: 361 XXXXXXXXXXXXTEDELVYLRAQFMLLEPSKDGHISLENFRMALLGNATDAMKESRVPDI 540
+E EL YLRAQF LLEP+ DG + L+NF+MAL+ NATDAM+ESRV +I
Sbjct: 447 KRAAMKALSKALSETELFYLRAQFALLEPNHDGRVGLDNFKMALMRNATDAMRESRVHEI 506
Query: 541 LNAMATLSYRKLDFEEFCAAAISTYQLEALEGWEQIASVAFEHFEREGNRHISVEELARE 720
+N++ L+YR++DFEEFCAA+IST+QLEAL+ WEQIA VAFEHFEREGNR ISVEELA+E
Sbjct: 507 VNSLEPLAYRRMDFEEFCAASISTHQLEALDRWEQIACVAFEHFEREGNRVISVEELAKE 566
Query: 721 LNVGPTAHNILRDWIRSDGKLSLLGYTKFLH 813
LN+G +AH+IL++WIR DGKLS LGYTKFLH
Sbjct: 567 LNLGSSAHSILKEWIRGDGKLSFLGYTKFLH 597
>gb|EOX95616.1| CDPK-related kinase 3 isoform 1 [Theobroma cacao]
Length = 630
Score = 431 bits (1108), Expect = e-118
Identities = 219/296 (73%), Positives = 245/296 (82%), Gaps = 25/296 (8%)
Frame = +1
Query: 1 AYYVAPEVLHRSYSLEADIWSIGVITYILLCGSRPFWARTESGIFRAVLRADPNFEDLPW 180
AYYVAPEVLHRSYSLEADIWSIGVITYILLCGSRPFWARTESGIFR+VLR+DPNF+DLPW
Sbjct: 323 AYYVAPEVLHRSYSLEADIWSIGVITYILLCGSRPFWARTESGIFRSVLRSDPNFDDLPW 382
Query: 181 PSVSPEAKDFVKRLLNKDYRKRMTAAQALT-------HPWLRSESHPIPLDILVYKLVKS 339
PSVSPEAKDFVKRLLNKDYRKRMTA QAL+ HPWL+ +S IPLDIL+YKLVKS
Sbjct: 383 PSVSPEAKDFVKRLLNKDYRKRMTAVQALSKFGVITYHPWLQDDSRTIPLDILIYKLVKS 442
Query: 340 YLHATPXXXXXXXXXXXXXTEDELVYLRAQFMLLEPSKDGHISLENF------------- 480
YLHATP TEDELVYLRAQF LLEP++DG +SLENF
Sbjct: 443 YLHATPFKRVALKALSKALTEDELVYLRAQFRLLEPNRDGSVSLENFKMVIDRLYDPINL 502
Query: 481 ----RMALLGNATDAMKESRVPDILNAMATLSYRKLDFEEFCAAAISTYQLEALEGWEQI 648
+MAL+ NAT+AM ESRVPDI+N M +L+YRK+ FEEFCAAAIST+QLEA+EGWEQI
Sbjct: 503 SLKSKMALVRNATEAMGESRVPDIVNTMGSLAYRKMYFEEFCAAAISTHQLEAVEGWEQI 562
Query: 649 ASVAFEHFEREGNRHISVEELARELNVGPTAHNILRDWIR-SDGKLSLLGYTKFLH 813
AS+AFEHFE+EGNR IS+EELARELNVGP+A++ L+DWIR SDGKLSLLGYTKFLH
Sbjct: 563 ASIAFEHFEQEGNRVISIEELARELNVGPSAYSFLKDWIRISDGKLSLLGYTKFLH 618
>gb|ADN34224.1| calcium-dependent protein kinase [Cucumis melo subsp. melo]
Length = 292
Score = 428 bits (1100), Expect = e-117
Identities = 208/271 (76%), Positives = 236/271 (87%)
Frame = +1
Query: 1 AYYVAPEVLHRSYSLEADIWSIGVITYILLCGSRPFWARTESGIFRAVLRADPNFEDLPW 180
AYYVAPEVLHRSY+LEADIWSIGVITYILLCGSRPFWARTESGIFRAVLRADPNF+DLPW
Sbjct: 10 AYYVAPEVLHRSYTLEADIWSIGVITYILLCGSRPFWARTESGIFRAVLRADPNFDDLPW 69
Query: 181 PSVSPEAKDFVKRLLNKDYRKRMTAAQALTHPWLRSESHPIPLDILVYKLVKSYLHATPX 360
PSVSPEAKDFVKRLLNKDYRKRMTA QALTHPWL+ +S IPLDIL+YKLVKSYL ATP
Sbjct: 70 PSVSPEAKDFVKRLLNKDYRKRMTAVQALTHPWLQDDSRRIPLDILIYKLVKSYLQATPF 129
Query: 361 XXXXXXXXXXXXTEDELVYLRAQFMLLEPSKDGHISLENFRMALLGNATDAMKESRVPDI 540
+E EL YLRAQF LLEP+ DG + L+NF+MAL+ NATDAM+ESRV +I
Sbjct: 130 KRAALKALSKALSETELFYLRAQFALLEPNHDGRVGLDNFKMALMRNATDAMRESRVHEI 189
Query: 541 LNAMATLSYRKLDFEEFCAAAISTYQLEALEGWEQIASVAFEHFEREGNRHISVEELARE 720
+N++ L+YR++DFEEFCAA+IST+QLEAL+ WEQIA VAFEHFE EGNR ISVEELARE
Sbjct: 190 VNSLEPLAYRRMDFEEFCAASISTHQLEALDRWEQIACVAFEHFEGEGNRVISVEELARE 249
Query: 721 LNVGPTAHNILRDWIRSDGKLSLLGYTKFLH 813
LN+G +A++IL++WIR DGKLS LGYTKFLH
Sbjct: 250 LNLGSSAYSILKEWIRGDGKLSFLGYTKFLH 280