BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atropa21_contig00003374
         (930 letters)

Database: ./nr 
           37,332,560 sequences; 13,225,080,153 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_004240841.1| PREDICTED: hydroxyacylglutathione hydrolase ...   496   e-138
ref|XP_006357301.1| PREDICTED: hydroxyacylglutathione hydrolase ...   494   e-137
gb|ABB29955.1| hydroxyacylglutathione hydrolase cytoplasmic-like...   493   e-137
ref|NP_001275099.1| uncharacterized protein LOC102584437 [Solanu...   456   e-126
gb|EMJ19420.1| hypothetical protein PRUPE_ppa007897mg [Prunus pe...   441   e-121
gb|EXC21105.1| Hydroxyacylglutathione hydrolase cytoplasmic [Mor...   438   e-120
ref|XP_002329233.1| predicted protein [Populus trichocarpa] gi|5...   434   e-119
ref|XP_004307750.1| PREDICTED: hydroxyacylglutathione hydrolase ...   432   e-119
gb|EOY20210.1| Metallo-hydrolase/oxidoreductase superfamily prot...   429   e-117
gb|AFK42856.1| unknown [Lotus japonicus]                              424   e-116
ref|XP_002267471.1| PREDICTED: hydroxyacylglutathione hydrolase ...   423   e-116
ref|XP_004146429.1| PREDICTED: hydroxyacylglutathione hydrolase ...   421   e-115
ref|XP_006486126.1| PREDICTED: hydroxyacylglutathione hydrolase ...   420   e-115
ref|XP_006435967.1| hypothetical protein CICLE_v10032268mg [Citr...   420   e-115
ref|XP_004490514.1| PREDICTED: hydroxyacylglutathione hydrolase ...   419   e-114
ref|XP_006595529.1| PREDICTED: uncharacterized protein LOC100527...   418   e-114
gb|AGV54469.1| hydroxyacylglutathione hydrolase [Phaseolus vulga...   417   e-114
ref|XP_003615463.1| Hydroxyacylglutathione hydrolase [Medicago t...   413   e-113
ref|NP_187696.1| hydroxyacylglutathione hydrolase [Arabidopsis t...   412   e-113
ref|XP_002884816.1| glyoxalase 2-2 [Arabidopsis lyrata subsp. ly...   410   e-112

>ref|XP_004240841.1| PREDICTED: hydroxyacylglutathione hydrolase cytoplasmic-like
           [Solanum lycopersicum]
          Length = 258

 Score =  496 bits (1277), Expect = e-138
 Identities = 237/248 (95%), Positives = 244/248 (98%)
 Frame = -3

Query: 928 DNYSYMIIDENTKEAAVVDPVEPHKVLRVAQENEVNLKFVLTTHHHWDHAGGNDKIKQLV 749
           DNYSYMIIDE TKEAAVVDPVEPHKVLRVAQENEV+LKFVLTTHHHWDHAGGNDKIKQL+
Sbjct: 11  DNYSYMIIDETTKEAAVVDPVEPHKVLRVAQENEVDLKFVLTTHHHWDHAGGNDKIKQLI 70

Query: 748 PGIKIYGGSVDNVRGCTDKVENGDKVSLGADISILSLHTPAHTKGHISYYITDKEGEDPA 569
           PGIKIYGGSVDNVRGCTDKVENGD+VSLGADISILSLHTPAHTKGHISYY+TDKEGED A
Sbjct: 71  PGIKIYGGSVDNVRGCTDKVENGDRVSLGADISILSLHTPAHTKGHISYYVTDKEGEDLA 130

Query: 568 VFTGDTLFIAGCGKFFEGTAEQMYQSLCVTLGSLPNPTRVFCGHEYTVKNLQFALTVEPD 389
           VFTGDTLFIAGCGKFFEGTAEQMYQSLCVTLGSLP PTRV+CGHEYTVKNLQFALTVEPD
Sbjct: 131 VFTGDTLFIAGCGKFFEGTAEQMYQSLCVTLGSLPKPTRVYCGHEYTVKNLQFALTVEPD 190

Query: 388 NAKIAQKLAWAEHQRRVGLPTIPSTIQEEFESNPFMRVDLPEVQDKVGCKSPVEALREIR 209
           NAKIA+KLAWAE QRRVGLPTIPSTIQEEFESNPFMRVDLPEVQDKVGCKSPVEA+REIR
Sbjct: 191 NAKIAEKLAWAERQRRVGLPTIPSTIQEEFESNPFMRVDLPEVQDKVGCKSPVEAMREIR 250

Query: 208 QRKDNWKG 185
           QRKDNWKG
Sbjct: 251 QRKDNWKG 258


>ref|XP_006357301.1| PREDICTED: hydroxyacylglutathione hydrolase cytoplasmic-like
           [Solanum tuberosum] gi|76573347|gb|ABA46778.1| unknown
           [Solanum tuberosum]
          Length = 258

 Score =  494 bits (1273), Expect = e-137
 Identities = 236/248 (95%), Positives = 242/248 (97%)
 Frame = -3

Query: 928 DNYSYMIIDENTKEAAVVDPVEPHKVLRVAQENEVNLKFVLTTHHHWDHAGGNDKIKQLV 749
           DNYSYMIIDE TKEAAVVDPVEPHKVLRVAQENEV+LKFVLTTHHHWDHAGGNDKIKQLV
Sbjct: 11  DNYSYMIIDETTKEAAVVDPVEPHKVLRVAQENEVDLKFVLTTHHHWDHAGGNDKIKQLV 70

Query: 748 PGIKIYGGSVDNVRGCTDKVENGDKVSLGADISILSLHTPAHTKGHISYYITDKEGEDPA 569
           PGIKIYGGSVDNVRGCTDKVENGD++SLG DISILSLHTPAHTKGHISYY+TDKEGEDPA
Sbjct: 71  PGIKIYGGSVDNVRGCTDKVENGDRISLGVDISILSLHTPAHTKGHISYYVTDKEGEDPA 130

Query: 568 VFTGDTLFIAGCGKFFEGTAEQMYQSLCVTLGSLPNPTRVFCGHEYTVKNLQFALTVEPD 389
           VFTGDTLFIAGCGKFFEGTAEQMYQSLCVTLGSLP PT V+CGHEYTVKNLQFALTVEPD
Sbjct: 131 VFTGDTLFIAGCGKFFEGTAEQMYQSLCVTLGSLPKPTLVYCGHEYTVKNLQFALTVEPD 190

Query: 388 NAKIAQKLAWAEHQRRVGLPTIPSTIQEEFESNPFMRVDLPEVQDKVGCKSPVEALREIR 209
           NAKIAQKLAWAE QRRVGLPTIPSTIQEE ESNPFMRVDLPEVQDKVGCKSPVEA+REIR
Sbjct: 191 NAKIAQKLAWAERQRRVGLPTIPSTIQEELESNPFMRVDLPEVQDKVGCKSPVEAMREIR 250

Query: 208 QRKDNWKG 185
           QRKDNWKG
Sbjct: 251 QRKDNWKG 258


>gb|ABB29955.1| hydroxyacylglutathione hydrolase cytoplasmic-like [Solanum
           tuberosum]
          Length = 258

 Score =  493 bits (1269), Expect = e-137
 Identities = 235/248 (94%), Positives = 242/248 (97%)
 Frame = -3

Query: 928 DNYSYMIIDENTKEAAVVDPVEPHKVLRVAQENEVNLKFVLTTHHHWDHAGGNDKIKQLV 749
           DNYSYMIIDE TKEAAVVDPVEPHKVLRVAQENEV+LKFVLTTHHHWDHAGGNDKIKQLV
Sbjct: 11  DNYSYMIIDETTKEAAVVDPVEPHKVLRVAQENEVDLKFVLTTHHHWDHAGGNDKIKQLV 70

Query: 748 PGIKIYGGSVDNVRGCTDKVENGDKVSLGADISILSLHTPAHTKGHISYYITDKEGEDPA 569
           PGIKIYGGSVDNVRGCTDKVENGD++SLG DISILSLHTPAHTKGHISYY+TDKEGEDPA
Sbjct: 71  PGIKIYGGSVDNVRGCTDKVENGDRISLGVDISILSLHTPAHTKGHISYYVTDKEGEDPA 130

Query: 568 VFTGDTLFIAGCGKFFEGTAEQMYQSLCVTLGSLPNPTRVFCGHEYTVKNLQFALTVEPD 389
           VFTGDTLFIAGCGKFFEGTAEQMYQSLCVTLGSLP PT V+CGHEYTVKNLQFALTVEPD
Sbjct: 131 VFTGDTLFIAGCGKFFEGTAEQMYQSLCVTLGSLPKPTLVYCGHEYTVKNLQFALTVEPD 190

Query: 388 NAKIAQKLAWAEHQRRVGLPTIPSTIQEEFESNPFMRVDLPEVQDKVGCKSPVEALREIR 209
           NAKIAQKLAWAE QRRVGLPTIPSTIQEE ESNPFMRVDLPEVQDKVGCKSPVEA+REIR
Sbjct: 191 NAKIAQKLAWAERQRRVGLPTIPSTIQEELESNPFMRVDLPEVQDKVGCKSPVEAMREIR 250

Query: 208 QRKDNWKG 185
           QRKDNW+G
Sbjct: 251 QRKDNWQG 258


>ref|NP_001275099.1| uncharacterized protein LOC102584437 [Solanum tuberosum]
           gi|413968450|gb|AFW90562.1| hydroxyacylglutathione
           hydrolase [Solanum tuberosum]
          Length = 257

 Score =  456 bits (1173), Expect = e-126
 Identities = 223/248 (89%), Positives = 230/248 (92%)
 Frame = -3

Query: 928 DNYSYMIIDENTKEAAVVDPVEPHKVLRVAQENEVNLKFVLTTHHHWDHAGGNDKIKQLV 749
           DNYSYMIIDE TKEAAVVDPVEPHKVLRVAQENEV+LKFVLTTHHHWDHAGGNDKIKQLV
Sbjct: 11  DNYSYMIIDETTKEAAVVDPVEPHKVLRVAQENEVDLKFVLTTHHHWDHAGGNDKIKQLV 70

Query: 748 PGIKIYGGSVDNVRGCTDKVENGDKVSLGADISILSLHTPAHTKGHISYYITDKEGEDPA 569
           PGIKIYGGSVDNVRGCTDKVENGD+VSLG DISILSLHTPAHTKGHISYY+TDKEGEDPA
Sbjct: 71  PGIKIYGGSVDNVRGCTDKVENGDRVSLGVDISILSLHTPAHTKGHISYYVTDKEGEDPA 130

Query: 568 VFTGDTLFIAGCGKFFEGTAEQMYQSLCVTLGSLPNPTRVFCGHEYTVKNLQFALTVEPD 389
           VFTGDTLFIAGCGKFFEGTAEQMYQSLCVTLGSLP PT V+CGHEYTVKNLQFALTVEPD
Sbjct: 131 VFTGDTLFIAGCGKFFEGTAEQMYQSLCVTLGSLPKPTLVYCGHEYTVKNLQFALTVEPD 190

Query: 388 NAKIAQKLAWAEHQRRVGLPTIPSTIQEEFESNPFMRVDLPEVQDKVGCKSPVEALREIR 209
           NAKIAQKLAWAE QRRVGLPTIPSTIQEEFESNPFMRVDLPEVQ K G    ++   +  
Sbjct: 191 NAKIAQKLAWAERQRRVGLPTIPSTIQEEFESNPFMRVDLPEVQIKWGASLLLKPCVK-S 249

Query: 208 QRKDNWKG 185
             KDNWKG
Sbjct: 250 ATKDNWKG 257


>gb|EMJ19420.1| hypothetical protein PRUPE_ppa007897mg [Prunus persica]
          Length = 352

 Score =  441 bits (1133), Expect = e-121
 Identities = 201/248 (81%), Positives = 229/248 (92%)
 Frame = -3

Query: 928 DNYSYMIIDENTKEAAVVDPVEPHKVLRVAQENEVNLKFVLTTHHHWDHAGGNDKIKQLV 749
           DNY+Y+IIDE+TKEAA VDPVEP KVL+ AQE+ V++K VLTTHHHWDHAGGN+KIKQLV
Sbjct: 105 DNYAYLIIDESTKEAAAVDPVEPEKVLKAAQEHGVDIKLVLTTHHHWDHAGGNEKIKQLV 164

Query: 748 PGIKIYGGSVDNVRGCTDKVENGDKVSLGADISILSLHTPAHTKGHISYYITDKEGEDPA 569
           PGIKIYGGS+DNV+GCTDKV+NGDK+SLGAD+ ILSLHTP HTKGHISYY+T+KEG+DP 
Sbjct: 165 PGIKIYGGSIDNVKGCTDKVDNGDKISLGADVHILSLHTPCHTKGHISYYVTNKEGDDPT 224

Query: 568 VFTGDTLFIAGCGKFFEGTAEQMYQSLCVTLGSLPNPTRVFCGHEYTVKNLQFALTVEPD 389
           VFTGDTLFIAGCGKFFEGTAEQM+QSLCVTLGSLP PTRV+CGHEYTVKNLQFALTVEP 
Sbjct: 225 VFTGDTLFIAGCGKFFEGTAEQMHQSLCVTLGSLPKPTRVYCGHEYTVKNLQFALTVEPG 284

Query: 388 NAKIAQKLAWAEHQRRVGLPTIPSTIQEEFESNPFMRVDLPEVQDKVGCKSPVEALREIR 209
           N KI QKL+WA+ QR  GLPT+PSTI+EE E+NPFMR DLPE+Q++VGCK+ VEALREIR
Sbjct: 285 NEKIKQKLSWAQKQRETGLPTVPSTIEEEMETNPFMRADLPELQERVGCKTAVEALREIR 344

Query: 208 QRKDNWKG 185
           QRKD W+G
Sbjct: 345 QRKDTWRG 352


>gb|EXC21105.1| Hydroxyacylglutathione hydrolase cytoplasmic [Morus notabilis]
          Length = 258

 Score =  438 bits (1127), Expect = e-120
 Identities = 201/248 (81%), Positives = 229/248 (92%)
 Frame = -3

Query: 928 DNYSYMIIDENTKEAAVVDPVEPHKVLRVAQENEVNLKFVLTTHHHWDHAGGNDKIKQLV 749
           DNYSY+IIDE+TKEAAVVDPVEP KV+ VAQ++ V LK VLTTHHHWDHAGGN+K+KQLV
Sbjct: 11  DNYSYLIIDESTKEAAVVDPVEPEKVVGVAQQHGVLLKLVLTTHHHWDHAGGNEKLKQLV 70

Query: 748 PGIKIYGGSVDNVRGCTDKVENGDKVSLGADISILSLHTPAHTKGHISYYITDKEGEDPA 569
           PGIK+YGGS DNV+GCTD VENGDK+SLGAD++ILSLHTP HTKGH+SYY+T KEGEDPA
Sbjct: 71  PGIKVYGGSKDNVKGCTDSVENGDKISLGADVNILSLHTPCHTKGHVSYYVTSKEGEDPA 130

Query: 568 VFTGDTLFIAGCGKFFEGTAEQMYQSLCVTLGSLPNPTRVFCGHEYTVKNLQFALTVEPD 389
           VFTGDTLF+AGCGKFFEGTAEQM+QSLCVTL SLP PTRV+CGHEYTVKNLQFALTVEPD
Sbjct: 131 VFTGDTLFVAGCGKFFEGTAEQMHQSLCVTLASLPKPTRVYCGHEYTVKNLQFALTVEPD 190

Query: 388 NAKIAQKLAWAEHQRRVGLPTIPSTIQEEFESNPFMRVDLPEVQDKVGCKSPVEALREIR 209
           NA+I QKL+WA+ QR+ GLPTIPSTI+EE E+NPFMRVDLP++Q KVGC S VEA+REIR
Sbjct: 191 NARIQQKLSWAQQQRQAGLPTIPSTIEEELETNPFMRVDLPDIQKKVGCHSAVEAIREIR 250

Query: 208 QRKDNWKG 185
           + KDNW+G
Sbjct: 251 RLKDNWRG 258


>ref|XP_002329233.1| predicted protein [Populus trichocarpa]
           gi|566237931|ref|XP_006371323.1| Hydroxyacylglutathione
           hydrolase cytoplasmic family protein [Populus
           trichocarpa] gi|550317076|gb|ERP49120.1|
           Hydroxyacylglutathione hydrolase cytoplasmic family
           protein [Populus trichocarpa]
          Length = 258

 Score =  434 bits (1117), Expect = e-119
 Identities = 199/248 (80%), Positives = 229/248 (92%)
 Frame = -3

Query: 928 DNYSYMIIDENTKEAAVVDPVEPHKVLRVAQENEVNLKFVLTTHHHWDHAGGNDKIKQLV 749
           DNYSY+IIDE+TKEAAVVDPVEP K++  A E+ ++LK VLTTHHHWDHAGGN+KIK++V
Sbjct: 11  DNYSYLIIDESTKEAAVVDPVEPEKLVNAANEHGLHLKLVLTTHHHWDHAGGNEKIKEMV 70

Query: 748 PGIKIYGGSVDNVRGCTDKVENGDKVSLGADISILSLHTPAHTKGHISYYITDKEGEDPA 569
           PGIK+YGGS+DNV+GCT+KVENGDKVSLGAD++IL+LHTP HTKGHISYY+T KEGEDPA
Sbjct: 71  PGIKVYGGSLDNVKGCTNKVENGDKVSLGADVNILALHTPCHTKGHISYYVTGKEGEDPA 130

Query: 568 VFTGDTLFIAGCGKFFEGTAEQMYQSLCVTLGSLPNPTRVFCGHEYTVKNLQFALTVEPD 389
           VFTGDTLFIAGCGKFFEGTAEQMYQSLCVTLGSLP PT+V+CGHEYTVKNLQFALTVEPD
Sbjct: 131 VFTGDTLFIAGCGKFFEGTAEQMYQSLCVTLGSLPKPTQVYCGHEYTVKNLQFALTVEPD 190

Query: 388 NAKIAQKLAWAEHQRRVGLPTIPSTIQEEFESNPFMRVDLPEVQDKVGCKSPVEALREIR 209
           + KI +KL+WA+ QRR GLPTIPSTI+EE E+NPFMR +LP+VQ KVGC SPVEALR+IR
Sbjct: 191 SVKIQEKLSWAQKQRRAGLPTIPSTIEEEMETNPFMRAELPDVQAKVGCHSPVEALRKIR 250

Query: 208 QRKDNWKG 185
             KDNW+G
Sbjct: 251 LMKDNWRG 258


>ref|XP_004307750.1| PREDICTED: hydroxyacylglutathione hydrolase cytoplasmic-like
           isoform 2 [Fragaria vesca subsp. vesca]
          Length = 258

 Score =  432 bits (1111), Expect = e-119
 Identities = 196/248 (79%), Positives = 226/248 (91%)
 Frame = -3

Query: 928 DNYSYMIIDENTKEAAVVDPVEPHKVLRVAQENEVNLKFVLTTHHHWDHAGGNDKIKQLV 749
           DNY+Y+IIDE+TKEAAVVDPVEP KVL+ AQE+   +K VLTTHHHWDHAGGN+KIK+LV
Sbjct: 11  DNYAYLIIDESTKEAAVVDPVEPEKVLKAAQEHGAQVKIVLTTHHHWDHAGGNEKIKELV 70

Query: 748 PGIKIYGGSVDNVRGCTDKVENGDKVSLGADISILSLHTPAHTKGHISYYITDKEGEDPA 569
           PGIK+YGGSVDNV+GCTDKV+NGDK++LGAD  ILSLHTP HTKGHISYY+T KEGE+PA
Sbjct: 71  PGIKVYGGSVDNVKGCTDKVDNGDKITLGADTQILSLHTPCHTKGHISYYVTSKEGEEPA 130

Query: 568 VFTGDTLFIAGCGKFFEGTAEQMYQSLCVTLGSLPNPTRVFCGHEYTVKNLQFALTVEPD 389
           VFTGDTLF+AGCGKFFEGTAEQM+QSLCVTL SLP PTRV+CGHEYTVKNLQFA TVEP 
Sbjct: 131 VFTGDTLFVAGCGKFFEGTAEQMHQSLCVTLASLPKPTRVYCGHEYTVKNLQFAQTVEPH 190

Query: 388 NAKIAQKLAWAEHQRRVGLPTIPSTIQEEFESNPFMRVDLPEVQDKVGCKSPVEALREIR 209
           NAKI QKL+WA+ QR  GLPT+PSTI+ E E+NPF+R D+PE+Q+KVGC++ VEALREIR
Sbjct: 191 NAKIKQKLSWAQQQREAGLPTVPSTIESEMETNPFVRADMPELQEKVGCQNAVEALREIR 250

Query: 208 QRKDNWKG 185
           QRKDNW+G
Sbjct: 251 QRKDNWRG 258


>gb|EOY20210.1| Metallo-hydrolase/oxidoreductase superfamily protein [Theobroma
           cacao]
          Length = 258

 Score =  429 bits (1102), Expect = e-117
 Identities = 197/248 (79%), Positives = 223/248 (89%)
 Frame = -3

Query: 928 DNYSYMIIDENTKEAAVVDPVEPHKVLRVAQENEVNLKFVLTTHHHWDHAGGNDKIKQLV 749
           DNYSY+IIDE+TKEAA VDP EP KV+  A +  V LK VLTTHHHWDHAGGNDKIK+LV
Sbjct: 11  DNYSYLIIDESTKEAAAVDPAEPQKVVEAANQQGVVLKLVLTTHHHWDHAGGNDKIKELV 70

Query: 748 PGIKIYGGSVDNVRGCTDKVENGDKVSLGADISILSLHTPAHTKGHISYYITDKEGEDPA 569
           PGIK+YGGS+DNV+GCTD+++NGD + L + I++LSLHTP HTKGHISYY+T KEGEDPA
Sbjct: 71  PGIKVYGGSLDNVKGCTDQLQNGDNLQLSSHINVLSLHTPCHTKGHISYYVTGKEGEDPA 130

Query: 568 VFTGDTLFIAGCGKFFEGTAEQMYQSLCVTLGSLPNPTRVFCGHEYTVKNLQFALTVEPD 389
           VFTGDTLFIAGCGKFFEGTAEQMYQSLCVTLGSLP PTRVFCGHEYTVKNLQFALTVEP 
Sbjct: 131 VFTGDTLFIAGCGKFFEGTAEQMYQSLCVTLGSLPKPTRVFCGHEYTVKNLQFALTVEPK 190

Query: 388 NAKIAQKLAWAEHQRRVGLPTIPSTIQEEFESNPFMRVDLPEVQDKVGCKSPVEALREIR 209
           NA+I QKLAWA  QR+ GLPTIPSTI+EE E+NPFMRVDLPE+Q ++GC+SPVEALREIR
Sbjct: 191 NARIQQKLAWATSQRQAGLPTIPSTIEEELETNPFMRVDLPELQGRIGCQSPVEALREIR 250

Query: 208 QRKDNWKG 185
           + KDNW+G
Sbjct: 251 RMKDNWRG 258


>gb|AFK42856.1| unknown [Lotus japonicus]
          Length = 258

 Score =  424 bits (1091), Expect = e-116
 Identities = 197/248 (79%), Positives = 222/248 (89%)
 Frame = -3

Query: 928 DNYSYMIIDENTKEAAVVDPVEPHKVLRVAQENEVNLKFVLTTHHHWDHAGGNDKIKQLV 749
           DNYSY+I DE+TKEAAVVDPVEP KVL  A  + + LK VLTTHHHWDHAGGN+KIK+LV
Sbjct: 11  DNYSYLIEDESTKEAAVVDPVEPEKVLEAANLHGLTLKLVLTTHHHWDHAGGNEKIKELV 70

Query: 748 PGIKIYGGSVDNVRGCTDKVENGDKVSLGADISILSLHTPAHTKGHISYYITDKEGEDPA 569
           PGIK+YGGS+D V+GCTDKVENGDKVSLGADISIL+LHTP HTKGHISYY+T KE EDPA
Sbjct: 71  PGIKVYGGSIDKVKGCTDKVENGDKVSLGADISILALHTPCHTKGHISYYVTGKEDEDPA 130

Query: 568 VFTGDTLFIAGCGKFFEGTAEQMYQSLCVTLGSLPNPTRVFCGHEYTVKNLQFALTVEPD 389
           VFTGDTLFIA CGKFFEGTAEQMYQSLCVTLGSLP PTRV+CGHEY+VKNLQFALT+EP 
Sbjct: 131 VFTGDTLFIASCGKFFEGTAEQMYQSLCVTLGSLPKPTRVYCGHEYSVKNLQFALTIEPG 190

Query: 388 NAKIAQKLAWAEHQRRVGLPTIPSTIQEEFESNPFMRVDLPEVQDKVGCKSPVEALREIR 209
           N K  QKL WA++QR+ G PTIPSTI+EE E+NPFMR+DLPE+Q+KVG KSPVEAL E+R
Sbjct: 191 NLKTQQKLTWAQNQRQAGQPTIPSTIEEELETNPFMRIDLPEIQEKVGFKSPVEALGELR 250

Query: 208 QRKDNWKG 185
           + KDNW+G
Sbjct: 251 KLKDNWRG 258


>ref|XP_002267471.1| PREDICTED: hydroxyacylglutathione hydrolase cytoplasmic [Vitis
           vinifera] gi|297736644|emb|CBI25515.3| unnamed protein
           product [Vitis vinifera]
          Length = 258

 Score =  423 bits (1087), Expect = e-116
 Identities = 190/248 (76%), Positives = 228/248 (91%)
 Frame = -3

Query: 928 DNYSYMIIDENTKEAAVVDPVEPHKVLRVAQENEVNLKFVLTTHHHWDHAGGNDKIKQLV 749
           DNYSY+IIDE++KEAAVVDPVEP KVL+ A E  V+LK VLTTHHHWDHAGGN+KIKQLV
Sbjct: 11  DNYSYLIIDESSKEAAVVDPVEPQKVLQAAYEYGVHLKLVLTTHHHWDHAGGNEKIKQLV 70

Query: 748 PGIKIYGGSVDNVRGCTDKVENGDKVSLGADISILSLHTPAHTKGHISYYITDKEGEDPA 569
           PGI++YGGSVDNV+GCT  ++NGDK+SLG+D+++L+LHTP HT+GHISYY+T KE + PA
Sbjct: 71  PGIEVYGGSVDNVKGCTHPLQNGDKLSLGSDLAVLALHTPCHTRGHISYYVTGKEEDVPA 130

Query: 568 VFTGDTLFIAGCGKFFEGTAEQMYQSLCVTLGSLPNPTRVFCGHEYTVKNLQFALTVEPD 389
           VFTGDTLF+AGCGKFFEGTAEQMYQSLCVTL SLP PTRV+CGHEYTVKNLQFALTVEPD
Sbjct: 131 VFTGDTLFVAGCGKFFEGTAEQMYQSLCVTLASLPKPTRVYCGHEYTVKNLQFALTVEPD 190

Query: 388 NAKIAQKLAWAEHQRRVGLPTIPSTIQEEFESNPFMRVDLPEVQDKVGCKSPVEALREIR 209
           N ++ QKL+WA+HQR+ GLPTIPSTI EE E+NPFMRVDLPE+Q++VGC+S ++AL+EIR
Sbjct: 191 NVRVGQKLSWAQHQRQAGLPTIPSTIDEEMETNPFMRVDLPELQERVGCQSAIDALQEIR 250

Query: 208 QRKDNWKG 185
           ++KDNW+G
Sbjct: 251 RQKDNWRG 258


>ref|XP_004146429.1| PREDICTED: hydroxyacylglutathione hydrolase cytoplasmic-like
           [Cucumis sativus]
          Length = 258

 Score =  421 bits (1082), Expect = e-115
 Identities = 193/248 (77%), Positives = 222/248 (89%)
 Frame = -3

Query: 928 DNYSYMIIDENTKEAAVVDPVEPHKVLRVAQENEVNLKFVLTTHHHWDHAGGNDKIKQLV 749
           DNYSY+IIDE+TKEAAVVDPVEP K++ +A E+ V+LK VLTTHHHWDH+GGN+KIKQLV
Sbjct: 11  DNYSYLIIDESTKEAAVVDPVEPEKIVNIANEHGVHLKLVLTTHHHWDHSGGNEKIKQLV 70

Query: 748 PGIKIYGGSVDNVRGCTDKVENGDKVSLGADISILSLHTPAHTKGHISYYITDKEGEDPA 569
           PGIK+YGGS+D V+GCTD VENGDK+ LGAD+ ILSLHTP HTKGHISYY++ KEGEDPA
Sbjct: 71  PGIKVYGGSIDKVKGCTDAVENGDKIPLGADVVILSLHTPCHTKGHISYYVSGKEGEDPA 130

Query: 568 VFTGDTLFIAGCGKFFEGTAEQMYQSLCVTLGSLPNPTRVFCGHEYTVKNLQFALTVEPD 389
           VFTGDTLF+AGCGKFFEGTAEQMYQSLC+ LGSLP  TRV+CGHEYTVKNLQFALTVEPD
Sbjct: 131 VFTGDTLFVAGCGKFFEGTAEQMYQSLCIKLGSLPKQTRVYCGHEYTVKNLQFALTVEPD 190

Query: 388 NAKIAQKLAWAEHQRRVGLPTIPSTIQEEFESNPFMRVDLPEVQDKVGCKSPVEALREIR 209
           N KI  KL WA+ QR+ G  T+PSTI+EE E+NPFMRVDLP++Q+KVG  SPVEA+REIR
Sbjct: 191 NEKIKGKLLWAQGQRQAGQATVPSTIEEEMETNPFMRVDLPQLQEKVGNHSPVEAIREIR 250

Query: 208 QRKDNWKG 185
           + KDNWKG
Sbjct: 251 RLKDNWKG 258


>ref|XP_006486126.1| PREDICTED: hydroxyacylglutathione hydrolase cytoplasmic-like
           [Citrus sinensis]
          Length = 258

 Score =  420 bits (1080), Expect = e-115
 Identities = 188/248 (75%), Positives = 225/248 (90%)
 Frame = -3

Query: 928 DNYSYMIIDENTKEAAVVDPVEPHKVLRVAQENEVNLKFVLTTHHHWDHAGGNDKIKQLV 749
           DNY+Y++IDE TKEAAVVDPVEP K++  A+++ VNL  VLTTHHHWDHAGGN+K+K++V
Sbjct: 11  DNYAYLVIDETTKEAAVVDPVEPEKIIEAAKQHGVNLTTVLTTHHHWDHAGGNEKMKEMV 70

Query: 748 PGIKIYGGSVDNVRGCTDKVENGDKVSLGADISILSLHTPAHTKGHISYYITDKEGEDPA 569
           PGIK+YGGS+DNV+GCT +VENGDK S+GA +++LSLHTP HTKGHISYY+T KEGEDPA
Sbjct: 71  PGIKVYGGSLDNVKGCTHQVENGDKFSIGAHVNVLSLHTPCHTKGHISYYVTGKEGEDPA 130

Query: 568 VFTGDTLFIAGCGKFFEGTAEQMYQSLCVTLGSLPNPTRVFCGHEYTVKNLQFALTVEPD 389
           VFTGDTLF+AGCGKFFEG+AEQMYQSLCVTLGSLP PT+V+CGHEYTVKNL FALTVEP 
Sbjct: 131 VFTGDTLFVAGCGKFFEGSAEQMYQSLCVTLGSLPQPTQVYCGHEYTVKNLLFALTVEPS 190

Query: 388 NAKIAQKLAWAEHQRRVGLPTIPSTIQEEFESNPFMRVDLPEVQDKVGCKSPVEALREIR 209
           N K+ QKLAWA++Q++ GLPTIPSTI+EE E+NPFMRVDLPE+Q+ VG   P+EALREIR
Sbjct: 191 NVKLQQKLAWAQNQQQAGLPTIPSTIEEELETNPFMRVDLPELQELVGFNDPIEALREIR 250

Query: 208 QRKDNWKG 185
           +RKDNW+G
Sbjct: 251 KRKDNWRG 258


>ref|XP_006435967.1| hypothetical protein CICLE_v10032268mg [Citrus clementina]
           gi|557538163|gb|ESR49207.1| hypothetical protein
           CICLE_v10032268mg [Citrus clementina]
          Length = 295

 Score =  420 bits (1080), Expect = e-115
 Identities = 188/248 (75%), Positives = 225/248 (90%)
 Frame = -3

Query: 928 DNYSYMIIDENTKEAAVVDPVEPHKVLRVAQENEVNLKFVLTTHHHWDHAGGNDKIKQLV 749
           DNY+Y++IDE TKEAAVVDPVEP K++  A+++ VNL  VLTTHHHWDHAGGN+K+K++V
Sbjct: 48  DNYAYLVIDETTKEAAVVDPVEPEKIIEAAKQHGVNLTTVLTTHHHWDHAGGNEKMKEMV 107

Query: 748 PGIKIYGGSVDNVRGCTDKVENGDKVSLGADISILSLHTPAHTKGHISYYITDKEGEDPA 569
           PGIK+YGGS+DNV+GCT +VENGDK S+GA +++LSLHTP HTKGHISYY+T KEGEDPA
Sbjct: 108 PGIKVYGGSLDNVKGCTHQVENGDKFSIGAHVNVLSLHTPCHTKGHISYYVTGKEGEDPA 167

Query: 568 VFTGDTLFIAGCGKFFEGTAEQMYQSLCVTLGSLPNPTRVFCGHEYTVKNLQFALTVEPD 389
           VFTGDTLF+AGCGKFFEG+AEQMYQSLCVTLGSLP PT+V+CGHEYTVKNL FALTVEP 
Sbjct: 168 VFTGDTLFVAGCGKFFEGSAEQMYQSLCVTLGSLPQPTQVYCGHEYTVKNLLFALTVEPS 227

Query: 388 NAKIAQKLAWAEHQRRVGLPTIPSTIQEEFESNPFMRVDLPEVQDKVGCKSPVEALREIR 209
           N K+ QKLAWA++Q++ GLPTIPSTI+EE E+NPFMRVDLPE+Q+ VG   P+EALREIR
Sbjct: 228 NVKLQQKLAWAQNQQQAGLPTIPSTIEEELETNPFMRVDLPELQELVGFNDPIEALREIR 287

Query: 208 QRKDNWKG 185
           +RKDNW+G
Sbjct: 288 KRKDNWRG 295


>ref|XP_004490514.1| PREDICTED: hydroxyacylglutathione hydrolase cytoplasmic-like [Cicer
           arietinum]
          Length = 258

 Score =  419 bits (1076), Expect = e-114
 Identities = 194/248 (78%), Positives = 221/248 (89%)
 Frame = -3

Query: 928 DNYSYMIIDENTKEAAVVDPVEPHKVLRVAQENEVNLKFVLTTHHHWDHAGGNDKIKQLV 749
           DNYSY+I++E+TKEAA VDPVEP KVL  +    + LKFVLTTHHHWDHAGGNDKIK+LV
Sbjct: 11  DNYSYLIVNESTKEAAAVDPVEPEKVLEASNSYGLTLKFVLTTHHHWDHAGGNDKIKELV 70

Query: 748 PGIKIYGGSVDNVRGCTDKVENGDKVSLGADISILSLHTPAHTKGHISYYITDKEGEDPA 569
           PGIK+YGGS+DNV+GCTD VENGDKV LGADI++L+LHTP HTKGHISYY+T KE EDPA
Sbjct: 71  PGIKVYGGSLDNVKGCTDAVENGDKVHLGADITVLALHTPCHTKGHISYYVTGKEDEDPA 130

Query: 568 VFTGDTLFIAGCGKFFEGTAEQMYQSLCVTLGSLPNPTRVFCGHEYTVKNLQFALTVEPD 389
           VFTGDTLFIAGCGKFFEGTAEQMYQSL VTLGSLP PTRV+CGHEYT KNLQFALTVEPD
Sbjct: 131 VFTGDTLFIAGCGKFFEGTAEQMYQSLNVTLGSLPKPTRVYCGHEYTAKNLQFALTVEPD 190

Query: 388 NAKIAQKLAWAEHQRRVGLPTIPSTIQEEFESNPFMRVDLPEVQDKVGCKSPVEALREIR 209
           N KI QKL WA++QR+VG PTIPSTI++E E+NPFMRVDL  +Q+K+G KSPV+AL E+R
Sbjct: 191 NLKILQKLTWAQNQRQVGQPTIPSTIEDELETNPFMRVDLHAIQEKMGSKSPVQALGELR 250

Query: 208 QRKDNWKG 185
           + KDNWKG
Sbjct: 251 KLKDNWKG 258


>ref|XP_006595529.1| PREDICTED: uncharacterized protein LOC100527131 isoform X1 [Glycine
           max]
          Length = 258

 Score =  418 bits (1075), Expect = e-114
 Identities = 193/248 (77%), Positives = 222/248 (89%)
 Frame = -3

Query: 928 DNYSYMIIDENTKEAAVVDPVEPHKVLRVAQENEVNLKFVLTTHHHWDHAGGNDKIKQLV 749
           DNYSY+I+DE+TKEAA VDPVEP KVL VA  + + LKFVLTTHHHWDHAGGNDKIKQLV
Sbjct: 11  DNYSYLIVDESTKEAAAVDPVEPEKVLEVASSHGLTLKFVLTTHHHWDHAGGNDKIKQLV 70

Query: 748 PGIKIYGGSVDNVRGCTDKVENGDKVSLGADISILSLHTPAHTKGHISYYITDKEGEDPA 569
           PGIK+YGGS++NV+GCTDKVENGDKVSLGA+I+IL+LHTP HT+GHISYY+T KE E PA
Sbjct: 71  PGIKVYGGSIENVKGCTDKVENGDKVSLGAEITILALHTPCHTQGHISYYVTGKEDEQPA 130

Query: 568 VFTGDTLFIAGCGKFFEGTAEQMYQSLCVTLGSLPNPTRVFCGHEYTVKNLQFALTVEPD 389
           VFTGDTLFIA CGKFFEGTAEQMYQSL VTL SLP  TRV+CGHEY+V NLQFALT+EPD
Sbjct: 131 VFTGDTLFIASCGKFFEGTAEQMYQSLNVTLASLPKSTRVYCGHEYSVNNLQFALTLEPD 190

Query: 388 NAKIAQKLAWAEHQRRVGLPTIPSTIQEEFESNPFMRVDLPEVQDKVGCKSPVEALREIR 209
           N +I QKL WA +QR+ G  TIPSTI++E E+NPFMRVDLPE+Q++VGCKSPVEAL EIR
Sbjct: 191 NLRIQQKLTWARNQRQAGQATIPSTIEDELETNPFMRVDLPEIQERVGCKSPVEALGEIR 250

Query: 208 QRKDNWKG 185
           ++KDNW+G
Sbjct: 251 KQKDNWRG 258


>gb|AGV54469.1| hydroxyacylglutathione hydrolase [Phaseolus vulgaris]
           gi|561014468|gb|ESW13329.1| hypothetical protein
           PHAVU_008G187100g [Phaseolus vulgaris]
          Length = 258

 Score =  417 bits (1073), Expect = e-114
 Identities = 190/248 (76%), Positives = 222/248 (89%)
 Frame = -3

Query: 928 DNYSYMIIDENTKEAAVVDPVEPHKVLRVAQENEVNLKFVLTTHHHWDHAGGNDKIKQLV 749
           DNYSY+I+DE+TKEAA VDPVEP K+L  A  + + LK VLTTHHHWDHAGGN+KIKQLV
Sbjct: 11  DNYSYLIVDESTKEAAAVDPVEPEKILEAANSHGLTLKLVLTTHHHWDHAGGNEKIKQLV 70

Query: 748 PGIKIYGGSVDNVRGCTDKVENGDKVSLGADISILSLHTPAHTKGHISYYITDKEGEDPA 569
           PGIK+YGGS+DNV+GCTDKVENGDKVSLGAD++IL+LHTP HT+GHISYY+T KE E PA
Sbjct: 71  PGIKVYGGSIDNVKGCTDKVENGDKVSLGADVTILALHTPCHTQGHISYYVTGKEDEHPA 130

Query: 568 VFTGDTLFIAGCGKFFEGTAEQMYQSLCVTLGSLPNPTRVFCGHEYTVKNLQFALTVEPD 389
           VFTGDTLFIA CGKFFEGTAEQMYQSL VTL SLP  TRV+CGHEY+VKNLQFALT+EPD
Sbjct: 131 VFTGDTLFIASCGKFFEGTAEQMYQSLNVTLASLPKTTRVYCGHEYSVKNLQFALTLEPD 190

Query: 388 NAKIAQKLAWAEHQRRVGLPTIPSTIQEEFESNPFMRVDLPEVQDKVGCKSPVEALREIR 209
           N++  QKL WA++QR+ G  T+PSTI++E E+NPFMRVDLPE+Q+KVGCKSPVEAL EIR
Sbjct: 191 NSRTQQKLTWAQNQRQAGQATVPSTIEDELETNPFMRVDLPEIQEKVGCKSPVEALGEIR 250

Query: 208 QRKDNWKG 185
           ++KDNW+G
Sbjct: 251 KKKDNWRG 258


>ref|XP_003615463.1| Hydroxyacylglutathione hydrolase [Medicago truncatula]
           gi|355516798|gb|AES98421.1| Hydroxyacylglutathione
           hydrolase [Medicago truncatula]
          Length = 258

 Score =  413 bits (1062), Expect = e-113
 Identities = 190/248 (76%), Positives = 220/248 (88%)
 Frame = -3

Query: 928 DNYSYMIIDENTKEAAVVDPVEPHKVLRVAQENEVNLKFVLTTHHHWDHAGGNDKIKQLV 749
           DNYSY+I+DE+TKEAA VDPVEP KVL  +    + +KFVLTTHHHWDHAGGN+KIK+LV
Sbjct: 11  DNYSYLIVDESTKEAAAVDPVEPEKVLEASNSLGLTIKFVLTTHHHWDHAGGNEKIKELV 70

Query: 748 PGIKIYGGSVDNVRGCTDKVENGDKVSLGADISILSLHTPAHTKGHISYYITDKEGEDPA 569
           PGIK+YGGS+DNV+GCT+ +ENGDKV LGADI+IL+LHTP HTKGHISYY+T KE EDPA
Sbjct: 71  PGIKVYGGSIDNVKGCTNALENGDKVHLGADINILALHTPCHTKGHISYYVTGKEDEDPA 130

Query: 568 VFTGDTLFIAGCGKFFEGTAEQMYQSLCVTLGSLPNPTRVFCGHEYTVKNLQFALTVEPD 389
           VFTGDTLFIAGCGKFFEGTAEQMYQSL VTLGSLP PTRV+CGHEY+VKNLQFALTVEPD
Sbjct: 131 VFTGDTLFIAGCGKFFEGTAEQMYQSLSVTLGSLPKPTRVYCGHEYSVKNLQFALTVEPD 190

Query: 388 NAKIAQKLAWAEHQRRVGLPTIPSTIQEEFESNPFMRVDLPEVQDKVGCKSPVEALREIR 209
           N +I +KL WA++QR+ G PTIPSTI +E ESNPFMRVDLP +Q+K+G  SPVEAL E+R
Sbjct: 191 NLRILEKLTWAQNQRQTGQPTIPSTIGDELESNPFMRVDLPAIQEKMGFNSPVEALGELR 250

Query: 208 QRKDNWKG 185
           + KDNW+G
Sbjct: 251 KVKDNWRG 258


>ref|NP_187696.1| hydroxyacylglutathione hydrolase [Arabidopsis thaliana]
           gi|3913733|sp|O24496.2|GLO2C_ARATH RecName:
           Full=Hydroxyacylglutathione hydrolase cytoplasmic;
           AltName: Full=Glyoxalase II; Short=Glx II
           gi|6630545|gb|AAF19564.1|AC011708_7
           hydroxyacylglutathione hydrolase cytoplasmic (glyoxalase
           II) (GLX II) [Arabidopsis thaliana]
           gi|1924921|emb|CAA69644.1| hydroxyacylglutathione
           hydrolase [Arabidopsis thaliana]
           gi|15450457|gb|AAK96522.1| AT3g10850/T7M13_7
           [Arabidopsis thaliana] gi|23507765|gb|AAN38686.1|
           At3g10850/T7M13_7 [Arabidopsis thaliana]
           gi|110737017|dbj|BAF00463.1| hydroxyacylglutathione
           hydrolase cytoplasmic [Arabidopsis thaliana]
           gi|332641442|gb|AEE74963.1| hydroxyacylglutathione
           hydrolase [Arabidopsis thaliana]
          Length = 258

 Score =  412 bits (1059), Expect = e-113
 Identities = 182/248 (73%), Positives = 222/248 (89%)
 Frame = -3

Query: 928 DNYSYMIIDENTKEAAVVDPVEPHKVLRVAQENEVNLKFVLTTHHHWDHAGGNDKIKQLV 749
           DNYSY+IIDE+T +AAVVDPV+P KV+  A++++  +KFVLTTHHHWDHAGGN+KIKQLV
Sbjct: 11  DNYSYLIIDESTGDAAVVDPVDPEKVIASAEKHQAKIKFVLTTHHHWDHAGGNEKIKQLV 70

Query: 748 PGIKIYGGSVDNVRGCTDKVENGDKVSLGADISILSLHTPAHTKGHISYYITDKEGEDPA 569
           P IK+YGGS+D V+GCTD V+NGDK++LG DI+IL+LHTP HTKGHISYY+  KEGE+PA
Sbjct: 71  PDIKVYGGSLDKVKGCTDAVDNGDKLTLGQDINILALHTPCHTKGHISYYVNGKEGENPA 130

Query: 568 VFTGDTLFIAGCGKFFEGTAEQMYQSLCVTLGSLPNPTRVFCGHEYTVKNLQFALTVEPD 389
           VFTGDTLF+AGCGKFFEGTAEQMYQSLCVTL +LP PT+V+CGHEYTVKNL+FALTVEP+
Sbjct: 131 VFTGDTLFVAGCGKFFEGTAEQMYQSLCVTLAALPKPTQVYCGHEYTVKNLEFALTVEPN 190

Query: 388 NAKIAQKLAWAEHQRRVGLPTIPSTIQEEFESNPFMRVDLPEVQDKVGCKSPVEALREIR 209
           N KI QKLAWA  QR+  LPTIPST++EE E+NPFMRVD PE+Q+K+GCKSP++ +RE+R
Sbjct: 191 NGKIQQKLAWARQQRQADLPTIPSTLEEELETNPFMRVDKPEIQEKLGCKSPIDTMREVR 250

Query: 208 QRKDNWKG 185
            +KD W+G
Sbjct: 251 NKKDQWRG 258


>ref|XP_002884816.1| glyoxalase 2-2 [Arabidopsis lyrata subsp. lyrata]
           gi|297330656|gb|EFH61075.1| glyoxalase 2-2 [Arabidopsis
           lyrata subsp. lyrata]
          Length = 258

 Score =  410 bits (1055), Expect = e-112
 Identities = 182/248 (73%), Positives = 222/248 (89%)
 Frame = -3

Query: 928 DNYSYMIIDENTKEAAVVDPVEPHKVLRVAQENEVNLKFVLTTHHHWDHAGGNDKIKQLV 749
           DNYSY+IIDE+T +AAVVDPV+P KV+  A++++  +KFVLTTHHHWDHAGGN+KIKQLV
Sbjct: 11  DNYSYLIIDESTGDAAVVDPVDPEKVIASAEQHQAKIKFVLTTHHHWDHAGGNEKIKQLV 70

Query: 748 PGIKIYGGSVDNVRGCTDKVENGDKVSLGADISILSLHTPAHTKGHISYYITDKEGEDPA 569
           P IK+YGGS+D V+GCTD V+NGDK++LG D++IL+LHTP HTKGHISYY+T KEGE+PA
Sbjct: 71  PEIKVYGGSLDKVKGCTDAVDNGDKLTLGQDVNILALHTPCHTKGHISYYVTGKEGENPA 130

Query: 568 VFTGDTLFIAGCGKFFEGTAEQMYQSLCVTLGSLPNPTRVFCGHEYTVKNLQFALTVEPD 389
           VFTGDTLF+AGCGKFFEGTAEQMY+SLCVTL SLP PT+V+CGHEYTVKNL+FALTVEP+
Sbjct: 131 VFTGDTLFVAGCGKFFEGTAEQMYKSLCVTLASLPKPTQVYCGHEYTVKNLEFALTVEPN 190

Query: 388 NAKIAQKLAWAEHQRRVGLPTIPSTIQEEFESNPFMRVDLPEVQDKVGCKSPVEALREIR 209
           N KI QKLAWA  QR+  L TIPST++EE E+NPFMRVD PE+Q+K+GCKSP++ LRE+R
Sbjct: 191 NEKIQQKLAWARQQRQADLQTIPSTLEEELETNPFMRVDKPEIQEKLGCKSPIDTLREVR 250

Query: 208 QRKDNWKG 185
            +KD W+G
Sbjct: 251 NKKDQWRG 258