BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes22_contig00033883
         (534 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcocla...   149   3e-34
sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-...   149   3e-34
sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-...   148   6e-34
gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [P...   147   1e-33
ref|XP_002276320.2| PREDICTED: probable (S)-N-methylcoclaurine 3...   147   1e-33

>sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydroxylase
           isozyme 2; AltName: Full=Cytochrome P450 80B2
           gi|9971208|dbj|BAB12433.1|
           (S)-N-methylcoclaurine-3'-hydroxylase [Coptis japonica]
          Length = 488

 Score =  149 bits (376), Expect = 3e-34
 Identities = 71/149 (47%), Positives = 106/149 (71%)
 Frame = -1

Query: 450 VFLWFLIKQAKCFSLNNIKNLPPGPPKLPVIGNLHQVGEKPHVDTADFAKEYGPLISLRL 271
           +FL+F+++ ++       KNLPPGP   P++GNL Q+G+KPH + A  A++YG L SL+L
Sbjct: 15  LFLFFILRDSRP------KNLPPGPRPSPIVGNLLQLGDKPHAEFAKLAQKYGELFSLKL 68

Query: 270 GTQVLVVASSPETAMGILKTQDRLLCSRMVPTAFQHKSLLPHSLLWSDCNETWKNLRTLC 91
           G+Q +VVASSP  A  ILKT D++L  R V  +F+ K  + +S++WS+CN+ WK LR +C
Sbjct: 69  GSQTVVVASSPAAAAEILKTHDKILSGRYVFQSFRVKEHVENSIVWSECNDNWKLLRKVC 128

Query: 90  RTEMFSSKALEAQSRIREEKVDQLLDFLR 4
           RTE+F+ K +E+QS IRE K  +++ FLR
Sbjct: 129 RTELFTPKMIESQSEIREAKAREMVKFLR 157


>sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2;
           AltName: Full=Cytochrome P450 80B2
           gi|3127029|gb|AAC39453.1| (S)-N-methylcoclaurine
           3'-hydroxylase [Eschscholzia californica]
          Length = 488

 Score =  149 bits (375), Expect = 3e-34
 Identities = 65/138 (47%), Positives = 101/138 (73%)
 Frame = -1

Query: 414 FSLNNIKNLPPGPPKLPVIGNLHQVGEKPHVDTADFAKEYGPLISLRLGTQVLVVASSPE 235
           F  +  KNLPPGP   P++GNL Q+GEKPH   A+ A+ YG + +L++GT+ +VVAS+  
Sbjct: 21  FGSSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIFTLKMGTETVVVASTSS 80

Query: 234 TAMGILKTQDRLLCSRMVPTAFQHKSLLPHSLLWSDCNETWKNLRTLCRTEMFSSKALEA 55
            A  ILKT DR+L +R V  +F+ K  + +S++WSDC ETWKNLR +CRTE+F+ K +E+
Sbjct: 81  AASEILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTETWKNLRKVCRTELFTQKMIES 140

Query: 54  QSRIREEKVDQLLDFLRR 1
           Q+ +RE+K ++++++L +
Sbjct: 141 QAHVREKKCEEMVEYLMK 158


>sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
           AltName: Full=Cytochrome P450 80B1
           gi|3127027|gb|AAC39452.1| (S)-N-methylcoclaurine
           3'-hydroxylase [Eschscholzia californica]
          Length = 487

 Score =  148 bits (373), Expect = 6e-34
 Identities = 64/132 (48%), Positives = 99/132 (75%)
 Frame = -1

Query: 396 KNLPPGPPKLPVIGNLHQVGEKPHVDTADFAKEYGPLISLRLGTQVLVVASSPETAMGIL 217
           KNLPPGP   P++GNL Q+GEKPH   A+ A+ YG + +L++GT+ +VVAS+   A  IL
Sbjct: 26  KNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIFTLKMGTETVVVASTSSAASEIL 85

Query: 216 KTQDRLLCSRMVPTAFQHKSLLPHSLLWSDCNETWKNLRTLCRTEMFSSKALEAQSRIRE 37
           KT DR+L +R V  +F+ K  + +S++WSDC ETWKNLR +CRTE+F+ K +E+Q+ +RE
Sbjct: 86  KTHDRILSARYVFQSFRVKGHVENSIVWSDCTETWKNLRKVCRTELFTQKMIESQAHVRE 145

Query: 36  EKVDQLLDFLRR 1
           +K ++++++L +
Sbjct: 146 KKCEEMVEYLMK 157


>gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [Papaver bracteatum]
          Length = 486

 Score =  147 bits (371), Expect = 1e-33
 Identities = 72/160 (45%), Positives = 110/160 (68%)
 Frame = -1

Query: 480 VSPLCILLTPVFLWFLIKQAKCFSLNNIKNLPPGPPKLPVIGNLHQVGEKPHVDTADFAK 301
           VS + +++T  FL+ + + +      + K LPPGP   P++GNL Q+GEKPH   A+ AK
Sbjct: 5   VSLVAVVIT-TFLYLIFRDS------SPKGLPPGPKPWPIVGNLLQLGEKPHSQFAELAK 57

Query: 300 EYGPLISLRLGTQVLVVASSPETAMGILKTQDRLLCSRMVPTAFQHKSLLPHSLLWSDCN 121
            YG L +L+LG++ +VVAS+P  A  ILKT DR+L  R V  +F+ K  + +S++WS+CN
Sbjct: 58  TYGDLFTLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECN 117

Query: 120 ETWKNLRTLCRTEMFSSKALEAQSRIREEKVDQLLDFLRR 1
           ETWK LR +CR E+F+ K +E+Q+ IRE K  ++++FL+R
Sbjct: 118 ETWKKLRKVCRAELFTQKMIESQAEIRESKAMEMVEFLKR 157


>ref|XP_002276320.2| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
           2-like [Vitis vinifera]
          Length = 571

 Score =  147 bits (370), Expect = 1e-33
 Identities = 79/176 (44%), Positives = 117/176 (66%), Gaps = 3/176 (1%)
 Frame = -1

Query: 525 IIIMDITAFLRDNNVVSPLCILLTPVFLWFLIKQAKCFSLNNIKNLPPGPPKLPVIGNLH 346
           I +MD  A     N+  PL +LL P+ ++ ++K  K  S  +   LPPGP   P+IGN+H
Sbjct: 65  IKLMDEAALTEGTNLF-PLILLLLPL-IFLILKHLKSKSPIS---LPPGPYPWPIIGNVH 119

Query: 345 QVGEKPHVDTADFAKEYGPLISLRLGTQVLVVASSPETAMGILKTQDRLLCSRMVPTAFQ 166
           Q+G++ H+  ADFA+ YGPL SLRLGTQ L+V SS   A  IL + DR+ C+R VP    
Sbjct: 120 QIGKQRHIAMADFARSYGPLFSLRLGTQTLIVGSSAAAAKEILSSYDRIFCARYVPGVMP 179

Query: 165 HKS--LLPHSLLWS-DCNETWKNLRTLCRTEMFSSKALEAQSRIREEKVDQLLDFL 7
            KS     +S++WS +C++ WK LRT+CRT++FS KA+E+Q+ +RE+K+ +++ FL
Sbjct: 180 EKSSEFYNNSIVWSLECDDRWKYLRTMCRTQLFSGKAIESQACLREKKLMEVVGFL 235