BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes22_contig00033330
         (391 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002513348.1| (S)-N-methylcoclaurine 3'-hydroxylase isozym...   119   2e-25
ref|XP_002510830.1| (S)-N-methylcoclaurine 3'-hydroxylase isozym...   118   6e-25
ref|XP_002318833.1| cytochrome P450 [Populus trichocarpa] gi|222...   115   4e-24
emb|CBI31830.3| unnamed protein product [Vitis vinifera]              112   2e-23
ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   112   2e-23

>ref|XP_002513348.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative [Ricinus
           communis] gi|223547256|gb|EEF48751.1|
           (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative
           [Ricinus communis]
          Length = 426

 Score =  119 bits (299), Expect = 2e-25
 Identities = 60/128 (46%), Positives = 86/128 (67%)
 Frame = -3

Query: 386 AGGLREKLFKTLENAVAPNISDFFPMLQRLDLQGLRKETLKHMKEIFSSWEGIINERRLV 207
           AGGL+  + K ++ A APNI+DF+P+L RLD Q +R++     +++ ++W+  I ERR  
Sbjct: 137 AGGLKTLITKLMQLATAPNIADFYPVLARLDPQNMRRKMKIGFEKVLNAWQIYIKERREN 196

Query: 206 AVAKLEEQCFLDRLLENGFSNDQINTLALELFIAGTETTTLTIVWAMAELIKNKKVMVKV 27
            V    E  FLD  L  GF +DQIN L LELF AG +TTT T+ WAMAEL+K++  +VKV
Sbjct: 197 HVNDAPETDFLDVFLSTGFDDDQINWLVLELFAAGVDTTTTTVEWAMAELLKSRATLVKV 256

Query: 26  QEELRNKI 3
           Q+EL  ++
Sbjct: 257 QQELDREV 264


>ref|XP_002510830.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative [Ricinus
           communis] gi|223549945|gb|EEF51432.1|
           (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative
           [Ricinus communis]
          Length = 496

 Score =  118 bits (295), Expect = 6e-25
 Identities = 63/132 (47%), Positives = 88/132 (66%), Gaps = 5/132 (3%)
 Frame = -3

Query: 383 GGLREKLFKTLENAVAPNISDFFPMLQRLDLQGLRKETLKHMKEIFSSWEGIINERRLV- 207
           GG++  L+  +E A +PNI++F+P+L  LD QGL+++  K +KE+F  WE  I ERR   
Sbjct: 205 GGVKSLLWSMMEMATSPNIAEFYPILAPLDPQGLKRKMTKCLKEMFGVWEIYIKERRRTH 264

Query: 206 ----AVAKLEEQCFLDRLLENGFSNDQINTLALELFIAGTETTTLTIVWAMAELIKNKKV 39
               A  K +   FLD  L NGF +DQIN L +EL  AGT+TT+ ++ WAMAEL+KNK+ 
Sbjct: 265 DHDHAAPKTD---FLDIFLSNGFDDDQINWLVMELLSAGTDTTSTSVEWAMAELLKNKEA 321

Query: 38  MVKVQEELRNKI 3
           M KV+EEL  +I
Sbjct: 322 MKKVREELDREI 333


>ref|XP_002318833.1| cytochrome P450 [Populus trichocarpa] gi|222859506|gb|EEE97053.1|
           cytochrome P450 [Populus trichocarpa]
          Length = 503

 Score =  115 bits (288), Expect = 4e-24
 Identities = 62/128 (48%), Positives = 83/128 (64%)
 Frame = -3

Query: 386 AGGLREKLFKTLENAVAPNISDFFPMLQRLDLQGLRKETLKHMKEIFSSWEGIINERRLV 207
           + GL+  + K +     PNI+DF+P+   LD QGLR++  K ++E F+ W   I ERR  
Sbjct: 214 SSGLKSHVRKLMLLVATPNIADFYPIFAGLDPQGLRRKLSKLVEETFAIWAINIKERRNS 273

Query: 206 AVAKLEEQCFLDRLLENGFSNDQINTLALELFIAGTETTTLTIVWAMAELIKNKKVMVKV 27
            V    ++ FLD  L NGF +DQIN LA ELF AGT+TT  TI WA+AE++KNK+VM KV
Sbjct: 274 YVHDSPKRDFLDVFLANGFDDDQINWLAAELFSAGTDTTATTIEWAVAEILKNKEVMKKV 333

Query: 26  QEELRNKI 3
            EEL  +I
Sbjct: 334 DEELEREI 341


>emb|CBI31830.3| unnamed protein product [Vitis vinifera]
          Length = 1538

 Score =  112 bits (281), Expect = 2e-23
 Identities = 55/124 (44%), Positives = 83/124 (66%)
 Frame = -3

Query: 383  GGLREKLFKTLENAVAPNISDFFPMLQRLDLQGLRKETLKHMKEIFSSWEGIINERRLVA 204
            G L++ + K  E    PN+SD++P+L  LD+QG+ ++  +  + I ++WE I+ ERR   
Sbjct: 750  GALKDLIHKNAEFGATPNLSDYYPILGGLDIQGINRKAKEMFERIPTTWEDILKERRTQR 809

Query: 203  VAKLEEQCFLDRLLENGFSNDQINTLALELFIAGTETTTLTIVWAMAELIKNKKVMVKVQ 24
              +   + FL+ LLE GF +DQIN + LELF AG ET++LT+ WAMAELI+N+  M K++
Sbjct: 810  SNRSSHRDFLEALLEIGFEDDQINQVILELFSAGAETSSLTVEWAMAELIRNQDAMDKLR 869

Query: 23   EELR 12
             ELR
Sbjct: 870  GELR 873



 Score =  105 bits (263), Expect = 3e-21
 Identities = 61/125 (48%), Positives = 81/125 (64%), Gaps = 4/125 (3%)
 Frame = -3

Query: 377 LREKLFKTL--ENAV--APNISDFFPMLQRLDLQGLRKETLKHMKEIFSSWEGIINERRL 210
           +R  L K L  ENA   A NI DF+P+L  LD+QG+RK+  +  + I ++WE I+ ERR 
Sbjct: 207 VRGVLLKDLLNENAELGATNILDFYPILGGLDIQGIRKKLKEIFRRIPTTWEDILKERRK 266

Query: 209 VAVAKLEEQCFLDRLLENGFSNDQINTLALELFIAGTETTTLTIVWAMAELIKNKKVMVK 30
             +       FLD LLE GF +DQIN + +ELF AG ET++LT+ WAMAELIKN+  M K
Sbjct: 267 QRIHGSSHGDFLDALLETGFEDDQINHVIMELFFAGPETSSLTVEWAMAELIKNQDAMHK 326

Query: 29  VQEEL 15
           +  EL
Sbjct: 327 LCNEL 331



 Score =  103 bits (256), Expect = 2e-20
 Identities = 50/122 (40%), Positives = 79/122 (64%)
 Frame = -3

Query: 380  GLREKLFKTLENAVAPNISDFFPMLQRLDLQGLRKETLKHMKEIFSSWEGIINERRLVAV 201
            GL++ +++  E    PN+SDF+P+L  L+L G +K++ + +  I ++WEG + ERR    
Sbjct: 1251 GLKDFIYRAAELGATPNLSDFYPILDGLNLHGSKKKSKEALGRILATWEGTLKERRKQKN 1310

Query: 200  AKLEEQCFLDRLLENGFSNDQINTLALELFIAGTETTTLTIVWAMAELIKNKKVMVKVQE 21
                 +  L+  LE  F +DQIN + LELF AG +T+TLTI WA+ +LI+N  VM K+++
Sbjct: 1311 PGSSHRDLLEAFLEIRFEDDQINQVILELFSAGADTSTLTIEWAITQLIRNPDVMYKLRD 1370

Query: 20   EL 15
            EL
Sbjct: 1371 EL 1372


>ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
           [Vitis vinifera]
          Length = 498

 Score =  112 bits (281), Expect = 2e-23
 Identities = 55/124 (44%), Positives = 83/124 (66%)
 Frame = -3

Query: 383 GGLREKLFKTLENAVAPNISDFFPMLQRLDLQGLRKETLKHMKEIFSSWEGIINERRLVA 204
           G L++ + K  E    PN+SD++P+L  LD+QG+ ++  +  + I ++WE I+ ERR   
Sbjct: 210 GALKDLIHKNAEFGATPNLSDYYPILGGLDIQGINRKAKEMFERIPTTWEDILKERRTQR 269

Query: 203 VAKLEEQCFLDRLLENGFSNDQINTLALELFIAGTETTTLTIVWAMAELIKNKKVMVKVQ 24
             +   + FL+ LLE GF +DQIN + LELF AG ET++LT+ WAMAELI+N+  M K++
Sbjct: 270 SNRSSHRDFLEALLEIGFEDDQINQVILELFSAGAETSSLTVEWAMAELIRNQDAMDKLR 329

Query: 23  EELR 12
            ELR
Sbjct: 330 GELR 333