BLASTX nr result
ID: Atractylodes22_contig00031931
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Atractylodes22_contig00031931
(983 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002510043.1| Squalene monooxygenase, putative [Ricinus co... 504 e-152
ref|XP_004142907.1| PREDICTED: squalene monooxygenase-like [Cucu... 498 e-148
ref|XP_004159571.1| PREDICTED: squalene monooxygenase-like [Cucu... 498 e-148
gb|ADD17678.1| squalene epoxidase [Withania somnifera] 517 e-144
gb|AAY22200.1| squalene monooxygenase [Datura inoxia] 515 e-144
>ref|XP_002510043.1| Squalene monooxygenase, putative [Ricinus communis]
gi|223550744|gb|EEF52230.1| Squalene monooxygenase,
putative [Ricinus communis]
Length = 534
Score = 504 bits (1299), Expect(2) = e-152
Identities = 242/293 (82%), Positives = 267/293 (91%)
Frame = +2
Query: 104 RQKNGKEMKAYAPLTIVCDGCFSNLRRSLCNPQVDVPSCFVGLVLENCKLPYENHGHVVL 283
+ K G+E+ A+APLTIVCDGCFSNLRRSLCNP+VDVPSCFVGLVLE+CKLPY+ HGHVVL
Sbjct: 208 KTKTGEELTAFAPLTIVCDGCFSNLRRSLCNPKVDVPSCFVGLVLEDCKLPYQYHGHVVL 267
Query: 284 ADPSPILFYQISSTEIRCLVDVPGQKVPSILNGDLSNYLKTVVAPQIPVELQDAFIAAID 463
ADPSPILFYQISSTE+RCLVDVPGQKVPSI NG+++ YLK VVAPQ+P E+ D+F+AA+D
Sbjct: 268 ADPSPILFYQISSTEVRCLVDVPGQKVPSISNGEMAKYLKNVVAPQVPPEIYDSFVAAVD 327
Query: 464 KGNIRTMPNRSMPAAPHPTPGALLMGDAFNMRHPLTGGGMTVALSDIVVXXXXXXXXXXX 643
KGNIRTMPNRSMPA+P+PTPGALLMGDAFNMRHPLTGGGMTVALSDIVV
Sbjct: 328 KGNIRTMPNRSMPASPYPTPGALLMGDAFNMRHPLTGGGMTVALSDIVVLRELLKPLRDL 387
Query: 644 XXSYTLCKYLESFYTLRKPVASTINTLAGALYKVFCASPDKARQEMREACFDYLSLGGVF 823
+ TLC+YLESFYTLRKPVASTINTLAGALYKVFCAS D+AR EMR+ACFDYLSLGGVF
Sbjct: 388 HDAPTLCRYLESFYTLRKPVASTINTLAGALYKVFCASSDEARNEMRQACFDYLSLGGVF 447
Query: 824 SNGPISLLSGLNPRPLSLVIHFFAVAIYGVGRLLLPFPSPKRMWIGARLISGA 982
S GPISLLSGLNPRPLSLV+HFFAVAIYGVGRLLLPFPSPKR+W+GARLISGA
Sbjct: 448 STGPISLLSGLNPRPLSLVVHFFAVAIYGVGRLLLPFPSPKRVWVGARLISGA 500
Score = 62.4 bits (150), Expect(2) = e-152
Identities = 28/37 (75%), Positives = 35/37 (94%)
Frame = +1
Query: 1 KMREKASSLPNVRLKQGTVSSLLEEDGIIKGAIYKTK 111
+MREKASS+PNVRL+QGTV+SL+EE GII+G +YKTK
Sbjct: 174 RMREKASSIPNVRLEQGTVTSLIEEKGIIRGVVYKTK 210
>ref|XP_004142907.1| PREDICTED: squalene monooxygenase-like [Cucumis sativus]
Length = 528
Score = 498 bits (1281), Expect(2) = e-148
Identities = 242/293 (82%), Positives = 261/293 (89%)
Frame = +2
Query: 104 RQKNGKEMKAYAPLTIVCDGCFSNLRRSLCNPQVDVPSCFVGLVLENCKLPYENHGHVVL 283
+ KNG++ AYAPLTIVCDGCFSNLRRSLCNP VDVPSCFVGLVLENC+LPY N GHVVL
Sbjct: 202 KSKNGEQKTAYAPLTIVCDGCFSNLRRSLCNPMVDVPSCFVGLVLENCQLPYANLGHVVL 261
Query: 284 ADPSPILFYQISSTEIRCLVDVPGQKVPSILNGDLSNYLKTVVAPQIPVELQDAFIAAID 463
DPSPILFY ISSTEIRCLVDVPGQKVPSI NG++ YLKTVVAPQ+P ++ DAFIAAI+
Sbjct: 262 GDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYLKTVVAPQVPPQIHDAFIAAIE 321
Query: 464 KGNIRTMPNRSMPAAPHPTPGALLMGDAFNMRHPLTGGGMTVALSDIVVXXXXXXXXXXX 643
KGNIRTMPNRSMPAAP PTPGALLMGDAFNMRHPLTGGGMTVALSDIVV
Sbjct: 322 KGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGGMTVALSDIVVLRNLLKPLKDL 381
Query: 644 XXSYTLCKYLESFYTLRKPVASTINTLAGALYKVFCASPDKARQEMREACFDYLSLGGVF 823
+ TLCKYLESFYTLRKPVASTINTLAGALYKVFCAS D+AR+EMR+ACFDYLSLGG+F
Sbjct: 382 NDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASSDQARKEMRQACFDYLSLGGIF 441
Query: 824 SNGPISLLSGLNPRPLSLVIHFFAVAIYGVGRLLLPFPSPKRMWIGARLISGA 982
SNGP+SLLSGLNPRPLSLV+HFFAVAIYGVGRLLLPFPSPK +WIGARL+ A
Sbjct: 442 SNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPSPKGIWIGARLVYSA 494
Score = 53.9 bits (128), Expect(2) = e-148
Identities = 26/41 (63%), Positives = 33/41 (80%)
Frame = +1
Query: 1 KMREKASSLPNVRLKQGTVSSLLEEDGIIKGAIYKTKKWQR 123
+MREKA+ LPNVRL+QGTV+SLLEE G I G YK+K ++
Sbjct: 168 RMREKAAFLPNVRLEQGTVTSLLEEKGTITGVQYKSKNGEQ 208
>ref|XP_004159571.1| PREDICTED: squalene monooxygenase-like [Cucumis sativus]
Length = 469
Score = 498 bits (1281), Expect(2) = e-148
Identities = 242/293 (82%), Positives = 261/293 (89%)
Frame = +2
Query: 104 RQKNGKEMKAYAPLTIVCDGCFSNLRRSLCNPQVDVPSCFVGLVLENCKLPYENHGHVVL 283
+ KNG++ AYAPLTIVCDGCFSNLRRSLCNP VDVPSCFVGLVLENC+LPY N GHVVL
Sbjct: 143 KSKNGEQKTAYAPLTIVCDGCFSNLRRSLCNPMVDVPSCFVGLVLENCQLPYANLGHVVL 202
Query: 284 ADPSPILFYQISSTEIRCLVDVPGQKVPSILNGDLSNYLKTVVAPQIPVELQDAFIAAID 463
DPSPILFY ISSTEIRCLVDVPGQKVPSI NG++ YLKTVVAPQ+P ++ DAFIAAI+
Sbjct: 203 GDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYLKTVVAPQVPPQIHDAFIAAIE 262
Query: 464 KGNIRTMPNRSMPAAPHPTPGALLMGDAFNMRHPLTGGGMTVALSDIVVXXXXXXXXXXX 643
KGNIRTMPNRSMPAAP PTPGALLMGDAFNMRHPLTGGGMTVALSDIVV
Sbjct: 263 KGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGGMTVALSDIVVLRNLLKPLKDL 322
Query: 644 XXSYTLCKYLESFYTLRKPVASTINTLAGALYKVFCASPDKARQEMREACFDYLSLGGVF 823
+ TLCKYLESFYTLRKPVASTINTLAGALYKVFCAS D+AR+EMR+ACFDYLSLGG+F
Sbjct: 323 NDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASSDQARKEMRQACFDYLSLGGIF 382
Query: 824 SNGPISLLSGLNPRPLSLVIHFFAVAIYGVGRLLLPFPSPKRMWIGARLISGA 982
SNGP+SLLSGLNPRPLSLV+HFFAVAIYGVGRLLLPFPSPK +WIGARL+ A
Sbjct: 383 SNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPSPKGIWIGARLVYSA 435
Score = 53.9 bits (128), Expect(2) = e-148
Identities = 26/41 (63%), Positives = 33/41 (80%)
Frame = +1
Query: 1 KMREKASSLPNVRLKQGTVSSLLEEDGIIKGAIYKTKKWQR 123
+MREKA+ LPNVRL+QGTV+SLLEE G I G YK+K ++
Sbjct: 109 RMREKAAFLPNVRLEQGTVTSLLEEKGTITGVQYKSKNGEQ 149
>gb|ADD17678.1| squalene epoxidase [Withania somnifera]
Length = 531
Score = 517 bits (1332), Expect = e-144
Identities = 248/305 (81%), Positives = 280/305 (91%)
Frame = +2
Query: 68 LKKTASLKVLFTRQKNGKEMKAYAPLTIVCDGCFSNLRRSLCNPQVDVPSCFVGLVLENC 247
L++ +++ + + K+G+E+KAYAPLTIVCDGCFSNLRR+LC+P+V+VPSCFVGLVLENC
Sbjct: 193 LEENGTIRGVQYKTKSGEELKAYAPLTIVCDGCFSNLRRTLCDPKVEVPSCFVGLVLENC 252
Query: 248 KLPYENHGHVVLADPSPILFYQISSTEIRCLVDVPGQKVPSILNGDLSNYLKTVVAPQIP 427
+LP+ENHGHV+LADPSPILFY ISSTE+RCLVDVPGQKVPSI NG+++ YLKTVVAPQ+P
Sbjct: 253 QLPHENHGHVILADPSPILFYPISSTEVRCLVDVPGQKVPSISNGEMAKYLKTVVAPQVP 312
Query: 428 VELQDAFIAAIDKGNIRTMPNRSMPAAPHPTPGALLMGDAFNMRHPLTGGGMTVALSDIV 607
E++DAFIAA+DKGNIRTMPNRSMPAAPHPTPGALLMGDAFNMRHPLTGGGMTVALSDIV
Sbjct: 313 SEIKDAFIAAVDKGNIRTMPNRSMPAAPHPTPGALLMGDAFNMRHPLTGGGMTVALSDIV 372
Query: 608 VXXXXXXXXXXXXXSYTLCKYLESFYTLRKPVASTINTLAGALYKVFCASPDKARQEMRE 787
V + TLC+YLESFYTLRKPVASTINTLAGALYKVFCASPD+AR+EMRE
Sbjct: 373 VLRDLLKPLRDLNDAPTLCRYLESFYTLRKPVASTINTLAGALYKVFCASPDQARKEMRE 432
Query: 788 ACFDYLSLGGVFSNGPISLLSGLNPRPLSLVIHFFAVAIYGVGRLLLPFPSPKRMWIGAR 967
ACFDYLSLGGVFS+GP+SLLSGLNPRPLSLV HFFAVAIYGVGRLLLPFPSPKR+WIGAR
Sbjct: 433 ACFDYLSLGGVFSSGPVSLLSGLNPRPLSLVCHFFAVAIYGVGRLLLPFPSPKRLWIGAR 492
Query: 968 LISGA 982
LISGA
Sbjct: 493 LISGA 497
Score = 58.2 bits (139), Expect = 3e-06
Identities = 27/37 (72%), Positives = 34/37 (91%)
Frame = +1
Query: 1 KMREKASSLPNVRLKQGTVSSLLEEDGIIKGAIYKTK 111
+MREKA+SLPNV+L+QGTV+SLLEE+G I+G YKTK
Sbjct: 171 RMREKAASLPNVKLEQGTVTSLLEENGTIRGVQYKTK 207
>gb|AAY22200.1| squalene monooxygenase [Datura inoxia]
Length = 529
Score = 515 bits (1326), Expect = e-144
Identities = 247/305 (80%), Positives = 278/305 (91%)
Frame = +2
Query: 68 LKKTASLKVLFTRQKNGKEMKAYAPLTIVCDGCFSNLRRSLCNPQVDVPSCFVGLVLENC 247
L++ ++K + + K+G+E+KAYAPLT+VCDGCFSNLRR+LC+P+V+VPSCFVGLVLENC
Sbjct: 191 LEENGTIKGVQYKTKSGEELKAYAPLTVVCDGCFSNLRRTLCDPKVEVPSCFVGLVLENC 250
Query: 248 KLPYENHGHVVLADPSPILFYQISSTEIRCLVDVPGQKVPSILNGDLSNYLKTVVAPQIP 427
+LP+ENHGHV+LADPSPILFY ISSTE+RCLVDVPGQKVPSI NG+++ YLK VVAPQ+P
Sbjct: 251 QLPHENHGHVILADPSPILFYPISSTEVRCLVDVPGQKVPSISNGEMAKYLKNVVAPQVP 310
Query: 428 VELQDAFIAAIDKGNIRTMPNRSMPAAPHPTPGALLMGDAFNMRHPLTGGGMTVALSDIV 607
E++DAFIAAI+KGNIRTMPNRSMPAAPHPTPGALLMGDAFNMRHPLTGGGMTVALSDIV
Sbjct: 311 AEIKDAFIAAINKGNIRTMPNRSMPAAPHPTPGALLMGDAFNMRHPLTGGGMTVALSDIV 370
Query: 608 VXXXXXXXXXXXXXSYTLCKYLESFYTLRKPVASTINTLAGALYKVFCASPDKARQEMRE 787
V + TLC+YLESFYTLRKPVASTINTLAGALYKVFCASPD+AR+EMRE
Sbjct: 371 VLRDLLKPLHDLNDAPTLCRYLESFYTLRKPVASTINTLAGALYKVFCASPDQARKEMRE 430
Query: 788 ACFDYLSLGGVFSNGPISLLSGLNPRPLSLVIHFFAVAIYGVGRLLLPFPSPKRMWIGAR 967
ACFDYLSLGGVFS GP+SLLSGLNPRPLSLV HFFAVAIYGVGRLLLPFPSPKR+WIGAR
Sbjct: 431 ACFDYLSLGGVFSEGPVSLLSGLNPRPLSLVCHFFAVAIYGVGRLLLPFPSPKRLWIGAR 490
Query: 968 LISGA 982
LISGA
Sbjct: 491 LISGA 495
Score = 59.3 bits (142), Expect = 1e-06
Identities = 28/37 (75%), Positives = 34/37 (91%)
Frame = +1
Query: 1 KMREKASSLPNVRLKQGTVSSLLEEDGIIKGAIYKTK 111
+MREKA+SLPNV+L+QGTV+SLLEE+G IKG YKTK
Sbjct: 169 RMREKAASLPNVKLEQGTVTSLLEENGTIKGVQYKTK 205