BLASTX nr result
ID: Atractylodes22_contig00031203
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Atractylodes22_contig00031203
(995 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|D5JBX1.1|GAO_BARSP RecName: Full=Germacrene A oxidase; Short=... 340 3e-91
gb|ADM86719.1| valencene oxidase [Cichorium intybus] 339 5e-91
ref|XP_003529672.1| PREDICTED: cytochrome P450 71D8-like [Glycin... 338 2e-90
sp|A6YIH8.1|C7D55_HYOMU RecName: Full=Premnaspirodiene oxygenase... 335 8e-90
ref|XP_002264048.2| PREDICTED: cytochrome P450 71D11-like [Vitis... 334 2e-89
>sp|D5JBX1.1|GAO_BARSP RecName: Full=Germacrene A oxidase; Short=BsGAO; AltName:
Full=Germacrene A alcohol dehydrogenase; AltName:
Full=Germacrene A hydroxylase gi|294845888|gb|ADF43083.1|
germacrene A oxidase [Barnadesia spinosa]
Length = 496
Score = 340 bits (872), Expect = 3e-91
Identities = 172/326 (52%), Positives = 220/326 (67%)
Frame = -2
Query: 988 IDLSEKIFTMMNGIICKVAIGGRCKDQATLLALIDEIISLCGGFDVSDLFPSIKLLHLVT 809
+DLSE IF ++ I+ + A G KDQ ++ EI+ L GGFDV+D+FPS K+LH ++
Sbjct: 168 VDLSESIFKLIATILSRAAFGKGIKDQREFTEIVKEILRLTGGFDVADIFPSKKILHHLS 227
Query: 808 GMRKKLVKIHNKIDSILYDIISDHQHRAGRPSNHNEXXXXXXXXXXXXXXLQFPLTSDNI 629
G R KL IHNK+DS++ +I+S+H R S+ E + PLTSDN+
Sbjct: 228 GKRAKLTNIHNKLDSLINNIVSEHP--GSRTSSSQESLLDVLLRLKDSA--ELPLTSDNV 283
Query: 628 KAVILEMFAAGTDTSSVTIDWAMSELMKNPRVMKKAQDELRKTLKGKKKIHESDIQELDY 449
KAVIL+MF AGTDTSS TI+WA+SEL++ PR M+K Q ELR+ L GK++I E DIQEL Y
Sbjct: 284 KAVILDMFGAGTDTSSATIEWAISELIRCPRAMEKVQTELRQALNGKERIQEEDIQELSY 343
Query: 448 LKLVIKETXXXXXXXXXXXXRECREKCEIGGYHIPVDTKVIINSWEIGRDPNYWIDPEIF 269
LKLVIKET RECRE C + GY IP TK+I+N + I RDP YW D E F
Sbjct: 344 LKLVIKETLRLHPPLPLVMPRECREPCVLAGYEIPTKTKLIVNVFAINRDPEYWKDAETF 403
Query: 268 MPERFSESSIKMTGMDFEYLPFGGGRRICPGMNLGLANVELPLAMLLYHFDWELPTEVTS 89
MPERF S I + G ++EYLPFG GRR+CPG LGLANVELPLA +LY+F+W+LP
Sbjct: 404 MPERFENSPINIMGSEYEYLPFGAGRRMCPGAALGLANVELPLAHILYYFNWKLPNGARL 463
Query: 88 ENLDMSEVFGGTLKRKNNLHLVPSVY 11
+ LDMSE FG T++RK+ L LVP+ Y
Sbjct: 464 DELDMSECFGATVQRKSELLLVPTAY 489
>gb|ADM86719.1| valencene oxidase [Cichorium intybus]
Length = 496
Score = 339 bits (870), Expect = 5e-91
Identities = 174/324 (53%), Positives = 223/324 (68%)
Frame = -2
Query: 988 IDLSEKIFTMMNGIICKVAIGGRCKDQATLLALIDEIISLCGGFDVSDLFPSIKLLHLVT 809
I+LSE IF M+ I+ + A G KDQ L+ EI+ L GGFDV+D+FPS KLLH ++
Sbjct: 168 INLSENIFKMIATILSRAAFGKGIKDQMKFTELVKEILRLTGGFDVADIFPSKKLLHHLS 227
Query: 808 GMRKKLVKIHNKIDSILYDIISDHQHRAGRPSNHNEXXXXXXXXXXXXXXLQFPLTSDNI 629
G R KL IHNK+D+++ +II++H R S+ E +FPLT+DN+
Sbjct: 228 GKRAKLTNIHNKLDNLINNIIAEHP--GNRTSSSQETLLDVLLRLKESA--EFPLTADNV 283
Query: 628 KAVILEMFAAGTDTSSVTIDWAMSELMKNPRVMKKAQDELRKTLKGKKKIHESDIQELDY 449
KAVIL+MF AGTDTSS TI+WA+SEL++ PR M+K Q ELR+ L GK++I E D+QEL+Y
Sbjct: 284 KAVILDMFGAGTDTSSATIEWAISELIRCPRAMEKVQTELRQALNGKERIQEEDLQELNY 343
Query: 448 LKLVIKETXXXXXXXXXXXXRECREKCEIGGYHIPVDTKVIINSWEIGRDPNYWIDPEIF 269
LKLVIKET RECRE C +GGY IP TK+I+N + I RDP YW D E F
Sbjct: 344 LKLVIKETLRLHPPLPLVMPRECREPCVLGGYDIPSKTKLIVNVFAINRDPEYWKDAETF 403
Query: 268 MPERFSESSIKMTGMDFEYLPFGGGRRICPGMNLGLANVELPLAMLLYHFDWELPTEVTS 89
MPERF S I + G ++EYLPFG GRR+CPG LGLANVELPLA +LY+F+W+LP T
Sbjct: 404 MPERFENSPITVMGSEYEYLPFGAGRRMCPGAALGLANVELPLAHILYYFNWKLPNGKTF 463
Query: 88 ENLDMSEVFGGTLKRKNNLHLVPS 17
E+LDM+E FG T++RK L LVP+
Sbjct: 464 EDLDMTESFGATVQRKTELLLVPT 487
>ref|XP_003529672.1| PREDICTED: cytochrome P450 71D8-like [Glycine max]
Length = 511
Score = 338 bits (866), Expect = 2e-90
Identities = 166/327 (50%), Positives = 225/327 (68%)
Frame = -2
Query: 988 IDLSEKIFTMMNGIICKVAIGGRCKDQATLLALIDEIISLCGGFDVSDLFPSIKLLHLVT 809
+++S+ +F +++ +I + A G + + + LLAL+ + + L GGFD++DLFPS+K +HL+T
Sbjct: 177 VNVSKSVFFLLSTLISRAAFGKKSEYEDKLLALLKKAVELTGGFDLADLFPSMKPIHLIT 236
Query: 808 GMRKKLVKIHNKIDSILYDIISDHQHRAGRPSNHNEXXXXXXXXXXXXXXLQFPLTSDNI 629
M+ KL + ++D IL +II+ HQ G+ E L+ +T +NI
Sbjct: 237 RMKAKLEDMQKELDKILENIINQHQSNHGK-GEAEENLVDVLLRVQKSGSLEIQVTINNI 295
Query: 628 KAVILEMFAAGTDTSSVTIDWAMSELMKNPRVMKKAQDELRKTLKGKKKIHESDIQELDY 449
KAVI ++F AGTDTS+ ++WAMSELMKNPRVMKKAQ E+R+ +GKK I ESD+ EL Y
Sbjct: 296 KAVIWDIFGAGTDTSATVLEWAMSELMKNPRVMKKAQAEIREAFRGKKTIRESDVYELSY 355
Query: 448 LKLVIKETXXXXXXXXXXXXRECREKCEIGGYHIPVDTKVIINSWEIGRDPNYWIDPEIF 269
LK VIKET RECRE C+IGGY IP+ TKVI+N+W +GRDP +W D E F
Sbjct: 356 LKSVIKETMRLHPPVPLLLPRECREPCKIGGYEIPIKTKVIVNAWALGRDPKHWYDAEKF 415
Query: 268 MPERFSESSIKMTGMDFEYLPFGGGRRICPGMNLGLANVELPLAMLLYHFDWELPTEVTS 89
+PERF +S G +FEY+PFG GRR+CPG+ LG+ANVELPL LLYHFDWELP +
Sbjct: 416 IPERFDGTSNDFKGSNFEYIPFGAGRRMCPGILLGIANVELPLVALLYHFDWELPNGMKP 475
Query: 88 ENLDMSEVFGGTLKRKNNLHLVPSVYD 8
E+LDM+E FG + RKNNL+L+PS YD
Sbjct: 476 EDLDMTEGFGAAVGRKNNLYLMPSPYD 502
>sp|A6YIH8.1|C7D55_HYOMU RecName: Full=Premnaspirodiene oxygenase; Short=HPO; AltName:
Full=Cytochrome P450 71D55 gi|151335776|gb|ABS00393.1|
cytochrome P450 hydroxylase [Hyoscyamus muticus]
Length = 502
Score = 335 bits (860), Expect = 8e-90
Identities = 162/330 (49%), Positives = 226/330 (68%), Gaps = 4/330 (1%)
Frame = -2
Query: 988 IDLSEKIFTMMNGIICKVAIGGRCKDQATLLALIDEIISLCGGFDVSDLFPSIKLLHLVT 809
++ +E++F + + C+ A G K+Q T + LI E+I L GGFDV+D+FPS+K LH++T
Sbjct: 168 VNFTERLFLFTSSMTCRSAFGKVFKEQETFIQLIKEVIGLAGGFDVADIFPSLKFLHVLT 227
Query: 808 GMRKKLVKIHNKIDSILYDIISDHQHRAGRPSNHN----EXXXXXXXXXXXXXXLQFPLT 641
GM K++K H+K+D+I+ D+I++H+ + E LQFP+T
Sbjct: 228 GMEGKIMKAHHKVDAIVEDVINEHKKNLAMGKTNGALGGEDLIDVLLRLMNDGGLQFPIT 287
Query: 640 SDNIKAVILEMFAAGTDTSSVTIDWAMSELMKNPRVMKKAQDELRKTLKGKKKIHESDIQ 461
+DNIKA+I +MFAAGT+TSS T+ WAM ++M+NP ++ KAQ E+R+ KGK+ E+D++
Sbjct: 288 NDNIKAIIFDMFAAGTETSSSTLVWAMVQMMRNPTILAKAQAEVREAFKGKETFDENDVE 347
Query: 460 ELDYLKLVIKETXXXXXXXXXXXXRECREKCEIGGYHIPVDTKVIINSWEIGRDPNYWID 281
EL YLKLVIKET RECRE+ EI GY IPV TKV++N W +GRDP YW D
Sbjct: 348 ELKYLKLVIKETLRLHPPVPLLVPRECREETEINGYTIPVKTKVMVNVWALGRDPKYWDD 407
Query: 280 PEIFMPERFSESSIKMTGMDFEYLPFGGGRRICPGMNLGLANVELPLAMLLYHFDWELPT 101
+ F PERF + S+ G +FEYLPFGGGRRICPG++ GLANV LPLA LLYHFDW+LPT
Sbjct: 408 ADNFKPERFEQCSVDFIGNNFEYLPFGGGRRICPGISFGLANVYLPLAQLLYHFDWKLPT 467
Query: 100 EVTSENLDMSEVFGGTLKRKNNLHLVPSVY 11
+ ++LD++E+ G T RK++L LV + Y
Sbjct: 468 GMEPKDLDLTELVGVTAARKSDLMLVATPY 497
>ref|XP_002264048.2| PREDICTED: cytochrome P450 71D11-like [Vitis vinifera]
Length = 485
Score = 334 bits (857), Expect = 2e-89
Identities = 166/331 (50%), Positives = 226/331 (68%), Gaps = 5/331 (1%)
Frame = -2
Query: 988 IDLSEKIFTMMNGIICKVAIGGRCKDQATLLALIDEIISLCGGFDVSDLFPSIKLLHLVT 809
I+L+EK F + I + A G +CK Q T ++++ E I L GGF+V D+FPS K LHL++
Sbjct: 149 INLTEKTFASICAITTRAAFGKKCKYQETFISVLLETIKLAGGFNVGDIFPSFKSLHLIS 208
Query: 808 GMRKKLVKIHNKIDSILYDIISDHQHRAG-----RPSNHNEXXXXXXXXXXXXXXLQFPL 644
GMR KL K+H + D IL +II +H+ R G + + F L
Sbjct: 209 GMRPKLEKLHQEADKILENIIHEHKARGGTTKIDKDGPDEDLVDVLLKFHEDHGDHAFSL 268
Query: 643 TSDNIKAVILEMFAAGTDTSSVTIDWAMSELMKNPRVMKKAQDELRKTLKGKKKIHESDI 464
T+DNIKAV+L++F AG++ SS TID+AMSE+M+NPR+M+KAQ+E+R+ K++I E I
Sbjct: 269 TTDNIKAVLLDIFGAGSEPSSTTIDFAMSEMMRNPRIMRKAQEEVRRIFDRKEEIDEMGI 328
Query: 463 QELDYLKLVIKETXXXXXXXXXXXXRECREKCEIGGYHIPVDTKVIINSWEIGRDPNYWI 284
QEL +LKLVIKET RECREKCEI G+ IPV +K+I+N+W IGRDP +W
Sbjct: 329 QELKFLKLVIKETLRLHPPLPLLLPRECREKCEIDGHEIPVKSKIIVNAWAIGRDPKHWT 388
Query: 283 DPEIFMPERFSESSIKMTGMDFEYLPFGGGRRICPGMNLGLANVELPLAMLLYHFDWELP 104
+PE F PERF +SSI G +FEY+PFG GRRICPG+ GLA+VEL LA LLYHFDW+LP
Sbjct: 389 EPESFNPERFLDSSIDYKGTNFEYIPFGAGRRICPGILFGLASVELLLAKLLYHFDWKLP 448
Query: 103 TEVTSENLDMSEVFGGTLKRKNNLHLVPSVY 11
+ ++LDM+EVFG ++RK +L+L+P+ Y
Sbjct: 449 NGMKQQDLDMTEVFGLAVRRKEDLYLIPTAY 479