BLASTX nr result
ID: Atractylodes22_contig00022788
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Atractylodes22_contig00022788
(1064 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|ABD39696.1| littorine mutase/monooxygenase [Hyoscyamus niger] 257 4e-66
emb|CBI31830.3| unnamed protein product [Vitis vinifera] 235 1e-59
ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 235 1e-59
ref|XP_002318833.1| cytochrome P450 [Populus trichocarpa] gi|222... 235 2e-59
emb|CAN62647.1| hypothetical protein VITISV_013219 [Vitis vinifera] 235 2e-59
>gb|ABD39696.1| littorine mutase/monooxygenase [Hyoscyamus niger]
Length = 510
Score = 257 bits (656), Expect = 4e-66
Identities = 138/288 (47%), Positives = 199/288 (69%), Gaps = 7/288 (2%)
Frame = +1
Query: 220 IVGNLFQLGENNLHIRLAEMAQVHGP-LMSLRLGQQILIVGSSATAASEILKTHDHVLSG 396
I+G+LF LG N H+ LA++A VHGP LMS+RLG +++IV SS A +E+LKTHD +LSG
Sbjct: 48 IIGHLFYLG-NKPHVSLAKLANVHGPHLMSIRLGGRLVIVASSPMATAEVLKTHDRLLSG 106
Query: 397 RPVSQLLQRKVPTAHNRNLVLTS--ECNDDWRMLRNIYRSELFSRKALESSAKMRENKVM 570
R VS + +V ++ RNL + EC+++W+ +R++Y+ LFS KA+ES +RE KVM
Sbjct: 107 RFVSHPM--RVEGSYIRNLATETLEECDENWKKVRSMYQIVLFSHKAVESQVNIREKKVM 164
Query: 571 EMVKYLGSKEGEDIIIKNVAFANSVNILGNASLSIDLVDFEGNGIGEGIMDSVKRLNKLA 750
E+VK++ SKEGE + IK +AF +NIL N+++S DLVDFEG GIGEG+ + ++ KL
Sbjct: 165 ELVKFVASKEGELVNIKGIAFVTILNILSNSTISNDLVDFEGKGIGEGMREWIRNYTKLE 224
Query: 751 AKPILADMYPILS--RWDLQGWYKQLMHIIEREFGGVWEDSLKMKRNIV--EFLGPKDFV 918
P LAD++PIL WD QG YK+L ER VW D + KR + ++ +DF
Sbjct: 225 GVPQLADLFPILDGCTWDFQGTYKKLKDTFER-ISDVWRDIINKKRMEISNKYYEGEDFA 283
Query: 919 DILIEKGFTNQQINPIMEELFAAGTESTSASIEWLMVELLRNPEVMQK 1062
D LI GF ++QIN ++ EL++AGTE+T ++EW +VELL+NPE M++
Sbjct: 284 DALIRNGFEDKQINALLMELYSAGTETTITTVEWTLVELLKNPEAMKR 331
>emb|CBI31830.3| unnamed protein product [Vitis vinifera]
Length = 1538
Score = 235 bits (600), Expect = 1e-59
Identities = 129/304 (42%), Positives = 187/304 (61%), Gaps = 2/304 (0%)
Frame = +1
Query: 157 MITKHRVSKELXXXXXXXXXXIVGNLFQLGENNLHIRLAEMAQVHGPLMSLRLGQQILIV 336
+I KH K I+GNL Q+G+N H +LA +A++HGPLMSLRLG Q+++V
Sbjct: 566 VILKHIFLKPPPLPPGPYPWPIIGNLLQMGKNP-HAKLANLAKLHGPLMSLRLGTQLMVV 624
Query: 337 GSSATAASEILKTHDHVLSGRPVSQLLQRKVPTAHNRNLVLTSECNDDWRMLRNIYRSEL 516
SS AA E+LKTHD LSGR +S + K P ++ ++V +CN +W+ LR I R EL
Sbjct: 625 ASSPAAAMEVLKTHDRALSGRYLSSSVPVKNPKLNHLSIVFAKDCNTNWKNLRAICRMEL 684
Query: 517 FSRKALESSAKMRENKVMEMVKYLGSKEGEDIIIKNVAFANSVNILGNASLSIDLVDFEG 696
FS KA+ES ++RE KV E+V++L +KEGE + + ++ F NIL N S+DL DFE
Sbjct: 685 FSGKAMESQVELRERKVTELVEFLATKEGEVVKVMDLVFTTICNILSNKFFSMDLCDFED 744
Query: 697 NG-IGEGIMDSVKRLNKLAAKPILADMYPILSRWDLQGWYKQLMHIIEREFGGVWEDSLK 873
G +G + D + + + A P L+D YPIL D+QG ++ + ER WED LK
Sbjct: 745 EGRVGGALKDLIHKNAEFGATPNLSDYYPILGGLDIQGINRKAKEMFER-IPTTWEDILK 803
Query: 874 MKR-NIVEFLGPKDFVDILIEKGFTNQQINPIMEELFAAGTESTSASIEWLMVELLRNPE 1050
+R +DF++ L+E GF + QIN ++ ELF+AG E++S ++EW M EL+RN +
Sbjct: 804 ERRTQRSNRSSHRDFLEALLEIGFEDDQINQVILELFSAGAETSSLTVEWAMAELIRNQD 863
Query: 1051 VMQK 1062
M K
Sbjct: 864 AMDK 867
Score = 232 bits (591), Expect = 1e-58
Identities = 134/304 (44%), Positives = 181/304 (59%), Gaps = 2/304 (0%)
Frame = +1
Query: 157 MITKHRVSKELXXXXXXXXXXIVGNLFQLGENNLHIRLAEMAQVHGPLMSLRLGQQILIV 336
+I KH K I+GNL Q+G NLH++LA +A+ HGPLMSLRLG QI++V
Sbjct: 26 LIFKHIFLKSPPLPPGPYPWPIIGNLLQMG-GNLHVKLANLAKRHGPLMSLRLGTQIMVV 84
Query: 337 GSSATAASEILKTHDHVLSGRPVSQLLQRKVPTAHNRNLVLTSECNDDWRMLRNIYRSEL 516
SS+ AA E+LKTHD LSGR VS + P ++ + CN DWR L+ I R EL
Sbjct: 85 ASSSAAAMEVLKTHDRTLSGRYVSTTIPVNSPKLNHLAMAFAKVCNSDWRNLKAICRMEL 144
Query: 517 FSRKALESSAKMRENKVMEMVKYLGSKEGEDIIIKNVAFANSVNILGNASLSIDLVDFEG 696
FS KA+ES ++RE KVME+V++L KEGE + + ++ + NIL N SID DFEG
Sbjct: 145 FSGKAMESRVELRERKVMELVEFLEKKEGEVVKVMDLVYTTVCNILSNKFFSIDFSDFEG 204
Query: 697 NGI-GEGIMDSVKRLNKLAAKPILADMYPILSRWDLQGWYKQLMHIIEREFGGVWEDSLK 873
+ G + D + +L A IL D YPIL D+QG K+L I R WED LK
Sbjct: 205 RDVRGVLLKDLLNENAELGATNIL-DFYPILGGLDIQGIRKKLKEIF-RRIPTTWEDILK 262
Query: 874 MKR-NIVEFLGPKDFVDILIEKGFTNQQINPIMEELFAAGTESTSASIEWLMVELLRNPE 1050
+R + DF+D L+E GF + QIN ++ ELF AG E++S ++EW M EL++N +
Sbjct: 263 ERRKQRIHGSSHGDFLDALLETGFEDDQINHVIMELFFAGPETSSLTVEWAMAELIKNQD 322
Query: 1051 VMQK 1062
M K
Sbjct: 323 AMHK 326
Score = 230 bits (586), Expect = 5e-58
Identities = 128/303 (42%), Positives = 186/303 (61%), Gaps = 1/303 (0%)
Frame = +1
Query: 157 MITKHRVSKELXXXXXXXXXXIVGNLFQLGENNLHIRLAEMAQVHGPLMSLRLGQQILIV 336
+I KH K I+GNL QLG+N H++LA +A++HGPLMSLRLG Q+++V
Sbjct: 1071 LILKHIFLKPPPLPPGPYPWPIIGNLLQLGKNP-HVKLASLAKLHGPLMSLRLGTQLMVV 1129
Query: 337 GSSATAASEILKTHDHVLSGRPVSQLLQRKVPTAHNRNLVLTSECNDDWRMLRNIYRSEL 516
SS AA E+LKTHD LSGR VS L K P ++ +L EC ++W+ LR I R+E+
Sbjct: 1130 ASSPAAALEVLKTHDRTLSGRYVSSSLSVKDPKLNHLSLAFAKECTNNWKNLRTICRTEM 1189
Query: 517 FSRKALESSAKMRENKVMEMVKYLGSKEGEDIIIKNVAFANSVNILGNASLSIDLVDFEG 696
FS KA+ES ++RE KVME+V++L +KEGE + + ++ F NIL N S+DL DFE
Sbjct: 1190 FSGKAMESHVELRERKVMELVEFLATKEGEVVKVMDLVFTTICNILSNTFFSMDLCDFE- 1248
Query: 697 NGIGEGIMDSVKRLNKLAAKPILADMYPILSRWDLQGWYKQLMHIIEREFGGVWEDSLKM 876
EG+ D + R +L A P L+D YPIL +L G K+ + R WE +LK
Sbjct: 1249 ---REGLKDFIYRAAELGATPNLSDFYPILDGLNLHGSKKKSKEALGRIL-ATWEGTLKE 1304
Query: 877 KRNIVE-FLGPKDFVDILIEKGFTNQQINPIMEELFAAGTESTSASIEWLMVELLRNPEV 1053
+R +D ++ +E F + QIN ++ ELF+AG ++++ +IEW + +L+RNP+V
Sbjct: 1305 RRKQKNPGSSHRDLLEAFLEIRFEDDQINQVILELFSAGADTSTLTIEWAITQLIRNPDV 1364
Query: 1054 MQK 1062
M K
Sbjct: 1365 MYK 1367
>ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Vitis vinifera]
Length = 498
Score = 235 bits (600), Expect = 1e-59
Identities = 129/304 (42%), Positives = 187/304 (61%), Gaps = 2/304 (0%)
Frame = +1
Query: 157 MITKHRVSKELXXXXXXXXXXIVGNLFQLGENNLHIRLAEMAQVHGPLMSLRLGQQILIV 336
+I KH K I+GNL Q+G+N H +LA +A++HGPLMSLRLG Q+++V
Sbjct: 26 VILKHIFLKPPPLPPGPYPWPIIGNLLQMGKNP-HAKLANLAKLHGPLMSLRLGTQLMVV 84
Query: 337 GSSATAASEILKTHDHVLSGRPVSQLLQRKVPTAHNRNLVLTSECNDDWRMLRNIYRSEL 516
SS AA E+LKTHD LSGR +S + K P ++ ++V +CN +W+ LR I R EL
Sbjct: 85 ASSPAAAMEVLKTHDRALSGRYLSSSVPVKNPKLNHLSIVFAKDCNTNWKNLRAICRMEL 144
Query: 517 FSRKALESSAKMRENKVMEMVKYLGSKEGEDIIIKNVAFANSVNILGNASLSIDLVDFEG 696
FS KA+ES ++RE KV E+V++L +KEGE + + ++ F NIL N S+DL DFE
Sbjct: 145 FSGKAMESQVELRERKVTELVEFLATKEGEVVKVMDLVFTTICNILSNKFFSMDLCDFED 204
Query: 697 NG-IGEGIMDSVKRLNKLAAKPILADMYPILSRWDLQGWYKQLMHIIEREFGGVWEDSLK 873
G +G + D + + + A P L+D YPIL D+QG ++ + ER WED LK
Sbjct: 205 EGRVGGALKDLIHKNAEFGATPNLSDYYPILGGLDIQGINRKAKEMFER-IPTTWEDILK 263
Query: 874 MKR-NIVEFLGPKDFVDILIEKGFTNQQINPIMEELFAAGTESTSASIEWLMVELLRNPE 1050
+R +DF++ L+E GF + QIN ++ ELF+AG E++S ++EW M EL+RN +
Sbjct: 264 ERRTQRSNRSSHRDFLEALLEIGFEDDQINQVILELFSAGAETSSLTVEWAMAELIRNQD 323
Query: 1051 VMQK 1062
M K
Sbjct: 324 AMDK 327
>ref|XP_002318833.1| cytochrome P450 [Populus trichocarpa] gi|222859506|gb|EEE97053.1|
cytochrome P450 [Populus trichocarpa]
Length = 503
Score = 235 bits (599), Expect = 2e-59
Identities = 126/282 (44%), Positives = 182/282 (64%), Gaps = 1/282 (0%)
Frame = +1
Query: 220 IVGNLFQLGENNLHIRLAEMAQVHGPLMSLRLGQQILIVGSSATAASEILKTHDHVLSGR 399
I+GNL +G N H+ LAE+A++HGPL+SLRLG Q+L+VGSSA AA+EILKTHD LS R
Sbjct: 53 IIGNLLHVG-NQPHVSLAEIAKIHGPLISLRLGTQLLVVGSSAKAAAEILKTHDRFLSAR 111
Query: 400 PVSQLLQRKVPTAHNRNLVLTSECNDDWRMLRNIYRSELFSRKALESSAKMRENKVMEMV 579
V Q++ R+ LV E D W++LR + R+ELFS KA+ESSA +RE KV E++
Sbjct: 112 HVPQVIPRESHVLRRVALVWCPESIDTWKLLRGLCRTELFSAKAIESSATLREKKVGELM 171
Query: 580 KYLGSKEGEDIIIKNVAFANSVNILGNASLSIDLVDFEGNGIGEGIMDSVKRLNKLAAKP 759
+L ++EG+ + I V F+ N + N S DL E G+ G+ V++L L A P
Sbjct: 172 DFLVAREGKVVSIGEVVFSTVFNTISNLLFSNDLAGLEEKGMSSGLKSHVRKLMLLVATP 231
Query: 760 ILADMYPILSRWDLQGWYKQLMHIIEREFGGVWEDSLKMKRNIVEFLGPK-DFVDILIEK 936
+AD YPI + D QG ++L ++E F +W ++K +RN PK DF+D+ +
Sbjct: 232 NIADFYPIFAGLDPQGLRRKLSKLVEETF-AIWAINIKERRNSYVHDSPKRDFLDVFLAN 290
Query: 937 GFTNQQINPIMEELFAAGTESTSASIEWLMVELLRNPEVMQK 1062
GF + QIN + ELF+AGT++T+ +IEW + E+L+N EVM+K
Sbjct: 291 GFDDDQINWLAAELFSAGTDTTATTIEWAVAEILKNKEVMKK 332
>emb|CAN62647.1| hypothetical protein VITISV_013219 [Vitis vinifera]
Length = 498
Score = 235 bits (599), Expect = 2e-59
Identities = 129/304 (42%), Positives = 187/304 (61%), Gaps = 2/304 (0%)
Frame = +1
Query: 157 MITKHRVSKELXXXXXXXXXXIVGNLFQLGENNLHIRLAEMAQVHGPLMSLRLGQQILIV 336
+I KH K I+GNL Q+G+N H +LA +A++HGPLMSLRLG Q+++V
Sbjct: 26 VILKHIFLKPPPLPPGPYPWPIIGNLLQMGKNP-HAKLANLAKLHGPLMSLRLGTQLMVV 84
Query: 337 GSSATAASEILKTHDHVLSGRPVSQLLQRKVPTAHNRNLVLTSECNDDWRMLRNIYRSEL 516
SS AA E+LKTHD LSGR +S + K P ++ ++V +CN +W+ LR I R EL
Sbjct: 85 ASSPAAAMEVLKTHDRALSGRYLSXSVPVKNPKLNHLSIVFAKDCNTNWKNLRAICRMEL 144
Query: 517 FSRKALESSAKMRENKVMEMVKYLGSKEGEDIIIKNVAFANSVNILGNASLSIDLVDFEG 696
FS KA+ES ++RE KV E+V++L +KEGE + + ++ F NIL N S+DL DFE
Sbjct: 145 FSGKAMESQVELRERKVTELVEFLATKEGEVVKVMDLVFTTICNILSNKFFSMDLCDFED 204
Query: 697 NG-IGEGIMDSVKRLNKLAAKPILADMYPILSRWDLQGWYKQLMHIIEREFGGVWEDSLK 873
G +G + D + + + A P L+D YPIL D+QG ++ + ER WED LK
Sbjct: 205 EGRVGGALKDLIHKNAEFGATPNLSDYYPILGGLDIQGINRKAKEMFER-IPTTWEDILK 263
Query: 874 MKR-NIVEFLGPKDFVDILIEKGFTNQQINPIMEELFAAGTESTSASIEWLMVELLRNPE 1050
+R +DF++ L+E GF + QIN ++ ELF+AG E++S ++EW M EL+RN +
Sbjct: 264 ERRTQRSNRSSHRDFLEALLEIGFEDDQINQVILELFSAGAETSSLTVEWAMAELIRNQD 323
Query: 1051 VMQK 1062
M K
Sbjct: 324 AMDK 327