BLASTX nr result
ID: Atractylodes22_contig00022598
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Atractylodes22_contig00022598
(1360 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAB62808.1| S-adenosyl-methionine-sterol-C- methyltransferase... 644 0.0
gb|ABY49843.1| 24-sterol C-methyltransferase [Gossypium hirsutum] 629 e-178
ref|XP_002510600.1| s-adenosyl-l-methionine:delta24-sterol-C-met... 629 e-178
ref|XP_002307753.1| predicted protein [Populus trichocarpa] gi|1... 628 e-177
ref|NP_173458.1| 24-methylenesterol C-methyltransferase 2 [Arabi... 627 e-177
>gb|AAB62808.1| S-adenosyl-methionine-sterol-C- methyltransferase [Nicotiana tabacum]
Length = 357
Score = 644 bits (1662), Expect = 0.0
Identities = 304/357 (85%), Positives = 333/357 (93%), Gaps = 1/357 (0%)
Frame = -2
Query: 1320 MDSITLVCTVSLLAAG-LYWFVCILGSAEQKGKHAVQLSGGSIQRQNVQDNYNQYWSFFR 1144
MDS+TL+CT +LLAAG LYWFVCILGSAE KGK AVQLSGGSI+++NVQDNY QYWSFFR
Sbjct: 1 MDSLTLICTAALLAAGGLYWFVCILGSAEVKGKRAVQLSGGSIEKENVQDNYKQYWSFFR 60
Query: 1143 RPKEIEKADRVPAFVDTFYNLVTDIYEWGWGQSFHFSPAIPGKSNLESTRIHEEMAVDLI 964
RPKEIE AD+VPAFVDTFYNLVTDIYEWGWGQSFHFSP+IPGKS+ E+TRIHEEMAVDLI
Sbjct: 61 RPKEIETADKVPAFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHREATRIHEEMAVDLI 120
Query: 963 GVKPGQKILDAGCGVGGPMRAIAAHSGCNVVGITINEYQVSRAKAHNKKAGLDKLCDVVC 784
GVKPG +ILDAGCGVGGPMRAIAAHS NVVGITINEYQV RA+ HNKKAGLD LC+VVC
Sbjct: 121 GVKPGARILDAGCGVGGPMRAIAAHSRANVVGITINEYQVKRARMHNKKAGLDSLCEVVC 180
Query: 783 GNFLEMPFEDESFDGAYSIEATCHAPKLEDVYGEIFRVLKPGSMYVSYEWVTTELYRGED 604
GNFL+MPF D SFDGAYSIEATCHAPKLE+VY EI+RV+KPGS+YVSYEWVTTELYR ED
Sbjct: 181 GNFLQMPFPDNSFDGAYSIEATCHAPKLEEVYKEIYRVMKPGSLYVSYEWVTTELYRPED 240
Query: 603 PEHVEIIQGIERGDALPGLRSYSDIAEVAKKVGFEVVKEKDLAKPPSHPWWSRLKMGRIA 424
PEHVEII GIERGDALPGLRSY DIAEVAKKVGFEVVKEKDLA+PPS+PWW+RLKMGRIA
Sbjct: 241 PEHVEIIHGIERGDALPGLRSYKDIAEVAKKVGFEVVKEKDLARPPSNPWWTRLKMGRIA 300
Query: 423 YWRNHILVMVLETLGVAPKGTVDVHEMLFKTADYLTRGGDTGIFTPMHMILCKKPEK 253
YWRNHILV +L LG+APKGTVDVHEMLF TADYL++GG+ GIF+PMHMILC+KPE+
Sbjct: 301 YWRNHILVTILAFLGIAPKGTVDVHEMLFVTADYLSKGGEKGIFSPMHMILCRKPEE 357
>gb|ABY49843.1| 24-sterol C-methyltransferase [Gossypium hirsutum]
Length = 361
Score = 629 bits (1622), Expect = e-178
Identities = 295/361 (81%), Positives = 328/361 (90%), Gaps = 1/361 (0%)
Frame = -2
Query: 1320 MDSITLVCTVSLLAAGLYWFVCILGSAEQKGKHAVQLSGGSIQRQNVQDNYNQYWSFFRR 1141
MDS++L CT +LLA GLYWFVC+LG AEQKGK AV LSGGSI + V+DNY QYWSFFRR
Sbjct: 1 MDSLSLFCTGALLAGGLYWFVCVLGPAEQKGKRAVDLSGGSISAEKVRDNYKQYWSFFRR 60
Query: 1140 PKEIEKADRVPAFVDTFYNLVTDIYEWGWGQSFHFSPAIPGKSNLESTRIHEEMAVDLIG 961
PKEIE A++VP FVDTFYNLVTDIYEWGWGQSFHFSP+IPGKS+ ++TR+HEEMAVDLI
Sbjct: 61 PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEMAVDLID 120
Query: 960 VKPGQKILDAGCGVGGPMRAIAAHSGCNVVGITINEYQVSRAKAHNKKAGLDKLCDVVCG 781
VKPG +ILD GCGVGGPMRAIAAHS VVGITIN+YQV+RA+ HNKKAGLD LC+VVCG
Sbjct: 121 VKPGDRILDVGCGVGGPMRAIAAHSQAKVVGITINDYQVNRARMHNKKAGLDSLCEVVCG 180
Query: 780 NFLEMPFEDESFDGAYSIEATCHAPKLEDVYGEIFRVLKPGSMYVSYEWVTTELYRGEDP 601
NFLEMPF+D SFDGAYSIEATCHAPKLEDVY E+FRVLKPGS+YVSYEWVTTE YR ++P
Sbjct: 181 NFLEMPFQDNSFDGAYSIEATCHAPKLEDVYAEVFRVLKPGSLYVSYEWVTTEKYRADNP 240
Query: 600 EHVEIIQGIERGDALPGLRSYSDIAEVAKKVGFEVVKEKDLAKPPSHPWWSRLKMGRIAY 421
EHVE+IQGIERGDALPGLRSYSDIAE AKKVGFEVVKEKDLAKPPS PWW+RLKMGRIAY
Sbjct: 241 EHVEVIQGIERGDALPGLRSYSDIAEAAKKVGFEVVKEKDLAKPPSLPWWTRLKMGRIAY 300
Query: 420 WRNHILVMVLETLGVAPKGTVDVHEMLFKTADYLTRGGDTGIFTPMHMILCKKP-EKPQE 244
WRNHILV+VL +G+APKGTVDVH+MLFKTADYLTRGGD+GIF+PMHMIL +KP E P +
Sbjct: 301 WRNHILVIVLAAIGIAPKGTVDVHDMLFKTADYLTRGGDSGIFSPMHMILLRKPKEAPSK 360
Query: 243 S 241
S
Sbjct: 361 S 361
>ref|XP_002510600.1| s-adenosyl-l-methionine:delta24-sterol-C-methyltransferase, putative
[Ricinus communis] gi|223551301|gb|EEF52787.1|
s-adenosyl-l-methionine:delta24-sterol-C-
methyltransferase, putative [Ricinus communis]
Length = 361
Score = 629 bits (1621), Expect = e-178
Identities = 296/361 (81%), Positives = 324/361 (89%), Gaps = 1/361 (0%)
Frame = -2
Query: 1320 MDSITLVCTVSLLAAGLYWFVCILGSAEQKGKHAVQLSGGSIQRQNVQDNYNQYWSFFRR 1141
MDS +L CT +LLA GLYWFVCILG AE KGK AV LSGGSI + VQDNY QYWSFFRR
Sbjct: 1 MDSFSLFCTGALLAVGLYWFVCILGPAEVKGKRAVDLSGGSISAEKVQDNYKQYWSFFRR 60
Query: 1140 PKEIEKADRVPAFVDTFYNLVTDIYEWGWGQSFHFSPAIPGKSNLESTRIHEEMAVDLIG 961
PKEIE A++VP FVDTFYNLVTDIYEWGWGQSFHFSP++PGKS+ ++TR+HEEMAVDLI
Sbjct: 61 PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSVPGKSHRDATRLHEEMAVDLID 120
Query: 960 VKPGQKILDAGCGVGGPMRAIAAHSGCNVVGITINEYQVSRAKAHNKKAGLDKLCDVVCG 781
VKPG +ILD GCGVGGPMRAIAAHS NVVGITINEYQV+RA+ HNKKAGLD LC+VVCG
Sbjct: 121 VKPGDRILDVGCGVGGPMRAIAAHSRANVVGITINEYQVNRARLHNKKAGLDSLCEVVCG 180
Query: 780 NFLEMPFEDESFDGAYSIEATCHAPKLEDVYGEIFRVLKPGSMYVSYEWVTTELYRGEDP 601
NFLEMPF D SFDGAYSIEATCHAPKLE+VY EIFRVLKPGS+YVSYEWVTT+ YR D
Sbjct: 181 NFLEMPFPDNSFDGAYSIEATCHAPKLEEVYAEIFRVLKPGSLYVSYEWVTTDKYRASDS 240
Query: 600 EHVEIIQGIERGDALPGLRSYSDIAEVAKKVGFEVVKEKDLAKPPSHPWWSRLKMGRIAY 421
EHVEIIQGIERGDALPGLR+Y DIAE A+KVGFEVVKEKDLAKPP+ PWW+RLKMGRIAY
Sbjct: 241 EHVEIIQGIERGDALPGLRNYIDIAETARKVGFEVVKEKDLAKPPAQPWWTRLKMGRIAY 300
Query: 420 WRNHILVMVLETLGVAPKGTVDVHEMLFKTADYLTRGGDTGIFTPMHMILCKKPE-KPQE 244
WRNHILV +L +G+APKGTVDVHEMLFKTADYLTRGG+TGIFTPMHMILC+KPE KP +
Sbjct: 301 WRNHILVTMLSAIGIAPKGTVDVHEMLFKTADYLTRGGETGIFTPMHMILCRKPEKKPSD 360
Query: 243 S 241
S
Sbjct: 361 S 361
>ref|XP_002307753.1| predicted protein [Populus trichocarpa] gi|118482368|gb|ABK93107.1|
unknown [Populus trichocarpa] gi|222857202|gb|EEE94749.1|
predicted protein [Populus trichocarpa]
Length = 364
Score = 628 bits (1619), Expect = e-177
Identities = 292/359 (81%), Positives = 323/359 (89%)
Frame = -2
Query: 1320 MDSITLVCTVSLLAAGLYWFVCILGSAEQKGKHAVQLSGGSIQRQNVQDNYNQYWSFFRR 1141
MDS+ L CT +L+A G+YWFVCILG AEQKGK AV LSGGSI + VQDNY QYWSFFR
Sbjct: 1 MDSLALFCTGALIAGGIYWFVCILGPAEQKGKRAVDLSGGSISAEKVQDNYKQYWSFFRS 60
Query: 1140 PKEIEKADRVPAFVDTFYNLVTDIYEWGWGQSFHFSPAIPGKSNLESTRIHEEMAVDLIG 961
PKEIE A++VP FVDTFYNLVTDIYEWGWGQSFHFSP+IPGKS+LE+TR+HEEMAVDLI
Sbjct: 61 PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHLEATRLHEEMAVDLIN 120
Query: 960 VKPGQKILDAGCGVGGPMRAIAAHSGCNVVGITINEYQVSRAKAHNKKAGLDKLCDVVCG 781
VKPG +ILD GCGVGGPMRAIAAHS VVGITIN+YQVSRA+ HNKKAGLD LC+VV G
Sbjct: 121 VKPGDRILDVGCGVGGPMRAIAAHSRAKVVGITINDYQVSRARTHNKKAGLDSLCEVVQG 180
Query: 780 NFLEMPFEDESFDGAYSIEATCHAPKLEDVYGEIFRVLKPGSMYVSYEWVTTELYRGEDP 601
NFLEMPF + SFDGAYSIEATCHAPKLE+VY EIFRVLKPGS+YVSYEWVTT+ Y+ DP
Sbjct: 181 NFLEMPFPENSFDGAYSIEATCHAPKLEEVYAEIFRVLKPGSLYVSYEWVTTDKYKSSDP 240
Query: 600 EHVEIIQGIERGDALPGLRSYSDIAEVAKKVGFEVVKEKDLAKPPSHPWWSRLKMGRIAY 421
EHVE+IQGIERGDALPGLR+YSDIAE A+KVGFEVVKEKDLAKPP+ PWW+RL+MGRIAY
Sbjct: 241 EHVEVIQGIERGDALPGLRNYSDIAETARKVGFEVVKEKDLAKPPAQPWWTRLQMGRIAY 300
Query: 420 WRNHILVMVLETLGVAPKGTVDVHEMLFKTADYLTRGGDTGIFTPMHMILCKKPEKPQE 244
WRNHI+V +L LG+APKGTVDVHEMLFKTADYLT+GGDTGIFTPMHMILC+KPEK E
Sbjct: 301 WRNHIVVTILSALGIAPKGTVDVHEMLFKTADYLTKGGDTGIFTPMHMILCRKPEKKTE 359
>ref|NP_173458.1| 24-methylenesterol C-methyltransferase 2 [Arabidopsis thaliana]
gi|62900893|sp|Q39227.2|SMT2_ARATH RecName:
Full=24-methylenesterol C-methyltransferase 2;
Short=24-sterol C-methyltransferase 2;
Short=Sterol-C-methyltransferase 2; AltName: Full=Protein
COTYLEDON VASCULAR PATTERN 1
gi|9558593|gb|AAF88156.1|AC026234_7 Identical to
24-sterol C-methyltransferase from Arabidopsis thaliana
gi|2129517 and is a member of the ubiE/COQ5
methyltransferase family PF|01209. ESTs gb|T42228,
gb|T46520, gb|T41746, gb|N38458, gb|AI993515,
gb|AA389843, gb|AI099890, gb|AI099653 come from this gene
[Arabidopsis thaliana]
gi|12083242|gb|AAG48780.1|AF332417_1 putative
sterol-C-methyltransferase [Arabidopsis thaliana]
gi|15028255|gb|AAK76716.1| putative
sterol-C-methyltransferase [Arabidopsis thaliana]
gi|21281107|gb|AAM45009.1| putative
sterol-C-methyltransferase [Arabidopsis thaliana]
gi|332191841|gb|AEE29962.1| 24-methylenesterol
C-methyltransferase 2 [Arabidopsis thaliana]
Length = 361
Score = 627 bits (1617), Expect = e-177
Identities = 289/360 (80%), Positives = 327/360 (90%)
Frame = -2
Query: 1320 MDSITLVCTVSLLAAGLYWFVCILGSAEQKGKHAVQLSGGSIQRQNVQDNYNQYWSFFRR 1141
MDS+TL T +L+A G+YWF+C+LG AE+KGK AV LSGGSI + VQDNY QYWSFFRR
Sbjct: 1 MDSLTLFFTGALVAVGIYWFLCVLGPAERKGKRAVDLSGGSISAEKVQDNYKQYWSFFRR 60
Query: 1140 PKEIEKADRVPAFVDTFYNLVTDIYEWGWGQSFHFSPAIPGKSNLESTRIHEEMAVDLIG 961
PKEIE A++VP FVDTFYNLVTDIYEWGWGQSFHFSP+IPGKS+ ++TR+HEEMAVDLI
Sbjct: 61 PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHKDATRLHEEMAVDLIQ 120
Query: 960 VKPGQKILDAGCGVGGPMRAIAAHSGCNVVGITINEYQVSRAKAHNKKAGLDKLCDVVCG 781
VKPGQKILD GCGVGGPMRAIA+HS NVVGITINEYQV+RA+ HNKKAGLD LC+VVCG
Sbjct: 121 VKPGQKILDVGCGVGGPMRAIASHSRANVVGITINEYQVNRARLHNKKAGLDALCEVVCG 180
Query: 780 NFLEMPFEDESFDGAYSIEATCHAPKLEDVYGEIFRVLKPGSMYVSYEWVTTELYRGEDP 601
NFL+MPF+D SFDGAYSIEATCHAPKLE+VY EI+RVLKPGSMYVSYEWVTTE ++ ED
Sbjct: 181 NFLQMPFDDNSFDGAYSIEATCHAPKLEEVYAEIYRVLKPGSMYVSYEWVTTEKFKAEDD 240
Query: 600 EHVEIIQGIERGDALPGLRSYSDIAEVAKKVGFEVVKEKDLAKPPSHPWWSRLKMGRIAY 421
EHVE+IQGIERGDALPGLR+Y DIAE AKKVGFE+VKEKDLA PP+ PWW+RLKMGR+AY
Sbjct: 241 EHVEVIQGIERGDALPGLRAYVDIAETAKKVGFEIVKEKDLASPPAEPWWTRLKMGRLAY 300
Query: 420 WRNHILVMVLETLGVAPKGTVDVHEMLFKTADYLTRGGDTGIFTPMHMILCKKPEKPQES 241
WRNHI+V +L +GVAPKGTVDVHEMLFKTADYLTRGG+TGIF+PMHMILC+KPE P+ES
Sbjct: 301 WRNHIVVQILSAVGVAPKGTVDVHEMLFKTADYLTRGGETGIFSPMHMILCRKPESPEES 360