BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes22_contig00020199
         (2072 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AFJ04119.1| mitochondrial protoporphyrinogen IX oxidase, part...   481   e-153
ref|XP_002263321.1| PREDICTED: protoporphyrinogen oxidase, mitoc...   382   e-122
sp|O24164.1|PPOM_TOBAC RecName: Full=Protoporphyrinogen oxidase,...   375   e-121
emb|CAN69962.1| hypothetical protein VITISV_008740 [Vitis vinifera]   373   e-118
pdb|1SEZ|A Chain A, Crystal Structure Of Protoporphyrinogen Ix O...   365   e-118

>gb|AFJ04119.1| mitochondrial protoporphyrinogen IX oxidase, partial [Ambrosia
           artemisiifolia]
          Length = 467

 Score =  481 bits (1239), Expect(2) = e-153
 Identities = 246/293 (83%), Positives = 259/293 (88%)
 Frame = -2

Query: 880 TETEAXXXXXXXXXXXXDKQQFPISQHKRYIVRNGKPVLIPSNPIALIRSSFLSTQSKFQ 701
           TE+EA            DKQQFPISQHKRYIVRNGKPVLIPSNPIALIRSSFLSTQSK Q
Sbjct: 46  TESEADVSSLIDDLGLRDKQQFPISQHKRYIVRNGKPVLIPSNPIALIRSSFLSTQSKVQ 105

Query: 700 VLLEPFLWKKKNSSDTQESVRGFFQRHFGKEVVEYLIDPVVAGTSGGDPESLSMRHAFPE 521
           +LLEPFLWKK  SSD  ESV GFFQRHFGKEVVEYLIDPVVAGTSGGDPESLSMRHAFPE
Sbjct: 106 ILLEPFLWKKTKSSDEPESVGGFFQRHFGKEVVEYLIDPVVAGTSGGDPESLSMRHAFPE 165

Query: 520 LWDLERRFGSVISGAVQSMVSSRGGKRSPSGNSKSRRGSFSFLGGMQILTSALCKEVGQH 341
           LWDLERRFGS+ISGA QSMVSSRGGKR PSGNSK RRGSFSF GGMQ LT AL KE+G H
Sbjct: 166 LWDLERRFGSIISGAFQSMVSSRGGKRKPSGNSKRRRGSFSFFGGMQTLTDALSKEIGPH 225

Query: 340 ELNLQSKVLEMSYSCNDNSEIGNWSICCAPAQNKQLNQQPFDAVIMTAPLGNVKEMKITK 161
           E+NLQSKVLEMSYSC+DN+ +GNWSI CAP QNKQ  QQ FDAVIMTAPL N+KEMKITK
Sbjct: 226 EINLQSKVLEMSYSCDDNA-VGNWSIYCAPDQNKQF-QQSFDAVIMTAPLNNLKEMKITK 283

Query: 160 RGSPFLLNFIPEVSYMPVSVIISTFKKENVKRPLEGFGVLVPAKEQENGLKTL 2
            GSPFLLNFIPEVSY+P+SVIISTFKKENVK+PLEGFGVLVPAKEQENGL+TL
Sbjct: 284 TGSPFLLNFIPEVSYLPISVIISTFKKENVKQPLEGFGVLVPAKEQENGLRTL 336



 Score = 88.6 bits (218), Expect(2) = e-153
 Identities = 40/51 (78%), Positives = 46/51 (90%)
 Frame = -1

Query: 1025 GLAAAYKLKVHGMNVTVFEADRRAGGKLRSISQDGLVWDAGANTMTETD*D 873
            G AAAYKLK+HG+NVTVFEAD R GGKLRS+SQDGL+WD GANTMTE++ D
Sbjct: 1    GCAAAYKLKLHGLNVTVFEADERVGGKLRSVSQDGLIWDEGANTMTESEAD 51


>ref|XP_002263321.1| PREDICTED: protoporphyrinogen oxidase, mitochondrial [Vitis
           vinifera] gi|296090240|emb|CBI40059.3| unnamed protein
           product [Vitis vinifera]
          Length = 508

 Score =  382 bits (980), Expect(2) = e-122
 Identities = 196/302 (64%), Positives = 234/302 (77%), Gaps = 9/302 (2%)
 Frame = -2

Query: 880 TETEAXXXXXXXXXXXXDKQQFPISQHKRYIVRNGKPVLIPSNPIALIRSSFLSTQSKFQ 701
           TE+E             +KQQFPISQ+KRYIVRNG PVL+PSNPIALI+S+ LS +SKFQ
Sbjct: 72  TESEIEVGSLLDNLRLREKQQFPISQNKRYIVRNGMPVLLPSNPIALIKSNILSAKSKFQ 131

Query: 700 VLLEPFLWKKKNSSDT-----QESVRGFFQRHFGKEVVEYLIDPVVAGTSGGDPESLSMR 536
           ++LEPFLWKK + S       +ESV GFFQRHFGKEVV+YLIDP VAGTSGGDPESLSM 
Sbjct: 132 IILEPFLWKKSDLSKVSDDHMKESVGGFFQRHFGKEVVDYLIDPFVAGTSGGDPESLSMH 191

Query: 535 HAFPELWDLERRFGSVISGAVQSMVSS----RGGKRSPSGNSKSRRGSFSFLGGMQILTS 368
           H FPELW+LE+RFGS+I+GAV S +S+    RG  +  S   K +RGSFSF GGMQ LT 
Sbjct: 192 HTFPELWNLEKRFGSIIAGAVLSKLSAKREKRGETKGSSEKKKRQRGSFSFQGGMQTLTD 251

Query: 367 ALCKEVGQHELNLQSKVLEMSYSCNDNSEIGNWSICCAPAQNKQLNQQPFDAVIMTAPLG 188
            LCKE+G+ +L L+SKVL +SYS  + S + NWS+  A    KQ     FDAVIMTAPL 
Sbjct: 252 TLCKELGKDKLRLKSKVLSLSYSHGEKSALENWSVAYASNPGKQSKDLSFDAVIMTAPLC 311

Query: 187 NVKEMKITKRGSPFLLNFIPEVSYMPVSVIISTFKKENVKRPLEGFGVLVPAKEQENGLK 8
           NV+EMKI K+G+PFLL+F+PEVSY+P+SVII+TFKKENVKRPLEGFGVLVP+KEQ+NGLK
Sbjct: 312 NVREMKIMKKGNPFLLDFLPEVSYLPLSVIITTFKKENVKRPLEGFGVLVPSKEQQNGLK 371

Query: 7   TL 2
           TL
Sbjct: 372 TL 373



 Score = 84.3 bits (207), Expect(2) = e-122
 Identities = 37/49 (75%), Positives = 46/49 (93%)
 Frame = -1

Query: 1025 GLAAAYKLKVHGMNVTVFEADRRAGGKLRSISQDGLVWDAGANTMTETD 879
            GLAAAYKLK+HG+N+T++EA+ RAGGKLRS+SQ GLVWD GANTMTE++
Sbjct: 27   GLAAAYKLKLHGVNITLYEAEERAGGKLRSVSQHGLVWDEGANTMTESE 75


>sp|O24164.1|PPOM_TOBAC RecName: Full=Protoporphyrinogen oxidase, mitochondrial; AltName:
           Full=PX-2; AltName: Full=Protoporphyrinogen IX oxidase
           isozyme II; Short=PPO II; Short=PPX II
           gi|2370335|emb|CAA73866.1| protoporphyrinogen oxidase
           [Nicotiana tabacum] gi|3929920|dbj|BAA34712.1|
           mitochondrial protoporphyrinogen oxidase [Nicotiana
           tabacum] gi|4105188|gb|AAD02291.1| protoporphyrinogen
           oxidase PX-2 [Nicotiana tabacum]
          Length = 504

 Score =  375 bits (964), Expect(2) = e-121
 Identities = 190/300 (63%), Positives = 232/300 (77%), Gaps = 7/300 (2%)
 Frame = -2

Query: 880 TETEAXXXXXXXXXXXXDKQQFPISQHKRYIVRNGKPVLIPSNPIALIRSSFLSTQSKFQ 701
           TE+E             +KQQFP+SQ+KRYI RNG PVL+PSNPI LI+S+FLST SK Q
Sbjct: 70  TESEGDVTFLIDSLGLREKQQFPLSQNKRYIARNGTPVLLPSNPIDLIKSNFLSTGSKLQ 129

Query: 700 VLLEPFLWKKKNSS---DTQESVRGFFQRHFGKEVVEYLIDPVVAGTSGGDPESLSMRHA 530
           +LLEP LWK K  S   D+ ESV GFFQRHFGKEVV+YLIDP VAGT GGDP+SLSM H+
Sbjct: 130 MLLEPILWKNKKLSQVSDSHESVSGFFQRHFGKEVVDYLIDPFVAGTCGGDPDSLSMHHS 189

Query: 529 FPELWDLERRFGSVISGAVQSMVSSRGGKRS----PSGNSKSRRGSFSFLGGMQILTSAL 362
           FPELW+LE+RFGSVI GA++S +S +  K+      S N K +RGSFSFLGGMQ LT A+
Sbjct: 190 FPELWNLEKRFGSVILGAIRSKLSPKNEKKQGPPKTSANKKRQRGSFSFLGGMQTLTDAI 249

Query: 361 CKEVGQHELNLQSKVLEMSYSCNDNSEIGNWSICCAPAQNKQLNQQPFDAVIMTAPLGNV 182
           CK++ + EL L S+VLE+S SC ++S I +WSI  A    +Q  ++ FDAVIMTAPL +V
Sbjct: 250 CKDLREDELRLNSRVLELSCSCTEDSAIDSWSIISASPHKRQSEEESFDAVIMTAPLCDV 309

Query: 181 KEMKITKRGSPFLLNFIPEVSYMPVSVIISTFKKENVKRPLEGFGVLVPAKEQENGLKTL 2
           K MKI KRG+PFLLNFIPEV Y+P+SV+I+TFK+ENVK PLEGFGVLVP+KEQ++GLKTL
Sbjct: 310 KSMKIAKRGNPFLLNFIPEVDYVPLSVVITTFKRENVKYPLEGFGVLVPSKEQQHGLKTL 369



 Score = 89.4 bits (220), Expect(2) = e-121
 Identities = 40/51 (78%), Positives = 48/51 (94%)
 Frame = -1

Query: 1025 GLAAAYKLKVHGMNVTVFEADRRAGGKLRSISQDGLVWDAGANTMTETD*D 873
            GLAAAYKLK+HG+NVTVFEA+ +AGGKLRS+SQDGL+WD GANTMTE++ D
Sbjct: 25   GLAAAYKLKIHGLNVTVFEAEGKAGGKLRSVSQDGLIWDEGANTMTESEGD 75


>emb|CAN69962.1| hypothetical protein VITISV_008740 [Vitis vinifera]
          Length = 809

 Score =  373 bits (957), Expect(2) = e-118
 Identities = 189/280 (67%), Positives = 225/280 (80%), Gaps = 9/280 (3%)
 Frame = -2

Query: 814 PISQHKRYIVRNGKPVLIPSNPIALIRSSFLSTQSKFQVLLEPFLWKKKNSSDT-----Q 650
           PISQ+KRYIVRNG PVL+PSNPIALI+S+ LS +SKFQ++LEPFLWKK + S       +
Sbjct: 106 PISQNKRYIVRNGMPVLLPSNPIALIKSNILSAKSKFQIILEPFLWKKSDLSKVSDDHMK 165

Query: 649 ESVRGFFQRHFGKEVVEYLIDPVVAGTSGGDPESLSMRHAFPELWDLERRFGSVISGAVQ 470
           ESV GFFQRHFGKEVV+YLIDP VAGTSGGDPESLSM H FPELW+LE+RFGS+I+GAV 
Sbjct: 166 ESVGGFFQRHFGKEVVDYLIDPFVAGTSGGDPESLSMHHTFPELWNLEKRFGSIIAGAVL 225

Query: 469 SMVSS----RGGKRSPSGNSKSRRGSFSFLGGMQILTSALCKEVGQHELNLQSKVLEMSY 302
           S +S+    RG  +  S   K +RGSFSF GGMQ LT  LCKE+G+ +L L+SKVL +SY
Sbjct: 226 SKLSAKREKRGETKGSSEKKKRQRGSFSFQGGMQTLTDTLCKELGKDKLRLKSKVLSLSY 285

Query: 301 SCNDNSEIGNWSICCAPAQNKQLNQQPFDAVIMTAPLGNVKEMKITKRGSPFLLNFIPEV 122
           S  + S + NWS+  A    KQ     FDAVIMTAPL NV+EMKI K+G+PFLL+F+PEV
Sbjct: 286 SHGEKSALENWSVAYASNPGKQSKDLSFDAVIMTAPLCNVREMKIMKKGNPFLLDFLPEV 345

Query: 121 SYMPVSVIISTFKKENVKRPLEGFGVLVPAKEQENGLKTL 2
           SY+P+SVII+TFKKENVKRPLEGFGVLVP+KEQ+NGLKTL
Sbjct: 346 SYLPLSVIITTFKKENVKRPLEGFGVLVPSKEQQNGLKTL 385



 Score = 82.8 bits (203), Expect(2) = e-118
 Identities = 37/49 (75%), Positives = 45/49 (91%)
 Frame = -1

Query: 1025 GLAAAYKLKVHGMNVTVFEADRRAGGKLRSISQDGLVWDAGANTMTETD 879
            GLAAAYKLK+HG+N T++EA+ RAGGKLRS+SQ GLVWD GANTMTE++
Sbjct: 27   GLAAAYKLKLHGVNXTLYEAEERAGGKLRSVSQHGLVWDEGANTMTESE 75


>pdb|1SEZ|A Chain A, Crystal Structure Of Protoporphyrinogen Ix Oxidase
           gi|48425679|pdb|1SEZ|B Chain B, Crystal Structure Of
           Protoporphyrinogen Ix Oxidase
          Length = 504

 Score =  365 bits (938), Expect(2) = e-118
 Identities = 186/300 (62%), Positives = 227/300 (75%), Gaps = 7/300 (2%)
 Frame = -2

Query: 880 TETEAXXXXXXXXXXXXDKQQFPISQHKRYIVRNGKPVLIPSNPIALIRSSFLSTQSKFQ 701
           TE+E             +KQQFP+SQ+KRYI RNG PVL+PSNPI LI+S+FLST SK Q
Sbjct: 70  TESEGDVTFLIDSLGLREKQQFPLSQNKRYIARNGTPVLLPSNPIDLIKSNFLSTGSKLQ 129

Query: 700 VLLEPFLWKKKNSS---DTQESVRGFFQRHFGKEVVEYLIDPVVAGTSGGDPESLSMRHA 530
            LLEP LWK K  S   D+ ESV GFFQRHFGKEVV+YLIDP VAGT GGDP+SLS  H+
Sbjct: 130 XLLEPILWKNKKLSQVSDSHESVSGFFQRHFGKEVVDYLIDPFVAGTCGGDPDSLSXHHS 189

Query: 529 FPELWDLERRFGSVISGAVQSMVSSRGGKRS----PSGNSKSRRGSFSFLGGMQILTSAL 362
           FPELW+LE+RFGSVI GA++S +S +  K+      S N K +RGSFSFLGG Q LT A+
Sbjct: 190 FPELWNLEKRFGSVILGAIRSKLSPKNEKKQGPPKTSANKKRQRGSFSFLGGXQTLTDAI 249

Query: 361 CKEVGQHELNLQSKVLEMSYSCNDNSEIGNWSICCAPAQNKQLNQQPFDAVIMTAPLGNV 182
           CK++ + EL L S+VLE+S SC ++S I +WSI  A    +Q  ++ FDAVI TAPL +V
Sbjct: 250 CKDLREDELRLNSRVLELSCSCTEDSAIDSWSIISASPHKRQSEEESFDAVIXTAPLCDV 309

Query: 181 KEMKITKRGSPFLLNFIPEVSYMPVSVIISTFKKENVKRPLEGFGVLVPAKEQENGLKTL 2
           K  KI KRG+PFLLNFIPEV Y+P+SV+I+TFK+ENVK PLEGFGVLVP+KEQ++GLKTL
Sbjct: 310 KSXKIAKRGNPFLLNFIPEVDYVPLSVVITTFKRENVKYPLEGFGVLVPSKEQQHGLKTL 369



 Score = 87.0 bits (214), Expect(2) = e-118
 Identities = 39/51 (76%), Positives = 47/51 (92%)
 Frame = -1

Query: 1025 GLAAAYKLKVHGMNVTVFEADRRAGGKLRSISQDGLVWDAGANTMTETD*D 873
            GLAAAYKLK+HG+NVTVFEA+ +AGGKLRS+SQDGL+WD GANT TE++ D
Sbjct: 25   GLAAAYKLKIHGLNVTVFEAEGKAGGKLRSVSQDGLIWDEGANTXTESEGD 75