BLASTX nr result
ID: Atractylodes22_contig00017077
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Atractylodes22_contig00017077
(1372 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CBL42917.1| kaurene synthase [Helianthus annuus] gi|29835728... 708 0.0
dbj|BAB12441.1| ent-kaurene synthase No1 [Lactuca sativa] 700 0.0
gb|AAD34295.1|AF097311_1 kaurene synthase [Stevia rebaudiana] 686 0.0
gb|AAD34294.1|AF097310_1 kaurene synthase [Stevia rebaudiana] 682 0.0
emb|CBL42916.1| kaurene synthase [Helianthus annuus] gi|29835728... 624 e-176
>emb|CBL42917.1| kaurene synthase [Helianthus annuus] gi|298357288|emb|CBM82409.1|
kaurene synthase [Helianthus annuus]
Length = 772
Score = 708 bits (1828), Expect = 0.0
Identities = 350/451 (77%), Positives = 393/451 (87%)
Frame = +2
Query: 2 LLEKFGNAVPTVYPLDLYVRHCMVDTLERLGIARHFRVEIQNVLDETYRCWVQRDEQIFM 181
LL+KFGNAVPT YPLDLY+R MVDTLERLGIARHF VE++NVLDETYRCWV+RDEQIFM
Sbjct: 259 LLDKFGNAVPTAYPLDLYIRLSMVDTLERLGIARHFSVEVKNVLDETYRCWVERDEQIFM 318
Query: 182 DVATCALAFRVLRTNGYEVSSDPLAEITKEGAYMNSLEESFKDVYAALEVYRASQIIHHE 361
DV TCALAFR+LR NGYEVSSDPLAEIT EGA FKD YAA+EVY ASQ+++ E
Sbjct: 319 DVVTCALAFRLLRINGYEVSSDPLAEITNEGA--------FKDEYAAVEVYNASQVLYPE 370
Query: 362 ESAFGEQNLRMSNFLKRKISTASNRHSKFIHKEVENALKFPFNASLERIATKRNIEHYNV 541
E +FGEQ LR + L+R IST SNR SKFIHKEVENALKFPFN LERI T+RNI+ YNV
Sbjct: 371 ELSFGEQILRSGDILRRMISTDSNRLSKFIHKEVENALKFPFNTGLERINTRRNIDRYNV 430
Query: 542 DETRILKTTYRSSNISNEDYLRLAVEDFNACQSIYRGELRVLERWVVEQNLDKLKFARQK 721
D+TRILKTTYRSSNISN+DYLR AVED+N+CQSIYR EL+ LERWVV+ LD+LKFARQK
Sbjct: 431 DDTRILKTTYRSSNISNKDYLRFAVEDYNSCQSIYREELKGLERWVVDNKLDQLKFARQK 490
Query: 722 TAYCYFSAASTLSSPELSDARISWAKSSILTTVIDDFFDVGGSMDELANLIQSVEKWNVN 901
TAYCYFS A+TLSSPELSDARIS AK+ ILTTV+DDFFDVGG++DELANLIQ VEKWNV+
Sbjct: 491 TAYCYFSVAATLSSPELSDARISRAKNGILTTVVDDFFDVGGTIDELANLIQCVEKWNVD 550
Query: 902 VDNDCCSEEVRIIFLALKDAVCWIGDKAFKWQARDITSHVIEIWLDLMKSTLREAIWLRD 1081
VDNDCCSE+VRI+FLALKDA+CWIGD AFKWQ RD+TSHVI+ WLDLM S LREAIW RD
Sbjct: 551 VDNDCCSEQVRIVFLALKDAICWIGDTAFKWQERDVTSHVIQTWLDLMNSMLREAIWTRD 610
Query: 1082 ECVPTIDEYMENGYISFALGPIVLPALYFVGPRLSEEIVQSSEYHNLFKLLSTRGRLLND 1261
VPTI+EYMEN Y+SFALGPIVLPA+YFVGPRLSEEIV+SSEYH L++L+ST GRLLND
Sbjct: 611 AYVPTINEYMENAYVSFALGPIVLPAIYFVGPRLSEEIVESSEYHYLYELMSTLGRLLND 670
Query: 1262 IQGFKREMKAGKLNAVTLYMSHGKSGVVEAE 1354
I FKRE K GKLNAV L +S+G+SG VE E
Sbjct: 671 IHSFKREYKEGKLNAVALQLSNGESGNVEEE 701
>dbj|BAB12441.1| ent-kaurene synthase No1 [Lactuca sativa]
Length = 788
Score = 700 bits (1806), Expect = 0.0
Identities = 349/451 (77%), Positives = 395/451 (87%)
Frame = +2
Query: 2 LLEKFGNAVPTVYPLDLYVRHCMVDTLERLGIARHFRVEIQNVLDETYRCWVQRDEQIFM 181
LL+KFGNAVPTVYP+DLYVR MVDTLERLGI RHF VEIQNVLDETYRCWVQ D QIFM
Sbjct: 270 LLDKFGNAVPTVYPIDLYVRLSMVDTLERLGIKRHFMVEIQNVLDETYRCWVQGDVQIFM 329
Query: 182 DVATCALAFRVLRTNGYEVSSDPLAEITKEGAYMNSLEESFKDVYAALEVYRASQIIHHE 361
DV TCALAFRVLR+NGYEVSSDPLA+ITKEG YMNS E+ FKDVY +LEVY+ASQII+ E
Sbjct: 330 DVVTCALAFRVLRSNGYEVSSDPLAKITKEGDYMNSPEKPFKDVYTSLEVYKASQIIYQE 389
Query: 362 ESAFGEQNLRMSNFLKRKISTASNRHSKFIHKEVENALKFPFNASLERIATKRNIEHYNV 541
E AF EQNL +++L +SN+ S +I KEV++ALKFPFN SLER++T+RNIEHYN+
Sbjct: 390 ELAFREQNL--TSYLP-----SSNKLSNYILKEVDDALKFPFNGSLERMSTRRNIEHYNL 442
Query: 542 DETRILKTTYRSSNISNEDYLRLAVEDFNACQSIYRGELRVLERWVVEQNLDKLKFARQK 721
+ TRILKTTY SSNISN+DYL+LAV+DFN CQSIY EL+ LERWVVE LDKLKFARQK
Sbjct: 443 NHTRILKTTYSSSNISNKDYLKLAVQDFNECQSIYCEELKDLERWVVENRLDKLKFARQK 502
Query: 722 TAYCYFSAASTLSSPELSDARISWAKSSILTTVIDDFFDVGGSMDELANLIQSVEKWNVN 901
TAYCYFSAAS LSSP+LSDARISWAKSSILTTVIDDFFDVGGSMDEL N + +EKWNVN
Sbjct: 503 TAYCYFSAASFLSSPDLSDARISWAKSSILTTVIDDFFDVGGSMDELVNFVHIIEKWNVN 562
Query: 902 VDNDCCSEEVRIIFLALKDAVCWIGDKAFKWQARDITSHVIEIWLDLMKSTLREAIWLRD 1081
V+NDCCSEEV ++FLALKDAVCWIGDKAFK Q R+ITSHVIEIWLDL+KS LREAIW +D
Sbjct: 563 VENDCCSEEVGVLFLALKDAVCWIGDKAFKIQERNITSHVIEIWLDLVKSMLREAIWAKD 622
Query: 1082 ECVPTIDEYMENGYISFALGPIVLPALYFVGPRLSEEIVQSSEYHNLFKLLSTRGRLLND 1261
+PTI+EYMENGY+SFALGPIVLP LYF+G +LSEE+VQSSEYH L++++ST+GRL+ND
Sbjct: 623 GSIPTINEYMENGYVSFALGPIVLPTLYFLGVKLSEEVVQSSEYHKLYEVMSTQGRLMND 682
Query: 1262 IQGFKREMKAGKLNAVTLYMSHGKSGVVEAE 1354
I FKRE KAGKLNAV LYMS GKSG VE E
Sbjct: 683 IHSFKREKKAGKLNAVALYMSDGKSGSVEEE 713
>gb|AAD34295.1|AF097311_1 kaurene synthase [Stevia rebaudiana]
Length = 784
Score = 686 bits (1771), Expect = 0.0
Identities = 339/451 (75%), Positives = 384/451 (85%)
Frame = +2
Query: 2 LLEKFGNAVPTVYPLDLYVRHCMVDTLERLGIARHFRVEIQNVLDETYRCWVQRDEQIFM 181
LL+KFGNAVPTVYP DL++R MVDT+ERLGI+ HFRVEI+NVLDETYRCWV+RDEQIFM
Sbjct: 262 LLDKFGNAVPTVYPHDLFIRLSMVDTIERLGISHHFRVEIKNVLDETYRCWVERDEQIFM 321
Query: 182 DVATCALAFRVLRTNGYEVSSDPLAEITKEGAYMNSLEESFKDVYAALEVYRASQIIHHE 361
DV TCALAFR+LR NGYEVS DPLAEIT E A KD YAALE Y AS I++ E
Sbjct: 322 DVVTCALAFRLLRINGYEVSPDPLAEITNELA--------LKDEYAALETYHASHILYQE 373
Query: 362 ESAFGEQNLRMSNFLKRKISTASNRHSKFIHKEVENALKFPFNASLERIATKRNIEHYNV 541
+ + G+Q L+ ++FLK IST SNR SK IHKEVENALKFP N LERI T+RNI+ YNV
Sbjct: 374 DLSSGKQILKSADFLKEIISTDSNRLSKLIHKEVENALKFPINTGLERINTRRNIQLYNV 433
Query: 542 DETRILKTTYRSSNISNEDYLRLAVEDFNACQSIYRGELRVLERWVVEQNLDKLKFARQK 721
D TRILKTTY SSNISN DYLRLAVEDF CQSIYR EL+ LERWVVE LD+LKFARQK
Sbjct: 434 DNTRILKTTYHSSNISNTDYLRLAVEDFYTCQSIYREELKGLERWVVENKLDQLKFARQK 493
Query: 722 TAYCYFSAASTLSSPELSDARISWAKSSILTTVIDDFFDVGGSMDELANLIQSVEKWNVN 901
TAYCYFS A+TLSSPELSDARISWAK+ ILTTV+DDFFD+GG++DEL NLIQ VEKWNV+
Sbjct: 494 TAYCYFSVAATLSSPELSDARISWAKNGILTTVVDDFFDIGGTIDELTNLIQCVEKWNVD 553
Query: 902 VDNDCCSEEVRIIFLALKDAVCWIGDKAFKWQARDITSHVIEIWLDLMKSTLREAIWLRD 1081
VD DCCSE VRI+FLALKDA+CWIGD+AFKWQARD+TSHVI+ WL+LM S LREAIW RD
Sbjct: 554 VDKDCCSEHVRILFLALKDAICWIGDEAFKWQARDVTSHVIQTWLELMNSMLREAIWTRD 613
Query: 1082 ECVPTIDEYMENGYISFALGPIVLPALYFVGPRLSEEIVQSSEYHNLFKLLSTRGRLLND 1261
VPT++EYMEN Y+SFALGPIV PA+YFVGP+LSEEIV+SSEYHNLFKL+ST+GRLLND
Sbjct: 614 AYVPTLNEYMENAYVSFALGPIVKPAIYFVGPKLSEEIVESSEYHNLFKLMSTQGRLLND 673
Query: 1262 IQGFKREMKAGKLNAVTLYMSHGKSGVVEAE 1354
I FKRE K GKLNAV L++S+G+SG VE E
Sbjct: 674 IHSFKREFKEGKLNAVALHLSNGESGKVEEE 704
>gb|AAD34294.1|AF097310_1 kaurene synthase [Stevia rebaudiana]
Length = 784
Score = 682 bits (1760), Expect = 0.0
Identities = 337/451 (74%), Positives = 385/451 (85%)
Frame = +2
Query: 2 LLEKFGNAVPTVYPLDLYVRHCMVDTLERLGIARHFRVEIQNVLDETYRCWVQRDEQIFM 181
LL+KFGNAVPTVYPLDLY+R MVDT+ERLGI+ HFRVEI+NVLDETYRCWV+RDEQIFM
Sbjct: 262 LLDKFGNAVPTVYPLDLYIRLSMVDTIERLGISHHFRVEIKNVLDETYRCWVERDEQIFM 321
Query: 182 DVATCALAFRVLRTNGYEVSSDPLAEITKEGAYMNSLEESFKDVYAALEVYRASQIIHHE 361
DV TCALAFR+LR +GY+VS D LAEIT E A FKD YAALE Y ASQI++ E
Sbjct: 322 DVVTCALAFRLLRIHGYKVSPDQLAEITNELA--------FKDEYAALETYHASQILYQE 373
Query: 362 ESAFGEQNLRMSNFLKRKISTASNRHSKFIHKEVENALKFPFNASLERIATKRNIEHYNV 541
+ + G+Q L+ ++FLK +ST SNR SK IHKEVENALKFP N LERI T+RNI+ YNV
Sbjct: 374 DLSSGKQILKSADFLKGILSTDSNRLSKLIHKEVENALKFPINTGLERINTRRNIQLYNV 433
Query: 542 DETRILKTTYRSSNISNEDYLRLAVEDFNACQSIYRGELRVLERWVVEQNLDKLKFARQK 721
D TRILKTTY SSNISN YLRLAVEDF CQSIYR EL+ LERWVV+ LD+LKFARQK
Sbjct: 434 DNTRILKTTYHSSNISNTYYLRLAVEDFYTCQSIYREELKGLERWVVQNKLDQLKFARQK 493
Query: 722 TAYCYFSAASTLSSPELSDARISWAKSSILTTVIDDFFDVGGSMDELANLIQSVEKWNVN 901
TAYCYFS A+TLSSPELSDARISWAK+ ILTTV+DDFFD+GG++DEL NLIQ VEKWNV+
Sbjct: 494 TAYCYFSVAATLSSPELSDARISWAKNGILTTVVDDFFDIGGTIDELTNLIQCVEKWNVD 553
Query: 902 VDNDCCSEEVRIIFLALKDAVCWIGDKAFKWQARDITSHVIEIWLDLMKSTLREAIWLRD 1081
VD DCCSE VRI+FLALKDA+CWIGD+AFKWQARD+TSHVI+ WL+LM S LREAIW RD
Sbjct: 554 VDKDCCSEHVRILFLALKDAICWIGDEAFKWQARDVTSHVIQTWLELMNSMLREAIWTRD 613
Query: 1082 ECVPTIDEYMENGYISFALGPIVLPALYFVGPRLSEEIVQSSEYHNLFKLLSTRGRLLND 1261
VPT++EYMEN Y+SFALGPIV PA+YFVGP+LSEEIV+SSEYHNLFKL+ST+GRLLND
Sbjct: 614 AYVPTLNEYMENAYVSFALGPIVKPAIYFVGPKLSEEIVESSEYHNLFKLMSTQGRLLND 673
Query: 1262 IQGFKREMKAGKLNAVTLYMSHGKSGVVEAE 1354
I FKRE K GKLNAV L++S+G+SG VE E
Sbjct: 674 IHSFKREFKEGKLNAVALHLSNGESGKVEEE 704
>emb|CBL42916.1| kaurene synthase [Helianthus annuus] gi|298357286|emb|CBM82408.1|
kaurene synthase [Helianthus annuus]
Length = 774
Score = 624 bits (1608), Expect = e-176
Identities = 309/451 (68%), Positives = 361/451 (80%)
Frame = +2
Query: 2 LLEKFGNAVPTVYPLDLYVRHCMVDTLERLGIARHFRVEIQNVLDETYRCWVQRDEQIFM 181
LLEKFGNAVPTVYPL+LY+R MVDTLERLGIARHF VEI+NVLDETYRCWV+RDEQIFM
Sbjct: 262 LLEKFGNAVPTVYPLELYIRLSMVDTLERLGIARHFSVEIKNVLDETYRCWVERDEQIFM 321
Query: 182 DVATCALAFRVLRTNGYEVSSDPLAEITKEGAYMNSLEESFKDVYAALEVYRASQIIHHE 361
DV TCALAFR+LR NGYEVS DPL EIT EGA FKD YAALEVY ASQI++ +
Sbjct: 322 DVVTCALAFRLLRINGYEVSPDPLEEITNEGA--------FKDEYAALEVYNASQILYPK 373
Query: 362 ESAFGEQNLRMSNFLKRKISTASNRHSKFIHKEVENALKFPFNASLERIATKRNIEHYNV 541
E AFGEQ LR +FL+R ST SNR S FI KE ENALK PFN +ERI KR I+HY+V
Sbjct: 374 ELAFGEQILRSGDFLRRITSTDSNRLSTFIQKEAENALKVPFNTGIERINVKRYIDHYHV 433
Query: 542 DETRILKTTYRSSNISNEDYLRLAVEDFNACQSIYRGELRVLERWVVEQNLDKLKFARQK 721
D TRILKTTYRSSNISNEDYL+ AVEDFN+CQSIY E++ +RW+ E LD+L FAR+K
Sbjct: 434 DHTRILKTTYRSSNISNEDYLKFAVEDFNSCQSIYLEEMKGFKRWLAETKLDQLTFAREK 493
Query: 722 TAYCYFSAASTLSSPELSDARISWAKSSILTTVIDDFFDVGGSMDELANLIQSVEKWNVN 901
+AYCYF+AA+TL +PELSDAR+SWAK+SILT + DDF D G ++DE NL+ VEKWNV+
Sbjct: 494 SAYCYFTAAATLPAPELSDARLSWAKNSILTAITDDFIDGGATIDESINLVYCVEKWNVD 553
Query: 902 VDNDCCSEEVRIIFLALKDAVCWIGDKAFKWQARDITSHVIEIWLDLMKSTLREAIWLRD 1081
VD DCCSE VRI+F A+KDA+CW+GD FKWQ RD+TSHV +IWLDL+ S L EAIW RD
Sbjct: 554 VDKDCCSETVRILFSAIKDAICWMGDAGFKWQERDVTSHVTQIWLDLLNSMLTEAIWRRD 613
Query: 1082 ECVPTIDEYMENGYISFALGPIVLPALYFVGPRLSEEIVQSSEYHNLFKLLSTRGRLLND 1261
PT+DEYM+N +SFALGPIVL LYFVGP+LSEEIV+SSEYHN+F +S GRLLND
Sbjct: 614 AYTPTMDEYMKNASVSFALGPIVLNTLYFVGPKLSEEIVESSEYHNIFDQMSVMGRLLND 673
Query: 1262 IQGFKREMKAGKLNAVTLYMSHGKSGVVEAE 1354
I F RE+K +LNA+ L + +G+S E E
Sbjct: 674 IYSFMREIKE-ELNAIELQLRNGESATGEEE 703