BLASTX nr result
ID: Atractylodes22_contig00015584
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Atractylodes22_contig00015584
(1615 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002285105.1| PREDICTED: isoflavone 2'-hydroxylase-like [V... 591 e-166
ref|XP_002283235.1| PREDICTED: isoflavone 2'-hydroxylase [Vitis ... 565 e-158
ref|XP_002510130.1| cytochrome P450, putative [Ricinus communis]... 555 e-155
ref|XP_002334060.1| cytochrome P450 [Populus trichocarpa] gi|222... 555 e-155
ref|XP_002302426.1| cytochrome P450 [Populus trichocarpa] gi|222... 552 e-154
>ref|XP_002285105.1| PREDICTED: isoflavone 2'-hydroxylase-like [Vitis vinifera]
Length = 499
Score = 591 bits (1523), Expect = e-166
Identities = 292/500 (58%), Positives = 375/500 (75%), Gaps = 1/500 (0%)
Frame = -1
Query: 1588 LLTVIYLLTKYILHNLHHLPPTPFPSLPLIGHLHLLNKPLHRSLAKLSDRYGSVLFLRFG 1409
L V+Y+ T + LH +LPP+PFP+LPLIGHL+LL KPLHR+L+K+SDR+G +LFLRFG
Sbjct: 10 LFFVLYVFTSHFLHKFRNLPPSPFPTLPLIGHLYLLKKPLHRTLSKISDRHGPILFLRFG 69
Query: 1408 CRPVLHVVSPLAAEECFSRNDIVFANRPPLLSGKYFGYNYTSLPWAPYGNHWQNLRRISA 1229
RPVL V SP A+EECF++ND+VFANRP L++GK+ GYNYTS+ WAP+G+HW+NLRRIS+
Sbjct: 70 SRPVLLVSSPSASEECFTKNDVVFANRPRLIAGKHLGYNYTSMSWAPHGDHWRNLRRISS 129
Query: 1228 LEILSSTRLDTLSHIRLQEIRSLTRRLFLAAGKNSDQIFDLRSEFFGFVFNVMTRMMGGK 1049
EILSS RL TLS IR E+RSL R LF KN Q+ +++S FF NVM +M+GGK
Sbjct: 130 FEILSSNRLQTLSGIRSDEVRSLVRWLF----KNQSQMVEMKSAFFEMTLNVMMKMIGGK 185
Query: 1048 TYYGLEIGNLEEAKKFRDLVTDSNQLNAQSGMADMLPILRWFVAGKLEKKYAAVQKRRDE 869
YYG IG +EEA+ FR++V+++ QL + M D LPIL W E++ +QKRR+
Sbjct: 186 RYYGENIGEVEEARMFREMVSETFQLAGATNMVDFLPILGWLGLKGTERRLIKLQKRRES 245
Query: 868 FMQEWIDEFRADGDGLGNGEGK-KTLLQVLLSLQDADPEYYTDKEIKSLSQALLHGGIST 692
F+Q I+E R G N EG+ KT+++VLLSLQ+ +PEYYTD+ I+ L ++L GG T
Sbjct: 246 FIQNLIEEHRRKG---SNCEGRQKTMIEVLLSLQETEPEYYTDEIIRGLMLSMLTGGTDT 302
Query: 691 SVETMEWAMSLLLNNPHVLSKAQIEIDNLVGHDRLVGELDLSKLPYLQYIIKETLRMHPP 512
S TMEWA+SLLLNNP VL KA EID+ +GHDRL+ ELDL++LPYL+ IIKETLRM+P
Sbjct: 303 SAGTMEWALSLLLNNPKVLKKAHQEIDDRLGHDRLIEELDLAQLPYLRSIIKETLRMYPA 362
Query: 511 APLLLPHESSKDCIVGGYRVPQGTMLLVNVWAIQNDPKTWDRPELFKPERFEVKENSVNV 332
PLL+PHESSK+C VGG+R+PQGTMLLVN+WAIQ+D K W P F+PERFE E
Sbjct: 363 GPLLVPHESSKECSVGGFRIPQGTMLLVNLWAIQSDHKIWGDPTEFRPERFEGVEGD--- 419
Query: 331 KEGLTMLPFGHGRRRCPGENLGLRIVELSLASIIQCFDWKRVGEALVDMTESGGFTAPKA 152
++G +PFG GRR CPGE L +RIV L+L S+IQCFDW+RV E +VDMTE GG T PKA
Sbjct: 420 RDGFKFVPFGSGRRGCPGEALAIRIVGLALGSLIQCFDWERVDEQMVDMTEGGGLTLPKA 479
Query: 151 QPLMAKYQTRSIIVDHLSQI 92
QPL+AK + R +V+ SQ+
Sbjct: 480 QPLLAKCRPRPTMVNLFSQL 499
>ref|XP_002283235.1| PREDICTED: isoflavone 2'-hydroxylase [Vitis vinifera]
Length = 498
Score = 565 bits (1455), Expect = e-158
Identities = 283/499 (56%), Positives = 366/499 (73%)
Frame = -1
Query: 1588 LLTVIYLLTKYILHNLHHLPPTPFPSLPLIGHLHLLNKPLHRSLAKLSDRYGSVLFLRFG 1409
+ +Y+ T + LH LH+LPP+PFP LPLIGHL+LL KPLHR+L+K+SDR+G +LFLRFG
Sbjct: 10 IFLALYVFTWHFLHKLHNLPPSPFPILPLIGHLYLLKKPLHRNLSKISDRHGPILFLRFG 69
Query: 1408 CRPVLHVVSPLAAEECFSRNDIVFANRPPLLSGKYFGYNYTSLPWAPYGNHWQNLRRISA 1229
RPVL V S AAEECF++NDI+FANRP L++GK+ GYNYT++ APYG+HW+NLRRIS+
Sbjct: 70 YRPVLIVSSHSAAEECFTKNDIIFANRPRLIAGKHLGYNYTAIGTAPYGDHWRNLRRISS 129
Query: 1228 LEILSSTRLDTLSHIRLQEIRSLTRRLFLAAGKNSDQIFDLRSEFFGFVFNVMTRMMGGK 1049
EILSS RL LS IR E+R+L RL KN +Q+ +++S FF NV+ RM+GGK
Sbjct: 130 FEILSSNRLQMLSGIRSDEVRTLICRLV----KNQNQVVEMKSAFFELTLNVIMRMIGGK 185
Query: 1048 TYYGLEIGNLEEAKKFRDLVTDSNQLNAQSGMADMLPILRWFVAGKLEKKYAAVQKRRDE 869
YYG +G +EEA+KFR++V+ + +L A + M D LPIL W EK+ +QK RD
Sbjct: 186 RYYGENVGEVEEARKFREMVSKAFRL-AGTNMVDFLPILGWLGLKGTEKRLMELQKMRDS 244
Query: 868 FMQEWIDEFRADGDGLGNGEGKKTLLQVLLSLQDADPEYYTDKEIKSLSQALLHGGISTS 689
F+Q I+E R G KT+++VLLSLQ+ +PE YTD+ I L ++L G TS
Sbjct: 245 FIQNLIEEHRIKGSKCERRP--KTMIEVLLSLQETEPENYTDEIIGGLMLSMLTAGTDTS 302
Query: 688 VETMEWAMSLLLNNPHVLSKAQIEIDNLVGHDRLVGELDLSKLPYLQYIIKETLRMHPPA 509
TMEWA+SLLLN+P VL KAQ EID +GHDRL+ E+DL++LPYL+ IIKETLRM+P
Sbjct: 303 AGTMEWALSLLLNSPEVLKKAQQEIDVHLGHDRLIEEVDLAQLPYLRSIIKETLRMYPAG 362
Query: 508 PLLLPHESSKDCIVGGYRVPQGTMLLVNVWAIQNDPKTWDRPELFKPERFEVKENSVNVK 329
PLL+PHESSK+C VGG+R+P GTMLLVNVWAI NDPK W P FKPERFE +E +
Sbjct: 363 PLLIPHESSKECFVGGFRIPPGTMLLVNVWAIHNDPKIWAEPTKFKPERFEGEEGE---R 419
Query: 328 EGLTMLPFGHGRRRCPGENLGLRIVELSLASIIQCFDWKRVGEALVDMTESGGFTAPKAQ 149
+GL LPFG GRR CPGE L +R+V L++ S+IQCFDW+RV + +VDMTE G + PKAQ
Sbjct: 420 DGLRFLPFGSGRRGCPGEGLAIRMVGLAMGSLIQCFDWERVDQQMVDMTEGHGLSIPKAQ 479
Query: 148 PLMAKYQTRSIIVDHLSQI 92
PL+AK + R +V+ LSQ+
Sbjct: 480 PLLAKCRPRPTMVNLLSQL 498
>ref|XP_002510130.1| cytochrome P450, putative [Ricinus communis]
gi|223550831|gb|EEF52317.1| cytochrome P450, putative
[Ricinus communis]
Length = 500
Score = 555 bits (1431), Expect = e-155
Identities = 276/499 (55%), Positives = 359/499 (71%)
Frame = -1
Query: 1588 LLTVIYLLTKYILHNLHHLPPTPFPSLPLIGHLHLLNKPLHRSLAKLSDRYGSVLFLRFG 1409
L V+Y+LT + + + + PP+PFP+LPL+GHLHLL KPLHRSL+ LS+RYG VL L+FG
Sbjct: 8 LFFVLYILTNHFFNKIRNHPPSPFPTLPLLGHLHLLKKPLHRSLSTLSNRYGPVLLLQFG 67
Query: 1408 CRPVLHVVSPLAAEECFSRNDIVFANRPPLLSGKYFGYNYTSLPWAPYGNHWQNLRRISA 1229
R VL V SP AAEEC ++ D VFANRP LL+GK+ GYNYTSL WAPYG+ W+NLR++SA
Sbjct: 68 YRRVLVVSSPSAAEECLTKCDTVFANRPRLLAGKHIGYNYTSLAWAPYGDLWRNLRKVSA 127
Query: 1228 LEILSSTRLDTLSHIRLQEIRSLTRRLFLAAGKNSDQIFDLRSEFFGFVFNVMTRMMGGK 1049
LEILSS RL LS IR E++ L +RLF K+ + D++S FF + NVM RM+ GK
Sbjct: 128 LEILSSHRLQLLSSIRRDEVKLLIQRLF-RNNKDCREKVDMKSAFFELMLNVMMRMIAGK 186
Query: 1048 TYYGLEIGNLEEAKKFRDLVTDSNQLNAQSGMADMLPILRWFVAGKLEKKYAAVQKRRDE 869
YYG + +EEAK+FR++V ++ S M D LP++ V G EK+ + KRRD
Sbjct: 187 RYYGENVEQVEEAKRFREIVRETFLAAGTSNMGDFLPLVA--VVGGQEKRLKELGKRRDG 244
Query: 868 FMQEWIDEFRADGDGLGNGEGKKTLLQVLLSLQDADPEYYTDKEIKSLSQALLHGGISTS 689
F+Q+ IDE R + E KT+++VLLSLQ+++PEYYTD+ IKSL LL G TS
Sbjct: 245 FIQDLIDEHRKRMAACSSEERNKTMIEVLLSLQESEPEYYTDETIKSLMLVLLAAGTDTS 304
Query: 688 VETMEWAMSLLLNNPHVLSKAQIEIDNLVGHDRLVGELDLSKLPYLQYIIKETLRMHPPA 509
TMEWAMSLL+NNP L KAQ EID+++GHDRL+ E D SKLPYL II E +RM+P
Sbjct: 305 AATMEWAMSLLVNNPEALKKAQTEIDSVIGHDRLINESDTSKLPYLNCIINEVMRMYPAG 364
Query: 508 PLLLPHESSKDCIVGGYRVPQGTMLLVNVWAIQNDPKTWDRPELFKPERFEVKENSVNVK 329
PLL+PHESS++C +GGYRVP GTMLLVN+W+IQNDP+ W+ P FKPERFE E V+
Sbjct: 365 PLLVPHESSEECFIGGYRVPAGTMLLVNLWSIQNDPRVWEEPRNFKPERFEGCE---GVR 421
Query: 328 EGLTMLPFGHGRRRCPGENLGLRIVELSLASIIQCFDWKRVGEALVDMTESGGFTAPKAQ 149
+G ++PFG GRR CPGE L LR+V L + +++QCFDW+RVG+ ++DMTE G T PKAQ
Sbjct: 422 DGFRLMPFGSGRRSCPGEGLALRMVGLGIGTLLQCFDWERVGKEMIDMTEGVGLTMPKAQ 481
Query: 148 PLMAKYQTRSIIVDHLSQI 92
PL+ + R +V+ LSQ+
Sbjct: 482 PLVVQCSPRPSMVNLLSQL 500
>ref|XP_002334060.1| cytochrome P450 [Populus trichocarpa] gi|222869629|gb|EEF06760.1|
cytochrome P450 [Populus trichocarpa]
Length = 501
Score = 555 bits (1430), Expect = e-155
Identities = 272/503 (54%), Positives = 370/503 (73%), Gaps = 4/503 (0%)
Frame = -1
Query: 1588 LLTVIYLLTKYILHNLHHLPPTPFPSLPLIGHLHLLNKPLHRSLAKLSDRYGSVLFLRFG 1409
L V+Y+LT + + + +LPP+PFP+LP+IGHLHLL KPLHRSL+K+S+R+G V+ L+ G
Sbjct: 8 LYLVLYVLTNHFRNKIQNLPPSPFPALPIIGHLHLLKKPLHRSLSKISNRHGPVVLLQLG 67
Query: 1408 CRPVLHVVSPLAAEECFSRNDIVFANRPPLLSGKYFGYNYTSLPWAPYGNHWQNLRRISA 1229
R VL V SP AAEECF++NDIVFANRP LL+GK+ G NYT+LPWAP+G+ W+NLR+IS+
Sbjct: 68 SRRVLVVSSPSAAEECFTKNDIVFANRPHLLAGKHLGRNYTTLPWAPHGDLWRNLRKISS 127
Query: 1228 LEILSSTRLDTLSHIRLQEIRSLTRRLFLAAGKNSDQIFDLRSEFFGFVFNVMTRMMGGK 1049
LEILSS RL LS IR +E++ L RRLF KN+DQI DL+S FF + NVM RM+ GK
Sbjct: 128 LEILSSNRLQLLSSIRTEEVKLLIRRLF----KNNDQIIDLKSSFFELMLNVMMRMIAGK 183
Query: 1048 TYYGLEIGNLEEAKKFRDLVTDSNQLNAQSGMADMLPILRWFVAGKLEKKYAAVQKRRDE 869
YYG +EE ++FR++VT++ Q++ S + D L +L V G EK++ +Q++RD
Sbjct: 184 RYYGENEAEVEEGRRFREIVTETFQVSGASAVGDFLHVLA--VIGGTEKRFMKLQEKRDG 241
Query: 868 FMQEWIDEFRA----DGDGLGNGEGKKTLLQVLLSLQDADPEYYTDKEIKSLSQALLHGG 701
FMQE +DE R + N KT+++VLL+LQ+++PEYY D+ IK L LL G
Sbjct: 242 FMQELVDEPRRRMGNNKSCFSNERNYKTMIEVLLTLQESEPEYYKDETIKDLMVVLLSAG 301
Query: 700 ISTSVETMEWAMSLLLNNPHVLSKAQIEIDNLVGHDRLVGELDLSKLPYLQYIIKETLRM 521
T+ T+EWA+SLLLNNP +L KAQ EID +VG DRL+ E D++KLPYL +IKET+RM
Sbjct: 302 TDTTAGTVEWALSLLLNNPLILKKAQNEIDKVVGQDRLIDESDVAKLPYLHCVIKETMRM 361
Query: 520 HPPAPLLLPHESSKDCIVGGYRVPQGTMLLVNVWAIQNDPKTWDRPELFKPERFEVKENS 341
+P PLL+PHESS++C+VGG+++P+GTMLLVN+WAIQNDPK WD FKPERF + S
Sbjct: 362 YPVGPLLVPHESSEECVVGGFQIPRGTMLLVNIWAIQNDPKIWDDAAKFKPERF---DGS 418
Query: 340 VNVKEGLTMLPFGHGRRRCPGENLGLRIVELSLASIIQCFDWKRVGEALVDMTESGGFTA 161
V++G ++PFG GRR CPGE L +R+ L+L S++QCF+W RV + +VD+TE G +
Sbjct: 419 EGVRDGFKLMPFGSGRRSCPGEGLAMRMAGLTLGSLLQCFEWDRVSQEMVDLTEGTGLSM 478
Query: 160 PKAQPLMAKYQTRSIIVDHLSQI 92
PKAQPL+A+ +R + + LSQI
Sbjct: 479 PKAQPLLARCTSRPSMANLLSQI 501
>ref|XP_002302426.1| cytochrome P450 [Populus trichocarpa] gi|222844152|gb|EEE81699.1|
cytochrome P450 [Populus trichocarpa]
Length = 501
Score = 552 bits (1422), Expect = e-154
Identities = 274/503 (54%), Positives = 366/503 (72%), Gaps = 4/503 (0%)
Frame = -1
Query: 1588 LLTVIYLLTKYILHNLHHLPPTPFPSLPLIGHLHLLNKPLHRSLAKLSDRYGSVLFLRFG 1409
L V+Y+LT + + + +LPP+PFP+LP+IGHLHLL KPLHRSL+K+S+R+G V+ L+ G
Sbjct: 8 LYLVLYVLTNHFRNKIQNLPPSPFPALPIIGHLHLLKKPLHRSLSKISNRHGPVVLLQLG 67
Query: 1408 CRPVLHVVSPLAAEECFSRNDIVFANRPPLLSGKYFGYNYTSLPWAPYGNHWQNLRRISA 1229
R VL V SP AAEECF++NDIVFANRP LL+GK+ G NYT+L WAP+G+ W+NLR+IS+
Sbjct: 68 SRRVLVVSSPSAAEECFTKNDIVFANRPHLLAGKHLGRNYTTLSWAPHGDLWRNLRKISS 127
Query: 1228 LEILSSTRLDTLSHIRLQEIRSLTRRLFLAAGKNSDQIFDLRSEFFGFVFNVMTRMMGGK 1049
LEILSS RL S IR +E++ L RRLF KN+D+I DL+S FF + NVM RM+ GK
Sbjct: 128 LEILSSNRLQLFSSIRTEEVKFLIRRLF----KNNDEIIDLKSSFFELMLNVMMRMIAGK 183
Query: 1048 TYYGLEIGNLEEAKKFRDLVTDSNQLNAQSGMADMLPILRWFVAGKLEKKYAAVQKRRDE 869
YYG +EE ++FR++VT++ Q++ S + D L +L V G EK+ +Q++RD
Sbjct: 184 RYYGENEAEVEEGRRFREIVTETFQVSGASAVGDFLHVLA--VIGGTEKRLMKLQEKRDG 241
Query: 868 FMQEWIDEFRA----DGDGLGNGEGKKTLLQVLLSLQDADPEYYTDKEIKSLSQALLHGG 701
F+QE +DE R + N KT+++VLL+LQ+++PEYY D+ IK L LL G
Sbjct: 242 FLQELVDEHRRRMGNNKSCFSNERNYKTMIEVLLTLQESEPEYYKDETIKDLMVVLLSAG 301
Query: 700 ISTSVETMEWAMSLLLNNPHVLSKAQIEIDNLVGHDRLVGELDLSKLPYLQYIIKETLRM 521
T+ TMEWA+SLLLNNP +L KAQ EID +VGHDRL+ E D+ KLPYL +IKET+RM
Sbjct: 302 TETTAGTMEWALSLLLNNPLILRKAQNEIDKVVGHDRLIDESDVVKLPYLHCVIKETMRM 361
Query: 520 HPPAPLLLPHESSKDCIVGGYRVPQGTMLLVNVWAIQNDPKTWDRPELFKPERFEVKENS 341
+P PLL+PH SS++C VGG+++P GTMLLVN+WAIQNDPK WD FKPERF E S
Sbjct: 362 YPIGPLLVPHRSSEECGVGGFQIPSGTMLLVNMWAIQNDPKIWDDAAKFKPERF---EGS 418
Query: 340 VNVKEGLTMLPFGHGRRRCPGENLGLRIVELSLASIIQCFDWKRVGEALVDMTESGGFTA 161
V V++G ++PFG GRRRCPGE L +R+V L+L S++QCF+W RV + +VDMT G T
Sbjct: 419 VGVRDGFKLMPFGSGRRRCPGEGLAIRMVGLTLGSLLQCFEWDRVSQEMVDMTGGTGLTM 478
Query: 160 PKAQPLMAKYQTRSIIVDHLSQI 92
PKAQPL+A+ +R + + LSQI
Sbjct: 479 PKAQPLLARCTSRPSMANLLSQI 501