BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes22_contig00013299
         (1711 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q04468.1|TCMO_HELTU RecName: Full=Trans-cinnamate 4-monooxyge...   882   0.0  
gb|ADO16181.1| cytochrome P450 mono-oxygenase [Artemisia annua]       880   0.0  
gb|ACF74449.1| trans-cinnamate 4-monooxygenase [Echinacea angust...   876   0.0  
gb|ACJ37399.1| trans-cinnamate 4-monooxygenase [Echinacea angust...   862   0.0  
dbj|BAJ17666.1| cinnamate 4-hydroxylase [Gynura bicolor]              860   0.0  

>sp|Q04468.1|TCMO_HELTU RecName: Full=Trans-cinnamate 4-monooxygenase; AltName: Full=Cinnamic
            acid 4-hydroxylase; Short=C4H; Short=CA4H; AltName:
            Full=Cytochrome P450 73; AltName: Full=Cytochrome P450C4H
            gi|18859|emb|CAA78982.1| trans-cinnamate 4-monooxygenase
            [Helianthus tuberosus]
          Length = 505

 Score =  882 bits (2278), Expect = 0.0
 Identities = 438/505 (86%), Positives = 449/505 (88%)
 Frame = +3

Query: 48   MDLLLLEKTXXXXXXXXXXXXXXSKLRGKRFKLPPGPIPVPIFGNWLQVGDDLNHRNLTD 227
            MDLLL+EKT              SKLRGK+FKLPPGPIPVPIFGNWLQVGDDLNHRNLTD
Sbjct: 1    MDLLLIEKTLVALFAAIIGAILISKLRGKKFKLPPGPIPVPIFGNWLQVGDDLNHRNLTD 60

Query: 228  LAKKFGEIFLLRMGQRNLVVVSSPDLAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 407
            LAK+FGEI LLRMGQRNLVVVSSP+LAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT
Sbjct: 61   LAKRFGEILLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 408  VYGEHWRKMRRIMTVPFFTNKVVQQYRHGWXXXXXXXXXXXKKNPASATEGIVIRRRLQL 587
            VYGEHWRKMRRIMTVPFFTNKVVQQYR+GW           KKNPA+ATEGIVIRRRLQL
Sbjct: 121  VYGEHWRKMRRIMTVPFFTNKVVQQYRYGWEAEAAAVVDDVKKNPAAATEGIVIRRRLQL 180

Query: 588  MMYNNMFRIMFDRRFESEDDPLFLKLKALNGERSRLAQSFEYNYGDFIPILRPFLRGYLK 767
            MMYNNMFRIMFDRRFESEDDPLFLKLKALNGERSRLAQSFEYNYGDFIPILRPFLR YLK
Sbjct: 181  MMYNNMFRIMFDRRFESEDDPLFLKLKALNGERSRLAQSFEYNYGDFIPILRPFLRNYLK 240

Query: 768  LCKEVKEKRFQLFKDYFVDERKKLGSTKKMDNNQLKCAIDHILEAKEKGEINEDNVLYIV 947
            LCKEVK+KR QLFKDYFVDERKK+GSTKKMDNNQLKCAIDHILEAKEKGEINEDNVLYIV
Sbjct: 241  LCKEVKDKRIQLFKDYFVDERKKIGSTKKMDNNQLKCAIDHILEAKEKGEINEDNVLYIV 300

Query: 948  ENINVAAIETTLWSIEWGIAELVNHPEIQAKLRHELDTKLGPGVQITEPDVQNLPYLQAV 1127
            ENINVAAIETTLWSIEWGIAELVNHPEIQAKLRHELDTKLGPGVQITEPDVQNLPYLQAV
Sbjct: 301  ENINVAAIETTLWSIEWGIAELVNHPEIQAKLRHELDTKLGPGVQITEPDVQNLPYLQAV 360

Query: 1128 IKETLRLRMPIPLLVPHMNLHDAKLGGFDIPAESKILVNAWWLANNPEQWKKXXXXXXXX 1307
            +KETLRLRM IPLLVPHMNLHDAKLGGFDIPAESKILVNAWWLANNP+QWKK        
Sbjct: 361  VKETLRLRMAIPLLVPHMNLHDAKLGGFDIPAESKILVNAWWLANNPDQWKKPEEFRPER 420

Query: 1308 XXXXDAKVEANGNDFRYLPFGVGRRSCPXXXXXXXXXXXXXXRLVQNFELLPPPGQSKID 1487
                +AKVEANGNDFRYLPFGVGRRSCP              RLVQNFELLPPPGQSKID
Sbjct: 421  FLEEEAKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKID 480

Query: 1488 TSEKGGQFSLHILKHSTIVAKPRSF 1562
            T EKGGQFSLHILKHSTIVAKPRSF
Sbjct: 481  TDEKGGQFSLHILKHSTIVAKPRSF 505


>gb|ADO16181.1| cytochrome P450 mono-oxygenase [Artemisia annua]
          Length = 505

 Score =  880 bits (2274), Expect = 0.0
 Identities = 437/505 (86%), Positives = 448/505 (88%)
 Frame = +3

Query: 48   MDLLLLEKTXXXXXXXXXXXXXXSKLRGKRFKLPPGPIPVPIFGNWLQVGDDLNHRNLTD 227
            MDLLLLEKT              SKLRGKRFKLPPGPIPVPIFGNWLQVGDDLNHRNLTD
Sbjct: 1    MDLLLLEKTLLGLLVAILGAIFISKLRGKRFKLPPGPIPVPIFGNWLQVGDDLNHRNLTD 60

Query: 228  LAKKFGEIFLLRMGQRNLVVVSSPDLAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 407
            LAKKFGEIFLLRMGQRNLVVVSSPDLAK+VLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT
Sbjct: 61   LAKKFGEIFLLRMGQRNLVVVSSPDLAKDVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 408  VYGEHWRKMRRIMTVPFFTNKVVQQYRHGWXXXXXXXXXXXKKNPASATEGIVIRRRLQL 587
            VYGEHWRKMRRIMTVPFFTNKVVQQYR GW           KKNPASATEG V+RRRLQL
Sbjct: 121  VYGEHWRKMRRIMTVPFFTNKVVQQYRFGWEAEAAAVVEDVKKNPASATEGTVLRRRLQL 180

Query: 588  MMYNNMFRIMFDRRFESEDDPLFLKLKALNGERSRLAQSFEYNYGDFIPILRPFLRGYLK 767
            MMYNNMFRIMFDRRFESEDDPLFLKLKALNGERSRLAQSFEYNYGDFIPILRPFLRGYLK
Sbjct: 181  MMYNNMFRIMFDRRFESEDDPLFLKLKALNGERSRLAQSFEYNYGDFIPILRPFLRGYLK 240

Query: 768  LCKEVKEKRFQLFKDYFVDERKKLGSTKKMDNNQLKCAIDHILEAKEKGEINEDNVLYIV 947
            LCKEVK+KR QLFKDYFVDERKKLGSTK +DNNQ+KCAIDHILEAK+KGEINEDNVLYIV
Sbjct: 241  LCKEVKDKRLQLFKDYFVDERKKLGSTKSLDNNQIKCAIDHILEAKDKGEINEDNVLYIV 300

Query: 948  ENINVAAIETTLWSIEWGIAELVNHPEIQAKLRHELDTKLGPGVQITEPDVQNLPYLQAV 1127
            ENINVAAIETTLWSIEWGIAELVNHPEIQAKLRHELDTKLGPGVQ+TEPD+QNLPYLQAV
Sbjct: 301  ENINVAAIETTLWSIEWGIAELVNHPEIQAKLRHELDTKLGPGVQVTEPDIQNLPYLQAV 360

Query: 1128 IKETLRLRMPIPLLVPHMNLHDAKLGGFDIPAESKILVNAWWLANNPEQWKKXXXXXXXX 1307
            IKETLRLRM IPLLVPHMNLHDAKLGGFDIPAESKILVNAWWLANNP+QWKK        
Sbjct: 361  IKETLRLRMAIPLLVPHMNLHDAKLGGFDIPAESKILVNAWWLANNPDQWKKPEEFRPER 420

Query: 1308 XXXXDAKVEANGNDFRYLPFGVGRRSCPXXXXXXXXXXXXXXRLVQNFELLPPPGQSKID 1487
                ++KVEANGNDFRYLPFGVGRRSCP              RLVQNFELLPPPG SKID
Sbjct: 421  FLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGVSKID 480

Query: 1488 TSEKGGQFSLHILKHSTIVAKPRSF 1562
            TSEKGGQFSLHILKHSTIVAKPRSF
Sbjct: 481  TSEKGGQFSLHILKHSTIVAKPRSF 505


>gb|ACF74449.1| trans-cinnamate 4-monooxygenase [Echinacea angustifolia]
          Length = 505

 Score =  876 bits (2264), Expect = 0.0
 Identities = 434/505 (85%), Positives = 450/505 (89%)
 Frame = +3

Query: 48   MDLLLLEKTXXXXXXXXXXXXXXSKLRGKRFKLPPGPIPVPIFGNWLQVGDDLNHRNLTD 227
            MDL L+EKT              SKLRGK+FKLPPGPIPVPIFGNWLQVGDDLNHRNLTD
Sbjct: 1    MDLPLIEKTLIALFAAIIGAIVVSKLRGKKFKLPPGPIPVPIFGNWLQVGDDLNHRNLTD 60

Query: 228  LAKKFGEIFLLRMGQRNLVVVSSPDLAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 407
            LAKKFG+IFLLRMGQRNLVVVSSP+LAK+VLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT
Sbjct: 61   LAKKFGQIFLLRMGQRNLVVVSSPELAKDVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 408  VYGEHWRKMRRIMTVPFFTNKVVQQYRHGWXXXXXXXXXXXKKNPASATEGIVIRRRLQL 587
            VYGEHWRKMRRIMTVPFFTNKVVQQYR+GW           KKNPA+ATEG+VIRRRLQL
Sbjct: 121  VYGEHWRKMRRIMTVPFFTNKVVQQYRYGWEAEAAAVVEDVKKNPAAATEGVVIRRRLQL 180

Query: 588  MMYNNMFRIMFDRRFESEDDPLFLKLKALNGERSRLAQSFEYNYGDFIPILRPFLRGYLK 767
            MMYNNMFRIMFDRRFESE+DPLFLKLKALNGERSRLAQSFEYNYGDFIPILRPFLR YLK
Sbjct: 181  MMYNNMFRIMFDRRFESEEDPLFLKLKALNGERSRLAQSFEYNYGDFIPILRPFLRNYLK 240

Query: 768  LCKEVKEKRFQLFKDYFVDERKKLGSTKKMDNNQLKCAIDHILEAKEKGEINEDNVLYIV 947
            LCKEVKEKR QLFKDYFVDERKKLGSTKKMD+NQLKCAIDHILEAK+KGEINEDNVLYIV
Sbjct: 241  LCKEVKEKRIQLFKDYFVDERKKLGSTKKMDDNQLKCAIDHILEAKDKGEINEDNVLYIV 300

Query: 948  ENINVAAIETTLWSIEWGIAELVNHPEIQAKLRHELDTKLGPGVQITEPDVQNLPYLQAV 1127
            ENINVAAIETTLWSIEWGIAELVNHPEIQAKLRHELDTKLGPGVQITEPDVQNLPYLQAV
Sbjct: 301  ENINVAAIETTLWSIEWGIAELVNHPEIQAKLRHELDTKLGPGVQITEPDVQNLPYLQAV 360

Query: 1128 IKETLRLRMPIPLLVPHMNLHDAKLGGFDIPAESKILVNAWWLANNPEQWKKXXXXXXXX 1307
            IKETLRLRM IPLLVPHMNLHDAKLGGFDIPAESKILVNAWWLANNP+QWKK        
Sbjct: 361  IKETLRLRMAIPLLVPHMNLHDAKLGGFDIPAESKILVNAWWLANNPDQWKKPEEFRPER 420

Query: 1308 XXXXDAKVEANGNDFRYLPFGVGRRSCPXXXXXXXXXXXXXXRLVQNFELLPPPGQSKID 1487
                +AKV+ANGNDFRYLPFGVGRRSCP              RLVQNFELLPPPGQSKID
Sbjct: 421  FLEEEAKVDANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKID 480

Query: 1488 TSEKGGQFSLHILKHSTIVAKPRSF 1562
            T+EKGGQFSLHILKHST+VAKPRSF
Sbjct: 481  TAEKGGQFSLHILKHSTVVAKPRSF 505


>gb|ACJ37399.1| trans-cinnamate 4-monooxygenase [Echinacea angustifolia]
          Length = 505

 Score =  862 bits (2228), Expect = 0.0
 Identities = 426/503 (84%), Positives = 443/503 (88%)
 Frame = +3

Query: 48   MDLLLLEKTXXXXXXXXXXXXXXSKLRGKRFKLPPGPIPVPIFGNWLQVGDDLNHRNLTD 227
            MDLLLLEKT              SKLRGKRFKLPPGP+PVPIFGNWLQVGDDLNHRNLTD
Sbjct: 1    MDLLLLEKTLIALFAAIIASIFISKLRGKRFKLPPGPLPVPIFGNWLQVGDDLNHRNLTD 60

Query: 228  LAKKFGEIFLLRMGQRNLVVVSSPDLAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 407
            LAKKFGEI LLRMGQRNLVVVSSP+LAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT
Sbjct: 61   LAKKFGEILLLRMGQRNLVVVSSPNLAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 408  VYGEHWRKMRRIMTVPFFTNKVVQQYRHGWXXXXXXXXXXXKKNPASATEGIVIRRRLQL 587
            VYGEHWRKMRRIMTVPFFTNKVVQQY  GW           +KNP +ATEG+VIRRRLQL
Sbjct: 121  VYGEHWRKMRRIMTVPFFTNKVVQQYHSGWEAEAAAVVEDVRKNPKAATEGVVIRRRLQL 180

Query: 588  MMYNNMFRIMFDRRFESEDDPLFLKLKALNGERSRLAQSFEYNYGDFIPILRPFLRGYLK 767
            MMYNNMFRIMFDRRFESEDDPLFLKLKALNGERSRLAQSFEYNYGDFIPILRPFL+GYLK
Sbjct: 181  MMYNNMFRIMFDRRFESEDDPLFLKLKALNGERSRLAQSFEYNYGDFIPILRPFLKGYLK 240

Query: 768  LCKEVKEKRFQLFKDYFVDERKKLGSTKKMDNNQLKCAIDHILEAKEKGEINEDNVLYIV 947
            LCKEVKEKRFQLFKDYFVDERKKLGSTK MDNNQLKCAIDHIL+AK+KGEINEDNVLYIV
Sbjct: 241  LCKEVKEKRFQLFKDYFVDERKKLGSTKSMDNNQLKCAIDHILDAKDKGEINEDNVLYIV 300

Query: 948  ENINVAAIETTLWSIEWGIAELVNHPEIQAKLRHELDTKLGPGVQITEPDVQNLPYLQAV 1127
            ENINVAAIETTLWSIEWGIAELVNHPEIQAKLRHELDTKLGPGVQ+TEPD+  LPYLQAV
Sbjct: 301  ENINVAAIETTLWSIEWGIAELVNHPEIQAKLRHELDTKLGPGVQVTEPDLDKLPYLQAV 360

Query: 1128 IKETLRLRMPIPLLVPHMNLHDAKLGGFDIPAESKILVNAWWLANNPEQWKKXXXXXXXX 1307
            IKETLRLRM IPLLVPHMNLHDAKLGG+DIPAESKILVNAWWLANNP+QWK+        
Sbjct: 361  IKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPDQWKEPEVFRPER 420

Query: 1308 XXXXDAKVEANGNDFRYLPFGVGRRSCPXXXXXXXXXXXXXXRLVQNFELLPPPGQSKID 1487
                ++KVEANGNDFRYLPFGVGRRSCP              RLVQNFELLPPPGQSK+ 
Sbjct: 421  FLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKVG 480

Query: 1488 TSEKGGQFSLHILKHSTIVAKPR 1556
            T+EKGGQFSLHILKHSTIVAKPR
Sbjct: 481  TTEKGGQFSLHILKHSTIVAKPR 503


>dbj|BAJ17666.1| cinnamate 4-hydroxylase [Gynura bicolor]
          Length = 505

 Score =  860 bits (2221), Expect = 0.0
 Identities = 424/505 (83%), Positives = 442/505 (87%)
 Frame = +3

Query: 48   MDLLLLEKTXXXXXXXXXXXXXXSKLRGKRFKLPPGPIPVPIFGNWLQVGDDLNHRNLTD 227
            MDLLLLEKT              SKLRGK+FKLPPGPIPVPIFGNWLQVGDDLNHRNLTD
Sbjct: 1    MDLLLLEKTLIALFTAIIIAIVISKLRGKKFKLPPGPIPVPIFGNWLQVGDDLNHRNLTD 60

Query: 228  LAKKFGEIFLLRMGQRNLVVVSSPDLAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 407
            +AKKFG+IF LRMGQRNL+VVSSP+LAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT
Sbjct: 61   MAKKFGDIFHLRMGQRNLIVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 408  VYGEHWRKMRRIMTVPFFTNKVVQQYRHGWXXXXXXXXXXXKKNPASATEGIVIRRRLQL 587
            VYGEHWRKMRRIMTVPFFTNKVVQQYR+GW           KKNPA+ATEG+VIRRRLQL
Sbjct: 121  VYGEHWRKMRRIMTVPFFTNKVVQQYRYGWEEEAAAVVEDVKKNPAAATEGVVIRRRLQL 180

Query: 588  MMYNNMFRIMFDRRFESEDDPLFLKLKALNGERSRLAQSFEYNYGDFIPILRPFLRGYLK 767
            MMYNNMFRIMFDRRFESEDDPLFLKLKALNGERSRLAQSFEYNYGDFIPILRPFLR YL 
Sbjct: 181  MMYNNMFRIMFDRRFESEDDPLFLKLKALNGERSRLAQSFEYNYGDFIPILRPFLRKYLN 240

Query: 768  LCKEVKEKRFQLFKDYFVDERKKLGSTKKMDNNQLKCAIDHILEAKEKGEINEDNVLYIV 947
            LCKEVK+KR QLFKDYFVDERKK+GSTKKMD N+LKCAIDHILEAKEKGEINEDNVLYIV
Sbjct: 241  LCKEVKDKRIQLFKDYFVDERKKIGSTKKMDKNELKCAIDHILEAKEKGEINEDNVLYIV 300

Query: 948  ENINVAAIETTLWSIEWGIAELVNHPEIQAKLRHELDTKLGPGVQITEPDVQNLPYLQAV 1127
            ENINVAAIETTLWSIEWG+AELVNHP+IQAKLRHELDTKLGP VQITEPDVQNLPYLQAV
Sbjct: 301  ENINVAAIETTLWSIEWGLAELVNHPKIQAKLRHELDTKLGPSVQITEPDVQNLPYLQAV 360

Query: 1128 IKETLRLRMPIPLLVPHMNLHDAKLGGFDIPAESKILVNAWWLANNPEQWKKXXXXXXXX 1307
            +KETLRLRM IPLLVPHMNLHDAKLGG+DIPAESKILVNAWWLANNP+ WK         
Sbjct: 361  VKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPDHWKNANEFRPER 420

Query: 1308 XXXXDAKVEANGNDFRYLPFGVGRRSCPXXXXXXXXXXXXXXRLVQNFELLPPPGQSKID 1487
                +AKVEANGNDFRYLPFGVGRRSCP              RLVQNFELLPPPGQ KID
Sbjct: 421  FLEEEAKVEANGNDFRYLPFGVGRRSCPGIILALPILAITLGRLVQNFELLPPPGQDKID 480

Query: 1488 TSEKGGQFSLHILKHSTIVAKPRSF 1562
             SEKGGQFSLHILKH T+VAKPRSF
Sbjct: 481  MSEKGGQFSLHILKHYTVVAKPRSF 505