BLASTX nr result
ID: Atractylodes22_contig00012901
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Atractylodes22_contig00012901
(1248 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002300266.1| predicted protein [Populus trichocarpa] gi|2... 429 e-154
ref|XP_002265287.1| PREDICTED: ribosomal RNA large subunit methy... 432 e-150
ref|XP_004136937.1| PREDICTED: dual-specificity RNA methyltransf... 424 e-146
ref|NP_188597.1| radical SAM domain-containing protein [Arabidop... 404 e-141
ref|XP_003558557.1| PREDICTED: ribosomal RNA large subunit methy... 409 e-140
>ref|XP_002300266.1| predicted protein [Populus trichocarpa] gi|222847524|gb|EEE85071.1|
predicted protein [Populus trichocarpa]
Length = 373
Score = 429 bits (1102), Expect(2) = e-154
Identities = 213/260 (81%), Positives = 233/260 (89%)
Frame = +2
Query: 449 PGGPRSTLCISSQVGCKMGCKFCATGSMGFKSNLSTGEIVEQLVHASRLSPIRNVVFMGM 628
PGGPRSTLCISSQVGCKMGCKFCATGSMGFK+NLS+GEIVEQLVHAS LS IRNVVFMGM
Sbjct: 110 PGGPRSTLCISSQVGCKMGCKFCATGSMGFKNNLSSGEIVEQLVHASCLSQIRNVVFMGM 169
Query: 629 GEPLNNYTALVEAIRVMTASPFQVSPKKITVSTVGIIHAIKKFHGDLPNLNLAVSLHAPV 808
GEPLNNY+ALVEA+R M+ PFQ+SPK+ITVSTVGIIHAI K H DLP LNLAVSLHAPV
Sbjct: 170 GEPLNNYSALVEAVRAMSGVPFQLSPKRITVSTVGIIHAINKLHKDLPGLNLAVSLHAPV 229
Query: 809 QDIRCQIMPAARAFPLERLMDALAEYQKKSQQKIFIEYIMLDGVNDEEQHAHQLGRLLET 988
QD+RCQIMPAARAFPLE+LMDAL YQK S QKIFIEYIMLDGVNDEEQHAHQLG+LLET
Sbjct: 230 QDVRCQIMPAARAFPLEKLMDALQVYQKNSMQKIFIEYIMLDGVNDEEQHAHQLGKLLET 289
Query: 989 FEVVVNLIPFNPIGALSQFGTSGDQRVTRFQTILRSMYNIRTTVRKQMGQDISGACGQLV 1168
F+VVVNLIPFNPIG+LSQF TS +++V RFQ ILR + NIRTTVRKQMGQDISGACGQLV
Sbjct: 290 FDVVVNLIPFNPIGSLSQFRTSSEEKVLRFQKILRGVNNIRTTVRKQMGQDISGACGQLV 349
Query: 1169 IDLSSKKSEGVASLTDIEDL 1228
++L +K +TDIEDL
Sbjct: 350 VNLPDEKKPPNLGVTDIEDL 369
Score = 143 bits (361), Expect(2) = e-154
Identities = 69/91 (75%), Positives = 76/91 (83%)
Frame = +3
Query: 84 SVFDAAFIRTEFDKTGINTHFIPSIWKYVIQNPNCEWREIPSLPSAVYSLLSSKFKPCTS 263
S+FD +R EF+K GINTHFIP IWKYVI+NPNCEW +IP LPSA YSLL SKFK TS
Sbjct: 5 SIFDGPDVRAEFEKAGINTHFIPFIWKYVIKNPNCEWDDIPDLPSAAYSLLRSKFKTSTS 64
Query: 264 IVDSVIDSTDQVTTKLLIKLQNGAFVEAVIM 356
VDSVI+S D VTTKLL+KLQNGAFVEAVIM
Sbjct: 65 SVDSVINSNDGVTTKLLVKLQNGAFVEAVIM 95
>ref|XP_002265287.1| PREDICTED: ribosomal RNA large subunit methyltransferase N [Vitis
vinifera] gi|147817733|emb|CAN60150.1| hypothetical
protein VITISV_044331 [Vitis vinifera]
gi|296083052|emb|CBI22456.3| unnamed protein product
[Vitis vinifera]
Length = 372
Score = 432 bits (1110), Expect(2) = e-150
Identities = 219/263 (83%), Positives = 231/263 (87%), Gaps = 1/263 (0%)
Frame = +2
Query: 449 PGGPRSTLCISSQVGCKMGCKFCATGSMGFKSNLSTGEIVEQLVHASRLSPIRNVVFMGM 628
PGGPRSTLCISSQVGCKMGCKFCATGSMGFKSNLS+GEIVEQLVHAS S IRNVVFMGM
Sbjct: 110 PGGPRSTLCISSQVGCKMGCKFCATGSMGFKSNLSSGEIVEQLVHASHFSQIRNVVFMGM 169
Query: 629 GEPLNNYTALVEAIRVMTASPFQVSPKKITVSTVGIIHAIKKFHGDLPNLNLAVSLHAPV 808
GEPLNNY+ALVEAI +M SPFQ+SPKKITVSTVGIIHAI K DLPNLNLAVSLHAPV
Sbjct: 170 GEPLNNYSALVEAIHIMQGSPFQLSPKKITVSTVGIIHAINKLQSDLPNLNLAVSLHAPV 229
Query: 809 QDIRCQIMPAARAFPLERLMDALAEYQKKSQQKIFIEYIMLDGVNDEEQHAHQLGRLLET 988
QDIRCQIMPAARAFPLE+LMD L YQ S QKIFIEYIMLD VNDEEQHAHQLG+LLET
Sbjct: 230 QDIRCQIMPAARAFPLEKLMDTLRTYQTNSGQKIFIEYIMLDEVNDEEQHAHQLGKLLET 289
Query: 989 FEVVVNLIPFNPIGALSQFGTSGDQRVTRFQTILRSMYNIRTTVRKQMGQDISGACGQLV 1168
F+VVVNLIPFNPIG LS F TS +Q+V RFQ ILR YNIRTTVRKQMGQDISGACGQLV
Sbjct: 290 FQVVVNLIPFNPIGNLSYFKTSSEQKVARFQKILRGTYNIRTTVRKQMGQDISGACGQLV 349
Query: 1169 IDLSSKKSEGVAS-LTDIEDLHL 1234
++ K+S G A LTDIEDLHL
Sbjct: 350 VNQPDKRSTGSAGLLTDIEDLHL 372
Score = 128 bits (321), Expect(2) = e-150
Identities = 61/95 (64%), Positives = 73/95 (76%)
Frame = +3
Query: 72 MTFRSVFDAAFIRTEFDKTGINTHFIPSIWKYVIQNPNCEWREIPSLPSAVYSLLSSKFK 251
M S+FD +R +F GI+ +FIP IWKYV+QNPNC+W +I SLPSA Y LL S FK
Sbjct: 1 MAISSIFDGGELRRQFHSAGISPNFIPFIWKYVVQNPNCDWDDITSLPSAAYPLLRSHFK 60
Query: 252 PCTSIVDSVIDSTDQVTTKLLIKLQNGAFVEAVIM 356
P TS + +VIDS+D VTTKLLIKLQNG+FVEAVIM
Sbjct: 61 PSTSSLHTVIDSSDNVTTKLLIKLQNGSFVEAVIM 95
>ref|XP_004136937.1| PREDICTED: dual-specificity RNA methyltransferase RlmN 1-like
[Cucumis sativus] gi|449495695|ref|XP_004159917.1|
PREDICTED: dual-specificity RNA methyltransferase RlmN
1-like [Cucumis sativus]
Length = 384
Score = 424 bits (1091), Expect(2) = e-146
Identities = 208/263 (79%), Positives = 235/263 (89%), Gaps = 1/263 (0%)
Frame = +2
Query: 449 PGGPRSTLCISSQVGCKMGCKFCATGSMGFKSNLSTGEIVEQLVHASRLSPIRNVVFMGM 628
PGG RSTLCISSQVGCKMGC+FCATG+MGFKSNL++GEIVEQLVHASR S IRN+VFMGM
Sbjct: 122 PGGLRSTLCISSQVGCKMGCRFCATGTMGFKSNLTSGEIVEQLVHASRFSQIRNIVFMGM 181
Query: 629 GEPLNNYTALVEAIRVMTASPFQVSPKKITVSTVGIIHAIKKFHGDLPNLNLAVSLHAPV 808
GEPLNNY++LVEA+R M A PF +SPK+IT+STVGIIHAI K H DLP LNLAVSLHAPV
Sbjct: 182 GEPLNNYSSLVEAVRAMMAPPFLLSPKRITISTVGIIHAINKLHSDLPGLNLAVSLHAPV 241
Query: 809 QDIRCQIMPAARAFPLERLMDALAEYQKKSQQKIFIEYIMLDGVNDEEQHAHQLGRLLET 988
QDIRCQIMPAARAFPL++LMDAL EYQKKSQQKI IEYIMLDGVNDEEQHAHQLG+LLET
Sbjct: 242 QDIRCQIMPAARAFPLQKLMDALQEYQKKSQQKILIEYIMLDGVNDEEQHAHQLGKLLET 301
Query: 989 FEVVVNLIPFNPIGALSQFGTSGDQRVTRFQTILRSMYNIRTTVRKQMGQDISGACGQLV 1168
F+V+VNLIPFNPIG+ SQF TS D++V+ FQ +LR YNIRTT+RKQMGQDISGACGQLV
Sbjct: 302 FQVIVNLIPFNPIGSSSQFKTSSDEKVSVFQQVLRGTYNIRTTIRKQMGQDISGACGQLV 361
Query: 1169 IDLSSKKSEGVASLT-DIEDLHL 1234
+ L ++ S +S+ DIEDLHL
Sbjct: 362 VSLPNRSSSKQSSVVPDIEDLHL 384
Score = 123 bits (308), Expect(2) = e-146
Identities = 65/104 (62%), Positives = 77/104 (74%), Gaps = 11/104 (10%)
Frame = +3
Query: 78 FRSVFDAAFIRTEFDKTGINTHFIPSIWKYVIQNPN----------CEWREI-PSLPSAV 224
FRS FDA +RTEF+K GI T FIP IWK+++ +PN +W ++ PSLPSA
Sbjct: 4 FRSAFDAGELRTEFEKAGIKTTFIPFIWKHLLHDPNNIFNHNLASEFQWEQVVPSLPSAA 63
Query: 225 YSLLSSKFKPCTSIVDSVIDSTDQVTTKLLIKLQNGAFVEAVIM 356
YSLL KFKP TS V SV+DS+DQVTTKLLIKLQ+GAFVEAVIM
Sbjct: 64 YSLLRLKFKPLTSTVHSVVDSSDQVTTKLLIKLQSGAFVEAVIM 107
>ref|NP_188597.1| radical SAM domain-containing protein [Arabidopsis thaliana]
gi|28393068|gb|AAO41968.1| unknown protein [Arabidopsis
thaliana] gi|28827394|gb|AAO50541.1| unknown protein
[Arabidopsis thaliana] gi|332642748|gb|AEE76269.1|
radical SAM domain-containing protein [Arabidopsis
thaliana]
Length = 372
Score = 404 bits (1039), Expect(2) = e-141
Identities = 199/263 (75%), Positives = 228/263 (86%), Gaps = 1/263 (0%)
Frame = +2
Query: 449 PGGPRSTLCISSQVGCKMGCKFCATGSMGFKSNLSTGEIVEQLVHASRLSPIRNVVFMGM 628
PGG RSTLCISSQVGCKMGC FCATG+MGFKSNL++GEIVEQLVHASR++ IRN+VFMGM
Sbjct: 110 PGGIRSTLCISSQVGCKMGCTFCATGTMGFKSNLTSGEIVEQLVHASRIADIRNIVFMGM 169
Query: 629 GEPLNNYTALVEAIRVMTASPFQVSPKKITVSTVGIIHAIKKFHGDLPNLNLAVSLHAPV 808
GEPLNNY A+VEA+RVM PFQ+SPK+IT+STVGI+HAI K H DLP ++LAVSLHAPV
Sbjct: 170 GEPLNNYNAVVEAVRVMLNQPFQLSPKRITISTVGIVHAINKLHNDLPGVSLAVSLHAPV 229
Query: 809 QDIRCQIMPAARAFPLERLMDALAEYQKKSQQKIFIEYIMLDGVNDEEQHAHQLGRLLET 988
Q+IRCQIMPAARAFPL++LMDAL +QK SQQKIFIEYIMLDGVND+EQHAH LG LL+T
Sbjct: 230 QEIRCQIMPAARAFPLQKLMDALQTFQKNSQQKIFIEYIMLDGVNDQEQHAHLLGELLKT 289
Query: 989 FEVVVNLIPFNPIGALSQFGTSGDQRVTRFQTILRSMYNIRTTVRKQMGQDISGACGQLV 1168
F+VV+NLIPFNPIG+ SQF TS Q V+RFQ ILR Y IRTT+RK+MGQDISGACGQLV
Sbjct: 290 FQVVINLIPFNPIGSTSQFETSSIQGVSRFQKILRETYKIRTTIRKEMGQDISGACGQLV 349
Query: 1169 IDLSS-KKSEGVASLTDIEDLHL 1234
++ KK+ G L DIEDL L
Sbjct: 350 VNQPDIKKTPGTVELRDIEDLLL 372
Score = 125 bits (313), Expect(2) = e-141
Identities = 62/95 (65%), Positives = 73/95 (76%)
Frame = +3
Query: 72 MTFRSVFDAAFIRTEFDKTGINTHFIPSIWKYVIQNPNCEWREIPSLPSAVYSLLSSKFK 251
M +SVFDA+ I++EF+ GIN F IWKYVIQNP+C W EIPSLPSA YSLL SKFK
Sbjct: 1 MKLKSVFDASEIKSEFESAGINPKFAIQIWKYVIQNPDCVWDEIPSLPSAAYSLLHSKFK 60
Query: 252 PCTSIVDSVIDSTDQVTTKLLIKLQNGAFVEAVIM 356
TS + S+ S+D T+KLLIKLQNGAFVEAV+M
Sbjct: 61 TLTSSLHSLFHSSDGTTSKLLIKLQNGAFVEAVVM 95
>ref|XP_003558557.1| PREDICTED: ribosomal RNA large subunit methyltransferase N 2-like
[Brachypodium distachyon]
Length = 382
Score = 409 bits (1050), Expect(2) = e-140
Identities = 201/263 (76%), Positives = 233/263 (88%), Gaps = 1/263 (0%)
Frame = +2
Query: 449 PGGPRSTLCISSQVGCKMGCKFCATGSMGFKSNLSTGEIVEQLVHASRLSPIRNVVFMGM 628
PGG RSTLC+SSQVGCKMGC+FCATG+MGFKSNLS+GEIVEQLVHASR S IRNVVFMGM
Sbjct: 120 PGGVRSTLCVSSQVGCKMGCRFCATGTMGFKSNLSSGEIVEQLVHASRYSQIRNVVFMGM 179
Query: 629 GEPLNNYTALVEAIRVMTASPFQVSPKKITVSTVGIIHAIKKFHGDLPNLNLAVSLHAPV 808
GEPLNNYTA+VEAI V+T PFQ+SPK+ITVSTVGIIH+I KF+ DLPN+NLAVSLHAP
Sbjct: 180 GEPLNNYTAVVEAIHVLTGPPFQLSPKRITVSTVGIIHSINKFNNDLPNINLAVSLHAPD 239
Query: 809 QDIRCQIMPAARAFPLERLMDALAEYQKKSQQKIFIEYIMLDGVNDEEQHAHQLGRLLET 988
QDIRCQIMPAARAFPL +LM+AL YQ +S+Q IFIEYIMLDGVND+EQHAHQLG+LLET
Sbjct: 240 QDIRCQIMPAARAFPLGKLMNALQSYQNESKQTIFIEYIMLDGVNDQEQHAHQLGKLLET 299
Query: 989 FEVVVNLIPFNPIGALSQFGTSGDQRVTRFQTILRSMYNIRTTVRKQMGQDISGACGQLV 1168
F+ VVNLIPFNPIG+ S F TS DQ V +FQ +LR +Y+IRTTVR++MGQDI+GACGQLV
Sbjct: 300 FKAVVNLIPFNPIGSSSNFKTSSDQSVKKFQKVLRGVYSIRTTVRQEMGQDIAGACGQLV 359
Query: 1169 IDLSSKKSEGVAS-LTDIEDLHL 1234
+ L ++S G A+ L+DIEDL +
Sbjct: 360 VSLPDERSAGGATLLSDIEDLRI 382
Score = 117 bits (294), Expect(2) = e-140
Identities = 57/93 (61%), Positives = 72/93 (77%), Gaps = 1/93 (1%)
Frame = +3
Query: 81 RSVFDAAFIRTEFDKTGINTHFIPSIWKYVIQNPNC-EWREIPSLPSAVYSLLSSKFKPC 257
RSVFDAA+IR EFD GI+ HFIP IWKYV+QNP C + +PSLP+A Y+LL +KF+P
Sbjct: 13 RSVFDAAYIRAEFDGAGISPHFIPLIWKYVLQNPRCGDLDGVPSLPAAAYALLRNKFRPT 72
Query: 258 TSIVDSVIDSTDQVTTKLLIKLQNGAFVEAVIM 356
TS + + +S D+ TTKLLI+LQNG VEAV+M
Sbjct: 73 TSTLTAAAESKDRTTTKLLIRLQNGESVEAVVM 105