BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes22_contig00012641
         (962 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAA86933.1| oxidosqualene cyclase [Taraxacum officinale]          468   e-130
ref|XP_002309028.1| predicted protein [Populus trichocarpa] gi|2...   426   e-117
dbj|BAA33462.1| Oxidosqualene Cyclase [Panax ginseng]                 416   e-114
emb|CBD47302.1| cycloartenol synthase [Dioscorea zingiberensis]       411   e-112
emb|CAM91422.1| cycloartenol synthase [Dioscorea zingiberensis]       411   e-112

>dbj|BAA86933.1| oxidosqualene cyclase [Taraxacum officinale]
          Length = 758

 Score =  468 bits (1204), Expect = e-130
 Identities = 221/320 (69%), Positives = 246/320 (76%)
 Frame = +3

Query: 3   KLSKGDDDPWVRSINHHIGRQFWEFDPSLGTPEERSRIENVREEYTRNRFNVKHSSDFLM 182
           +LSKGDDDP VRS+NHH+GRQ+WEFDP  GTPEE+S+IE++REE+TRNR NV HSSD LM
Sbjct: 5   QLSKGDDDPEVRSVNHHMGRQYWEFDPHAGTPEEQSQIESMREEFTRNRLNVTHSSDLLM 64

Query: 183 RFQFARENHNEMKKPQVWKAGHXXXXXXXXXXXXXXXXXXXXXFYSTLQGEDGSWPADYX 362
           RFQ   +N   ++K  V + G                      FYS+LQGEDGSWP DY 
Sbjct: 65  RFQLTSKNRGGIEKSHVKEEG-------GDDEVVVKTLKKALKFYSSLQGEDGSWPGDYG 117

Query: 363 XXXXXXXXXXXXXHVMGKIDVILSTEHQTEIRRYIYNHQNVDGGWGLHIEGHSTIFCTAL 542
                        HVMG  D++LS E Q EIRRY+YNHQNVDGGWGLHIEG+ST+  TAL
Sbjct: 118 GPLFLLPGLIIGLHVMGMKDLVLSIEQQNEIRRYLYNHQNVDGGWGLHIEGNSTMLSTAL 177

Query: 543 NYVSLRLLGERTDGGNGAMAKARSWILDHGSVTHMPSWGKFWLSVLGVYEWRGNNPLPPE 722
           NYVSLRLLGE  D G GAM KA+SWIL HGSVTH+PSWGK WLSVLGVYEWRGNNPLPPE
Sbjct: 178 NYVSLRLLGEELDDGKGAMTKAQSWILKHGSVTHIPSWGKLWLSVLGVYEWRGNNPLPPE 237

Query: 723 IWLLPYFLPLHPGRMWCHTRMVYLPMSYLYGKRFVGPITSIVLSLRRELYKTPYNQVNWD 902
            WLLPYFLPLHPGR+WCHTRMVYLPMSYLYGKRFVGPI+SIVLSLRRELY T Y QVNWD
Sbjct: 238 TWLLPYFLPLHPGRLWCHTRMVYLPMSYLYGKRFVGPISSIVLSLRRELYSTLYYQVNWD 297

Query: 903 LARNQCAKEDLYCPHPITQD 962
           LAR QCAK+DLY PHP+ QD
Sbjct: 298 LARKQCAKDDLYYPHPLIQD 317


>ref|XP_002309028.1| predicted protein [Populus trichocarpa] gi|222855004|gb|EEE92551.1|
           predicted protein [Populus trichocarpa]
          Length = 756

 Score =  426 bits (1094), Expect = e-117
 Identities = 195/320 (60%), Positives = 232/320 (72%)
 Frame = +3

Query: 3   KLSKGDDDPWVRSINHHIGRQFWEFDPSLGTPEERSRIENVREEYTRNRFNVKHSSDFLM 182
           KLS+G+D PW++S+N+H+GRQFWEFDP LGTPEER+++EN R E+T++RF  KHSSD LM
Sbjct: 5   KLSEGND-PWLKSVNNHVGRQFWEFDPHLGTPEERAQVENYRNEFTKSRFLTKHSSDLLM 63

Query: 183 RFQFARENHNEMKKPQVWKAGHXXXXXXXXXXXXXXXXXXXXXFYSTLQGEDGSWPADYX 362
           RFQFAREN  EMK P                            FYSTLQ EDG WP DY 
Sbjct: 64  RFQFARENPCEMKLPMA----KVRSEEEITKEVVDTTLRRSLRFYSTLQAEDGFWPGDYG 119

Query: 363 XXXXXXXXXXXXXHVMGKIDVILSTEHQTEIRRYIYNHQNVDGGWGLHIEGHSTIFCTAL 542
                        +V G ++ IL  +H+ E+ RY+YNHQNVDGGWGLHIEG ST+FCT L
Sbjct: 120 GPMFLLPGLVICLYVTGALNTILHNQHRQEMCRYLYNHQNVDGGWGLHIEGSSTMFCTVL 179

Query: 543 NYVSLRLLGERTDGGNGAMAKARSWILDHGSVTHMPSWGKFWLSVLGVYEWRGNNPLPPE 722
           +YV+LRLLGE  DGG+G+M KAR W+LDHG  TH+PSWGK WLSVLGVYEW GNNPL PE
Sbjct: 180 SYVTLRLLGEEMDGGDGSMGKARKWVLDHGGATHIPSWGKMWLSVLGVYEWSGNNPLLPE 239

Query: 723 IWLLPYFLPLHPGRMWCHTRMVYLPMSYLYGKRFVGPITSIVLSLRRELYKTPYNQVNWD 902
           +WLLPY  P HPGR+WC  RMVYLPMSYLYGK+FV   TS+VLS+RRE+Y  PY+Q++WD
Sbjct: 240 LWLLPYLFPAHPGRLWCQCRMVYLPMSYLYGKKFVCSTTSLVLSIRREIYTIPYHQIDWD 299

Query: 903 LARNQCAKEDLYCPHPITQD 962
             RN CAKEDLY PHP+ QD
Sbjct: 300 QTRNLCAKEDLYYPHPLLQD 319


>dbj|BAA33462.1| Oxidosqualene Cyclase [Panax ginseng]
          Length = 780

 Score =  416 bits (1068), Expect = e-114
 Identities = 196/330 (59%), Positives = 234/330 (70%), Gaps = 10/330 (3%)
 Frame = +3

Query: 3   KLSKGDDDPWVRSINHHIGRQFWEFDPSLGTPEERSRIENVREEYTRNRFNVKHSSDFLM 182
           KL     DP ++S+N+HIGRQFWEFDP+LGTPEER+ I+ +R+++  NRF VKHSSD LM
Sbjct: 3   KLILSQGDPGLKSVNNHIGRQFWEFDPNLGTPEERAHIDKLRQQFHNNRFRVKHSSDLLM 62

Query: 183 RFQFARENHNEMKKPQVWKAGHXXXXXXXXXXXXXXXXXXXXX--FYSTLQGEDGSWPAD 356
           R+QF RE   ++      K+G                        FYST+Q +DG WP D
Sbjct: 63  RYQFEREKSRKLGDDDEVKSGSEGEITTSSSGVEGVKMALRRALKFYSTIQADDGHWPGD 122

Query: 357 YXXXXXXXXXXXXXXHVMGKIDVILSTEHQTEIRRYIYNHQNVDGGWGLHIEGHSTIFCT 536
           Y              +VMG +D IL+ EHQ E+ RYIYNHQNVDGGWGLHIEG ST+ CT
Sbjct: 123 YGGPLFLLPGLVIGLYVMGVMDTILAKEHQREMCRYIYNHQNVDGGWGLHIEGCSTMLCT 182

Query: 537 ALNYVSLRLL--GERTD------GGNGAMAKARSWILDHGSVTHMPSWGKFWLSVLGVYE 692
           ALNY++LRLL  G+  +         G++ KAR WI+DHG  T++PSWGKFWLS+LGVYE
Sbjct: 183 ALNYITLRLLIRGDEEEEIRDEAANGGSLEKARRWIIDHGGATYIPSWGKFWLSILGVYE 242

Query: 693 WRGNNPLPPEIWLLPYFLPLHPGRMWCHTRMVYLPMSYLYGKRFVGPITSIVLSLRRELY 872
           W GNNPLPPE+WLLPYFLPLHPGRMW H RMVYLPMSYLYG+RFVGPI S VLSLRRELY
Sbjct: 243 WSGNNPLPPEMWLLPYFLPLHPGRMWNHCRMVYLPMSYLYGRRFVGPINSTVLSLRRELY 302

Query: 873 KTPYNQVNWDLARNQCAKEDLYCPHPITQD 962
             PY+Q+NWDLARNQCA+EDLY PHP+ QD
Sbjct: 303 THPYHQINWDLARNQCAQEDLYYPHPLIQD 332


>emb|CBD47302.1| cycloartenol synthase [Dioscorea zingiberensis]
          Length = 759

 Score =  411 bits (1057), Expect = e-112
 Identities = 187/320 (58%), Positives = 231/320 (72%)
 Frame = +3

Query: 3   KLSKGDDDPWVRSINHHIGRQFWEFDPSLGTPEERSRIENVREEYTRNRFNVKHSSDFLM 182
           K+++G + PW+R+ N+H+GRQ WEFDP+L TPEE + +E  RE + ++RF  KHSSD LM
Sbjct: 5   KIAEGGN-PWLRTTNNHVGRQVWEFDPNLRTPEELAEVERAREAFHQHRFEKKHSSDLLM 63

Query: 183 RFQFARENHNEMKKPQVWKAGHXXXXXXXXXXXXXXXXXXXXXFYSTLQGEDGSWPADYX 362
           R QFA+EN  E+  PQV                           +STLQ  DG WP DY 
Sbjct: 64  RLQFAKENPLELTLPQV----KVRDDEDVTEEAVTTTVRRAISRHSTLQAHDGHWPGDYG 119

Query: 363 XXXXXXXXXXXXXHVMGKIDVILSTEHQTEIRRYIYNHQNVDGGWGLHIEGHSTIFCTAL 542
                        +V G ++ +LS EHQ EI RY+YNHQN DGGWGLHIEGHST+F +AL
Sbjct: 120 GPMFLMPGLVIALYVTGALNTVLSPEHQREICRYLYNHQNKDGGWGLHIEGHSTMFGSAL 179

Query: 543 NYVSLRLLGERTDGGNGAMAKARSWILDHGSVTHMPSWGKFWLSVLGVYEWRGNNPLPPE 722
            Y++LRLLGE+T+GG+GAM + R WILDHG  T + SWGKFWLSVLGV++W GNNPLPPE
Sbjct: 180 TYITLRLLGEKTEGGDGAMQRGRKWILDHGGATFITSWGKFWLSVLGVFDWSGNNPLPPE 239

Query: 723 IWLLPYFLPLHPGRMWCHTRMVYLPMSYLYGKRFVGPITSIVLSLRRELYKTPYNQVNWD 902
           +W+LPYFLP+HPGRMWCH RMVYLPMSY+YGKRFVGPIT ++ SLR+ELY   Y+Q+NW+
Sbjct: 240 VWMLPYFLPIHPGRMWCHCRMVYLPMSYIYGKRFVGPITPLIQSLRKELYNLSYDQINWN 299

Query: 903 LARNQCAKEDLYCPHPITQD 962
           LARNQCAKEDLY PHP+ QD
Sbjct: 300 LARNQCAKEDLYYPHPLVQD 319


>emb|CAM91422.1| cycloartenol synthase [Dioscorea zingiberensis]
          Length = 759

 Score =  411 bits (1057), Expect = e-112
 Identities = 187/320 (58%), Positives = 231/320 (72%)
 Frame = +3

Query: 3   KLSKGDDDPWVRSINHHIGRQFWEFDPSLGTPEERSRIENVREEYTRNRFNVKHSSDFLM 182
           K+++G + PW+R+ N+H+GRQ WEFDP+L TPEE + +E  RE + ++RF  KHSSD LM
Sbjct: 5   KIAEGGN-PWLRTTNNHVGRQVWEFDPNLRTPEELAEVERAREAFHQHRFEKKHSSDLLM 63

Query: 183 RFQFARENHNEMKKPQVWKAGHXXXXXXXXXXXXXXXXXXXXXFYSTLQGEDGSWPADYX 362
           R QFA+EN  E+  PQV                           +STLQ  DG WP DY 
Sbjct: 64  RLQFAKENPLELTLPQV----KVRDDEDVTEEAVTTTVRRAISRHSTLQAHDGHWPGDYG 119

Query: 363 XXXXXXXXXXXXXHVMGKIDVILSTEHQTEIRRYIYNHQNVDGGWGLHIEGHSTIFCTAL 542
                        +V G ++ +LS EHQ EI RY+YNHQN DGGWGLHIEGHST+F +AL
Sbjct: 120 GPMFLMPGLVIALYVTGALNTVLSPEHQREICRYLYNHQNKDGGWGLHIEGHSTMFGSAL 179

Query: 543 NYVSLRLLGERTDGGNGAMAKARSWILDHGSVTHMPSWGKFWLSVLGVYEWRGNNPLPPE 722
            Y++LRLLGE+T+GG+GAM + R WILDHG  T + SWGKFWLSVLGV++W GNNPLPPE
Sbjct: 180 TYITLRLLGEKTEGGDGAMQRGRKWILDHGGATFITSWGKFWLSVLGVFDWSGNNPLPPE 239

Query: 723 IWLLPYFLPLHPGRMWCHTRMVYLPMSYLYGKRFVGPITSIVLSLRRELYKTPYNQVNWD 902
           +W+LPYFLP+HPGRMWCH RMVYLPMSY+YGKRFVGPIT ++ SLR+ELY   Y+Q+NW+
Sbjct: 240 VWMLPYFLPIHPGRMWCHCRMVYLPMSYIYGKRFVGPITPLIQSLRKELYNLSYDQINWN 299

Query: 903 LARNQCAKEDLYCPHPITQD 962
           LARNQCAKEDLY PHP+ QD
Sbjct: 300 LARNQCAKEDLYYPHPLVQD 319