BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes22_contig00012568
         (2862 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Viti...   961   0.0  
gb|AAN15318.1| isoamylase isoform 2 [Solanum tuberosum]               940   0.0  
ref|XP_002533079.1| isoamylase, putative [Ricinus communis] gi|2...   931   0.0  
ref|XP_004136188.1| PREDICTED: isoamylase 2, chloroplastic-like ...   891   0.0  
ref|XP_004164565.1| PREDICTED: LOW QUALITY PROTEIN: isoamylase 2...   891   0.0  

>ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Vitis vinifera]
          Length = 882

 Score =  961 bits (2485), Expect = 0.0
 Identities = 495/884 (55%), Positives = 623/884 (70%), Gaps = 40/884 (4%)
 Frame = -1

Query: 2775 MAMLSLSSATQPPYLTCRTTNLSKLIVADSCKYGRRVAQSLGRMDMERSLVFGEVAKNMI 2596
            M  L  S   +P  + C   + SKL+ A     G +VA  L +MD+ER  + G VA+N  
Sbjct: 1    MTTLIPSLVIRPCCVNCGARDSSKLVAATHYICGNKVAHGLEKMDLERKEILGVVAQNAT 60

Query: 2595 KGSCHDLGVGVLAASTLPVIQTVDKLST-----------YLFRTEKGGHVKVITRLKDGK 2449
            +    ++   V A S + + +T ++ ST           YLF TE GG VKVI   K+ K
Sbjct: 61   RNCFRNINWKVSATSHIAIEETENRYSTTESEELESRLNYLFLTEIGGQVKVIVGKKNKK 120

Query: 2448 YGVDVEVSSLQLCGNDDDVIMSWGIFRXXXXXXXXXXXXXS------GIFETPFVNKSLN 2287
            Y V +EVSSLQL  +D+ +I+SWG+FR                       E PF+ +S  
Sbjct: 121  YIVSIEVSSLQLYNSDNKLILSWGVFRSNSSCFMPVDFQNLVPEVGSNTTEIPFMERSSG 180

Query: 2286 KLSVELEFDASLVPFYVSFLLKAPFSGE--KLEIRSHRKTNFCFPVGIRSGYPAPLGLSY 2113
              +++L+F+A+  PFY+SFLLK+    +    +IRSHRKTNFC PVG   GYPAPLGLS+
Sbjct: 181  SFALKLDFEANHAPFYLSFLLKSTLDTDLSSSDIRSHRKTNFCIPVGFGRGYPAPLGLSF 240

Query: 2112 TADGSINFALFSRNAKSVILCLFDDSSLEEPTLEIDLDPYINRSGDIWHASMDSAMNFVS 1933
            ++DGS NFA FSRNA  V+LCL+D +S ++P LEIDLDPY+NR+GDIWHASM+S  +FVS
Sbjct: 241  SSDGSPNFAFFSRNAGGVVLCLYDGTS-DKPALEIDLDPYVNRTGDIWHASMESVGSFVS 299

Query: 1932 YGYRCRNG-AQDNSQA---SCILLDPYAKVIGENF-----------LGEICSELAFDWSG 1798
            YGYRC+    QD+ +      + LDPYAK+I  +F           LGE+  E AF+W+ 
Sbjct: 300  YGYRCKEANLQDSGETLHTEHVHLDPYAKLIRNSFSDDHGLKPQPRLGELQKEPAFNWND 359

Query: 1797 DVHPRLSMEKLMVYRLNVMDFTKDASSNLPNEIRGTFSGVTEKLHHFKDLGVNAILLEPI 1618
            DVHP + MEKL+VYRLNVM FTKD SS + +++ GTFSG+ EKLHHFKDLGVNA+LLEPI
Sbjct: 360  DVHPYIPMEKLVVYRLNVMHFTKDESSQVASDLAGTFSGLMEKLHHFKDLGVNAVLLEPI 419

Query: 1617 VPFDKQVGPYFPCHFFSPQNSFGPHGGSTSTIKSMKEMVKRLHANGIEILLEVVLTHTCD 1438
              FD+Q GPYFP HFFSP N +GP  G  STI S+KEMVKRLHANGIE+LLEVV THT +
Sbjct: 420  FSFDEQKGPYFPFHFFSPMNVYGPSSGPVSTINSVKEMVKRLHANGIEVLLEVVFTHTAE 479

Query: 1437 DAPLTKIDRPSYYLGKEGDDLATKNSLNCAHTVVQQLILDSLRHWVIEYHIDGFCFINAS 1258
               L  ID   YY      DL  +N+LNC +++VQQ+I+DSLR+WV E+H+DGFCFINAS
Sbjct: 480  SGALQGIDDSCYYYVNGDADLGIRNALNCNYSIVQQMIVDSLRYWVTEFHVDGFCFINAS 539

Query: 1257 SMLRGFNGENLSRPPLIEAIAFDPILSKTKIIADSFDPFHKSAKEIQFPHWKRWAEMNTK 1078
            S+LRGF+GE LSRPPL+E IAFDP+LSKTKIIAD +DP +   KEI+FPHWKRWAE+NT+
Sbjct: 540  SLLRGFHGEYLSRPPLVETIAFDPLLSKTKIIADCWDPRNMLPKEIRFPHWKRWAEVNTR 599

Query: 1077 FCSDARNYLRGKTLLSNLATRLCGSGDIFLNGRGPAFSFNFVSRNHGLSLVDLVSFSS-- 904
            FC+D RN+LRG+  LS+ ATRLCGSGDIF++GRGPAFSFNF ++N GL LVDLVSFSS  
Sbjct: 600  FCNDVRNFLRGEG-LSDFATRLCGSGDIFMDGRGPAFSFNFTTKNFGLPLVDLVSFSSSE 658

Query: 903  -ATELSWNCGEEGATKKKGVLETRLKQIRNFLFILYVSLGVPVLNMGDECGRSSGGSLEH 727
             A+ELSWNCG+EG T K  VLE RLKQIRNFLFILYVSLGVP+LNMGDECG+SSGGS  +
Sbjct: 659  LASELSWNCGDEGPTNKTTVLERRLKQIRNFLFILYVSLGVPILNMGDECGQSSGGSPAY 718

Query: 726  SDRKPFNWKALETGFAIQTTQFISFLSTLKIRRSDLLQSREFLKVENIDWHGTNLSPPDW 547
             DRKPFNW +++TGF IQT QFISFLS+L+ RRSDLLQ R FLK E+IDWHG++ SPP W
Sbjct: 719  GDRKPFNWNSVKTGFGIQTIQFISFLSSLRSRRSDLLQRRSFLKEESIDWHGSDQSPPRW 778

Query: 546  EDPTSKFLAVSLXXXXXXXXDENQ---LNGDMFVAFNGGDDPMIATLPPPASNMVWLRLV 376
            +DP+SKFLA++L          ++   + GD+F+AFN  D  +   LPPP + MVW RLV
Sbjct: 779  DDPSSKFLAMTLKAENMEGQLPSESSSIKGDLFIAFNTADRSVKVILPPPPTGMVWHRLV 838

Query: 375  DTALRYPGFFSTAGDPTPEKIPGSPVYEMAPHSCVLLEARSVDG 244
            DTAL +PGFF+  G+   +K  G   Y+M  HSC L EA ++DG
Sbjct: 839  DTALPFPGFFTADGEAILKKKSGLVTYKMESHSCALFEANTLDG 882


>gb|AAN15318.1| isoamylase isoform 2 [Solanum tuberosum]
          Length = 878

 Score =  940 bits (2429), Expect = 0.0
 Identities = 485/876 (55%), Positives = 613/876 (69%), Gaps = 24/876 (2%)
 Frame = -1

Query: 2775 MAMLSLSSATQPPYLTCRTTNLSKLIVADSCKYGRRVAQSLGRMDMERSLVFGEVAKNMI 2596
            MA   +  A     L+  +T  +KL+ + S   G+ V  SL ++++E     G    N  
Sbjct: 1    MATSPIQLAVHSRLLSYGSTESTKLVPSSSGNRGKIVC-SLRKLELEDMNFSGIGRNNDQ 59

Query: 2595 KGSCHDLGVGVLAASTLPVIQTVDKLSTYLFRTEKGGHVKVITRLKDGKYGVDVEVSSLQ 2416
            +          L+AS + ++ +  ++ TYLFRT+ GG VKV+    +GKY V VEV  L+
Sbjct: 60   EAPRRAHRRKALSASRISLVPSAKRVPTYLFRTDIGGQVKVLVERTNGKYKVLVEVLPLE 119

Query: 2415 LCGNDDDVIMSWGIFRXXXXXXXXXXXXXSGI------FETPFVNKSLNKLSVELEFDAS 2254
            L     +++M WG+FR              G        ETPFV     K++VEL+F+AS
Sbjct: 120  LSYAHSELVMVWGLFRSDASCFMPLDLNRRGADGKSSTVETPFVQGPSGKVTVELDFEAS 179

Query: 2253 LVPFYVSFLLKAPFSG--EKLEIRSHRKTNFCFPVGIRSGYPAPLGLSYTADGSINFALF 2080
            L PFY+SF +K+      E  EIRSHR TNF  PVG+ SG+PAPLG+S+  DGS+NFALF
Sbjct: 180  LAPFYISFYMKSQLVSDMENSEIRSHRNTNFVVPVGLSSGHPAPLGISFQPDGSVNFALF 239

Query: 2079 SRNAKSVILCLFDDSSLEEPTLEIDLDPYINRSGDIWHASMDSAMNFVSYGYRCRNGAQD 1900
            SR+A+SV+LCL+DD S+E+P+LEIDLDPYINRSGDIWHA++D ++ F +YGYRC+  A  
Sbjct: 240  SRSARSVVLCLYDDISVEKPSLEIDLDPYINRSGDIWHAALDCSLPFKTYGYRCK--ATT 297

Query: 1899 NSQASCILLDPYAKVIGE------------NFLGEICSELAFDWSGDVHPRLSMEKLMVY 1756
            + +   +LLDPYAKVI               +LGE+C E  +DWSGDV P L MEKL++Y
Sbjct: 298  SGKGELVLLDPYAKVIRRVIPRQGGSEIRPKYLGELCLEPGYDWSGDVPPSLPMEKLIIY 357

Query: 1755 RLNVMDFTKDASSNLPNEIRGTFSGVTEKLHHFKDLGVNAILLEPIVPFDKQVGPYFPCH 1576
            RLNV  FTKD SS LP+++ GTFSG++EK HHFKDLGVNA+LLEPI PFD+Q GPYFP H
Sbjct: 358  RLNVTQFTKDKSSKLPDDLAGTFSGISEKWHHFKDLGVNAMLLEPIFPFDEQKGPYFPWH 417

Query: 1575 FFSPQNSFGPHGGSTSTIKSMKEMVKRLHANGIEILLEVVLTHTCDDAPLTKIDRPSYYL 1396
            FFSP N +GP G   S IKSMK+MVK+LHANGIE+ LEVV THT +DAPL  +D  SY +
Sbjct: 418  FFSPGNMYGPSGDPLSAIKSMKDMVKKLHANGIEVFLEVVFTHTAEDAPLMNVDNFSYCI 477

Query: 1395 GKEGDDLATKNSLNCAHTVVQQLILDSLRHWVIEYHIDGFCFINASSMLRGFNGENLSRP 1216
             K G  L  +N+LNC + +VQQ+ILD LRHWVIE+HIDGF F+NASS+LRGFNGE LSRP
Sbjct: 478  -KGGQYLNIQNALNCNYPIVQQMILDCLRHWVIEFHIDGFVFVNASSLLRGFNGEILSRP 536

Query: 1215 PLIEAIAFDPILSKTKIIADSFDPFHKSAKEIQFPHWKRWAEMNTKFCSDARNYLRGKTL 1036
            PL+EAIAFDPILSK K+IAD+++P    +KE  FPHW+RWAE+N +FC D R++LRG+ L
Sbjct: 537  PLVEAIAFDPILSKVKMIADNWNPLTNDSKENLFPHWRRWAEINMRFCDDIRDFLRGEGL 596

Query: 1035 LSNLATRLCGSGDIFLNGRGPAFSFNFVSRNHGLSLVDLVSFSS---ATELSWNCGEEGA 865
            LSNLATRLCGSGDIF  GRGPAFSFN+++RN GL+LVDLVSFSS   A+ELSWNCG+EGA
Sbjct: 597  LSNLATRLCGSGDIFAGGRGPAFSFNYIARNSGLTLVDLVSFSSNEVASELSWNCGQEGA 656

Query: 864  TKKKGVLETRLKQIRNFLFILYVSLGVPVLNMGDECGRSSGGSLEHSDRKPFNWKALETG 685
            T    VLE RLKQ+RNFLFIL++SLGVPVLNMGDECG+SSGG   +  RK   W  L+TG
Sbjct: 657  TTNNIVLERRLKQVRNFLFILFISLGVPVLNMGDECGQSSGGPPAYDARKSLGWNTLKTG 716

Query: 684  FAIQTTQFISFLSTLKIRRSDLLQSREFLKVENIDWHGTNLSPPDWEDPTSKFLAVSL-X 508
            F  Q  QFISFLS L++RRSDLLQ R FLK ENI WHG++ SPP W+ P+SKFLA++L  
Sbjct: 717  FGTQIAQFISFLSNLRMRRSDLLQKRTFLKEENIQWHGSDQSPPKWDGPSSKFLAMTLKA 776

Query: 507  XXXXXXXDENQLNGDMFVAFNGGDDPMIATLPPPASNMVWLRLVDTALRYPGFFSTAGDP 328
                     + + GD+FVAFNG  D  I  LPPP ++MVW RLVDTAL +PGFF   G P
Sbjct: 777  DAEVSQTLVSDIVGDLFVAFNGAGDSEIVILPPPPTDMVWHRLVDTALPFPGFFDEKGTP 836

Query: 327  TPEKIPGSPVYEMAPHSCVLLEARSVDG*HSTYRKE 220
              +++     YEM  HSC+L EA+ +    S+ RK+
Sbjct: 837  VEDEL---VAYEMKSHSCLLFEAQRLAEIDSSKRKK 869


>ref|XP_002533079.1| isoamylase, putative [Ricinus communis] gi|223527143|gb|EEF29318.1|
            isoamylase, putative [Ricinus communis]
          Length = 872

 Score =  931 bits (2407), Expect = 0.0
 Identities = 481/884 (54%), Positives = 602/884 (68%), Gaps = 40/884 (4%)
 Frame = -1

Query: 2775 MAMLSLSSATQPPYLTCRTTNLSKLIVADSCKYGRRVAQSLGRMDMERSLVFGEVAKNMI 2596
            MA +  S A +P Y  C  T  SKL V  +  +GR V     R+  +R         +  
Sbjct: 1    MATIIPSFAIRPCYCVCGATESSKLAVTGNKDFGRLVLDQ--RLFNQRL--------HFW 50

Query: 2595 KGSCH-DLGVGVLAASTLPVIQT------------VDKLSTYLFRTEKGGHVKVITRLKD 2455
            K S H  L   V +A+ +PV QT            ++K+STYLFRT+ GGHVKV+ R K+
Sbjct: 51   KQSPHWSLDSRVCSAARVPVQQTELRFSTSAPLDELNKVSTYLFRTQFGGHVKVLVRKKN 110

Query: 2454 GKYGVDVEVSSLQLCGNDDDVIMSWGIFRXXXXXXXXXXXXXSGI----FETPFVNKSLN 2287
             KY V +EVSSL+L   D  +++ WGI+R                     +T  V  S  
Sbjct: 111  AKYAVYIEVSSLELGTTDYRLMLIWGIYRSDSSCFMPLDSQNFAPNARKMDTALVQNSFG 170

Query: 2286 KLSVELEFDASLVPFYVSFLLKAPFSGEK--LEIRSHRKTNFCFPVGIRSGYPAPLGLSY 2113
              ++ELEF+    PFY+SFLLK+  + +   LEI++H+  NFC P+G  SG P+PLGLS+
Sbjct: 171  TFALELEFEPKQTPFYLSFLLKSKLNTDASGLEIKNHKNANFCVPIGFNSGDPSPLGLSF 230

Query: 2112 TADGSINFALFSRNAKSVILCLFDDSSLEEPTLEIDLDPYINRSGDIWHASMDSAMNFVS 1933
            + DGS+NFA FSRN + ++LCL+DDS+ ++P LE+DLDPY+NR+GD+WHAS++ A  F S
Sbjct: 231  STDGSMNFAFFSRNVEGLVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASLEGAWTFTS 290

Query: 1932 YGYRCRN----GAQDNSQASCILLDPYAKVIGEN-----------FLGEICSELAFDWSG 1798
            YGYRC+     G        C+LLDPYA+VI  +           +LG +C E AF+W  
Sbjct: 291  YGYRCKGAILQGNTSKVDMECVLLDPYARVIASSMTDHGSRLSAKYLGRLCEEPAFEWGS 350

Query: 1797 DVHPRLSMEKLMVYRLNVMDFTKDASSNLPNEIRGTFSGVTEKLHHFKDLGVNAILLEPI 1618
            D+ P L+MEKL+VYRLNV  FT+  S  L ++I GTF+G+ EK+ HF++LGVNA+LLEPI
Sbjct: 351  DIRPNLAMEKLIVYRLNVKRFTEHKSGKLYSDIAGTFAGLIEKMDHFRNLGVNAVLLEPI 410

Query: 1617 VPFDKQVGPYFPCHFFSPQNSFGPHGGSTSTIKSMKEMVKRLHANGIEILLEVVLTHTCD 1438
             PFD+Q GPYFP HFFSP N +GP GGS S I SMKEMVK LHAN IE+LLEVV THT +
Sbjct: 411  FPFDEQKGPYFPYHFFSPSNIYGPSGGSISAITSMKEMVKELHANRIEVLLEVVFTHTAE 470

Query: 1437 DAPLTKIDRPSYYLGKEGDDLATKNSLNCAHTVVQQLILDSLRHWVIEYHIDGFCFINAS 1258
               L  ID  SYY  K   D  ++N+LNC + +VQ++ILDSL+HWV E+HIDGFCFINAS
Sbjct: 471  GGALQGIDDFSYYYTKSSMD--SRNALNCNYPIVQRMILDSLQHWVTEFHIDGFCFINAS 528

Query: 1257 SMLRGFNGENLSRPPLIEAIAFDPILSKTKIIADSFDPFHKSAKEIQFPHWKRWAEMNTK 1078
            ++L GF+GE+LSRPPL+EAIAFDPILSKTKIIAD + P H+  KE  FPHWKRWAE+N K
Sbjct: 529  ALLTGFHGEHLSRPPLVEAIAFDPILSKTKIIADPWHPEHRIPKETCFPHWKRWAEINPK 588

Query: 1077 FCSDARNYLRGKTLLSNLATRLCGSGDIFLNGRGPAFSFNFVSRNHGLSLVDLVSFSS-- 904
            FC D RN+LRG++LL +LATRLCGSGDIF NGRGPAFSFN+++RN GL LVDLVSFS   
Sbjct: 589  FCIDVRNFLRGESLLGDLATRLCGSGDIFSNGRGPAFSFNYIARNSGLPLVDLVSFSGGE 648

Query: 903  -ATELSWNCGEEGATKKKGVLETRLKQIRNFLFILYVSLGVPVLNMGDECGRSSGGSLEH 727
              +ELSWNCGEEG T K  VLE RLKQIRN+LFILYVSLGVPVLNMGDECG+SS GS+ +
Sbjct: 649  LGSELSWNCGEEGPTNKTAVLERRLKQIRNYLFILYVSLGVPVLNMGDECGQSSRGSISY 708

Query: 726  SDRKPFNWKALETGFAIQTTQFISFLSTLKIRRSDLLQSREFLKVENIDWHGTNLSPPDW 547
             DRKPF+W AL T F  Q TQFISFLS+L++RRSDLLQ R FLK ENIDWHG + SPP W
Sbjct: 709  GDRKPFDWNALSTSFGNQMTQFISFLSSLRMRRSDLLQKRNFLKEENIDWHGNDQSPPRW 768

Query: 546  EDPTSKFLAVSLXXXXXXXXDENQ---LNGDMFVAFNGGDDPMIATLPPPASNMVWLRLV 376
            EDPT KFLA++L          ++   + GD+F+AFN         LPP    M+W RLV
Sbjct: 769  EDPTCKFLAMTLKIDKAESQLSSEPSNIKGDLFMAFNAAGHAESVILPPVPEGMIWRRLV 828

Query: 375  DTALRYPGFFSTAGDPTPEKIPGSPVYEMAPHSCVLLEARSVDG 244
            DTAL +PGFFS  G+P  E+I G   Y+M  HSC L EA  +DG
Sbjct: 829  DTALPFPGFFSEDGEPVVEQIAGLIAYKMNSHSCTLFEAGIMDG 872


>ref|XP_004136188.1| PREDICTED: isoamylase 2, chloroplastic-like [Cucumis sativus]
          Length = 885

 Score =  891 bits (2303), Expect = 0.0
 Identities = 465/883 (52%), Positives = 589/883 (66%), Gaps = 45/883 (5%)
 Frame = -1

Query: 2760 LSSATQPPYLT--CRTTNLSKLIVADSCKYGRRVAQSLGRMDMERSLVFGEVAKNMIKGS 2587
            LSS    P+    C  T   KL  +D   YG++     G+MD  R L  GE     +K S
Sbjct: 5    LSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGAVKSS 64

Query: 2586 CHDLGVGVLAASTLPVIQTVDKL------------STYLFRTEKGGHVKVITRLKDGKYG 2443
              +L     A S + V ++  +L            +TYLFRTE G  V V    K   + 
Sbjct: 65   HRNLSK-TYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGSTFT 123

Query: 2442 VDVEVSSLQLCGNDDDVIMSWGIFRXXXXXXXXXXXXXS-----GIFETPFVNKSLNKLS 2278
            V++EV S+QL   D+ +++SWG++R                   G  ETPFV  S  K S
Sbjct: 124  VNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEGKFS 183

Query: 2277 VELEFDASLVPFYVSFLLKAPFSGEK--LEIRSHRKTNFCFPVGIRSGYPAPLGLSYTAD 2104
            VELEFDA   PFY+SF+LK P   +    EIRSH+KT+F  PVG   GYP+PLGLS + D
Sbjct: 184  VELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSISGD 243

Query: 2103 GSINFALFSRNAKSVILCLFDDSSLEEPTLEIDLDPYINRSGDIWHASMDSAMNFVSYGY 1924
            GS+NF++FS +A+S++LCL++DS+ E+P LE+DLDPYINRSG+IWHAS + A  FVSYGY
Sbjct: 244  GSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVSYGY 303

Query: 1923 RCRNGA----QDNSQASCILLDPYAKVIGEN--------------FLGEICSELAFDWSG 1798
            +C+       QD  + S I++DPYAK++  +              FLG+I     FDW G
Sbjct: 304  QCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFDWDG 363

Query: 1797 DVHPRLSMEKLMVYRLNVMDFTKDASSNLPNEIRGTFSGVTEKLHHFKDLGVNAILLEPI 1618
            +VHP L MEKL VYRLNV  FT D SS LP +I GTFSG+T+KL HFK+LGVNA+LLEPI
Sbjct: 364  EVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLLEPI 423

Query: 1617 VPFDKQVGPYFPCHFFSPQNSFGPHGGSTSTIKSMKEMVKRLHANGIEILLEVVLTHTCD 1438
              FD++ GPYFP HFFSP N++GP G S S I SMKEMVK LHANG+E++LEVV THT  
Sbjct: 424  FQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTHTSG 483

Query: 1437 DAPLTKIDRPSYYLGKEGDDLATKNSLNCAHTVVQQLILDSLRHWVIEYHIDGFCFINAS 1258
            +  L  ID  SYY      +L  K++LNC + +VQQL+LDSLR+WV E+H+DGFCF+NAS
Sbjct: 484  NGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFVNAS 543

Query: 1257 SMLRGFNGENLSRPPLIEAIAFDPILSKTKIIADSFDPFHKSAKEIQFPHWKRWAEMNTK 1078
             +LRG +GE LSRPP +EAIAFDP+LSKTK++AD +DP    +KE +FPHWKRWAE+N+K
Sbjct: 544  FLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEVNSK 603

Query: 1077 FCSDARNYLRGKTLLSNLATRLCGSGDIFLNGRGPAFSFNFVSRNHGLSLVDLVSFSS-- 904
            FCSD R++ RG+ L+S+LATRLCGSGD+F +GRGPAFSFNF++RN GL LVDLVSFS+  
Sbjct: 604  FCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFSNSN 663

Query: 903  -ATELSWNCGEEGATKKKGVLETRLKQIRNFLFILYVSLGVPVLNMGDECGRSSGGSLEH 727
             A+ELSWNCGEEG T    VLE RLKQIRNF+F+L+VSLGVPVLNMGDECG+SSGGS+  
Sbjct: 664  LASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSVAF 723

Query: 726  SDRKPFNWKALETGFAIQTTQFISFLSTLKIRRSDLLQSREFLKVENIDWHGTNLSPPDW 547
            +D++ FNW  L+T F  QTTQFI+FLS+ + RR DL Q+R FLK ENIDW   N SPP W
Sbjct: 724  NDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSPPQW 783

Query: 546  EDPTSKFLAVSL--XXXXXXXXDEN-QLNGDMFVAFNGGDDPMIATLPPPASNMVWLRLV 376
            ED + KFLAV L           EN +   ++F+ FN  D      LP P     W R+V
Sbjct: 784  EDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWFRVV 843

Query: 375  DTALRYPGFFSTAGDPTPEKIPGSPVYEMAPHSCVLLEARSVD 247
            DTAL +PGFFS+ G+  P  + GS  YE+  HSC L EA+S +
Sbjct: 844  DTALPFPGFFSSDGELVP--MTGSVTYEIQAHSCALFEAKSAN 884


>ref|XP_004164565.1| PREDICTED: LOW QUALITY PROTEIN: isoamylase 2, chloroplastic-like
            [Cucumis sativus]
          Length = 885

 Score =  891 bits (2302), Expect = 0.0
 Identities = 465/883 (52%), Positives = 589/883 (66%), Gaps = 45/883 (5%)
 Frame = -1

Query: 2760 LSSATQPPYLT--CRTTNLSKLIVADSCKYGRRVAQSLGRMDMERSLVFGEVAKNMIKGS 2587
            LSS    P+    C  T   KL  +D   YG++     G+MD  R L  GE     +K S
Sbjct: 5    LSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGAVKSS 64

Query: 2586 CHDLGVGVLAASTLPVIQTVDKL------------STYLFRTEKGGHVKVITRLKDGKYG 2443
              +L     A S + V ++  +L            +TYLFRTE G  V V    K   + 
Sbjct: 65   HRNLSK-TYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGSTFT 123

Query: 2442 VDVEVSSLQLCGNDDDVIMSWGIFRXXXXXXXXXXXXXS-----GIFETPFVNKSLNKLS 2278
            V++EV S+QL   D+ +++SWG++R                   G  ETPFV  S  K S
Sbjct: 124  VNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEGKFS 183

Query: 2277 VELEFDASLVPFYVSFLLKAPFSGEK--LEIRSHRKTNFCFPVGIRSGYPAPLGLSYTAD 2104
            VELEFDA   PFY+SF+LK P   +    EIRSH+KT+F  PVG   GYP+PLGLS + D
Sbjct: 184  VELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSISGD 243

Query: 2103 GSINFALFSRNAKSVILCLFDDSSLEEPTLEIDLDPYINRSGDIWHASMDSAMNFVSYGY 1924
            GS+NF++FS +A+S++LCL++DS+ E+P LE+DLDPYINRSG+IWHAS + A  FVSYGY
Sbjct: 244  GSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVSYGY 303

Query: 1923 RCRNGA----QDNSQASCILLDPYAKVIGEN--------------FLGEICSELAFDWSG 1798
            +C+       QD  + S I++DPYAK++  +              FLG+I     FDW G
Sbjct: 304  QCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFDWDG 363

Query: 1797 DVHPRLSMEKLMVYRLNVMDFTKDASSNLPNEIRGTFSGVTEKLHHFKDLGVNAILLEPI 1618
            +VHP L MEKL VYRLNV  FT D SS LP +I GTFSG+T+KL HFK+LGVNA+LLEPI
Sbjct: 364  EVHPNLPMEKLXVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLLEPI 423

Query: 1617 VPFDKQVGPYFPCHFFSPQNSFGPHGGSTSTIKSMKEMVKRLHANGIEILLEVVLTHTCD 1438
              FD++ GPYFP HFFSP N++GP G S S I SMKEMVK LHANG+E++LEVV THT  
Sbjct: 424  FQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTHTSG 483

Query: 1437 DAPLTKIDRPSYYLGKEGDDLATKNSLNCAHTVVQQLILDSLRHWVIEYHIDGFCFINAS 1258
            +  L  ID  SYY      +L  K++LNC + +VQQL+LDSLR+WV E+H+DGFCF+NAS
Sbjct: 484  NGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFVNAS 543

Query: 1257 SMLRGFNGENLSRPPLIEAIAFDPILSKTKIIADSFDPFHKSAKEIQFPHWKRWAEMNTK 1078
             +LRG +GE LSRPP +EAIAFDP+LSKTK++AD +DP    +KE +FPHWKRWAE+N+K
Sbjct: 544  FLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAEVNSK 603

Query: 1077 FCSDARNYLRGKTLLSNLATRLCGSGDIFLNGRGPAFSFNFVSRNHGLSLVDLVSFSS-- 904
            FCSD R++ RG+ L+S+LATRLCGSGD+F +GRGPAFSFNF++RN GL LVDLVSFS+  
Sbjct: 604  FCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFSNSN 663

Query: 903  -ATELSWNCGEEGATKKKGVLETRLKQIRNFLFILYVSLGVPVLNMGDECGRSSGGSLEH 727
             A+ELSWNCGEEG T    VLE RLKQIRNF+F+L+VSLGVPVLNMGDECG+SSGGS+  
Sbjct: 664  LASELSWNCGEEGPTSNLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGGSVAF 723

Query: 726  SDRKPFNWKALETGFAIQTTQFISFLSTLKIRRSDLLQSREFLKVENIDWHGTNLSPPDW 547
            +D++ FNW  L+T F  QTTQFI+FLS+ + RR DL Q+R FLK ENIDW   N SPP W
Sbjct: 724  NDKRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQNRNFLKGENIDWFDNNQSPPQW 783

Query: 546  EDPTSKFLAVSL--XXXXXXXXDEN-QLNGDMFVAFNGGDDPMIATLPPPASNMVWLRLV 376
            ED + KFLAV L           EN +   ++F+ FN  D      LP P     W R+V
Sbjct: 784  EDASCKFLAVMLRADKEENESITENPKTRSNIFMVFNASDQSESVALPEPLEGTSWFRVV 843

Query: 375  DTALRYPGFFSTAGDPTPEKIPGSPVYEMAPHSCVLLEARSVD 247
            DTAL +PGFFS+ G+  P  + GS  YE+  HSC L EA+S +
Sbjct: 844  DTALPFPGFFSSDGELVP--MTGSVTYEIQAHSCALFEAKSAN 884