BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes22_contig00011844
         (1722 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ABC94481.1| putative steroid 23-alpha-hydroxylase cytochrome ...   793   0.0  
dbj|BAE16977.1| steroid 23-alpha-hydroxylase [Zinnia elegans]         754   0.0  
dbj|BAE16978.1| steroid 23-alpha-hydroxylase [Zinnia elegans]         733   0.0  
gb|AAZ39038.1| cytochrome P450 90A2 [Camellia japonica]               697   0.0  
emb|CAD27417.1| cytochrome P450 [Nicotiana tabacum]                   677   0.0  

>gb|ABC94481.1| putative steroid 23-alpha-hydroxylase cytochrome P450 [Artemisia
            annua]
          Length = 473

 Score =  793 bits (2047), Expect = 0.0
 Identities = 393/457 (85%), Positives = 420/457 (91%)
 Frame = +3

Query: 255  IIFLVFRITTASRRKISMPPGSIGLPLIGETFQLISAYKTENPEPFIDTRVSKYGTVFTT 434
            IIFL+FR  +A  R I+ PPGS GLPLIGETFQ+ISAYKTENPEPFID RV KYG+VFTT
Sbjct: 17   IIFLLFRRNSAFWRSINTPPGSTGLPLIGETFQIISAYKTENPEPFIDARVXKYGSVFTT 76

Query: 435  HVFGERTVFSADAETNRFILQNEGRLFESSYPGSISNLVGKHSLLLMRGSLHRRMHSLTM 614
            HVFGERTVFSAD E N+FILQNEGRLFESSYPGSI+NLVGKHSLLLMRG LHRRMHSLTM
Sbjct: 77   HVFGERTVFSADVEINKFILQNEGRLFESSYPGSIANLVGKHSLLLMRGGLHRRMHSLTM 136

Query: 615  SFANSTIIKDHLLVDIDRLVRLNLDSWIGRILLMDEAKKITFELTLKQLLSIDPCEWTEN 794
            SFANSTIIKDHLL DIDRLVRLNLDSW GRILLMDEAKKITFELTLKQLLSI+PCEW+EN
Sbjct: 137  SFANSTIIKDHLLADIDRLVRLNLDSWTGRILLMDEAKKITFELTLKQLLSIEPCEWSEN 196

Query: 795  LRKEYMLVIEGFFCIPLPFFSLTYLRAVQARKRVTEALNLVVXXXXXXXXXGVKKNDMLA 974
            LRKEYMLVIEGFFCIPLPFFSLTY RA+QARKRVTEAL+LVV         GVKKNDMLA
Sbjct: 197  LRKEYMLVIEGFFCIPLPFFSLTYRRAIQARKRVTEALHLVVRERRIEREKGVKKNDMLA 256

Query: 975  ALFDSDGEGVIFRDDEIVDFLVSLLVAGYDTTSTTMTLAVKFLTDTPLALAELKEEHDEI 1154
            ALFDSDG+   F DDEIVDFLVSLLVAGYDTTSTTMTLAVK+LTDTP ALA+LKEEHD I
Sbjct: 257  ALFDSDGDEENFYDDEIVDFLVSLLVAGYDTTSTTMTLAVKYLTDTPSALAQLKEEHDAI 316

Query: 1155 RARKGTSVGLEWDDYKSMPFTQCVVNETLRLSNIISGVFRRAMTDVQIKGYTIPKGSKVF 1334
            RA+KG SV LEWDDYKSMPFTQCVVNETLRLSNIISGVFRRA+TDV+IK YTIPKG+KVF
Sbjct: 317  RAKKGASVALEWDDYKSMPFTQCVVNETLRLSNIISGVFRRAITDVKIKDYTIPKGAKVF 376

Query: 1335 TSLRAVHLGQDNFEDARIFDPWRWQKTSDPANFMPFGGGPRRCPGYELARVALSVFLHHL 1514
            TSLRAVHL QDNFEDAR+F+PWRWQKTSD A+FMPFGGGPRRCPG+ELARVALSVFLHHL
Sbjct: 377  TSLRAVHLDQDNFEDARVFNPWRWQKTSDSASFMPFGGGPRRCPGHELARVALSVFLHHL 436

Query: 1515 VTRFSWKPAEEDKLVFFPTTRTKKRYPIIVQHRNMVG 1625
            +T FSWKPAEEDK+VFFPTTRT+KRYPIIV+ RN+VG
Sbjct: 437  ITHFSWKPAEEDKVVFFPTTRTQKRYPIIVEQRNVVG 473


>dbj|BAE16977.1| steroid 23-alpha-hydroxylase [Zinnia elegans]
          Length = 471

 Score =  754 bits (1946), Expect = 0.0
 Identities = 377/455 (82%), Positives = 405/455 (89%)
 Frame = +3

Query: 258  IFLVFRITTASRRKISMPPGSIGLPLIGETFQLISAYKTENPEPFIDTRVSKYGTVFTTH 437
            I ++   TT+SRR    PPG+ G+P IGET QLISAYKTENPEPFIDTRV KYG VFTTH
Sbjct: 21   ILIILHTTTSSRRNHHSPPGATGIPFIGETLQLISAYKTENPEPFIDTRVEKYGVVFTTH 80

Query: 438  VFGERTVFSADAETNRFILQNEGRLFESSYPGSISNLVGKHSLLLMRGSLHRRMHSLTMS 617
            VFGERTVFSAD ETNRFIL+NEGRLFESSYPGSI+NLVGK+SLLLMRG LHRRMHSLTMS
Sbjct: 81   VFGERTVFSADGETNRFILKNEGRLFESSYPGSIANLVGKNSLLLMRGGLHRRMHSLTMS 140

Query: 618  FANSTIIKDHLLVDIDRLVRLNLDSWIGRILLMDEAKKITFELTLKQLLSIDPCEWTENL 797
            FANSTIIKDHLLVDIDRLVRLNLD+W GRILLMDEAKKITFELTLKQLLSI+P EW+ENL
Sbjct: 141  FANSTIIKDHLLVDIDRLVRLNLDAWTGRILLMDEAKKITFELTLKQLLSIEPFEWSENL 200

Query: 798  RKEYMLVIEGFFCIPLPFFSLTYLRAVQARKRVTEALNLVVXXXXXXXXXGVKKNDMLAA 977
            RKEYMLVIEGFFCIPLPFF LTY RA+QARKRVTEAL+LVV         GVKKNDMLAA
Sbjct: 201  RKEYMLVIEGFFCIPLPFFPLTYRRAIQARKRVTEALHLVVRERRVERERGVKKNDMLAA 260

Query: 978  LFDSDGEGVIFRDDEIVDFLVSLLVAGYDTTSTTMTLAVKFLTDTPLALAELKEEHDEIR 1157
            LFD+D +     DDEIVD LVSLLVAGYDTT TTMTLAVK+LTDTP ALA+LKEEH++I 
Sbjct: 261  LFDNDCD-----DDEIVDLLVSLLVAGYDTTPTTMTLAVKYLTDTPSALAQLKEEHEQIT 315

Query: 1158 ARKGTSVGLEWDDYKSMPFTQCVVNETLRLSNIISGVFRRAMTDVQIKGYTIPKGSKVFT 1337
             +KG  V LEWDDYKSMPFTQCVVNETLRLSNIISGVFRRAMTDV+IK +TIPKGSKVF 
Sbjct: 316  RKKGALVALEWDDYKSMPFTQCVVNETLRLSNIISGVFRRAMTDVKIKDFTIPKGSKVFA 375

Query: 1338 SLRAVHLGQDNFEDARIFDPWRWQKTSDPANFMPFGGGPRRCPGYELARVALSVFLHHLV 1517
            SLRAVHL  +NFEDAR+F PWRWQK SDPANFMPFGGGPRRCPG+ELARVALSVFLHHL+
Sbjct: 376  SLRAVHLDLENFEDARVFYPWRWQKISDPANFMPFGGGPRRCPGHELARVALSVFLHHLI 435

Query: 1518 TRFSWKPAEEDKLVFFPTTRTKKRYPIIVQHRNMV 1622
            TRFSW PAEEDKLVFFPTTRT+KRYPIIVQ RN+V
Sbjct: 436  TRFSWIPAEEDKLVFFPTTRTQKRYPIIVQKRNVV 470


>dbj|BAE16978.1| steroid 23-alpha-hydroxylase [Zinnia elegans]
          Length = 491

 Score =  733 bits (1891), Expect = 0.0
 Identities = 362/450 (80%), Positives = 400/450 (88%), Gaps = 2/450 (0%)
 Frame = +3

Query: 264  LVFRITTASRRKISMPPGSIGLPLIGETFQLISAYKTENPEPFIDTRVSKYGTVFTTHVF 443
            L+   TT+SRRK  +PPG++GLP IGE  QLI AYKT+NPEPFID+RV+KYGT+FTTHVF
Sbjct: 17   LLHLATTSSRRKTRLPPGTVGLPFIGEXLQLIXAYKTQNPEPFIDSRVAKYGTLFTTHVF 76

Query: 444  GERTVFSADAETNRFILQNEGRLFESSYPGSISNLVGKHSLLLMRGSLHRRMHSLTMSFA 623
            GERTVFSAD ETNRFILQNEGRLFESSYPGSI NLVGKHSL  MRGSLH+RMHSLTMSFA
Sbjct: 77   GERTVFSADPETNRFILQNEGRLFESSYPGSILNLVGKHSLSFMRGSLHKRMHSLTMSFA 136

Query: 624  NSTIIKDHLLVDIDRLVRLNLDSWIGRILLMDEAKKITFELTLKQLLSIDPCEWTENLRK 803
            NSTIIKDHLLVDIDRLVRLNLDSW GR+LL++EAKKITFELTLKQLLSI+PCEWTENLRK
Sbjct: 137  NSTIIKDHLLVDIDRLVRLNLDSWTGRVLLLEEAKKITFELTLKQLLSIEPCEWTENLRK 196

Query: 804  EYMLVIEGFFCIPLPFFSLTYLRAVQARKRVTEALNLVVXXXXXXXXXGVKKNDMLAALF 983
             YMLVIEGFFCIP+P FS+TY RA++AR+RV E L+LVV         G+KKNDMLAALF
Sbjct: 197  IYMLVIEGFFCIPIPMFSITYRRAIKARERVAEELSLVVRERRRESEKGMKKNDMLAALF 256

Query: 984  DSDGE--GVIFRDDEIVDFLVSLLVAGYDTTSTTMTLAVKFLTDTPLALAELKEEHDEIR 1157
            D  G+  GV   D EIVDFLVSLLVAGYDTTSTTMTLAVKFLTDTP ALA+LKEE DEIR
Sbjct: 257  DGGGDVGGVGLTDAEIVDFLVSLLVAGYDTTSTTMTLAVKFLTDTPRALAQLKEEQDEIR 316

Query: 1158 ARKGTSVGLEWDDYKSMPFTQCVVNETLRLSNIISGVFRRAMTDVQIKGYTIPKGSKVFT 1337
            ARK  +  +EW+DYKSMPFTQCV+NETLR+SNIISGVFRRAMTDV IKGY IP+GSKVFT
Sbjct: 317  ARKTDAGTIEWEDYKSMPFTQCVINETLRVSNIISGVFRRAMTDVDIKGYRIPRGSKVFT 376

Query: 1338 SLRAVHLGQDNFEDARIFDPWRWQKTSDPANFMPFGGGPRRCPGYELARVALSVFLHHLV 1517
            SLRAVHLG +NF DAR+FD WRWQK+S+P NFMPFGGGPR+CPG ELARVALSVFLHHLV
Sbjct: 377  SLRAVHLGHENFNDARVFDLWRWQKSSNPTNFMPFGGGPRKCPGNELARVALSVFLHHLV 436

Query: 1518 TRFSWKPAEEDKLVFFPTTRTKKRYPIIVQ 1607
            TRFSW+PAEEDKL+FFPTTRT+KRYPII +
Sbjct: 437  TRFSWEPAEEDKLMFFPTTRTQKRYPIIXE 466


>gb|AAZ39038.1| cytochrome P450 90A2 [Camellia japonica]
          Length = 484

 Score =  697 bits (1799), Expect = 0.0
 Identities = 344/464 (74%), Positives = 396/464 (85%), Gaps = 5/464 (1%)
 Frame = +3

Query: 255  IIFLVFRITTASRRKISMPPGSIGLPLIGETFQLISAYKTENPEPFIDTRVSKYGTVFTT 434
            +IF++ R +  +RR + +PPG++GLP +GET  LISAYK+ENP+PFID RVS++G +FTT
Sbjct: 19   LIFVLLRSSIITRRHLRLPPGTLGLPFLGETLHLISAYKSENPDPFIDDRVSRFGPIFTT 78

Query: 435  HVFGERTVFSADAETNRFILQNEGRLFESSYPGSISNLVGKHSLLLMRGSLHRRMHSLTM 614
            HVFGE TVFSAD ETNRFILQNEG+LFESSYPGSISNL+G+HSLLLM+GSLH+RMHSLTM
Sbjct: 79   HVFGEPTVFSADPETNRFILQNEGKLFESSYPGSISNLLGRHSLLLMKGSLHKRMHSLTM 138

Query: 615  SFANSTIIKDHLLVDIDRLVRLNLDSWIGRILLMDEAKKITFELTLKQLLSIDPCEWTEN 794
            SFANS+II+DHL++DIDRLVRLNL+SW GR+LLM+EAKKITFELT+KQL+S DPCEWTE+
Sbjct: 139  SFANSSIIRDHLMLDIDRLVRLNLESWTGRVLLMEEAKKITFELTVKQLMSFDPCEWTES 198

Query: 795  LRKEYMLVIEGFFCIPLPFFSLTYLRAVQARKRVTEALNLVV-XXXXXXXXXGVKKNDML 971
            LRKEYMLVIEGFF IPLP FS TY RA+QAR +V EAL+LVV          G +KNDML
Sbjct: 199  LRKEYMLVIEGFFTIPLPLFSSTYRRAIQARSKVAEALSLVVRERRRESSERGERKNDML 258

Query: 972  AALFDSDGEGVIFRDDEIVDFLVSLLVAGYDTTSTTMTLAVKFLTDTPLALAELKEEHDE 1151
             AL D +G G  F D+EIVDFLV+LLVAGY+TTST MTLAVKFLT+TPLALA+LKEEHDE
Sbjct: 259  GALMDEEGGGGGFSDEEIVDFLVALLVAGYETTSTIMTLAVKFLTETPLALAQLKEEHDE 318

Query: 1152 IRARKGTSVGLEWDDYKSMPFTQCVVNETLRLSNIISGVFRRAMTDVQIKGYTIPKGSKV 1331
            IRARK     LEW+DYKSMPFTQCVVNETLR++NIISGVFRRA+TDV +KGYTIPKG KV
Sbjct: 319  IRARKDNLEALEWNDYKSMPFTQCVVNETLRVANIISGVFRRAITDVDVKGYTIPKGWKV 378

Query: 1332 FTSLRAVHLGQDNFEDARIFDPWRWQKTSDPAN----FMPFGGGPRRCPGYELARVALSV 1499
            F SLRAVH+  D+F+DAR F+PWRWQ  S   N    F PFGGGPRRCPG ELARV LSV
Sbjct: 379  FASLRAVHMDHDHFKDARSFNPWRWQTNSGTTNSVNLFTPFGGGPRRCPGAELARVELSV 438

Query: 1500 FLHHLVTRFSWKPAEEDKLVFFPTTRTKKRYPIIVQHRNMVG*C 1631
            FLHHLVTRFSW PAEEDKLVFFPTTRT+KRYPII+Q RN+   C
Sbjct: 439  FLHHLVTRFSWVPAEEDKLVFFPTTRTQKRYPIILQRRNVPTSC 482


>emb|CAD27417.1| cytochrome P450 [Nicotiana tabacum]
          Length = 483

 Score =  677 bits (1748), Expect = 0.0
 Identities = 343/468 (73%), Positives = 383/468 (81%), Gaps = 9/468 (1%)
 Frame = +3

Query: 255  IIFLVFRITTAS---RRKISMPPGSIGLPLIGETFQLISAYKTENPEPFIDTRVSKYGTV 425
            I FL+ R   A+   RRK  +PPG++GLP IGET QLISAYKTENPEPFID RVSKYG +
Sbjct: 16   ITFLLLRAAAAAHFRRRKTRLPPGTLGLPFIGETLQLISAYKTENPEPFIDDRVSKYGNI 75

Query: 426  FTTHVFGERTVFSADAETNRFILQNEGRLFESSYPGSISNLVGKHSLLLMRGSLHRRMHS 605
            FTTH+FGE TVFS DAETNRFILQNEGR FESSYP S+ NL+GKHSLLLMRGSLH+RMHS
Sbjct: 76   FTTHIFGEPTVFSTDAETNRFILQNEGRPFESSYPSSLQNLLGKHSLLLMRGSLHKRMHS 135

Query: 606  LTMSFANSTIIKDHLLVDIDRLVRLNLDSWIGRILLMDEAKKITFELTLKQLLSIDPCEW 785
            LTMSFANS+I+KDHLL DIDRLVRLNLDSW GR+ LM+EAKKITF LT+KQL+S+DPCEW
Sbjct: 136  LTMSFANSSILKDHLLADIDRLVRLNLDSWTGRVFLMEEAKKITFNLTVKQLMSLDPCEW 195

Query: 786  TENLRKEYMLVIEGFFCIPLPFFSLTYLRAVQARKRVTEALNLVVXXXXXXXXXGVK-KN 962
            TE L KEYMLVIEGFF IPLPFFS TY +A+QAR++V EAL LVV         G + KN
Sbjct: 196  TEKLMKEYMLVIEGFFTIPLPFFSSTYRKAIQARRKVAEALGLVVKERRKERGGGERLKN 255

Query: 963  DMLAALFDSDG-EGVIFRDDEIVDFLVSLLVAGYDTTSTTMTLAVKFLTDTPLALAELKE 1139
            DML ALF+ DG EG  F D+ IVDF+++LLVAGY+TTST MTLAVKFLT+TP AL+ LKE
Sbjct: 256  DMLEALFEGDGVEG--FSDEVIVDFMLALLVAGYETTSTIMTLAVKFLTETPHALSLLKE 313

Query: 1140 EHDEIRARKGTSVGLEWDDYKSMPFTQCVVNETLRLSNIISGVFRRAMTDVQIKGYTIPK 1319
            EH+EIR RKG    L W+DYKSMPFTQCVVNETLR+ NIISGVFRR MTD+ IKGYTIPK
Sbjct: 314  EHEEIRLRKGDVESLLWEDYKSMPFTQCVVNETLRVGNIISGVFRRTMTDINIKGYTIPK 373

Query: 1320 GSKVFTSLRAVHLGQDNFEDARIFDPWRWQ----KTSDPANFMPFGGGPRRCPGYELARV 1487
            G KVF   RAVHL  ++F+DAR FDPWRWQ     TS P  F PFGGGPRRCPGYELARV
Sbjct: 374  GWKVFACFRAVHLDHEHFKDARTFDPWRWQSNAGSTSSPNVFTPFGGGPRRCPGYELARV 433

Query: 1488 ALSVFLHHLVTRFSWKPAEEDKLVFFPTTRTKKRYPIIVQHRNMVG*C 1631
             LSVFLHHLVTR SW PAE DKLVFFPTTR +KRYPIIVQ R++   C
Sbjct: 434  ELSVFLHHLVTRHSWVPAEPDKLVFFPTTRMQKRYPIIVQRRSLFDPC 481