BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes22_contig00010774
         (1814 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002278289.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   581   e-163
ref|XP_003521174.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   543   e-152
ref|XP_002308030.1| predicted protein [Populus trichocarpa] gi|2...   527   e-147
emb|CAN82223.1| hypothetical protein VITISV_011871 [Vitis vinifera]   501   e-139
ref|NP_567993.1| cytoplasmic tRNA 2-thiolation protein 2 [Arabid...   501   e-139

>ref|XP_002278289.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Vitis vinifera]
            gi|297739656|emb|CBI29838.3| unnamed protein product
            [Vitis vinifera]
          Length = 468

 Score =  581 bits (1497), Expect = e-163
 Identities = 304/474 (64%), Positives = 357/474 (75%), Gaps = 13/474 (2%)
 Frame = -1

Query: 1754 MACNGTGCQSGCYKDSTGDEEEKISTEKKLIGDDSSDGNH---RRHQMICLKCKGTEATI 1584
            MAC    CQSGC++D   D++  ++ +KKL     S G+      H  +CLKCK  E   
Sbjct: 1    MACGAGNCQSGCFRD---DDDSVVTEKKKLTNRTLSSGSDIDLPNHHNLCLKCKVNETLA 57

Query: 1583 FTGG---DGG-GRFCADCFRSNLYGKFKLAVTSNAMITPTDKVLVAFSGGPSSRVALQFV 1416
             +G    DG  GRFC DCFR NL+GKFKLAVTSNAMI+PTD VLVAFSGGPSSRVALQFV
Sbjct: 58   SSGTVSVDGDIGRFCFDCFRGNLFGKFKLAVTSNAMISPTDNVLVAFSGGPSSRVALQFV 117

Query: 1415 SEMQLKAQKNFDATASRDRSLPVFGVGVAFIDESEIRPFSSGDLDKAVEDMKKIVLNLAP 1236
             EMQ KAQKNFDA  SRDRSLPVFGVGVAF+DES +    S + DKA++DMK IV NLAP
Sbjct: 118  HEMQHKAQKNFDA--SRDRSLPVFGVGVAFVDESAVSRIPSDEFDKAIQDMKSIVSNLAP 175

Query: 1235 PRKELHVAPIRSIYSTKTSDGSVTLKNLVNAVGDATGKEDLLSHLRMLSLQKIAIENGYT 1056
            P K  HV PI S+YS   S+G   L+ L+NAV D TGKEDLL HLRMLSLQK A ENGYT
Sbjct: 176  PDKAFHVVPIESVYS---SNGRDRLEELLNAVKDVTGKEDLLQHLRMLSLQKNAYENGYT 232

Query: 1055 KLVLGSCTSKIACHVLAATVKGQGYSLAADIQYADARWEIPVVLPLRDCLMQELNILCSL 876
            KL+LGSCTS+IACHV++ATVKGQGYSL ADIQY DARWEIPVVLPLRDCL QELN+LC L
Sbjct: 233  KLLLGSCTSRIACHVISATVKGQGYSLPADIQYVDARWEIPVVLPLRDCLAQELNMLCRL 292

Query: 875  DSLKIVEVFNDSCASINGLVSSFVKLLQEENPSRECTIARTAGKLTPFPFNRIPEANVDD 696
            D LK +EV N   + INGLV+SFV LLQEENPSRECTI RTAGKLTPF FN+IPE++  +
Sbjct: 293  DGLKTMEVLNSPHSGINGLVASFVTLLQEENPSRECTIVRTAGKLTPFHFNQIPESDDSN 352

Query: 695  VHLASQRRQKKFNLRPIESLPPESFCPICNSPMSDLQNLSCS---NPQPTPELFRAACCS 525
            VHLAS+RRQKKFNL+P ES+PPES+CPICNSP+S   +++ S   + Q   ++F A CC 
Sbjct: 353  VHLASRRRQKKFNLKPNESVPPESYCPICNSPLSKSDHINLSSLGSDQTNADIFGATCCP 412

Query: 524  CCQYQILPEEPSSMDEFYSFLPESITARVKDGN---QKWMREKIQDCLLLDGED 372
             CQ+QILP+EP SM+ FYS LP+    + KD +   Q+ +RE+IQD LL D ED
Sbjct: 413  SCQFQILPKEPVSMEHFYSLLPQQFVVQAKDRSFSMQRQLREQIQDFLLSDSED 466


>ref|XP_003521174.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2-like [Glycine max]
          Length = 458

 Score =  543 bits (1399), Expect = e-152
 Identities = 276/468 (58%), Positives = 351/468 (75%), Gaps = 6/468 (1%)
 Frame = -1

Query: 1754 MACNGTGCQSG-CYKDSTGDEEEKISTEKKLIGDDSSDGNHRRHQMICLKCKGTEATIFT 1578
            MACNG+GCQSG CYKD     ++   +E+    D  +  N      +C+KCK  +A    
Sbjct: 1    MACNGSGCQSGGCYKDEDATCKQPPKSEE---ADSKNPSN------VCIKCKLNDAVSGY 51

Query: 1577 GGDGGGRFCADCFRSNLYGKFKLAVTSNAMITPTDKVLVAFSGGPSSRVALQFVSEMQLK 1398
            GG   GRFCADCF++NL+GKF+ AVTSNAMITPTDKVLVAFSGGPSSRVALQFV +MQ +
Sbjct: 52   GGIDDGRFCADCFKTNLFGKFRFAVTSNAMITPTDKVLVAFSGGPSSRVALQFVHDMQER 111

Query: 1397 AQKNFDATASRDRSLPVFGVGVAFIDESEIRPFSSGDLDKAVEDMKKIVLNLAPPRKELH 1218
            AQ+NFDA  SRDRSLPVFGVGV FIDES + P  S ++++AVE + ++V +LAPPRKELH
Sbjct: 112  AQRNFDA--SRDRSLPVFGVGVVFIDESAVLPIPSNEMEEAVEVVSEVVSSLAPPRKELH 169

Query: 1217 VAPIRSIYSTKTSDGSVTLKNLVNAVGDATGKEDLLSHLRMLSLQKIAIENGYTKLVLGS 1038
            + PI ++YS+ + DG   +  +VN V D TG+ED+L  LRML+LQK+A E GY +++LGS
Sbjct: 170  IVPIETVYSSDSGDGKERITKVVNTVIDPTGREDVLLCLRMLALQKVASEFGYNRIILGS 229

Query: 1037 CTSKIACHVLAATVKGQGYSLAADIQYADARWEIPVVLPLRDCLMQELNILCSLDSLKIV 858
            C S+IACHV++ATVKGQGYSL ADIQY DARWE+PVVLPLRDC  QE+N+LC LD LK V
Sbjct: 230  CISRIACHVISATVKGQGYSLPADIQYVDARWEVPVVLPLRDCFAQEINMLCHLDGLKTV 289

Query: 857  EVFNDSCASINGLVSSFVKLLQEENPSRECTIARTAGKLTPFPFNRIPEANVDDVHLASQ 678
            ++  D C+SINGLVSSFV LLQEENPSRE TI RTAGKLTPF FNRIPE    +V LA++
Sbjct: 290  KLSTDLCSSINGLVSSFVALLQEENPSRESTIVRTAGKLTPFQFNRIPEIIDGNVPLATR 349

Query: 677  RRQKKFNLRPIESLPPESFCPICNSPM--SDLQNLSCSNPQPTPELFRAACCSCCQYQIL 504
            RRQK++NL+  ES+  ESFCP+CNSP+  S++ + S  +   + ++F   CCS C++QIL
Sbjct: 350  RRQKRYNLKSNESVSSESFCPLCNSPLDKSEITDWSNIDSHRSSDIFYNTCCSSCRFQIL 409

Query: 503  PEEPSSMDEFYSFLPESITARVK---DGNQKWMREKIQDCLLLDGEDE 369
            P +  +M++FY  LP+S+  R K   +GN   +RE+IQD LL DGEDE
Sbjct: 410  PSDSMAMEQFYMDLPQSVVGRAKQANNGNLSLLREQIQDYLLSDGEDE 457


>ref|XP_002308030.1| predicted protein [Populus trichocarpa] gi|222854006|gb|EEE91553.1|
            predicted protein [Populus trichocarpa]
          Length = 443

 Score =  527 bits (1357), Expect = e-147
 Identities = 269/446 (60%), Positives = 335/446 (75%), Gaps = 3/446 (0%)
 Frame = -1

Query: 1754 MACNGTGCQSGCYKDSTGDEEEKISTEKKLIGDDSSDGNHRRHQMICLKCKGTEATIFTG 1575
            M+CN +GCQS CYK+  GD+          +   ++  N      +CLKCK T + I  G
Sbjct: 1    MSCNSSGCQSNCYKN--GDQVSDSPPPPPTLAAANNSNN------LCLKCK-TNSPITPG 51

Query: 1574 GDGG--GRFCADCFRSNLYGKFKLAVTSNAMITPTDKVLVAFSGGPSSRVALQFVSEMQL 1401
               G   RFCADCFRSN+YGKF+LAVTS AMI+P+D VLVAFSGGPSSRVALQFV  +Q 
Sbjct: 52   SLSGDEARFCADCFRSNIYGKFRLAVTSQAMISPSDNVLVAFSGGPSSRVALQFVHGLQD 111

Query: 1400 KAQKNFDATASRDRSLPVFGVGVAFIDESEIRPFSSGDLDKAVEDMKKIVLNLAPPRKEL 1221
            +AQKNF+A  S+D+SLPVFGVGVAFIDES + P SS  +D A++DM+ +V NLAPP K+L
Sbjct: 112  RAQKNFEA--SKDKSLPVFGVGVAFIDESAVYPVSSDKVDGAIQDMRSVVSNLAPPTKQL 169

Query: 1220 HVAPIRSIYSTKTSDGSVTLKNLVNAVGDATGKEDLLSHLRMLSLQKIAIENGYTKLVLG 1041
            HV PI +IY++ +SDG   L  L++AV DATGKEDLL HLRML+LQ +A +NGY +L+LG
Sbjct: 170  HVVPIENIYASDSSDGKDRLTKLLDAVNDATGKEDLLLHLRMLALQMVASQNGYNRLILG 229

Query: 1040 SCTSKIACHVLAATVKGQGYSLAADIQYADARWEIPVVLPLRDCLMQELNILCSLDSLKI 861
            SCTS+IACHVLAATVKGQGYSL+ADIQY DARWEIPVVLPLRDC  QELN+LC LD LK 
Sbjct: 230  SCTSRIACHVLAATVKGQGYSLSADIQYVDARWEIPVVLPLRDCTSQELNLLCQLDGLKT 289

Query: 860  VEVFNDSCASINGLVSSFVKLLQEENPSRECTIARTAGKLTPFPFNRIPEANVDDVHLAS 681
            ++ F+ + + INGL+SSFV +LQEEN SRECTI RTAGKLTPF FNRIPE +  +V LA 
Sbjct: 290  LQPFDSTPSGINGLISSFVNVLQEENSSRECTILRTAGKLTPFEFNRIPETDNCNVPLAI 349

Query: 680  QRRQKKFNLRPIESLPPESFCPICNSPMSDLQNLSCSNPQP-TPELFRAACCSCCQYQIL 504
            +RRQK++NL+  ES+  E FCPICN+P+ +  +L+ S+ +      F AACCS CQ+QI+
Sbjct: 350  RRRQKRYNLKTKESISSELFCPICNAPLKNFNSLNLSSLKSCQSSRFNAACCSSCQFQII 409

Query: 503  PEEPSSMDEFYSFLPESITARVKDGN 426
            P++PSSM  F S LP+ +  R K GN
Sbjct: 410  PKDPSSMGNFCSLLPQQVVDRAKHGN 435


>emb|CAN82223.1| hypothetical protein VITISV_011871 [Vitis vinifera]
          Length = 747

 Score =  501 bits (1291), Expect = e-139
 Identities = 273/422 (64%), Positives = 311/422 (73%), Gaps = 15/422 (3%)
 Frame = -1

Query: 1754 MACNGTGCQSGCYKDSTGDEEEKISTEKKLIGDDSSDGNH---RRHQMICLKCKGTEATI 1584
            MAC    CQSGC++D   D++  ++ +KKL     S G+      H  +CLKCK  E   
Sbjct: 1    MACGAGNCQSGCFRD---DDDSVVTEKKKLTNRTLSSGSDIDLPNHHNLCLKCKVNETLA 57

Query: 1583 FTGG---DGG-GRFCADCFRSNLYGKFKLAVTSNAMITPTDKVLVAFSGGPSSRVALQFV 1416
             +G    DG  GRFC DCFR NL+GKFKLAVTSNAMI+PTD VLVAFSGGPSSRVALQFV
Sbjct: 58   SSGTVSVDGDIGRFCFDCFRGNLFGKFKLAVTSNAMISPTDNVLVAFSGGPSSRVALQFV 117

Query: 1415 SEMQLKAQKNFDATASRDRSLPVFGVGVAFIDESEIRPFSSGDLDKAVEDMKKIVLNLAP 1236
             EMQ KAQKNFDA  SRDRSLPVFGVGVAF+DES +    S + DKA++DMK IV NLAP
Sbjct: 118  HEMQHKAQKNFDA--SRDRSLPVFGVGVAFVDESAVSRIPSDEFDKAIQDMKSIVSNLAP 175

Query: 1235 PRKELHVAPIRSIYSTKTSDGSVTLKNLVNAVGDATGKEDLLSHLRMLSLQKIAIENGYT 1056
            P K  HV PI S+YS   S+G   L+ L+NAV D TGKEDLL HLRMLSLQK A ENGYT
Sbjct: 176  PXKAFHVVPIESVYS---SNGRDRLEELLNAVKDVTGKEDLLQHLRMLSLQKNAYENGYT 232

Query: 1055 KLVLGSCTSKIACHVLAATVKGQGYSLAADIQYADARWEIPVVLPLRDCLMQELNILCSL 876
            KL+LGSCTS+IACHV++ATVKGQGYSL ADIQY DARWEIPVVLPLRDCL QELN+LC L
Sbjct: 233  KLLLGSCTSRIACHVISATVKGQGYSLPADIQYVDARWEIPVVLPLRDCLAQELNMLCRL 292

Query: 875  D--------SLKIVEVFNDSCASINGLVSSFVKLLQEENPSRECTIARTAGKLTPFPFNR 720
            D        SLK +EV N   + INGLV+SFV LLQEENPSRECTI RTAGKLTPF FN+
Sbjct: 293  DGFVIHSFGSLKTMEVLNSPHSGINGLVASFVTLLQEENPSRECTIVRTAGKLTPFHFNQ 352

Query: 719  IPEANVDDVHLASQRRQKKFNLRPIESLPPESFCPICNSPMSDLQNLSCSNPQPTPELFR 540
            IPE++  +VHLAS+RRQKKFNL+P ES+PPES+C    S        +CSNP    EL  
Sbjct: 353  IPESDDSNVHLASRRRQKKFNLKPNESVPPESYC----SLSCFFYPYTCSNPGAGNELSE 408

Query: 539  AA 534
            AA
Sbjct: 409  AA 410


>ref|NP_567993.1| cytoplasmic tRNA 2-thiolation protein 2 [Arabidopsis thaliana]
            gi|332278212|sp|O65628.3|CTU2_ARATH RecName:
            Full=Cytoplasmic tRNA 2-thiolation protein 2
            gi|115646788|gb|ABJ17118.1| At4g35910 [Arabidopsis
            thaliana] gi|332661188|gb|AEE86588.1| cytoplasmic tRNA
            2-thiolation protein 2 [Arabidopsis thaliana]
          Length = 458

 Score =  501 bits (1290), Expect = e-139
 Identities = 265/471 (56%), Positives = 342/471 (72%), Gaps = 8/471 (1%)
 Frame = -1

Query: 1754 MACNGTGCQSGCYKDSTGDEEEKISTEKKLIGDDSSDGNHRRHQMICLKCKGTEATIFTG 1575
            MACN +GC+SGCY      + EK     K++ D  S G +  H+ +C+KCK      F  
Sbjct: 1    MACNSSGCESGCY------DREK-DNGSKIVDDAVSGGGN--HESVCVKCKCNAPMTF-- 49

Query: 1574 GDGG---GRFCADCFRSNLYGKFKLAVTSNAMITPTDKVLVAFSGGPSSRVALQFVSEMQ 1404
            GDGG   GRFCADCFR+N++GKF+LAVTS+AMITP+D VLVAFSGG SSRV+LQFV E+Q
Sbjct: 50   GDGGFDDGRFCADCFRNNVFGKFRLAVTSHAMITPSDNVLVAFSGGSSSRVSLQFVHELQ 109

Query: 1403 LKAQKNFDATASRDRSLPVFGVGVAFIDESEIRPFSSGDLDKAVEDMKKIVLNLAPPRKE 1224
            +KA KN++A  SRDRSLPVFGVGVAF+DE+   P  S ++  A+E ++  V  L+PP K+
Sbjct: 110  IKALKNYEA--SRDRSLPVFGVGVAFVDETAAFPALSTEMIDAIEWVRYTVSCLSPPAKD 167

Query: 1223 LHVAPIRSIYSTKTSDGSVTLKNLVNAVGDATGKEDLLSHLRMLSLQKIAIENGYTKLVL 1044
            LHV P+ SI+ + + D    L  L+++V D TGKEDLL HL+MLSLQK+A ENGY +LVL
Sbjct: 168  LHVVPVESIFGSDSLDARDRLLKLLDSVPDDTGKEDLLLHLKMLSLQKVAAENGYNRLVL 227

Query: 1043 GSCTSKIACHVLAATVKGQGYSLAADIQYADARWEIPVVLPLRDCLMQELNILCSLDSLK 864
            GSCTS+IA HVL ATVKG+GYSL+ADIQ+ DARW++P+VLPLRDC+  E+  LC LD LK
Sbjct: 228  GSCTSRIASHVLTATVKGRGYSLSADIQHVDARWKVPIVLPLRDCVRLEITRLCLLDGLK 287

Query: 863  IVEVFNDSCASINGLVSSFVKLLQEENPSRECTIARTAGKLTPFPFNRIPEANVDDVHLA 684
             VE+   S   IN LVSSFV LLQEENPSRECTI RTA KLTPF FN+IPE +  +V +A
Sbjct: 288  TVELACRSQCGINDLVSSFVALLQEENPSRECTIVRTAAKLTPFYFNKIPETDDSNVPMA 347

Query: 683  SQRRQKKFNLRPIESLPPESFCPICNSPM--SDLQNLSCSNPQPTPELFRAACCSCCQYQ 510
            +QRR K+FNL+   S+  E+FCPICN P+  SD   L         ++  AACCS C++Q
Sbjct: 348  TQRRLKRFNLKYDGSMTTEAFCPICNGPLNRSDSSELDTFEEGQESDVLYAACCSSCRFQ 407

Query: 509  ILPEEPSSMDEFYSFLPESITARVKD---GNQKWMREKIQDCLLLDGEDEV 366
            ILP++ SS+++F SFLP+ + ++VK     +Q ++REKI+DCLLLD E+ V
Sbjct: 408  ILPQDGSSLEQFSSFLPDHMISQVKHQKVDSQAYLREKIKDCLLLDDEEVV 458