BLASTX nr result
ID: Atractylodes22_contig00009985
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Atractylodes22_contig00009985
(1786 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|P24465.2|C71A1_PERAE RecName: Full=Cytochrome P450 71A1; AltN... 162 3e-93
gb|ADO16182.1| cytochrome P450 mono-oxygenase [Artemisia annua] 234 2e-91
gb|ADO16183.1| cytochrome P450 mono-oxygenase [Artemisia annua] 234 6e-90
emb|CBI14920.3| unnamed protein product [Vitis vinifera] 160 3e-86
ref|XP_003615865.1| Cytochrome P450 [Medicago truncatula] gi|355... 156 5e-85
>sp|P24465.2|C71A1_PERAE RecName: Full=Cytochrome P450 71A1; AltName: Full=ARP-2; AltName:
Full=CYPLXXIA1 gi|166949|gb|AAA32913.1| cytochrome
P-450LXXIA1 (cyp71A1) [Persea americana]
Length = 502
Score = 162 bits (409), Expect(4) = 3e-93
Identities = 80/165 (48%), Positives = 111/165 (67%)
Frame = -2
Query: 1740 PPGPPGLPFIGNLHQIDYSRLHTSLWHLSKSYGPIISLHFGFIPTIVVSSASLAKEVMKT 1561
PP PP LP IGNLHQ+ + H SL L+ GP+I LH G IPT++VS+A +A+E++KT
Sbjct: 32 PPSPPNLPIIGNLHQLG-NLPHRSLRSLANELGPLILLHLGHIPTLIVSTAEIAEEILKT 90
Query: 1560 QDLIFCSRPSLVGQLKVSYNGLDVIFSPYNEYWRDMRKIFMIHLLGPKRVQSFRHIREDE 1381
DLIF SRPS ++ Y+ DV FSPY EYWR +RKI ++ LL KRV S+R IRE+E
Sbjct: 91 HDLIFASRPSTTAARRIFYDCTDVAFSPYGEYWRQVRKICVLELLSIKRVNSYRSIREEE 150
Query: 1380 VSIAMKSIHRLALSSTHVNLSEIMKNVTGTIMIRVGFGKRYDEDE 1246
V + M+ I + + VNLSE++ ++ + RV FGK+Y+ +E
Sbjct: 151 VGLMMERISQSCSTGEAVNLSELLLLLSSGTITRVAFGKKYEGEE 195
Score = 117 bits (292), Expect(4) = 3e-93
Identities = 52/84 (61%), Positives = 66/84 (78%)
Frame = -2
Query: 465 LHGYKIKQKTLVFVNALAIGRDPECWESPEEFLPERFLGSDIEFKGNDFGLIPFGAGRRI 286
+ GY I KT VF+NA AIGRDP+ WE+ EEFLPERF+ + ++FKG DF LIPFGAGRR
Sbjct: 383 IRGYHIPAKTRVFINAWAIGRDPKSWENAEEFLPERFVNNSVDFKGQDFQLIPFGAGRRG 442
Query: 285 CPGISMGAVTVDLLLANLVYLFDW 214
CPGI+ G +V++ LANL+Y F+W
Sbjct: 443 CPGIAFGISSVEISLANLLYWFNW 466
Score = 104 bits (260), Expect(4) = 3e-93
Identities = 66/201 (32%), Positives = 103/201 (51%), Gaps = 4/201 (1%)
Frame = -3
Query: 1124 VSDLWPGMPFVGLVDRFLGKMDRLEKCFQFFDSFYQELIDEHLNPQNAKSHE--EEEDFI 951
V D +P +V D G RL++ D+F +ID+HL + A + E++D +
Sbjct: 217 VGDYFPSFAWV---DVLTGMDARLKRNHGELDAFVDHVIDDHLLSRKANGSDGVEQKDLV 273
Query: 950 DILLRLKKDQLFD--LTYDHIKAMLMDVLVAGTDTSSATVVWAMTALIKNPNVMKKAQEE 777
D+LL L+KD L +++KA+++D+ GTDT++ T+ WAM LIK+P+VM+KAQ+E
Sbjct: 274 DVLLHLQKDSSLGVHLNRNNLKAVILDMFSGGTDTTAVTLEWAMAELIKHPDVMEKAQQE 333
Query: 776 VXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLPKLTYLKAVVKEIMRL 597
V L +L YLK ++KE +RL
Sbjct: 334 V----------------------------RRVVGKKAKVEEEDLHQLHYLKLIIKETLRL 365
Query: 596 YPSVPLLVHRETTKDTILHGY 534
+P PLLV RE+T+D ++ GY
Sbjct: 366 HPVAPLLVPRESTRDVVIRGY 386
Score = 29.6 bits (65), Expect(4) = 3e-93
Identities = 13/37 (35%), Positives = 21/37 (56%)
Frame = -1
Query: 130 DWGLPDGMRMEDVDFEVLPGLTMHKRNELCLLAHAYL 20
+W LP + ED+D G+T+H + L L+A +L
Sbjct: 465 NWELPGDLTKEDLDMSEAVGITVHMKFPLQLVAKRHL 501
>gb|ADO16182.1| cytochrome P450 mono-oxygenase [Artemisia annua]
Length = 515
Score = 188 bits (478), Expect(3) = 2e-91
Identities = 107/201 (53%), Positives = 125/201 (62%), Gaps = 3/201 (1%)
Frame = -3
Query: 1124 VSDLWPGMPFVGLVDRFLGKMDRLEKCFQFFDSFYQELIDEHLNPQNAKSHEEEEDFIDI 945
+SDLWP P VGL+DR LGK RLEKC Q DSF +LIDEHL+ + +K +EE ED IDI
Sbjct: 226 ISDLWPDFPLVGLIDRLLGKFYRLEKCLQGLDSF-TKLIDEHLDAEYSKPNEEHEDLIDI 284
Query: 944 LLRLKKDQL---FDLTYDHIKAMLMDVLVAGTDTSSATVVWAMTALIKNPNVMKKAQEEV 774
LL+L+ QL F+LT DH+KAML D+LVAGTD S+AT+VWAMT L+K P MKKAQEEV
Sbjct: 285 LLQLRNGQLSDSFELTNDHMKAMLTDILVAGTDNSAATLVWAMTTLVKYPKAMKKAQEEV 344
Query: 773 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLPKLTYLKAVVKEIMRLY 594
LPKLTYLKAVVKE+MRLY
Sbjct: 345 ----------------------------RKMVQNKDKVDEDDLPKLTYLKAVVKEVMRLY 376
Query: 593 PSVPLLVHRETTKDTILHGYK 531
P+ PLL+ R TTKD IL YK
Sbjct: 377 PAAPLLIPRVTTKDAILLDYK 397
Score = 143 bits (360), Expect(3) = 2e-91
Identities = 66/82 (80%), Positives = 73/82 (89%)
Frame = -2
Query: 456 YKIKQKTLVFVNALAIGRDPECWESPEEFLPERFLGSDIEFKGNDFGLIPFGAGRRICPG 277
YKIKQ TLV+VNA+AIGRDPE WE+PEEF PERFLGSDI FKG+DF LIPFGAGRRICPG
Sbjct: 396 YKIKQNTLVYVNAMAIGRDPESWENPEEFSPERFLGSDIGFKGSDFELIPFGAGRRICPG 455
Query: 276 ISMGAVTVDLLLANLVYLFDWG 211
ISMG +V+L LANL+Y FDWG
Sbjct: 456 ISMGVNSVELFLANLIYSFDWG 477
Score = 54.7 bits (130), Expect(3) = 2e-91
Identities = 23/34 (67%), Positives = 27/34 (79%)
Frame = -1
Query: 130 DWGLPDGMRMEDVDFEVLPGLTMHKRNELCLLAH 29
DWGLPDG ++ED+D VLPGLTM + LCLLAH
Sbjct: 475 DWGLPDGTKIEDIDSGVLPGLTMTNKKGLCLLAH 508
Score = 234 bits (597), Expect = 6e-59
Identities = 112/174 (64%), Positives = 138/174 (79%)
Frame = -2
Query: 1773 SKTIINNSRFNPPGPPGLPFIGNLHQIDYSRLHTSLWHLSKSYGPIISLHFGFIPTIVVS 1594
SK+ ++ +PPGP G+PFIGNLHQID S H SLW LSKSYGP++SL+ GFIP IVVS
Sbjct: 30 SKSNVHGQFRSPPGPHGMPFIGNLHQIDKSNFHISLWSLSKSYGPVVSLNLGFIPAIVVS 89
Query: 1593 SASLAKEVMKTQDLIFCSRPSLVGQLKVSYNGLDVIFSPYNEYWRDMRKIFMIHLLGPKR 1414
SAS+AKE++KTQDL FCSRPS G +VSYNGLDV SPYN+ W++MR+IF ++L PKR
Sbjct: 90 SASVAKEILKTQDLTFCSRPSFHGLQRVSYNGLDVALSPYNKNWKEMRRIFTVYLFSPKR 149
Query: 1413 VQSFRHIREDEVSIAMKSIHRLALSSTHVNLSEIMKNVTGTIMIRVGFGKRYDE 1252
+QS R IREDEVS+AM+ IH LALSS HVNLSEI V ++ R+GFGKRY++
Sbjct: 150 LQSSRFIREDEVSLAMEKIHGLALSSKHVNLSEIAHIVMSNMVTRIGFGKRYED 203
>gb|ADO16183.1| cytochrome P450 mono-oxygenase [Artemisia annua]
Length = 491
Score = 181 bits (460), Expect(3) = 6e-90
Identities = 103/199 (51%), Positives = 120/199 (60%), Gaps = 3/199 (1%)
Frame = -3
Query: 1118 DLWPGMPFVGLVDRFLGKMDRLEKCFQFFDSFYQELIDEHLNPQNAKSHEEEEDFIDILL 939
D + P VGL+DR LGK RLEKC Q DSFYQ LIDEHL+ + +K +EE ED IDILL
Sbjct: 203 DGYESFPLVGLIDRLLGKFYRLEKCLQGLDSFYQNLIDEHLDAEYSKPNEEHEDLIDILL 262
Query: 938 RLKKDQL---FDLTYDHIKAMLMDVLVAGTDTSSATVVWAMTALIKNPNVMKKAQEEVXX 768
+L+ QL F+LT DH+KAML D+LVAGTD S+AT+VWAMT L+K P MKKAQEEV
Sbjct: 263 QLRNGQLSDSFELTNDHMKAMLTDILVAGTDNSAATLVWAMTTLVKYPKAMKKAQEEV-- 320
Query: 767 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLPKLTYLKAVVKEIMRLYPS 588
LPKLTYLKAVVKE+MRLYP+
Sbjct: 321 --------------------------RKMVQNKDKVDEDDLPKLTYLKAVVKEVMRLYPA 354
Query: 587 VPLLVHRETTKDTILHGYK 531
PLL+ R T KD L YK
Sbjct: 355 APLLIPRVTMKDATLLDYK 373
Score = 143 bits (360), Expect(3) = 6e-90
Identities = 66/82 (80%), Positives = 73/82 (89%)
Frame = -2
Query: 456 YKIKQKTLVFVNALAIGRDPECWESPEEFLPERFLGSDIEFKGNDFGLIPFGAGRRICPG 277
YKIKQ TLV+VNA+AIGRDPE WE+PEEF PERFLGSDI FKG+DF LIPFGAGRRICPG
Sbjct: 372 YKIKQNTLVYVNAMAIGRDPESWENPEEFSPERFLGSDIGFKGSDFELIPFGAGRRICPG 431
Query: 276 ISMGAVTVDLLLANLVYLFDWG 211
ISMG +V+L LANL+Y FDWG
Sbjct: 432 ISMGVNSVELFLANLIYSFDWG 453
Score = 56.2 bits (134), Expect(3) = 6e-90
Identities = 23/34 (67%), Positives = 28/34 (82%)
Frame = -1
Query: 130 DWGLPDGMRMEDVDFEVLPGLTMHKRNELCLLAH 29
DWGLPDG ++ED+D VLPGLTM + +LCLLAH
Sbjct: 451 DWGLPDGTKIEDIDSGVLPGLTMTNKKDLCLLAH 484
Score = 234 bits (597), Expect = 6e-59
Identities = 116/183 (63%), Positives = 139/183 (75%), Gaps = 7/183 (3%)
Frame = -2
Query: 1779 LLSKTIINNSRFN-------PPGPPGLPFIGNLHQIDYSRLHTSLWHLSKSYGPIISLHF 1621
L+ K I N S+ N PPGP G+PFIGNLHQID S H SLW LSKSYGP++SL+
Sbjct: 21 LIPKIIKNKSKSNVHGQFRSPPGPHGMPFIGNLHQIDKSNFHISLWSLSKSYGPVVSLNL 80
Query: 1620 GFIPTIVVSSASLAKEVMKTQDLIFCSRPSLVGQLKVSYNGLDVIFSPYNEYWRDMRKIF 1441
GFIP IVVSSAS+AKE++KTQDL FCSRPS G +VSYNGLDV SPYN+ W++MR+IF
Sbjct: 81 GFIPAIVVSSASVAKEILKTQDLTFCSRPSFHGLQRVSYNGLDVALSPYNKNWKEMRRIF 140
Query: 1440 MIHLLGPKRVQSFRHIREDEVSIAMKSIHRLALSSTHVNLSEIMKNVTGTIMIRVGFGKR 1261
++L PKR+QS R IREDEVS+AM+ IH LALSS HVNLSEI V + R+GFGKR
Sbjct: 141 TVYLFSPKRLQSSRFIREDEVSLAMEKIHGLALSSKHVNLSEIAHIVMSNMATRIGFGKR 200
Query: 1260 YDE 1252
Y++
Sbjct: 201 YED 203
>emb|CBI14920.3| unnamed protein product [Vitis vinifera]
Length = 527
Score = 160 bits (405), Expect(4) = 3e-86
Identities = 77/165 (46%), Positives = 112/165 (67%)
Frame = -2
Query: 1740 PPGPPGLPFIGNLHQIDYSRLHTSLWHLSKSYGPIISLHFGFIPTIVVSSASLAKEVMKT 1561
PP PP LP IGNLHQ+ H SLW L++ +GPI+ LHFG +P ++VS+A A+E+MKT
Sbjct: 46 PPSPPKLPIIGNLHQLGLLP-HRSLWALAQRHGPIMLLHFGKVPVVIVSAADAAREIMKT 104
Query: 1560 QDLIFCSRPSLVGQLKVSYNGLDVIFSPYNEYWRDMRKIFMIHLLGPKRVQSFRHIREDE 1381
D+IF +RP K+ Y+ DV +PY EYWR MR I ++HLL +RVQSFR +RE+E
Sbjct: 105 NDVIFLNRPKSSIFAKLLYDYKDVSMAPYGEYWRQMRSICVLHLLSNRRVQSFRGVREEE 164
Query: 1380 VSIAMKSIHRLALSSTHVNLSEIMKNVTGTIMIRVGFGKRYDEDE 1246
++ M+ I + SST ++LS++ ++T ++ RV G++Y DE
Sbjct: 165 TALLMEKISSSSSSSTPIDLSKMFLSLTNDLICRVALGRKYSGDE 209
Score = 103 bits (257), Expect(4) = 3e-86
Identities = 49/82 (59%), Positives = 57/82 (69%)
Frame = -2
Query: 459 GYKIKQKTLVFVNALAIGRDPECWESPEEFLPERFLGSDIEFKGNDFGLIPFGAGRRICP 280
GY I+ T V NA AIGRDP W+ EEF PERFL S I+F G DF LIPFGAGRR CP
Sbjct: 396 GYDIEVGTQVITNAWAIGRDPLLWDEAEEFRPERFLNSSIDFTGKDFELIPFGAGRRGCP 455
Query: 279 GISMGAVTVDLLLANLVYLFDW 214
G A+ +++ LANLV+ FDW
Sbjct: 456 GTLFAAMAIEVALANLVHQFDW 477
Score = 97.8 bits (242), Expect(4) = 3e-86
Identities = 62/199 (31%), Positives = 101/199 (50%), Gaps = 2/199 (1%)
Frame = -3
Query: 1124 VSDLWPGMPFVGLVDRFLGKMDRLEKCFQFFDSFYQELIDEHLNPQNAKSHEEEEDFIDI 945
V+D P + +V ++ G ++EK + FD F E++ EH+ + EE +DF+D+
Sbjct: 230 VADYIPWLSWVNFIN---GLDAKVEKVAKEFDRFLDEVVKEHVERRKRGVDEEVKDFVDV 286
Query: 944 LLRLKKDQLFD--LTYDHIKAMLMDVLVAGTDTSSATVVWAMTALIKNPNVMKKAQEEVX 771
LL +++D + +T IKA+ +D+ AG+DT+ + WAMT L+++P VM++ Q EV
Sbjct: 287 LLGIQEDNVTGVAITGVCIKALTLDMFAAGSDTTYTVLEWAMTELLRHPQVMRQLQNEV- 345
Query: 770 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLPKLTYLKAVVKEIMRLYP 591
L K+ YLKAV+KE +RL+P
Sbjct: 346 ---------------------------RGIAQGKLLITEDDLDKMQYLKAVIKETLRLHP 378
Query: 590 SVPLLVHRETTKDTILHGY 534
VPLL+ RE+T+ + GY
Sbjct: 379 PVPLLLPRESTRGAKIMGY 397
Score = 28.1 bits (61), Expect(4) = 3e-86
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = -1
Query: 130 DWGLPDGMRMEDVDFEVLPGLTMHKR 53
DW + G R ED+D GLT+H++
Sbjct: 476 DWEVGGGGRREDLDMTECTGLTIHRK 501
>ref|XP_003615865.1| Cytochrome P450 [Medicago truncatula] gi|355517200|gb|AES98823.1|
Cytochrome P450 [Medicago truncatula]
Length = 502
Score = 156 bits (395), Expect(4) = 5e-85
Identities = 87/180 (48%), Positives = 116/180 (64%), Gaps = 2/180 (1%)
Frame = -2
Query: 1779 LLSKTIINNSRFN-PPGPPGLPFIGNLHQIDYSRL-HTSLWHLSKSYGPIISLHFGFIPT 1606
++ K NNS N PPGP LP IGN+HQ+ + L H +L+ +YGP++ L G +
Sbjct: 25 IVKKWSCNNSSINLPPGPWTLPIIGNMHQLISNSLPHQCFKNLADTYGPLMHLKLGEVSY 84
Query: 1605 IVVSSASLAKEVMKTQDLIFCSRPSLVGQLKVSYNGLDVIFSPYNEYWRDMRKIFMIHLL 1426
++VSS S+AKE+MKT DL FC RP+L+ SYN +D+IFSPY E+WR +RKI ++ LL
Sbjct: 85 LIVSSPSMAKEIMKTHDLNFCDRPNLLLSTIFSYNAIDIIFSPYGEHWRQLRKICVLQLL 144
Query: 1425 GPKRVQSFRHIREDEVSIAMKSIHRLALSSTHVNLSEIMKNVTGTIMIRVGFGKRYDEDE 1246
KRVQSFR+IRE+EVS +KSI A + VNLS + +T I R FGKR E
Sbjct: 145 SAKRVQSFRYIREEEVSNLVKSIS--ASEGSIVNLSHKIFLMTSGITTRAAFGKRSKHQE 202
Score = 100 bits (250), Expect(4) = 5e-85
Identities = 46/84 (54%), Positives = 58/84 (69%)
Frame = -2
Query: 465 LHGYKIKQKTLVFVNALAIGRDPECWESPEEFLPERFLGSDIEFKGNDFGLIPFGAGRRI 286
++GY+I KT V +N AIGRD W E F PERFL S I+FKG +F IPFGAGRR+
Sbjct: 383 INGYEIPAKTRVAINVWAIGRDERYWAEAESFKPERFLNSTIDFKGTNFEYIPFGAGRRM 442
Query: 285 CPGISMGAVTVDLLLANLVYLFDW 214
CPG++ G ++L LA L+Y FDW
Sbjct: 443 CPGMAFGLSNIELPLAQLLYHFDW 466
Score = 94.4 bits (233), Expect(4) = 5e-85
Identities = 59/201 (29%), Positives = 105/201 (52%), Gaps = 3/201 (1%)
Frame = -3
Query: 1124 VSDLWPGMPFVGLVDRFLGKMDRLEKCFQFFDSFYQELIDEHLNPQNAKSHEEEEDFIDI 945
++D++P + V R K+++L K D Q++I +H N K ++ED +D
Sbjct: 220 IADVYPSVKMFQWVSRAKTKVEKLHK---EIDMILQDIIVDHKNIH--KEESKDEDLVDT 274
Query: 944 LLRLKKDQLFD---LTYDHIKAMLMDVLVAGTDTSSATVVWAMTALIKNPNVMKKAQEEV 774
LL+++++ LT +++KA+++D+ AGT+TSS V+W ++ ++KNP +M+KAQ EV
Sbjct: 275 LLKIQQENDHSHNPLTDNNMKAIILDMFGAGTETSSGAVLWGISEMVKNPKIMEKAQAEV 334
Query: 773 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLPKLTYLKAVVKEIMRLY 594
L +L YLK++++E +RL+
Sbjct: 335 ----------------------------RKVFDKKGYVDETELHQLIYLKSIIRETLRLH 366
Query: 593 PSVPLLVHRETTKDTILHGYK 531
PSVPLLV RE+ + ++GY+
Sbjct: 367 PSVPLLVPRESRERCQINGYE 387
Score = 33.9 bits (76), Expect(4) = 5e-85
Identities = 12/32 (37%), Positives = 23/32 (71%)
Frame = -1
Query: 130 DWGLPDGMRMEDVDFEVLPGLTMHKRNELCLL 35
DW LP+GM+ E++D G+ + ++++LCL+
Sbjct: 465 DWKLPNGMKNEELDMTESFGMAIGRKHDLCLI 496