BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes22_contig00008405
         (1230 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AFO64617.1| cytochrome P450 mono-oxygenase [Artemisia annua]       600   e-169
emb|CAA04116.1| cytochrome P450 [Helianthus tuberosus]                568   e-159
emb|CAA04117.1| cytochrome P450 [Helianthus tuberosus]                568   e-159
ref|XP_002285105.1| PREDICTED: isoflavone 2'-hydroxylase-like [V...   462   e-127
gb|AGC29952.1| CYP81B57 [Sinopodophyllum hexandrum]                   458   e-126

>gb|AFO64617.1| cytochrome P450 mono-oxygenase [Artemisia annua]
          Length = 502

 Score =  600 bits (1547), Expect = e-169
 Identities = 289/356 (81%), Positives = 317/356 (89%)
 Frame = -2

Query: 1211 VRADEGRLLIRKLISECSSPVNLKSVFYELTLNVMMRMISGKRYFGGDIPEVEEEGKRFR 1032
            +R DEG+LL+RKL+S  SS VN+KSVFYELTLNVMMRMISGKRYFGGDIPEVE EGKRFR
Sbjct: 145  IRVDEGKLLVRKLVSNSSSSVNVKSVFYELTLNVMMRMISGKRYFGGDIPEVEAEGKRFR 204

Query: 1031 ELLDETFLLAGAANVGDYLPILSWLGVKRLEKKLIVLRGRKDVFLQGMIEQLRKAKRGEA 852
            E+LDETFLLAGAANVGDYLP L W GV +LEKKL+ LR +++VF QG+IEQLRK K  E 
Sbjct: 205  EILDETFLLAGAANVGDYLPFLRWFGVTKLEKKLVALREKREVFFQGLIEQLRKPKGAEV 264

Query: 851  ENKKKTMIDVLLSLQESDPEYYNDEMIRSLVLVLLSAGTDTTSGTMEWGLSLLLNNPHVL 672
             N KKTMI+VLLSLQESDP+YY DEMIRS VLVLLSAGTDT++GTMEW +SLLLN+P VL
Sbjct: 265  GNNKKTMIEVLLSLQESDPKYYTDEMIRSFVLVLLSAGTDTSAGTMEWAMSLLLNHPQVL 324

Query: 671  HKAQNEIDRHVGKDHLIDESDIANLPYLRCIINETLRLYPAAPFLVPHESSEDCVVGGYN 492
             KAQNEIDR VG D L+DESD+ NLPYLRCIINETLR+ P  P LVPHESSEDCV+GGYN
Sbjct: 325  KKAQNEIDRVVGNDRLVDESDVVNLPYLRCIINETLRICPPGPLLVPHESSEDCVIGGYN 384

Query: 491  IPRGTMLLVNLWAIHHDPKIWTDPERFNPERFEGLEGTRDGFKLMSFGSGRRSCPGEGLA 312
            IPRGTMLLVN WAIHHDPK+WTDPE F PERFEGLEGTRDGFKLM FGSGRRSCPGEGLA
Sbjct: 385  IPRGTMLLVNQWAIHHDPKLWTDPEMFKPERFEGLEGTRDGFKLMPFGSGRRSCPGEGLA 444

Query: 311  MRVLGSTLGSLIQCFDWERVSEKMVDMTEGPGLTMPKVEPLVAKCKPRLEMQNLLS 144
            +RV+GSTLG LIQCFDWER+SEKMVDM+E PGLTMPK EPLVAKCKPRLE+Q LLS
Sbjct: 445  VRVIGSTLGLLIQCFDWERLSEKMVDMSEAPGLTMPKAEPLVAKCKPRLEIQTLLS 500


>emb|CAA04116.1| cytochrome P450 [Helianthus tuberosus]
          Length = 505

 Score =  568 bits (1463), Expect = e-159
 Identities = 274/359 (76%), Positives = 317/359 (88%), Gaps = 3/359 (0%)
 Frame = -2

Query: 1211 VRADEGRLLIRKLISEC---SSPVNLKSVFYELTLNVMMRMISGKRYFGGDIPEVEEEGK 1041
            +R +E RLLI+KL+S C   SS V +K  FYELTLNVMMRMISGKRYFGGD PE+EEEGK
Sbjct: 145  IRVEETRLLIQKLLSACNSGSSQVTMKFSFYELTLNVMMRMISGKRYFGGDNPELEEEGK 204

Query: 1040 RFRELLDETFLLAGAANVGDYLPILSWLGVKRLEKKLIVLRGRKDVFLQGMIEQLRKAKR 861
            RFR++LDETF+LAGA+NVGDYLP+LSWLGVK LEKKLI L+ ++DVF QG+I+QLRK+K 
Sbjct: 205  RFRDMLDETFVLAGASNVGDYLPVLSWLGVKGLEKKLIKLQEKRDVFFQGLIDQLRKSKG 264

Query: 860  GEAENKKKTMIDVLLSLQESDPEYYNDEMIRSLVLVLLSAGTDTTSGTMEWGLSLLLNNP 681
             E  NKKKTMI++LLSLQE++PEYY D MIRS VLVLL+AG+DT++GTMEW +SLLLN+P
Sbjct: 265  TEDVNKKKTMIELLLSLQETEPEYYTDAMIRSFVLVLLAAGSDTSAGTMEWVMSLLLNHP 324

Query: 680  HVLHKAQNEIDRHVGKDHLIDESDIANLPYLRCIINETLRLYPAAPFLVPHESSEDCVVG 501
             VL KAQNEID  +GK+ L+DESDI NLPYLRCIINETLRLYPA P LVPHE+S DCVVG
Sbjct: 325  QVLKKAQNEIDSVIGKNCLVDESDIPNLPYLRCIINETLRLYPAGPLLVPHEASSDCVVG 384

Query: 500  GYNIPRGTMLLVNLWAIHHDPKIWTDPERFNPERFEGLEGTRDGFKLMSFGSGRRSCPGE 321
            GYN+PRGT+L+VN WAIHHDPK+W +PE F PERFEGLEGTRDGFKL+ FGSGRRSCPGE
Sbjct: 385  GYNVPRGTILIVNQWAIHHDPKVWDEPETFKPERFEGLEGTRDGFKLLPFGSGRRSCPGE 444

Query: 320  GLAMRVLGSTLGSLIQCFDWERVSEKMVDMTEGPGLTMPKVEPLVAKCKPRLEMQNLLS 144
            GLA+R+LG TLGS+IQCFDWER SE++VDMTEGPGLTMPK  PLVAKCKPR+EM NLLS
Sbjct: 445  GLAVRMLGMTLGSIIQCFDWERTSEELVDMTEGPGLTMPKAIPLVAKCKPRVEMTNLLS 503


>emb|CAA04117.1| cytochrome P450 [Helianthus tuberosus]
          Length = 520

 Score =  568 bits (1463), Expect = e-159
 Identities = 274/359 (76%), Positives = 317/359 (88%), Gaps = 3/359 (0%)
 Frame = -2

Query: 1211 VRADEGRLLIRKLISEC---SSPVNLKSVFYELTLNVMMRMISGKRYFGGDIPEVEEEGK 1041
            +R +E RLLI+KL+S C   SS V +K  FYELTLNVMMRMISGKRYFGGD PE+EEEGK
Sbjct: 160  IRVEETRLLIQKLLSACNSGSSQVTMKFSFYELTLNVMMRMISGKRYFGGDNPELEEEGK 219

Query: 1040 RFRELLDETFLLAGAANVGDYLPILSWLGVKRLEKKLIVLRGRKDVFLQGMIEQLRKAKR 861
            RFR++LDETF+LAGA+NVGDYLP+LSWLGVK LEKKLI L+ ++DVF QG+I+QLRK+K 
Sbjct: 220  RFRDMLDETFVLAGASNVGDYLPVLSWLGVKGLEKKLIKLQEKRDVFFQGLIDQLRKSKG 279

Query: 860  GEAENKKKTMIDVLLSLQESDPEYYNDEMIRSLVLVLLSAGTDTTSGTMEWGLSLLLNNP 681
             E  NKKKTMI++LLSLQE++PEYY D MIRS VLVLL+AG+DT++GTMEW +SLLLN+P
Sbjct: 280  TEDVNKKKTMIELLLSLQETEPEYYTDAMIRSFVLVLLAAGSDTSAGTMEWVMSLLLNHP 339

Query: 680  HVLHKAQNEIDRHVGKDHLIDESDIANLPYLRCIINETLRLYPAAPFLVPHESSEDCVVG 501
             VL KAQNEID  +GK+ L+DESDI NLPYLRCIINETLRLYPA P LVPHE+S DCVVG
Sbjct: 340  QVLKKAQNEIDSVIGKNCLVDESDIPNLPYLRCIINETLRLYPAGPLLVPHEASSDCVVG 399

Query: 500  GYNIPRGTMLLVNLWAIHHDPKIWTDPERFNPERFEGLEGTRDGFKLMSFGSGRRSCPGE 321
            GYN+PRGT+L+VN WAIHHDPK+W +PE F PERFEGLEGTRDGFKL+ FGSGRRSCPGE
Sbjct: 400  GYNVPRGTILIVNQWAIHHDPKVWDEPETFKPERFEGLEGTRDGFKLLPFGSGRRSCPGE 459

Query: 320  GLAMRVLGSTLGSLIQCFDWERVSEKMVDMTEGPGLTMPKVEPLVAKCKPRLEMQNLLS 144
            GLA+R+LG TLGS+IQCFDWER SE++VDMTEGPGLTMPK  PLVAKCKPR+EM NLLS
Sbjct: 460  GLAVRMLGMTLGSIIQCFDWERTSEELVDMTEGPGLTMPKAIPLVAKCKPRVEMTNLLS 518


>ref|XP_002285105.1| PREDICTED: isoflavone 2'-hydroxylase-like [Vitis vinifera]
          Length = 499

 Score =  462 bits (1188), Expect = e-127
 Identities = 226/356 (63%), Positives = 279/356 (78%)
 Frame = -2

Query: 1211 VRADEGRLLIRKLISECSSPVNLKSVFYELTLNVMMRMISGKRYFGGDIPEVEEEGKRFR 1032
            +R+DE R L+R L    S  V +KS F+E+TLNVMM+MI GKRY+G +I EVEE  + FR
Sbjct: 144  IRSDEVRSLVRWLFKNQSQMVEMKSAFFEMTLNVMMKMIGGKRYYGENIGEVEE-ARMFR 202

Query: 1031 ELLDETFLLAGAANVGDYLPILSWLGVKRLEKKLIVLRGRKDVFLQGMIEQLRKAKRGEA 852
            E++ ETF LAGA N+ D+LPIL WLG+K  E++LI L+ R++ F+Q +IE+ R+ K    
Sbjct: 203  EMVSETFQLAGATNMVDFLPILGWLGLKGTERRLIKLQKRRESFIQNLIEEHRR-KGSNC 261

Query: 851  ENKKKTMIDVLLSLQESDPEYYNDEMIRSLVLVLLSAGTDTTSGTMEWGLSLLLNNPHVL 672
            E ++KTMI+VLLSLQE++PEYY DE+IR L+L +L+ GTDT++GTMEW LSLLLNNP VL
Sbjct: 262  EGRQKTMIEVLLSLQETEPEYYTDEIIRGLMLSMLTGGTDTSAGTMEWALSLLLNNPKVL 321

Query: 671  HKAQNEIDRHVGKDHLIDESDIANLPYLRCIINETLRLYPAAPFLVPHESSEDCVVGGYN 492
             KA  EID  +G D LI+E D+A LPYLR II ETLR+YPA P LVPHESS++C VGG+ 
Sbjct: 322  KKAHQEIDDRLGHDRLIEELDLAQLPYLRSIIKETLRMYPAGPLLVPHESSKECSVGGFR 381

Query: 491  IPRGTMLLVNLWAIHHDPKIWTDPERFNPERFEGLEGTRDGFKLMSFGSGRRSCPGEGLA 312
            IP+GTMLLVNLWAI  D KIW DP  F PERFEG+EG RDGFK + FGSGRR CPGE LA
Sbjct: 382  IPQGTMLLVNLWAIQSDHKIWGDPTEFRPERFEGVEGDRDGFKFVPFGSGRRGCPGEALA 441

Query: 311  MRVLGSTLGSLIQCFDWERVSEKMVDMTEGPGLTMPKVEPLVAKCKPRLEMQNLLS 144
            +R++G  LGSLIQCFDWERV E+MVDMTEG GLT+PK +PL+AKC+PR  M NL S
Sbjct: 442  IRIVGLALGSLIQCFDWERVDEQMVDMTEGGGLTLPKAQPLLAKCRPRPTMVNLFS 497


>gb|AGC29952.1| CYP81B57 [Sinopodophyllum hexandrum]
          Length = 507

 Score =  458 bits (1179), Expect = e-126
 Identities = 219/362 (60%), Positives = 286/362 (79%), Gaps = 6/362 (1%)
 Frame = -2

Query: 1211 VRADEGRLLIRKLISECS-SPVNLKSVFYELTLNVMMRMISGKRYFGGDIPEVEEEGKRF 1035
            +R DE + ++R+L++      V +KSVF+ELTLN+MMRMI+GKRY+G  + ++E+  ++F
Sbjct: 145  IRTDEVKSMVRRLVAGYEYHKVEMKSVFFELTLNIMMRMIAGKRYYGEHVADLEQ-ARKF 203

Query: 1034 RELLDETFLLAGAANVGDYLPILSWLGVKRLEKKLIVLRGRKDVFLQGMIEQLRK----A 867
            +E+++ETF L+GA+NVGD+LP+L W+G K +EK+LI L+ ++D FLQ +I++ RK    +
Sbjct: 204  KEIVEETFELSGASNVGDFLPLLRWIGYKGIEKRLIRLKNKRDEFLQELIQERRKLRVNS 263

Query: 866  KRGEAENKKKTMIDVLLSLQESDPEYYNDEMIRSLVLVLLSAGTDTTSGTMEWGLSLLLN 687
            K   +E KKKT+IDVLLSLQE++PEYY DEMIR L+ +L +AGTDT++GTMEW +SLLLN
Sbjct: 264  KSFPSEEKKKTLIDVLLSLQETEPEYYTDEMIRGLIWILFAAGTDTSAGTMEWAMSLLLN 323

Query: 686  NPHVLHKAQNEIDRHVGKDHLIDESDIANLPYLRCIINETLRLYPAAPFLVPHESSEDCV 507
            NP ++ KAQ+EID  +     IDESD+  LPYL CIINETLR+YPA P L+PHESSEDC 
Sbjct: 324  NPDLIKKAQSEIDLQIEPGRPIDESDLNKLPYLHCIINETLRMYPAGPLLIPHESSEDCE 383

Query: 506  VGGYNIPRGTMLLVNLWAIHHDPKIWTDPERFNPERFEGLE-GTRDGFKLMSFGSGRRSC 330
            +GGYNIP GTMLLVNLWAI +DP +W +P +F PERFEG + G RDGFKLM FGSGRR C
Sbjct: 384  IGGYNIPCGTMLLVNLWAIQNDPNLWKEPRKFKPERFEGYQGGVRDGFKLMPFGSGRRGC 443

Query: 329  PGEGLAMRVLGSTLGSLIQCFDWERVSEKMVDMTEGPGLTMPKVEPLVAKCKPRLEMQNL 150
            PGEGLA RV+G TL SL+QCFDW RV E+MV+M+EG GLT+PK+ PL A C+PR  M N 
Sbjct: 444  PGEGLAXRVVGLTLXSLLQCFDWHRVGEEMVEMSEGTGLTLPKLHPLEAHCRPRSTMLNF 503

Query: 149  LS 144
            LS
Sbjct: 504  LS 505