BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes22_contig00008245
         (1983 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002282161.2| PREDICTED: 3beta-hydroxysteroid-dehydrogenas...   792   0.0  
emb|CBI20743.3| unnamed protein product [Vitis vinifera]              754   0.0  
ref|XP_003522115.1| PREDICTED: 3beta-hydroxysteroid-dehydrogenas...   733   0.0  
ref|XP_002878951.1| 3beta-hydroxysteroid-dehydrogenase/decarboxy...   733   0.0  
ref|XP_003603241.1| 3beta-hydroxysteroid-dehydrogenase/decarboxy...   726   0.0  

>ref|XP_002282161.2| PREDICTED: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform
            2-like [Vitis vinifera]
          Length = 563

 Score =  792 bits (2045), Expect = 0.0
 Identities = 395/554 (71%), Positives = 456/554 (82%)
 Frame = +2

Query: 101  KERWCVVTGGRGFAARHLVDMLIRYEIYSVRIADLAPDIKLETHEEKENLGQALRLGRAQ 280
            +++WCVVTGGRGFAARHLV+MLIR   +SVRIADL P IKLE  EEK  LG+AL+ GRA+
Sbjct: 4    EQKWCVVTGGRGFAARHLVEMLIRSGRFSVRIADLGPSIKLEPSEEKGLLGEALQSGRAE 63

Query: 281  YVSMDLCDKNQVFRACEGAEVVFHMAAPDSSINNYKLQQSVNVQGTKNVIDACTNLNVKR 460
            YVS DL DK QV +AC+GAE VFHMAAPDSSINN++L  SVNVQGTKNVIDAC    VKR
Sbjct: 64   YVSADLRDKAQVLKACQGAEAVFHMAAPDSSINNHQLHHSVNVQGTKNVIDACIEQKVKR 123

Query: 461  LIYTSSPSVVFDGVHGIYNGDESLPYPAKHNDSYSETKAEAEALVIKANGINGLLTCCIR 640
            LIYTSSPSVVFDGVHGI+NGDESLPYP KHNDSYS TKAE EALVIK+NG NGLLTCCIR
Sbjct: 124  LIYTSSPSVVFDGVHGIFNGDESLPYPDKHNDSYSATKAEGEALVIKSNGTNGLLTCCIR 183

Query: 641  PSSIFGPGDKLLVPSLVAAARAGKSKFIIGDGTNMYDFTYVENVAHAHVCAERALASDGS 820
            PSSIFGPGDKLLVPSLV AARAGKSKFIIGDG NMYDFTYVENVAHAH+CAE+ALAS+G 
Sbjct: 184  PSSIFGPGDKLLVPSLVNAARAGKSKFIIGDGNNMYDFTYVENVAHAHICAEQALASEGK 243

Query: 821  ASKRAAGEAYFITNMEPIKFWEFMSLILVGLGYERPRIKIPAAVMMPIAHMVERIYIMLA 1000
             +++AAG+AYFITNMEPIKFWEF+SLIL GLGYERPRIKIPA VMMPIAHMVE  Y MLA
Sbjct: 244  IAEQAAGQAYFITNMEPIKFWEFVSLILEGLGYERPRIKIPALVMMPIAHMVEWTYKMLA 303

Query: 1001 PYGMKVPQLTPSRIRLLTCNRTFNSAKANDRLGYIPIVPLQEGLKRTIESYPDLRAELLP 1180
            PYGMKVPQLTPSRIRLL+C+RTF+ +KA D+L Y PIV LQEGL+RTI+SYPDLRAE LP
Sbjct: 304  PYGMKVPQLTPSRIRLLSCSRTFSCSKAKDQLSYTPIVSLQEGLRRTIDSYPDLRAEHLP 363

Query: 1181 RKEGPSKAAVYLGNEMVADMLLWRDTKLTLTAMLALIVFYVNFVLPGSTMITAISKVLMW 1360
            ++EGPSKA+ YLG+  VAD LLW+D KL LT +L L+ FY NF+  G T++TA+SK+ + 
Sbjct: 364  KREGPSKASRYLGSGRVADTLLWKDKKLALTTLLVLMAFYHNFIASGYTIVTAVSKLFLV 423

Query: 1361 ASIFLFVHGKLPDHIMGYSVEKIPESKFQFSEEMLRRTALSVASSWNCAVNNLKSISSGS 1540
            ASIFLF+HG LP+ I+GY+VEKIP S F FSEE   R ALSVASSWN AVN LKS+  G 
Sbjct: 424  ASIFLFIHGNLPEKILGYTVEKIPPSNFHFSEERSHRAALSVASSWNTAVNVLKSLCKGD 483

Query: 1541 DWMLFFKMALSLFIVSWIGSMSLQNFFVKVLPFSFVVFYVYDQCEDEIDWILQKVFPVER 1720
            DWMLF K+ LSL I+S++G++SLQ+ FV  L  +FV FY+Y+  E+EID +  +      
Sbjct: 484  DWMLFLKVVLSLLILSFLGAISLQSLFVTGLLIAFVAFYIYENKEEEIDRLASEALSFSC 543

Query: 1721 LSNPDFLRLLNPFK 1762
                D  + L  FK
Sbjct: 544  KLKSDIAKKLPTFK 557


>emb|CBI20743.3| unnamed protein product [Vitis vinifera]
          Length = 540

 Score =  754 bits (1947), Expect = 0.0
 Identities = 379/534 (70%), Positives = 436/534 (81%)
 Frame = +2

Query: 161  MLIRYEIYSVRIADLAPDIKLETHEEKENLGQALRLGRAQYVSMDLCDKNQVFRACEGAE 340
            MLIR   +SVRIADL P IKLE  EEK  LG+AL+ GRA+YVS DL DK QV +AC+GAE
Sbjct: 1    MLIRSGRFSVRIADLGPSIKLEPSEEKGLLGEALQSGRAEYVSADLRDKAQVLKACQGAE 60

Query: 341  VVFHMAAPDSSINNYKLQQSVNVQGTKNVIDACTNLNVKRLIYTSSPSVVFDGVHGIYNG 520
             VFHMAAPDSSINN++L  SVNVQGTKNVIDAC    VKRLIYTSSPSVVFDGVHGI+NG
Sbjct: 61   AVFHMAAPDSSINNHQLHHSVNVQGTKNVIDACIEQKVKRLIYTSSPSVVFDGVHGIFNG 120

Query: 521  DESLPYPAKHNDSYSETKAEAEALVIKANGINGLLTCCIRPSSIFGPGDKLLVPSLVAAA 700
            DESLPYP KHNDSYS TKAE EALVIK+NG NGLLTCCIRPSSIFGPGDKLLVPSLV AA
Sbjct: 121  DESLPYPDKHNDSYSATKAEGEALVIKSNGTNGLLTCCIRPSSIFGPGDKLLVPSLVNAA 180

Query: 701  RAGKSKFIIGDGTNMYDFTYVENVAHAHVCAERALASDGSASKRAAGEAYFITNMEPIKF 880
            RAGKSKFIIGDG NMYDFTYVENVAHAH+CAE+ALAS+G  +++AAG+AYFITNMEPIKF
Sbjct: 181  RAGKSKFIIGDGNNMYDFTYVENVAHAHICAEQALASEGKIAEQAAGQAYFITNMEPIKF 240

Query: 881  WEFMSLILVGLGYERPRIKIPAAVMMPIAHMVERIYIMLAPYGMKVPQLTPSRIRLLTCN 1060
            WEF+SLIL GLGYERPRIKIPA VMMPIAHMVE  Y MLAPYGMKVPQLTPSRIRLL+C+
Sbjct: 241  WEFVSLILEGLGYERPRIKIPALVMMPIAHMVEWTYKMLAPYGMKVPQLTPSRIRLLSCS 300

Query: 1061 RTFNSAKANDRLGYIPIVPLQEGLKRTIESYPDLRAELLPRKEGPSKAAVYLGNEMVADM 1240
            RTF+ +KA D+L Y PIV LQEGL+RTI+SYPDLRAE LP++EGPSKA+ YLG+  VAD 
Sbjct: 301  RTFSCSKAKDQLSYTPIVSLQEGLRRTIDSYPDLRAEHLPKREGPSKASRYLGSGRVADT 360

Query: 1241 LLWRDTKLTLTAMLALIVFYVNFVLPGSTMITAISKVLMWASIFLFVHGKLPDHIMGYSV 1420
            LLW+D KL LT +L L+ FY NF+  G T++TA+SK+ + ASIFLF+HG LP+ I+GY+V
Sbjct: 361  LLWKDKKLALTTLLVLMAFYHNFIASGYTIVTAVSKLFLVASIFLFIHGNLPEKILGYTV 420

Query: 1421 EKIPESKFQFSEEMLRRTALSVASSWNCAVNNLKSISSGSDWMLFFKMALSLFIVSWIGS 1600
            EKIP S F FSEE   R ALSVASSWN AVN LKS+  G DWMLF K+ LSL I+S++G+
Sbjct: 421  EKIPPSNFHFSEERSHRAALSVASSWNTAVNVLKSLCKGDDWMLFLKVVLSLLILSFLGA 480

Query: 1601 MSLQNFFVKVLPFSFVVFYVYDQCEDEIDWILQKVFPVERLSNPDFLRLLNPFK 1762
            +SLQ+ FV  L  +FV FY+Y+  E+EID +  +          D  + L  FK
Sbjct: 481  ISLQSLFVTGLLIAFVAFYIYENKEEEIDRLASEALSFSCKLKSDIAKKLPTFK 534


>ref|XP_003522115.1| PREDICTED: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform
            2-like [Glycine max]
          Length = 563

 Score =  733 bits (1893), Expect = 0.0
 Identities = 367/563 (65%), Positives = 448/563 (79%), Gaps = 2/563 (0%)
 Frame = +2

Query: 89   MAAPKERWCVVTGGRGFAARHLVDMLIRYEIYSVRIADLAPDIKLETHEEKENLGQALRL 268
            MAA +++WCVVTGGRGFAARHLV+MLIR+  Y VRIADL  +I LE  E+   LGQAL  
Sbjct: 1    MAAAEDKWCVVTGGRGFAARHLVEMLIRHNEYCVRIADLEANIVLEPAEQLGLLGQALHS 60

Query: 269  GRAQYVSMDLCDKNQVFRACEGAEVVFHMAAPDSSINNYKLQQSVNVQGTKNVIDACTNL 448
            GRAQYVS+DL +K Q+ +A EG EVVFHMAAP+SSINNY+L  SVNVQGTKNVIDAC  L
Sbjct: 61   GRAQYVSLDLRNKAQLLKALEGVEVVFHMAAPNSSINNYQLHHSVNVQGTKNVIDACVEL 120

Query: 449  NVKRLIYTSSPSVVFDGVHGIYNGDESLPYPAKHNDSYSETKAEAEALVIKANGINGLLT 628
            NVKRL+YTSSPSVVFDGVHGI+NG+E++PY    ND YS TKAE EALVIKANG NGLLT
Sbjct: 121  NVKRLVYTSSPSVVFDGVHGIHNGNETMPYAHSPNDHYSATKAEGEALVIKANGTNGLLT 180

Query: 629  CCIRPSSIFGPGDKLLVPSLVAAARAGKSKFIIGDGTNMYDFTYVENVAHAHVCAERALA 808
            CCIRPSSIFGPGD+LLVPSLV AAR GKSKF+IGDG N+YDFTYVENVAHAH+CA+RAL 
Sbjct: 181  CCIRPSSIFGPGDRLLVPSLVDAARKGKSKFLIGDGNNVYDFTYVENVAHAHICADRALV 240

Query: 809  SDGSASKRAAGEAYFITNMEPIKFWEFMSLILVGLGYERPRIKIPAAVMMPIAHMVERIY 988
            S+   S++AAGEAYFITNMEP+KFWEF+S+++ GLGYE PRIKIP  V+MP AH+VE IY
Sbjct: 241  SEAPVSEKAAGEAYFITNMEPMKFWEFVSVVVEGLGYEGPRIKIPTFVIMPFAHLVEWIY 300

Query: 989  IMLAPYGMKVPQLTPSRIRLLTCNRTFNSAKANDRLGYIPIVPLQEGLKRTIESYPDLRA 1168
             +L PYGMKVPQLTPSRIRL +C+RTF+ +KA DRLGY PIV LQEGL+RTIESY  L+A
Sbjct: 301  RLLGPYGMKVPQLTPSRIRLTSCSRTFDCSKAKDRLGYAPIVTLQEGLRRTIESYTHLKA 360

Query: 1169 ELLP--RKEGPSKAAVYLGNEMVADMLLWRDTKLTLTAMLALIVFYVNFVLPGSTMITAI 1342
            E  P  ++EG SKA++YLG+  VAD LLW+D K T  A+L LI  Y NF+   +T+I+A+
Sbjct: 361  ENQPKTKREGSSKASIYLGSGRVADTLLWKDKKQTFIALLVLIAIYFNFIASENTIISAL 420

Query: 1343 SKVLMWASIFLFVHGKLPDHIMGYSVEKIPESKFQFSEEMLRRTALSVASSWNCAVNNLK 1522
            +K+L++ASIFLF+HG LP  I+GY++EKIP+S F  SE+M  + ALSVASSWN AVN LK
Sbjct: 421  TKLLLFASIFLFIHGILPAKILGYTLEKIPKSWFHLSEDMSHQIALSVASSWNIAVNVLK 480

Query: 1523 SISSGSDWMLFFKMALSLFIVSWIGSMSLQNFFVKVLPFSFVVFYVYDQCEDEIDWILQK 1702
            S++ G+DW+LFFK  LSLFI+S++G+ SLQ+ +   + F+F+ FYVY+Q E+EI  +  K
Sbjct: 481  SLADGNDWVLFFKAVLSLFILSFLGAFSLQSLYTIGVTFAFIAFYVYEQKEEEIGGLFIK 540

Query: 1703 VFPVERLSNPDFLRLLNPFKPLD 1771
               +      D  R     K +D
Sbjct: 541  THSLGCKLKSDLTRKFLSSKKID 563


>ref|XP_002878951.1| 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2
            [Arabidopsis lyrata subsp. lyrata]
            gi|297324790|gb|EFH55210.1|
            3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform
            2 [Arabidopsis lyrata subsp. lyrata]
          Length = 564

 Score =  733 bits (1892), Expect = 0.0
 Identities = 367/548 (66%), Positives = 434/548 (79%), Gaps = 2/548 (0%)
 Frame = +2

Query: 92   AAPKERWCVVTGGRGFAARHLVDMLIRYEIYSVRIADLAPDIKLETHEEKENLGQALRLG 271
            A   ERWCVVTGGRGFAARHLV+ML+RYE++SVRIADLAP I L+ HEE   L + LR G
Sbjct: 5    ATENERWCVVTGGRGFAARHLVEMLVRYEMFSVRIADLAPAIMLDLHEENGVLDEGLRSG 64

Query: 272  RAQYVSMDLCDKNQVFRACEGAEVVFHMAAPDSSINNYKLQQSVNVQGTKNVIDACTNLN 451
            R QY+S DL DK+QV +A +GAEVVFHMAAPDSSINN++LQ SVNVQGT+NVIDAC ++ 
Sbjct: 65   RVQYISADLRDKSQVVKAFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTQNVIDACVDVG 124

Query: 452  VKRLIYTSSPSVVFDGVHGIYNGDESLPYPAKHNDSYSETKAEAEALVIKANGINGLLTC 631
            VKRLIYTSSPSVVFDGVHGI NG ES+ YP KHNDSYS TKAE E L++KANG  GLLTC
Sbjct: 125  VKRLIYTSSPSVVFDGVHGILNGSESMAYPIKHNDSYSATKAEGEELIMKANGRKGLLTC 184

Query: 632  CIRPSSIFGPGDKLLVPSLVAAARAGKSKFIIGDGTNMYDFTYVENVAHAHVCAERALAS 811
            CIRPSSIFGPGD+LLVPSLVAAARAGKSKFIIGDG N+YDFTYVENVAHAHVCAERALAS
Sbjct: 185  CIRPSSIFGPGDRLLVPSLVAAARAGKSKFIIGDGNNLYDFTYVENVAHAHVCAERALAS 244

Query: 812  DGSASKRAAGEAYFITNMEPIKFWEFMSLILVGLGYERPRIKIPAAVMMPIAHMVERIYI 991
             G  S +AAG+AYFITNMEPIKFWEF+S +L GLGYERP IKIPA VMMPIAH+VE  Y 
Sbjct: 245  GGDVSTKAAGQAYFITNMEPIKFWEFVSQLLEGLGYERPSIKIPAFVMMPIAHLVELTYK 304

Query: 992  MLAPYGMKVPQLTPSRIRLLTCNRTFNSAKANDRLGYIPIVPLQEGLKRTIESYPDLRAE 1171
            +L PYGMKVPQLTPSR+RLL+C+RTF+S KA DRLGY P+VPLQEG++RTI+S+  L A 
Sbjct: 305  LLGPYGMKVPQLTPSRVRLLSCSRTFDSTKAKDRLGYAPVVPLQEGIRRTIDSFSHLTAG 364

Query: 1172 LLPRKEGPSKAAVYLGNEMVADMLLWRDTKLTLTAMLALIVFYVNFVLPGSTMITAISKV 1351
               ++EGPSKA+  LG   VAD LLW+D K TL A+  LI  Y NFV  GST++TA+SK 
Sbjct: 365  SQSKREGPSKASRILGGGKVADTLLWKDLKQTLIAIFILISVYYNFVATGSTIVTALSKA 424

Query: 1352 LMWASIFLFVHGKLPDHIMGYSVEKIPESKFQFSEEMLRRTALSVASSWNCAVNNLKSIS 1531
            L+ AS+FLF+HG LP+ I GY+VEKIP S+F  S++     +LSV SSWN  V  L+S+ 
Sbjct: 425  LLVASVFLFLHGILPEKIFGYTVEKIPASQFHLSKDSSEHLSLSVISSWNTTVKALRSLC 484

Query: 1532 SGSDWMLFFKMALSLFIVSWIGSMSLQNFFVKVLPFSFVVFYVYDQCEDEIDWILQ--KV 1705
             G+DW  FFK+   L  +S  G++SL N FV  +P +F+ F VY++ E EID I+   K 
Sbjct: 485  QGNDWSFFFKVVFVLLALSLAGAISLHNIFVIGIPIAFIAFIVYEKKEQEIDSIVVSFKS 544

Query: 1706 FPVERLSN 1729
            F  +R S+
Sbjct: 545  FACKRKSD 552


>ref|XP_003603241.1| 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform [Medicago
            truncatula] gi|355492289|gb|AES73492.1|
            3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform
            [Medicago truncatula]
          Length = 579

 Score =  726 bits (1873), Expect = 0.0
 Identities = 363/557 (65%), Positives = 438/557 (78%), Gaps = 19/557 (3%)
 Frame = +2

Query: 89   MAAPKERWCVVTGGRGFAARHLVDMLIRYEIYSVRIADLAPDIKLETHEEKENLGQALRL 268
            MAA +E WCVVTGGRGFAARHLV+MLI+   Y VRIADL   I+LE  E+   LGQAL  
Sbjct: 1    MAAAEEGWCVVTGGRGFAARHLVEMLIQLNTYCVRIADLGSTIELEPSEQLGLLGQALHS 60

Query: 269  GRAQYVSMDLCDKNQVFRACEGAEVVFHMAAPDSSINNYKLQQSVNVQGTKNVIDACTNL 448
            GRAQYVS+DL +K Q+ +A +G EVVFHMAAP+SSINNY+L  SVNV+GTKNVIDAC  L
Sbjct: 61   GRAQYVSVDLRNKPQLLKAFDGVEVVFHMAAPNSSINNYQLHHSVNVEGTKNVIDACVEL 120

Query: 449  NVKRLIYTSSPSVVFDGVHGIYNGDESLPYPAKHNDSYSETKAEAEALVIKANGINGLLT 628
             VKRLIYTSSPSVVFDG+HGI+NG ESLPYP  HND YS TKAE E LVIKANG +GLLT
Sbjct: 121  KVKRLIYTSSPSVVFDGIHGIHNGSESLPYPPSHNDHYSATKAEGEGLVIKANGTSGLLT 180

Query: 629  CCIRPSSIFGPGDKLLVPSLVAAARAGKSKFIIGDGTNMYDFTYVENVAHAHVCAERALA 808
            CCIRPSSIFGPGDKLLVPSLV AA+AGKSKFI+GDG N+YDFTYVENVAHAH+CA+RALA
Sbjct: 181  CCIRPSSIFGPGDKLLVPSLVDAAKAGKSKFIVGDGNNVYDFTYVENVAHAHICADRALA 240

Query: 809  SDGSASKRAAGEAYFITNMEPIKFWEFMSLILVGLGYERPRIKIPAAVMMPIAHMVERIY 988
            S+G+ S++AAGEAYFITNMEPIKFWEFMSLIL GLGY+RP IKIP  V+MPIAH+VE IY
Sbjct: 241  SEGTVSEKAAGEAYFITNMEPIKFWEFMSLILEGLGYQRPSIKIPVFVIMPIAHLVEWIY 300

Query: 989  IMLAPYGMKVPQLTPSRIRLLTCNRTFNSAKANDRLGYIPIVPLQEGLKRTIESYPDLRA 1168
             +L PYGMKVPQLTPSRIRL +C R+F+ +KA DRL Y PI+PLQEG++RTIESYP LRA
Sbjct: 301  RLLGPYGMKVPQLTPSRIRLTSCTRSFDCSKAKDRLDYAPIIPLQEGIRRTIESYPHLRA 360

Query: 1169 E--LLPRKEGPSKAAVYLGNEMVADMLLWRDTKLTLTAMLALIVFYVNFVLPGSTMITAI 1342
            E  L  ++EGPSKA+VYLG+  VAD LLW+D K T T +L  I  YVNF+   +T ITA+
Sbjct: 361  ENQLKSKREGPSKASVYLGSGRVADTLLWKDKKQTFTTLLVFIAIYVNFIASENTFITAL 420

Query: 1343 SKVLMWASIFLFVHGKLPDHIMGYSVEKIPESKFQFSEEMLRRTALSVASSWNCAVNNLK 1522
            +K+L+++SI LF+HG LP  I+GY+VEK+P S F  SE++  + A SVASSWN AVN LK
Sbjct: 421  TKLLLYSSIGLFIHGILPAKILGYTVEKMPTSWFHLSEDISNQIAFSVASSWNFAVNALK 480

Query: 1523 SISSGSDWMLFFKMALSLFIVSWIGSMSLQNFF-----------------VKVLPFSFVV 1651
            S++ G+ W++FFK+  +L ++S++G+ SL+N +                 V  L   F  
Sbjct: 481  SLAEGNSWVMFFKVVFTLLVLSFLGAFSLKNLYTIGSLCLDENDTSLFLSVTGLTLGFTA 540

Query: 1652 FYVYDQCEDEIDWILQK 1702
            FYVY++ E++ID I  K
Sbjct: 541  FYVYEKKEEDIDGIFLK 557