BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes22_contig00007871
         (1622 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002267958.1| PREDICTED: 3-epi-6-deoxocathasterone 23-mono...   652   0.0  
ref|XP_002310278.1| predicted protein [Populus trichocarpa] gi|2...   648   0.0  
emb|CAN73509.1| hypothetical protein VITISV_007910 [Vitis vinifera]   640   0.0  
ref|XP_003520275.1| PREDICTED: 3-epi-6-deoxocathasterone 23-mono...   622   e-175
ref|NP_568002.1| 3-epi-6-deoxocathasterone 23-monooxygenase [Ara...   616   e-174

>ref|XP_002267958.1| PREDICTED: 3-epi-6-deoxocathasterone 23-monooxygenase [Vitis
            vinifera] gi|297735073|emb|CBI17435.3| unnamed protein
            product [Vitis vinifera]
          Length = 486

 Score =  652 bits (1682), Expect = 0.0
 Identities = 316/453 (69%), Positives = 387/453 (85%), Gaps = 1/453 (0%)
 Frame = +2

Query: 170  PRGTSGWPFIGETIEFIASGYTSRPVTFMEKRKSLYGKVFKTHILGRPIIVSTDPDVNKV 349
            PRG  GWP IGET++FIASG++SRPV+FMEKRKS YGKVFKT+ILG+P+IVSTDP+VNKV
Sbjct: 35   PRGNPGWPVIGETLDFIASGWSSRPVSFMEKRKSRYGKVFKTNILGKPVIVSTDPEVNKV 94

Query: 350  VLQNQGNVFIPCYPKSVIELLGESSILQINGGLQKRLHAIIGGFLRSPQFKARITKDIEN 529
            VLQN GNVFIP YP+++ E+LGESSILQ+NG LQKR+HA++GGFLRSPQ KARIT+DIE 
Sbjct: 95   VLQNIGNVFIPAYPQTITEILGESSILQMNGSLQKRVHALLGGFLRSPQLKARITRDIER 154

Query: 530  SVKLTLSSWMDHKNSLQPLYLQDETKKITFEILVRVLMSVEPGEDMEFLKREFMEVIKGL 709
             VKLTL SW D       +Y+QDE +KITF++LVRVL+S+ PGED+ F+KREF EVIKGL
Sbjct: 155  YVKLTLDSWKDQ----HIVYVQDEVRKITFDVLVRVLVSITPGEDLNFMKREFAEVIKGL 210

Query: 710  ICLPIKLPGFRMYKSLQAKERMLKMVRKIVDDRKWAMEKIEMKG-SGSPNDAIDVLLRDT 886
            ICLPIKLPG R+YKSL+AKER+LK+VR+IV++RK AM++ + +   G PNDA+DVLLRDT
Sbjct: 211  ICLPIKLPGTRLYKSLKAKERLLKLVRRIVEERKAAMDRGDHETLKGPPNDAVDVLLRDT 270

Query: 887  GESDGTQQRLPVDFISGNIIEMMIPGEDSVPMIMTLAIKYLSDNPAALACLVDENDELKK 1066
            G+S  T  RL +DFIS N+IEMMIPGE+++P  MTLA+K+LSD P AL  L++EN ELKK
Sbjct: 271  GDSSETS-RLSLDFISSNMIEMMIPGEETLPTAMTLAVKFLSDCPVALNQLMEENMELKK 329

Query: 1067 RKDESCKEYSWTDYVSLPFTQGVISETLRMANIINAIWRKALEDVEIKGYLIPKGWCVLA 1246
             K  S ++Y+WTDY+ LPFTQ VI+ETLRMANIINA+WRKA++DV+IK YLIP+GW V+A
Sbjct: 330  GKALSGEDYAWTDYMCLPFTQNVINETLRMANIINAVWRKAVKDVKIKDYLIPEGWGVMA 389

Query: 1247 SLTSVHMDEENYENPDEFDPWRWEKTGASVNSNKFTPFGGGQRLCPGLEFSRLEISIFLH 1426
            S TS+HMDEENYENP +F+PWRWEK  +  N+N +TPFGGGQRLCPGLE S+LEISIFLH
Sbjct: 390  SFTSIHMDEENYENPYQFNPWRWEKAASVNNNNSYTPFGGGQRLCPGLELSKLEISIFLH 449

Query: 1427 HLVTTYTWVIEEDQIVYFPTVKTRRKLPIIVTP 1525
            HLVTTY WV ++D +VYFPTVK R+KLPI VTP
Sbjct: 450  HLVTTYRWVAKKDDVVYFPTVKMRKKLPITVTP 482


>ref|XP_002310278.1| predicted protein [Populus trichocarpa] gi|222853181|gb|EEE90728.1|
            predicted protein [Populus trichocarpa]
          Length = 463

 Score =  648 bits (1672), Expect = 0.0
 Identities = 315/453 (69%), Positives = 380/453 (83%), Gaps = 2/453 (0%)
 Frame = +2

Query: 170  PRGTSGWPFIGETIEFIASGYTSRPVTFMEKRKSLYGKVFKTHILGRPIIVSTDPDVNKV 349
            P+G+ GWP IGET++FIA+GYTS+PV+FMEKR+SLYGKVFKTHILG PIIVSTDP VNKV
Sbjct: 9    PKGSFGWPLIGETLDFIAAGYTSQPVSFMEKRRSLYGKVFKTHILGTPIIVSTDPAVNKV 68

Query: 350  VLQNQGNVFIPCYPKSVIELLGESSILQINGGLQKRLHAIIGGFLRSPQFKARITKDIEN 529
            VLQN GN+FIP YPKSV E+ GE SILQ NG LQK++HA+IGGFLRSPQFK RITKDIE+
Sbjct: 69   VLQNHGNIFIPAYPKSVREIFGEYSILQTNGTLQKKVHALIGGFLRSPQFKTRITKDIEH 128

Query: 530  SVKLTLSSWMDHKNSLQPLYLQDETKKITFEILVRVLMSVEPGEDMEFLKREFMEVIKGL 709
             VKLTL+SW D    L  L++Q+ET+K TF++LV+ L+S++P +D++FLKREF E IKGL
Sbjct: 129  HVKLTLTSWKD----LPLLFVQEETQKFTFKVLVKALLSLDPSDDLDFLKREFDEFIKGL 184

Query: 710  ICLPIKLPGFRMYKSLQAKERMLKMVRKIVDDRKWAMEKIEMKGSGS--PNDAIDVLLRD 883
            ICLPIKLPG R+YKSL+AKER+ K+V+KIV+ RK  +EK E +   +  PNDA+DVLLRD
Sbjct: 185  ICLPIKLPGTRLYKSLKAKERIFKLVKKIVEQRKLGLEKSEDQEHQNTIPNDAVDVLLRD 244

Query: 884  TGESDGTQQRLPVDFISGNIIEMMIPGEDSVPMIMTLAIKYLSDNPAALACLVDENDELK 1063
                   +Q L +DFI  +IIE+MIPGE++VPM MTLA+K+LSD P AL  L +EN +LK
Sbjct: 245  ISGEQNDKQSLSLDFIISHIIELMIPGEETVPMSMTLAVKFLSDCPVALELLTEENMKLK 304

Query: 1064 KRKDESCKEYSWTDYVSLPFTQGVISETLRMANIINAIWRKALEDVEIKGYLIPKGWCVL 1243
            ++K +S  +Y WTDY+SL FTQ VI+ETLR+ANIINA+WRKAL+DVEIKG LIP+GWCVL
Sbjct: 305  RQKTDSGDDYCWTDYMSLQFTQNVINETLRVANIINAVWRKALKDVEIKGILIPEGWCVL 364

Query: 1244 ASLTSVHMDEENYENPDEFDPWRWEKTGASVNSNKFTPFGGGQRLCPGLEFSRLEISIFL 1423
             S +SVHMDEENYENP EFDPWRWEKTGASV +N FTPFGGGQRLCPGLE SRLEISIFL
Sbjct: 365  TSFSSVHMDEENYENPHEFDPWRWEKTGASVKNNCFTPFGGGQRLCPGLELSRLEISIFL 424

Query: 1424 HHLVTTYTWVIEEDQIVYFPTVKTRRKLPIIVT 1522
            HHLVTTY W+ E+D I+YFPTVK ++KLPI VT
Sbjct: 425  HHLVTTYRWIAEKDDIIYFPTVKLKKKLPIRVT 457


>emb|CAN73509.1| hypothetical protein VITISV_007910 [Vitis vinifera]
          Length = 501

 Score =  640 bits (1651), Expect = 0.0
 Identities = 315/468 (67%), Positives = 386/468 (82%), Gaps = 16/468 (3%)
 Frame = +2

Query: 170  PRGTSGWPFIGETIEFIASGYTSRPVTFMEKRKSLYGKVFKTHILGRPIIVSTDPDVNKV 349
            PRG  GWP IGET++FIASG++SRPV+FMEKRKS YGKVFKT+ILG+P+IVSTDP+VNKV
Sbjct: 35   PRGNPGWPVIGETLDFIASGWSSRPVSFMEKRKSRYGKVFKTNILGKPVIVSTDPEVNKV 94

Query: 350  VLQNQGNVFIPCYPKSVIELLGESSILQINGGLQKRLHAIIGGFLRSPQFKARITKDIEN 529
            VLQN GN FIP YP+++ E+LGESSILQ+NG LQKR+HA++GGFLRSPQ KARIT+DIE 
Sbjct: 95   VLQNIGNXFIPAYPQTITEILGESSILQMNGSLQKRVHALLGGFLRSPQLKARITRDIER 154

Query: 530  SVKLTLSSWMDHKNSLQPLYLQDETKKITFEILVRVLMSVEPGEDMEFLKREFMEVIKGL 709
             VKLTL SW D       +Y+QDE +KITF++LVRVL+S+ PGED+ F+KREF EVIKGL
Sbjct: 155  YVKLTLDSWKDQ----HIVYVQDEVRKITFDVLVRVLVSITPGEDLNFMKREFAEVIKGL 210

Query: 710  ICLPIKLPGFRMYKSLQAKERMLKMVRKIVDDRKWAMEKIEMKG-SGSPNDAIDVLLRDT 886
            ICLPIKLPG R+YKSL+AKER+LK+VR+IV++RK AM++ + +   G PNDA+DVLLRDT
Sbjct: 211  ICLPIKLPGTRLYKSLKAKERLLKLVRRIVEERKAAMDRGDHETLKGPPNDAVDVLLRDT 270

Query: 887  GESDGTQQRLPVDFISGNIIEMMIPGEDSVPMIMTLAIKYLSDNPAALACLVDENDELKK 1066
            G+S  T  RL +DFIS N+IEMMIPGE+++P  MTLA+K+LSD P AL  L++EN ELKK
Sbjct: 271  GDSSETS-RLSLDFISSNMIEMMIPGEETLPTAMTLAVKFLSDCPVALNQLMEENMELKK 329

Query: 1067 RKDESCKEYSWTDYVSLPFTQGV---------------ISETLRMANIINAIWRKALEDV 1201
             K  S ++Y+WTDY+ LPFTQ V               I+ETLRMANIINA+WRKA++DV
Sbjct: 330  GKALSGEDYAWTDYMCLPFTQNVGSSLNMRHCEVEWKVINETLRMANIINAVWRKAVKDV 389

Query: 1202 EIKGYLIPKGWCVLASLTSVHMDEENYENPDEFDPWRWEKTGASVNSNKFTPFGGGQRLC 1381
            +IK YLIP+GW V+AS TS+HMDEENYENP +F+PWRWEK  +  N+N +TPFGGGQRLC
Sbjct: 390  KIKDYLIPEGWGVMASFTSIHMDEENYENPYQFNPWRWEKAASVNNNNSYTPFGGGQRLC 449

Query: 1382 PGLEFSRLEISIFLHHLVTTYTWVIEEDQIVYFPTVKTRRKLPIIVTP 1525
            PGLE S+LEISIFLHHLVTTY WV ++D +VYFPTVK R+KLPI VTP
Sbjct: 450  PGLELSKLEISIFLHHLVTTYXWVAKKDDVVYFPTVKMRKKLPITVTP 497


>ref|XP_003520275.1| PREDICTED: 3-epi-6-deoxocathasterone 23-monooxygenase-like [Glycine
            max]
          Length = 486

 Score =  622 bits (1603), Expect = e-175
 Identities = 304/496 (61%), Positives = 390/496 (78%), Gaps = 2/496 (0%)
 Frame = +2

Query: 41   MTWVFGIWMLLGCLFYKYLMLRRRGLPDMEQSGGDGLRRRLQPPRGTSGWPFIGETIEFI 220
            M W+ G+W L+G L   + +  R+     E       +++ + P+G +GWP +GET+EFI
Sbjct: 1    MEWIIGLWTLMGFLLCWWFLCVRK-----ENQNSKVKKKKGKVPKGNTGWPLLGETLEFI 55

Query: 221  ASGYTSRPVTFMEKRKSLYGKVFKTHILGRPIIVSTDPDVNKVVLQNQGNVFIPCYPKSV 400
            A GYTS PV+FMEKRKSLYG VFKT ILG  +IVSTDP+VNKV+LQNQGN+F+P YPKSV
Sbjct: 56   ACGYTSNPVSFMEKRKSLYGSVFKTSILGTGVIVSTDPEVNKVILQNQGNIFVPAYPKSV 115

Query: 401  IELLGESSILQINGGLQKRLHAIIGGFLRSPQFKARITKDIENSVKLTLSSWMDHKNSLQ 580
             EL+GE SILQ+NG + +++H+++GGFLRSPQFKARIT+DIE+SVK   ++W       Q
Sbjct: 116  RELMGEHSILQMNGNMHRKIHSLLGGFLRSPQFKARITRDIEHSVKQCFATWTH-----Q 170

Query: 581  PL-YLQDETKKITFEILVRVLMSVEPGEDMEFLKREFMEVIKGLICLPIKLPGFRMYKSL 757
            P+ YLQD+ KKITF ILV+VL+S+ PGED++FLKREF E IKGLICLP+K+PG R+YKSL
Sbjct: 171  PIIYLQDQVKKITFTILVKVLLSIGPGEDLDFLKREFEEFIKGLICLPLKIPGTRLYKSL 230

Query: 758  QAKERMLKMVRKIVDDRKWAMEKIEMKGS-GSPNDAIDVLLRDTGESDGTQQRLPVDFIS 934
            +AKERM+K+VR+++++R   M       +  S ND +DVLLRD G+++     L  + I 
Sbjct: 231  KAKERMMKIVRRVIEERINNMRNNNNSNNKDSANDVVDVLLRDIGDTNSISNML--ENIC 288

Query: 935  GNIIEMMIPGEDSVPMIMTLAIKYLSDNPAALACLVDENDELKKRKDESCKEYSWTDYVS 1114
             NIIEMMIPGE+++P  MT+++K+LS+ P AL+ L++EN ELK+RK+ S  +Y+W DY+S
Sbjct: 289  ENIIEMMIPGEETLPTAMTMSVKFLSNYPVALSKLLEENMELKRRKNNS-DDYAWNDYLS 347

Query: 1115 LPFTQGVISETLRMANIINAIWRKALEDVEIKGYLIPKGWCVLASLTSVHMDEENYENPD 1294
            LPFTQ VISE+LRMANI+NAIWRKA++DV+IKGYLIPK WCV+ASLTSVHMD  NYENP 
Sbjct: 348  LPFTQNVISESLRMANIVNAIWRKAVKDVDIKGYLIPKDWCVVASLTSVHMDGMNYENPF 407

Query: 1295 EFDPWRWEKTGASVNSNKFTPFGGGQRLCPGLEFSRLEISIFLHHLVTTYTWVIEEDQIV 1474
            EF+P RWE  G   N+N FTPFGGGQRLCPG+E SRLE+SIFLHHLVTTY WV EED+I+
Sbjct: 408  EFNPGRWENIGTGTNNNCFTPFGGGQRLCPGIELSRLELSIFLHHLVTTYRWVAEEDEII 467

Query: 1475 YFPTVKTRRKLPIIVT 1522
            YFPTVK +RKLPI VT
Sbjct: 468  YFPTVKMKRKLPISVT 483


>ref|NP_568002.1| 3-epi-6-deoxocathasterone 23-monooxygenase [Arabidopsis thaliana]
            gi|150421525|sp|Q9M066.3|C90C1_ARATH RecName:
            Full=3-epi-6-deoxocathasterone 23-monooxygenase; AltName:
            Full=Cytochrome P450 90C1; AltName: Full=Protein
            ROTUNDIFOLIA 3 gi|115646893|gb|ABJ17155.1| At4g36380
            [Arabidopsis thaliana] gi|332661249|gb|AEE86649.1|
            3-epi-6-deoxocathasterone 23-monooxygenase [Arabidopsis
            thaliana]
          Length = 524

 Score =  616 bits (1589), Expect = e-174
 Identities = 309/482 (64%), Positives = 376/482 (78%), Gaps = 1/482 (0%)
 Frame = +2

Query: 92   YLMLRRRGLPDMEQSGGDGLRRRLQPPRGTSGWPFIGETIEFIASGYTSRPVTFMEKRKS 271
            +L LR     D ++   +  +++   P G+ GWP IGET+ FIA GY+SRPVTFM+KRKS
Sbjct: 44   WLRLRNSKTKDGDEEEDNEEKKKGMIPNGSLGWPVIGETLNFIACGYSSRPVTFMDKRKS 103

Query: 272  LYGKVFKTHILGRPIIVSTDPDVNKVVLQNQGNVFIPCYPKSVIELLGESSILQINGGLQ 451
            LYGKVFKT+I+G PII+STD +VNKVVLQN GN F+P YPKS+ ELLGE+SIL ING  Q
Sbjct: 104  LYGKVFKTNIIGTPIIISTDAEVNKVVLQNHGNTFVPAYPKSITELLGENSILSINGPHQ 163

Query: 452  KRLHAIIGGFLRSPQFKARITKDIENSVKLTLSSWMDHKNSLQPLYLQDETKKITFEILV 631
            KRLH +IG FLRSP  K RIT+DIE SV LTL+SW      L  +++QDE KK+TFEILV
Sbjct: 164  KRLHTLIGAFLRSPHLKDRITRDIEASVVLTLASWAQ----LPLVHVQDEIKKMTFEILV 219

Query: 632  RVLMSVEPGEDMEFLKREFMEVIKGLICLPIKLPGFRMYKSLQAKERMLKMVRKIVDDRK 811
            +VLMS  PGEDM  LK EF E IKGLIC+PIK PG R+YKSL+AKER++KMV+K+V++R+
Sbjct: 220  KVLMSTSPGEDMNILKLEFEEFIKGLICIPIKFPGTRLYKSLKAKERLIKMVKKVVEERQ 279

Query: 812  WAMEKIEMKGSGSPNDAIDVLLRDTGESDGTQQRLPVDFISGNIIEMMIPGEDSVPMIMT 991
             AM       +   ND +DVLLRD G+S+  +Q  P DF+SG I+EMMIPGE+++P  MT
Sbjct: 280  VAMTT-----TSPANDVVDVLLRDGGDSE--KQSQPSDFVSGKIVEMMIPGEETMPTAMT 332

Query: 992  LAIKYLSDNPAALACLVDENDELKKRKDESCKEYSWTDYVSLPFTQGVISETLRMANIIN 1171
            LA+K+LSDNP ALA LV+EN E+K+RK E  +EY WTDY+SL FTQ VI+ETLRMANIIN
Sbjct: 333  LAVKFLSDNPVALAKLVEENMEMKRRKLELGEEYKWTDYMSLSFTQNVINETLRMANIIN 392

Query: 1172 AIWRKALEDVEIKGYLIPKGWCVLASLTSVHMDEENYENPDEFDPWRWEKTGASVNSN-K 1348
             +WRKAL+DVEIKGYLIPKGWCVLAS  SVHMDE+ Y+NP +FDPWRW++   S NS+  
Sbjct: 393  GVWRKALKDVEIKGYLIPKGWCVLASFISVHMDEDIYDNPYQFDPWRWDRINGSANSSIC 452

Query: 1349 FTPFGGGQRLCPGLEFSRLEISIFLHHLVTTYTWVIEEDQIVYFPTVKTRRKLPIIVTPR 1528
            FTPFGGGQRLCPGLE S+LEISIFLHHLVT Y+W  EED+IV FPTVK +R+LPI V   
Sbjct: 453  FTPFGGGQRLCPGLELSKLEISIFLHHLVTRYSWTAEEDEIVSFPTVKMKRRLPIRVATV 512

Query: 1529 SD 1534
             D
Sbjct: 513  DD 514