BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes22_contig00007558
         (1983 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002312060.1| predicted protein [Populus trichocarpa] gi|2...   785   0.0  
ref|XP_002526791.1| ankyrin repeat-containing protein, putative ...   782   0.0  
ref|NP_187842.1| ankyrin repeats-containing protein [Arabidopsis...   764   0.0  
ref|XP_004149816.1| PREDICTED: ankyrin repeat-containing protein...   759   0.0  
ref|XP_002282204.1| PREDICTED: ankyrin repeat-containing protein...   757   0.0  

>ref|XP_002312060.1| predicted protein [Populus trichocarpa] gi|222851880|gb|EEE89427.1|
            predicted protein [Populus trichocarpa]
          Length = 588

 Score =  785 bits (2027), Expect = 0.0
 Identities = 432/591 (73%), Positives = 478/591 (80%), Gaps = 6/591 (1%)
 Frame = -2

Query: 1865 MGSAIDEG---DLEKGLMTPSKQKQNSNPLREXXXXXXXXXXXXXA-LVLTNSGKRIDQA 1698
            M S ++EG   DLEKGL+ P   + N NPL +               LVL+NSGKRIDQA
Sbjct: 1    MASPMEEGSERDLEKGLVQP---QLNQNPLTDPSPTPSPSSTSTAPALVLSNSGKRIDQA 57

Query: 1697 GKKKYVKQVTGRHNDTELHLAAQRGDLAAVKQILDDINSQMVGTLSGADFDAEVAEIRAS 1518
            GKKKYVKQVTGRHNDTELHLAAQRG+LA V+ IL+DINSQMVGTLSGADFD EVAEIRAS
Sbjct: 58   GKKKYVKQVTGRHNDTELHLAAQRGNLADVQHILNDINSQMVGTLSGADFDTEVAEIRAS 117

Query: 1517 VVNEVNELGETALYTAAERGHLEVVKELLKYSDKETISRRSRLEFDPLHIAASQGHHAVV 1338
            VVNEVNELGETAL+TAA++GHLEVVKELLKYS+KE ++R++R  +DPLHIAA QGHHA+V
Sbjct: 118  VVNEVNELGETALFTAADKGHLEVVKELLKYSNKECLTRKNRSGYDPLHIAAVQGHHAIV 177

Query: 1337 QLLLDHDVSLCQTRSQGNATPLITAASKGHTAVVHELLSKDPSLLDIPRSNGKNALHLAA 1158
            Q+LLDHD SL QT    NATPL++AA++GHTAVV ELLSKD SLL+I RSNGKNALHLAA
Sbjct: 178  QVLLDHDPSLSQTHGPSNATPLVSAATRGHTAVVIELLSKDGSLLEISRSNGKNALHLAA 237

Query: 1157 RSGHVETLKALLDKDPQLARRTDKKGQTALHMAVKGASSEVVKLLLEADAAIVMLPDKSG 978
            R GHV+ +KALL KDPQLARRTDKKGQTALHMAVKG S EVVKLLL+ADAAIVMLPDK G
Sbjct: 238  RQGHVDIVKALLSKDPQLARRTDKKGQTALHMAVKGQSCEVVKLLLDADAAIVMLPDKFG 297

Query: 977  LTALHVATRKKRAEIVNELLSLPDTNVNALTRDHKTALDIAEGLSLSEESADIRACLVDR 798
             TALHVATRKKRAEIVNELL LPDTNVNALTRDHKTALDIAE L LSEES+DI+ CL   
Sbjct: 298  NTALHVATRKKRAEIVNELLLLPDTNVNALTRDHKTALDIAEELVLSEESSDIKECLYRY 357

Query: 797  GAVRANELNQPRDELRKTVTQIKNDVHIQLLQTKRTNKNVHGIAKELRKLHREGIXXXXX 618
            GA+RANELNQPRDELRKTVTQIKNDVH QL QT+RTNKNVH I+KELRKLHREGI     
Sbjct: 358  GALRANELNQPRDELRKTVTQIKNDVHTQLEQTRRTNKNVHNISKELRKLHREGINNATN 417

Query: 617  XXXXXXXXXXXXXXXXXXXVPGGDNDDGMAVVVNRTSFKIFFIFNAIALFTSLAVVVVQI 438
                               VPGGD + GMAVVV+ TSFKIFFIFNAIALFTSLAVVVVQI
Sbjct: 418  SVTVVAVLFATVAFAAIFTVPGGDLNSGMAVVVSHTSFKIFFIFNAIALFTSLAVVVVQI 477

Query: 437  TLVRGETKAERKVVEVINKLMWLASVCTSVAFMASSYIVVGRKYEWAAXXXXXXXXXXXX 258
            TLVRGETKAER+VVEVINKLMWLASVCTSVAFMAS+YIVVGR +EWAA            
Sbjct: 478  TLVRGETKAERRVVEVINKLMWLASVCTSVAFMASAYIVVGRTHEWAAVLITIVGGVIMT 537

Query: 257  XXXXXXTYYVVKSKKTRSMRRREKIA-RSGTNS-WMASEFSNSDVERIYAI 111
                  TYYVVKSK+ RSMR+R+K + RSG+NS    SEFSNS+V+RIYA+
Sbjct: 538  AVLGTMTYYVVKSKRIRSMRKRDKHSRRSGSNSGHHNSEFSNSEVDRIYAL 588


>ref|XP_002526791.1| ankyrin repeat-containing protein, putative [Ricinus communis]
            gi|223533867|gb|EEF35597.1| ankyrin repeat-containing
            protein, putative [Ricinus communis]
          Length = 570

 Score =  782 bits (2020), Expect = 0.0
 Identities = 423/586 (72%), Positives = 470/586 (80%), Gaps = 1/586 (0%)
 Frame = -2

Query: 1865 MGSAIDEGDLEKGLMTPSKQKQNSNPLREXXXXXXXXXXXXXALVLTNSGKRIDQAGKKK 1686
            M S I +  L +   TPS    +S P                 LVL+NSGKRIDQAGKKK
Sbjct: 1    MPSQIVQNPLAEPSPTPSPSSTSSAPA----------------LVLSNSGKRIDQAGKKK 44

Query: 1685 YVKQVTGRHNDTELHLAAQRGDLAAVKQILDDINSQMVGTLSGADFDAEVAEIRASVVNE 1506
            YVKQVTGRHNDTELHLAAQ+GDLAAVKQIL+DINSQ+VGTLSG +FDAEV EIRASVVNE
Sbjct: 45   YVKQVTGRHNDTELHLAAQQGDLAAVKQILNDINSQLVGTLSGEEFDAEVVEIRASVVNE 104

Query: 1505 VNELGETALYTAAERGHLEVVKELLKYSDKETISRRSRLEFDPLHIAASQGHHAVVQLLL 1326
            VNELGETAL+TAA++GHLEVVKELLKYS KE I+R++R  FD LHIAA QGHH +VQ+LL
Sbjct: 105  VNELGETALFTAADKGHLEVVKELLKYSSKECITRKNRSNFDALHIAAMQGHHGIVQVLL 164

Query: 1325 DHDVSLCQTRSQGNATPLITAASKGHTAVVHELLSKDPSLLDIPRSNGKNALHLAARSGH 1146
            DHD SL +T    NATPL++AA++GHTAVV+ELLSKD SLL+I RSNGKNALHLAAR GH
Sbjct: 165  DHDPSLSRTYGPSNATPLVSAATRGHTAVVNELLSKDGSLLEISRSNGKNALHLAARQGH 224

Query: 1145 VETLKALLDKDPQLARRTDKKGQTALHMAVKGASSEVVKLLLEADAAIVMLPDKSGLTAL 966
            V+ +KALL KDPQLARRTDKKGQTALHMAVKG S EVVKLLLEADAAIVMLPDK G TAL
Sbjct: 225  VDVVKALLSKDPQLARRTDKKGQTALHMAVKGQSCEVVKLLLEADAAIVMLPDKFGYTAL 284

Query: 965  HVATRKKRAEIVNELLSLPDTNVNALTRDHKTALDIAEGLSLSEESADIRACLVDRGAVR 786
            HVATRKKR EIVNELL LPDTNVNALTR+HKTALDIAE L+LSEES++I+ CL   GAVR
Sbjct: 285  HVATRKKRVEIVNELLLLPDTNVNALTREHKTALDIAEELTLSEESSEIKECLCRYGAVR 344

Query: 785  ANELNQPRDELRKTVTQIKNDVHIQLLQTKRTNKNVHGIAKELRKLHREGIXXXXXXXXX 606
            ANELNQPRDELRKTVTQIK DVH QL QT++TNKNVH I+KELRKLHREGI         
Sbjct: 345  ANELNQPRDELRKTVTQIKKDVHTQLEQTRKTNKNVHNISKELRKLHREGINNATNSVTV 404

Query: 605  XXXXXXXXXXXXXXXVPGGDNDDGMAVVVNRTSFKIFFIFNAIALFTSLAVVVVQITLVR 426
                           VPGGD+++G+AVVV   SFKIFFIFNAIALFTSLAVVVVQITLVR
Sbjct: 405  VAVLFATVAFAAIFTVPGGDDNNGIAVVVGHASFKIFFIFNAIALFTSLAVVVVQITLVR 464

Query: 425  GETKAERKVVEVINKLMWLASVCTSVAFMASSYIVVGRKYEWAAXXXXXXXXXXXXXXXX 246
            GETKAER+VVEVINKLMWLASVCTSVAF+ASSYIVVGRK+EWAA                
Sbjct: 465  GETKAERRVVEVINKLMWLASVCTSVAFIASSYIVVGRKHEWAAVLVTVVGGVIMAGVLG 524

Query: 245  XXTYYVVKSKKTRSMRRREKIA-RSGTNSWMASEFSNSDVERIYAI 111
              TYYVVKSK+ RSMR+REK A RSG+NSW  S+FSNS+V+RIYA+
Sbjct: 525  TMTYYVVKSKRIRSMRKREKSARRSGSNSWQHSDFSNSEVDRIYAL 570


>ref|NP_187842.1| ankyrin repeats-containing protein [Arabidopsis thaliana]
            gi|75308849|sp|Q9C7A2.1|Y3236_ARATH RecName: Full=Ankyrin
            repeat-containing protein At3g12360
            gi|12321945|gb|AAG51002.1|AC069474_1 ankyrin-like
            protein; 93648-91299 [Arabidopsis thaliana]
            gi|332641667|gb|AEE75188.1| ankyrin repeats-containing
            protein [Arabidopsis thaliana]
          Length = 590

 Score =  764 bits (1972), Expect = 0.0
 Identities = 411/581 (70%), Positives = 463/581 (79%), Gaps = 2/581 (0%)
 Frame = -2

Query: 1847 EGDLEKGLMTPSKQKQNSNPLREXXXXXXXXXXXXXA-LVLTNSGKRIDQAGKKKYVKQV 1671
            E D+EKG M   +  +N NP+ +               LVL+NSGKR+DQAGKKKYVKQV
Sbjct: 10   ERDMEKGGMILLQSSENQNPMIDPSPTPSPSATATAPALVLSNSGKRMDQAGKKKYVKQV 69

Query: 1670 TGRHNDTELHLAAQRGDLAAVKQILDDINSQMVGTLSGADFDAEVAEIRASVVNEVNELG 1491
            TGRHNDTELHLAAQRGDLAAV+QIL DINSQM G LSG +FDAEVAEIRAS+VNEVNELG
Sbjct: 70   TGRHNDTELHLAAQRGDLAAVQQILKDINSQMEGILSGEEFDAEVAEIRASIVNEVNELG 129

Query: 1490 ETALYTAAERGHLEVVKELLKYSDKETISRRSRLEFDPLHIAASQGHHAVVQLLLDHDVS 1311
            ETAL+TAA++GHL+VVKELLKYS +E+I++++R  +DPLHIAA QGHHA+V++LLDHD +
Sbjct: 130  ETALFTAADKGHLDVVKELLKYSSRESIAKKNRSGYDPLHIAAIQGHHAIVEVLLDHDAT 189

Query: 1310 LCQTRSQGNATPLITAASKGHTAVVHELLSKDPSLLDIPRSNGKNALHLAARSGHVETLK 1131
            L QT    NATPL++AA +GHT VV++LLSK  +LL+I RSN KNALHLAAR GHVE +K
Sbjct: 190  LSQTFGPSNATPLVSAAMRGHTEVVNQLLSKAGNLLEISRSNNKNALHLAARQGHVEVIK 249

Query: 1130 ALLDKDPQLARRTDKKGQTALHMAVKGASSEVVKLLLEADAAIVMLPDKSGLTALHVATR 951
            ALL KDPQLARR DKKGQTALHMAVKG SSEVVKLLL+AD AIVM PDKS  TALHVATR
Sbjct: 250  ALLSKDPQLARRIDKKGQTALHMAVKGQSSEVVKLLLDADPAIVMQPDKSCNTALHVATR 309

Query: 950  KKRAEIVNELLSLPDTNVNALTRDHKTALDIAEGLSLSEESADIRACLVDRGAVRANELN 771
            KKRAEIV  LLSLPDTN N LTRDHKTALDIAEGL LSEES+ I+ CL   GA+RANELN
Sbjct: 310  KKRAEIVELLLSLPDTNANTLTRDHKTALDIAEGLPLSEESSYIKECLARSGALRANELN 369

Query: 770  QPRDELRKTVTQIKNDVHIQLLQTKRTNKNVHGIAKELRKLHREGIXXXXXXXXXXXXXX 591
            QPRDELR TVTQIKNDVHIQL QTKRTNKNVH I+KELRKLHREGI              
Sbjct: 370  QPRDELRSTVTQIKNDVHIQLEQTKRTNKNVHNISKELRKLHREGINNATNSVTVVAVLF 429

Query: 590  XXXXXXXXXXVPGGDNDDGMAVVVNRTSFKIFFIFNAIALFTSLAVVVVQITLVRGETKA 411
                      VPGGDN+DG AVVV R SFKIFFIFNA+ALFTSLAVVVVQITLVRGETKA
Sbjct: 430  ATVAFAAIFTVPGGDNNDGSAVVVGRASFKIFFIFNALALFTSLAVVVVQITLVRGETKA 489

Query: 410  ERKVVEVINKLMWLASVCTSVAFMASSYIVVGRKYEWAAXXXXXXXXXXXXXXXXXXTYY 231
            E++VVEVINKLMWLAS+CTSVAF+ASSYIVVGRK EWAA                  TYY
Sbjct: 490  EKRVVEVINKLMWLASMCTSVAFLASSYIVVGRKNEWAAELVTVVGGVIMAGVLGTMTYY 549

Query: 230  VVKSKKTRSMRRREKIA-RSGTNSWMASEFSNSDVERIYAI 111
            VVKSK+TRSMR++ K A RSG+NSW  S+FSNS+V+ I+AI
Sbjct: 550  VVKSKRTRSMRKKVKSARRSGSNSWHHSDFSNSEVDPIFAI 590


>ref|XP_004149816.1| PREDICTED: ankyrin repeat-containing protein At3g12360-like [Cucumis
            sativus] gi|449518239|ref|XP_004166150.1| PREDICTED:
            ankyrin repeat-containing protein At3g12360-like [Cucumis
            sativus]
          Length = 590

 Score =  759 bits (1960), Expect = 0.0
 Identities = 412/580 (71%), Positives = 456/580 (78%), Gaps = 2/580 (0%)
 Frame = -2

Query: 1844 GDLEKGLMTPSKQKQNSNPLREXXXXXXXXXXXXXA-LVLTNSGKRIDQAGKKKYVKQVT 1668
            GDLEKG+++ S    N NPL E               LVL+NSGKR+DQAGKKKYVKQVT
Sbjct: 14   GDLEKGILSLSS---NQNPLAELSPSPSPSSTATAPALVLSNSGKRMDQAGKKKYVKQVT 70

Query: 1667 GRHNDTELHLAAQRGDLAAVKQILDDINSQMVGTLSGADFDAEVAEIRASVVNEVNELGE 1488
            GRHNDTELHLAAQRGDLAAVKQILDDI+SQMV  LSGADFDAEVAE+R+ VVNEVNELGE
Sbjct: 71   GRHNDTELHLAAQRGDLAAVKQILDDIDSQMVRNLSGADFDAEVAEVRSLVVNEVNELGE 130

Query: 1487 TALYTAAERGHLEVVKELLKYSDKETISRRSRLEFDPLHIAASQGHHAVVQLLLDHDVSL 1308
            TAL+TAAERGH+EVVKELLKYS+KET++ ++R  FDPLHIAASQGHHA+VQ+LL+H+ SL
Sbjct: 131  TALFTAAERGHIEVVKELLKYSNKETLTTKNRSAFDPLHIAASQGHHAIVQVLLEHEPSL 190

Query: 1307 CQTRSQGNATPLITAASKGHTAVVHELLSKDPSLLDIPRSNGKNALHLAARSGHVETLKA 1128
             QT    NATPLITAA++GHTAVV ELL+KD +LL+I RSNGKNALH A R GH E +K 
Sbjct: 191  SQTFGPSNATPLITAAARGHTAVVEELLNKDRNLLEICRSNGKNALHFAVRPGHTEIVKL 250

Query: 1127 LLDKDPQLARRTDKKGQTALHMAVKGASSEVVKLLLEADAAIVMLPDKSGLTALHVATRK 948
            LL KDP LAR+ DKKGQTALHMAVKG S +VVKLLLEAD AIVMLPDK G TALHVATRK
Sbjct: 251  LLSKDPHLARKNDKKGQTALHMAVKGQSRDVVKLLLEADPAIVMLPDKFGNTALHVATRK 310

Query: 947  KRAEIVNELLSLPDTNVNALTRDHKTALDIAEGLSLSEESADIRACLVDRGAVRANELNQ 768
            KR EIV ELL LPDTNVNAL+RDHKTA DIAE L LSEES++I+  L   GAVRANELNQ
Sbjct: 311  KRVEIVQELLLLPDTNVNALSRDHKTAFDIAEELPLSEESSEIKDSLSRYGAVRANELNQ 370

Query: 767  PRDELRKTVTQIKNDVHIQLLQTKRTNKNVHGIAKELRKLHREGIXXXXXXXXXXXXXXX 588
            PRDELR TVTQIK DVH QL QT++TNKNVH I+KELRKLHREGI               
Sbjct: 371  PRDELRNTVTQIKKDVHTQLEQTRKTNKNVHNISKELRKLHREGINNATNSVTVVAVLFA 430

Query: 587  XXXXXXXXXVPGGDNDDGMAVVVNRTSFKIFFIFNAIALFTSLAVVVVQITLVRGETKAE 408
                     VPGGD D G AVVV   SFKIFFIFNAIALFTSLAVVVVQITLVRGETKAE
Sbjct: 431  TVAFAAIFTVPGGDTDQGTAVVVGTISFKIFFIFNAIALFTSLAVVVVQITLVRGETKAE 490

Query: 407  RKVVEVINKLMWLASVCTSVAFMASSYIVVGRKYEWAAXXXXXXXXXXXXXXXXXXTYYV 228
            R+VVE+INKLMWLASVCTSVAFMASSYIVVG KY WAA                  TYYV
Sbjct: 491  RRVVEIINKLMWLASVCTSVAFMASSYIVVGHKYRWAAAVITVVGGVIMAGVLGTMTYYV 550

Query: 227  VKSKKTRSMRRREKI-ARSGTNSWMASEFSNSDVERIYAI 111
            VKSK  RS+R++EK   RSG+NSW  S+FSNS+V+RIYA+
Sbjct: 551  VKSKSRRSVRKKEKSNRRSGSNSWHHSDFSNSEVDRIYAL 590


>ref|XP_002282204.1| PREDICTED: ankyrin repeat-containing protein At3g12360 [Vitis
            vinifera] gi|297736432|emb|CBI25303.3| unnamed protein
            product [Vitis vinifera]
          Length = 589

 Score =  757 bits (1955), Expect = 0.0
 Identities = 407/581 (70%), Positives = 464/581 (79%), Gaps = 2/581 (0%)
 Frame = -2

Query: 1847 EGDLEKGLMTPSKQKQNSNPLREXXXXXXXXXXXXXA-LVLTNSGKRIDQAGKKKYVKQV 1671
            E DLEKGL+TP+    N NPL E               LVL++S KRIDQAGKKKYVKQV
Sbjct: 12   ERDLEKGLVTPAP---NQNPLAEPSPSPSPSSTASAPALVLSSSSKRIDQAGKKKYVKQV 68

Query: 1670 TGRHNDTELHLAAQRGDLAAVKQILDDINSQMVGTLSGADFDAEVAEIRASVVNEVNELG 1491
            TGRHNDTELHLAAQRGDLAAVKQILDDI+SQ+  T+SGA+F+AEVAE+R  +VNEVNELG
Sbjct: 69   TGRHNDTELHLAAQRGDLAAVKQILDDIHSQIGDTMSGAEFEAEVAEVRTLMVNEVNELG 128

Query: 1490 ETALYTAAERGHLEVVKELLKYSDKETISRRSRLEFDPLHIAASQGHHAVVQLLLDHDVS 1311
            ETAL+TAAE+GH++VVKELLKYS++E+ISR++R +F PLHIAA+QGHHA+VQ+LLD+D  
Sbjct: 129  ETALFTAAEKGHIDVVKELLKYSNRESISRKNRSQFGPLHIAAAQGHHAIVQVLLDYDPE 188

Query: 1310 LCQTRSQGNATPLITAASKGHTAVVHELLSKDPSLLDIPRSNGKNALHLAARSGHVETLK 1131
            L +T    NATPL++AAS+GHTAVV ELLSKD  LL+I +SNGKNALHLAAR GHV+ ++
Sbjct: 189  LSKTIGPSNATPLVSAASRGHTAVVIELLSKDCGLLEIAKSNGKNALHLAARQGHVDIVE 248

Query: 1130 ALLDKDPQLARRTDKKGQTALHMAVKGASSEVVKLLLEADAAIVMLPDKSGLTALHVATR 951
            ALL+KDPQLARRTDKKGQTALHMAVKG S EVVKLLL ADAAIVMLPDK G TALHVATR
Sbjct: 249  ALLEKDPQLARRTDKKGQTALHMAVKGVSCEVVKLLLNADAAIVMLPDKQGNTALHVATR 308

Query: 950  KKRAEIVNELLSLPDTNVNALTRDHKTALDIAEGLSLSEESADIRACLVDRGAVRANELN 771
            KKRAEIVNELL LPD NVNAL RDHKT+LDIAE LS SEE+++I+ CL+  GA++ANELN
Sbjct: 309  KKRAEIVNELLRLPDANVNALNRDHKTSLDIAEDLSHSEEASEIKDCLIRYGAIKANELN 368

Query: 770  QPRDELRKTVTQIKNDVHIQLLQTKRTNKNVHGIAKELRKLHREGIXXXXXXXXXXXXXX 591
            QPRDELR TVTQIK DVH QL QT+RTNKNVH I+KELRKLHREGI              
Sbjct: 369  QPRDELRNTVTQIKRDVHTQLEQTRRTNKNVHNISKELRKLHREGINNATNSVTVVAVLF 428

Query: 590  XXXXXXXXXXVPGGDNDDGMAVVVNRTSFKIFFIFNAIALFTSLAVVVVQITLVRGETKA 411
                      VPGGDN DG  VVV   SFKIFFIFNA+ALFTSLAVVVVQITLVRGETKA
Sbjct: 429  ATVAFAAIFTVPGGDNPDGTGVVVKSASFKIFFIFNALALFTSLAVVVVQITLVRGETKA 488

Query: 410  ERKVVEVINKLMWLASVCTSVAFMASSYIVVGRKYEWAAXXXXXXXXXXXXXXXXXXTYY 231
            ER VV VINKLMWLASVCTSVAF+ASSYIVVGR  EWAA                  TYY
Sbjct: 489  ERNVVVVINKLMWLASVCTSVAFIASSYIVVGRHNEWAAIFVTVVGGVIMAGVLGTMTYY 548

Query: 230  VVKSKKTRSMRRREKIA-RSGTNSWMASEFSNSDVERIYAI 111
            VVKSK+ RS+RR++K A RSG+NSW  SEFS+S+++RIYA+
Sbjct: 549  VVKSKRIRSVRRKDKNARRSGSNSWHHSEFSDSEIDRIYAL 589