BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes22_contig00006464
         (1206 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AFO64617.1| cytochrome P450 mono-oxygenase [Artemisia annua]       511   e-142
emb|CAA04116.1| cytochrome P450 [Helianthus tuberosus]                492   e-137
emb|CAA04117.1| cytochrome P450 [Helianthus tuberosus]                492   e-137
dbj|BAF98467.1| cytochrome P450 [Coptis japonica var. dissecta]       394   e-107
gb|AGC29952.1| CYP81B57 [Sinopodophyllum hexandrum]                   391   e-106

>gb|AFO64617.1| cytochrome P450 mono-oxygenase [Artemisia annua]
          Length = 502

 Score =  511 bits (1315), Expect = e-142
 Identities = 246/287 (85%), Positives = 265/287 (92%)
 Frame = -2

Query: 863  SGKRYFGGDIPEVEEEGKRFRQILDETFVLAGAANVSDYLPILSWLGVKGLEKKLIALQG 684
            SGKRYFGGDIPEVE EGKRFR+ILDETF+LAGAANV DYLP L W GV  LEKKL+AL+ 
Sbjct: 184  SGKRYFGGDIPEVEAEGKRFREILDETFLLAGAANVGDYLPFLRWFGVTKLEKKLVALRE 243

Query: 683  KRDVFFQGLIEQLRKAKRGETENKKKTMIEVLLSLQESDPEYYTDQMIRSFVLVLLSAGT 504
            KR+VFFQGLIEQLRK K  E  N KKTMIEVLLSLQESDP+YYTD+MIRSFVLVLLSAGT
Sbjct: 244  KREVFFQGLIEQLRKPKGAEVGNNKKTMIEVLLSLQESDPKYYTDEMIRSFVLVLLSAGT 303

Query: 503  DTSAGTMQWAMSLLLNNPQVIKKAQNEIDKCVGTDRLVDESDIANLPYLRCIINETLRLY 324
            DTSAGTM+WAMSLLLN+PQV+KKAQNEID+ VG DRLVDESD+ NLPYLRCIINETLR+ 
Sbjct: 304  DTSAGTMEWAMSLLLNHPQVLKKAQNEIDRVVGNDRLVDESDVVNLPYLRCIINETLRIC 363

Query: 323  PAGPLLVPHESSEECVIGGYNIPRGTMLLVNQWAVHHDPKLWTEPERFNPERFVGVEGTR 144
            P GPLLVPHESSE+CVIGGYNIPRGTMLLVNQWA+HHDPKLWT+PE F PERF G+EGTR
Sbjct: 364  PPGPLLVPHESSEDCVIGGYNIPRGTMLLVNQWAIHHDPKLWTDPEMFKPERFEGLEGTR 423

Query: 143  DGFKLMPFGSGRRSCPGEGLAVRVLGSTLGLLIQCFDWERMSEKMVD 3
            DGFKLMPFGSGRRSCPGEGLAVRV+GSTLGLLIQCFDWER+SEKMVD
Sbjct: 424  DGFKLMPFGSGRRSCPGEGLAVRVIGSTLGLLIQCFDWERLSEKMVD 470



 Score =  134 bits (336), Expect = 6e-29
 Identities = 62/71 (87%), Positives = 69/71 (97%)
 Frame = -1

Query: 1206 IIFANRPHMLFGKIIGNNYTSLAWAPYGDNWRNLRRIASIEILSIHRLNEFHDVRVEEGR 1027
            IIFANRP MLFGKIIGNNYTSLAW+PYGDNWRNLRRIASIEILSIHRLNEFHD+RV+EG+
Sbjct: 92   IIFANRPRMLFGKIIGNNYTSLAWSPYGDNWRNLRRIASIEILSIHRLNEFHDIRVDEGK 151

Query: 1026 HLIRKLLSHSS 994
             L+RKL+S+SS
Sbjct: 152  LLVRKLVSNSS 162


>emb|CAA04116.1| cytochrome P450 [Helianthus tuberosus]
          Length = 505

 Score =  492 bits (1267), Expect = e-137
 Identities = 233/287 (81%), Positives = 263/287 (91%)
 Frame = -2

Query: 863  SGKRYFGGDIPEVEEEGKRFRQILDETFVLAGAANVSDYLPILSWLGVKGLEKKLIALQG 684
            SGKRYFGGD PE+EEEGKRFR +LDETFVLAGA+NV DYLP+LSWLGVKGLEKKLI LQ 
Sbjct: 187  SGKRYFGGDNPELEEEGKRFRDMLDETFVLAGASNVGDYLPVLSWLGVKGLEKKLIKLQE 246

Query: 683  KRDVFFQGLIEQLRKAKRGETENKKKTMIEVLLSLQESDPEYYTDQMIRSFVLVLLSAGT 504
            KRDVFFQGLI+QLRK+K  E  NKKKTMIE+LLSLQE++PEYYTD MIRSFVLVLL+AG+
Sbjct: 247  KRDVFFQGLIDQLRKSKGTEDVNKKKTMIELLLSLQETEPEYYTDAMIRSFVLVLLAAGS 306

Query: 503  DTSAGTMQWAMSLLLNNPQVIKKAQNEIDKCVGTDRLVDESDIANLPYLRCIINETLRLY 324
            DTSAGTM+W MSLLLN+PQV+KKAQNEID  +G + LVDESDI NLPYLRCIINETLRLY
Sbjct: 307  DTSAGTMEWVMSLLLNHPQVLKKAQNEIDSVIGKNCLVDESDIPNLPYLRCIINETLRLY 366

Query: 323  PAGPLLVPHESSEECVIGGYNIPRGTMLLVNQWAVHHDPKLWTEPERFNPERFVGVEGTR 144
            PAGPLLVPHE+S +CV+GGYN+PRGT+L+VNQWA+HHDPK+W EPE F PERF G+EGTR
Sbjct: 367  PAGPLLVPHEASSDCVVGGYNVPRGTILIVNQWAIHHDPKVWDEPETFKPERFEGLEGTR 426

Query: 143  DGFKLMPFGSGRRSCPGEGLAVRVLGSTLGLLIQCFDWERMSEKMVD 3
            DGFKL+PFGSGRRSCPGEGLAVR+LG TLG +IQCFDWER SE++VD
Sbjct: 427  DGFKLLPFGSGRRSCPGEGLAVRMLGMTLGSIIQCFDWERTSEELVD 473



 Score =  125 bits (314), Expect = 2e-26
 Identities = 60/68 (88%), Positives = 64/68 (94%)
 Frame = -1

Query: 1206 IIFANRPHMLFGKIIGNNYTSLAWAPYGDNWRNLRRIASIEILSIHRLNEFHDVRVEEGR 1027
            I+FANRP MLFGKIIG NYTSLAW+PYGDNWRNLRRIASIEILSIHRLNEFHD+RVEE R
Sbjct: 92   IVFANRPKMLFGKIIGVNYTSLAWSPYGDNWRNLRRIASIEILSIHRLNEFHDIRVEETR 151

Query: 1026 HLIRKLLS 1003
             LI+KLLS
Sbjct: 152  LLIQKLLS 159


>emb|CAA04117.1| cytochrome P450 [Helianthus tuberosus]
          Length = 520

 Score =  492 bits (1267), Expect = e-137
 Identities = 233/287 (81%), Positives = 263/287 (91%)
 Frame = -2

Query: 863  SGKRYFGGDIPEVEEEGKRFRQILDETFVLAGAANVSDYLPILSWLGVKGLEKKLIALQG 684
            SGKRYFGGD PE+EEEGKRFR +LDETFVLAGA+NV DYLP+LSWLGVKGLEKKLI LQ 
Sbjct: 202  SGKRYFGGDNPELEEEGKRFRDMLDETFVLAGASNVGDYLPVLSWLGVKGLEKKLIKLQE 261

Query: 683  KRDVFFQGLIEQLRKAKRGETENKKKTMIEVLLSLQESDPEYYTDQMIRSFVLVLLSAGT 504
            KRDVFFQGLI+QLRK+K  E  NKKKTMIE+LLSLQE++PEYYTD MIRSFVLVLL+AG+
Sbjct: 262  KRDVFFQGLIDQLRKSKGTEDVNKKKTMIELLLSLQETEPEYYTDAMIRSFVLVLLAAGS 321

Query: 503  DTSAGTMQWAMSLLLNNPQVIKKAQNEIDKCVGTDRLVDESDIANLPYLRCIINETLRLY 324
            DTSAGTM+W MSLLLN+PQV+KKAQNEID  +G + LVDESDI NLPYLRCIINETLRLY
Sbjct: 322  DTSAGTMEWVMSLLLNHPQVLKKAQNEIDSVIGKNCLVDESDIPNLPYLRCIINETLRLY 381

Query: 323  PAGPLLVPHESSEECVIGGYNIPRGTMLLVNQWAVHHDPKLWTEPERFNPERFVGVEGTR 144
            PAGPLLVPHE+S +CV+GGYN+PRGT+L+VNQWA+HHDPK+W EPE F PERF G+EGTR
Sbjct: 382  PAGPLLVPHEASSDCVVGGYNVPRGTILIVNQWAIHHDPKVWDEPETFKPERFEGLEGTR 441

Query: 143  DGFKLMPFGSGRRSCPGEGLAVRVLGSTLGLLIQCFDWERMSEKMVD 3
            DGFKL+PFGSGRRSCPGEGLAVR+LG TLG +IQCFDWER SE++VD
Sbjct: 442  DGFKLLPFGSGRRSCPGEGLAVRMLGMTLGSIIQCFDWERTSEELVD 488



 Score =  125 bits (314), Expect = 2e-26
 Identities = 60/68 (88%), Positives = 64/68 (94%)
 Frame = -1

Query: 1206 IIFANRPHMLFGKIIGNNYTSLAWAPYGDNWRNLRRIASIEILSIHRLNEFHDVRVEEGR 1027
            I+FANRP MLFGKIIG NYTSLAW+PYGDNWRNLRRIASIEILSIHRLNEFHD+RVEE R
Sbjct: 107  IVFANRPKMLFGKIIGVNYTSLAWSPYGDNWRNLRRIASIEILSIHRLNEFHDIRVEETR 166

Query: 1026 HLIRKLLS 1003
             LI+KLLS
Sbjct: 167  LLIQKLLS 174


>dbj|BAF98467.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 503

 Score =  394 bits (1011), Expect = e-107
 Identities = 187/289 (64%), Positives = 233/289 (80%), Gaps = 2/289 (0%)
 Frame = -2

Query: 863  SGKRYFGGDIPEVEEEGKRFRQILDETFVLAGAANVSDYLPILSWLGVKGLEKKLIALQG 684
            +GKRY+GG + E+E+  KRF++I+  T  ++GA+N+ D+LPILSW+G+KG+EKK+  L+ 
Sbjct: 184  AGKRYYGGQVEELEQ-AKRFQEIVQGTLAISGASNIGDFLPILSWIGIKGIEKKVWNLKE 242

Query: 683  KRDVFFQGLIEQLRKAKRG--ETENKKKTMIEVLLSLQESDPEYYTDQMIRSFVLVLLSA 510
            KRD F Q LIE+ R ++ G  +T+ K  T+I+VLLSLQE++PEYYTD+MI   + VLL+A
Sbjct: 243  KRDKFLQELIEEHRISRSGGMQTKEKNATLIDVLLSLQETEPEYYTDEMILGIIWVLLAA 302

Query: 509  GTDTSAGTMQWAMSLLLNNPQVIKKAQNEIDKCVGTDRLVDESDIANLPYLRCIINETLR 330
            GTDTSA TM+WAMSLLLNNPQVIKKAQ EID  +   RL++ESD+  LPYL  II ETLR
Sbjct: 303  GTDTSAVTMEWAMSLLLNNPQVIKKAQAEIDNNLEQGRLINESDVNKLPYLHSIITETLR 362

Query: 329  LYPAGPLLVPHESSEECVIGGYNIPRGTMLLVNQWAVHHDPKLWTEPERFNPERFVGVEG 150
            +YPAGPLLVPHESSEEC++GGY +P GTMLLVN WA+  DP +W EP +F PERF G EG
Sbjct: 363  IYPAGPLLVPHESSEECIVGGYKVPSGTMLLVNVWAIQQDPNIWVEPTKFKPERFDGFEG 422

Query: 149  TRDGFKLMPFGSGRRSCPGEGLAVRVLGSTLGLLIQCFDWERMSEKMVD 3
            TRDGFKLMPFGSGRR CPGEGLA+RV+   LG LIQCFDWER+ E+MVD
Sbjct: 423  TRDGFKLMPFGSGRRGCPGEGLAMRVVALALGALIQCFDWERVGEEMVD 471



 Score = 92.4 bits (228), Expect = 2e-16
 Identities = 38/66 (57%), Positives = 56/66 (84%)
 Frame = -1

Query: 1206 IIFANRPHMLFGKIIGNNYTSLAWAPYGDNWRNLRRIASIEILSIHRLNEFHDVRVEEGR 1027
            IIFANRPH+L GKI+G++YT+L WAPYG NWRNLR+I++IE+LS +RL  + D+R +E +
Sbjct: 90   IIFANRPHLLMGKILGHDYTTLLWAPYGPNWRNLRKISAIELLSANRLQSYSDIRHDEVK 149

Query: 1026 HLIRKL 1009
             ++++L
Sbjct: 150  SMVQRL 155


>gb|AGC29952.1| CYP81B57 [Sinopodophyllum hexandrum]
          Length = 507

 Score =  391 bits (1005), Expect = e-106
 Identities = 186/292 (63%), Positives = 236/292 (80%), Gaps = 5/292 (1%)
 Frame = -2

Query: 863  SGKRYFGGDIPEVEEEGKRFRQILDETFVLAGAANVSDYLPILSWLGVKGLEKKLIALQG 684
            +GKRY+G  + ++E+  ++F++I++ETF L+GA+NV D+LP+L W+G KG+EK+LI L+ 
Sbjct: 185  AGKRYYGEHVADLEQ-ARKFKEIVEETFELSGASNVGDFLPLLRWIGYKGIEKRLIRLKN 243

Query: 683  KRDVFFQGLIEQLRK----AKRGETENKKKTMIEVLLSLQESDPEYYTDQMIRSFVLVLL 516
            KRD F Q LI++ RK    +K   +E KKKT+I+VLLSLQE++PEYYTD+MIR  + +L 
Sbjct: 244  KRDEFLQELIQERRKLRVNSKSFPSEEKKKTLIDVLLSLQETEPEYYTDEMIRGLIWILF 303

Query: 515  SAGTDTSAGTMQWAMSLLLNNPQVIKKAQNEIDKCVGTDRLVDESDIANLPYLRCIINET 336
            +AGTDTSAGTM+WAMSLLLNNP +IKKAQ+EID  +   R +DESD+  LPYL CIINET
Sbjct: 304  AAGTDTSAGTMEWAMSLLLNNPDLIKKAQSEIDLQIEPGRPIDESDLNKLPYLHCIINET 363

Query: 335  LRLYPAGPLLVPHESSEECVIGGYNIPRGTMLLVNQWAVHHDPKLWTEPERFNPERFVGV 156
            LR+YPAGPLL+PHESSE+C IGGYNIP GTMLLVN WA+ +DP LW EP +F PERF G 
Sbjct: 364  LRMYPAGPLLIPHESSEDCEIGGYNIPCGTMLLVNLWAIQNDPNLWKEPRKFKPERFEGY 423

Query: 155  E-GTRDGFKLMPFGSGRRSCPGEGLAVRVLGSTLGLLIQCFDWERMSEKMVD 3
            + G RDGFKLMPFGSGRR CPGEGLA RV+G TL  L+QCFDW R+ E+MV+
Sbjct: 424  QGGVRDGFKLMPFGSGRRGCPGEGLAXRVVGLTLXSLLQCFDWHRVGEEMVE 475



 Score = 83.6 bits (205), Expect = 9e-14
 Identities = 34/66 (51%), Positives = 52/66 (78%)
 Frame = -1

Query: 1200 FANRPHMLFGKIIGNNYTSLAWAPYGDNWRNLRRIASIEILSIHRLNEFHDVRVEEGRHL 1021
            FANRP ++ GK  GNNYT+LAWA YG NWRNLRRI+++E+ S +R+  F ++R +E + +
Sbjct: 94   FANRPRLMIGKHSGNNYTTLAWASYGQNWRNLRRISALEMFSPNRIQMFSEIRTDEVKSM 153

Query: 1020 IRKLLS 1003
            +R+L++
Sbjct: 154  VRRLVA 159