BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes22_contig00006400
         (1533 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAG71197.1| ent-kaurene oxidase 1 [Lactuca sativa]                847   0.0  
gb|AAQ63464.1| ent-kaurene oxidase CYP701A5 [Stevia rebaudiana] ...   763   0.0  
gb|AAY42951.1| kaurene oxidase [Stevia rebaudiana]                    760   0.0  
dbj|BAG71198.1| ent-kaurene oxidase 2 [Lactuca sativa]                744   0.0  
gb|AFD54196.1| ent-kaurene oxidase [Vitis vinifera]                   645   0.0  

>dbj|BAG71197.1| ent-kaurene oxidase 1 [Lactuca sativa]
          Length = 511

 Score =  847 bits (2188), Expect = 0.0
 Identities = 410/485 (84%), Positives = 449/485 (92%)
 Frame = -2

Query: 1460 LSFLILKSFLSHQRRNPNHLPTVPAVPGVPLLGNLLQLKEKKPYKTFTKWAETYGPIYSI 1281
            +S L LKSFLS Q  NPNHLP+VPAVPGVPLLGNLL+LKEKKPYKTFTKWAETYGPIYSI
Sbjct: 27   ISLLFLKSFLSQQPGNPNHLPSVPAVPGVPLLGNLLELKEKKPYKTFTKWAETYGPIYSI 86

Query: 1280 KAGATSMVVVSSNHLAKEAMVTRFDSISTRKLSKALRILTADKTMVAMSDYDDYHKTVKR 1101
            K GATSMVVV+SN LAKEAMVTRFDSISTRKLSKAL+ILTADKTMVAMSDYDDYHKTVKR
Sbjct: 87   KTGATSMVVVNSNQLAKEAMVTRFDSISTRKLSKALQILTADKTMVAMSDYDDYHKTVKR 146

Query: 1100 NILTSVLGPAARKKHRAHRDTMADNVSNQLHALAPTSHREAINLREILQSELFTLAFKQV 921
            N+LTS+LGPAA+K+HRAHRD M DN+S QLHALA  S +EAIN R+I QSELFTLAFKQ 
Sbjct: 147  NLLTSILGPAAQKRHRAHRDAMGDNLSRQLHALALNSPQEAINFRQIFQSELFTLAFKQT 206

Query: 920  MGKDFESIYVGDLGITMTKEEVFQVLVVDPMMGAIDVDWRDFFPYLRWIPNTSFEKKIEQ 741
             G+D ESI+VGDLG TMT+EE+FQ+LVVDPMMGAIDVDWRDFFPYL+WIPN   E+KIEQ
Sbjct: 207  FGRDIESIFVGDLGTTMTREEMFQILVVDPMMGAIDVDWRDFFPYLKWIPNAKLEEKIEQ 266

Query: 740  MDIRREAVMKTLIQEHRKRIDSGETLNSYIDYLLSEAQPLTEKQLLMSLWEPIIETSDTT 561
            M IRR+AVMK +IQEHRKRIDSGE L+SYID+LL+EAQPLTEKQLLMSLWEPIIETSDTT
Sbjct: 267  MYIRRKAVMKAVIQEHRKRIDSGENLDSYIDFLLAEAQPLTEKQLLMSLWEPIIETSDTT 326

Query: 560  MVTTEWAMYELAKNPKKQDRLYDEIRNVCGSQKITEEKLCQMPYLSAIFHETLRRHSPVP 381
            MVTTEWAMYEL+K+P KQ RLY+EIRN+CGS+KITEEKLC+MPYLSA+FHETLR HSPV 
Sbjct: 327  MVTTEWAMYELSKHPNKQQRLYNEIRNICGSEKITEEKLCKMPYLSAVFHETLRVHSPVS 386

Query: 380  IIPLRYVHENTVLGGYHVPAGTELAINIYGCNMEREIWANPEEWNPERFLAENEPIDLQR 201
            IIPLRYVHENT LGGYHVPAGTELA+NIYGCNMEREIW NPEEW+PERFLAENEP++LQ+
Sbjct: 387  IIPLRYVHENTELGGYHVPAGTELAVNIYGCNMEREIWENPEEWSPERFLAENEPVNLQK 446

Query: 200  TMAFGGGKRVCAGAMQAMLIACVCMGRMVQEFEWRLKDDTGEDVNTIGLTTQMLNPMLAV 21
            TMAFG GKRVCAGAMQAML+ACV +GRMVQEFEWRLKDD  EDVNT+GLTTQ LNPMLAV
Sbjct: 447  TMAFGAGKRVCAGAMQAMLLACVGIGRMVQEFEWRLKDDVEEDVNTLGLTTQRLNPMLAV 506

Query: 20   IKPRN 6
            IKPRN
Sbjct: 507  IKPRN 511


>gb|AAQ63464.1| ent-kaurene oxidase CYP701A5 [Stevia rebaudiana]
            gi|76446107|gb|ABA42921.1| kaurene oxidase [Stevia
            rebaudiana]
          Length = 513

 Score =  763 bits (1969), Expect = 0.0
 Identities = 369/485 (76%), Positives = 424/485 (87%), Gaps = 1/485 (0%)
 Frame = -2

Query: 1460 LSFLILKSFLSHQRRNPNHLPTVPAVPGVPLLGNLLQLKEKKPYKTFTKWAETYGPIYSI 1281
            L F  LKS+ S +R   NHLP VP VPGVPLLGNLLQLKEKKPY TFT+WA TYGPIYSI
Sbjct: 28   LIFWYLKSYTSARRSQSNHLPRVPEVPGVPLLGNLLQLKEKKPYMTFTRWAATYGPIYSI 87

Query: 1280 KAGATSMVVVSSNHLAKEAMVTRFDSISTRKLSKALRILTADKTMVAMSDYDDYHKTVKR 1101
            K GATSMVVVSSN +AKEA+VTRF SISTR LSKAL++LTADKTMVAMSDYDDYHKTVKR
Sbjct: 88   KTGATSMVVVSSNEIAKEALVTRFQSISTRNLSKALKVLTADKTMVAMSDYDDYHKTVKR 147

Query: 1100 NILTSVLGPAARKKHRAHRDTMADNVSNQLHALAPTS-HREAINLREILQSELFTLAFKQ 924
            +ILT+VLGP A+KKHR HRD M DN+S QLH     +  +E ++LR+I QSELF LA +Q
Sbjct: 148  HILTAVLGPNAQKKHRIHRDIMMDNISTQLHEFVKNNPEQEEVDLRKIFQSELFGLAMRQ 207

Query: 923  VMGKDFESIYVGDLGITMTKEEVFQVLVVDPMMGAIDVDWRDFFPYLRWIPNTSFEKKIE 744
             +GKD ES+YV DL ITM ++E+FQVLVVDPMMGAIDVDWRDFFPYL+W+PN  FE  I+
Sbjct: 208  ALGKDVESLYVEDLKITMNRDEIFQVLVVDPMMGAIDVDWRDFFPYLKWVPNKKFENTIQ 267

Query: 743  QMDIRREAVMKTLIQEHRKRIDSGETLNSYIDYLLSEAQPLTEKQLLMSLWEPIIETSDT 564
            QM IRREAVMK+LI+EH+KRI SGE LNSYIDYLLSEAQ LT++QLLMSLWEPIIE+SDT
Sbjct: 268  QMYIRREAVMKSLIKEHKKRIASGEKLNSYIDYLLSEAQTLTDQQLLMSLWEPIIESSDT 327

Query: 563  TMVTTEWAMYELAKNPKKQDRLYDEIRNVCGSQKITEEKLCQMPYLSAIFHETLRRHSPV 384
            TMVTTEWAMYELAKNPK QDRLY +I++VCGS+KITEE L Q+PY++AIFHETLRRHSPV
Sbjct: 328  TMVTTEWAMYELAKNPKLQDRLYRDIKSVCGSEKITEEHLSQLPYITAIFHETLRRHSPV 387

Query: 383  PIIPLRYVHENTVLGGYHVPAGTELAINIYGCNMEREIWANPEEWNPERFLAENEPIDLQ 204
            PIIPLR+VHE+TVLGGYHVPAGTELA+NIYGCNM++ +W NPEEWNPERF+ ENE ID Q
Sbjct: 388  PIIPLRHVHEDTVLGGYHVPAGTELAVNIYGCNMDKNVWENPEEWNPERFMKENETIDFQ 447

Query: 203  RTMAFGGGKRVCAGAMQAMLIACVCMGRMVQEFEWRLKDDTGEDVNTIGLTTQMLNPMLA 24
            +TMAFGGGKRVCAG++QA+L A + +GRMVQEFEW+LKD T E+VNTIGLTTQML P+ A
Sbjct: 448  KTMAFGGGKRVCAGSLQALLTASIGIGRMVQEFEWKLKDMTQEEVNTIGLTTQMLRPLRA 507

Query: 23   VIKPR 9
            +IKPR
Sbjct: 508  IIKPR 512


>gb|AAY42951.1| kaurene oxidase [Stevia rebaudiana]
          Length = 513

 Score =  760 bits (1962), Expect = 0.0
 Identities = 368/485 (75%), Positives = 424/485 (87%), Gaps = 1/485 (0%)
 Frame = -2

Query: 1460 LSFLILKSFLSHQRRNPNHLPTVPAVPGVPLLGNLLQLKEKKPYKTFTKWAETYGPIYSI 1281
            L F  LKS+ S +R   NHLP VP VPGVPLLGNLLQLKEKKPY TFT+WA TYGPIYSI
Sbjct: 28   LIFWYLKSYTSARRSQSNHLPRVPEVPGVPLLGNLLQLKEKKPYMTFTRWAATYGPIYSI 87

Query: 1280 KAGATSMVVVSSNHLAKEAMVTRFDSISTRKLSKALRILTADKTMVAMSDYDDYHKTVKR 1101
            K GATSMVVVSSN +AKEA+VTRF SISTR LSKAL++LTADKTMVAMSDYDDYHKTVKR
Sbjct: 88   KTGATSMVVVSSNEIAKEALVTRFQSISTRNLSKALKVLTADKTMVAMSDYDDYHKTVKR 147

Query: 1100 NILTSVLGPAARKKHRAHRDTMADNVSNQLHALAPTS-HREAINLREILQSELFTLAFKQ 924
            +ILT+VLGP A+KKHR HRD M DN+S QLH     +  +E ++LR+I QSELF LA +Q
Sbjct: 148  HILTAVLGPNAQKKHRIHRDIMMDNISTQLHEFVKNNPEQEEVDLRKIFQSELFGLAMRQ 207

Query: 923  VMGKDFESIYVGDLGITMTKEEVFQVLVVDPMMGAIDVDWRDFFPYLRWIPNTSFEKKIE 744
             +GKD ES+YV DL ITM ++E+FQVLVVDPMMGAIDVDWRDFFPYL+W+PN  FE  I+
Sbjct: 208  ALGKDVESLYVEDLKITMNRDEIFQVLVVDPMMGAIDVDWRDFFPYLKWVPNKKFENTIQ 267

Query: 743  QMDIRREAVMKTLIQEHRKRIDSGETLNSYIDYLLSEAQPLTEKQLLMSLWEPIIETSDT 564
            QM IRREAVMK+LI+E++KRI SGE LNSYIDYLLSEAQ LT++QLLMSLWEPIIE+SDT
Sbjct: 268  QMYIRREAVMKSLIKENKKRIASGEKLNSYIDYLLSEAQTLTDQQLLMSLWEPIIESSDT 327

Query: 563  TMVTTEWAMYELAKNPKKQDRLYDEIRNVCGSQKITEEKLCQMPYLSAIFHETLRRHSPV 384
            TMVTTEWAMYELAKNPK QDRLY +I++VCGS+KITEE L Q+PY++AIFHETLRRHSPV
Sbjct: 328  TMVTTEWAMYELAKNPKLQDRLYRDIKSVCGSEKITEEHLSQLPYITAIFHETLRRHSPV 387

Query: 383  PIIPLRYVHENTVLGGYHVPAGTELAINIYGCNMEREIWANPEEWNPERFLAENEPIDLQ 204
            PIIPLR+VHE+TVLGGYHVPAGTELA+NIYGCNM++ +W NPEEWNPERF+ ENE ID Q
Sbjct: 388  PIIPLRHVHEDTVLGGYHVPAGTELAVNIYGCNMDKNVWENPEEWNPERFMKENETIDFQ 447

Query: 203  RTMAFGGGKRVCAGAMQAMLIACVCMGRMVQEFEWRLKDDTGEDVNTIGLTTQMLNPMLA 24
            +TMAFGGGKRVCAG++QA+L A + +GRMVQEFEW+LKD T E+VNTIGLTTQML P+ A
Sbjct: 448  KTMAFGGGKRVCAGSLQALLTASIGIGRMVQEFEWKLKDMTQEEVNTIGLTTQMLRPLRA 507

Query: 23   VIKPR 9
            +IKPR
Sbjct: 508  IIKPR 512


>dbj|BAG71198.1| ent-kaurene oxidase 2 [Lactuca sativa]
          Length = 512

 Score =  744 bits (1921), Expect = 0.0
 Identities = 359/484 (74%), Positives = 419/484 (86%)
 Frame = -2

Query: 1460 LSFLILKSFLSHQRRNPNHLPTVPAVPGVPLLGNLLQLKEKKPYKTFTKWAETYGPIYSI 1281
            L F  L+S+ S    + NHLP VP VPGVP+LGNLLQLKEKKPY TFTKWAE YGPIYSI
Sbjct: 29   LYFWFLRSYASPSHHS-NHLPPVPEVPGVPVLGNLLQLKEKKPYMTFTKWAEMYGPIYSI 87

Query: 1280 KAGATSMVVVSSNHLAKEAMVTRFDSISTRKLSKALRILTADKTMVAMSDYDDYHKTVKR 1101
            + GATSMVVVSSN +AKE +VTRF SISTRKLS AL++LT DK+MVAMSDY DYHKTVKR
Sbjct: 88   RTGATSMVVVSSNEIAKEVVVTRFPSISTRKLSYALKVLTEDKSMVAMSDYHDYHKTVKR 147

Query: 1100 NILTSVLGPAARKKHRAHRDTMADNVSNQLHALAPTSHREAINLREILQSELFTLAFKQV 921
            +ILT+VLGP A+KK RAHRDTM +NVSN+LHA    +  + +NLR+I QS+LF LA KQ 
Sbjct: 148  HILTAVLGPNAQKKFRAHRDTMMENVSNELHAFFEKNPNQEVNLRKIFQSQLFGLAMKQA 207

Query: 920  MGKDFESIYVGDLGITMTKEEVFQVLVVDPMMGAIDVDWRDFFPYLRWIPNTSFEKKIEQ 741
            +GKD ESIYV DL  TM +EE+F+VLVVDPMMGAI+VDWRDFFPYL+W+PN SFE  I +
Sbjct: 208  LGKDVESIYVKDLETTMKREEIFEVLVVDPMMGAIEVDWRDFFPYLKWVPNKSFENIIHR 267

Query: 740  MDIRREAVMKTLIQEHRKRIDSGETLNSYIDYLLSEAQPLTEKQLLMSLWEPIIETSDTT 561
            M  RREAVMK LIQEH+KRI SGE LNSYIDYLLSEAQ LT+KQLLMSLWEPIIE+SDTT
Sbjct: 268  MYTRREAVMKALIQEHKKRIASGENLNSYIDYLLSEAQTLTDKQLLMSLWEPIIESSDTT 327

Query: 560  MVTTEWAMYELAKNPKKQDRLYDEIRNVCGSQKITEEKLCQMPYLSAIFHETLRRHSPVP 381
            MVTTEWAMYELAKNP  QDRLY+EI++VCGS+KITEE L Q+PYL A+F ETLR+H PVP
Sbjct: 328  MVTTEWAMYELAKNPNMQDRLYEEIQSVCGSEKITEENLSQLPYLYAVFQETLRKHCPVP 387

Query: 380  IIPLRYVHENTVLGGYHVPAGTELAINIYGCNMEREIWANPEEWNPERFLAENEPIDLQR 201
            I+PLRYVHENTVLGGYHVPAGTE+AINIYGCNM++++W NPEEWNPERFL+E E +DL +
Sbjct: 388  IMPLRYVHENTVLGGYHVPAGTEVAINIYGCNMDKKVWENPEEWNPERFLSEKESMDLYK 447

Query: 200  TMAFGGGKRVCAGAMQAMLIACVCMGRMVQEFEWRLKDDTGEDVNTIGLTTQMLNPMLAV 21
            TMAFGGGKRVCAG++QAM+I+C+ +GR+VQ+FEW+LKDD  EDVNT+GLTTQ L+P+LA+
Sbjct: 448  TMAFGGGKRVCAGSLQAMVISCIGIGRLVQDFEWKLKDDAEEDVNTLGLTTQKLHPLLAL 507

Query: 20   IKPR 9
            I PR
Sbjct: 508  INPR 511


>gb|AFD54196.1| ent-kaurene oxidase [Vitis vinifera]
          Length = 508

 Score =  645 bits (1663), Expect = 0.0
 Identities = 306/482 (63%), Positives = 391/482 (81%), Gaps = 1/482 (0%)
 Frame = -2

Query: 1451 LILKSFLSHQRRNPNHLPTVPAVPGVPLLGNLLQLKEKKPYKTFTKWAETYGPIYSIKAG 1272
            + ++ F+S+++R+   LP VP +PG+PL+GNLLQLKEKKP+KTF +WAETYGPI+SI+ G
Sbjct: 26   IFIRRFVSNRKRSVEGLPPVPDIPGLPLIGNLLQLKEKKPHKTFARWAETYGPIFSIRTG 85

Query: 1271 ATSMVVVSSNHLAKEAMVTRFDSISTRKLSKALRILTADKTMVAMSDYDDYHKTVKRNIL 1092
            A++M+V++S+ +AKEAMVTRF SISTRKLS AL+ILT DK MVA SDY+D+HK VK  IL
Sbjct: 86   ASTMIVLNSSEVAKEAMVTRFSSISTRKLSNALKILTFDKCMVATSDYNDFHKMVKGFIL 145

Query: 1091 TSVLGPAARKKHRAHRDTMADNVSNQLHALAPTSHREAINLREILQSELFTLAFKQVMGK 912
             +VLG  A+K+HR HRDT+ +N+S  LHA   TS  E + L++I +SE+F LA KQ +GK
Sbjct: 146  RNVLGAPAQKRHRCHRDTLIENISKYLHAHVKTSPLEPVVLKKIFESEIFGLALKQALGK 205

Query: 911  DFESIYVGDLGITMTKEEVFQVLVVDPMMGAIDVDWRDFFPYLRWIPNTSFEKKIEQMDI 732
            D ESIYV +LG T+++EE+F VLVVDPM GAI+VDWRDFFPYL WIPN S E KI++MD 
Sbjct: 206  DIESIYVEELGTTLSREEIFAVLVVDPMAGAIEVDWRDFFPYLSWIPNKSMEMKIQRMDF 265

Query: 731  RREAVMKTLIQEHRKRIDSGETLNSYIDYLLSEAQPLTEKQLLMSLWEPIIETSDTTMVT 552
            RR A+MK LI E +KRI SGE  NSYID+LLSEA  LTEKQ+ M +WE IIE SDTT+VT
Sbjct: 266  RRGALMKALIGEQKKRIGSGEEKNSYIDFLLSEATTLTEKQIAMLIWETIIEISDTTLVT 325

Query: 551  TEWAMYELAKNPKKQDRLYDEIRNVCGSQKITEEKLCQMPYLSAIFHETLRRHSPVPIIP 372
            +EWAMYELAK+P +Q+ LY EI  VCGS K+TEE L ++PYL+++FHETLR++SP P++P
Sbjct: 326  SEWAMYELAKDPNRQEILYREIHKVCGSNKLTEENLSKLPYLNSVFHETLRKYSPAPMVP 385

Query: 371  LRYVHENTVLGGYHVPAGTELAINIYGCNMEREIWANPEEWNPERFLAEN-EPIDLQRTM 195
            +RY HE+T LGGYH+PAG+++AINIYGCNM ++ W NPEEW PERFL E  + +DL +TM
Sbjct: 386  VRYAHEDTQLGGYHIPAGSQIAINIYGCNMNKKQWENPEEWKPERFLDEKYDLMDLHKTM 445

Query: 194  AFGGGKRVCAGAMQAMLIACVCMGRMVQEFEWRLKDDTGEDVNTIGLTTQMLNPMLAVIK 15
            AFGGGKRVCAGA+QAMLIAC  +GR VQEFEW+L     E+V+T+ LT+Q L+PM A+IK
Sbjct: 446  AFGGGKRVCAGALQAMLIACTSIGRFVQEFEWKLMGGEEENVDTVALTSQKLHPMQAIIK 505

Query: 14   PR 9
             R
Sbjct: 506  AR 507