BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes22_contig00006079
         (1332 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAA37130.1| gibberellin 3beta-hydroxylase [Lactuca sativa]        363   e-156
dbj|BAA37129.1| gibberelin 3beta-hydroxylase [Lactuca sativa]         358   e-156
dbj|BAG48321.1| gibberellin 3-oxidase2 [Chrysanthemum x morifolium]   365   e-154
gb|ACF71491.1| gibberellin 3-beta hydroxylase [Chrysanthemum x m...   351   e-151
dbj|BAG48320.1| gibberellin 3-oxidase1 [Chrysanthemum x morifolium]   358   e-148

>dbj|BAA37130.1| gibberellin 3beta-hydroxylase [Lactuca sativa]
          Length = 362

 Score =  363 bits (933), Expect(2) = e-156
 Identities = 180/213 (84%), Positives = 198/213 (92%), Gaps = 4/213 (1%)
 Frame = +1

Query: 622  ELIEEYKKEMNKLANRLMWVMLGSLGITRGDIKWAGPAGELKEACPALQLNSYPACPDPD 801
            ++IEEY KEMNKL+NR+MW++LGSLGI +GDIKWA   GE+KEAC ALQLNSYP+CPDPD
Sbjct: 151  DVIEEYNKEMNKLSNRVMWLILGSLGIKKGDIKWANQEGEIKEACAALQLNSYPSCPDPD 210

Query: 802  RAMGLAAHTDSTLLTILHQNNTSGLQVQREGFGWVTVPPIAGALVVNVGDLLHILSNGLY 981
            RAMGLAAHTDSTLLTILHQ+NTSGLQVQR G GWVTV PIAGALVVN+GDL+ ILSNGLY
Sbjct: 211  RAMGLAAHTDSTLLTILHQSNTSGLQVQR-GVGWVTVKPIAGALVVNIGDLIQILSNGLY 269

Query: 982  SSVLHRAIVNRTQHRLSVAYLYGPPSNVQISPLSKLTDNVHPPLYRPVTWSEYLGTKAKH 1161
            SSVLHRA+VNRTQHRLSVAYLYGPPSNV+I+PLSKLTDNVHPPLYRPVTWSEYLGTKAKH
Sbjct: 270  SSVLHRAMVNRTQHRLSVAYLYGPPSNVRIAPLSKLTDNVHPPLYRPVTWSEYLGTKAKH 329

Query: 1162 FNKALSFVRLCA--TMNGFVDA--NDHNSVLVG 1248
            FNKAL FVRLCA  TM+GFVDA  ++HNSVLVG
Sbjct: 330  FNKALLFVRLCAPITMSGFVDASTDNHNSVLVG 362



 Score =  217 bits (553), Expect(2) = e-156
 Identities = 107/155 (69%), Positives = 122/155 (78%)
 Frame = +3

Query: 87  MSLKPLDAFRLHPMNAERPLDLNSMNELPDSYAWSAVDGXXXXXXXXXXXXINLEDPNAI 266
           MSL+ LDAFR      E+ LDLNS+ ELP+SYAWS+ D             INL+D NA+
Sbjct: 1   MSLQQLDAFR------EKHLDLNSIKELPESYAWSSEDNCPSSSNSEPVPVINLKDSNAM 54

Query: 267 KHVGHACKTWGVFQVTNHGIPTALLDNMEAAGRKLFSLPIQQKLKAARPPDGISGYGVAR 446
           KHVG+ACKTWGVFQVTNH IP  LL+NMEA+GRKLFSLP QQKLKAAR  DG+SGYGVAR
Sbjct: 55  KHVGYACKTWGVFQVTNHDIPITLLNNMEASGRKLFSLPFQQKLKAARSLDGVSGYGVAR 114

Query: 447 ISSFFPKLMWSEGFTIIGSPYEHAEKLWPQGCRNF 551
           ISSFFPKLMWSEGFT+I SP++HA+KLWPQG  NF
Sbjct: 115 ISSFFPKLMWSEGFTLIDSPHQHAQKLWPQGSGNF 149


>dbj|BAA37129.1| gibberelin 3beta-hydroxylase [Lactuca sativa]
          Length = 363

 Score =  358 bits (920), Expect(2) = e-156
 Identities = 175/209 (83%), Positives = 186/209 (88%)
 Frame = +1

Query: 622  ELIEEYKKEMNKLANRLMWVMLGSLGITRGDIKWAGPAGELKEACPALQLNSYPACPDPD 801
            ++IE YK EMN LA RLMW+MLGSLG+T  D+KW G  G    +CPALQLNSYPACPDPD
Sbjct: 159  DVIEGYKHEMNNLAQRLMWLMLGSLGVTTEDVKWDGSQG----SCPALQLNSYPACPDPD 214

Query: 802  RAMGLAAHTDSTLLTILHQNNTSGLQVQREGFGWVTVPPIAGALVVNVGDLLHILSNGLY 981
            RAMGLAAHTDSTLLTIL+QNNTSGLQ  REG GWVTVPPI GALVVNVGDLLHILSNGLY
Sbjct: 215  RAMGLAAHTDSTLLTILYQNNTSGLQAHREGAGWVTVPPIPGALVVNVGDLLHILSNGLY 274

Query: 982  SSVLHRAIVNRTQHRLSVAYLYGPPSNVQISPLSKLTDNVHPPLYRPVTWSEYLGTKAKH 1161
             SVLHRA+VNRTQHRLSVAYLYGPPSNVQISPLSKLTD+VHPPLYRPVTWSEYLGTKAKH
Sbjct: 275  PSVLHRAMVNRTQHRLSVAYLYGPPSNVQISPLSKLTDHVHPPLYRPVTWSEYLGTKAKH 334

Query: 1162 FNKALSFVRLCATMNGFVDANDHNSVLVG 1248
            FNKALS VRLC  +NGFVDANDH+ V VG
Sbjct: 335  FNKALSSVRLCVPLNGFVDANDHSGVPVG 363



 Score =  220 bits (561), Expect(2) = e-156
 Identities = 108/157 (68%), Positives = 121/157 (77%), Gaps = 2/157 (1%)
 Frame = +3

Query: 87  MSLKPLDAFRLHPMNAERPLDLNSMNELPDSYAWSAVDGXXXXXXXXXXXX--INLEDPN 260
           M  +  DAF+ HPM  ++ LDL SM ELP+S+AW + DG              INL+DPN
Sbjct: 1   MHTRVADAFKAHPMVNQKHLDLKSMKELPESHAWLSQDGSPSYGSSSSEQVPVINLKDPN 60

Query: 261 AIKHVGHACKTWGVFQVTNHGIPTALLDNMEAAGRKLFSLPIQQKLKAARPPDGISGYGV 440
           A+K VGHACKTWGVFQVTNHG+PT LL+ MEAAGRKLF+LPIQQKLKAAR PDG+SGYGV
Sbjct: 61  AMKLVGHACKTWGVFQVTNHGVPTNLLEEMEAAGRKLFALPIQQKLKAARAPDGVSGYGV 120

Query: 441 ARISSFFPKLMWSEGFTIIGSPYEHAEKLWPQGCRNF 551
           ARISSFFPKLMWSEGFTIIGSPYEHA KLWP     F
Sbjct: 121 ARISSFFPKLMWSEGFTIIGSPYEHARKLWPNRYSRF 157


>dbj|BAG48321.1| gibberellin 3-oxidase2 [Chrysanthemum x morifolium]
          Length = 358

 Score =  365 bits (936), Expect(2) = e-154
 Identities = 174/209 (83%), Positives = 190/209 (90%)
 Frame = +1

Query: 622  ELIEEYKKEMNKLANRLMWVMLGSLGITRGDIKWAGPAGELKEACPALQLNSYPACPDPD 801
            E+++EY KEM KL NRLMW++LGSLGIT GD+KWAGPAG+LKEACPALQLNSYPACPDPD
Sbjct: 150  EVVDEYNKEMKKLTNRLMWLVLGSLGITEGDVKWAGPAGDLKEACPALQLNSYPACPDPD 209

Query: 802  RAMGLAAHTDSTLLTILHQNNTSGLQVQREGFGWVTVPPIAGALVVNVGDLLHILSNGLY 981
            RAMGLAAHTDS+LLTILHQNNTSGLQVQREG GWVTVPPI GALVV+VGDLLHILSNGLY
Sbjct: 210  RAMGLAAHTDSSLLTILHQNNTSGLQVQREGMGWVTVPPIKGALVVHVGDLLHILSNGLY 269

Query: 982  SSVLHRAIVNRTQHRLSVAYLYGPPSNVQISPLSKLTDNVHPPLYRPVTWSEYLGTKAKH 1161
            SSVLHRAIVNRTQHR S+AYLYGPPSNV+ISPLSKLT  V PPLY+PVTWSEYL  KAK+
Sbjct: 270  SSVLHRAIVNRTQHRFSIAYLYGPPSNVRISPLSKLTSIVRPPLYQPVTWSEYLAIKAKY 329

Query: 1162 FNKALSFVRLCATMNGFVDANDHNSVLVG 1248
            FNK LS VRL A ++GFVD  DH SV+VG
Sbjct: 330  FNKTLSVVRLSAPVDGFVDLKDHKSVIVG 358



 Score =  207 bits (526), Expect(2) = e-154
 Identities = 99/143 (69%), Positives = 113/143 (79%), Gaps = 1/143 (0%)
 Frame = +3

Query: 126 MNAERPLDLNSMNELPDSYAWSAVD-GXXXXXXXXXXXXINLEDPNAIKHVGHACKTWGV 302
           + AE  +DL+SM ELPDSYAW++VD G            INL+DPN +KHVGHACKTWGV
Sbjct: 6   LKAENHMDLSSMKELPDSYAWASVDDGCLLSCSSEPVPVINLDDPNVMKHVGHACKTWGV 65

Query: 303 FQVTNHGIPTALLDNMEAAGRKLFSLPIQQKLKAARPPDGISGYGVARISSFFPKLMWSE 482
           F VTNHGIP A++D+MEA+ RKLF LPIQQKLKAAR PDG+SGYG  RISSFF K MWSE
Sbjct: 66  FYVTNHGIPMAVVDDMEASVRKLFYLPIQQKLKAARAPDGVSGYGAVRISSFFHKHMWSE 125

Query: 483 GFTIIGSPYEHAEKLWPQGCRNF 551
           GFTIIGSPYEHA+KLWP   +NF
Sbjct: 126 GFTIIGSPYEHAKKLWPHDYKNF 148


>gb|ACF71491.1| gibberellin 3-beta hydroxylase [Chrysanthemum x morifolium]
          Length = 358

 Score =  351 bits (901), Expect(2) = e-151
 Identities = 168/208 (80%), Positives = 184/208 (88%)
 Frame = +1

Query: 625  LIEEYKKEMNKLANRLMWVMLGSLGITRGDIKWAGPAGELKEACPALQLNSYPACPDPDR 804
            +++EY KEM KL NRLMW++LGSLGIT GD+KWA    + KEACPALQLNSYPACPDPDR
Sbjct: 151  VVDEYNKEMKKLTNRLMWLVLGSLGITEGDVKWARQTRDTKEACPALQLNSYPACPDPDR 210

Query: 805  AMGLAAHTDSTLLTILHQNNTSGLQVQREGFGWVTVPPIAGALVVNVGDLLHILSNGLYS 984
            AMGLAAHTDS+LLTILHQNNTSGLQVQREG GWVTVPPI GALVV+VGDLLHILSNGLYS
Sbjct: 211  AMGLAAHTDSSLLTILHQNNTSGLQVQREGMGWVTVPPIKGALVVHVGDLLHILSNGLYS 270

Query: 985  SVLHRAIVNRTQHRLSVAYLYGPPSNVQISPLSKLTDNVHPPLYRPVTWSEYLGTKAKHF 1164
            SVLHRAIVNR QHRLS+AY+YGPPSNV+ISPLSKLT  V PPLY+PVTWSEYL  KAK+F
Sbjct: 271  SVLHRAIVNRAQHRLSIAYIYGPPSNVRISPLSKLTSIVRPPLYQPVTWSEYLAIKAKYF 330

Query: 1165 NKALSFVRLCATMNGFVDANDHNSVLVG 1248
            NK LS VRL A +NGFVD  DH SV+VG
Sbjct: 331  NKTLSIVRLSAPVNGFVDLKDHKSVIVG 358



 Score =  212 bits (540), Expect(2) = e-151
 Identities = 104/146 (71%), Positives = 114/146 (78%), Gaps = 1/146 (0%)
 Frame = +3

Query: 117 LHPMNAERPLDLNSMNELPDSYAWSAVDGXXXXXXXXXXXX-INLEDPNAIKHVGHACKT 293
           L P+ AE   DL+SM ELPDSYAWS+VD              INLEDPN +KHVGHACKT
Sbjct: 3   LKPLKAENHKDLSSMKELPDSYAWSSVDDDCLLSCSSEPVPVINLEDPNVMKHVGHACKT 62

Query: 294 WGVFQVTNHGIPTALLDNMEAAGRKLFSLPIQQKLKAARPPDGISGYGVARISSFFPKLM 473
           WGVF VTNHGIP A+LD+MEA+ RKLF LPIQQKLKAAR PDG+SGYG  RISSFF K M
Sbjct: 63  WGVFHVTNHGIPMAVLDDMEASVRKLFYLPIQQKLKAARAPDGVSGYGAVRISSFFHKHM 122

Query: 474 WSEGFTIIGSPYEHAEKLWPQGCRNF 551
           WSEGFTIIGSPYEHA+KLWPQ  +NF
Sbjct: 123 WSEGFTIIGSPYEHAKKLWPQDYKNF 148


>dbj|BAG48320.1| gibberellin 3-oxidase1 [Chrysanthemum x morifolium]
          Length = 356

 Score =  358 bits (920), Expect(2) = e-148
 Identities = 174/209 (83%), Positives = 187/209 (89%)
 Frame = +1

Query: 622  ELIEEYKKEMNKLANRLMWVMLGSLGITRGDIKWAGPAGELKEACPALQLNSYPACPDPD 801
            ++IEEYK+EMNKLA  LM +MLGSLG+T  D+KWAG  G    +CPALQLNSYPACPDPD
Sbjct: 152  DVIEEYKEEMNKLAQTLMSLMLGSLGVTMEDVKWAGSQG----SCPALQLNSYPACPDPD 207

Query: 802  RAMGLAAHTDSTLLTILHQNNTSGLQVQREGFGWVTVPPIAGALVVNVGDLLHILSNGLY 981
            RAMGLAAHTDSTLLTILHQNNTSGLQ  R+G GWVTVPPI GALV+NVGDLLHILSNGLY
Sbjct: 208  RAMGLAAHTDSTLLTILHQNNTSGLQAHRDGAGWVTVPPIPGALVINVGDLLHILSNGLY 267

Query: 982  SSVLHRAIVNRTQHRLSVAYLYGPPSNVQISPLSKLTDNVHPPLYRPVTWSEYLGTKAKH 1161
             SVLHRA+VNRT+HRLSVAYLYGPPSNVQISPLSKLTD VHPPLYRPVTWSEYLGTKAKH
Sbjct: 268  PSVLHRAMVNRTKHRLSVAYLYGPPSNVQISPLSKLTDQVHPPLYRPVTWSEYLGTKAKH 327

Query: 1162 FNKALSFVRLCATMNGFVDANDHNSVLVG 1248
            FNKALS VR+CA +NGF DANDHN V VG
Sbjct: 328  FNKALSSVRVCAPLNGFADANDHNGVQVG 356



 Score =  196 bits (497), Expect(2) = e-148
 Identities = 95/141 (67%), Positives = 109/141 (77%), Gaps = 2/141 (1%)
 Frame = +3

Query: 135 ERPLDLNSMNELPDSYAWSAVDGXXXXXXXXXXXX--INLEDPNAIKHVGHACKTWGVFQ 308
           ++ LDLNSM  LP+S+AW +++               I+L DPNA+  VGHACKTWGVFQ
Sbjct: 10  QKHLDLNSMKVLPESHAWLSLESSPLYSSLSPDLVPVISLTDPNAMNLVGHACKTWGVFQ 69

Query: 309 VTNHGIPTALLDNMEAAGRKLFSLPIQQKLKAARPPDGISGYGVARISSFFPKLMWSEGF 488
           VTNHG+P  LL+ MEAAGRKLF+LPIQQKLKAAR PDG+SGYGVARISSFFPKLMWSEGF
Sbjct: 70  VTNHGVPINLLEKMEAAGRKLFALPIQQKLKAARAPDGVSGYGVARISSFFPKLMWSEGF 129

Query: 489 TIIGSPYEHAEKLWPQGCRNF 551
           TI+GSPYEHA KLWP     F
Sbjct: 130 TIMGSPYEHALKLWPNSYTRF 150