BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes22_contig00005720
         (1253 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002277399.1| PREDICTED: E3 ubiquitin-protein ligase RHF2A...   308   5e-87
ref|XP_004139068.1| PREDICTED: E3 ubiquitin-protein ligase RHF2A...   301   1e-84
dbj|BAJ33931.1| unnamed protein product [Thellungiella halophila]     302   6e-83
ref|XP_002871983.1| hypothetical protein ARALYDRAFT_489047 [Arab...   300   6e-83
ref|NP_568410.1| E3 ubiquitin-protein ligase RHF2A [Arabidopsis ...   297   2e-82

>ref|XP_002277399.1| PREDICTED: E3 ubiquitin-protein ligase RHF2A [Vitis vinifera]
            gi|296084011|emb|CBI24399.3| unnamed protein product
            [Vitis vinifera]
          Length = 393

 Score =  308 bits (788), Expect(2) = 5e-87
 Identities = 178/337 (52%), Positives = 212/337 (62%), Gaps = 23/337 (6%)
 Frame = +1

Query: 265  LESFSESDPPTITGCKHEFHLQCILEWCQRSSQCPMCWQSISLKDPSSQELLDAIEQERS 444
            LE+F +SDP T+T CKHEFHLQCILEWCQRSSQCPMCWQ ISLKDP+SQELL+A+E+ER+
Sbjct: 39   LEAFCDSDPSTVTSCKHEFHLQCILEWCQRSSQCPMCWQPISLKDPTSQELLEAVERERT 98

Query: 445  IANNPSMNSTIFHHPTFGDFELQHLPVSATDAELEERIIQHLXXXXXXXXXXXXXXXEGH 624
               NPS N+TIFHHPT GDFELQHLPV A DAELEERIIQHL               EG 
Sbjct: 99   FRFNPSRNATIFHHPTLGDFELQHLPVGANDAELEERIIQHLAAAAAMGRARHIARREGQ 158

Query: 625  RSQTSVQGRPRYLGFS---------------AQXXXXXXXXXXXXDNVDAIGRTVHEESV 759
            R+++S QGRP++L FS               AQ                ++   V EES 
Sbjct: 159  RTRSSAQGRPQFLVFSAHPSTPQAGPASSSPAQRGEDEPAPAITAAMPSSLPTAVGEESS 218

Query: 760  RGSAPSSSVHASERPASPLISTIVPSRPHETPSTD-RSVMQSSPNSQDRAGPSELHSFSD 936
            R +    S  A E  AS   S I  +  H T S + RS  QS P+ QD+AGPSE  SFS+
Sbjct: 219  RLTTSQLSSQAEEITASASGSIIHTTNQHGTSSNNRRSPGQSLPSGQDKAGPSEFQSFSE 278

Query: 937  -FKTRVNAISTRYKESITKTTKSWKERLFSRNNSTADHGSTDTGEGSAGIATLSRMMDHL 1113
              K+R NA+S RYKESI+K+T+ WKERLFSRN S AD GS    E +AGIAT+SRMM+HL
Sbjct: 279  TLKSRFNAVSMRYKESISKSTRGWKERLFSRNTSMADIGSDVRREVNAGIATVSRMMEHL 338

Query: 1114 E----QDLAPVSNSIMATSTSSPS--DQRSQATTAND 1206
            E        P S S  +  TSSPS  DQR   T+A++
Sbjct: 339  ETRDNSRTTPSSVSDSSEDTSSPSLGDQRMAETSAHN 375



 Score = 41.2 bits (95), Expect(2) = 5e-87
 Identities = 20/26 (76%), Positives = 20/26 (76%)
 Frame = +3

Query: 144 MEEGTKSGNHLISAAAFVEGGTQEAC 221
           MEE  KS  HL SAAAFVEGG QEAC
Sbjct: 6   MEESNKSECHLTSAAAFVEGGIQEAC 31


>ref|XP_004139068.1| PREDICTED: E3 ubiquitin-protein ligase RHF2A-like [Cucumis sativus]
          Length = 389

 Score =  301 bits (770), Expect(2) = 1e-84
 Identities = 166/335 (49%), Positives = 208/335 (62%), Gaps = 18/335 (5%)
 Frame = +1

Query: 265  LESFSESDPPTITGCKHEFHLQCILEWCQRSSQCPMCWQSISLKDPSSQELLDAIEQERS 444
            LE+F +SDP T+T CKHEFHLQC+LEWCQRSSQCPMCWQSISLKDP+SQELL+A+EQERS
Sbjct: 39   LENFCDSDPSTMTSCKHEFHLQCVLEWCQRSSQCPMCWQSISLKDPTSQELLEAVEQERS 98

Query: 445  IANNPSMNSTIFHHPTFGDFELQHLPVSATDAELEERIIQHLXXXXXXXXXXXXXXXEGH 624
               NP+ NSTIF HPT GDFELQHLP  A DA+LEERI+QHL               E H
Sbjct: 99   FRLNPARNSTIFRHPTLGDFELQHLPAGANDADLEERILQHLAAAAAMGQARHSARRESH 158

Query: 625  RSQTSVQGRPRYLGFSAQXXXXXXXXXXXXDNVDAIGRTV-----------HEESVRGSA 771
            R+++ + GRP++L FS+             D        +            EE+ + +A
Sbjct: 159  RNRSGIHGRPQFLVFSSHPNSSSTSPSSGDDREGEAAPRIMVASISPPPPAGEEAPQSNA 218

Query: 772  PSSSVHASE-RPASPLISTIVPSRPHETPSTDRSVMQSSPNSQDRAGPSELHSFSD-FKT 945
              S V   +  P++P  S+   S+     S  RS  Q  PN+QDRAGPSEL SFSD  K+
Sbjct: 219  SVSPVQTDQSSPSAPQSSSASASQLGSPSSERRSSSQPVPNNQDRAGPSELQSFSDSLKS 278

Query: 946  RVNAISTRYKESITKTTKSWKERLFSRNNSTADHGSTDTGEGSAGIATLSRMMDHLE--- 1116
            R NA+S RYK+SITK+TK WKE+LFSRN S +D GS    E +AGIAT+SRMM+ LE   
Sbjct: 279  RFNAMSMRYKDSITKSTKGWKEKLFSRNTSMSDIGSEVRREVNAGIATVSRMMERLETRD 338

Query: 1117 --QDLAPVSNSIMATSTSSPSDQRSQATTANDSAS 1215
              +  +P S+S+   S  S S+ RS      +S S
Sbjct: 339  ARKSSSPESSSVGGNSPVSESNNRSMPENGGNSPS 373



 Score = 40.0 bits (92), Expect(2) = 1e-84
 Identities = 19/27 (70%), Positives = 21/27 (77%)
 Frame = +3

Query: 141 TMEEGTKSGNHLISAAAFVEGGTQEAC 221
           TM+E  KS  HL SAAAFVEGG Q+AC
Sbjct: 5   TMDETKKSEAHLTSAAAFVEGGIQDAC 31


>dbj|BAJ33931.1| unnamed protein product [Thellungiella halophila]
          Length = 378

 Score =  302 bits (774), Expect(2) = 6e-83
 Identities = 172/335 (51%), Positives = 212/335 (63%), Gaps = 15/335 (4%)
 Frame = +1

Query: 265  LESFSESDPPTITGCKHEFHLQCILEWCQRSSQCPMCWQSISLKDPSSQELLDAIEQERS 444
            LE+F ESDP T+T CKHE+HLQCILEWCQRSSQCPMCWQSISLKDP+SQELL+A+EQER+
Sbjct: 37   LEAFCESDPSTLTSCKHEYHLQCILEWCQRSSQCPMCWQSISLKDPTSQELLEAVEQERN 96

Query: 445  IANNPSMNSTIFHHPTFGDFELQHLPVSATDAELEERIIQHLXXXXXXXXXXXXXXXEGH 624
               NPS N+TIF HPT GDFELQHLPV   +AE+EERIIQHL               EGH
Sbjct: 97   FRFNPSRNATIFRHPTLGDFELQHLPVGVDNAEIEERIIQHLAAAAAMGRARHGARREGH 156

Query: 625  RSQTSVQGRPRYLGFSAQ-------XXXXXXXXXXXXDNVDAIGRTVHEESVRGSAPSSS 783
            RS++S QG P+++ FS+                    D+ D +    H  S   +    S
Sbjct: 157  RSRSSSQGHPQFMVFSSHPNASPPPPHPPMPSSPSQRDDSDTVTSLPH--SPNNTLGEGS 214

Query: 784  VHAS-ERPASPLISTIVPSRPHETPSTDRSVMQSSPNSQDRAGPSELHSFSD-FKTRVNA 957
            V ++ ++P S     + PS    + S  RS+ QSSP+ QDRAGPSEL SFSD  K+R+NA
Sbjct: 215  VQSNMQQPTSSHRRQVSPS---ASDSNSRSLNQSSPSDQDRAGPSELQSFSDSLKSRLNA 271

Query: 958  ISTRYKESITKTTKSWKERLFSRNNSTADHGSTDTGEGSAGIATLSRMMDHLEQ------ 1119
            +STRYKESI+K T++WK+R FSRN S AD GS    E SAGIAT+SRMMD LE       
Sbjct: 272  VSTRYKESISKNTRNWKDRFFSRNTSMADLGSEVKREVSAGIATVSRMMDRLEMKENSRP 331

Query: 1120 DLAPVSNSIMATSTSSPSDQRSQATTANDSASRNE 1224
            D A VSN   + + +  S+     + A D  S NE
Sbjct: 332  DTASVSNG--SENHTPESNNEHTRSEAGDGHSLNE 364



 Score = 33.1 bits (74), Expect(2) = 6e-83
 Identities = 15/22 (68%), Positives = 16/22 (72%)
 Frame = +3

Query: 156 TKSGNHLISAAAFVEGGTQEAC 221
           T    HL SAAAFVEGG Q+AC
Sbjct: 8   TTPEGHLTSAAAFVEGGIQDAC 29


>ref|XP_002871983.1| hypothetical protein ARALYDRAFT_489047 [Arabidopsis lyrata subsp.
            lyrata] gi|297317820|gb|EFH48242.1| hypothetical protein
            ARALYDRAFT_489047 [Arabidopsis lyrata subsp. lyrata]
          Length = 376

 Score =  300 bits (769), Expect(2) = 6e-83
 Identities = 168/333 (50%), Positives = 211/333 (63%), Gaps = 13/333 (3%)
 Frame = +1

Query: 265  LESFSESDPPTITGCKHEFHLQCILEWCQRSSQCPMCWQSISLKDPSSQELLDAIEQERS 444
            LESF ESDP T+T CKHE+HLQCILEWCQRSSQCPMCWQSISLKDP+SQELL+A+EQER+
Sbjct: 37   LESFCESDPSTLTSCKHEYHLQCILEWCQRSSQCPMCWQSISLKDPTSQELLEAVEQERN 96

Query: 445  IANNPSMNSTIFHHPTFGDFELQHLPVSATDAELEERIIQHLXXXXXXXXXXXXXXXEGH 624
               NP+ N+TIF HPT GDFELQHLPV   +AE+EERIIQHL               EGH
Sbjct: 97   FRFNPARNATIFRHPTLGDFELQHLPVGVDNAEIEERIIQHLAAAAAMGRARHGVRREGH 156

Query: 625  RSQTSVQGRPRYLGFSAQXXXXXXXXXXXXDN-------VDAIGRTVHEESVRGSAPSSS 783
            RS++S QG P+++ FS+              +        D +    H     GS  S+ 
Sbjct: 157  RSRSSSQGHPQFMVFSSHPNASSPPPHPPMPSSPSQRGESDTVSNLPHNTLGEGSHQSN- 215

Query: 784  VHASERPASPLISTIVPSRPHETPSTDRSVMQSSPNSQDRAGPSELHSFSD-FKTRVNAI 960
               ++ P S     + PS    + S  R V QSSP+ QDRAGPSEL SFS+  K+R+NA+
Sbjct: 216  ---TQPPTSSHPRQVSPS---ASDSNSRPVNQSSPSDQDRAGPSELQSFSESLKSRLNAV 269

Query: 961  STRYKESITKTTKSWKERLFSRNNSTADHGSTDTGEGSAGIATLSRMMDHLE--QDLAPV 1134
            STRYKESI+K T++WK+RLFSRN S AD GS    E SAGIAT+SRMM+ LE  ++  P 
Sbjct: 270  STRYKESISKNTRNWKDRLFSRNTSMADLGSEVKREVSAGIATVSRMMERLETRENSRPS 329

Query: 1135 SNSIMATS---TSSPSDQRSQATTANDSASRNE 1224
            + S+   S   T   +++ +  + A D  S NE
Sbjct: 330  TASVSDVSENHTPESNNEHNNRSEAGDEHSMNE 362



 Score = 35.0 bits (79), Expect(2) = 6e-83
 Identities = 16/22 (72%), Positives = 17/22 (77%)
 Frame = +3

Query: 156 TKSGNHLISAAAFVEGGTQEAC 221
           T S  HL SAAAFVEGG Q+AC
Sbjct: 8   TTSEGHLTSAAAFVEGGIQDAC 29


>ref|NP_568410.1| E3 ubiquitin-protein ligase RHF2A [Arabidopsis thaliana]
            gi|30688246|ref|NP_851050.1| E3 ubiquitin-protein ligase
            RHF2A [Arabidopsis thaliana]
            gi|75315838|sp|Q9ZT42.1|RHF2A_ARATH RecName: Full=E3
            ubiquitin-protein ligase RHF2A; AltName: Full=RING-H2
            zinc finger protein RHF2a
            gi|11762136|gb|AAG40346.1|AF324994_1 CIC7E11 [Arabidopsis
            thaliana] gi|3790587|gb|AAC69856.1| RING-H2 finger
            protein RHF2a [Arabidopsis thaliana]
            gi|13374859|emb|CAC34493.1| RING-H2 finger protein RHF2a
            [Arabidopsis thaliana] gi|26983856|gb|AAN86180.1| unknown
            protein [Arabidopsis thaliana]
            gi|332005583|gb|AED92966.1| E3 ubiquitin-protein ligase
            RHF2A [Arabidopsis thaliana] gi|332005584|gb|AED92967.1|
            E3 ubiquitin-protein ligase RHF2A [Arabidopsis thaliana]
          Length = 375

 Score =  297 bits (761), Expect(2) = 2e-82
 Identities = 167/332 (50%), Positives = 209/332 (62%), Gaps = 12/332 (3%)
 Frame = +1

Query: 265  LESFSESDPPTITGCKHEFHLQCILEWCQRSSQCPMCWQSISLKDPSSQELLDAIEQERS 444
            LESF ESDP T+T CKHE+HLQCILEWCQRSSQCPMCWQSISLKDP+SQELL+A+EQER+
Sbjct: 37   LESFCESDPSTLTSCKHEYHLQCILEWCQRSSQCPMCWQSISLKDPTSQELLEAVEQERN 96

Query: 445  IANNPSMNSTIFHHPTFGDFELQHLPVSATDAELEERIIQHLXXXXXXXXXXXXXXXEGH 624
               NP+ N+TIF HPT GDFELQHLPV   +AE+EERIIQHL               EGH
Sbjct: 97   FRFNPTRNATIFRHPTLGDFELQHLPVGVDNAEIEERIIQHLAAAAAMGRARHGVRREGH 156

Query: 625  RSQTSVQGRPRYLGFSAQ-------XXXXXXXXXXXXDNVDAIGRTVHEESVRGSAPSSS 783
            RS++S QG  +++ FS+Q                   D  D +    H     GS  S+ 
Sbjct: 157  RSRSSSQGHQQFMVFSSQPNASSPPPHPPMPSSPSQRDESDTVSNLPHNALGEGSHQSN- 215

Query: 784  VHASERPASPLISTIVPSRPHETPSTDRSVMQSSPNSQDRAGPSELHSFSD-FKTRVNAI 960
               ++ P S     + PS    + S  R + QSSP+ QDRAGPSEL SFS+  K+R+NA+
Sbjct: 216  ---TQPPTSSHPRQVSPS---ASDSNSRPLNQSSPSEQDRAGPSELQSFSESLKSRLNAV 269

Query: 961  STRYKESITKTTKSWKERLFSRNNSTADHGSTDTGEGSAGIATLSRMMDHLE--QDLAPV 1134
            STRYKESI+K T++WK+RLFSRN S AD GS    E SAGIAT+SRMM+ LE  ++  P 
Sbjct: 270  STRYKESISKNTRNWKDRLFSRNTSMADLGSEVKREVSAGIATVSRMMERLETRENSRPS 329

Query: 1135 SNSIMATSTSSPSDQRSQ--ATTANDSASRNE 1224
            + S+   S +   +  ++     A D  S NE
Sbjct: 330  TASVSDVSENHTPETNNEHNRAAAGDEHSVNE 361



 Score = 36.2 bits (82), Expect(2) = 2e-82
 Identities = 17/24 (70%), Positives = 18/24 (75%)
 Frame = +3

Query: 150 EGTKSGNHLISAAAFVEGGTQEAC 221
           E T S  HL SAAAFVEGG Q+AC
Sbjct: 6   ETTTSEGHLTSAAAFVEGGIQDAC 29