BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes22_contig00004871
         (1820 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAE16979.1| steroid 22-alpha-hydroxylase [Zinnia elegans]         813   0.0  
ref|XP_002267187.1| PREDICTED: cytochrome P450 90B1-like [Vitis ...   760   0.0  
emb|CBI17531.3| unnamed protein product [Vitis vinifera]              759   0.0  
ref|XP_002310224.1| cytochrome P450 probable campestanol to 6-de...   755   0.0  
ref|XP_003538851.1| PREDICTED: cytochrome P450 90B1-like [Glycin...   754   0.0  

>dbj|BAE16979.1| steroid 22-alpha-hydroxylase [Zinnia elegans]
          Length = 496

 Score =  813 bits (2101), Expect = 0.0
 Identities = 400/484 (82%), Positives = 428/484 (88%)
 Frame = +3

Query: 60   TNKMSDYEFFIFXXXXXXXXXXXXXXXKRRTQNTPTLNLPPGNMGWPFIGETIGYLRPYS 239
            T  M D EFFI                KRRT N  T NLPPGNMGWPFIGETIGYL+PYS
Sbjct: 5    TLNMCDLEFFILASCLVLALFLILKLVKRRTNNGSTRNLPPGNMGWPFIGETIGYLQPYS 64

Query: 240  ATTIGTFMDQHISRFGKIFKSNLFGEPTIVSVDPGLNRYILQNEGRLFECSYPRSIGGIL 419
            ATTIG FM+QHIS++GKI+KS+LFGEPTIVS DPGLN+YILQNEGRLFECSYPRSIGGIL
Sbjct: 65   ATTIGKFMEQHISKYGKIYKSSLFGEPTIVSADPGLNKYILQNEGRLFECSYPRSIGGIL 124

Query: 420  GKWSMLVLVGDMHRDMRLISLNFLTNARLKTHLLREVEKNTLWVLDSWQENSPFSAQDEA 599
            GKWSMLVLVGDMHRDMR ISLNFL+NARLKT L+ EVEKNTLWVLDSW+ENSPF AQ+EA
Sbjct: 125  GKWSMLVLVGDMHRDMRQISLNFLSNARLKTQLVNEVEKNTLWVLDSWKENSPFCAQEEA 184

Query: 600  KKFTFNLMAKHIMSLDPGKPETEQLKKEYVTFMKGVVSAPLNFPGTAYRKALKSRSTILK 779
            KKFTFNLMA HIMSLDPG+PETE+LKKEYVTFMKGVVS PLNFPGTAY KALKSR+TILK
Sbjct: 185  KKFTFNLMATHIMSLDPGEPETERLKKEYVTFMKGVVSPPLNFPGTAYWKALKSRATILK 244

Query: 780  FIEMRMEERIRKFDEGNDVEKLEDDLLGWVLKNSNLSKEQILDLVLSLLFAGHETSSVSI 959
            FIE +MEERIR  DEGN + KL++DLLGW +KNSNL+KEQILDLVLSLLFAGHETSSVSI
Sbjct: 245  FIETKMEERIR-MDEGNGLGKLDNDLLGWSMKNSNLTKEQILDLVLSLLFAGHETSSVSI 303

Query: 960  ALAIYFLEACPTAVHQLREEHEEIANAKKQSGVKDLSWDDYKKMEFTQCVINETLRFGNV 1139
            +LA+YFLEACPTAV QLREEHEEI   KK  G K L+WDDYKKMEFTQCVINETLRFGNV
Sbjct: 304  SLAVYFLEACPTAVRQLREEHEEIVMKKKLLGEKYLTWDDYKKMEFTQCVINETLRFGNV 363

Query: 1140 VRFLHRKAIKDVRYKGYDIPCGWKVLPVIAAVHLDPTIFDQPHLFNPWRWQNARVTSSSG 1319
            VRFLHRKAIKDVRYKGYDIPCGWKVLPVIAAVHLDPT FDQP+LF+PWRWQNA VTSS+ 
Sbjct: 364  VRFLHRKAIKDVRYKGYDIPCGWKVLPVIAAVHLDPTHFDQPYLFDPWRWQNASVTSSTC 423

Query: 1320 ATTTSPNNFMPFGGGPRLCTGSELAKLEMAIFIHHLVLKYHWELADSDAAFAYPYLDFPK 1499
            +T  S +NFMPFGGGPRLCTGSELAKLEMAIFIHHLVLKY WEL DSD AFAYPYLDFPK
Sbjct: 424  STPPSASNFMPFGGGPRLCTGSELAKLEMAIFIHHLVLKYEWELVDSDEAFAYPYLDFPK 483

Query: 1500 GLPI 1511
            GLPI
Sbjct: 484  GLPI 487


>ref|XP_002267187.1| PREDICTED: cytochrome P450 90B1-like [Vitis vinifera]
          Length = 487

 Score =  760 bits (1963), Expect = 0.0
 Identities = 377/483 (78%), Positives = 414/483 (85%), Gaps = 2/483 (0%)
 Frame = +3

Query: 69   MSDYEFFIFXXXXXXXXXXXXXXXKRRTQNTPTLNLPPGNMGWPFIGETIGYLRPYSATT 248
            MSD EFF+                KR+      LNLPPGN GWPF+GETIGYL+PYSAT+
Sbjct: 1    MSDLEFFLLLLASISVPLVILNLIKRKQSG---LNLPPGNTGWPFLGETIGYLKPYSATS 57

Query: 249  IGTFMDQHISRFGKIFKSNLFGEPTIVSVDPGLNRYILQNEGRLFECSYPRSIGGILGKW 428
            IG FM+QHISRFG+I+KSNLFGEPTIVS D GLNR+ILQNEG+LFECSYPRSIGGILGKW
Sbjct: 58   IGDFMEQHISRFGEIYKSNLFGEPTIVSADSGLNRFILQNEGKLFECSYPRSIGGILGKW 117

Query: 429  SMLVLVGDMHRDMRLISLNFLTNARLKTHLLREVEKNTLWVLDSWQENSPFSAQDEAKKF 608
            SMLVLVGDMHRDMR ISLNFL++ RL+THLL EV K+TL VL SW+EN  FSAQDEAKKF
Sbjct: 118  SMLVLVGDMHRDMRTISLNFLSHGRLRTHLLPEVVKHTLLVLSSWKENCTFSAQDEAKKF 177

Query: 609  TFNLMAKHIMSLDPGKPETEQLKKEYVTFMKGVVSAPLNFPGTAYRKALKSRSTILKFIE 788
            TFNLMAKHIMSLDPGKPETEQLKKEYVTFMKGVVSAPLNFPGTAYRKAL+SRSTILKFIE
Sbjct: 178  TFNLMAKHIMSLDPGKPETEQLKKEYVTFMKGVVSAPLNFPGTAYRKALQSRSTILKFIE 237

Query: 789  MRMEERIRKFDEGNDVEKLEDDLLGWVLKNSNLSKEQILDLVLSLLFAGHETSSVSIALA 968
            ++MEERI+K   G      +DDLLGWVLK+SNLS EQILDLVLSLLFAGHETSSV+IALA
Sbjct: 238  LKMEERIQKLRGGGFENMEDDDLLGWVLKHSNLSTEQILDLVLSLLFAGHETSSVAIALA 297

Query: 969  IYFLEACPTAVHQLREEHEEIANAKKQSGVKDLSWDDYKKMEFTQCVINETLRFGNVVRF 1148
            IYFLE CP AV QLREEH EI+ AKKQSG  +LSW+DYKKMEFTQCVI+ETLR GNVVRF
Sbjct: 298  IYFLEGCPNAVQQLREEHLEISRAKKQSGELELSWEDYKKMEFTQCVISETLRLGNVVRF 357

Query: 1149 LHRKAIKDVRYKGYDIPCGWKVLPVIAAVHLDPTIFDQPHLFNPWRWQN--ARVTSSSGA 1322
            LHRKA+KDVRYKGYDIPCGWKVLPVIAAVHLDP++FDQP  FNPWRWQN   R  S+S +
Sbjct: 358  LHRKALKDVRYKGYDIPCGWKVLPVIAAVHLDPSLFDQPQHFNPWRWQNNGNRGNSTSWS 417

Query: 1323 TTTSPNNFMPFGGGPRLCTGSELAKLEMAIFIHHLVLKYHWELADSDAAFAYPYLDFPKG 1502
            T T+  NFMPFGGGPRLC GSELAKLEMA+FIHHLVL Y+WEL D D AFA+P++DFPKG
Sbjct: 418  TATN-QNFMPFGGGPRLCAGSELAKLEMAVFIHHLVLNYNWELVDKDQAFAFPFVDFPKG 476

Query: 1503 LPI 1511
            LPI
Sbjct: 477  LPI 479


>emb|CBI17531.3| unnamed protein product [Vitis vinifera]
          Length = 488

 Score =  759 bits (1959), Expect = 0.0
 Identities = 374/483 (77%), Positives = 412/483 (85%), Gaps = 2/483 (0%)
 Frame = +3

Query: 69   MSDYEFFIFXXXXXXXXXXXXXXXKRRTQNTPTLNLPPGNMGWPFIGETIGYLRPYSATT 248
            MSD EFF+                KR+      LNLPPGN GWPF+GETIGYL+PYSAT+
Sbjct: 1    MSDLEFFLLLLASISVPLVILNLIKRKQSG---LNLPPGNTGWPFLGETIGYLKPYSATS 57

Query: 249  IGTFMDQHISRFGKIFKSNLFGEPTIVSVDPGLNRYILQNEGRLFECSYPRSIGGILGKW 428
            IG FM+QHISRFG+I+KSNLFGEPTIVS D GLNR+ILQNEG+LFECSYPRSIGGILGKW
Sbjct: 58   IGDFMEQHISRFGEIYKSNLFGEPTIVSADSGLNRFILQNEGKLFECSYPRSIGGILGKW 117

Query: 429  SMLVLVGDMHRDMRLISLNFLTNARLKTHLLREVEKNTLWVLDSWQENSPFSAQDEAKKF 608
            SMLVLVGDMHRDMR ISLNFL++ RL+THLL EV K+TL VL SW+EN  FSAQDEAKKF
Sbjct: 118  SMLVLVGDMHRDMRTISLNFLSHGRLRTHLLPEVVKHTLLVLSSWKENCTFSAQDEAKKF 177

Query: 609  TFNLMAKHIMSLDPGKPETEQLKKEYVTFMKGVVSAPLNFPGTAYRKALKSRSTILKFIE 788
            TFNLMAKHIMSLDPGKPETEQLKKEYVTFMKGVVSAPLNFPGTAYRKAL+SRSTILKFIE
Sbjct: 178  TFNLMAKHIMSLDPGKPETEQLKKEYVTFMKGVVSAPLNFPGTAYRKALQSRSTILKFIE 237

Query: 789  MRMEERIRKFDEGNDVEKLEDDLLGWVLKNSNLSKEQILDLVLSLLFAGHETSSVSIALA 968
            ++MEERI+K   G      +DDLLGWVLK+SNLS EQILDLVLSLLFAGHETSSV+IALA
Sbjct: 238  LKMEERIQKLRGGGFENMEDDDLLGWVLKHSNLSTEQILDLVLSLLFAGHETSSVAIALA 297

Query: 969  IYFLEACPTAVHQLREEHEEIANAKKQSGVKDLSWDDYKKMEFTQCVINETLRFGNVVRF 1148
            IYFLE CP AV QLREEH EI+ AKKQSG  +LSW+DYKKMEFTQCVI+ETLR GNVVRF
Sbjct: 298  IYFLEGCPNAVQQLREEHLEISRAKKQSGELELSWEDYKKMEFTQCVISETLRLGNVVRF 357

Query: 1149 LHRKAIKDVRYKGYDIPCGWKVLPVIAAVHLDPTIFDQPHLFNPWRWQ--NARVTSSSGA 1322
            LHRKA+KDVRYKGYDIPCGWKVLPVIAAVHLDP++FDQP  FNPWRWQ  N    +S+  
Sbjct: 358  LHRKALKDVRYKGYDIPCGWKVLPVIAAVHLDPSLFDQPQHFNPWRWQQNNGNRGNSTSW 417

Query: 1323 TTTSPNNFMPFGGGPRLCTGSELAKLEMAIFIHHLVLKYHWELADSDAAFAYPYLDFPKG 1502
            +T +  NFMPFGGGPRLC GSELAKLEMA+FIHHLVL Y+WEL D D AFA+P++DFPKG
Sbjct: 418  STATNQNFMPFGGGPRLCAGSELAKLEMAVFIHHLVLNYNWELVDKDQAFAFPFVDFPKG 477

Query: 1503 LPI 1511
            LPI
Sbjct: 478  LPI 480


>ref|XP_002310224.1| cytochrome P450 probable campestanol to 6-deoxocathasterone or
            6-oxocampestanol to cathasterone [Populus trichocarpa]
            gi|222853127|gb|EEE90674.1| cytochrome P450 probable
            campestanol to 6-deoxocathasterone or 6-oxocampestanol to
            cathasterone [Populus trichocarpa]
          Length = 486

 Score =  755 bits (1949), Expect = 0.0
 Identities = 368/451 (81%), Positives = 407/451 (90%), Gaps = 4/451 (0%)
 Frame = +3

Query: 171  NLPPGNMGWPFIGETIGYLRPYSATTIGTFMDQHISRFGKIFKSNLFGEPTIVSVDPGLN 350
            NLPPGNMGWPF+GETIGYL+PYSAT+IG FM+QHISR+GKI+KSNLFGEPTIVS D GLN
Sbjct: 32   NLPPGNMGWPFLGETIGYLKPYSATSIGEFMEQHISRYGKIYKSNLFGEPTIVSADAGLN 91

Query: 351  RYILQNEGRLFECSYPRSIGGILGKWSMLVLVGDMHRDMRLISLNFLTNARLKTHLLREV 530
            R+ILQNEGRLFECSYP+SIGGILGKWSMLVLVGDMHRDMR+ISLNFL++ARL+THLL+EV
Sbjct: 92   RFILQNEGRLFECSYPKSIGGILGKWSMLVLVGDMHRDMRIISLNFLSHARLRTHLLKEV 151

Query: 531  EKNTLWVLDSWQENSPFSAQDEAKKFTFNLMAKHIMSLDPGKPETEQLKKEYVTFMKGVV 710
            EK TL VL SW+EN  FSAQDEAKKFTFNLMAKHIMSLDPGKPETEQLKKEYVTFMKGVV
Sbjct: 152  EKQTLLVLSSWKENCTFSAQDEAKKFTFNLMAKHIMSLDPGKPETEQLKKEYVTFMKGVV 211

Query: 711  SAPLNFPGTAYRKALKSRSTILKFIEMRMEERIRKFDEGNDVEKLE-DDLLGWVLKNSNL 887
            SAP+N PGT YRKALKSRS ILKFIE +MEERI +   G  VE LE DDLLGWVLK+SNL
Sbjct: 212  SAPINLPGTPYRKALKSRSIILKFIERKMEERIAETKGG--VENLEDDDLLGWVLKHSNL 269

Query: 888  SKEQILDLVLSLLFAGHETSSVSIALAIYFLEACPTAVHQLREEHEEIANAKKQSGVKDL 1067
            S EQILDL+LSLLFAGHETSSVSIALAIYFL+ACP A+ QL+EEH +I+ AKK+SG  +L
Sbjct: 270  STEQILDLILSLLFAGHETSSVSIALAIYFLQACPGAIQQLKEEHIQISRAKKRSGETEL 329

Query: 1068 SWDDYKKMEFTQCVINETLRFGNVVRFLHRKAIKDVRYKGYDIPCGWKVLPVIAAVHLDP 1247
            +WDDYKKMEFTQCVI+ETLR GNVVRFLHRKA+KDVRYKGYDIPCGWKVLPVI+AVHLD 
Sbjct: 330  NWDDYKKMEFTQCVISETLRLGNVVRFLHRKALKDVRYKGYDIPCGWKVLPVISAVHLDS 389

Query: 1248 TIFDQPHLFNPWRWQ---NARVTSSSGATTTSPNNFMPFGGGPRLCTGSELAKLEMAIFI 1418
            T+FDQP  FNPWRWQ   NAR  S+S + TTS N FMPFGGGPRLC GSELAKLEMA+FI
Sbjct: 390  TLFDQPQHFNPWRWQQHNNARGASTSSSGTTSSNYFMPFGGGPRLCAGSELAKLEMAVFI 449

Query: 1419 HHLVLKYHWELADSDAAFAYPYLDFPKGLPI 1511
            HHLVL +HWEL D+D AFA+P++DFPKGLPI
Sbjct: 450  HHLVLNFHWELVDTDQAFAFPFVDFPKGLPI 480


>ref|XP_003538851.1| PREDICTED: cytochrome P450 90B1-like [Glycine max]
          Length = 489

 Score =  754 bits (1947), Expect = 0.0
 Identities = 358/456 (78%), Positives = 404/456 (88%)
 Frame = +3

Query: 144  RRTQNTPTLNLPPGNMGWPFIGETIGYLRPYSATTIGTFMDQHISRFGKIFKSNLFGEPT 323
            +R Q+ P LNLPPGNMGWPF+GETIGYL+PYSATTIG FM+QHI+R+G I+KS LFGEP 
Sbjct: 27   KRKQSKPRLNLPPGNMGWPFLGETIGYLKPYSATTIGEFMEQHIARYGTIYKSKLFGEPA 86

Query: 324  IVSVDPGLNRYILQNEGRLFECSYPRSIGGILGKWSMLVLVGDMHRDMRLISLNFLTNAR 503
            IVS D GLNR+ILQNEG+LFECSYPRSIGGILGKWSMLVLVGDMHRDMR+ISLNFL++AR
Sbjct: 87   IVSADAGLNRFILQNEGKLFECSYPRSIGGILGKWSMLVLVGDMHRDMRVISLNFLSHAR 146

Query: 504  LKTHLLREVEKNTLWVLDSWQENSPFSAQDEAKKFTFNLMAKHIMSLDPGKPETEQLKKE 683
            L+THLL+EVEK +L VL++W +NS FSAQDEAKKFTFNLMAKHIMS+DPG  ETE LKKE
Sbjct: 147  LRTHLLKEVEKQSLLVLNTWNQNSTFSAQDEAKKFTFNLMAKHIMSMDPGDIETEHLKKE 206

Query: 684  YVTFMKGVVSAPLNFPGTAYRKALKSRSTILKFIEMRMEERIRKFDEGNDVEKLEDDLLG 863
            YVTFMKGVVSAPLN PGTAYRKALKSRS ILKFIE +MEER+R+  EGN+  + EDDLL 
Sbjct: 207  YVTFMKGVVSAPLNLPGTAYRKALKSRSIILKFIEGKMEERVRRIQEGNESLE-EDDLLN 265

Query: 864  WVLKNSNLSKEQILDLVLSLLFAGHETSSVSIALAIYFLEACPTAVHQLREEHEEIANAK 1043
            WVLKNSNLS EQILDL+LSLLFAGHETSSV+IALAIYFL  CP A+ QL+EEH EIA AK
Sbjct: 266  WVLKNSNLSTEQILDLILSLLFAGHETSSVAIALAIYFLPGCPQAIQQLKEEHREIARAK 325

Query: 1044 KQSGVKDLSWDDYKKMEFTQCVINETLRFGNVVRFLHRKAIKDVRYKGYDIPCGWKVLPV 1223
            KQ+G  +L+WDDYK+MEFT CV+NETLR GNVVRFLHRKA+KDV YKGYDIPCGWKVLPV
Sbjct: 326  KQAGEVELTWDDYKRMEFTHCVVNETLRLGNVVRFLHRKAVKDVNYKGYDIPCGWKVLPV 385

Query: 1224 IAAVHLDPTIFDQPHLFNPWRWQNARVTSSSGATTTSPNNFMPFGGGPRLCTGSELAKLE 1403
            IAAVHLDP++FDQP  FNPWRWQN     S  +  T+ NNF+PFGGGPRLC GSELAKLE
Sbjct: 386  IAAVHLDPSLFDQPQHFNPWRWQNNGSHGSCPSKNTANNNFLPFGGGPRLCAGSELAKLE 445

Query: 1404 MAIFIHHLVLKYHWELADSDAAFAYPYLDFPKGLPI 1511
            MA+FIHHL+L YHWELAD+D AFAYP++DFPKGLP+
Sbjct: 446  MAVFIHHLILNYHWELADTDQAFAYPFVDFPKGLPV 481