BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes22_contig00004704
         (1500 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ADG41743.1| putative progesterone 5 beta-reductase [Nicotiana...   451   e-162
gb|AEX31545.1| putative steroid 5beta-reductase [Solanum lycoper...   459   e-161
gb|ADG46029.1| putative progesterone 5-beta-reductase [Corchorus...   449   e-161
dbj|BAH47640.1| progesterone 5beta reductase-A [Nicotiana tabacum]    447   e-161
dbj|BAH47641.1| progesterone 5beta reductase-B [Nicotiana tabacum]    448   e-161

>gb|ADG41743.1| putative progesterone 5 beta-reductase [Nicotiana tabacum]
          Length = 388

 Score =  451 bits (1159), Expect(2) = e-162
 Identities = 208/271 (76%), Positives = 230/271 (84%)
 Frame = +3

Query: 564  NGKMFKNVLDAVIPNSPNLQHVSLQTGRKHYLGPFESLGKVAHDPPFYEDLPRLDAPNFY 743
            NGKMF+NVL  +IPN PNL+H+ LQTGRKHYLGPFE  GKVAHD PF+EDLPRLDAPNFY
Sbjct: 119  NGKMFQNVLSVIIPNCPNLRHICLQTGRKHYLGPFELYGKVAHDSPFHEDLPRLDAPNFY 178

Query: 744  YTLEDILFKSVEQKEGLTWSVHRPGTIFGFSPYSMMNIIGALCVYATICKHEGVPFKFLG 923
            YTLED+LFK VE+KEGLTWSVHRPGTIFGFSPYS+MNI+G LCVYA ICKHEG+P KF G
Sbjct: 179  YTLEDVLFKEVEKKEGLTWSVHRPGTIFGFSPYSLMNIVGTLCVYAAICKHEGLPLKFPG 238

Query: 924  TKEAWDHYSDVSDADLIAEQHIWATVEPYAKNEAFNISNGDVFKWKHFWKVLAEQFEVEN 1103
             K AWD YSD SDADLIAE  IWA V+PYAKNEAFN+SNGDVFKWKHFWKVLAEQF VE 
Sbjct: 239  VKAAWDGYSDCSDADLIAEHQIWAAVDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFGVEA 298

Query: 1104 GGFDESGEKMSMVEMMKDKDEVWDEIVREKGLLGTKLEEVGVWWFVDTILGSECMLDTMN 1283
              FDE   + ++ EMMKDK  VWDEIV+E GL   KLE+VGVWWFVD IL  +C LDTMN
Sbjct: 299  AEFDEV-NRCTLAEMMKDKGPVWDEIVKENGLTLAKLEDVGVWWFVDLILAGDCPLDTMN 357

Query: 1284 KSKEHGFLGFRNSKSSLISWIDKMKGYKIVP 1376
            KSKEHGFLGFRNS+ + ISWIDK+K YK+VP
Sbjct: 358  KSKEHGFLGFRNSQKAFISWIDKVKAYKVVP 388



 Score =  147 bits (372), Expect(2) = e-162
 Identities = 66/76 (86%), Positives = 73/76 (96%)
 Frame = +1

Query: 235 DEDDAPPKYQSVALIVGVTGIVGNSLAEILPLSDTPGGPWKVYGLARRPRPPWNADHPID 414
           +EDDAPPKYQSVALI+GVTGIVGNSLAEILPL+DTPGGPWKVYG+ARRPRP WNADHPID
Sbjct: 17  EEDDAPPKYQSVALIIGVTGIVGNSLAEILPLADTPGGPWKVYGVARRPRPSWNADHPID 76

Query: 415 YIQCDISDPDQTLAKL 462
           YIQCDIS+P+ T +KL
Sbjct: 77  YIQCDISNPEDTQSKL 92


>gb|AEX31545.1| putative steroid 5beta-reductase [Solanum lycopersicum]
          Length = 387

 Score =  459 bits (1180), Expect(2) = e-161
 Identities = 210/271 (77%), Positives = 235/271 (86%)
 Frame = +3

Query: 564  NGKMFKNVLDAVIPNSPNLQHVSLQTGRKHYLGPFESLGKVAHDPPFYEDLPRLDAPNFY 743
            NGKMF+NVL+ +IPN PNL+H+ LQTGRKHYLGPFE  GKV+HDPPF+EDLPRLDAPNFY
Sbjct: 118  NGKMFRNVLNVIIPNCPNLRHICLQTGRKHYLGPFELYGKVSHDPPFHEDLPRLDAPNFY 177

Query: 744  YTLEDILFKSVEQKEGLTWSVHRPGTIFGFSPYSMMNIIGALCVYATICKHEGVPFKFLG 923
            Y LEDILFK VE+KEGLTWSVHRPGTIFGFSPYS+MNI+G LCVYA ICKHEG+P KF G
Sbjct: 178  YVLEDILFKEVEKKEGLTWSVHRPGTIFGFSPYSLMNIVGTLCVYAAICKHEGLPLKFPG 237

Query: 924  TKEAWDHYSDVSDADLIAEQHIWATVEPYAKNEAFNISNGDVFKWKHFWKVLAEQFEVEN 1103
             K AWD YSD SDADLIAE  IWA V+PYAKNEAFN+SNGDVFKWKHFWKVL EQF +E 
Sbjct: 238  VKGAWDGYSDCSDADLIAEHQIWAAVDPYAKNEAFNVSNGDVFKWKHFWKVLGEQFGLEA 297

Query: 1104 GGFDESGEKMSMVEMMKDKDEVWDEIVREKGLLGTKLEEVGVWWFVDTILGSECMLDTMN 1283
              FDE G++ ++VEMMKDK  VWDEIV+E GL+ TKLEEVGVWWFVD IL  +C LDTMN
Sbjct: 298  AEFDE-GKRCTLVEMMKDKGAVWDEIVKENGLVPTKLEEVGVWWFVDLILSGDCALDTMN 356

Query: 1284 KSKEHGFLGFRNSKSSLISWIDKMKGYKIVP 1376
            KSKEHGFLGFRNS+ + ISWIDK+K YK+VP
Sbjct: 357  KSKEHGFLGFRNSQKAFISWIDKVKAYKVVP 387



 Score =  139 bits (349), Expect(2) = e-161
 Identities = 60/76 (78%), Positives = 72/76 (94%)
 Frame = +1

Query: 235 DEDDAPPKYQSVALIVGVTGIVGNSLAEILPLSDTPGGPWKVYGLARRPRPPWNADHPID 414
           ++D+APPKY+SVALI+GVTGIVGNSLAEILPL+DTPGGPWKVYG+ARR RP WNADHPI+
Sbjct: 16  EDDEAPPKYESVALIIGVTGIVGNSLAEILPLADTPGGPWKVYGVARRARPSWNADHPIE 75

Query: 415 YIQCDISDPDQTLAKL 462
           Y+QCDIS+P+ T +KL
Sbjct: 76  YVQCDISNPEDTQSKL 91


>gb|ADG46029.1| putative progesterone 5-beta-reductase [Corchorus capsularis]
            gi|300391801|gb|ADK11283.1| putative progesterone
            5-beta-reductase [Corchorus olitorius]
          Length = 387

 Score =  449 bits (1156), Expect(2) = e-161
 Identities = 207/271 (76%), Positives = 231/271 (85%)
 Frame = +3

Query: 564  NGKMFKNVLDAVIPNSPNLQHVSLQTGRKHYLGPFESLGKVAHDPPFYEDLPRLDAPNFY 743
            NG MF+N+L AVIP+SPNL+H+ LQTGRKHYLGPFE  GKV HDPPF+EDLPRLD  NFY
Sbjct: 119  NGNMFRNLLSAVIPSSPNLRHICLQTGRKHYLGPFELFGKVGHDPPFHEDLPRLDVHNFY 178

Query: 744  YTLEDILFKSVEQKEGLTWSVHRPGTIFGFSPYSMMNIIGALCVYATICKHEGVPFKFLG 923
            YTLEDILF+ V++KEGLTWSVHRPG IFGFSPYS+MNI+G LCVYA ICKHEG P KF G
Sbjct: 179  YTLEDILFEEVQKKEGLTWSVHRPGNIFGFSPYSLMNIVGTLCVYAAICKHEGAPLKFPG 238

Query: 924  TKEAWDHYSDVSDADLIAEQHIWATVEPYAKNEAFNISNGDVFKWKHFWKVLAEQFEVEN 1103
             +EAWD YSD SDADLIAE HIWA V+PYAKNEAFN+SNGDVFKWKHFWKVLAEQF  E 
Sbjct: 239  CREAWDGYSDCSDADLIAEHHIWAAVDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFGAEC 298

Query: 1104 GGFDESGEKMSMVEMMKDKDEVWDEIVREKGLLGTKLEEVGVWWFVDTILGSECMLDTMN 1283
            G ++  G  +S+ EMMKDK  VWDEIVREKGL+ TKLEEVGVWWF D +L   C+LD MN
Sbjct: 299  GEYE--GGPLSLKEMMKDKGPVWDEIVREKGLVPTKLEEVGVWWFADVVLQYPCLLDAMN 356

Query: 1284 KSKEHGFLGFRNSKSSLISWIDKMKGYKIVP 1376
            KSKEHGFLGFRNSK++ ISWIDK+K YKIVP
Sbjct: 357  KSKEHGFLGFRNSKNAFISWIDKVKAYKIVP 387



 Score =  147 bits (372), Expect(2) = e-161
 Identities = 68/76 (89%), Positives = 71/76 (93%)
 Frame = +1

Query: 235 DEDDAPPKYQSVALIVGVTGIVGNSLAEILPLSDTPGGPWKVYGLARRPRPPWNADHPID 414
           DEDDAPP+YQSVALIVGVTGIVGNSLAEILPL+DTPGGPWKVYGLARRPRP WNADHPI 
Sbjct: 17  DEDDAPPQYQSVALIVGVTGIVGNSLAEILPLADTPGGPWKVYGLARRPRPSWNADHPIH 76

Query: 415 YIQCDISDPDQTLAKL 462
           YIQCDISDP  T +KL
Sbjct: 77  YIQCDISDPQDTQSKL 92


>dbj|BAH47640.1| progesterone 5beta reductase-A [Nicotiana tabacum]
          Length = 389

 Score =  447 bits (1151), Expect(2) = e-161
 Identities = 208/272 (76%), Positives = 231/272 (84%), Gaps = 1/272 (0%)
 Frame = +3

Query: 564  NGKMFKNVLDAVIPNSPNLQHVSLQTGRKHYLGPFESLGK-VAHDPPFYEDLPRLDAPNF 740
            NGKMF+NV+  VIPN PNL+H+ LQTGRKHYLGPFE  GK VAHD PF+EDLPRLDAPNF
Sbjct: 119  NGKMFQNVVSVVIPNCPNLRHICLQTGRKHYLGPFELYGKLVAHDSPFHEDLPRLDAPNF 178

Query: 741  YYTLEDILFKSVEQKEGLTWSVHRPGTIFGFSPYSMMNIIGALCVYATICKHEGVPFKFL 920
            YYTLED+LFK VE+KEGLTWSVHRPG IFGFSPYS+MNI+G LCVYA ICKHEG+P KF 
Sbjct: 179  YYTLEDVLFKEVEKKEGLTWSVHRPGVIFGFSPYSLMNIVGTLCVYAAICKHEGLPLKFP 238

Query: 921  GTKEAWDHYSDVSDADLIAEQHIWATVEPYAKNEAFNISNGDVFKWKHFWKVLAEQFEVE 1100
            G K AWD YSD SDADLIAE  IWA V+PYAKNEAFN+SNGDVFKWKHFWKVLAEQF VE
Sbjct: 239  GVKAAWDGYSDCSDADLIAEHQIWAAVDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFGVE 298

Query: 1101 NGGFDESGEKMSMVEMMKDKDEVWDEIVREKGLLGTKLEEVGVWWFVDTILGSECMLDTM 1280
               FDE   + ++VEMMKDK  VWDEIV+E GL  TKLE+VGVWWFVD +L  +C LDTM
Sbjct: 299  AAEFDEE-NRCTLVEMMKDKGPVWDEIVKENGLTPTKLEDVGVWWFVDLMLAGDCRLDTM 357

Query: 1281 NKSKEHGFLGFRNSKSSLISWIDKMKGYKIVP 1376
            NKSKEHGFLGFRNS+ + ISWIDK+K YK+VP
Sbjct: 358  NKSKEHGFLGFRNSQKAFISWIDKVKAYKVVP 389



 Score =  149 bits (375), Expect(2) = e-161
 Identities = 67/76 (88%), Positives = 73/76 (96%)
 Frame = +1

Query: 235 DEDDAPPKYQSVALIVGVTGIVGNSLAEILPLSDTPGGPWKVYGLARRPRPPWNADHPID 414
           +EDDAPPKYQSVALI+GVTGIVGNSLAEILPL+DTPGGPWKVYGLARRPRP WNADHPID
Sbjct: 17  EEDDAPPKYQSVALIIGVTGIVGNSLAEILPLADTPGGPWKVYGLARRPRPSWNADHPID 76

Query: 415 YIQCDISDPDQTLAKL 462
           YIQCDIS+P+ T +KL
Sbjct: 77  YIQCDISNPEDTQSKL 92


>dbj|BAH47641.1| progesterone 5beta reductase-B [Nicotiana tabacum]
          Length = 388

 Score =  448 bits (1153), Expect(2) = e-161
 Identities = 207/271 (76%), Positives = 229/271 (84%)
 Frame = +3

Query: 564  NGKMFKNVLDAVIPNSPNLQHVSLQTGRKHYLGPFESLGKVAHDPPFYEDLPRLDAPNFY 743
            NGKMF+NVL  +IPN PNL+H+ LQTGRKHYLGPFE  GKVAHD PF+EDLPRLDAPNFY
Sbjct: 119  NGKMFQNVLSVIIPNCPNLRHICLQTGRKHYLGPFELYGKVAHDFPFHEDLPRLDAPNFY 178

Query: 744  YTLEDILFKSVEQKEGLTWSVHRPGTIFGFSPYSMMNIIGALCVYATICKHEGVPFKFLG 923
            YTLED+LFK VE+KEGLTWSVHRPGTIFGFSPYS+MNI+G LCVYA ICKHEG+P KF G
Sbjct: 179  YTLEDVLFKEVEKKEGLTWSVHRPGTIFGFSPYSLMNIVGTLCVYAAICKHEGLPLKFPG 238

Query: 924  TKEAWDHYSDVSDADLIAEQHIWATVEPYAKNEAFNISNGDVFKWKHFWKVLAEQFEVEN 1103
             K AWD YSD SDADLIAE  IWA V+PYAKNEAFN+ NGDVFKWKHFWKVLAEQF VE 
Sbjct: 239  VKAAWDGYSDCSDADLIAEHQIWAAVDPYAKNEAFNVRNGDVFKWKHFWKVLAEQFGVEA 298

Query: 1104 GGFDESGEKMSMVEMMKDKDEVWDEIVREKGLLGTKLEEVGVWWFVDTILGSECMLDTMN 1283
              FDE   + ++ EMMKDK  VWDEIV+E GL   KLE+VGVWWFVD IL  +C LDTMN
Sbjct: 299  AEFDEV-NRCTLAEMMKDKGPVWDEIVKENGLTPAKLEDVGVWWFVDLILAGDCPLDTMN 357

Query: 1284 KSKEHGFLGFRNSKSSLISWIDKMKGYKIVP 1376
            KSKEHGFLGFRNS+ + ISWIDK+K YK+VP
Sbjct: 358  KSKEHGFLGFRNSQKAFISWIDKVKAYKVVP 388



 Score =  147 bits (372), Expect(2) = e-161
 Identities = 66/76 (86%), Positives = 73/76 (96%)
 Frame = +1

Query: 235 DEDDAPPKYQSVALIVGVTGIVGNSLAEILPLSDTPGGPWKVYGLARRPRPPWNADHPID 414
           +EDDAPPKYQSVALI+GVTGIVGNSLAEILPL+DTPGGPWKVYG+ARRPRP WNADHPID
Sbjct: 17  EEDDAPPKYQSVALIIGVTGIVGNSLAEILPLADTPGGPWKVYGVARRPRPSWNADHPID 76

Query: 415 YIQCDISDPDQTLAKL 462
           YIQCDIS+P+ T +KL
Sbjct: 77  YIQCDISNPEDTQSKL 92