BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes22_contig00003121
         (1321 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003632089.1| PREDICTED: cytochrome P450 76C1-like [Vitis ...   360   6e-97
emb|CAN71441.1| hypothetical protein VITISV_012452 [Vitis vinifera]   359   8e-97
gb|ABC94480.1| putative flavonoid 3'-hydroxylase cytochrome P450...   357   4e-96
gb|ABD15097.1| putative heme-binding cytochrome P450 [Artemisia ...   325   2e-86
ref|XP_002333806.1| cytochrome P450 [Populus trichocarpa] gi|222...   318   3e-84

>ref|XP_003632089.1| PREDICTED: cytochrome P450 76C1-like [Vitis vinifera]
          Length = 537

 Score =  360 bits (923), Expect = 6e-97
 Identities = 180/331 (54%), Positives = 239/331 (72%), Gaps = 1/331 (0%)
 Frame = -3

Query: 995  SSSNGAPSLPPGPRSLPIVGYLPFLGRELHTQFTNMAHTYGPIFKFHVGSKLHVVINTPD 816
            +S  G    PPGPR LP+VGYLPFLG  LH  F  +AH YGPIFK  +G+KL VV+++P 
Sbjct: 51   TSRKGTAPWPPGPRGLPVVGYLPFLGSNLHHSFAELAHLYGPIFKLWLGNKLCVVLSSPS 110

Query: 815  LAKVVVRDQDDTFANRNPSVAAKTITYGGQNIGWSNNNSYWRNLRKIFVHEVLSNKNLEA 636
            LAK VVRDQD  FANR+P VAA   TYGG +I WS   SYWRNLRK+FV E+LSN +LEA
Sbjct: 111  LAKQVVRDQDIIFANRDPPVAAFAYTYGGLDIAWSPYGSYWRNLRKVFVREMLSNTSLEA 170

Query: 635  CHSIRRDEVRKTIKNVHSKIGTPIDLREISFSTEANVLTSMVWENSSDKGGKDGHLGAEL 456
            C+ ++R EVRK I N +SKIGTP+D+ ++SF T  NV  S +W N+ +    DG +GAE 
Sbjct: 171  CYPLQRSEVRKAITNAYSKIGTPMDIGQLSFQTVVNVTLSSLWGNTPE-AHNDGKIGAEF 229

Query: 455  QMVVSKVVELLGQPNVSDFFPSLAWFDLQGVDRDMKRQLKHLDRIFASIIDDRIKSNSKK 276
            +   S++ ELLG+PN+SDFFP+LA FD+QGV+R MKR     +++  SIID ++K ++ K
Sbjct: 230  REAASEITELLGKPNISDFFPTLAGFDIQGVERQMKRAFLSAEQVIDSIIDRKMKKSTAK 289

Query: 275  LDDAAGHEAEKKDFLQILLELKDQKDA-TWLDITKIKALLMDVMVAGTETTATSIEYAMA 99
             ++ A    EKKDFLQ LL+LK Q+D  T + +T+IKALLMD++V GT+TTAT +E+ MA
Sbjct: 290  -EEGASDNGEKKDFLQFLLDLKVQEDTETPITMTQIKALLMDILVGGTDTTATMVEWVMA 348

Query: 98   EIMQNSDVMKSVQKELAEIVGPNNIVEESHL 6
            E+++N  +M   Q+EL  +VG  +IVEESHL
Sbjct: 349  EMIRNPVIMTRAQEELTNVVGMGSIVEESHL 379


>emb|CAN71441.1| hypothetical protein VITISV_012452 [Vitis vinifera]
          Length = 537

 Score =  359 bits (922), Expect = 8e-97
 Identities = 180/331 (54%), Positives = 239/331 (72%), Gaps = 1/331 (0%)
 Frame = -3

Query: 995  SSSNGAPSLPPGPRSLPIVGYLPFLGRELHTQFTNMAHTYGPIFKFHVGSKLHVVINTPD 816
            +S  G    PPGPR LP+VGYLPFLG  LH  F  +AH YGPIFK  +G+KL VV+++P 
Sbjct: 51   TSRKGXAPWPPGPRGLPVVGYLPFLGSNLHHSFAELAHLYGPIFKLWLGNKLCVVLSSPS 110

Query: 815  LAKVVVRDQDDTFANRNPSVAAKTITYGGQNIGWSNNNSYWRNLRKIFVHEVLSNKNLEA 636
            LAK VVRDQD  FANR+P VAA   TYGG +I WS   SYWRNLRK+FV E+LSN +LEA
Sbjct: 111  LAKQVVRDQDIIFANRDPPVAAFAYTYGGLDIAWSPYGSYWRNLRKVFVREMLSNTSLEA 170

Query: 635  CHSIRRDEVRKTIKNVHSKIGTPIDLREISFSTEANVLTSMVWENSSDKGGKDGHLGAEL 456
            C+ ++R EVRK I N +SKIGTP+D+ ++SF T  NV  S +W N+ +    DG +GAE 
Sbjct: 171  CYPLQRSEVRKAITNAYSKIGTPMDIGQLSFQTVVNVTLSSLWGNTPE-AHNDGKIGAEF 229

Query: 455  QMVVSKVVELLGQPNVSDFFPSLAWFDLQGVDRDMKRQLKHLDRIFASIIDDRIKSNSKK 276
            +   S++ ELLG+PN+SDFFP+LA FD+QGV+R MKR     +++  SIID ++K ++ K
Sbjct: 230  REAASEITELLGKPNISDFFPTLAGFDIQGVERQMKRAFLSAEQVIDSIIDRKMKKSTAK 289

Query: 275  LDDAAGHEAEKKDFLQILLELKDQKDA-TWLDITKIKALLMDVMVAGTETTATSIEYAMA 99
             ++ A    EKKDFLQ LL+LK Q+D  T + +T+IKALLMD++V GT+TTAT +E+ MA
Sbjct: 290  -EEGASDNGEKKDFLQFLLDLKVQEDTETPITMTQIKALLMDILVGGTDTTATMVEWVMA 348

Query: 98   EIMQNSDVMKSVQKELAEIVGPNNIVEESHL 6
            E+++N  +M   Q+EL  +VG  +IVEESHL
Sbjct: 349  EMIRNPVIMTRAQEELTNVVGMGSIVEESHL 379


>gb|ABC94480.1| putative flavonoid 3'-hydroxylase cytochrome P450 [Artemisia annua]
          Length = 528

 Score =  357 bits (916), Expect = 4e-96
 Identities = 179/324 (55%), Positives = 234/324 (72%), Gaps = 1/324 (0%)
 Frame = -3

Query: 971 LPPGPRSLPIVGYLPFLGRELHTQFTNMAHTYGPIFKFHVGSKLHVVINTPDLAKVVVRD 792
           LPPGP  LP++GYLPFL   LH +FT M+H YGPIF  ++GSKL+VV+N+ DLAKVV RD
Sbjct: 63  LPPGPYGLPVIGYLPFLSSNLHERFTEMSHKYGPIFSLYLGSKLNVVVNSIDLAKVVARD 122

Query: 791 QDDTFANRNPSVAAKTITYGGQNIGWSNNNSYWRNLRKIFVHEVLSNKNLEACHSIRRDE 612
            D TFANRNP V A T+TYG  +I WSNNN++WRN RK+ V +VLSN NL+AC   R DE
Sbjct: 123 LDQTFANRNPPVTAITVTYGLLDIAWSNNNTHWRNTRKLLVSQVLSNANLDACQGFRTDE 182

Query: 611 VRKTIKNVHSKIGTPIDLREISFSTEANVLTSMVWENSSDKGGKDGHLGAELQMVVSKVV 432
           VRKT+ +V++KIG  +D+ EISF TE NV+T+M+W  +      +G    EL     K++
Sbjct: 183 VRKTVGHVYAKIGEIVDINEISFETELNVVTNMLWGRNESGSLLEGFREVEL-----KML 237

Query: 431 ELLGQPNVSDFFPSLAWFDLQGVDRDMKRQLKHLDRIFASIIDDRIKSNSKKLDDAAGHE 252
           ELLG PN+SDF P L+WFDLQG  R+M++Q + LDRI  ++I  R+        +   H+
Sbjct: 238 ELLGAPNISDFIPMLSWFDLQGRKREMQKQHERLDRILDNVIKARM--------EGVLHD 289

Query: 251 AEKKDFLQILLELKDQKDA-TWLDITKIKALLMDVMVAGTETTATSIEYAMAEIMQNSDV 75
             KKDFLQI+LELKDQKD  T L++ +IKALL D++ A T+TT+T +E+ MAEI+ N DV
Sbjct: 290 DGKKDFLQIMLELKDQKDGPTSLNMVQIKALLFDILTASTDTTSTMVEWVMAEILHNPDV 349

Query: 74  MKSVQKELAEIVGPNNIVEESHLQ 3
           M+ VQ+EL  ++G NNIVEESHLQ
Sbjct: 350 MRRVQEELTIVIGMNNIVEESHLQ 373


>gb|ABD15097.1| putative heme-binding cytochrome P450 [Artemisia annua]
          Length = 534

 Score =  325 bits (833), Expect = 2e-86
 Identities = 167/332 (50%), Positives = 228/332 (68%), Gaps = 10/332 (3%)
 Frame = -3

Query: 971  LPPGPRSLPIVGYLPFLGRELHTQFTNMAHTYGPIFKFHVGSKLHVVINTPDLAKVVVRD 792
            LPPGP  LPIVGYLPFLG  LH +FTNMA TYGPIF   +G+KLHV++N+ DLAKV+  D
Sbjct: 49   LPPGPYGLPIVGYLPFLGSNLHEKFTNMADTYGPIFSLRLGTKLHVIVNSMDLAKVIAHD 108

Query: 791  QDDTFANRNPSVAAKTITYGGQNIGWSNNNSYWRNLRKIFVHEVLSNKNLEACHSIRRDE 612
             D TFANR P + A TITYG  ++ WSNNN++WR++RK+   +VLSN ++ AC   R   
Sbjct: 109  LDHTFANRVPPLTALTITYGASDVIWSNNNTHWRDMRKLLDSQVLSNTSINACQIFRTRA 168

Query: 611  VRKTIKNVHSKIGTPIDLREISFSTEANVLTSMVWENSSDKGGKDG-HLGAELQMVVSKV 435
            VR  +  V++KIGT +D+ +I+F T+ +++TSM+W  S    G D    G   + V  K+
Sbjct: 169  VRNMMNGVYAKIGTKVDINKIAFDTDLDLVTSMLWGCSEAGEGDDSIFTGGGFREVEYKI 228

Query: 434  VELLGQPNVSDFFPSLAWFDLQGVDRDMKRQLKHLDRIFASIIDDRIKSNSKKLDDAAGH 255
            VELLG PN+SD+ P L+ FDL G  R+M+RQL+++DR F +II +RIK NS+ + +    
Sbjct: 229  VELLGAPNISDYLPLLSCFDLHGRQREMQRQLEYVDRNFNNIIKERIKVNSRNI-ERGDK 287

Query: 254  EAEKKDFLQILLELKDQKD---------ATWLDITKIKALLMDVMVAGTETTATSIEYAM 102
            + E KDFLQILL+++DQKD         AT  +I  IKALL+D++ A T+ ++T +E+ M
Sbjct: 288  KDETKDFLQILLDIEDQKDTETSLNTDTATSFNIIHIKALLLDMVTAATDASSTMVEWVM 347

Query: 101  AEIMQNSDVMKSVQKELAEIVGPNNIVEESHL 6
            AEIM N  V K VQ EL  ++G  N+VEESHL
Sbjct: 348  AEIMHNPSVKKKVQDELTMVIG-KNVVEESHL 378


>ref|XP_002333806.1| cytochrome P450 [Populus trichocarpa] gi|222838666|gb|EEE77031.1|
            cytochrome P450 [Populus trichocarpa]
          Length = 533

 Score =  318 bits (814), Expect = 3e-84
 Identities = 153/325 (47%), Positives = 233/325 (71%)
 Frame = -3

Query: 980  APSLPPGPRSLPIVGYLPFLGRELHTQFTNMAHTYGPIFKFHVGSKLHVVINTPDLAKVV 801
            A  LPPGPR LPIVGYLPFLG  LH  F  +A TYGPI+K  +G KL V+I++P L K V
Sbjct: 53   ADKLPPGPRGLPIVGYLPFLGPNLHQMFMELALTYGPIYKLSIGRKLCVIISSPALVKEV 112

Query: 800  VRDQDDTFANRNPSVAAKTITYGGQNIGWSNNNSYWRNLRKIFVHEVLSNKNLEACHSIR 621
            VRDQD TFANRNP++AAKT +YGG++I +      WR LRKIFV E+ SN NL+A +S+R
Sbjct: 113  VRDQDITFANRNPTIAAKTFSYGGKDIAFQPYGPEWRMLRKIFVREMQSNANLDAFYSLR 172

Query: 620  RDEVRKTIKNVHSKIGTPIDLREISFSTEANVLTSMVWENSSDKGGKDGHLGAELQMVVS 441
            R++V++++   + KIG P+++ E++FST  N+++ M W  + +    +  +G+E +   S
Sbjct: 173  RNKVKESVNETYRKIGKPVNIGELAFSTVINMISGMFWGGTLEV-DTEIDIGSEFRAAAS 231

Query: 440  KVVELLGQPNVSDFFPSLAWFDLQGVDRDMKRQLKHLDRIFASIIDDRIKSNSKKLDDAA 261
            +++E+LG+PNVSDFFP LA FD+QG++R MK+  + +++I+  ++D+ I+    +++  A
Sbjct: 232  ELIEILGKPNVSDFFPVLARFDIQGIERKMKKATQRIEKIYDFVMDEWIEKGGARVESEA 291

Query: 260  GHEAEKKDFLQILLELKDQKDATWLDITKIKALLMDVMVAGTETTATSIEYAMAEIMQNS 81
             ++ ++KDF+  LL  K+Q     +   +IKALLMD++V GT+TT+T++E+AMAE+M + 
Sbjct: 292  KND-QRKDFMHFLLGFKEQDSGRSISREQIKALLMDIVVGGTDTTSTTVEWAMAEMMLHP 350

Query: 80   DVMKSVQKELAEIVGPNNIVEESHL 6
            +VMK+ QKEL + VG + IVEE H+
Sbjct: 351  EVMKNAQKELTDAVGTDEIVEERHI 375