BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes22_contig00001336
         (1912 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAJ17664.1| 4-coumarate:CoA ligase [Gynura bicolor]               980   0.0  
gb|AFL93685.1| 4-coumarate: coenzyme A ligase [Cynara cardunculu...   955   0.0  
gb|AEM63673.1| 4-hydroxycinnamoyl CoA ligase [Platycodon grandif...   917   0.0  
sp|O24146.1|4CL2_TOBAC RecName: Full=4-coumarate--CoA ligase 2; ...   900   0.0  
dbj|BAA07828.1| 4-coumarate:coenzyme A ligase [Nicotiana tabacum]     896   0.0  

>dbj|BAJ17664.1| 4-coumarate:CoA ligase [Gynura bicolor]
          Length = 542

 Score =  980 bits (2533), Expect = 0.0
 Identities = 487/538 (90%), Positives = 517/538 (96%)
 Frame = -3

Query: 1787 PEKEIIFRSKLPDIYIPKHLTLHSYCFENISSFSDRPCLIDGATGQVYTYADVELTSRKV 1608
            PEKEIIFRSKLPDIYIPKHL LHSYCFENIS FSDRPCLIDGA+GQ YTYADVELTSRKV
Sbjct: 5    PEKEIIFRSKLPDIYIPKHLPLHSYCFENISKFSDRPCLIDGASGQEYTYADVELTSRKV 64

Query: 1607 ASALHKQGINKGDVIMILLPNSAEFVFSFLGASYLGAISTMANPFFTAAEIIKQAKASSA 1428
            ASALH+QGINKGDVIMILLPNS EFV++F+GASYLGAISTMANPFFTAAEIIKQAKAS+A
Sbjct: 65   ASALHQQGINKGDVIMILLPNSPEFVYAFIGASYLGAISTMANPFFTAAEIIKQAKASNA 124

Query: 1427 KIIITQSSHVPKVKDFAADNSIKVVCIDPAPEGCLHYSELTSGDETELPEVEISSDDVVA 1248
            KIIITQS+HV KVK F+++NSI +VCID APEGCLHYSEL SGDE++LPEVEIS DDVVA
Sbjct: 125  KIIITQSAHVSKVKRFSSENSITIVCIDSAPEGCLHYSELLSGDESKLPEVEISPDDVVA 184

Query: 1247 LPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLWIHCEDVVICVLPLFHIYSLNSIL 1068
            LPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLWIH EDV+ICVLPLFHIYSLNSIL
Sbjct: 185  LPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLWIHSEDVLICVLPLFHIYSLNSIL 244

Query: 1067 LCGLRAGAAILIMQKFDIVPFLQLIEKYKVTIGPFVPPIVLTIANNEEVVDKYDLSSMRT 888
            LCGLRAGAAILIMQKFDIVPFLQLIEKYKVTIGPFVPPIVLTIANNE++VDKYDLSS+RT
Sbjct: 245  LCGLRAGAAILIMQKFDIVPFLQLIEKYKVTIGPFVPPIVLTIANNEKIVDKYDLSSIRT 304

Query: 887  VMSGAAPLGKELEDTVRMKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGTVV 708
            VMSGAAPLGKELEDTVRMKFPNAKLGQGYGMTEAGPVLAMCLAFAKEP+DIKSGACGTVV
Sbjct: 305  VMSGAAPLGKELEDTVRMKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYDIKSGACGTVV 364

Query: 707  RNAEMKIVDPETGASLPRNQRGEICIRGDQIMKGYVDDPEATKRTIDSEGWLHTGDIGYI 528
            RNAEMKIVDP+TG SLPRNQRGEICIRGDQIMKGY++DPE+TK TID++GWLHTGDIG I
Sbjct: 365  RNAEMKIVDPDTGLSLPRNQRGEICIRGDQIMKGYLNDPESTKNTIDADGWLHTGDIGLI 424

Query: 527  DDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPDISDAAVVPMINEAAGEVPVAFVV 348
            DDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPDISDAAVV M+N+AAGEVPVAFVV
Sbjct: 425  DDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPDISDAAVVSMVNDAAGEVPVAFVV 484

Query: 347  KSNGSGVTEDDIKQFISKQVVFYKRLKCVFFIDAIPKSPSGKILRKDLRAKLAASVPN 174
            K+N S VTED+IKQF+SKQVVFYKR+  VFFID IPKSPSGKILRKDLRAKLAA VPN
Sbjct: 485  KTNDSSVTEDEIKQFVSKQVVFYKRINRVFFIDTIPKSPSGKILRKDLRAKLAAGVPN 542


>gb|AFL93685.1| 4-coumarate: coenzyme A ligase [Cynara cardunculus var. scolymus]
          Length = 539

 Score =  955 bits (2468), Expect = 0.0
 Identities = 479/540 (88%), Positives = 510/540 (94%)
 Frame = -3

Query: 1793 MEPEKEIIFRSKLPDIYIPKHLTLHSYCFENISSFSDRPCLIDGATGQVYTYADVELTSR 1614
            ME + E IFRS+LPDIYIPKHL+LHSYCFENIS+FSDRPCLIDGATG VYTYADVELTSR
Sbjct: 1    MEAKSETIFRSRLPDIYIPKHLSLHSYCFENISAFSDRPCLIDGATGDVYTYADVELTSR 60

Query: 1613 KVASALHKQGINKGDVIMILLPNSAEFVFSFLGASYLGAISTMANPFFTAAEIIKQAKAS 1434
            KV SALHK GINKGDVIMILLPNS EFV+SFLGAS++GA+STMANPFFT+AEIIKQAKAS
Sbjct: 61   KVGSALHKHGINKGDVIMILLPNSPEFVYSFLGASFIGAVSTMANPFFTSAEIIKQAKAS 120

Query: 1433 SAKIIITQSSHVPKVKDFAADNSIKVVCIDPAPEGCLHYSELTSGDETELPEVEISSDDV 1254
            +AKIIITQS H  KVKDFA++NSIK+VCID APE CLH+SEL SGDE ELPEVEISSDDV
Sbjct: 121  AAKIIITQSEHAAKVKDFASENSIKLVCIDSAPEECLHFSELISGDEEELPEVEISSDDV 180

Query: 1253 VALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLWIHCEDVVICVLPLFHIYSLNS 1074
            VALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLWIH EDV++CVLPLFHIYSLNS
Sbjct: 181  VALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLWIHSEDVLMCVLPLFHIYSLNS 240

Query: 1073 ILLCGLRAGAAILIMQKFDIVPFLQLIEKYKVTIGPFVPPIVLTIANNEEVVDKYDLSSM 894
            ILLCGLRAGAAILIMQKF IVPFL+LI+KYKVTIGPFVPPIVLTIA NEEVVDKYDL+S+
Sbjct: 241  ILLCGLRAGAAILIMQKFHIVPFLELIQKYKVTIGPFVPPIVLTIAKNEEVVDKYDLTSI 300

Query: 893  RTVMSGAAPLGKELEDTVRMKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGT 714
            RTVM GAAPLGKELE+TVRMKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPF+IKSGACGT
Sbjct: 301  RTVMFGAAPLGKELENTVRMKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACGT 360

Query: 713  VVRNAEMKIVDPETGASLPRNQRGEICIRGDQIMKGYVDDPEATKRTIDSEGWLHTGDIG 534
            VVRNAEMKIVDP+TG SLPRNQRGEICIRGDQIMKGY++DPEATK TI  +GWLHTGDIG
Sbjct: 361  VVRNAEMKIVDPDTGVSLPRNQRGEICIRGDQIMKGYLNDPEATKSTI-VDGWLHTGDIG 419

Query: 533  YIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPDISDAAVVPMINEAAGEVPVAF 354
            YIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPDISDAAVVPM +E AGEVPVAF
Sbjct: 420  YIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPDISDAAVVPMKHEDAGEVPVAF 479

Query: 353  VVKSNGSGVTEDDIKQFISKQVVFYKRLKCVFFIDAIPKSPSGKILRKDLRAKLAASVPN 174
            VVKSNG+ VTE D+KQFISKQVVFYK+L  VFFID +PKSPSGKILRKDLRAKLAA VPN
Sbjct: 480  VVKSNGTTVTEVDVKQFISKQVVFYKKLYRVFFIDKVPKSPSGKILRKDLRAKLAAGVPN 539


>gb|AEM63673.1| 4-hydroxycinnamoyl CoA ligase [Platycodon grandiflorus]
          Length = 556

 Score =  917 bits (2371), Expect = 0.0
 Identities = 456/537 (84%), Positives = 501/537 (93%)
 Frame = -3

Query: 1784 EKEIIFRSKLPDIYIPKHLTLHSYCFENISSFSDRPCLIDGATGQVYTYADVELTSRKVA 1605
            ++EIIFRSKLPDIYIP HL LHSYCFENIS FS+RPCLI+GATG+VYTYADVE+TSRKVA
Sbjct: 21   KQEIIFRSKLPDIYIPNHLPLHSYCFENISKFSERPCLIEGATGKVYTYADVEVTSRKVA 80

Query: 1604 SALHKQGINKGDVIMILLPNSAEFVFSFLGASYLGAISTMANPFFTAAEIIKQAKASSAK 1425
            S+L+K GI +GD IM+LLPNSAEFVF+FLGASYLGAISTMANPFFT AEIIKQ KAS+AK
Sbjct: 81   SSLNKLGIGQGDTIMLLLPNSAEFVFAFLGASYLGAISTMANPFFTHAEIIKQVKASNAK 140

Query: 1424 IIITQSSHVPKVKDFAADNSIKVVCIDPAPEGCLHYSELTSGDETELPEVEISSDDVVAL 1245
            IIIT + HV KVK+FA++N +KV+CID APEGCLHYSELT GDE +LPEV+IS DDVVAL
Sbjct: 141  IIITLACHVDKVKEFASENDVKVICIDKAPEGCLHYSELTLGDENDLPEVKISPDDVVAL 200

Query: 1244 PYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLWIHCEDVVICVLPLFHIYSLNSILL 1065
            PYSSGTTGLPKGVMLTHKGLVTSVAQQVDGEN NL++H EDV+ICVLPLFHIYSLNSILL
Sbjct: 201  PYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLICVLPLFHIYSLNSILL 260

Query: 1064 CGLRAGAAILIMQKFDIVPFLQLIEKYKVTIGPFVPPIVLTIANNEEVVDKYDLSSMRTV 885
            CGLR GAAILIMQKFDIVPFL+LIEKYKVTIGPFVPPIVL +A +   VDKYDL+S+RTV
Sbjct: 261  CGLRVGAAILIMQKFDIVPFLELIEKYKVTIGPFVPPIVLAMAKSSH-VDKYDLTSIRTV 319

Query: 884  MSGAAPLGKELEDTVRMKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGTVVR 705
            MSGAAPLGKELEDTVR K PNAKLGQGYGMTEAGPVLAMCLAFAKEP++IKSGACGTVVR
Sbjct: 320  MSGAAPLGKELEDTVRAKLPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGTVVR 379

Query: 704  NAEMKIVDPETGASLPRNQRGEICIRGDQIMKGYVDDPEATKRTIDSEGWLHTGDIGYID 525
            NAEMKIVDP+TG SLPRNQRGEICIRGDQIMKGY++DPEAT+RTID EGWLHTGDIGYID
Sbjct: 380  NAEMKIVDPDTGDSLPRNQRGEICIRGDQIMKGYLNDPEATERTIDKEGWLHTGDIGYID 439

Query: 524  DDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPDISDAAVVPMINEAAGEVPVAFVVK 345
            DDDELFIVDRLKELIKYKGFQVAPAELEALLL HP+ISDAAVV MI+EAAGEVPVAFVV+
Sbjct: 440  DDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVSMIDEAAGEVPVAFVVR 499

Query: 344  SNGSGVTEDDIKQFISKQVVFYKRLKCVFFIDAIPKSPSGKILRKDLRAKLAASVPN 174
            +NGS +TED+IKQFISKQVVFYKR+  VFF D IPK+PSGKILRK+LRA+LAA +PN
Sbjct: 500  ANGSIITEDEIKQFISKQVVFYKRINRVFFTDTIPKAPSGKILRKELRARLAAGIPN 556


>sp|O24146.1|4CL2_TOBAC RecName: Full=4-coumarate--CoA ligase 2; Short=4CL 2; AltName:
            Full=4-coumaroyl-CoA synthase 2 gi|1663724|gb|AAB18638.1|
            4-coumarate:coenzyme A ligase [Nicotiana tabacum]
          Length = 542

 Score =  900 bits (2327), Expect = 0.0
 Identities = 445/535 (83%), Positives = 489/535 (91%)
 Frame = -3

Query: 1778 EIIFRSKLPDIYIPKHLTLHSYCFENISSFSDRPCLIDGATGQVYTYADVELTSRKVASA 1599
            +IIFRSKLPDIYIP HL LHSYCFENIS FS RPCLI+GA  Q+YTYADVEL SRKVA+ 
Sbjct: 9    DIIFRSKLPDIYIPNHLPLHSYCFENISEFSSRPCLINGANKQIYTYADVELNSRKVAAG 68

Query: 1598 LHKQGINKGDVIMILLPNSAEFVFSFLGASYLGAISTMANPFFTAAEIIKQAKASSAKII 1419
            LHKQGI   D IMILLPNS EFVF+F+GASYLGAISTMANP FT AE++KQAKASSAKII
Sbjct: 69   LHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAKII 128

Query: 1418 ITQSSHVPKVKDFAADNSIKVVCIDPAPEGCLHYSELTSGDETELPEVEISSDDVVALPY 1239
            +TQ+ HV KVKD+A +N +K++CID APEGCLH+S LT  +E ++PEVEI  DDVVALPY
Sbjct: 129  VTQACHVNKVKDYAFENDVKIICIDSAPEGCLHFSVLTQANEHDIPEVEIQPDDVVALPY 188

Query: 1238 SSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLWIHCEDVVICVLPLFHIYSLNSILLCG 1059
            SSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNL+IH EDV++CVLPLFHIYSLNS+LLCG
Sbjct: 189  SSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLFHIYSLNSVLLCG 248

Query: 1058 LRAGAAILIMQKFDIVPFLQLIEKYKVTIGPFVPPIVLTIANNEEVVDKYDLSSMRTVMS 879
            LR GAAILIMQKFDIV FL+LI++YKVTIGPFVPPIVL IA +  +VD YDLSS+RTVMS
Sbjct: 249  LRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKS-PMVDDYDLSSVRTVMS 307

Query: 878  GAAPLGKELEDTVRMKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGTVVRNA 699
            GAAPLGKELEDTVR KFPNAKLGQGYGMTEAGPVLAMCLAFAKEPF+IKSGACGTVVRNA
Sbjct: 308  GAAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACGTVVRNA 367

Query: 698  EMKIVDPETGASLPRNQRGEICIRGDQIMKGYVDDPEATKRTIDSEGWLHTGDIGYIDDD 519
            EMKIVDP+TG SLPRNQ GEICIRGDQIMKGY++DPEAT RTID EGWL+TGDIGYIDDD
Sbjct: 368  EMKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWLYTGDIGYIDDD 427

Query: 518  DELFIVDRLKELIKYKGFQVAPAELEALLLTHPDISDAAVVPMINEAAGEVPVAFVVKSN 339
            DELFIVDRLKELIKYKGFQVAPAELEALLL HP+ISDAAVVPM +E AGEVPVAFVV+SN
Sbjct: 428  DELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVPVAFVVRSN 487

Query: 338  GSGVTEDDIKQFISKQVVFYKRLKCVFFIDAIPKSPSGKILRKDLRAKLAASVPN 174
            GS +TED++K FISKQV+FYKR+K VFF+DAIPKSPSGKILRKDLRAKLAA +PN
Sbjct: 488  GSTITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKDLRAKLAAGLPN 542


>dbj|BAA07828.1| 4-coumarate:coenzyme A ligase [Nicotiana tabacum]
          Length = 542

 Score =  896 bits (2315), Expect = 0.0
 Identities = 442/535 (82%), Positives = 487/535 (91%)
 Frame = -3

Query: 1778 EIIFRSKLPDIYIPKHLTLHSYCFENISSFSDRPCLIDGATGQVYTYADVELTSRKVASA 1599
            +IIFRSKLPDIYIP HL LHSYCFENIS FS RPCLI+GA  Q+YTYADVEL+SRKVA+ 
Sbjct: 9    DIIFRSKLPDIYIPNHLPLHSYCFENISEFSSRPCLINGANKQIYTYADVELSSRKVAAG 68

Query: 1598 LHKQGINKGDVIMILLPNSAEFVFSFLGASYLGAISTMANPFFTAAEIIKQAKASSAKII 1419
            LHKQGI + D IMILLPNS EFVF+F+GASYLGAISTMANP FTAAE++KQ KAS AKII
Sbjct: 69   LHKQGIQQKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTAAEVVKQVKASGAKII 128

Query: 1418 ITQSSHVPKVKDFAADNSIKVVCIDPAPEGCLHYSELTSGDETELPEVEISSDDVVALPY 1239
            +TQ+ HV KVKD+A +N++K++CID APEGCLH+S LT  DE ++PEVEI  DDVVALPY
Sbjct: 129  VTQACHVNKVKDYALENNVKIICIDSAPEGCLHFSVLTQADEHDIPEVEIQPDDVVALPY 188

Query: 1238 SSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLWIHCEDVVICVLPLFHIYSLNSILLCG 1059
            SSGTTGLPKGVMLTHKGLVTSVAQQVDGEN NL+IH EDV++CVLPLFHIYSLNS+LLCG
Sbjct: 189  SSGTTGLPKGVMLTHKGLVTSVAQQVDGENRNLYIHSEDVLLCVLPLFHIYSLNSVLLCG 248

Query: 1058 LRAGAAILIMQKFDIVPFLQLIEKYKVTIGPFVPPIVLTIANNEEVVDKYDLSSMRTVMS 879
            LR GAAILIMQKFDIVPFL+LI+ YKVTIGPFVPPIVL IA +  +VD YDLSS+RTVMS
Sbjct: 249  LRVGAAILIMQKFDIVPFLELIQNYKVTIGPFVPPIVLAIAKS-PMVDDYDLSSVRTVMS 307

Query: 878  GAAPLGKELEDTVRMKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGTVVRNA 699
            GAAPLGKELEDTVR KFPNAKLGQGYGMTEAGPVLAMCLAFAKEPF+IKSGACGTVVRNA
Sbjct: 308  GAAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACGTVVRNA 367

Query: 698  EMKIVDPETGASLPRNQRGEICIRGDQIMKGYVDDPEATKRTIDSEGWLHTGDIGYIDDD 519
            EMKIVDPETG SLPRNQ GEICIRGDQIMKGY++DPEAT RTID EGWL+TGDIGYIDDD
Sbjct: 368  EMKIVDPETGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWLYTGDIGYIDDD 427

Query: 518  DELFIVDRLKELIKYKGFQVAPAELEALLLTHPDISDAAVVPMINEAAGEVPVAFVVKSN 339
            DELFIVDRLKELIKYKGFQVAPAELEALLL HP  SDAAVVPM +E A EVPVAFVV+S+
Sbjct: 428  DELFIVDRLKELIKYKGFQVAPAELEALLLNHPTFSDAAVVPMKDEQAEEVPVAFVVRSS 487

Query: 338  GSGVTEDDIKQFISKQVVFYKRLKCVFFIDAIPKSPSGKILRKDLRAKLAASVPN 174
            GS +TED++K FISKQV+FYKR+K VFF+DA+PKSPSGKILRKDLRAKLAA +PN
Sbjct: 488  GSTITEDEVKDFISKQVIFYKRIKRVFFVDAVPKSPSGKILRKDLRAKLAAGLPN 542