BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes22_contig00001312
         (859 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ACL10147.1| cytochrome P450 monooxygenase [Stevia rebaudiana]      303   3e-80
gb|ABD60225.1| ent-kaurenoic acid 13-hydroxylase [Stevia rebaudi...   302   6e-80
gb|ABC94482.1| putative taxane 13-alpha-hydroxylase cytochrome P...   253   5e-65
ref|XP_002299099.1| cytochrome P450 [Populus trichocarpa] gi|222...   222   1e-55
ref|XP_003632173.1| PREDICTED: LOW QUALITY PROTEIN: cytochrome P...   221   2e-55

>gb|ACL10147.1| cytochrome P450 monooxygenase [Stevia rebaudiana]
          Length = 476

 Score =  303 bits (776), Expect = 3e-80
 Identities = 147/204 (72%), Positives = 172/204 (84%)
 Frame = -1

Query: 859 AFATHYAVTMDSLTRRHIQIHWQGKEEVNVYKTVRLYAFELACRLFMSLEEPNHIAKLGS 680
           AFATHYAVTMD +TRRHI +HW+GKEEVNV++TV+LYAFELACRLFM+L++PNHIAKLGS
Sbjct: 140 AFATHYAVTMDVVTRRHIDVHWRGKEEVNVFQTVKLYAFELACRLFMNLDDPNHIAKLGS 199

Query: 679 LFNIFLKGVIELPLDFPGTRFYRTKKAAAAIRIQLMPIIKERRMALQEGKAXXXXXXXXX 500
           LFNIFLKG+IELP+D PGTRFY +KKAAAAIRI+L  +IK R++ L+EGK          
Sbjct: 200 LFNIFLKGIIELPIDVPGTRFYSSKKAAAAIRIELKKLIKARKLELKEGKPSSSQDLLSH 259

Query: 499 XXXXSDENGKYLTETEIANTILLLLFAGHDTSTVSITLVMKSLGEHPDVYDKVLREHLDI 320
                DENG +LTE EI + ILLLLFAGHDTS +SITL+MK+LGEH DVYDKVL+E L+I
Sbjct: 260 LLTSPDENGMFLTEEEIVDNILLLLFAGHDTSALSITLLMKALGEHSDVYDKVLKEQLEI 319

Query: 319 CKTKEAGELLKWEDIQKMRYSWSV 248
            K KEAGELLKWEDIQKM+YSWSV
Sbjct: 320 SKGKEAGELLKWEDIQKMKYSWSV 343



 Score =  139 bits (350), Expect = 8e-31
 Identities = 64/81 (79%), Positives = 72/81 (88%)
 Frame = -3

Query: 245 LREHLDICKTKEAGELLKWEDIQKMRYSWSVVSEVMRLNPPVTGAFREALVDFEYAGYTI 66
           L+E L+I K KEAGELLKWEDIQKM+YSWSVV EVMRLNPPV GA+REALVD +YAGYTI
Sbjct: 313 LKEQLEISKGKEAGELLKWEDIQKMKYSWSVVCEVMRLNPPVIGAYREALVDIDYAGYTI 372

Query: 65  PKGWKLYWSAASTHKDPAHFE 3
           PKGWKL+WSA ST +D A+FE
Sbjct: 373 PKGWKLHWSAVSTQRDEANFE 393


>gb|ABD60225.1| ent-kaurenoic acid 13-hydroxylase [Stevia rebaudiana]
           gi|189418962|gb|ACD93722.1| ent-kaurenoic acid
           13-hydroxylase [Stevia rebaudiana]
          Length = 476

 Score =  302 bits (774), Expect = 6e-80
 Identities = 147/204 (72%), Positives = 172/204 (84%)
 Frame = -1

Query: 859 AFATHYAVTMDSLTRRHIQIHWQGKEEVNVYKTVRLYAFELACRLFMSLEEPNHIAKLGS 680
           AFATHYAVTMD +TRRHI +HW+GKEEVNV++TV+LYAFELACRLFM+L++PNHIAKLGS
Sbjct: 140 AFATHYAVTMDVVTRRHIDVHWRGKEEVNVFQTVKLYAFELACRLFMNLDDPNHIAKLGS 199

Query: 679 LFNIFLKGVIELPLDFPGTRFYRTKKAAAAIRIQLMPIIKERRMALQEGKAXXXXXXXXX 500
           LFNIFLKG+IELP+D PGTRFY +KKAAAAIRI+L  +IK R++ L+EGKA         
Sbjct: 200 LFNIFLKGIIELPIDVPGTRFYSSKKAAAAIRIELKKLIKARKLELKEGKASSSQDLLSH 259

Query: 499 XXXXSDENGKYLTETEIANTILLLLFAGHDTSTVSITLVMKSLGEHPDVYDKVLREHLDI 320
                DENG +LTE EI + ILLLLFAGHDTS +SITL+MK+LGEH DVYDKVL+E L+I
Sbjct: 260 LLTSPDENGMFLTEEEIVDNILLLLFAGHDTSALSITLLMKTLGEHSDVYDKVLKEQLEI 319

Query: 319 CKTKEAGELLKWEDIQKMRYSWSV 248
            KTKEA E LKWEDIQKM+YSWSV
Sbjct: 320 SKTKEAWESLKWEDIQKMKYSWSV 343



 Score =  134 bits (338), Expect = 2e-29
 Identities = 61/81 (75%), Positives = 70/81 (86%)
 Frame = -3

Query: 245 LREHLDICKTKEAGELLKWEDIQKMRYSWSVVSEVMRLNPPVTGAFREALVDFEYAGYTI 66
           L+E L+I KTKEA E LKWEDIQKM+YSWSV+ EVMRLNPPV G +REALVD +YAGYTI
Sbjct: 313 LKEQLEISKTKEAWESLKWEDIQKMKYSWSVICEVMRLNPPVIGTYREALVDIDYAGYTI 372

Query: 65  PKGWKLYWSAASTHKDPAHFE 3
           PKGWKL+WSA ST +D A+FE
Sbjct: 373 PKGWKLHWSAVSTQRDEANFE 393


>gb|ABC94482.1| putative taxane 13-alpha-hydroxylase cytochrome P450 [Artemisia
           annua]
          Length = 480

 Score =  253 bits (645), Expect = 5e-65
 Identities = 126/205 (61%), Positives = 155/205 (75%), Gaps = 1/205 (0%)
 Frame = -1

Query: 859 AFATHYAVTMDSLTRRHIQIHWQGKEEVNVYKTVRLYAFELACRLFMSLEEPNHIAKLGS 680
           A +  Y  TM+ +TR HIQ HWQGK E+ V++TVR Y FELACRLF+SL++P H+A+LG+
Sbjct: 143 ALSNRYTGTMEVVTRLHIQNHWQGKSELKVFETVRPYLFELACRLFLSLDDPKHVAELGT 202

Query: 679 LFNIFLKGVIELPLDFPGTRFYRTKKAAAAIRIQLMPIIKERRMAL-QEGKAXXXXXXXX 503
           LFN FLKG+ ELP++ PGTRFYR K+AA AI+ QL+ IIK+RR AL QE ++        
Sbjct: 203 LFNTFLKGLTELPINIPGTRFYRAKRAANAIKKQLIVIIKQRRQALKQEDQSSSFEDLLS 262

Query: 502 XXXXXSDENGKYLTETEIANTILLLLFAGHDTSTVSITLVMKSLGEHPDVYDKVLREHLD 323
                SDENG++L+E EIAN +LLLLFAGHDTS VSITL+MKSL EHP VYD VL+E L 
Sbjct: 263 HLLVSSDENGRFLSEAEIANNVLLLLFAGHDTSAVSITLLMKSLAEHPQVYDNVLKEQLG 322

Query: 322 ICKTKEAGELLKWEDIQKMRYSWSV 248
           I + K  GE+L WEDIQKMRYSW V
Sbjct: 323 ILEAKAPGEMLNWEDIQKMRYSWYV 347



 Score =  124 bits (311), Expect = 3e-26
 Identities = 57/80 (71%), Positives = 64/80 (80%)
 Frame = -3

Query: 245 LREHLDICKTKEAGELLKWEDIQKMRYSWSVVSEVMRLNPPVTGAFREALVDFEYAGYTI 66
           L+E L I + K  GE+L WEDIQKMRYSW VV EVMRL PPV G+FREALVDFEYAGYTI
Sbjct: 317 LKEQLGILEAKAPGEMLNWEDIQKMRYSWYVVCEVMRLIPPVVGSFREALVDFEYAGYTI 376

Query: 65  PKGWKLYWSAASTHKDPAHF 6
           PKGWK+ WSA  THK+  +F
Sbjct: 377 PKGWKIIWSAVMTHKEENNF 396


>ref|XP_002299099.1| cytochrome P450 [Populus trichocarpa] gi|222846357|gb|EEE83904.1|
           cytochrome P450 [Populus trichocarpa]
          Length = 334

 Score =  222 bits (565), Expect = 1e-55
 Identities = 113/199 (56%), Positives = 140/199 (70%)
 Frame = -1

Query: 844 YAVTMDSLTRRHIQIHWQGKEEVNVYKTVRLYAFELACRLFMSLEEPNHIAKLGSLFNIF 665
           Y   MD +T+ HI   W+GKEEV V+ TV LY FEL+CRLF+S+++P HI+KL   F++F
Sbjct: 81  YIERMDLVTQHHISTLWEGKEEVKVHPTVNLYTFELSCRLFISIDDPLHISKLAHHFDVF 140

Query: 664 LKGVIELPLDFPGTRFYRTKKAAAAIRIQLMPIIKERRMALQEGKAXXXXXXXXXXXXXS 485
           LKGVI  P++ PGTRFYR  KAA AI+ +L  I + RR AL +  A             S
Sbjct: 141 LKGVIHFPINIPGTRFYRASKAADAIKEELRLISRRRRAALDKKMASPTQDLLSHLLVTS 200

Query: 484 DENGKYLTETEIANTILLLLFAGHDTSTVSITLVMKSLGEHPDVYDKVLREHLDICKTKE 305
           D +GK+L+ETEI + ILLLLFA HDT+T  IT VMK L E P+VY  VLRE +DI K+KE
Sbjct: 201 DASGKFLSETEIVDNILLLLFASHDTTTSVITCVMKYLAELPEVYQTVLREQIDIAKSKE 260

Query: 304 AGELLKWEDIQKMRYSWSV 248
            GELLKWEDIQKMRYSW+V
Sbjct: 261 PGELLKWEDIQKMRYSWNV 279



 Score =  119 bits (298), Expect = 9e-25
 Identities = 54/66 (81%), Positives = 60/66 (90%)
 Frame = -3

Query: 245 LREHLDICKTKEAGELLKWEDIQKMRYSWSVVSEVMRLNPPVTGAFREALVDFEYAGYTI 66
           LRE +DI K+KE GELLKWEDIQKMRYSW+VVSE+MRL PPV GAFREA+VDF YAGYTI
Sbjct: 249 LREQIDIAKSKEPGELLKWEDIQKMRYSWNVVSEIMRLMPPVRGAFREAIVDFTYAGYTI 308

Query: 65  PKGWKL 48
           PKGWK+
Sbjct: 309 PKGWKV 314


>ref|XP_003632173.1| PREDICTED: LOW QUALITY PROTEIN: cytochrome P450 716B2-like [Vitis
           vinifera]
          Length = 473

 Score =  221 bits (562), Expect = 2e-55
 Identities = 107/199 (53%), Positives = 144/199 (72%)
 Frame = -1

Query: 844 YAVTMDSLTRRHIQIHWQGKEEVNVYKTVRLYAFELACRLFMSLEEPNHIAKLGSLFNIF 665
           Y  TMD +T+ HI+  W+G EE+ VY  ++LY FELACRLF S+++P HI+KL + FN+F
Sbjct: 144 YVETMDLVTQHHIRTQWEGNEEMQVYPIIKLYTFELACRLFASMDDPGHISKLAAHFNVF 203

Query: 664 LKGVIELPLDFPGTRFYRTKKAAAAIRIQLMPIIKERRMALQEGKAXXXXXXXXXXXXXS 485
           LKG+I+ P++FPGT+F    KAA AIR +L  +  ERR+AL+E  A             +
Sbjct: 204 LKGIIDFPINFPGTKFXYATKAANAIREELRVMPMERRVALEEKTASATQDLLSHLLVTA 263

Query: 484 DENGKYLTETEIANTILLLLFAGHDTSTVSITLVMKSLGEHPDVYDKVLREHLDICKTKE 305
           D +G++LTE EI + ILLLLFAGHDT+  +ITL++K LGE P+VY KVLRE +DI ++KE
Sbjct: 264 DASGRFLTEMEIIDNILLLLFAGHDTTASAITLLIKYLGELPEVYAKVLREKIDITESKE 323

Query: 304 AGELLKWEDIQKMRYSWSV 248
            GELL+W+D QKMRYSW V
Sbjct: 324 PGELLQWKDXQKMRYSWHV 342



 Score =  129 bits (324), Expect = 8e-28
 Identities = 57/80 (71%), Positives = 66/80 (82%)
 Frame = -3

Query: 245 LREHLDICKTKEAGELLKWEDIQKMRYSWSVVSEVMRLNPPVTGAFREALVDFEYAGYTI 66
           LRE +DI ++KE GELL+W+D QKMRYSW V SEVMRL+PP +G+FREALVDF YAGY I
Sbjct: 312 LREKIDITESKEPGELLQWKDXQKMRYSWHVASEVMRLSPPASGSFREALVDFSYAGYNI 371

Query: 65  PKGWKLYWSAASTHKDPAHF 6
           PKGWKLYW   ST +DPA F
Sbjct: 372 PKGWKLYWGTGSTQRDPAFF 391