BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Atractylodes22_contig00001035
         (1431 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ACN65116.1| 2-alkenal reductase [Artemisia annua]                  530   e-148
ref|XP_002279359.1| PREDICTED: NADP-dependent alkenal double bon...   526   e-147
gb|ADO51748.1| alcohol dehydrogenase [Camellia sinensis]              525   e-146
ref|XP_002331649.1| predicted protein [Populus trichocarpa] gi|2...   509   e-142
pdb|4HFJ|A Chain A, X-ray Crystal Structure Of A Double Bond Red...   509   e-142

>gb|ACN65116.1| 2-alkenal reductase [Artemisia annua]
          Length = 347

 Score =  530 bits (1364), Expect = e-148
 Identities = 257/349 (73%), Positives = 300/349 (85%)
 Frame = +3

Query: 144  IEEGSPEVRNKQVIFKDYVYGYPKESDMVVTSDATIRLELPKNDGGLILSKNLYLSCDPY 323
            +E+    + NK+VI KDYV G+PKESDM++ +  T+ L+LP    GL++ KNLYLSCDPY
Sbjct: 1    MEQQQEVITNKKVILKDYVVGFPKESDMILKTSETMTLKLPAGSNGLLV-KNLYLSCDPY 59

Query: 324  MRGRMTKTFEGSYTTSFIPGSPISGYGVAKVLDSTHPDFKKGDLISGSTNWEEYSLITNP 503
            MR RMTKT EGSY  SF PGSP++GYGVAKVL+S H +FKKGDLI G T WEEYS+I  P
Sbjct: 60   MRSRMTKT-EGSYVESFTPGSPLTGYGVAKVLESGHANFKKGDLIWGFTGWEEYSIINAP 118

Query: 504  QSLIKIQHTDVPLSYHTGILGMAGMTAYVGFYEICSPKKGDTVFVSAASGAVGQLVGQFA 683
            + L KI+HTDVPLSY+TGILGM GMTAYVGFYEIC+PKKG+ VFVSAASGAVGQLVGQFA
Sbjct: 119  EGLFKIEHTDVPLSYYTGILGMPGMTAYVGFYEICTPKKGEYVFVSAASGAVGQLVGQFA 178

Query: 684  KLFGCYVVGSAGSKEKVDLLKNKFGFDEAFNYKEEQDLDAALKRYFPDGIDIYFENVGGK 863
            KL GCYVVGSAG+KEKVDLLKNKFGFDEAFNYKEEQDLDAALKRYFP+GIDIYFENVGG+
Sbjct: 179  KLSGCYVVGSAGTKEKVDLLKNKFGFDEAFNYKEEQDLDAALKRYFPEGIDIYFENVGGR 238

Query: 864  MLDEVLHNMRLHGRIAVCGMISQYNIDRHEGIYNLAFLILKRIRMQGFIVLDYYHLYPKF 1043
            MLD VL NMRL GRI+VCGMISQYN+++ EG+ NL  L+ KR+ M+GFIV D+YH YPK+
Sbjct: 239  MLDAVLLNMRLDGRISVCGMISQYNLEQSEGVRNLFTLVTKRVTMKGFIVFDHYHKYPKY 298

Query: 1044 LDMILPLIQERKITYVEDIVEGLENAPAALVGLFTGKNVGKQVVAIAHE 1190
            L+MI+PLI+   I Y+EDIVEGLENAPAAL+GL++GKNVGKQVV +AHE
Sbjct: 299  LEMIIPLIKNGTINYIEDIVEGLENAPAALIGLYSGKNVGKQVVVVAHE 347


>ref|XP_002279359.1| PREDICTED: NADP-dependent alkenal double bond reductase P2 [Vitis
            vinifera] gi|147792339|emb|CAN61471.1| hypothetical
            protein VITISV_043825 [Vitis vinifera]
          Length = 345

 Score =  526 bits (1355), Expect = e-147
 Identities = 258/342 (75%), Positives = 294/342 (85%)
 Frame = +3

Query: 165  VRNKQVIFKDYVYGYPKESDMVVTSDATIRLELPKNDGGLILSKNLYLSCDPYMRGRMTK 344
            V NKQVIF+DYV G+PKESDM VT+ +TI L++P+     +L KNLYLSCDPYMR RM +
Sbjct: 7    VSNKQVIFRDYVSGFPKESDMYVTT-STISLKVPEGSEA-VLVKNLYLSCDPYMRSRM-R 63

Query: 345  TFEGSYTTSFIPGSPISGYGVAKVLDSTHPDFKKGDLISGSTNWEEYSLITNPQSLIKIQ 524
              +GSY   F PGSPI+GYGVAKVLDS HPDF+ GDL+ G T WEEYSLIT  + L KIQ
Sbjct: 64   NIQGSYVEPFKPGSPITGYGVAKVLDSRHPDFRTGDLVWGITGWEEYSLITTDERLFKIQ 123

Query: 525  HTDVPLSYHTGILGMAGMTAYVGFYEICSPKKGDTVFVSAASGAVGQLVGQFAKLFGCYV 704
            HTDVPLSY+TGILGMAGMTAY GFYEICSPKKG+ VF+SAASGAVGQLVGQFAKL GCYV
Sbjct: 124  HTDVPLSYYTGILGMAGMTAYSGFYEICSPKKGEYVFISAASGAVGQLVGQFAKLLGCYV 183

Query: 705  VGSAGSKEKVDLLKNKFGFDEAFNYKEEQDLDAALKRYFPDGIDIYFENVGGKMLDEVLH 884
            VGSAG+KEKVDLLKNKFGFDEAFNYKEEQDL+A LKRYFP+GIDIYFENVGGKMLD VL 
Sbjct: 184  VGSAGTKEKVDLLKNKFGFDEAFNYKEEQDLEACLKRYFPEGIDIYFENVGGKMLDAVLV 243

Query: 885  NMRLHGRIAVCGMISQYNIDRHEGIYNLAFLILKRIRMQGFIVLDYYHLYPKFLDMILPL 1064
            NMRLHGRIAVCGMISQYN++  EG+ NL  ++ KRIRM+GF+V DYYHLYPKFLD+I+P 
Sbjct: 244  NMRLHGRIAVCGMISQYNLEEPEGVRNLFTIVTKRIRMEGFLVFDYYHLYPKFLDLIMPY 303

Query: 1065 IQERKITYVEDIVEGLENAPAALVGLFTGKNVGKQVVAIAHE 1190
            I+E KI YVEDI EGLE+AP AL+GL++G+NVGKQVV +A E
Sbjct: 304  IREGKIVYVEDIAEGLESAPTALIGLYSGRNVGKQVVVVARE 345


>gb|ADO51748.1| alcohol dehydrogenase [Camellia sinensis]
          Length = 347

 Score =  525 bits (1352), Expect = e-146
 Identities = 262/352 (74%), Positives = 300/352 (85%)
 Frame = +3

Query: 135  MAEIEEGSPEVRNKQVIFKDYVYGYPKESDMVVTSDATIRLELPKNDGGLILSKNLYLSC 314
            M  +EE   EV NKQ+I K+YV G+PK SDMVV +  TI L+LP+     IL KNLYLSC
Sbjct: 1    MEVVEE---EVGNKQIILKEYVSGFPKGSDMVVKT-GTIHLQLPEGSKNAILVKNLYLSC 56

Query: 315  DPYMRGRMTKTFEGSYTTSFIPGSPISGYGVAKVLDSTHPDFKKGDLISGSTNWEEYSLI 494
            DPYM  RM K  E SY  SF PGSPI+G+GVA+VLDS HP+FKKGDLI G T WEEY++I
Sbjct: 57   DPYMCRRMRK-LEDSYVESFTPGSPITGFGVARVLDSGHPNFKKGDLIWGRTGWEEYTII 115

Query: 495  TNPQSLIKIQHTDVPLSYHTGILGMAGMTAYVGFYEICSPKKGDTVFVSAASGAVGQLVG 674
            T P ++ KIQ+TDVPLSY+TGILGM GMTAYVGFYEICSPK+GD VF+SAASGAVGQLVG
Sbjct: 116  TEPANVFKIQNTDVPLSYYTGILGMHGMTAYVGFYEICSPKQGDYVFISAASGAVGQLVG 175

Query: 675  QFAKLFGCYVVGSAGSKEKVDLLKNKFGFDEAFNYKEEQDLDAALKRYFPDGIDIYFENV 854
            QFAKL GCYVVGSAG+KEKVDLLKNKFGFDEAFNYKEE+DL+AALKRYFP+GI+IYFENV
Sbjct: 176  QFAKLLGCYVVGSAGTKEKVDLLKNKFGFDEAFNYKEEKDLEAALKRYFPNGINIYFENV 235

Query: 855  GGKMLDEVLHNMRLHGRIAVCGMISQYNIDRHEGIYNLAFLILKRIRMQGFIVLDYYHLY 1034
            GGKMLD VL NMRLH RIAVCGMISQYN++R EG++NL  LI KR+RM+GFIV DYYHLY
Sbjct: 236  GGKMLDAVLANMRLHSRIAVCGMISQYNLERPEGVHNLFCLITKRVRMEGFIVFDYYHLY 295

Query: 1035 PKFLDMILPLIQERKITYVEDIVEGLENAPAALVGLFTGKNVGKQVVAIAHE 1190
            PKFL+MILP I+  KITYVED+ EGLE+APAAL+GLF+G+NVGKQVV +A E
Sbjct: 296  PKFLEMILPCIKGGKITYVEDVAEGLESAPAALIGLFSGRNVGKQVVVVARE 347


>ref|XP_002331649.1| predicted protein [Populus trichocarpa] gi|222874045|gb|EEF11176.1|
            predicted protein [Populus trichocarpa]
          Length = 348

 Score =  509 bits (1311), Expect = e-142
 Identities = 248/342 (72%), Positives = 292/342 (85%)
 Frame = +3

Query: 165  VRNKQVIFKDYVYGYPKESDMVVTSDATIRLELPKNDGGLILSKNLYLSCDPYMRGRMTK 344
            V NKQVIFKDYV G  KESDM +T+ +TI+L++P++    +L KNLYLSCDPYMR RM +
Sbjct: 9    VSNKQVIFKDYVPGVLKESDMYITT-STIKLKVPEDCTNGVLVKNLYLSCDPYMRNRM-R 66

Query: 345  TFEGSYTTSFIPGSPISGYGVAKVLDSTHPDFKKGDLISGSTNWEEYSLITNPQSLIKIQ 524
             F+GSY +   PGSPISG GVAKVLDS HPD+KKGD I G T WEEYSLIT  ++L KI 
Sbjct: 67   NFQGSYISPLKPGSPISGRGVAKVLDSRHPDYKKGDFIWGMTGWEEYSLITATETLFKIH 126

Query: 525  HTDVPLSYHTGILGMAGMTAYVGFYEICSPKKGDTVFVSAASGAVGQLVGQFAKLFGCYV 704
              DVPLSY+TGILGM G+TAY GFYEICSPKKG+ VF+SAASGAVGQLVGQFAKL GCYV
Sbjct: 127  DKDVPLSYYTGILGMPGLTAYAGFYEICSPKKGEFVFISAASGAVGQLVGQFAKLLGCYV 186

Query: 705  VGSAGSKEKVDLLKNKFGFDEAFNYKEEQDLDAALKRYFPDGIDIYFENVGGKMLDEVLH 884
            VGSAGSK+KVDLLKNKFGFD+AFNYKEE DLDAALKRYFPDGIDIYFENVGGKMLD V+ 
Sbjct: 187  VGSAGSKDKVDLLKNKFGFDDAFNYKEELDLDAALKRYFPDGIDIYFENVGGKMLDAVVL 246

Query: 885  NMRLHGRIAVCGMISQYNIDRHEGIYNLAFLILKRIRMQGFIVLDYYHLYPKFLDMILPL 1064
            NMR+ GRI+VCGM+SQYN+++ EG++NL  L+ KRI M+GF+V D++HL+PK+LDM+LP 
Sbjct: 247  NMRVRGRISVCGMVSQYNLEQPEGVHNLMHLVPKRIHMEGFLVYDFFHLFPKYLDMVLPY 306

Query: 1065 IQERKITYVEDIVEGLENAPAALVGLFTGKNVGKQVVAIAHE 1190
            I++ KI YVEDI EGLENAPAAL GLF G+N+GKQVVA++HE
Sbjct: 307  IKQGKIVYVEDIAEGLENAPAALTGLFAGRNIGKQVVAVSHE 348


>pdb|4HFJ|A Chain A, X-ray Crystal Structure Of A Double Bond Reductase From
            Nicotiana Tabacum gi|444302247|pdb|4HFJ|B Chain B, X-ray
            Crystal Structure Of A Double Bond Reductase From
            Nicotiana Tabacum gi|444302248|pdb|4HFM|A Chain A, X-ray
            Crystal Structure Of A Nadp(h)-bound Double Bond
            Reductase From Nicotiana Tabacum gi|444302249|pdb|4HFM|B
            Chain B, X-ray Crystal Structure Of A Nadp(h)-bound
            Double Bond Reductase From Nicotiana Tabacum
            gi|444302250|pdb|4HFN|A Chain A, X-ray Crystal Structure
            Of A Ternary Complex Of Double Bond Reductase From
            Nicotiana Tabacum gi|444302251|pdb|4HFN|B Chain B, X-ray
            Crystal Structure Of A Ternary Complex Of Double Bond
            Reductase From Nicotiana Tabacum
          Length = 351

 Score =  509 bits (1310), Expect = e-142
 Identities = 248/343 (72%), Positives = 292/343 (85%)
 Frame = +3

Query: 162  EVRNKQVIFKDYVYGYPKESDMVVTSDATIRLELPKNDGGLILSKNLYLSCDPYMRGRMT 341
            EV NKQVI K+YV GYPKESDM +  + TI+L++P+    +++ KNLYLSCDPYMR RM 
Sbjct: 4    EVSNKQVILKNYVTGYPKESDMEI-KNVTIKLKVPEGSNDVVV-KNLYLSCDPYMRSRMR 61

Query: 342  KTFEGSYTTSFIPGSPISGYGVAKVLDSTHPDFKKGDLISGSTNWEEYSLITNPQSLIKI 521
            K  EGSY  SF PGSPI+GYGVAKVL+S  P F+KGDL+ G T WEEYS+IT  Q+L KI
Sbjct: 62   K-IEGSYVESFAPGSPITGYGVAKVLESGDPKFQKGDLVWGMTGWEEYSIITPTQTLFKI 120

Query: 522  QHTDVPLSYHTGILGMAGMTAYVGFYEICSPKKGDTVFVSAASGAVGQLVGQFAKLFGCY 701
               DVPLSY+TGILGM GMTAY GF+E+CSPKKG+TVFVSAASGAVGQLVGQFAK+ GCY
Sbjct: 121  HDKDVPLSYYTGILGMPGMTAYAGFHEVCSPKKGETVFVSAASGAVGQLVGQFAKMLGCY 180

Query: 702  VVGSAGSKEKVDLLKNKFGFDEAFNYKEEQDLDAALKRYFPDGIDIYFENVGGKMLDEVL 881
            VVGSAGSKEKVDLLK+KFGFDEAFNYKEEQDL AALKRYFPDGIDIYFENVGGKMLD VL
Sbjct: 181  VVGSAGSKEKVDLLKSKFGFDEAFNYKEEQDLSAALKRYFPDGIDIYFENVGGKMLDAVL 240

Query: 882  HNMRLHGRIAVCGMISQYNIDRHEGIYNLAFLILKRIRMQGFIVLDYYHLYPKFLDMILP 1061
             NM+L+GRIAVCGMISQYN+++ EG++NL  LI KRIRM+GF+V DYYHLYPK+L+M++P
Sbjct: 241  VNMKLYGRIAVCGMISQYNLEQTEGVHNLFCLITKRIRMEGFLVFDYYHLYPKYLEMVIP 300

Query: 1062 LIQERKITYVEDIVEGLENAPAALVGLFTGKNVGKQVVAIAHE 1190
             I+  K+ YVED+  GLE+AP ALVGLF+G+N+GKQVV ++ E
Sbjct: 301  QIKAGKVVYVEDVAHGLESAPTALVGLFSGRNIGKQVVMVSRE 343